title: A309_dilue
organism: Mus musculus
description: Protocols: Sciatic nerves from P4 animals of different genotypes were dissected in PBS Total RNA from was extracted using RNeasy Mini kit (Qiagen) according to the manufacturerâ€™s instructions. After DNAse treatment, concentration and quality of RNA was determined using the NanoDrop (Thermofisher) and Xpose (Trinean) apparatus. RNAseq libraries were prepared starting from 60 ng of total RNA using the Universal Plus mRNA-Seq (Nugen) as recommended by the manufacturer. The oriented cDNA produced from the poly-A+ fraction were PCR amplified (15 cycles).
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-11-03T00:39:07Z
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-11-03T00:39:07Z
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: common name
attributes.value: house mouse
attributes.tag: organism part
attributes.value: sciatic nerve
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-11-03
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-11-03