title: Amplicon Sequencing (ITS2) of pooled oat root sample, August block 2 composted wood fiber plot
organism: root metagenome
description: Oat root sample, part of a study of root-associated fungal communities in QVIDJA organic soil amendment experiment
attributes.tag: environment (material)
attributes.value: clayey field soil
attributes.tag: collection date
attributes.value: 2018-08-20
attributes.tag: target gene
attributes.value: rRNA
attributes.tag: geographic location (longitude)
attributes.value: 22.39328
attributes.tag: host subject id
attributes.value: 235,236,237
attributes.tag: host common name
attributes.value: oats
attributes.tag: sample material processing
attributes.value: freezing, storing in -20 degrees, root washing, freeze-drying
attributes.tag: host-associated environmental package
attributes.value: host-associated
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-01-05T00:25:34Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-01-05T00:25:34Z
attributes.tag: environment (biome)
attributes.value: hemiboreal zone
attributes.tag: environment (feature)
attributes.value: agricultural field
attributes.tag: experimental factor
attributes.value: composted wood fiber (CWF) August
attributes.tag: geographic location (country and/or sea)
attributes.value: Finland
attributes.tag: sample storage temperature
attributes.value: -20
attributes.tag: organism
attributes.value: root metagenome
attributes.tag: sample collection device or method
attributes.value: adherent soil and roots were collected with clean shovel from 15 cm x 15 cm area down to 10 cm depth
attributes.tag: host taxid
attributes.value: 4498
attributes.tag: geographic location (latitude)
attributes.value: 60.29547
attributes.tag: scientific_name
attributes.value: root metagenome
attributes.tag: common name
attributes.value: ITS2 amplicons, oat root sample
attributes.tag: target subfragment
attributes.value: ITS2
attributes.tag: host substrate
attributes.value: clayey field soil
attributes.tag: investigation type
attributes.value: mimarks-specimen
attributes.tag: sample storage duration
attributes.value: 3
attributes.tag: pcr primers
attributes.value: FWD: GTGARTCATCGAATCTTTG; REV: TCCTCCGCTTATTGATATGC
attributes.tag: host body site
attributes.value: root
attributes.tag: project name
attributes.value: QVIDJA soil organic amendment experiment
attributes.tag: sequencing method
attributes.value: Illumina Miseq
attributes.tag: host age
attributes.value: 97
attributes.tag: host dry mass
attributes.value: 48,054
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-01-05
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-01-05