title: 71M
organism: Homo sapiens
description: Protocols: Renal orthotopic implantation was carried out by injection of 1.106 786-O luc or ACHN luc cells into the left kidney of athymic nude mice. Tumor growth was followed by intraperitoneal injection of 150 mg/kg D-luciferin Potassium salt using IVIS Imager (Perkin Elmer). For tumoroid generation, cell dissociation was performed thanks to the gentleMACS Dissociator and the Tumor dissociation kit (Miltenyl Biotec). Dissociated cells were counted and coated with magnetic NanoShuttle (Greiner) (1 µL per 20 000 cells). Tumoroids were cultured for 14 days in DMEM media supplemented with 10 % FBS (GIBCO), 100 U/mL penicillin + streptomycin (Invitrogen). Total RNA was extracted from treated cells using the MirVana PARIS kit (Thermofisher). The 3′ Bulk RNA Barcoding and sequencing (BRB-seq) experiments were performed at the Research Institute for Environmental and Occupational Health (Irset, Rennes, France) according to the published protocol (Alpern et al., 2019).The sequencing libraries were built by tagmentation using 50 ng of ds cDNA with Illumina Nextera XT Kit (Illumina, #FC-131-1024)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-30T00:24:27Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-30T00:24:27Z
attributes.tag: External Id
attributes.value: SAMEA113425362
attributes.tag: INSDC center alias
attributes.value: Biosante UMR 1292 Health Department (DS) Interdisciplinary Research Institute of Grenoble (IRIG)
attributes.tag: INSDC center name
attributes.value: Biosante UMR 1292 Health Department (DS) Interdisciplinary Research Institute of Grenoble (IRIG)
attributes.tag: INSDC first public
attributes.value: 2023-06-30T00:24:27Z
attributes.tag: INSDC last update
attributes.value: 2023-06-30T00:24:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12928:71M
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: 786-O
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: renal cell carcinoma
attributes.tag: organism part
attributes.value: kidney
attributes.tag: sample name
attributes.value: E-MTAB-12928:71M
attributes.tag: sampling site
attributes.value: neoplasm
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: not available
attributes.tag: xenograft
attributes.value: 786-O cells implanted in kidney of athymic nude mice