title: MY73-OE_rep2
organism: Arabidopsis thaliana
description: Protocols: Total RNA were extracted from pooled Arabidopsis siliques 12 days after flowering (DAF). Arabidopsis thaliana seedlings are grown chambers under constrant temperature of 23°C and a photoperiod of 16 h light (100‐150 μmol m−2 sec−1 354 illumination)/8 h dark. Total RNA extraction was carried out using the Monarch Total RNA Miniprep Kit (New England Biolabs), following the manufacturer's protocols. Subsequently, DNase I (New England Biolabs) was employed to remove genomic DNA, according to the manufacturer's instructions. RNA-seq libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer's instruction
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Arabidopsis thaliana
attributes.tag: organism
attributes.value: Arabidopsis thaliana
attributes.tag: collection date
attributes.value: not collected
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana
attributes.tag: common name
attributes.value: thale cress
attributes.tag: organism part
attributes.value: Siliques
attributes.tag: age
attributes.value: 12 Days After Flowering (DAF)
attributes.tag: genotype
attributes.value: MYB73-OE
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected