title: EMOBON_IUIEilat_Wa_1
organism: marine plankton metagenome
description: EMOBON metagenome water sample from station IUIEilat collected on 2021-08-29 size fraction 0.2-3um
attributes.tag: ENA first public
attributes.value: 2024-11-11
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00080
attributes.tag: amount or size of sample collected
attributes.value: 10
attributes.tag: broad-scale environmental context
attributes.value: marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023]
attributes.tag: chlorophyll
attributes.value: 0.094167
attributes.tag: chlorophyll method
attributes.value: Trilogy fluorometer - Module: CHL-A NA model # 7200-046
attributes.tag: collection date
attributes.value: 2021-08-29
attributes.tag: depth
attributes.value: 2
attributes.tag: dna concentration
attributes.value: 6.32
attributes.tag: environmental medium
attributes.value: seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
attributes.tag: geographic location (country and/or sea)
attributes.value: Israel
attributes.tag: geographic location (latitude)
attributes.value: 29.5
attributes.tag: geographic location (locality)
attributes.value: Gulf of Eilat
attributes.tag: geographic location (longitude)
attributes.value: 34.916667
attributes.tag: geographic location (ocean)
attributes.value: Indian Ocean
attributes.tag: geographic location (region and locality)
attributes.value: Gulf of Eilat
attributes.tag: library reads sequenced
attributes.value: 82699976
attributes.tag: local environmental context
attributes.value: marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
attributes.tag: mock_eu
attributes.value: DDC_AAABOSDA_4_HMGW5DSX3.UDI213
attributes.tag: mock_pr
attributes.value: DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
attributes.tag: obs_id
attributes.value: IUIEilat
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: relevant standard operating procedures
attributes.value: Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
attributes.tag: sample collection device
attributes.value: Hand pump
attributes.tag: sample material processing
attributes.value: WaSOP 1
attributes.tag: sample name
attributes.value: EMOBON00080
attributes.tag: sample storage location
attributes.value: Eppendorf F570 -80 freezer
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: marine plankton metagenome
attributes.tag: sea surface temperature
attributes.value: 27.4
attributes.tag: sea surface temperature method
attributes.value: Thermistor - Campbell Scientific 108
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch1
attributes.tag: sequence_batch_rundate
attributes.value: Batch1_220622
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: size-fraction lower threshold
attributes.value: 0.2
attributes.tag: size-fraction upper threshold
attributes.value: 3
attributes.tag: source material identifiers
attributes.value: EMOBON_IUIEilat1_Wa_210829_0.2um_1
attributes.tag: status
attributes.value: sample
attributes.tag: subsurface salinity
attributes.value: 40.7649
attributes.tag: subsurface salinity method
attributes.value: MS-315e Salinometer
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage information
attributes.value: no_tide
attributes.tag: total depth of water column
attributes.value: 700