title: Organoid PD replicate 1
organism: Homo sapiens
description: Protocols: Cells from 2D culture were scraped in RLT buffer (QIAGEN RNeasy kit). Organoids were dissolved in RLT buffer and passed 10-times through a 21G needle. Note: the \"replicate 1\" of 2D control and PD have 3 data files, corresponding to 3 differentiation runs carried out at the same time, e.g. derived from the same cell populations. QIAGEN RNeasy kits (Mini for 2D culture and Micro for organoids) according to manufacturer's handbook. Library was prepared with NEBNext Ultra II RNA Library Prep Kit (New England BioLabs). For the 2D cultures, 500 ng of RNA were used and 5 cycles of PCR amplification were performed, whereas for the organoids, 120 ng RNA were used and 9 cycles of PCR amplification were performed. AMPure XP (Beckman Coulter) was used for purification. Fragment Analyzer (Agilent) was used for quality assessment.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-30T00:24:15Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-30T00:24:15Z
attributes.tag: External Id
attributes.value: SAMEA112484116
attributes.tag: INSDC center alias
attributes.value: Department of Biomedical Sciences, University of Copenhagen, Denmark
attributes.tag: INSDC center name
attributes.value: Department of Biomedical Sciences, University of Copenhagen, Denmark
attributes.tag: INSDC first public
attributes.value: 2023-06-30T00:24:15Z
attributes.tag: INSDC last update
attributes.value: 2023-06-30T00:24:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12573:Organoid PD replicate 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: H9
attributes.tag: cell_type
attributes.value: cardiocyte
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: organoid
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: inner cell mass
attributes.tag: progenitor cell type
attributes.value: embryonic stem cell
attributes.tag: sample name
attributes.value: E-MTAB-12573:Organoid PD replicate 1
attributes.tag: scientific_name
attributes.value: Homo sapiens