title: SD10_18S
organism: marine metagenome
description: eukaryote samples from pelagic particles < 100 µ
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-22
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-22
attributes.tag: External Id
attributes.value: SAMEA110070172
attributes.tag: INSDC center alias
attributes.value: Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research
attributes.tag: INSDC center name
attributes.value: Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research
attributes.tag: INSDC first public
attributes.value: 2022-09-22T00:40:55Z
attributes.tag: INSDC last update
attributes.value: 2022-09-22T00:40:55Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 64118:af2aec02-1230-4034-bab5-bd0d1a426e41
attributes.tag: broker name
attributes.value: German Federation for Biological Data (GFBio)
attributes.tag: collection date
attributes.value: 2018-08-29
attributes.tag: environment (biome)
attributes.value: marine biome [ENVO:00000447] | polar biome [ENVO:01000339]
attributes.tag: environment (feature)
attributes.value: microbial feature [ENVO:01000007] | particulate matter in a hydrosphere [ENVO:01001651]
attributes.tag: environment (material)
attributes.value: sea water [ENVO:00002149]
attributes.tag: environmental package
attributes.value: water
attributes.tag: geographic location (country and/or sea)
attributes.value: Arctic Ocean
attributes.tag: geographic location (depth)
attributes.value: 32.5
attributes.tag: geographic location (latitude)
attributes.value: 77.892
attributes.tag: geographic location (longitude)
attributes.value: 115.958
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: library_construction_protocol
attributes.value: DNA extraction Qiagen DNeasy PowerWater Kit. libraries: 18S rRNA primer were chosen in accordance with the Earth Microbiome Project (https://earthmicrobiome.org/) Primer: TA- Reuk454FWD1 (5'-CCAGCA(G/C)C(C/T)GCGG- TAATTCC-3') and TAReukREV3 (5'-ACTTTCGTTCTTGAT(C/T)(A/G)A-3') (Stoeck et al. 2010; doi: 10.1111/j.1365-294X.2009.04480.x)
attributes.tag: project name
attributes.value: ARCTIC2018
attributes.tag: sample name
attributes.value: 64118:af2aec02-1230-4034-bab5-bd0d1a426e41
attributes.tag: sample_description
attributes.value: eukaryote samples from pelagic particles < 100 µ
attributes.tag: sequencing method
attributes.value: Illumina MiSeq
attributes.tag: submitted to insdc
attributes.value: true
attributes.tag: water environmental package
attributes.value: water