title: D22_G12_Patient_REP2_RUN3
organism: Homo sapiens
description: Protocols: Collection of 50000 cells per replicate and resuspension of the pellet in TRIzol buffer - Resuspension of nuclei in the transposition reaction mix- Incubation of the transposition reaction- Purification using Qiagen MinElute PCR Purification Kit- Elution of transposed DNA in Elution BufferRefer to DOI: 10.1002/0471142727.mb2129s109 - Amplification of DNA fragments- Purification of amplified library using Qiagen MinElute PCR Purification Kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-15
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-15
attributes.tag: External Id
attributes.value: SAMEA112655728
attributes.tag: INSDC center alias
attributes.value: Istituto di Ricerche Farmacologiche \"Mario Negri\" IRCCS
attributes.tag: INSDC center name
attributes.value: Istituto di Ricerche Farmacologiche \"Mario Negri\" IRCCS
attributes.tag: INSDC first public
attributes.value: 2023-06-15T00:18:06Z
attributes.tag: INSDC last update
attributes.value: 2023-06-15T00:18:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12661:D22_G12_Patient_REP2_RUN3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: induced pluripotent stem cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: Cornelia de Lange syndrome
attributes.tag: genotype
attributes.value: NIPBL mutant correction
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-12661:D22_G12_Patient_REP2_RUN3
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female