title: DNA extraction kit reagent
organism: blank sample
description: Negative control sample, reagent from DNA extraction kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-06-22T01:06:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-06-22T01:06:25Z
attributes.tag: External Id
attributes.value: SAMEA110176229
attributes.tag: INSDC center name
attributes.value: Microbial ecology
attributes.tag: INSDC first public
attributes.value: 2024-06-22T01:06:25Z
attributes.tag: INSDC last update
attributes.value: 2024-06-22T01:06:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 2NDNEGCTRLBLK1
attributes.tag: collection date
attributes.value: 2010-12-13
attributes.tag: environment (biome)
attributes.value: anthropogenic environment (ENVO:01000313)
attributes.tag: environment (feature)
attributes.value: laboratory environment (ENVO:01001405)
attributes.tag: environment (material)
attributes.value: laboratory supplies
attributes.tag: geographic location (country and/or sea)
attributes.value: France
attributes.tag: geographic location (depth)
attributes.value: -0.75
attributes.tag: geographic location (elevation)
attributes.value: 318
attributes.tag: geographic location (latitude)
attributes.value: 45.792386
attributes.tag: geographic location (longitude)
attributes.value: 4.706930
attributes.tag: geographic location (region and locality)
attributes.value: Auvergne-Rh�ne-Alpes administrative region, Rh�ne department, Gr�zieu-la-Varenne town
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: pcr primers
attributes.value: ILMN-PTCF2 (GTGCCGYTRTGYGGCAAGA) and ILMN-PTCF2m (GTGCCCYTRTGYGGCAAGT); ILMN-PTCR2 (ATCAKYGCGGCGCGGTCRTA) and ILMNPTCR2m (ATGAGBGCTGCCCTGTCRTA)
attributes.tag: project name
attributes.value: Ecological assessment of combined-sewer overflow practices (PRJEB53724) - Domic 2116
attributes.tag: sample name
attributes.value: 2NDNEGCTRLBLK1
attributes.tag: sampling campaign
attributes.value: not provided
attributes.tag: sampling transect ID
attributes.value: not provided
attributes.tag: scientific_name
attributes.value: blank sample
attributes.tag: sequencing method
attributes.value: Sequencing by synthesis (Illumina MiSeq Illumina V3)
attributes.tag: target gene
attributes.value: Thiopurine S-methyltransferase (tpm)
attributes.tag: target subfragment
attributes.value: 200-235 bp, including the VPLCGK and YDRAAM conserved domains
attributes.tag: water environmental package
attributes.value: water