title: Sample 2
organism: Homo sapiens
description: Protocols: To induce cartilaginous differentiation of MPS IH and HD BMSCs the micro-mass culture system was used as previously described (Serafini et al 2014). Pellets were snap frozen Pellets were thawed and homogenized by Mikro-Dismembrator U (Gottingen). Total RNA was extracted using TRIzol reagent (Invitrogen), according to manufacturer&#39;s protocol. Whole transcriptome RNA-seq analyses have been performed by Next Generation Sequencing (NGS), using the Universal RNAseq kit (Nugen, Tecan).The Universal RNA-Seq is an end-to-end solution for strand-specific RNA-Seq library construction used 250 ng nanogram amounts of total RNA obtained from gut sections. The workflow consists of double-stranded cDNA generation using a mixture of random priming, optional fragmentation of double-stranded cDNA, end repair, adaptor ligation, strand selection, targeted transcript depletion with AnyDeplete for globin genes, and PCR amplification to produce the final library. The yields of final libraries were assessed by Qubit 4.0 fluorimeter and their sizing by Agilent Bioanalyzer.
attributes.tag: ENA first public
attributes.value: 2024-06-24
attributes.tag: External Id
attributes.value: SAMEA113956989
attributes.tag: INSDC center name
attributes.value: Tettamanti Center, Fondazione IRCCS San Gerardo dei Tintori
attributes.tag: INSDC last update
attributes.value: 2023-06-29T08:15:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13129:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: chondrocyte
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: postnatal
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: mesenchymal stem cell of the bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-13129:Sample 2
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male