title: SCR_2
organism: Homo sapiens
description: Protocols: Cells were collected after 24 hours from lncMAAT silencing. Cells were cultured in RPMI-1640 medium supplemented with 10% FBS and 20U/ml IL2. TLBR-2 cells were transfected with 50nM Antisense LNA GapmeRs concentration. Antisense LNA GapmeR transfections was performed using the Cell Line Nucleofector Kit SF and Amaxa 4D Nucleofector (program DS-130). 24 hours after transfection, cells were harvested for RNA extraction. Antisense LNA GapmeR Negative Control (Cat. No. / ID: 33951, Qiagen) was used as negative control. Total RNA from cells was extracted by TRIzol (Thermo Fisher Scientific) according to the manufacture's instructions. Libraries were obtained starting from 100ng of total RNA following the Illumina Stranded Total RNA preparation Ligation with Ribo-Zero Plus protocol.
attributes.tag: ENA first public
attributes.value: 2024-12-13
attributes.tag: External Id
attributes.value: SAMEA112232476
attributes.tag: INSDC center name
attributes.value: Laboratory of Traslational Research. Azienda USL-IRCCS di Reggio Emilia
attributes.tag: INSDC last update
attributes.value: 2022-12-13T16:26:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12462:SCR_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: TLBR2
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: anaplastic large cell lymphoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: breast
attributes.tag: sample name
attributes.value: E-MTAB-12462:SCR_2
attributes.tag: scientific_name
attributes.value: Homo sapiens