organism: human gut metagenome
attributes.tag: collection_date
attributes.value: not applicable
attributes.tag: env_broad_scale
attributes.value: not applicable
attributes.tag: env_local_scale
attributes.value: not applicable
attributes.tag: env_medium
attributes.value: not applicable
attributes.tag: geo_loc_name
attributes.value: not applicable
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: lat_lon
attributes.value: not applicable
attributes.tag: library_id
attributes.value: DonorC_S78_L001
attributes.tag: title
attributes.value: 16S rRNA sequencing of fecal sample A3
attributes.tag: library_strategy
attributes.value: AMPLICON
attributes.tag: library_source
attributes.value: GENOMIC
attributes.tag: library_selection
attributes.value: PCR
attributes.tag: library_layout
attributes.value: paired
attributes.tag: platform
attributes.value: ILLUMINA
attributes.tag: instrument_model
attributes.value: Illumina MiSeq
attributes.tag: design_description
attributes.value: V3-V4 16S rRNA amplicon gene sequencing was performed at the Genomics Core Facility of the University of Nebraska Medical Center using Illumina MiSeq utilizing the 600 cycle v3 MiSeq kit. The following primer sequences were used for the 16S library prep protocol: Forward Primer = 5'TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG; Reverse Primer = 5'GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC
attributes.tag: filetype
attributes.value: fastq
attributes.tag: filename
attributes.value: DonorC_S78_L001_R1_001.fastq
attributes.tag: filename2
attributes.value: DonorC_S78_L001_R2_001.fastq
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.human-gut