title: A297_dilue
organism: Mus musculus
description: Protocols: Sciatic nerves from P4 animals of different genotypes were dissected in PBS Total RNA from was extracted using RNeasy Mini kit (Qiagen) according to the manufacturer's instructions. After DNAse treatment, concentration and quality of RNA was determined using the NanoDrop (Thermofisher) and Xpose (Trinean) apparatus. RNAseq libraries were prepared starting from 60 ng of total RNA using the Universal Plus mRNA-Seq (Nugen) as recommended by the manufacturer. The oriented cDNA produced from the poly-A+ fraction were PCR amplified (15 cycles).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-11-03T00:39:07Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-11-03T00:39:07Z
attributes.tag: External Id
attributes.value: SAMEA11305862
attributes.tag: INSDC center name
attributes.value: INSERM
attributes.tag: INSDC first public
attributes.value: 2023-11-03T00:39:07Z
attributes.tag: INSDC last update
attributes.value: 2023-11-03T00:39:07Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11197:A297_dilue
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: sciatic nerve
attributes.tag: sample name
attributes.value: E-MTAB-11197:A297_dilue
attributes.tag: scientific_name
attributes.value: Mus musculus