title: N642HNKNK_3
organism: Mus musculus
description: Protocols: Frozen liver samples from aged non-diseased Cre neg (n=3), GFPNK/NK (n=2), STAT5BNK/NK (n=3) and N642HNK/NK (n=5) mice and from diseased N642HNK/NK mice (n=8) were thawed and (GFP+)CD3-NK1.1+ NK cells were directly sorted into a SMARTSeq lysis buffer with a CytoFlex SRT Sorter (Beckman Coulter).  This was part of the library construction protocol. Libraries were constructed using the SMART-SEQ3 method (Michael Hagemann-Jensen, et al.; Nat Biotechnol. 2020 Jun;38(6):708-714).
attributes.tag: strain
attributes.value: C57BL/6N
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: female
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: common name
attributes.value: house mouse
attributes.tag: organism part
attributes.value: liver
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: STAT5BNK/NK
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: age
attributes.value: 486
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected