title: Acute (Figure 6)
organism: Mus musculus
description: Protocols: Peripheral blood (5-6 drops) was collected into 1ml cold RPMI medium containing 5 U/mL heparin by tail vein bleed. Diluted blood (50 μL) was further diluted in 2 mL cold 1% BSA/PBS and RBCs isolated away from leukocytes by cell sorting. RBCs cells were then sorted as single-cells using the Chromium 10X Genomics platform. Approximately 5,000 RBCs (red blood cells), some of which were parasitized, were loaded per channel onto a Chromium controller (10x Genomics) for generation of gel bead-in-emulsions Sequencing libraries were prepared using Single Cell 3' Reagent Kits v3 or where specified v2 (10x Genomics).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-27
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-27
attributes.tag: External Id
attributes.value: SAMEA113476851
attributes.tag: INSDC center alias
attributes.value: Peter Doherty Institute for Infection & Immunity, University of Melbourne
attributes.tag: INSDC center name
attributes.value: Peter Doherty Institute for Infection & Immunity, University of Melbourne
attributes.tag: INSDC first public
attributes.value: 2023-06-27T04:00:06Z
attributes.tag: INSDC last update
attributes.value: 2023-06-27T04:00:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12954:Acute (Figure 6)
attributes.tag: age
attributes.value: 6 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: erythrocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: prior acute Plasmodium berghei ANKA infection
attributes.tag: infect
attributes.value: Plasmodium berghei ANKA
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-12954:Acute (Figure 6)
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J