title: C_IR_T96_C
organism: freshwater metagenome
description: Treatment: Control; Light condition: IR; Replicate: C; Time: 96h
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-12-22T00:47:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-12-22T00:47:21Z
attributes.tag: External Id
attributes.value: SAMEA115020247
attributes.tag: INSDC center name
attributes.value: NMFRI
attributes.tag: INSDC first public
attributes.value: 2023-12-22T00:47:21Z
attributes.tag: INSDC last update
attributes.value: 2023-12-22T00:47:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: S222
attributes.tag: broad-scale environmental context
attributes.value: freshwater biome
attributes.tag: chimera check software
attributes.value: DADA2 version 1.18.0; function: removeBimeraDenovo; method=pooled
attributes.tag: collection date
attributes.value: 2018-10-05
attributes.tag: depth
attributes.value: 0.5
attributes.tag: environmental medium
attributes.value: coniferous forest
attributes.tag: geographic location (country and/or sea)
attributes.value: Czech Republic
attributes.tag: geographic location (latitude)
attributes.value: 48.924924
attributes.tag: geographic location (longitude)
attributes.value: 14.886811
attributes.tag: geographic location (region and locality)
attributes.value: South Bohemia, Lake Cep
attributes.tag: local environmental context
attributes.value: freshwater lake
attributes.tag: nucleic acid extraction
attributes.value: https://journals.asm.org/doi/10.1128/aem.71.11.6885-6899.2005
attributes.tag: organism count
attributes.value: total prokaryotes; 1792173.6 cells per ml; epifluorescent microscopy; Aerobic Anoxygenic Phototrophs; 55709.1 cells per ml; epifluorescent microscopy
attributes.tag: oxygenation status of sample
attributes.value: aerobic
attributes.tag: pcr conditions
attributes.value: intital denaturation:98degC_3min;denaturation:98degC_10s; annealing:52degC_30s;elongation:72degC_30s;number of cycles:30; final elongation:72degC_5min
attributes.tag: pcr primers
attributes.value: pufM_uniF: GGNAAYYTNTWYTAYAAY; pufM_uniR: YCCATNGTCCANCKCCARAACCNTTYCA
attributes.tag: project name
attributes.value: pufM study
attributes.tag: sample collection device
attributes.value: Ruttner Water Sampler (model 11.003KC)
attributes.tag: sample name
attributes.value: S222
attributes.tag: sample storage duration
attributes.value: less than 6 months
attributes.tag: sample storage location
attributes.value: Centre Algatech, freezere on the coridor
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 330
attributes.tag: scientific_name
attributes.value: freshwater metagenome
attributes.tag: sequencing method
attributes.value: Illumina MiSeq
attributes.tag: target gene
attributes.value: pufM
attributes.tag: total depth of water column
attributes.value: 10