title: DMSO2
organism: Homo sapiens
description: Protocols: AGS cells were digested with trypsin, then were neutralized and collected.  Human gastric cancer cell line AGS was cultured in DMEM high-glucose culture medium containing 10%FBS, placed in a 5% CO2 incubator at 37 ℃, digested with 0.25% trypsin every 3 to 4 days, and passed through 1:3 cells with logarithmic growth phase for experiment. AGS cells treated with capsaicin (at a dose of 250uM for 24h) or DMSO Total RNA was collected from AGS cells treated with capsaicin or DMSO using TRIzol reagent according to the manufacturer's protocol (n=3 per group). RNA was quantified using a NanoDrop ND-2000 (Thermo Scientific, USA), and RNA integrity was assessed using an Agilent Bioanalyzer 2100 (Agilent Technologies, USA).  Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.
attributes.tag: disease
attributes.value: gastric carcinoma
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: collection date
attributes.value: not collected
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: stomach
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: cell_line
attributes.value: AGS
attributes.tag: cell_type
attributes.value: epithelial
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected