title: EMOBON_ROSKOGO_Wa_18
organism: marine plankton metagenome
description: EMOBON metagenome water sample from station ROSKOGO collected on 2021-08-02 size fraction 0.2-3um
attributes.tag: ENA first public
attributes.value: 2024-11-11
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00022
attributes.tag: amount or size of sample collected
attributes.value: 7.5
attributes.tag: broad-scale environmental context
attributes.value: marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023]
attributes.tag: collection date
attributes.value: 2021-08-02
attributes.tag: depth
attributes.value: 2
attributes.tag: dna concentration
attributes.value: 32.5
attributes.tag: environmental medium
attributes.value: seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
attributes.tag: geographic location (country and/or sea)
attributes.value: France
attributes.tag: geographic location (latitude)
attributes.value: 48.771667
attributes.tag: geographic location (locality)
attributes.value: French part of the English Channel
attributes.tag: geographic location (longitude)
attributes.value: -3.968333
attributes.tag: geographic location (ocean)
attributes.value: North Atlantic Ocean
attributes.tag: geographic location (region and locality)
attributes.value: English Channel
attributes.tag: library reads sequenced
attributes.value: 52880056
attributes.tag: local environmental context
attributes.value: marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
attributes.tag: mock_pr
attributes.value: DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
attributes.tag: obs_id
attributes.value: ROSKOGO
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: relevant standard operating procedures
attributes.value: Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
attributes.tag: sample collection device
attributes.value: Hand pump
attributes.tag: sample material processing
attributes.value: WaSOP 1
attributes.tag: sample name
attributes.value: EMOBON00022
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: marine plankton metagenome
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch1
attributes.tag: sequence_batch_rundate
attributes.value: Batch1_220712
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: size-fraction lower threshold
attributes.value: 0.2
attributes.tag: size-fraction upper threshold
attributes.value: 3
attributes.tag: source material identifiers
attributes.value: EMOBON_ROSKOGO_Wa_210802_0.2um_2
attributes.tag: status
attributes.value: sample
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage
attributes.value: high
attributes.tag: tidal stage information
attributes.value: flood_tide
attributes.tag: total depth of water column
attributes.value: 60