title: EMOBON_NRMCB_Wa_23
organism: marine plankton metagenome
description: EMOBON metagenome water sample from station NRMCB collected on 2021-08-31 size fraction 3-200um
attributes.tag: ENA first public
attributes.value: 2024-11-11
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00062
attributes.tag: ammonium
attributes.value: 0.41
attributes.tag: ammonium method
attributes.value: segmented continuous-flow autoanalyzer
attributes.tag: amount or size of sample collected
attributes.value: 5
attributes.tag: broad-scale environmental context
attributes.value: marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023];mediterranean sea biome[ENVO_01000047]
attributes.tag: chlorophyll
attributes.value: 1.71
attributes.tag: chlorophyll method
attributes.value: spectrofluorometry
attributes.tag: collection date
attributes.value: 2021-08-31
attributes.tag: conductivity
attributes.value: 46.07
attributes.tag: conductivity method
attributes.value: CTD SBE911plusV2
attributes.tag: density
attributes.value: 25.0316
attributes.tag: density method
attributes.value: CTD SBE911plusV2
attributes.tag: depth
attributes.value: 0
attributes.tag: dissolved oxygen
attributes.value: 4.607
attributes.tag: dissolved oxygen method
attributes.value: SBE 43
attributes.tag: dna concentration
attributes.value: 12.7
attributes.tag: environmental medium
attributes.value: seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
attributes.tag: geographic location (country and/or sea)
attributes.value: Italy
attributes.tag: geographic location (latitude)
attributes.value: 40.800139
attributes.tag: geographic location (locality)
attributes.value: Naples Gulf
attributes.tag: geographic location (longitude)
attributes.value: 14.25
attributes.tag: geographic location (ocean)
attributes.value: Mediterranean Sea - Western Basin
attributes.tag: geographic location (region and locality)
attributes.value: Tyrrhenian Sea
attributes.tag: library reads sequenced
attributes.value: 94643683
attributes.tag: local environmental context
attributes.value: marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
attributes.tag: mock_eu
attributes.value: DDC_AAABOSDA_4_HMGW5DSX3.UDI213
attributes.tag: mock_pr
attributes.value: DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
attributes.tag: nitrate
attributes.value: 0.01
attributes.tag: nitrate method
attributes.value: segmented continuous-flow autoanalyzer
attributes.tag: nitrite
attributes.value: 0.05
attributes.tag: nitrite method
attributes.value: segmented continuous-flow autoanalyzer
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
attributes.tag: obs_id
attributes.value: NRMCB
attributes.tag: organism count
attributes.value: phytoplankton: 4.46E+06; diatoms: 2.76E+06; dinoflagellates: 7.25E+04; coccolitrophores: 0.00E+00; other flagellates: 1.63E+06
attributes.tag: organism count method
attributes.value: phytoplankton; diatoms; dinoflagellates; coccolithophores; other flagellates: Utermöhl (Phytoplankton) Sedimentation Chamber
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: ph method
attributes.value: SBE27
attributes.tag: phaeopigments
attributes.value: 0,51
attributes.tag: phaeopigments method
attributes.value: spectrofluorometry
attributes.tag: phosphate
attributes.value: 0.1
attributes.tag: phosphate method
attributes.value: segmented continuous-flow autoanalyzer
attributes.tag: pigments
attributes.value: 1,2505
attributes.tag: pigments method
attributes.value: this is not pigments but fluorescense. WetLabs ECO-FL
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: relevant standard operating procedures
attributes.value: Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
attributes.tag: salinity
attributes.value: 37.9887
attributes.tag: salinity method
attributes.value: CTD SBE911plusV2
attributes.tag: sample collection device
attributes.value: Niskin
attributes.tag: sample material processing
attributes.value: Wa SOP1
attributes.tag: sample name
attributes.value: EMOBON00062
attributes.tag: sample storage location
attributes.value: Casotti minus80
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: marine plankton metagenome
attributes.tag: sea subsurface temperature
attributes.value: 26.8072
attributes.tag: sea subsurface temperature method
attributes.value: CTD SBE911plusV2
attributes.tag: sea surface temperature
attributes.value: 26.8119
attributes.tag: sea surface temperature method
attributes.value: CTD SBE911plusV2
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch1
attributes.tag: sequence_batch_rundate
attributes.value: Batch1_220622
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: silicate
attributes.value: 0.31
attributes.tag: silicate method
attributes.value: segmented continuous-flow autoanalyzer
attributes.tag: size-fraction lower threshold
attributes.value: 3
attributes.tag: size-fraction upper threshold
attributes.value: 200
attributes.tag: source material identifiers
attributes.value: EMOBON_NRMCB_Wa_210831_3um_1
attributes.tag: status
attributes.value: sample
attributes.tag: subsurface salinity
attributes.value: 37.9916
attributes.tag: subsurface salinity method
attributes.value: CTD SBE911plusV2
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage information
attributes.value: no_tide
attributes.tag: total depth of water column
attributes.value: 75
attributes.tag: turbidity
attributes.value: 0.2925
attributes.tag: turbidity method
attributes.value: Turner Cyclops 7 turb