title: Sample 19
organism: Mus musculus
description: Protocols: Primary cortical neuron culture was established from C57BL/6 neonatal mice aged 1-3 days by collecting brain tissue from the cerebral cortex after decapitation, and divided into control group, OGD/R group, and breviscapine administration group. Total RNA was isolated and purified by TRIzol reagent following the instruction manual. These compounds doublets of DNA and RNA were converted into DNA doublets, dUTP Solution was incorporated into the doublets at the same time, and the ends of the double-stranded DNA were complemented to flat ends. An additional base was added to each end to enable ligation with a linker with a T base at the end, and its fragment size was screened and purified using magnetic beads. The second strand was digested with UDG enzyme, and then PCR was performed to form a library with a fragment size of 300bp ± 50bp.
attributes.tag: ENA first public
attributes.value: 2024-05-17
attributes.tag: External Id
attributes.value: SAMEA113550145
attributes.tag: INSDC center name
attributes.value: The First Affiliated Hospital of Kunming Medical University
attributes.tag: INSDC last update
attributes.value: 2023-05-25T10:57:28Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13017:Sample 19
attributes.tag: age
attributes.value: 1 to 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: neuron
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: neonate
attributes.tag: disease
attributes.value: stroke
attributes.tag: environmental stress
attributes.value: oxygen-glucose deprivation/reoxygenation
attributes.tag: growth condition
attributes.value: primary cortical neuron culture
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-13017:Sample 19
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: study group
attributes.value: OGD/R