title: Sample 10
organism: Homo sapiens
description: Protocols: Adherent cells were harvested in Trizol reagent NA Trizol protocol according to manufactorer's guidelines. At least 800 ng RNA was sent for Quality control and RNA Sequencing Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers,followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library (for further information check Novogene RNA seq protocols).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-10-12T00:21:06Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-10-12T00:21:06Z
attributes.tag: External Id
attributes.value: SAMEA112769150
attributes.tag: INSDC center name
attributes.value: SAARLAND UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2023-10-12T00:21:06Z
attributes.tag: INSDC last update
attributes.value: 2023-10-12T00:21:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12747:Sample 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: HEK293
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: CRISPR-Cas 9 gene deletion STIM1 and STIM
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: kidney
attributes.tag: sample name
attributes.value: E-MTAB-12747:Sample 10
attributes.tag: scientific_name
attributes.value: Homo sapiens