title: EMOBON_AAOT_Wa_67
organism: marine plankton metagenome
description: EMOBON metagenome water sample from station AAOT collected on 2021-12-14 size fraction 0.2-3um
attributes.tag: ENA first public
attributes.value: 2025-04-24
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00169
attributes.tag: ammonium
attributes.value: 0.14
attributes.tag: amount or size of sample collected
attributes.value: 5
attributes.tag: broad-scale environmental context
attributes.value: marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023];mediterranean sea biome[ENVO_01000047]
attributes.tag: chlorophyll
attributes.value: 0.32
attributes.tag: chlorophyll method
attributes.value: filtration on 0.2 um FG filters. extraction in acetone overnight. fluorimetric measurement
attributes.tag: collection date
attributes.value: 2021-12-14
attributes.tag: depth
attributes.value: 1
attributes.tag: dna concentration
attributes.value: 4.22
attributes.tag: environmental medium
attributes.value: seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
attributes.tag: geographic location (country and/or sea)
attributes.value: Italy
attributes.tag: geographic location (latitude)
attributes.value: 45.314167
attributes.tag: geographic location (locality)
attributes.value: Gulf of Venice
attributes.tag: geographic location (longitude)
attributes.value: 12.508333
attributes.tag: geographic location (ocean)
attributes.value: Mediterranean Sea - Eastern Basin
attributes.tag: geographic location (region and locality)
attributes.value: Adriatic Sea
attributes.tag: library reads sequenced
attributes.value: 60334631
attributes.tag: local environmental context
attributes.value: marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
attributes.tag: mock_eu
attributes.value: DDC_AAABOSDA_2_HVWGWDSX5.UDI143;DDC_AAABOSDA_2_HVWGWDSX5.UDI217
attributes.tag: mock_pr
attributes.value: DDC_AAACOSDA_1_HVWGWDSX5.UDI164;DDC_AAACOSDA_1_HVWGWDSX5.UDI218
attributes.tag: nitrate
attributes.value: 4.99
attributes.tag: nitrite
attributes.value: 1.51
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
attributes.tag: obs_id
attributes.value: AAOT
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: phosphate
attributes.value: 0.11
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: salinity
attributes.value: 36.8
attributes.tag: salinity method
attributes.value: Seabird SBE 911plus CTD system
attributes.tag: sample collection device
attributes.value: Niskin
attributes.tag: sample material processing
attributes.value: WaSOP 1
attributes.tag: sample name
attributes.value: EMOBON00169
attributes.tag: sample storage location
attributes.value: DeepFreezer No2, second shelf, drawer 2
attributes.tag: sample storage temperature
attributes.value: -78
attributes.tag: scientific_name
attributes.value: marine plankton metagenome
attributes.tag: sea subsurface temperature
attributes.value: 12.39
attributes.tag: sea subsurface temperature method
attributes.value: Seabird SBE 911plus CTD system
attributes.tag: sea surface temperature
attributes.value: 10.78
attributes.tag: sea surface temperature method
attributes.value: Seabird SBE 911plus CTD system
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch2
attributes.tag: sequence_batch_rundate
attributes.value: Batch2_230428
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: silicate
attributes.value: 7.42
attributes.tag: size-fraction lower threshold
attributes.value: 0.2
attributes.tag: size-fraction upper threshold
attributes.value: 3
attributes.tag: source material identifiers
attributes.value: EMOBON_AAOT_Wa_211214_0.2um_2
attributes.tag: status
attributes.value: sample
attributes.tag: subsurface salinity
attributes.value: 37.78
attributes.tag: subsurface salinity method
attributes.value: Seabird SBE 911plus CTD system
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage
attributes.value: high
attributes.tag: tidal stage information
attributes.value: flood_tide
attributes.tag: total depth of water column
attributes.value: 17
attributes.tag: turbidity
attributes.value: 2.2