title: ZNF280D
organism: synthetic construct
description: Protocols: Input libraries were sequenced without any filtering. Eluted libraries (data from experiments) underwent selection based on TFs used in the SMILE-seq procedure. DNA libraries were collected after the SMiLE-seq procedure, purified and amplified via PCR. The amplified libraries were size selected using AMPureXP beads and amplified with i5 and i7 Nextera adapters to prepare for sequencing. Libraries were ordered as hand-mixed DNA oligos from IDT and synthesized via enzymatic reaction using DNA Polymerase I (Large Klenow Fragment).
attributes.tag: ENA first public
attributes.value: 2024-11-11
attributes.tag: INSDC center name
attributes.value: École Polytechnique Fédérale de Lausanne (EPFL)
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14598:ZNF280D
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sample name
attributes.value: E-MTAB-14598:ZNF280D
attributes.tag: scientific_name
attributes.value: synthetic construct