title: Sample 12
organism: Arabidopsis thaliana
description: Protocols: Samples were collected and directly frozen in liquid nitrogen Plants were grown in hydroponics under short-days conditions. Total RNA were isolated using DirectZol RNA kit (ZymoResearch) After the QC procedures, mRNA is enriched using oligo(dT) beads. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
attributes.tag: ENA first public
attributes.value: 2024-01-31
attributes.tag: INSDC center name
attributes.value: Institute for Plant Sciences of Montpellier CNRS
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13661:Sample 12
attributes.tag: age
attributes.value: 5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: rosette growth stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: root
attributes.tag: sample name
attributes.value: E-MTAB-13661:Sample 12
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana
attributes.tag: stimulus
attributes.value: elevated CO2 (900 ppm)
attributes.tag: ecotype
attributes.value: Col-0