title: mus_SNuc7503536
organism: Mus musculus
description: Protocols: Three 19 months old and five 3 months old male mice of C57BL/6JRj strain were obtained from Janvier labs, France.They were used to dissect hindlimb muscles for the single-nucleus isolation according to the following protocol: https://www.protocols.io/view/nuclei-isolation-from-human-skeletal-muscle-kqdg34pqel25/v1. Briefly, muscle tissue was grounded using a dounce homogenizer, lysed in the nuclei lysis buffer and, finally, Percoll gradient was used to separate intact nuclei for loading and cell debris. Cell or nuclei suspension were  loaded on the Chromium Controller (10X Genomics) with a targeted cell or nuclei recovery of 8000.  10X Genomics Single Cell 3â€™ v2 or v3 Reagent Kit was used to create GEM, perform cDNA synthesis and generate sequencing libraries according to standard instructions.
attributes.tag: individual
attributes.value: Mouse2
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: male
attributes.tag: cohort
attributes.value: young
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: common name
attributes.value: house mouse
attributes.tag: organism part
attributes.value: hindlimb muscle
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: injury
attributes.value: normal
attributes.tag: cell_type
attributes.value: nuclei
attributes.tag: age
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected