title: Transmission zone, strain none from peat:vermiculite
organism: soil metagenome
description: Soil from freshly x-ray sterilized soil
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-12-22T16:34:32Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-12-22T16:34:32Z
attributes.tag: External Id
attributes.value: SAMEA115057193
attributes.tag: INSDC center name
attributes.value: Swiss Federal Research Institute WSL
attributes.tag: INSDC first public
attributes.value: 2023-12-22T16:34:32Z
attributes.tag: INSDC last update
attributes.value: 2023-12-22T16:34:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: control2
attributes.tag: broad-scale environmental context
attributes.value: temperate woodland biome [ENVO_01000221]
attributes.tag: collection date
attributes.value: 2015-02
attributes.tag: depth
attributes.value: 0
attributes.tag: elevation
attributes.value: 467
attributes.tag: environmental medium
attributes.value: spruce forest soil [ENVO_00005784]
attributes.tag: geographic location (country and/or sea)
attributes.value: Switzerland
attributes.tag: geographic location (latitude)
attributes.value: 47.38195
attributes.tag: geographic location (longitude)
attributes.value: 8.47194
attributes.tag: local environmental context
attributes.value: forest ecosystem [ENVO_01001243]
attributes.tag: pcr conditions
attributes.value: PCR1 (primers with Nextera XT adapter overhang): duplicate 20 µl reactions with Kapa HiFi HotStart ReadyMix, 29 cycles
attributes.tag: pcr primers
attributes.value: forward: GATGAAGAACGYAGYRAA (ITS3_KYO2), reverse: TCCTCCGCTTATTGATATGC (ITS4), TCCTCGCCTTATTGATATGC (ITS4 variant)
attributes.tag: project name
attributes.value: Modes of local dispersal of PAC root endophytes
attributes.tag: sample name
attributes.value: control2
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: target gene
attributes.value: ITS
attributes.tag: target subfragment
attributes.value: ITS2