title: NG-6612_R1_dnCad_lib21093_1481_2
organism: Paracentrotus lividus
description: Protocols: Two independent batches of embryos were used for each condition. Embryos with DN-cadherin mRNA were collected at the early blastula stage (6h30 after fertilization at 18°C). RNA was purified using Trizol. Libraries were made by the GATC company. Briefly, polyadenylated transcripts were purified by two rounds of oligo-dT chromatography and cDNA libraries were constructed using a Superscript double stranded cDNA synthesis kit (Invitrogen), fragmented by sonication, and cDNA fragments of 200-500 bp were gel purified. Illumina adaptaters were ligated to these fragments.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-16
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-16
attributes.tag: External Id
attributes.value: SAMEA113426895
attributes.tag: INSDC center alias
attributes.value: Institut de Biologie Valrose, iBV UMR CNRS 7277/Inserm 1091/Universite Cote d'Azur
attributes.tag: INSDC center name
attributes.value: Institut de Biologie Valrose, iBV UMR CNRS 7277/Inserm 1091/Universite Cote d'Azur
attributes.tag: INSDC first public
attributes.value: 2023-06-16T00:26:33Z
attributes.tag: INSDC last update
attributes.value: 2023-06-16T00:26:33Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12938:NG-6612_R1_dnCad_lib21093_1481_2
attributes.tag: age
attributes.value: 6.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: common urchin
attributes.tag: developmental stage
attributes.value: blastula
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: phenotype
attributes.value: overexpression of dominant negative cadherin
attributes.tag: replicate
attributes.value: A
attributes.tag: sample name
attributes.value: E-MTAB-12938:NG-6612_R1_dnCad_lib21093_1481_2
attributes.tag: scientific_name
attributes.value: Paracentrotus lividus
attributes.tag: stimulus
attributes.value: dnCad