title: LigneesW-neg-T2
organism: Mus musculus
description: Protocols: Grafted breast tumor tissue was collected as described previously (doi: 10.15252/emmm.202013270). Tumor samples were fresh frozen Total RNA was extracted using RNeasy® Plus Micro Kit (Qiagen) according to the manufacturer's instructions. Libraries were prepared from RNA using SMARTer Stranded Total RNA-Seq Kit, Pico Input Mammalian (Takara) following the manufacturer's instructions. Briefly, random primers were used for first-strand synthesis, and ribosomal cDNA was cleaved by ZapR V.2 in the presence of mammalian R-probes V.2.
attributes.tag: strain
attributes.value: FVB
attributes.tag: RNA interference
attributes.value: sh TRAIL
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: female
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: common name
attributes.value: house mouse
attributes.tag: organism part
attributes.value: Tumor
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Tenascin KO
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: age
attributes.value: 3
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected