title: Total RNA of CHO-S cells without PBS washing
organism: Cricetulus griseus
description: Total RNA from CHO-S cells (Thermo Fisher Scientific, FreeStyle CHO-S Cells: R80007) was extracted using RNeasy (QIAGEN) following manufactures' protocol without washing the harvested cells by PBS buffer.
attributes.tag: sample_name
attributes.value: CHO-S_RNA_nopbs
attributes.tag: age
attributes.value: Pasage 15
attributes.tag: cell_line
attributes.value: CHO-S
attributes.tag: cell_type
attributes.value: Suspension cells
attributes.tag: collection_date
attributes.value: 2022-12-01
attributes.tag: dev_stage
attributes.value: CHO-S_RNA
attributes.tag: geo_loc_name
attributes.value: Japan:Osaka
attributes.tag: growth_protocol
attributes.value: CHO-S cells were grown in 20 mL of FreeStyle CHO Expression Medium (14365C-1000ML; Gibco, Grand Island, NY, USA) containing 600 L of 200mmol/L L-Glutamine Solution (x100) (073-05391, Fujifilm Wako Pure Chemical, Osaka, Japan) in a 125 mL flask. The flask was shaken (140 rpm) at 37 degree C in a humidified 5% CO2 atmosphere.
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: CHO-S
attributes.tag: tissue
attributes.value: cell