title: EMOBON_PiEGetxo_Wa_5
organism: metagenome
description: EMOBON metagenome water sample from station PiEGetxo collected on 2021-08-24 size fraction 3-200um
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: Submitter Id
attributes.value: EMOBON00039
attributes.tag: broad-scale environmental context
attributes.value: Control
attributes.tag: collection date
attributes.value: 2021-08-24
attributes.tag: depth
attributes.value: 0
attributes.tag: environmental medium
attributes.value: MilliQ water [ENVO:00003065]
attributes.tag: geographic location (country and/or sea)
attributes.value: missing: control sample
attributes.tag: geographic location (latitude)
attributes.value: missing: control sample
attributes.tag: geographic location (locality)
attributes.value: missing: control sample
attributes.tag: geographic location (longitude)
attributes.value: missing: control sample
attributes.tag: geographic location (ocean)
attributes.value: missing: control sample
attributes.tag: geographic location (region and locality)
attributes.value: missing: control sample
attributes.tag: library reads sequenced
attributes.value: 78378535
attributes.tag: local environmental context
attributes.value: Negative
attributes.tag: mock_eu
attributes.value: DDC_AAABOSDA_4_HMGW5DSX3.UDI213
attributes.tag: mock_pr
attributes.value: DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
attributes.tag: obs_id
attributes.value: PiEGetxo
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:2;Nocycles:18
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: relevant standard operating procedures
attributes.value: Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
attributes.tag: sample material processing
attributes.value: WaSOP 1
attributes.tag: sample name
attributes.value: EMOBON00039
attributes.tag: sample storage location
attributes.value: BBEBB, freezer 12
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: metagenome
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch1
attributes.tag: sequence_batch_rundate
attributes.value: Batch1_220622
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: size-fraction lower threshold
attributes.value: 3
attributes.tag: size-fraction upper threshold
attributes.value: 200
attributes.tag: source material identifiers
attributes.value: EMOBON_PiEGetxo_Wa_210824_3um_blank
attributes.tag: status
attributes.value: blank
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage information
attributes.value: no_tide
attributes.tag: total depth of water column
attributes.value: 10