title: EMOBON_BPNS_So_6
organism: marine sediment metagenome
description: EMOBON metagenome sediment sample from station BPNS collected on 2021-07-26 community collected micro
attributes.tag: ENA first public
attributes.value: 2024-11-11
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00085
attributes.tag: broad-scale environmental context
attributes.value: marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
attributes.tag: collection date
attributes.value: 2021-07-26
attributes.tag: depth
attributes.value: 22.94
attributes.tag: dna concentration
attributes.value: 5.33
attributes.tag: elevation
attributes.value: -22.94
attributes.tag: environmental medium
attributes.value: sediment [ENVO:00002007]
attributes.tag: geographic location (country and/or sea)
attributes.value: Belgium
attributes.tag: geographic location (latitude)
attributes.value: 51.433331
attributes.tag: geographic location (locality)
attributes.value: Belgian part of the North Sea
attributes.tag: geographic location (longitude)
attributes.value: 2.808331
attributes.tag: geographic location (ocean)
attributes.value: North Atlantic Ocean
attributes.tag: geographic location (region and locality)
attributes.value: North Sea
attributes.tag: library reads sequenced
attributes.value: 43017431.0
attributes.tag: local environmental context
attributes.value: shallow_marine_sediment_[ENVO:03000034]
attributes.tag: mock_pr
attributes.value: DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
attributes.tag: obs_id
attributes.value: BPNS
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: relevant standard operating procedures
attributes.value: Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
attributes.tag: run
attributes.value: 220712_JARVIS_HMNJKDSX3
attributes.tag: sample collection device
attributes.value: Van Veen Grab
attributes.tag: sample collection method
attributes.value: SoSOP3
attributes.tag: sample material processing
attributes.value: SoSOP3
attributes.tag: sample name
attributes.value: EMOBON00085
attributes.tag: sample storage location
attributes.value: MSO LifeWatch Lab -80 Freezer
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: marine sediment metagenome
attributes.tag: sea surface temperature method
attributes.value: no CTD taken
attributes.tag: sediment temperature method
attributes.value: no CTD taken
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch1
attributes.tag: sequence_batch_rundate
attributes.value: Batch1_220712
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: source material identifiers
attributes.value: EMOBON_BPNS_So_210726_micro_2
attributes.tag: status
attributes.value: sample
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage
attributes.value: high
attributes.tag: tidal stage information
attributes.value: flood_tide
attributes.tag: total depth of water column
attributes.value: 26