title: R8-CH4_low_ox_ch4_R7_8
organism: biofilm metagenome
description: microbial biofilm sample from membrane bioreactor treating wastwater, low oxygen reactor, methane membrane, not inoculated
attributes.tag: isolation_source
attributes.value: wastewater bioreactor
attributes.tag: collection_date
attributes.value: 2021-02-21
attributes.tag: geo_loc_name
attributes.value: USA: Ithaca, NY
attributes.tag: lat_lon
attributes.value: not applicable
attributes.tag: rel_to_oxygen
attributes.value: microaerophilic
attributes.tag: samp_collect_device
attributes.value: 2 cm segments of membrane-containing biofilm were sliced and resuspended in 5 mL of Rnase, Dnase-free water. Sample spun down and pellets frozen in -80C before processing.
attributes.tag: samp_mat_process
attributes.value: Biofilm samples collected in 2-mL tubes with 1 mL of DNase, RNase free water, vortexed, pelleted and stored at -80°C before use. DNA was extracted using Trizol, DNA was PCR amplified with primer set 515F-806R, using Q5® Hot Start High-Fidelity 2X Master Mix. Sequencing was performed using the Illumina MiSeq platform with 2 × 250 paired end read lengths
attributes.tag: samp_size
attributes.value: 2 cm segment of biofilm
attributes.tag: source_material_id
attributes.value: R8-CH4_low_ox_ch4_R7_8
attributes.tag: reactor name
attributes.value: R8
attributes.tag: gas type
attributes.value: CH4
attributes.tag: gas level
attributes.value: low
attributes.tag: state
attributes.value: ox
attributes.tag: Date
attributes.value: 8
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental