title: Total RNA of CHO-K1 cells, 5x1000
organism: Cricetulus griseus
description: Total RNA from CHO-K1 cells (ATCC: CCL-61) was extracted using RNeasy (QIAGEN) following manufactures' protocol with spike-in 5x1k of model viruses
attributes.tag: sample_name
attributes.value: CHO-K1_RNA_spike-in_5x1000
attributes.tag: age
attributes.value: Pasage 6
attributes.tag: cell_line
attributes.value: CHO-K1
attributes.tag: cell_type
attributes.value: Suspension cells
attributes.tag: collection_date
attributes.value: 2022-12-01
attributes.tag: dev_stage
attributes.value: CHO-K1_RNA
attributes.tag: geo_loc_name
attributes.value: Japan:Osaka
attributes.tag: growth_protocol
attributes.value: CHO-K1 cells were grown in 20 mL of EX-CELL_ CD CHO Fusion (14365C-1000ML; Merck, Darmstadt, Germany) containing 600 L of 200mmol/L L-Glutamine Solution (x100) (073-05391, Fujifilm Wako Pure Chemical, Osaka, Japan) in a 125 mL flask. The flask was shaken (120 rpm) at 37 degree C in a humidified 5% CO2 atmosphere.
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: CHO-K1
attributes.tag: tissue
attributes.value: cell