title: BE-mE-3
organism: Saccharomyces cerevisiae
description: Protocols: Cultured yeast cells were used to isolate mitochondria using a Yeast Mitochondria Isolation Kit (Abcam). mtDNA was extracted from isolated mitochondria using the FastGene Plasmid Mini Kit (Nippon Genetics, Tokyo, Japan) and purified using AMpure XP beads (Beckman Coulter, CA, USA). Extracted gDNA and mtDNA were subjected to library construction using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA).
attributes.tag: strain
attributes.value: S288c
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Saccharomyces cerevisiae
attributes.tag: organism
attributes.value: Saccharomyces cerevisiae
attributes.tag: growth condition
attributes.value: SDC+A (72 hours)
attributes.tag: collection date
attributes.value: not collected
attributes.tag: scientific_name
attributes.value: Saccharomyces cerevisiae
attributes.tag: common name
attributes.value: brewer's yeast
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: fraction
attributes.value: mitochondrial fraction
attributes.tag: genotype
attributes.value: CEN.PK2-1C (Euroscarf: 30005A) pMnC-CDA p426-gRNA-empty
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-01-18