title: DFA35 - Sample 1
organism: Burkholderia cenocepacia
description: Protocols: Overnight liquid cultures of each isolate, B. cenocepacia wild type and each mutant, that were grown at 37°C while shaking at 200 rpm in SCFM, were transferred to fresh media until the mid-exponential phase was reached. The cultures were subsequently diluted to a standardized culture to OD600nm 0.4 in 250 ml glass flasks. These cultures were further incubated for 18 hours before centrifugation at 4°C and the obtained cell pellets stored at −80 °C for a maximum of 1 week. Cells were grown at 37°C while shaking at 200 rpm in synthetic CF medium (SCFM). RNA extraction was outsourced to GENEWIZ Europe (Leipzig, Germany). Library prep was outsourced to GENEWIZ Europe (Leipzig, Germany).
attributes.tag: ENA first public
attributes.value: 2025-03-03
attributes.tag: External Id
attributes.value: SAMEA115084279
attributes.tag: INSDC center name
attributes.value: Protein Research Unit - PRU Laboratory of Microbiology - LM Department Biochemistry and Microbiology Ghent University
attributes.tag: INSDC last update
attributes.value: 2024-01-05T17:13:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13688:DFA35 - Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: genetically modified strain
attributes.tag: collection date
attributes.value: not collected
attributes.tag: genetic modification
attributes.value: with the deletion of atsR (bcam0379) gene
attributes.tag: genotype
attributes.value: deficient derivative of the worldwide ET-12 epidemic clones
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth condition
attributes.value: in synthetic CF medium (SCFM), the most in-vivo-like culturing medium, at 37°C and 200 rpm.
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-13688:DFA35 - Sample 1
attributes.tag: scientific_name
attributes.value: Burkholderia cenocepacia
attributes.tag: strain
attributes.value: DFA35