title: GDCA
organism: Solanum tuberosum
description: Protocols: Samples were harvested from 42 day-old plants at the beginning of the day. Three biological replicates, each consisting of a pool of leaves from six plants (the third completely expanded leaf), were collected. S. tuberosum var. Spunta wild type plants were used. Sprouted tubers of similar size were planted in pots of 1000 cm3. Plants were grown in a greenhouse with natural radiation (PAR = 500 mmol m-2 s-1) with temperatures ranging between 20°C and 24°C. Experiments were carried out between March and October with a photoperiod of about 10.5 h of light. *For shade avoidance experiments, plants were grown in a greenhouse with natural radiation for 28 days. Then, plants were transferred to simulated shade provided by the same light sources in combination with two green acetate filters (no. 089; LEE Filters, Hampshire, UK) to reduce blue and red light and the R:FR (red to far red) ratio, or kept in control conditions, for additional 14 days. *For drought stress treatment, plants were watered daily at field capacity for 28 days. Then, water was restricted to 35% of field capacity for 14 days. Total RNA was extracted using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich), according to the manufacturer's instructions. Unstranded libraries were prepared using standard Illumina kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-07-12T00:19:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-07-12T00:19:19Z
attributes.tag: External Id
attributes.value: SAMEA112772495
attributes.tag: INSDC center alias
attributes.value: IFEVA (CONICET-UBA) Facultad de Agronomia Universidad de Buenos Aires (UBA)
attributes.tag: INSDC center name
attributes.value: IFEVA (CONICET-UBA) Facultad de Agronomia Universidad de Buenos Aires (UBA)
attributes.tag: INSDC first public
attributes.value: 2023-07-12T00:19:19Z
attributes.tag: INSDC last update
attributes.value: 2023-07-12T00:19:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12771:GDCA
attributes.tag: age
attributes.value: 42
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: potato
attributes.tag: cultivar
attributes.value: Spunta
attributes.tag: developmental stage
attributes.value: sporophyte vegetative stage
attributes.tag: environmental stress
attributes.value: none
attributes.tag: genotype
attributes.value: BBX21-OE CH2
attributes.tag: growth condition
attributes.value: green house study
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-12771:GDCA
attributes.tag: sampling site
attributes.value: third expanded leaf
attributes.tag: scientific_name
attributes.value: Solanum tuberosum