title: Dox Unt Pre-HPCs_2b
organism: Mus musculus
description: Protocols: The doxycycline-inducible ESC line was generated using the inducible cassette exchange method, the constructs and plasmids described in Bergiers et al., 2018; Iacovino et al., 2011; Vargel et al., 2016. Through gene synthesis (GenScript) and cloning, the p2lox-3TFs plasmids was generated and consequently transfected into A2.lox ESCs (kindly provided by Dr. Michael Kyba - University of Minnesota). The deletion of the Tal1 gene to generate the i3TFs Tal1Δ/Δ mESC lines was achieved using the Crispr/CAS9 technology as described in Serina Secanechia et al., 2022. ESCs were maintained, expanded and differentiated as described in Serina Secanechia et al., 2022. Cells were FACS sorted from the hematopoietic 'hemangioblast culture' as described in Serina Secanechia et al., 2022. RNA-seq library preparation and sequencing were performed as described in Picelli et al., 2014 and Serina Secanechia et al., 2022
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-05-15T00:14:50Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-05-15T00:14:50Z
attributes.tag: External Id
attributes.value: SAMEA113400353
attributes.tag: INSDC center name
attributes.value: EMBL
attributes.tag: INSDC first public
attributes.value: 2023-05-15T00:14:50Z
attributes.tag: INSDC last update
attributes.value: 2023-05-15T00:14:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12973:Dox Unt Pre-HPCs_2b
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: i3TFs Tal1Δ/Δ mESCs C3.17
attributes.tag: cell_type
attributes.value: pre-hematopoietic progenitor cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genetic modification
attributes.value: gene knock in combined with biallelic deletion of endogenous Tal1 gene
attributes.tag: genotype
attributes.value: knock in of hematopoietic genes Tal1, Lyl1 and Lmo2 under a dox-inducible promoter in ∆Tal1/∆Tal1 background
attributes.tag: immunophenotype
attributes.value: VE-CADHERIN+CD41+
attributes.tag: progenitor cell type
attributes.value: embryonic stem cell
attributes.tag: sample name
attributes.value: E-MTAB-12973:Dox Unt Pre-HPCs_2b
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: stimulus
attributes.value: doxycycline (1µg/mL)
attributes.tag: stimulus schedule
attributes.value: EB: doxycycline; Hemangioblast: untreated