title: E2
organism: Clostridium ljungdahlii
description: Protocols: cell pellets from 50 ml culture were collected by centrifugation at –4 °C and 9,000 rpm (Hitachi CF16RXII, Qingdao, China) for 5 min. Afterwards, pellets were frozen in liquid nitrogen and stored at–80°C. Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer's protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) ≥ 7 were subjected to the subsequent analysis. Double stranded cDNA was generated using the SuperScript Double-Stranded cDNA synthesis kit (Invitrogen) according to the manufacturer's instructions.It was performed by Allwegenetech Corp. (Beijing, China) The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. This part of experiment is carried out at Allwegenetech Corp. (Beijing, China).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-27
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-27
attributes.tag: External Id
attributes.value: SAMEA113577859
attributes.tag: INSDC center alias
attributes.value: Shandong Provincial Key Laboratory of Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
attributes.tag: INSDC center name
attributes.value: Shandong Provincial Key Laboratory of Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
attributes.tag: INSDC first public
attributes.value: 2023-06-27T00:17:08Z
attributes.tag: INSDC last update
attributes.value: 2023-06-27T00:17:08Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13021:E2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: genotype
attributes.value: ΔadhE1
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-13021:E2
attributes.tag: scientific_name
attributes.value: Clostridium ljungdahlii