title: Sample 13
organism: Mus musculus
description: Protocols: Euthanized animals were perfused with 20 ml of PBS before hearts were harvested. Mice were treated i.p. twice per week with 200 µg of 14-D10-2 anti-Gremlin monoclonal antibodies or IgG2b isotype control. For enzymatic dissociation hearts were minced into small pieces and were placed into a six-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (Lonza), 1 mg/ml collagenase D (Sigma), and 25 μg/ml DNase I (Applichem) and incubated at 37°C under continuous stirring. The remaining tissue pieces were mechanically disrupted. For analysis of total cardiac cells, single cell suspensions of freshly isolated murine hearts were sorted for total live cells using a BDFACSMelody. Fibroblast samples were sorted for live CD45-CD31-PDPN+ cells using a BDFACSMelody. Sorted single cells suspensions were emulsified for library generation using the droplet-based 10x Chromium (10x Genomics) system. cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3' Reagent Kit (v3 Chemistry).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-01-17T16:00:07Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-01-17T16:00:07Z
attributes.tag: External Id
attributes.value: SAMEA112495249
attributes.tag: INSDC center name
attributes.value: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
attributes.tag: INSDC first public
attributes.value: 2024-01-17T16:00:07Z
attributes.tag: INSDC last update
attributes.value: 2024-01-17T16:00:07Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12589:Sample 13
attributes.tag: age
attributes.value: 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: myocarditis
attributes.tag: genotype
attributes.value: MYH6-specific TCR transgene
attributes.tag: individual
attributes.value: Sample 13
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: heart
attributes.tag: sample name
attributes.value: E-MTAB-12589:Sample 13
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: BALB/c