title: wildtype-0h
organism: Homo sapiens
description: Protocols: Wildtype, CDK2-knockdown and JUN-knockdown THP1 cells were treated with 100 ng/ml LPS for 3 hours. Control group wildtype THP1 cells were untreated. Total RNA was isolated from cultured cells using the E.Z.N.A.® HP Total RNA Kit (Omega Bio Tek) according to manufacturer's instructions. An Illumina TruSeq Stranded mRNA Library prep kit was used to generate the RNA libraries. The library quantity was measured using the picogreen method.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-07-05
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-07-05
attributes.tag: External Id
attributes.value: SAMEA110180651
attributes.tag: INSDC center alias
attributes.value: UTSW
attributes.tag: INSDC center name
attributes.value: UT Southwestern Medical Center
attributes.tag: INSDC first public
attributes.value: 2022-07-05T12:04:00Z
attributes.tag: INSDC last update
attributes.value: 2022-07-05T12:04:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11872:wildtype-0h
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: THP-1
attributes.tag: cell_type
attributes.value: monocyte
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: acute monocytic leukemia
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-11872:wildtype-0h