title: H3K9me3_ChIP_piwi6KO#10_2
organism: Aedes albopictus
description: ChIP-seq libraries were prepared with the Kapa Hyper Prep Kit for Illumina sequencing (Kapa Biosystems) according to the manufacturer's protocol with the following modifications. 3 ng DNA was used as input, with NEXTflex adapters (Bioo Scientific) and ten cycles of PCR amplification. Post-amplification cleanup and size selection was performed with dual AMPure XP beads selection (0.6× and 0.8×). Size-selected samples were analyzed for purity with a High Sensitivity DNA Chip on a Bioanalyzer 2100 system (Agilent), with an average size between 300 and 500 bp.
attributes.tag: strain
attributes.value: U4.4 cells
attributes.tag: isolate
attributes.value: Piwi6 KO#10 U4.4 cells
attributes.tag: breed
attributes.value: not applicable
attributes.tag: cultivar
attributes.value: not applicable
attributes.tag: ecotype
attributes.value: not applicable
attributes.tag: age
attributes.value: not applicable
attributes.tag: dev_stage
attributes.value: not applicable
attributes.tag: collection_date
attributes.value: 2021-12-10
attributes.tag: geo_loc_name
attributes.value: Netherlands
attributes.tag: sex
attributes.value: not applicable
attributes.tag: tissue
attributes.value: not applicable
attributes.tag: cell_line
attributes.value: U4.4 cell line
attributes.tag: cell_subtype
attributes.value: Piwi6 Knockout clone#10
attributes.tag: collected_by
attributes.value: ET
attributes.tag: phenotype
attributes.value: PIWI6 Knockout clone#10
attributes.tag: treatment
attributes.value: H3K9me3-ChIP
attributes.tag: replicate
attributes.value: biological replicate=2
attributes.tag: library type
attributes.value: ChIP-seq
attributes.tag: BioSampleModel
attributes.value: Model organism or animal