title: Sample 1
organism: Homo sapiens
description: Protocols: After culture, cells were rinsed with PBS total RNA was isolated according to TRI Reagent protocol. Cell lines were maintained at 37˚C, 95% humidity and 5% CO2 in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Total RNA was isolated according to TRI Reagent protocol. The SMARTer smRNA-Seq Kit for Illumina is used to generate small RNA-seq libraries for sequencing on iSeq100 Illumina platform. A total of 500ng of total RNA was used as input for library construction. Illumina adapter and index sequences are incorporated in a ligation-free manner during library amplification, ensuring that diverse smRNA species are represented with minimal bias.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-16
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-16
attributes.tag: External Id
attributes.value: SAMEA113482194
attributes.tag: INSDC center alias
attributes.value: Vinca Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade
attributes.tag: INSDC center name
attributes.value: Vinca Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade
attributes.tag: INSDC first public
attributes.value: 2023-06-16T00:26:35Z
attributes.tag: INSDC last update
attributes.value: 2023-06-16T00:26:35Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13009:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: HEK293
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genetic modification
attributes.value: MIR185 knockout
attributes.tag: genotype
attributes.value: CRISPR-Cas9 induced MIR185 gene knockout
attributes.tag: organism part
attributes.value: kidney
attributes.tag: sample name
attributes.value: E-MTAB-13009:Sample 1
attributes.tag: scientific_name
attributes.value: Homo sapiens