title: NB29 1A
organism: Hordeum vulgare
description: Barley (Hordeum vulgare cv. RGT Planet) grown in glasshouse for three weeks before spot inoculation with NB29 isolate Pyrenophora teres f. teres (Beecher virulent pathotype; collected Wongan Hills, WA, 1985) spore solution (10 uL droplet, 2000 spores/mL). Sample comprised of total RNA extracted from 24 (2 mm) biopsy punches taken from leaf tissue. 24 punches were made up of 3 punches taken from two observed disease lesions 10-15 cm from the tip of the second leaf from 4 leaves, each from a different plant.
attributes.tag: isolate
attributes.value: NB29
attributes.tag: cultivar
attributes.value: RGT Planet
attributes.tag: age
attributes.value: 3 weeks
attributes.tag: collection_date
attributes.value: 2024-05-31
attributes.tag: geo_loc_name
attributes.value: Australia: Bentley
attributes.tag: tissue
attributes.value: leaf
attributes.tag: collected_by
attributes.value: Jade Davis
attributes.tag: disease
attributes.value: Net form net blotch
attributes.tag: disease_stage
attributes.value: 3 days post innoculation
attributes.tag: isolation_source
attributes.value: Curtin University glasshouse
attributes.tag: lat_lon
attributes.value: 32.010957 S 115.890222 E
attributes.tag: sample_type
attributes.value: leaf tissue
attributes.tag: treatment
attributes.value: NB29 inoculated
attributes.tag: replicate
attributes.value: 1
attributes.tag: BioSampleModel
attributes.value: Plant