title: Sample 1
organism: Malus domestica
description: Protocols: RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. Truseq stranded mRNA Assay. Paired end.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-12
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-12
attributes.tag: External Id
attributes.value: SAMEA12813419
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC first public
attributes.value: 2023-01-12T00:17:10Z
attributes.tag: INSDC last update
attributes.value: 2023-01-12T00:17:10Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11410:Sample 1
attributes.tag: age
attributes.value: 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: apple
attributes.tag: cultivar
attributes.value: Lord Lambourne
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: stem
attributes.tag: sample name
attributes.value: E-MTAB-11410:Sample 1
attributes.tag: scientific_name
attributes.value: Malus domestica