title: H4_S104
organism: Mus musculus
description: Protocols: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described. 100 myHSC or 100 balHSC were sorted into 1.7ml tubes with the reaction buffer (water, lysis buffer, and RNase inhibitor) and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-04
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-04
attributes.tag: External Id
attributes.value: SAMEA113402311
attributes.tag: INSDC center alias
attributes.value: Fred Hutchinson Cancer Center
attributes.tag: INSDC center name
attributes.value: Fred Hutchinson Cancer Center
attributes.tag: INSDC first public
attributes.value: 2023-06-04T00:15:42Z
attributes.tag: INSDC last update
attributes.value: 2023-06-04T00:15:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12890:H4_S104
attributes.tag: age
attributes.value: 8 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: hematopoietic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: baseline-4
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-12890:H4_S104
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J