title: 5386STDY7599667
organism: Mus musculus
description: Protocols: Three 19 months old and five 3 months old male mice of C57BL/6JRj strain were obtained from Janvier labs, France.They were used to dissect hindlimb muscles for the single-cell isolation according to the following protocol https://www.protocols.io/view/single-cell-isolation-from-human-skeletal-muscle-261geyydv479/v1. Briefly, muscle tissue was minced, digested in the solution of Collagenase II (Worthington-Biochem, LS004176) and Dispase (Gibco, 17105041) and, following that, the lysate was centrifuged in the gradient of Percoll to recover a fraction with single-cells. Cell or nuclei suspension were  loaded on the Chromium Controller (10X Genomics) with a targeted cell or nuclei recovery of 8000.  10X Genomics Single Cell 3â€™ v2 or v3 Reagent Kit was used to create GEM, perform cDNA synthesis and generate sequencing libraries according to standard instructions.
attributes.tag: individual
attributes.value: Mouse4
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: male
attributes.tag: cohort
attributes.value: old
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: common name
attributes.value: house mouse
attributes.tag: organism part
attributes.value: hindlimb muscle
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: injury
attributes.value: none
attributes.tag: cell_type
attributes.value: cell
attributes.tag: age
attributes.value: 19
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected