title: AA8
organism: Homo sapiens
description: Protocols: HeLa cells were collected after 72hr post transfection. HeLa were purchased from the American Type Culture Collection (ATCC). Cells were cultivated in Glutamax Dulbecco's modified Eagle's medium (Life technologies) supplemented with 10% Fetal bovine serum (Life technologies) and antibiotics (penicillin 500 u/ml; streptomycin 0.5 mg/ml, Life Technologies). HeLa cells were forward transfected in 96-well plates with a siRNA library against known factors and regulators using SMARTpools (ON TARGET PLUS, Thermo-Scientific) using an automatized robotic procedure (Sciclone Liquid Handling Workstation, Perkin Elmer). Endogenous mRNAs were purified 72 hours post transfection by using oligo dT-coated 96 well plates (mRNA catcher PLUS, Life Technologies) following the manufacturer's instructions. Ultra low input library preparation. Full-length RNA-seq from single cells using Smart-seq2
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-12-30T01:19:11Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-12-30T01:19:11Z
attributes.tag: External Id
attributes.value: SAMEA11322526
attributes.tag: INSDC center name
attributes.value: Centre for Genomic Regulation (CRG)
attributes.tag: INSDC first public
attributes.value: 2023-12-30T01:19:11Z
attributes.tag: INSDC last update
attributes.value: 2023-12-30T01:19:11Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11202:AA8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: HeLa
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: cervical carcinoma
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: cervix epithelium
attributes.tag: sample name
attributes.value: E-MTAB-11202:AA8
attributes.tag: scientific_name
attributes.value: Homo sapiens