title: BACMIX_minion_v1_9_20230623_zymo_magbead_NZ1
organism: gut metagenome
description: ZymoBIOMICS Microbial Community Standard
attributes.tag: chimera check software
attributes.value: minitax
attributes.tag: broad-scale environmental context
attributes.value: not applicable
attributes.tag: host scientific name
attributes.value: not applicable
attributes.tag: sequence quality check
attributes.value: LongQC
attributes.tag: collection date
attributes.value: not collected
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: host family relationship
attributes.value: not applicable
attributes.tag: geographic location (longitude)
attributes.value: not applicable
attributes.tag: host subject id
attributes.value: not applicable
attributes.tag: host subspecific genetic lineage
attributes.value: not applicable
attributes.tag: host common name
attributes.value: not applicable
attributes.tag: host sex
attributes.value: not applicable
attributes.tag: temperature
attributes.value: 20
attributes.tag: nucleic acid extraction
attributes.value: ZymoBIOMICS 96 MagBead DNA Kit, Zymo
attributes.tag: nucleic acid amplification
attributes.value: LongAmp Taq 2X Master Mix
attributes.tag: geographic location (country and/or sea)
attributes.value: not applicable
attributes.tag: library reads sequenced
attributes.value: not provided
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: adapters
attributes.value: 16SFw 5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'; 16S Rev 5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'
attributes.tag: organism
attributes.value: gut metagenome
attributes.tag: library construction method
attributes.value: 16S Barcoding Kit (SQK-RAB204)
attributes.tag: library size
attributes.value: not provided
attributes.tag: host taxid
attributes.value: not applicable
attributes.tag: environmental medium
attributes.value: DNA/RNA Shield (R1100-50)
attributes.tag: geographic location (latitude)
attributes.value: not applicable
attributes.tag: scientific_name
attributes.value: gut metagenome
attributes.tag: target subfragment
attributes.value: V1-V9
attributes.tag: host diet
attributes.value: not applicable
attributes.tag: sample storage duration
attributes.value: 96h
attributes.tag: pcr primers
attributes.value: 27F 5'-AGAGTTTGATCCTGGCTCAG-3'; 1492R 5'-GGTTACCTTGTTACGACTT-3'
attributes.tag: host body product
attributes.value: not applicable
attributes.tag: pcr conditions
attributes.value: Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
attributes.tag: local environmental context
attributes.value: not applicable
attributes.tag: organism count
attributes.value: 10.5x10^9
attributes.tag: project name
attributes.value: Ultra-deep, hybrid metagenomic sequencing of canine gut microbiome
attributes.tag: sequencing method
attributes.value: Oxford Nanopore, SQK-RAB204
attributes.tag: host age
attributes.value: not applicable
attributes.tag: library screening strategy
attributes.value: ligation based
attributes.tag: multiplex identifiers
attributes.value: AACTAGGCACAGCGAGTCTTGGTT