title: ELE30_2
organism: Homo sapiens
description: Protocols: Cells were detached with Accutase, counted and crosslinked with 1% (final) formaldehyde in their medium. Reaction was quenched with 0.125M (final) glycine, cell pellets were washed with PBS, snap frozen and stored in -80 freezer. Pellet of 3 million cells was used for H3K27A ChIP experiment. iAstrocytes were grown on hESc-qualified Matrigel (Corning)-coated plates, in Astrocyte Medium (ScienCell). Seeding density was 15,000 cells/sq. cm. ChIP prodecure was performed as described before (Pekowska et al., 2018, Cell). Libraries were constructed using Ovation Ultra System V2 (Tecan, 0344NB-32), according to manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2025-03-20
attributes.tag: External Id
attributes.value: SAMEA114263704
attributes.tag: INSDC center name
attributes.value: Nencki Institute of Experimental Biology
attributes.tag: INSDC last update
attributes.value: 2023-08-14T12:28:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13254:ELE30_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: ELE30
attributes.tag: cell_type
attributes.value: astrocyte
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: juvenile stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: skin
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: sample name
attributes.value: E-MTAB-13254:ELE30_2
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female