title: SC_AT sorted Mph1
organism: Mus musculus
description: Protocols: scAT macrophages were sorted based on CD45+, F4/80+ and CD11b+. AT-macrophages derived from 3D cultures and BM- cultured macrophages were harvested after 13 days in culture.    RNA were extracted from sorted sc-AT macrophages, AT and BM cultured macrophages.  and purified using a Promega extraction kit (Relia PreP ™ RNA Cell Miniprep).  cDNA libraries were generated according to Illumina Stranded mRNA Prep kit (Illumina) from 100ng tRNA. cDNA was amplified for 13 cycles and barcoded with IDT® for Illumina® RNA UD Indexes Set A, Ligation
attributes.tag: ENA first public
attributes.value: 2024-06-24
attributes.tag: INSDC center name
attributes.value: RESTORE
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14120:SC_AT sorted Mph1
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: macrophage
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth condition
attributes.value: in vivo scAT
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: subcutaneous adipose tissue
attributes.tag: sample name
attributes.value: E-MTAB-14120:SC_AT sorted Mph1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57Bl6/J