title: Sample 1_GEX
organism: Homo sapiens
description: Protocols: K562 cells and THP-1 cells were purchased from JCRB CellBank (JCRB0019 lot: 06212017, JCRB0112.1 lot:11092017). Cells were grown in RPMI1640 medium (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich) and 1x Antibiotic Antimycotic solution (Thermo Fisher Scientific). Cells were encapsulated in microdroplets with DNA-barcoded beads, where DNA barcodes were released from beads and absorbed by cells. cDNA and DNA barcodes were read in separate sequencing libraries. Droplet-based (Chromium NextGEM SIngle Cell 3' Reagent Kits, 10X) Droplet-based (Chromium NextGEM single Cell 3' Reagent Kits, 10X) used for the GEX libraries. For DNA barcode samples, libraries were also prepared in parallel to cDNA samples using the same 10X kit but generated based on a custom cell-hashing method. This cell-hashing method does not tag samples (one barcode)-by-(one sample) basis. Instead, it tags samples with unique ID generated by combinations of barcodes . See Additional File E-MTAB-12819.vsc for a list of each barcode consisting of the combinatorial IDs.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-27
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-27
attributes.tag: External Id
attributes.value: SAMEA112868271
attributes.tag: INSDC center alias
attributes.value: The University of Tokyo, Institute for Quantitative Biosciences
attributes.tag: INSDC center name
attributes.value: The University of Tokyo, Institute for Quantitative Biosciences
attributes.tag: INSDC first public
attributes.value: 2023-06-27T15:00:48Z
attributes.tag: INSDC last update
attributes.value: 2023-06-27T15:00:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12819:Sample 1_GEX
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: mixed K562 and THP-1
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: mixed chronic myelogenous leukemia and acute monocytic leukemia
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: mixed bone marrow and blood
attributes.tag: sample name
attributes.value: E-MTAB-12819:Sample 1_GEX
attributes.tag: scientific_name
attributes.value: Homo sapiens