title: EMOBON_RFormosa_Wa_21
organism: marine plankton metagenome
description: EMOBON metagenome water sample from station RFormosa collected on 2021-12-20 size fraction 3-200um
attributes.tag: ENA first public
attributes.value: 2025-04-24
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00218
attributes.tag: amount or size of sample collected
attributes.value: 5
attributes.tag: broad-scale environmental context
attributes.value: marine biome [ENVO:00000447]
attributes.tag: chlorophyll
attributes.value: 3.98132
attributes.tag: chlorophyll method
attributes.value: Fluorometer 10-AU
attributes.tag: collection date
attributes.value: 2021-12-20
attributes.tag: depth
attributes.value: 0.2
attributes.tag: dissolved oxygen
attributes.value: 256.25
attributes.tag: dissolved oxygen method
attributes.value: YSI ProSolo Digital
attributes.tag: dna concentration
attributes.value: 5.22
attributes.tag: environmental medium
attributes.value: lagoon [ENVO_00000038];marine water body [ENVO:00001999]; surface water [ENVO:00002042]
attributes.tag: geographic location (country and/or sea)
attributes.value: Portugal
attributes.tag: geographic location (latitude)
attributes.value: 37.005639
attributes.tag: geographic location (locality)
attributes.value: Ria Formosa
attributes.tag: geographic location (longitude)
attributes.value: -7.96925
attributes.tag: geographic location (ocean)
attributes.value: Atlantic Ocean
attributes.tag: geographic location (region and locality)
attributes.value: North Atlantic Ocean
attributes.tag: library reads sequenced
attributes.value: 58304897
attributes.tag: local environmental context
attributes.value: lagoon [ENVO_00000038];marine water body [ENVO:00001999]
attributes.tag: mock_eu
attributes.value: DDC_AAABOSDA_2_HVWGWDSX5.UDI143;DDC_AAABOSDA_2_HVWGWDSX5.UDI217
attributes.tag: mock_pr
attributes.value: DDC_AAACOSDA_1_HVWGWDSX5.UDI164;DDC_AAACOSDA_1_HVWGWDSX5.UDI218
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
attributes.tag: obs_id
attributes.value: RFormosa
attributes.tag: pH
attributes.value: 8.258
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: ph method
attributes.value: Thermo scientific orion star A221
attributes.tag: pressure
attributes.value: 1.005181347
attributes.tag: pressure method
attributes.value: YSI ProSolo Digital
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: salinity
attributes.value: 36.3
attributes.tag: salinity method
attributes.value: YSI ProSolo Digital
attributes.tag: sample collection device
attributes.value: bucket
attributes.tag: sample material processing
attributes.value: WaSOP 1
attributes.tag: sample name
attributes.value: EMOBON00218
attributes.tag: sample storage location
attributes.value: Ed7, room 1.10, freezer #300387-118
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: marine plankton metagenome
attributes.tag: sea subsurface temperature
attributes.value: 15
attributes.tag: sea subsurface temperature method
attributes.value: Thermo scientific orion star A221
attributes.tag: sea surface temperature
attributes.value: 15
attributes.tag: sea surface temperature method
attributes.value: Thermo scientific orion star A221
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch2
attributes.tag: sequence_batch_rundate
attributes.value: Batch2_230428
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: size-fraction lower threshold
attributes.value: 3
attributes.tag: size-fraction upper threshold
attributes.value: 200
attributes.tag: source material identifiers
attributes.value: EMOBON_RFormosa_Wa_211220_3um_1
attributes.tag: status
attributes.value: sample
attributes.tag: subsurface salinity
attributes.value: 36.3
attributes.tag: subsurface salinity method
attributes.value: YSI ProSolo Digital
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage
attributes.value: low
attributes.tag: tidal stage information
attributes.value: low_tide
attributes.tag: total depth of water column
attributes.value: 1