organism: Pimephales promelas
attributes.tag: strain
attributes.value: GLTED-Duluth-wt
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: breed
attributes.value: not applicable
attributes.tag: cultivar
attributes.value: not applicable
attributes.tag: ecotype
attributes.value: not applicable
attributes.tag: age
attributes.value: 5-6 dpf
attributes.tag: dev_stage
attributes.value: larvae
attributes.tag: collection_date
attributes.value: 2021
attributes.tag: geo_loc_name
attributes.value: USA: Minnesota, Duluth
attributes.tag: sex
attributes.value: not determined
attributes.tag: tissue
attributes.value: whole body
attributes.tag: biomaterial_provider
attributes.value: US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804
attributes.tag: birth_location
attributes.value: US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804
attributes.tag: breeding_method
attributes.value: All larval fathead minnows (Pimephales promelas) used for testing were obtained from an on-site culture facility at the US EPA Great Lakes Toxicology and Ecology Division in Duluth, MN, USA. Fathead minnow embryos were collected from multiple crosses of at least three females in paired breeding. Fertilized embryos that were attached to polyvinyl chloride breeding tiles were transferred to a bath of aerated Lake Superior water and held at 25 °C. All embryos were fertilized within a two-hour window. At five-day post-fertilization, larvae that had hatched and were freely swimming were collected, pooled, and randomly sorted for use. All procedures involving larval fathead minnows were approved by an animal care and use committee in accordance with the Guide for the Care and Use of Laboratory Animals (National Research Council, 2011; US EPA Animal Care and Use Plan # Eco24-09-001).
attributes.tag: collected_by
attributes.value: Great Lakes Toxicology & Ecology Division
attributes.tag: sample_type
attributes.value: Total RNA
attributes.tag: store_cond
attributes.value: -80 deg C
attributes.tag: exposure_duration
attributes.value: 24 h
attributes.tag: measured_conc
attributes.value: 0.04
attributes.tag: measured_conc_units
attributes.value: mg/L
attributes.tag: nominal_conc
attributes.value: 0.1
attributes.tag: nominal_conc_units
attributes.value: mg/L
attributes.tag: extrapolated_conc
attributes.value: -
attributes.tag: extrapolated_conc_units
attributes.value: mg/L
attributes.tag: treatment
attributes.value: methoxyfenozide (DTXSID3032628)
attributes.tag: plate_ID
attributes.value: plate1
attributes.tag: replicate_ID
attributes.value: S68
attributes.tag: BioSampleModel
attributes.value: Model organism or animal