title: RNA extracted from Saccharina latissima strain F2.27.2-49.
organism: Saccharina latissima
attributes.tag: Culture conditions
attributes.value: F2 sporophytes derived from a bulk gametophyte cross between two strains of Saccharina latissima collected at Perharidy (near Roscoff, north Finistere, Brittany, France, January 2016, strain SLPER139) and Saint Guenole (south Finistere, Brittany, France, October 2015, strain SLSTG055) were cultivated on an open water rope system in Morlaix Bay in 2017.The F1 hybrid was strain F1.27.2. Under laboratory conditions, three small tissue disks cut from the blade of an F2 sporophyte individual were acclimatised for 4 days at 13C and then heat stressed at 21C for 7 days followed by an incubation at 13C for 16 days to allow recovery before harvesting (recovery sample). Fv/Fm measurements were carried out after heat stress and after the recovery period.
attributes.tag: DNA or RNA extracted
attributes.value: RNA
attributes.tag: DNA or RNA quality (ratio 260/280)
attributes.value: 3
attributes.tag: ENA first public
attributes.value: 2024-05-15
attributes.tag: Extraction date
attributes.value: 09/11/2023
attributes.tag: Extraction method
attributes.value: Macherey-Nagel RNAplus plus 1 wash RW2
attributes.tag: Harvest date
attributes.value: 19/10/2017 + 17/11/2022
attributes.tag: INSDC center name
attributes.value: Genoscope CEA
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 49-21-1
attributes.tag: additional_details
attributes.value: na
attributes.tag: collection date
attributes.value: missing: data agreement established pre-2023
attributes.tag: generation
attributes.value: sporophyte
attributes.tag: geographic location (country and/or sea)
attributes.value: France
attributes.tag: geographic location (latitude)
attributes.value: missing: data agreement established pre-2023
attributes.tag: geographic location (longitude)
attributes.value: missing: data agreement established pre-2023
attributes.tag: identification by
attributes.value: Zofia Nehr
attributes.tag: identification method
attributes.value: Morphology
attributes.tag: ploidy
attributes.value: diploid
attributes.tag: ratio 260/230
attributes.value: 2,18
attributes.tag: sample alias
attributes.value: 49-21-1
attributes.tag: sample description
attributes.value: RNA extracted from Saccharina latissima strain F2.27.2-49 for analysis of differential gene expression..
attributes.tag: sample name
attributes.value: 49-21-1
attributes.tag: sampling ecosystem
attributes.value: coastal
attributes.tag: sampling sea
attributes.value: Atlantic Ocean
attributes.tag: sampling site
attributes.value: Genetic cross of Perharidy and Saint Guenole strains, France
attributes.tag: sampling water type
attributes.value: marine
attributes.tag: scientific_name
attributes.value: Saccharina latissima
attributes.tag: sequencing centre sample code
attributes.value: CIT_FC
attributes.tag: sex
attributes.value: na
attributes.tag: sex determination method
attributes.value: na
attributes.tag: strain
attributes.value: F2.27.2-49
attributes.tag: tissue type
attributes.value: blade disks