title: OP_N2_5
organism: Caenorhabditis elegans
description: Protocols: Twenty young adult animals were picked to the fresh plates and allowed to lay eggs for 2 hours, after which the adult animals were removed and eggs were allowed to develop into L4 stage. Ten plates of L4 animals were washed off and placed on either OP50 or V. cholerae containing plates for 8 hours. Subsequently, the animals were collected and rinsed three times with M9 buffer. After washing, the worm pellet was frozen in liquid nitrogen. Animals were homogenized using Bullet Blender (Next Advance) in the presence of Qiazol Lysis Reagent and 0.5mm Zirconia beads at 4°C. RNA was prepared using RNeasy Plus Universal Mini Kit (Qiagen). Library was constructed and sequenced by Novogene
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-10-12T00:21:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-10-12T00:21:14Z
attributes.tag: External Id
attributes.value: SAMEA113546316
attributes.tag: INSDC center name
attributes.value: Umea university, department of molecular biology
attributes.tag: INSDC first public
attributes.value: 2023-10-12T00:21:14Z
attributes.tag: INSDC last update
attributes.value: 2023-10-12T00:21:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13001:OP_N2_5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: L4 larva
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: mixed pool of 20 individuals
attributes.tag: infect
attributes.value: Escherichia coli OP50
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-13001:OP_N2_5
attributes.tag: scientific_name
attributes.value: Caenorhabditis elegans
attributes.tag: sex
attributes.value: mixed
attributes.tag: strain
attributes.value: N2