title: D_GEX
organism: Mus musculus
description: Protocols: To sort astrocytes, mouse neocortices were rapidly harvested then dissociated with Adult Brain Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator using program 37C_ABDK_01. Dissociated samples were then treated with debris and red blood cell removal steps to obtain cell suspensions. For FACS, cells were resuspended in a final volume of 100 uL 0.1% PB buffer. Then cells were incubated with fluorescent conjugated antibodies at 4° C for 30 minutes. The following antibodies were used: ACSA2 APC (1:200, Miltenyi Biotec, 130-116-245) and O4 PE (1:100, Miltenyi Biotec, 30-117-357). ACSA2 APC (+) and O4-PE (-) live single cells were selected as the astrocyte population.   Library preparation was conducted with the Chromium Single Cell 3′ v3.1 Reagent Kit (10x Genomics), following the manufacturer's instructions. Library preparation was conducted with the Chromium Single Cell 3′ v3.1 Reagent Kit (10x Genomics), following the manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2024-10-15
attributes.tag: INSDC center name
attributes.value: Centre for Discovery Brain Sciences, University of Edinburgh
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14233:D_GEX
attributes.tag: age
attributes.value: 10 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: astrocyte
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: WT_APP-WT_FIRE
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: 332
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: library type
attributes.value: Gene expression
attributes.tag: multiplexing cmos
attributes.value: CMO307
attributes.tag: organism part
attributes.value: cerebral cortex
attributes.tag: sample name
attributes.value: E-MTAB-14233:D_GEX
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: CBA