title: Op_803_1
organism: Caenorhabditis elegans
description: Protocols: Twenty young adult animals were picked to the fresh plates and allowed to lay eggs for 2 hours, after which the adult animals were removed and eggs were allowed to develop into L4 stage. Ten plates of L4 animals were washed off and placed on either OP50 or V. cholerae containing plates for 8 hours. Subsequently, the animals were collected and rinsed three times with M9 buffer. After washing, the worm pellet was frozen in liquid nitrogen. Animals were homogenized using Bullet Blender (Next Advance) in the presence of Qiazol Lysis Reagent and 0.5mm Zirconia beads at 4°C. RNA was prepared using RNeasy Plus Universal Mini Kit (Qiagen). Library was constructed and sequenced by Novogene
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: individual
attributes.value: mixed
attributes.tag: organism
attributes.value: Caenorhabditis elegans
attributes.tag: organism
attributes.value: Caenorhabditis elegans
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: mixed
attributes.tag: scientific_name
attributes.value: Caenorhabditis elegans
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: developmental stage
attributes.value: L4 larva
attributes.tag: genotype
attributes.value: shk-1 KO
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: infect
attributes.value: none