title: BC4
organism: Homo sapiens
description: Protocols: PBMCs separation was performed using Ficoll-Paque (Ficoll Plaque PLUS – GE Healthcare Life Sciences, Piscataway, USA) RNA was extracted using TRIzol reagent (TRIzol Reagent, Invitrogen Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions Indexed libraries were prepared from 500ng/ea purified RNA with Illumina Stranded mRNA (Illumina) Library Prep Kit according to the manufacturer's instructions. For library quantifications, TapeStation 4200 (Agilent Technologies) and Qubit (Thermo Fischer) was used.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-30T00:24:23Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-30T00:24:23Z
attributes.tag: External Id
attributes.value: SAMEA112868472
attributes.tag: INSDC center alias
attributes.value: Genomix4Life SRL
attributes.tag: INSDC center name
attributes.value: Genomix4Life SRL
attributes.tag: INSDC first public
attributes.value: 2023-06-30T00:24:23Z
attributes.tag: INSDC last update
attributes.value: 2023-06-30T00:24:23Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12828:BC4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: mononuclear cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: urinary bladder cancer
attributes.tag: individual
attributes.value: 4
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-12828:BC4
attributes.tag: scientific_name
attributes.value: Homo sapiens