title: Kidney organoid, iPSC derived, rep1
organism: Homo sapiens
description: Cryopreserved and thawed NPC aggregates, day 11 IPCs and ND cells were dissociated into single cells by incubation with Accumax and gentle pipetting. The dissociated single cells were mixed, resuspended in the organoid medium and seeded into 96-well low cell-binding U-bottom plates at a density of 1.7*10^4 NPCs/well, 1.7*10^4 IPCs/well and 1.7*10^4 ND cells/well to form aggregates. After 48 h, the mixed aggregates were transferred to a 24-well plate and incubated with 20 uL of 50% Matrigel in the same medium. After solidification to some extent, 180 uL of the same medium was added. Then, the medium was replaced with half the volume of fresh basal medium every two days.
attributes.tag: sample_name
attributes.value: Kidney_organoid1
attributes.tag: age
attributes.value: not applicable
attributes.tag: biomaterial_provider
attributes.value: Shinya Yamanaka
attributes.tag: cell_type
attributes.value: hiPS cell derived kidney organoids
attributes.tag: collection_date
attributes.value: 2023-07-04
attributes.tag: geo_loc_name
attributes.value: Japan
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sex
attributes.value: not applicable
attributes.tag: tissue
attributes.value: not applicable
attributes.tag: BioSampleModel
attributes.value: Human