title: Sample 17
organism: Mus musculus
description: Protocols: Primary cortical neuron culture was established from C57BL/6 neonatal mice aged 1-3 days by collecting brain tissue from the cerebral cortex after decapitation, and divided into control group, OGD/R group, and breviscapine administration group. Total RNA was isolated and purified by TRIzol reagent following the instruction manual. These compounds doublets of DNA and RNA were converted into DNA doublets, dUTP Solution was incorporated into the doublets at the same time, and the ends of the double-stranded DNA were complemented to flat ends. An additional base was added to each end to enable ligation with a linker with a T base at the end, and its fragment size was screened and purified using magnetic beads. The second strand was digested with UDG enzyme, and then PCR was performed to form a library with a fragment size of 300bp Â± 50bp.
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: environmental stress
attributes.value: oxygen-glucose deprivation/reoxygenation
attributes.tag: disease
attributes.value: stroke
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: study group
attributes.value: OGD/R
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: common name
attributes.value: house mouse
attributes.tag: organism part
attributes.value: brain
attributes.tag: developmental stage
attributes.value: neonate
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: growth condition
attributes.value: primary cortical neuron culture
attributes.tag: cell_type
attributes.value: neuron
attributes.tag: age
attributes.value: 1 to 3
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-05-25