title: WTD2
organism: Hordeum vulgare
description: Protocols: For RNA seq analyses, plant tissue (2 cm long fragments of the second leaf located 3 cm below leaf tip) was collected in four biological replicates, each containing fragments from 4 seedlings of control and drought-stressed plants of both genotypes [here only drought-treated samples are deposited]. Tissue was immediately frozen in liquid nitrogen and stored in -80oC degrees Drought stress was applied according to the previously described protocol (Daszkowska-Golec et al., 2019). Briefly, plants were grown in a greenhouse for 10 days after sowing (DAS) under optimal water conditions (14% vwc), 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity, in boxes (400 × 140 × 175 mm) filled with soil containing a mixture of sandy loam and sand (7:2) supplied with a nutrient medium. Next, the soil moisture was decreased by withholding the irrigation. On 15 DAS, the soil moisture decreased to 3%, and plants were moved into a growth chamber, where the temperature regime was set to 25°C/20°C day/night, with a 16/8 hr photoperiod and 420 μEm−2 s−1 light intensity (Figure 1A). The severe drought stress (3–1.5% vwc) was applied for the next 10 days (16–25 DAS). The control plants were grown under the same conditions with optimal water supply (14% vwc) parallel to the drought-treated plants. The soil moisture was measured daily using a Time-domain reflectometer EasyTest (Institute of Agrophysics, Polish Academy of Sciences, Poland). Drought stress was applied according to the previously described protocol (Daszkowska-Golec et al., 2019). Briefly, plants were grown in a greenhouse for 10 days after sowing (DAS) under optimal water conditions (14% vwc), 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity, in boxes (400 × 140 × 175 mm) filled with soil containing a mixture of sandy loam and sand (7:2) supplied with a nutrient medium. Next, the soil moisture was decreased by withholding the irrigation. On 15 DAS, the soil moisture decreased to 3%, and plants were moved into a growth chamber, where the temperature regime was set to 25°C/20°C day/night, with a 16/8 hr photoperiod and 420 μEm−2 s−1 light intensity (Figure 1A). The severe drought stress (3–1.5% vwc) was applied for the next 10 days (16–25 DAS). The control plants were grown under the same conditions with optimal water supply (14% vwc) parallel to the drought-treated plants. The soil moisture was measured daily using a Time-domain reflectometer EasyTest (Institute of Agrophysics, Polish Academy of Sciences, Poland). RNA was extracted with the use of miRvana kit with phenol according to manufacturer's protocol cDNA libraries were prepared following Illumina TruSeq standard procedures and eventually
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-19
attributes.tag: External Id
attributes.value: SAMEA112824066
attributes.tag: INSDC center alias
attributes.value: Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice
attributes.tag: INSDC center name
attributes.value: Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice
attributes.tag: INSDC first public
attributes.value: 2023-06-19T20:23:50Z
attributes.tag: INSDC last update
attributes.value: 2023-06-19T20:23:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12796:WTD2
attributes.tag: age
attributes.value: 25
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: Sebastian
attributes.tag: developmental stage
attributes.value: seedling development stage
attributes.tag: envrionmental stress
attributes.value: drought environment
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-12796:WTD2
attributes.tag: scientific_name
attributes.value: Hordeum vulgare