title: EMOBON_OOB_So_1
organism: marine sediment metagenome
description: EMOBON metagenome sediment sample from station OOB collected on 2021-06-08 community collected micro
attributes.tag: ENA first public
attributes.value: 2024-11-11
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00001
attributes.tag: broad-scale environmental context
attributes.value: marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
attributes.tag: collection date
attributes.value: 2021-06-08
attributes.tag: depth
attributes.value: 27
attributes.tag: dna concentration
attributes.value: 1.7
attributes.tag: elevation
attributes.value: -27
attributes.tag: environmental medium
attributes.value: sediment [ENVO:00002007]
attributes.tag: geographic location (country and/or sea)
attributes.value: France
attributes.tag: geographic location (latitude)
attributes.value: 42.484167
attributes.tag: geographic location (locality)
attributes.value: Bay of Banyuls-sur-Mer
attributes.tag: geographic location (longitude)
attributes.value: 3.135278
attributes.tag: geographic location (ocean)
attributes.value: Mediterranean Sea - Western Basin
attributes.tag: geographic location (region and locality)
attributes.value: Gulf of Lion
attributes.tag: library reads sequenced
attributes.value: 58079642
attributes.tag: local environmental context
attributes.value: shallow_marine_sediment_[ENVO:03000034]
attributes.tag: mock_eu
attributes.value: DDC_AAABOSDA_1_HWLTKDRXY.UDI213
attributes.tag: mock_pr
attributes.value: DDC_AAAAOSDA_1_HWLTKDRXY.UDI212
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
attributes.tag: obs_id
attributes.value: OOB
attributes.tag: pH
attributes.value: 7.962
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: ph method
attributes.value: Consort multimeter C3016
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: relevant standard operating procedures
attributes.value: Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
attributes.tag: salinity
attributes.value: 37.85
attributes.tag: salinity method
attributes.value: Seabird SBE 911plus CTD
attributes.tag: sample collection device
attributes.value: PVC sediment corer
attributes.tag: sample material processing
attributes.value: SoSOP 2
attributes.tag: sample name
attributes.value: EMOBON00001
attributes.tag: sample storage location
attributes.value: room 213, freezer LECOB
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: marine sediment metagenome
attributes.tag: sea surface temperature
attributes.value: 18.75
attributes.tag: sea surface temperature method
attributes.value: Seabird SBE 911plus CTD
attributes.tag: sediment temperature
attributes.value: 18.66
attributes.tag: sediment temperature method
attributes.value: Consort temperature compensator (ST10) PT1000
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch1
attributes.tag: sequence_batch_rundate
attributes.value: Batch1_220223
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: source material identifiers
attributes.value: EMOBON_OOB_So_210608_micro_1
attributes.tag: status
attributes.value: sample
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage information
attributes.value: no_tide
attributes.tag: total depth of water column
attributes.value: 27