title: DMSO
organism: Homo sapiens
description: Protocols: 786-O cells were grown in RPMI medium containting 10% FCS for 24h before treatment. 786-O cells (150, 000) were treated for 24h with 0.1% DMSO or 5 µM of CX4945 or AB668. ccRCC cell lines 786-O was obtained from ATCC. Total RNA was extracted from treated cells using the MirVana PARIS kit (Thermofisher). The 3′ Bulk RNA Barcoding and sequencing (BRB-seq) experiments were performed at the Research Institute for Environmental and Occupational Health (Irset, Rennes, France) according to the published protocol (Alpern et al., 2019).The sequencing libraries were built by tagmentation using 50 ng of ds cDNA with Illumina Nextera XT Kit (Illumina, #FC-131-1024)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-05-27
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-05-27
attributes.tag: External Id
attributes.value: SAMEA112795193
attributes.tag: INSDC center alias
attributes.value: Biosante UMR 1292 Health Department (DS) Interdisciplinary Research Institute of Grenoble (IRIG)
attributes.tag: INSDC center name
attributes.value: Biosante UMR 1292 Health Department (DS) Interdisciplinary Research Institute of Grenoble (IRIG)
attributes.tag: INSDC first public
attributes.value: 2023-05-27T00:18:42Z
attributes.tag: INSDC last update
attributes.value: 2023-05-27T00:18:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12776:DMSO
attributes.tag: age
attributes.value: 58
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: 786-O
attributes.tag: cell_type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: renal cell carcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: sample name
attributes.value: E-MTAB-12776:DMSO
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male