title: CTRL1
organism: Homo sapiens
description: Protocols: This case-control study consists of a mRNA analysis on peripheral blood mononuclear cells (PBMCs) performed by RNA-seq in 25 participants of Sicilian ancestry: 12  patients and 13 age- and sex-matched healthy controls, all recruited at the Oasi Research InstituteIRCCS of Troina (Italy). Total RNA was extracted using Zymo Research, according to the manufacturer's instructions. The RNA was transcribed to cDNA using oligo (dT) primers and the GeneAmp RNA PCR Core Kit (Applied Biosystems) and the cDNA was amplified via real-time PCR using Power- SYBR Green PCR Master Mix (Promega) on the 7500 Fast Real-Time PCR System (Applied Biosystems). RNA concentration and purity were evaluated using NanoDrop™ 2000/2000c (Thermo Fisher Scientific, Waltham, MA, USA), whereas sample integrity was analyzed using Tape Station 4200 (Agilent Technologies, Santa Clara, CA, USA) using an RNA Screen Tape Assay. Indexed libraries were prepared from 1 µg/ea purified RNA using the TruSeq Stranded mRNA (Illumina, San Diego, CA, USA) Library Prep Kit according to the manufacturer's instructions. After the enrichment of mRNA using oligo dT magnetic beads and fragmentation, cDNA synthesis was performed, followed by adapter ligation and PCR amplification. For library quantifications, TapeStation 4200 (Agilent Technologies) was used. Indexed libraries were pooled in equimolar amounts, with a final concentration of 1.5 nM.
attributes.tag: ENA first public
attributes.value: 2024-09-09
attributes.tag: INSDC center name
attributes.value: Genomix4life s.r.l
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13871:CTRL1
attributes.tag: age
attributes.value: 17
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: peripheral blood mononuclear cells (PBMCs)
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: control
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: 1
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-13871:CTRL1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male