title: NAD+ resistent clone 3
organism: Streptococcus pneumoniae
description: Protocols: Streptococcus pneumoniae was cultivated in liquid medium with increasing concentrations (50 µM to 5 mM) of NAD+. After six passages, bacteria were plated on blood agar supplemented with 500 µM NAD+ and three clones were picked Total DNA for resequencing was extracted using a DNeasy Blood and Tissue kit (Qiagen) according to the manual. DNA libraries for sequencing were generated by applying a Nextera XT DNA Library Preparation kit (Illumina)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-12
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-12
attributes.tag: External Id
attributes.value: SAMEA112652323
attributes.tag: INSDC center alias
attributes.value: Center for synthetic microbiology SYNMIKRO Philipps University Marburg
attributes.tag: INSDC center name
attributes.value: Center for synthetic microbiology SYNMIKRO Philipps University Marburg
attributes.tag: INSDC first public
attributes.value: 2023-06-12T17:00:05Z
attributes.tag: INSDC last update
attributes.value: 2023-06-12T17:00:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12644:NAD+ resistent clone 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: spontaneus NAD+ resistent clone
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-12644:NAD+ resistent clone 3
attributes.tag: scientific_name
attributes.value: Streptococcus pneumoniae
attributes.tag: strain
attributes.value: D39