title: Sample 16
organism: Homo sapiens
description: Protocols: Two pieces of tissue were obtained from patients that went through endomyocardial biopsy as part of the clinical routine practice of diagnostic or follow up procedure. For enzymatic dissociation hearts were minced into small pieces and were placed into a six-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (Lonza), 1 mg/ml collagenase D (Sigma), and 25 μg/ml DNase I (Applichem) and incubated at 37°C under continuous stirring. The remaining tissue pieces were mechanically disrupted. Nuclei were isolated from frozen patient derived EMBs and sorted to enrich intact nuclei using a BDFACSMelody. Sorted single nuclei suspensions were emulsified for library generation using the droplet-based 10x Chromium (10x Genomics) system. cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3' Reagent Kit (v3 Chemistry).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-01-17T16:00:27Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-01-17T16:00:27Z
attributes.tag: External Id
attributes.value: SAMEA112495209
attributes.tag: INSDC center name
attributes.value: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
attributes.tag: INSDC first public
attributes.value: 2024-01-17T16:00:27Z
attributes.tag: INSDC last update
attributes.value: 2024-01-17T16:00:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12584:Sample 16
attributes.tag: age
attributes.value: 62
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: cardiac transplant
attributes.tag: individual
attributes.value: ZH27
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: heart right ventricle
attributes.tag: sample name
attributes.value: E-MTAB-12584:Sample 16
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male