title: XYT-WT-04
organism: Mus musculus
description: Protocols: Livers were dissected and flash frozen in liquid nitrogen then subsequently stored at -80 degrees C.  Frozen livers were pulverized with a cryoprep, and a portion of frozen liver powder was lysed in a hypotonic PBS-based buffer with Igepal. During cell lysis, samples were dounced to liberate nuclei and subsequently fixed with 85% MeOH and DTSSP.  Fixed nuclei were distributed across wells of a 96-well plate, with individual mice indexed by RT well, and subjected to sci-RNA-seq3.
attributes.tag: ENA first public
attributes.value: 2024-10-15
attributes.tag: INSDC center name
attributes.value: DKFZ
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13700:XYT-WT-04
attributes.tag: age
attributes.value: 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: all liver types
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Y-WT Chr3-WT
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: 1337543
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-13700:XYT-WT-04
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL6/J