title: Adenolobus_pechuelii_82389_1
organism: Adenolobus pechuelii
description: Protocols: The seeds were obtained from U.S. National Plant Germplasm System Seeds were germinated in Petri dishes in the temperature optimal for each species and transferred to the pots in the cultivation room (photoperiod: 16h/8h, temperature: 22°C, humidity: 70 – 80%). No symbiotic bacteria was added to the soil. RNA isolation was carried out according to the manufacturer's protocol of Spectrum Plant Total RNA Kit (Sigma Aldrich) with minor modifications. Optimization of the protocol included decreasing the amount of ground tissue used to extraction (50 mg instead of proposed 100 mg). The incubation was performed for 5 min at 56°C. Moreover, 750 μL of the binding solution (instead of the proposed 500μL) was added. The DNase digestion was incorporated with the use of the protocol On-Column DNase I Digestion Set (Sigma Aldrich). RNA concentration and quality was measured with the use of Experion Automated Electrophoresis System, RNA StdSens Analysis Kit (Bio-Rad).High-quality DNA was isolated with the use of DNeasy Plant Pro Kit (Qiagen) with no changes to the protocol made. DNA yield has been estimated by nanodrop (ThermoFisher) and quality on 2% agarose gel. RNA libraries were prepared using TruSeq RNA Stranded mRNA.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-07-01T00:20:05Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-07-01T00:20:05Z
attributes.tag: External Id
attributes.value: SAMEA14456150
attributes.tag: INSDC center alias
attributes.value: Institute of Plant Genetics, Polish Academy of Sciences
attributes.tag: INSDC center name
attributes.value: Institute of Plant Genetics, Polish Academy of Sciences
attributes.tag: INSDC first public
attributes.value: 2023-07-01T00:20:05Z
attributes.tag: INSDC last update
attributes.value: 2023-07-01T00:20:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11756:Adenolobus_pechuelii_82389_1
attributes.tag: age
attributes.value: 92
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: sporophyte vegetative stage
attributes.tag: ecotype
attributes.value: 82389
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: shoot system
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-11756:Adenolobus_pechuelii_82389_1
attributes.tag: scientific_name
attributes.value: Adenolobus pechuelii