title: HK211
organism: Homo sapiens
description: Protocols: Patient brain tumor samples were collected during surgery and glioma stem cells were purified from the samples. Glioma cells were made to grow into gliomaspheres in a growth factor cocktail containing media, prior to centrifugation and nucleic acid extraction. Total RNA was extracted and purified from cellular samples using TRIzol Reagent (Ambion, Cat# 15596018), while mRNA was purified using PolyATract mRNA Isolation Systems Kit (Promega, Cat#Z5300) from total RNA samples. RNA transcripts were fragmented into ~100 nucleotide segments and isolated for m6A enrichment using m6A Dynabeads (Synaptic Systems, Cat# 202003) for each sample. This allowed for input RNA and m6A enriched RNA to be analyzed following MeRIP-Seq using RADAR. Stranded mRNA Library Preparation Kits (Illumina, Cat# 20020594) were used for sequencing library preparations, with unique adapter indices ligated to each sample to allow for multiplexing (TruSeq RNA Single Indices Set A, Illumina, Cat# 20020492).
attributes.tag: ENA first public
attributes.value: 2024-02-17
attributes.tag: INSDC center name
attributes.value: UCLA
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13765:HK211
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: gliomasphere
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: glioma
attributes.tag: genotype
attributes.value: IDH-Mut
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: brain
attributes.tag: progenitor cell type
attributes.value: glioma stem cell
attributes.tag: sample name
attributes.value: E-MTAB-13765:HK211
attributes.tag: sampling site
attributes.value: tumor
attributes.tag: scientific_name
attributes.value: Homo sapiens