title: Potter Cove sediment slurry SIP incubation - 12C Acetate.Sulfate at 10 degrees C - RNA Ultra-light fraction
organism: marine sediment metagenome
description: Potter Cove sediment slurry SIP incubation with unlabeled acetate and sulfate at 10 degree Celsius, density separated RNA Ultra-light fraction (= gradient position 5), sediment from station 13, core STA13.04, Potter Cove, Antarctica; parameters geographic location, depth and collection date  refer to sediment used to prepare the slurry; parameter original_fraction refers to pooled fraction numbers of total fractions, gradient_pos_density and gradient_pos_rel_amt refer to calculated average of pooled fractions; for more information about slurry sample refer to BioSample submitted under accession number given under source_mat_ID
attributes.tag: ENA first public
attributes.value: 2024-11-11
attributes.tag: INSDC center name
attributes.value: UniBremen
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 10degrees.12C.Acetate.Sulfate.Fraction.Ultra-light
attributes.tag: broad-scale environmental context
attributes.value: shallow marine sediment [ENVO:03000034] | polar biom [ENVO:01000339] | glacial retreat [ENVO:01001656] | coast [ENVO:01000687]
attributes.tag: chem_administration
attributes.value: acetate, sulfate
attributes.tag: collection date
attributes.value: 2019-01-23
attributes.tag: depth
attributes.value: 0 - 0.29
attributes.tag: elevation
attributes.value: -15
attributes.tag: environmental medium
attributes.value: microbial community [PCO:1000004] | mesocosm [ENVO:01000620]
attributes.tag: experimental factor
attributes.value: different substrate additions | RNA stable isotope probing | exposure temperature [EFO:0000718] | response to heat [GO:0009408] | labelling [EFO:0000562]
attributes.tag: geographic location (country and/or sea)
attributes.value: Antarctica
attributes.tag: geographic location (latitude)
attributes.value: -62.2253889
attributes.tag: geographic location (longitude)
attributes.value: -58.6411667
attributes.tag: geographic location (region and locality)
attributes.value: King George Island/Isla 25 de Mayo, Potter Cove
attributes.tag: gradient_pos_density
attributes.value: 1.7815
attributes.tag: gradient_pos_rel_amt
attributes.value: 0.03995
attributes.tag: gradient_position
attributes.value: 5
attributes.tag: internal_standard
attributes.value: 10.1007/978-1-4939-9721-3_15
attributes.tag: isotope
attributes.value: 13C
attributes.tag: isotopolog
attributes.value: 175
attributes.tag: isotopolog_approach
attributes.value: single
attributes.tag: isotopolog_atom_frac
attributes.value: 0.011
attributes.tag: isotopolog_atom_pos
attributes.value: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1/i1+1,2+1;
attributes.tag: isotopolog_dose
attributes.value: 30.026
attributes.tag: isotopolog_incu_time
attributes.value: T264:00
attributes.tag: isotopolog_label
attributes.value: natural abundance
attributes.tag: local environmental context
attributes.value: anaerobic sediment [ENVO:00002045]
attributes.tag: original_fraction
attributes.value: 11+12/14
attributes.tag: project name
attributes.value: Bacterial 16S rRNA community in acetate degrading stable isotope probing slurry incubation experiment across temperature range with sediment of Potter Cove
attributes.tag: relationship to oxygen
attributes.value: anaerobe
attributes.tag: sample name
attributes.value: 10degrees.12C.Acetate.Sulfate.Fraction.Ultra-light
attributes.tag: scientific_name
attributes.value: marine sediment metagenome
attributes.tag: sip_method
attributes.value: 10.1007/978-1-4939-9721-3_15
attributes.tag: source_mat_alias
attributes.value: tempSIP_17
attributes.tag: source_mat_id
attributes.value: ERS22115424