title: Opto-combinatorial indexing, Paclitaxel treatment experiment
organism: Homo sapiens
description: Protocols: HeLa (RCB0007, RIKEN BRC) cells were maintained in Dulbecco's Modified Eagle Medium (Nacalai Tesque) containing 10% fetal bovine serum (gibco) and 1% penicillin-streptomycin (Sigma-Aldrich). Each cell was stained by CellTrace (Invitrogen™) and transfected with 20 nM barcoded DNA tags by lipofection (4 h incubation).  Cells were cultured with paclitaxel (163-28163, FUJIFILM) at different concentrations for one day. mRNA and DNA tags were collected by colour-coded hydrogel beads from the cell pool in microwells. RNA-derived and DNA tag-derived cDNAs were fractionated with SPRIselect beads (B23318, Beckman Coulter) after PCR amplification.
attributes.tag: isolate
attributes.value: Mixture of mRNA from the HeLa cells obtained by Opto-combinatirial indexing
attributes.tag: age
attributes.value: not applicable
attributes.tag: biomaterial_provider
attributes.value: RIKEN BRC
attributes.tag: collection_date
attributes.value: not applicable
attributes.tag: geo_loc_name
attributes.value: not applicable
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: cervix
attributes.tag: cell_line
attributes.value: HeLa
attributes.tag: disease
attributes.value: cervical carcinoma
attributes.tag: sample_type
attributes.value: RNA and DNA tag
attributes.tag: treatment
attributes.value: Paclitaxel treatment at different concentrations ranging from 0.5 to 500 nM for 24-h
attributes.tag: BioSampleModel
attributes.value: Human