title: EMOBON_BPNS_So_18
organism: marine sediment metagenome
description: EMOBON metagenome sediment sample from station BPNS collected on 2021-10-28 community collected micro
attributes.tag: ENA first public
attributes.value: 2025-04-24
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00141
attributes.tag: broad-scale environmental context
attributes.value: marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
attributes.tag: collection date
attributes.value: 2021-10-28
attributes.tag: depth
attributes.value: 18.66
attributes.tag: dissolved oxygen
attributes.value: 8.14
attributes.tag: dissolved oxygen method
attributes.value: CTD
attributes.tag: dna concentration
attributes.value: 2.91
attributes.tag: elevation
attributes.value: -18.66
attributes.tag: environmental medium
attributes.value: sediment [ENVO:00002007]
attributes.tag: geographic location (country and/or sea)
attributes.value: Belgium
attributes.tag: geographic location (latitude)
attributes.value: 51.433331
attributes.tag: geographic location (locality)
attributes.value: Belgian part of the North Sea
attributes.tag: geographic location (longitude)
attributes.value: 2.808331
attributes.tag: geographic location (ocean)
attributes.value: North Atlantic Ocean
attributes.tag: geographic location (region and locality)
attributes.value: North Sea
attributes.tag: library reads sequenced
attributes.value: 88875433
attributes.tag: local environmental context
attributes.value: shallow_marine_sediment_[ENVO:03000034]
attributes.tag: mock_pr
attributes.value: DDC_AAAAOSDA_1_HCFCYDSX5.UDI137
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
attributes.tag: obs_id
attributes.value: BPNS
attributes.tag: pH
attributes.value: 7
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Kapa;NoPCRs:2;PCRvolume:30;Nocycles:12
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: ph method
attributes.value: Hanna Instruments 991001 ph meter
attributes.tag: pressure
attributes.value: 1.00014803849
attributes.tag: pressure method
attributes.value: CTD
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: relevant standard operating procedures
attributes.value: Prod_ILL_BqADN_LowCost_148_v5; Bq low cost
attributes.tag: salinity
attributes.value: 33.855
attributes.tag: salinity method
attributes.value: CTD
attributes.tag: sample collection device
attributes.value: Van Veen Grab
attributes.tag: sample material processing
attributes.value: SoSOP3
attributes.tag: sample name
attributes.value: EMOBON00141
attributes.tag: sample storage location
attributes.value: MSO LifeWatch Lab -80 Freezer
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: marine sediment metagenome
attributes.tag: sea surface temperature
attributes.value: 14.097
attributes.tag: sea surface temperature method
attributes.value: CTD
attributes.tag: sediment temperature
attributes.value: 14.099
attributes.tag: sediment temperature method
attributes.value: CTD
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch2
attributes.tag: sequence_batch_rundate
attributes.value: Batch2_230126
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: source material identifiers
attributes.value: EMOBON_BPNS_So_211028_micro_2
attributes.tag: status
attributes.value: sample
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage
attributes.value: low
attributes.tag: tidal stage information
attributes.value: ebb_tide
attributes.tag: total depth of water column
attributes.value: 26