organism: freshwater metagenome
attributes.tag: collection_date
attributes.value: 2021/2023
attributes.tag: depth
attributes.value: 0
attributes.tag: env_broad_scale
attributes.value: watershed [ENVO_00000292]
attributes.tag: env_local_scale
attributes.value: stream [ENVO_00000023]
attributes.tag: env_medium
attributes.value: water surface [ENVO_01001191]
attributes.tag: geo_loc_name
attributes.value: USA:Iowa,Ames
attributes.tag: lat_lon
attributes.value: 42.0319 N 93.6545 W
attributes.tag: library_id
attributes.value: CC20223IZ2
attributes.tag: title
attributes.value: 16S rRNA metagenome from an intermittent freshwater creek
attributes.tag: library_strategy
attributes.value: AMPLICON
attributes.tag: library_source
attributes.value: METAGENOMIC
attributes.tag: library_selection
attributes.value: PCR
attributes.tag: library_layout
attributes.value: paired
attributes.tag: platform
attributes.value: ILLUMINA
attributes.tag: instrument_model
attributes.value: Illumina MiSeq
attributes.tag: design_description
attributes.value: 500 ml of water filtered onto 3 _m filters. DNA was extracted with the DNeasy Powerbiofilm Isolation kit. DNA was quantified with a Qubit 2.0 Fluorometer. Illumina MiSeq (2x250 bp) was used to sequence the V4 region of the 16S rRNA gene with the primer pair 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 805R (5-GACTACVSGGGTATCTAAT-3) using a dual index approach.
attributes.tag: filetype
attributes.value: fastq
attributes.tag: filename
attributes.value: CC20223IZ2_S15_L001_R1_001.fastq
attributes.tag: filename2
attributes.value: CC20223IZ2_S15_L001_R2_001.fastq
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.water