title: Chromium_lung
organism: Homo sapiens
description: Protocols: Curls with 2x25 micrometers for lung, breast and DLBCL were sectioned from FFPE blocks. Nuclei were extracted using snPATHO protocol9, based on the protocol from 10X (CG000632). The nuclei were counted on the LUNA-FX7 cell counter (AO/PI viability kit, Logos). Pools of four samples were processed together as shown in Supplementary Table 4, targeting 40,000 nuclei recovery per pool (10,000 nuclei per sample). Replicas for two donors B3 and B4 were processed independently. The protocol was followed by the manufacturer's protocol Chromium Fixed RNA profiling - Multiplexed samples (CG000527).
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: disease
attributes.value: lung cancer
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: collection date
attributes.value: not collected
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: common name
attributes.value: human
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected