title: 21-032_11_mll2CM_H3K27me3_rep2
organism: Mus musculus
description: Protocols: Naive murine embryonic stem cells (mESC) were derived freshly (FVB or mixed 129/B6) and were routinely cultured on gelatin-coated plates in t2i/L media: NDiff (N2B27) (Takara #y40002) supplemented with titrated 2i (0.2__M PD0325901 and 3__M CHIR 99021), 1,000_U/ml leukaemia inhibitory factor (LIF), 1% FBS, 1% penicillin streptomycin and maintained in humidified atmosphere at 37oC and 5% CO2. Cells were passaged every 2_days by dissociation with TrypLE and medium was changed daily. This protocol has been optimised from Skene and Henikoff, Elife, 2017 to detect interaction between DNA and histone modifications. Briefly, fresh cells are harvested, permeabilised, bound to concanavallin magnetic beads and incubated with the antibody for the modification of interest. Samples were then incubated with pA-MNase to cut the DNA side of the bound antibody and the DNA fragments were eluted and subjected to size selection. DNA library was generated with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-05-14T00:15:01Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-05-14T00:15:01Z
attributes.tag: External Id
attributes.value: SAMEA110644491
attributes.tag: INSDC center name
attributes.value: EMBL
attributes.tag: INSDC first public
attributes.value: 2023-05-14T00:15:01Z
attributes.tag: INSDC last update
attributes.value: 2023-05-14T00:15:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12100:21-032_11_mll2CM_H3K27me3_rep2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: mESC
attributes.tag: cell_type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genetic modification
attributes.value: targeted mutation
attributes.tag: genotype
attributes.value: homozygous mll2[Y2602A]
attributes.tag: sample name
attributes.value: E-MTAB-12100:21-032_11_mll2CM_H3K27me3_rep2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: FVB