title: i5TFs unt late eVSM_1b
organism: Mus musculus
description: Protocols: Doxycycline-inducible ESC lines were generated using the inducible cassette exchange method, the constructs and plasmids described in Bergiers et al., 2018; Iacovino et al., 2011; Vargel et al., 2016. Through gene synthesis (GenScript) and cloning, p2lox-5TFs and p2lox-3TFs plasmids were generated. They were consequently transfected into A2.lox ESCs (kindly provided by Dr. Michael Kyba - University of Minnesota). ESCs were maintained, expanded and differentiated as described in Serina Secanechia et al., 2022 Cell sorting, culture and transcription factor (TF) overexpression were performed as described in Serina Secanechia et al., 2022 RNA-seq library preparation and sequencing were performed as described in Picelli et al., 2014 and Serina Secanechia et al., 2022
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-05-15T00:14:48Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-05-15T00:14:48Z
attributes.tag: External Id
attributes.value: SAMEA112994115
attributes.tag: INSDC center name
attributes.value: EMBL
attributes.tag: INSDC first public
attributes.value: 2023-05-15T00:14:48Z
attributes.tag: INSDC last update
attributes.value: 2023-05-15T00:14:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12957:i5TFs unt late eVSM_1b
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: i5TFs mESC
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genetic modification
attributes.value: gene knock in
attributes.tag: genotype
attributes.value: knock in of hematopoietic genes Runx1, Cbfb, Gata2, Fli1, Erg under a dox-inducible promoter
attributes.tag: immunophenotype
attributes.value: VE-CADHERIN-CD41-
attributes.tag: sample name
attributes.value: E-MTAB-12957:i5TFs unt late eVSM_1b
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male