title: Sample 1
organism: Homo sapiens
description: Protocols: Adherent cells were harvested in Trizol reagent NA Trizol protocol according to manufactorer's guidelines. At least 800 ng RNA was sent for Quality control and RNA Sequencing Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers,followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library (for further information check Novogene RNA seq protocols).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-10-12T00:21:06Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-10-12T00:21:06Z
attributes.tag: External Id
attributes.value: SAMEA112769151
attributes.tag: INSDC center name
attributes.value: SAARLAND UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2023-10-12T00:21:06Z
attributes.tag: INSDC last update
attributes.value: 2023-10-12T00:21:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12747:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: SH-SY5Y
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: juvenile stage
attributes.tag: disease
attributes.value: neuroblastoma
attributes.tag: genotype
attributes.value: CRISPR-Cas 9 gene deletion STIM1 and STIM2
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: bone
attributes.tag: sample name
attributes.value: E-MTAB-12747:Sample 1
attributes.tag: scientific_name
attributes.value: Homo sapiens