title: HiG1062
organism: Mus musculus
description: Protocols: Fetal liver cells (FLCs) were isolated from pregnant wildtype CD45.2+ C57Bl/6 females between days 13.5 – 16.5 of gestation. The FLCs were transduced with either empty GFP vector or hnRNP K GFP plasmid. The transduced cells were then sorted for GFP positivity using flow cytometry. RNA was extracted and purified from GFP+ sorted FLCs using Zymo Quick-RNA columns (Zymo Research, Irvine, CA). Barcoded, Illumina-stranded total RNA libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA). Briefly, 250 ng of DNase I-treated total RNA was depleted of cytoplasmic and mitochondrial ribosomal RNA (rRNA) using Ribo-Zero Gold (Illumina, San Diego, CA). After purification, RNA was fragmented using divalent cations and double stranded cDNA was synthesized using random primers. The ends of the resulting double stranded cDNA fragments were repaired, 5′-phosphorylated, 3'-A tailed and Illumina-specific indexed adapters are were ligated. The products were purified and enriched by 12 cycles of PCR to create the final cDNA library. The libraries were quantified by qPCR and assessed for size distribution using the 4200 TapeStation High Sensitivity D1000 ScreenTape (Agilent Technologies, Santa Clara, CA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-10
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-10
attributes.tag: External Id
attributes.value: SAMEA110190670
attributes.tag: INSDC center alias
attributes.value: MD Anderson
attributes.tag: INSDC center name
attributes.value: MD Anderson
attributes.tag: INSDC first public
attributes.value: 2023-06-10T00:18:14Z
attributes.tag: INSDC last update
attributes.value: 2023-06-10T00:18:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11886:HiG1062
attributes.tag: age
attributes.value: 13.5 to 16.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: fetal liver hematopoietic progenitor cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: genotype
attributes.value: hnRNP K GFP overexpression
attributes.tag: immunophenotype
attributes.value: GFP+
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-11886:HiG1062
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6J