title: Human hepatocyes CYN3
organism: Homo sapiens
description: Protocols: HepaRG and HepaG2 cells treated with MC-LR (50 nM) or CYN (50 nM) for 72 h in triplicatetriplicate and samples were collected. Total RNA was isolated using TRIZOl reagents (Sigma). RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer's instructions (NEB, Ipswich, MA, USA). Briefly, mRNAs were first enriched with Oligo(dT) beads. Enriched mRNAs were fragmented for 15 minutes at 94 °C. First strand and second strand cDNAs were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by limited-cycle PCR. The sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-05-12T00:17:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-05-12T00:17:25Z
attributes.tag: External Id
attributes.value: SAMEA113320353
attributes.tag: INSDC center name
attributes.value: North Carolina Central University
attributes.tag: INSDC first public
attributes.value: 2023-05-12T00:17:25Z
attributes.tag: INSDC last update
attributes.value: 2023-05-12T00:17:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12881:Human hepatocyes CYN3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: hepatocyte
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-12881:Human hepatocyes CYN3
attributes.tag: scientific_name
attributes.value: Homo sapiens