title: Total RNA of CHK-Q cells, spike-in control
organism: Cricetulus griseus
description: Total RNA from CHK-Q cells (RIKEN CELL BANK ID: RCB5676) was extracted using RNeasy (QIAGEN) following manufactures' protocol without spike-in of model viruses
attributes.tag: sample_name
attributes.value: CHK-Q_RNA_spike-in_control
attributes.tag: age
attributes.value: Passage 40
attributes.tag: cell_line
attributes.value: CHK-Q
attributes.tag: cell_type
attributes.value: Adherent cells
attributes.tag: collection_date
attributes.value: 2022-12-15
attributes.tag: dev_stage
attributes.value: CHK-Q_RNA
attributes.tag: geo_loc_name
attributes.value: Japan:Fukuoka
attributes.tag: growth_protocol
attributes.value: CHK-Q cells were cultured in DMEM/F-12 (DF) medium (042_30555; Fujifilm Wako Pure Chemical, Osaka, Japan) supplemented with 10% fetal bovine serum (FBS) (Biowest, Nuaill_, France) in 100-mm cell culture dishes coated with collagen type I (4020_010; AGC Techno Glass, Shizuoka, Japan)._Cells were cultured at 37 degree C in a 5% (v/v) CO2_incubator (Panasonic Healthcare, Tokyo, Japan) and observed under a BZ-9000 digital microscope (Keyence, Tokyo, Japan). Cells were cultured at 37 degree C in a 5% (v/v) CO2_incubator (Panasonic Healthcare, Tokyo, Japan) and observed under a BZ-9000 digital microscope (Keyence, Tokyo, Japan).
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: CHK-Q
attributes.tag: tissue
attributes.value: cell