title: Sample 2
organism: Hordeum vulgare
description: Protocols: For RNA seq analyses, plant tissue (2 cm long fragments of the second leaf located 3 cm below leaf tip) was collected in four biological replicates, each containing fragments from 4 seedlings of control and drought-stressed plants of both genotypes [here only drought-treated samples are deposited]. Tissue was immediately frozen in liquid nitrogen and stored in -80oC degrees Plants were grown in a greenhouse for 10 days after sowing (DAS) under optimal water conditions (14% vwc), 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity, in boxes (400 × 140 × 175 mm) filled with soil containing a mixture of sandy loam and sand (7:2) supplied with a nutrient medium. On 15 DAS, plants were moved into a growth chamber, where the temperature regime was set to 25°C/20°C day/night, with a 16/8 hr photoperiod and 420 μEm−2 s−1 light intensity RNA was extracted with the use of miRvana kit with phenol according to manufacturer's protocol cDNA libraries were prepared following Illumina TruSeq standard procedures and eventually sequenced in an Illumina NovaSeq6000 sequencer, producing 2x150 bp paired-end reads.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-19
attributes.tag: External Id
attributes.value: SAMEA112845432
attributes.tag: INSDC center alias
attributes.value: Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice
attributes.tag: INSDC center name
attributes.value: Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice
attributes.tag: INSDC first public
attributes.value: 2023-06-19T16:00:42Z
attributes.tag: INSDC last update
attributes.value: 2023-06-19T16:00:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12804:Sample 2
attributes.tag: age
attributes.value: 25
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: Sebastian
attributes.tag: developmental stage
attributes.value: seedling development stage
attributes.tag: genotype
attributes.value: hvd14
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-12804:Sample 2
attributes.tag: scientific_name
attributes.value: Hordeum vulgare