title: 835
organism: Mus musculus
description: Protocols: Mice were euthanized by increasing concentrations of carbon dioxide. Single-cell suspensions from spleens were prepared using the GentleMacs system (Miltenyi Biotech, Bergisch Gladbach, Germany). Splenocytes were enriched in TCL1 cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) for further analyses, resulting in a purity above 97% of CD5+ CD19+ cells. Single-cell suspensions from spleens were prepared using the GentleMacs system (Miltenyi Biotech, Bergisch Gladbach, Germany). Splenocytes were enriched in TCL1 cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) for further analyses, resulting in a purity above 97% of CD5+ CD19+ cells, or cryopreserved and thawed when necessary. RNA isolation was performed according to manufacturer's recommendations using QIAshredder and RNeasy Mini Kit (both Qiagen, Hilden, Germany). For RNA sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA Kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-01-31T00:29:47Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-01-31T00:29:47Z
attributes.tag: External Id
attributes.value: SAMEA113577882
attributes.tag: INSDC center name
attributes.value: German Cancer Research Center
attributes.tag: INSDC first public
attributes.value: 2024-01-31T00:29:47Z
attributes.tag: INSDC last update
attributes.value: 2024-01-31T00:29:47Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13030:835
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not provided
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genetic modification
attributes.value: none
attributes.tag: genotype
attributes.value: Eµ-TCL1–transgenic
attributes.tag: geographic location (country and/or sea)
attributes.value: not provided
attributes.tag: immunophenotype
attributes.value: CD5+ CD19+ TCL1
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: spleen
attributes.tag: sample name
attributes.value: E-MTAB-13030:835
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J