title: Donor C CAR.CCR7 cocultured
organism: Homo sapiens
description: Protocols: CAR T cells were generated from PBMCs of healthy donors under IL7&IL15 supplementation. Retroviral transduction with CAR and CAR.CCR7 was performed at day 3&4 respectively after 48 hrs of plate-bound (CD3/CD28) activation. Cells were cultured until day 10 and either cultured over night with or without Jeko1 co-culture. Cells were enriched at an AriaFusion cell sorter by using the fluorescent reporter mTurquoise that is incorporated in the vector backbone. Cells were sorted into a 15mL conical tube with 5mL fully supplemented medium, spun down at 400xg for 5 min. and directly processed for mRNA Seq RNA was harvested using the Uneasy Micro Kit from Qiagen strictly following the manufacturers instructions. All steps were performed on ice and on an RNAse free bench Illumina TruSeq stranded RNA was used to generate libraries. All libraries were generated with 1µg total input RNA per sample
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-30T00:24:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-30T00:24:21Z
attributes.tag: External Id
attributes.value: SAMEA112797766
attributes.tag: INSDC center alias
attributes.value: Massachusetts General Hospital Harvard Medical School Broad Institute
attributes.tag: INSDC center name
attributes.value: Massachusetts General Hospital Harvard Medical School Broad Institute
attributes.tag: INSDC first public
attributes.value: 2023-06-30T00:24:21Z
attributes.tag: INSDC last update
attributes.value: 2023-06-30T00:24:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12778:Donor C CAR.CCR7 cocultured
attributes.tag: age
attributes.value: 30
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: T cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: CAR.CCR7
attributes.tag: growth condition
attributes.value: coculture with Jeko1 cells
attributes.tag: individual
attributes.value: C
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: T cell
attributes.tag: sample name
attributes.value: E-MTAB-12778:Donor C CAR.CCR7 cocultured
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male