title: Sample 1
organism: Homo sapiens
description: Protocols: Adult human adrenal samples for scRNA-seq were collected from two Saitama Medical University International Medical Center cases. Samples 1 and 2 were obtained from a 62-year-old male at radical nephrectomy for renal cell carcinoma and a 29-year-old male at adrenalectomy for pheochromocytoma. CD24/CD31/CD45 positive cells were removed using FACS and adrenocortical cells were enriched. The libraries of scRNA-seq were prepared from the cell suspensions using Chromium controller (10X Genomics) and Chromium Single Cell 3' Reagent Kits v3.1 according to the manufacturer's instructions. The libraries of scRNA-seq were prepared from the cell suspensions using Chromium controller (10X Genomics) and Chromium Single Cell 3' Reagent Kits v3.1 according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-07-05
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-07-05
attributes.tag: External Id
attributes.value: SAMEA110183771
attributes.tag: INSDC center alias
attributes.value: Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University
attributes.tag: INSDC center name
attributes.value: Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University
attributes.tag: INSDC first public
attributes.value: 2022-07-05T12:04:00Z
attributes.tag: INSDC last update
attributes.value: 2022-07-05T12:04:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11837:Sample 1
attributes.tag: age
attributes.value: 62
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: cortical cell of adrenal gland
attributes.tag: clinical information
attributes.value: radical nephrectomy
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: renal cell carcinoma
attributes.tag: immunophenotype
attributes.value: CD24-; CD31-; CD45-
attributes.tag: individual
attributes.value: SIMC-Uro #12142
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: adrenal cortex
attributes.tag: sample name
attributes.value: E-MTAB-11837:Sample 1
attributes.tag: sex
attributes.value: male