title: VH2_7
organism: Bos taurus
description: Protocols: Peripheral blood mononuclear cells (PBMC) were prepared from heparinised blood using Histopaque and frozen in foetal calf serum supplemented with 10% DMSO. Cells were defrosted into warm RPMI GlutaMAX HEPES media supplemented with 10% foetal calf serum, 1 mM sodium pyruvate, 50 µM 2-mercaptoethanol, 100 IU/mL penicillin and 100 µg/mL streptomycin (RPMI/10). Nucleic acid was extracted from one million cells using a MagMAX mirvRNA Total RNA extraction kit on a MagMAX™ Express-96 Deep Well Magnetic Particle Processor. 3'UTR sequencing
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-15
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-15
attributes.tag: External Id
attributes.value: SAMEA113483584
attributes.tag: INSDC center alias
attributes.value: Researcher
attributes.tag: INSDC center name
attributes.value: Researcher
attributes.tag: INSDC first public
attributes.value: 2023-06-15T00:18:12Z
attributes.tag: INSDC last update
attributes.value: 2023-06-15T00:18:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12965:VH2_7
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: mononuclear cell
attributes.tag: common name
attributes.value: cattle
attributes.tag: growth condition
attributes.value: challenge with viral infection
attributes.tag: individual
attributes.value: VH2
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-12965:VH2_7
attributes.tag: scientific_name
attributes.value: Bos taurus