title: Sample 4
organism: Solea senegalensis
description: Protocols: By the end of the experiment, 3 male fish per tank (2 tanks per group) were randomly sacrificed with a lethal dose of anesthesia (1000 ppm phenoxyethanol) and testis were immediately dissected and frozen in liquid nitrogen. Testis samples were stored at -80 ºC for RNA extraction and further RNA-Seq quantification. The gonads collected were homogenized in 1 mL of TRI Reagent® (Sigma-Aldrich, Switzerland) using a tissue-lyzer Star-Beater (VWR). Total RNA was extracted following the instructions of the manufacturer and the obtained pellet was resuspended in RNase-free distilled water (Sigma-Aldrich, Switzerland). Total RNA concentrations were measured with a Nanodrop One-C spectrophotometer (Thermo Fisher Scientific, Washington, USA) and adjusted to 1 µg/µL. Total RNA quality and integrity were checked using a 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA), and only samples with an RNA integrity number (RIN) > 8 were considered for sequencing. A total of 12 libraries were prepared using a TruSeq™ RNA Library Preparation Kit v2 of Illumina (Illumina, USA).
attributes.tag: ENA first public
attributes.value: 2024-08-01
attributes.tag: INSDC center name
attributes.value: Centre of Marine Sciences (CCMAR)
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14218:Sample 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: Senegalese sole
attributes.tag: developmental stage
attributes.value: Breeder
attributes.tag: diet
attributes.value: control
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: 4
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: testis
attributes.tag: sample name
attributes.value: E-MTAB-14218:Sample 4
attributes.tag: scientific_name
attributes.value: Solea senegalensis
attributes.tag: sex
attributes.value: male