title: ELE10_2
organism: Homo sapiens
description: Protocols: Cells (iAstrocytes) were detached with Accutase, counted and 500,000 cells were lysed with TRI-Reagent (Sigma). iAstrocytes were grown on hESc-qualified Matrigel (Corning)-coated plates, in Astrocyte Medium (ScienCell). Seeding density was 15,000 cells/sq. cm. RNA isolation was isolated using Direct-Zol RNA Isolation kit (ZYMO), according to the manufacturer's instructions. 1 ug of RNA (RIN > 8) was used for library constructions. Libraries were prepared using KAPA HyperPrep Kit, according to manufacturer's instructions. UDI/UMI (itDNA) indexes were used. Library quality was assessed with 4200 TapeStation System, High Sensitivity D1000 ScreenTape (Agilent).
attributes.tag: ENA first public
attributes.value: 2025-03-20
attributes.tag: External Id
attributes.value: SAMEA114262065
attributes.tag: INSDC center name
attributes.value: Nencki Institute of Experimental Biology
attributes.tag: INSDC last update
attributes.value: 2023-08-14T10:26:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13252:ELE10_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: ELE10
attributes.tag: cell_type
attributes.value: astrocyte
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: juvenile stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: skin
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: sample name
attributes.value: E-MTAB-13252:ELE10_2
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female