title: Opto-combinatorial indexing, Mixed species experiment (electroporation)
organism: mixed sample
description: Protocols: HeLa (RCB0007, RIKEN BRC) and NIH/3T3 cells (RCB2767, RIKEN BRC) cells were maintained in Dulbecco's Modified Eagle Medium (Nacalai Tesque) containing 10% fetal bovine serum (gibco) and 1% penicillin-streptomycin (Sigma-Aldrich). Each cell was stained by CellTrace (Invitrogen™) and transfected with 100nM barcoded DNA tags by electroporation (250 V, capacitance: 2000 μF, resistance: ∞ Ohm, duration: 20 ms for HeLa cell, and  400 V, capacitance: 950 μF, resistance: ∞ Ohm, duration: 20 ms for NIT/3T3 cell). After incubation in a culture medium for 1 h at 37°C in 5% CO2, mRNA and DNA tags were collected by colour-coded hydrogel beads from the cell pool in microwells. RNA-derived and DNA tag-derived cDNAs were fractionated with SPRIselect beads (B23318, Beckman Coulter) after PCR amplification.
attributes.tag: isolate
attributes.value: Mixture of mRNA from the HeLa and HIH/3T3 cells obtained by Opto-combinatirial indexing
attributes.tag: age
attributes.value: not applicable
attributes.tag: collection_date
attributes.value: not applicable
attributes.tag: geo_loc_name
attributes.value: not applicable
attributes.tag: sex
attributes.value: not applicable
attributes.tag: tissue
attributes.value: not applicable
attributes.tag: cell_line
attributes.value: HeLa and HIH/3T3 cells
attributes.tag: sample_type
attributes.value: RNA and DNA tag
attributes.tag: BioSampleModel
attributes.value: Model organism or animal