title: G25deltaE1
organism: Bacillus cereus G9241
description: Protocols: Bacterial cultures were grown to exponential phase at 25 or 37˚ degree celsius before samples were taken and RNAprotect (Qiagen) was added to each samples. QIAzol (Qiagen) was added to each pelleted samples before transferring to Lysing Matrix B tubes (MP Biomedicals). Cells were lysed using the FastPrep®-24 Classic instrument (MP Biomedicals) with a COOLPREP™ adapter (MP Biomedicals). Bead beating was conducted at 6 ms-1 for 40 s for 2 cycles, with a 300 s pause between cycles. Lysates were centrifuged for 1 minute at 10,000 g and supernatant extracted. an RNeasy Micro Kit (Qiagen) was then used to extract RNA following manufacturers protocol. TruSeq library prep was conducted using the Low Sample (LS) protocol and manufacturer's instructions were followed which are detailed in the LS protocol of the TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-05-13T00:14:53Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-05-13T00:14:53Z
attributes.tag: External Id
attributes.value: SAMEA113399670
attributes.tag: INSDC center name
attributes.value: The University of Warwick
attributes.tag: INSDC first public
attributes.value: 2023-05-13T00:14:53Z
attributes.tag: INSDC last update
attributes.value: 2023-05-13T00:14:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12883:G25deltaE1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: ∆pBCXO1
attributes.tag: growth condition
attributes.value: liquid LB medium
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-12883:G25deltaE1
attributes.tag: scientific_name
attributes.value: Bacillus cereus G9241