title: WT_T0_B
organism: Homo sapiens
description: Protocols: 80 million HCC827 cells (WT or RBM10-KO) were collected at each timepoint, snap-frozen in liquid nitrogen, and kept in -80°C prior to genomic DNA extraction. Genomic DNA extraction was performed with Blood & Cell Culture DNA Maxi Kit (Qiagen) according to the manufacturer's instructions. gRNA inserts were amplified using two round of PCR reactions. First, a 600bp region of the lentiviral library vectors containing the sgRNA region was amplified using ordinary PCR with Q5 high-fidelity DNA polymerase for 25 cycles. A total of 120μg gDNA was used as template for each sample (60reactions: 2μg gDNA/50μl reaction). Second, the amplified PCR product was subjected to another PCR reaction with Illumina next-generation sequencing adaptors for 10 cycles and 200bp product was amplified.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-03-05
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-03-05
attributes.tag: External Id
attributes.value: SAMEA112721082
attributes.tag: INSDC center alias
attributes.value: Faculty of Biology Technion - Israel Institute of Technology
attributes.tag: INSDC center name
attributes.value: Faculty of Biology Technion - Israel Institute of Technology
attributes.tag: INSDC first public
attributes.value: 2023-03-05T10:00:06Z
attributes.tag: INSDC last update
attributes.value: 2023-03-05T10:00:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12724:WT_T0_B
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: HCC827
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: lung adenocarcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-12724:WT_T0_B
attributes.tag: scientific_name
attributes.value: Homo sapiens