title: HCC827_ctrl_C
organism: Homo sapiens
description: Protocols: Cells were plated at 6cm cells and 24 hours later transfected with control or RBM10 siRNA. 48 hours after siRNA transfection, cells were directly lysed using TRIzol reagent (Ambion) for RNA extraction. Cells were grown in a humidified 37C incubator (5% CO2) in RPMI-1640 medium (Gibco) supplemented with 10% heat-inactivated FBS, 2mM L-glutamine (Gibco), 100unit/mL penicillin and 100μg/mL streptomycin (Gibco). siRNA transfections were performed using Lipofectamine™ RNAiMAX (Invitrogen) according to the manufacturer's instructions. Total RNA was extracted from cells using TRIzol reagent according to the manufacturer's instructions (Ambion). RNA-seq libraries were prepared at the Crown Genomics institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science using TruSeq mRNA library preparation kit (Illumina). Libraries were quantified by Qubit (Thermo fisher scientific) and TapeStation (Agilent).
attributes.tag: ENA first public
attributes.value: 2024-04-01
attributes.tag: External Id
attributes.value: SAMEA114857225
attributes.tag: INSDC center name
attributes.value: Faculty of Biology - Technion Israel Institute of Technology
attributes.tag: INSDC last update
attributes.value: 2023-12-07T22:46:10Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13617:HCC827_ctrl_C
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: HCC827
attributes.tag: cell_type
attributes.value: epithelial cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: lung adenocarcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-13617:HCC827_ctrl_C
attributes.tag: scientific_name
attributes.value: Homo sapiens