title: Metabolic gene WPS series met2-lib5_redo
organism: Caenorhabditis elegans
description: Multiplexed RNA-seq library generated by Worm Perturb-seq (WPS) technology. Contains the triplicate samples for ~16 RNAi conditions targeting different metabolic genes and six samples from empty vector control treatment. To analyze the data, refer to our publication/GEO session for the metadata table and WPS data processing pipeline.
attributes.tag: strain
attributes.value: N2
attributes.tag: dev_stage
attributes.value: Gravid adult (63-66 hours post L1 seeding)
attributes.tag: collection_date
attributes.value: 2020-08-25/2020-09-09
attributes.tag: geo_loc_name
attributes.value: USA: Worcester, Massachusetts
attributes.tag: sex
attributes.value: hermaphrodite
attributes.tag: tissue
attributes.value: Whole animal (excluding eggs)
attributes.tag: biomaterial_provider
attributes.value: Marian Walhout Lab, University of Massachusetts Chan Medical School. Address: 368 Plantation Street, Albert Sherman Center, Worcester, MA 01605, USA
attributes.tag: collected_by
attributes.value: Hefei Zhang, University of Massachusetts Chan Medical School
attributes.tag: sample_type
attributes.value: Whole organism
attributes.tag: store_cond
attributes.value: fresh sample
attributes.tag: treatment
attributes.value: Including empty vector controls and single gene RNAi knockdown for genes: C56G3.2, NOSIGNAL-acs-3, SHORT-cka-2, acl-2, cpt-2, K07B1.4, C27A7.5, T03G6.3, hphd-1, T12B3.3, F09G2.8, pld-1, SHORT-acl-8, acs-2, unc-17. [Note: this is from the experiment where we redid library construction for part of the samples in met2-lib5, using the original total RNA extraction. These samples correspond to one biological replicate that was missed in met2-lib5 because of a pipetting error.]
attributes.tag: BioSampleModel
attributes.value: Model organism or animal