title: EMOBON_NRMCB_So_7
organism: marine sediment metagenome
description: EMOBON metagenome sediment sample from station NRMCB collected on 2021-08-31 community collected micro
attributes.tag: ENA first public
attributes.value: 2024-11-11
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00098
attributes.tag: broad-scale environmental context
attributes.value: marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
attributes.tag: collection date
attributes.value: 2021-08-31
attributes.tag: depth
attributes.value: 75
attributes.tag: dissolved oxygen
attributes.value: 4.7075
attributes.tag: dissolved oxygen method
attributes.value: SBE43
attributes.tag: dna concentration
attributes.value: 33.3
attributes.tag: elevation
attributes.value: -75
attributes.tag: environmental medium
attributes.value: sediment [ENVO:00002007]
attributes.tag: geographic location (country and/or sea)
attributes.value: Italy
attributes.tag: geographic location (latitude)
attributes.value: 40.800139
attributes.tag: geographic location (locality)
attributes.value: Naples Gulf
attributes.tag: geographic location (longitude)
attributes.value: 14.25
attributes.tag: geographic location (ocean)
attributes.value: Mediterranean Sea - Western Basin
attributes.tag: geographic location (region and locality)
attributes.value: Tyrrhenian Sea
attributes.tag: library reads sequenced
attributes.value: 50894999
attributes.tag: local environmental context
attributes.value: shallow_marine_sediment_[ENVO:03000034]
attributes.tag: mock_pr
attributes.value: DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
attributes.tag: obs_id
attributes.value: NRMCB
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: pigments
attributes.value: 0.6438
attributes.tag: pigments method
attributes.value: this is not pigments but fluorescense. chl Turner Design Cyclops 7C)
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: relevant standard operating procedures
attributes.value: Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
attributes.tag: salinity
attributes.value: 37.9887
attributes.tag: salinity method
attributes.value: CTD SBE911plusV2
attributes.tag: sample collection device
attributes.value: sediment corer
attributes.tag: sample material processing
attributes.value: So SOP1
attributes.tag: sample name
attributes.value: EMOBON00098
attributes.tag: sample storage location
attributes.value: freezer #1 BEOM; upper compartment; rack #8 d III (see scheme in technical support EMI)
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: marine sediment metagenome
attributes.tag: sea surface temperature
attributes.value: 26.8119
attributes.tag: sea surface temperature method
attributes.value: CTD SBE911plusV2
attributes.tag: sediment temperature
attributes.value: 14.9343
attributes.tag: sediment temperature method
attributes.value: CTD SBE911plusV2
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch1
attributes.tag: sequence_batch_rundate
attributes.value: Batch1_220712
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: source material identifiers
attributes.value: EMOBON_NRMCB_So_210831_micro_2
attributes.tag: status
attributes.value: sample
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage information
attributes.value: no_tide
attributes.tag: total depth of water column
attributes.value: 75