title: No_163
organism: Mus musculus
description: Protocols: After removing the medium, organoids were washed 3 x with 1ml PBS w/o Ca++/Mg++ and then collected for RNA extraction. Total RNA was isolated using the PeqGold microspin total RNA Kit following manufacturers' instructions (Peqlab/VWR). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-07-01T00:20:33Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-07-01T00:20:33Z
attributes.tag: External Id
attributes.value: SAMEA112655566
attributes.tag: INSDC center alias
attributes.value: Department of Medicine 1, Friedrich Alexander University, Erlangen-Nuremberg, Germany
attributes.tag: INSDC center name
attributes.value: Department of Medicine 1, Friedrich Alexander University, Erlangen-Nuremberg, Germany
attributes.tag: INSDC first public
attributes.value: 2023-07-01T00:20:33Z
attributes.tag: INSDC last update
attributes.value: 2023-07-01T00:20:33Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12662:No_163
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Stat1 KO Stat2 KO
attributes.tag: growth condition
attributes.value: intestinal organoid
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: small intestine
attributes.tag: sample name
attributes.value: E-MTAB-12662:No_163
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6