title: EMOBON_NRMCB_So_10
organism: metagenome
description: EMOBON metagenome sediment sample from station NRMCB collected on 2021-08-31 community collected micro
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: Submitter Id
attributes.value: EMOBON00099
attributes.tag: broad-scale environmental context
attributes.value: Control
attributes.tag: collection date
attributes.value: 2021-08-31
attributes.tag: depth
attributes.value: 0
attributes.tag: elevation
attributes.value: 0
attributes.tag: environmental medium
attributes.value: swab
attributes.tag: geographic location (country and/or sea)
attributes.value: missing: control sample
attributes.tag: geographic location (latitude)
attributes.value: missing: control sample
attributes.tag: geographic location (locality)
attributes.value: missing: control sample
attributes.tag: geographic location (longitude)
attributes.value: missing: control sample
attributes.tag: geographic location (ocean)
attributes.value: missing: control sample
attributes.tag: geographic location (region and locality)
attributes.value: missing: control sample
attributes.tag: library reads sequenced
attributes.value: 43917269
attributes.tag: local environmental context
attributes.value: Negative
attributes.tag: mock_pr
attributes.value: DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
attributes.tag: obs_id
attributes.value: NRMCB
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:2;Nocycles:18
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: relevant standard operating procedures
attributes.value: Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
attributes.tag: sample name
attributes.value: EMOBON00099
attributes.tag: sample storage location
attributes.value: freezer #1 BEOM; upper compartment; rack #8 d III (see scheme in technical support EMI)
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: metagenome
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch1
attributes.tag: sequence_batch_rundate
attributes.value: Batch1_220712
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: source material identifiers
attributes.value: EMOBON_NRMCB_So_210831_micro_blank
attributes.tag: status
attributes.value: blank
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage information
attributes.value: no_tide
attributes.tag: total depth of water column
attributes.value: 75