title: Cdk6_K43M_2
organism: Mus musculus
description: Protocols: Mouse HSC/MPP1 cells were isolated from bone marrow by means of flow cytometry sorting using the following immunophenotypic marker combination: lineage-negative, Sca1-positive, cKit-positive, CD150-positive, CD48-negative. This was part of the library construction protocol. Libraries were constructed following the SmartSeq2 protocol as described in Picelli, S., et al. Nature Protocols 9, 171-181, 2014
attributes.tag: ENA first public
attributes.value: 2024-05-22
attributes.tag: External Id
attributes.value: SAMEA113987302
attributes.tag: INSDC center name
attributes.value: University of Veterinary Medicine Vienna Institute of Pharmacology and Toxicology
attributes.tag: INSDC last update
attributes.value: 2023-07-05T11:48:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13145:Cdk6_K43M_2
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: Kit and Sca1-positive hematopoietic stem cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Cdk6_K43M kinase inactive mutant
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth condition
attributes.value: in vivo transplantation
attributes.tag: immunophenotype
attributes.value: CD45.2 positive
attributes.tag: individual
attributes.value: HSC-MPP1_pool_5
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-13145:Cdk6_K43M_2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: mixed
attributes.tag: strain
attributes.value: C57BL/6