title: Fig7_ACM_DIV3_N1
organism: Rattus norvegicus
description: Protocols: Primary cell cultures of cortical neurons and cortical astrocytes were generated from tissue collected from embryonic day 17.5 CD1 mice and embryonic day 20.5 Sprague-Dawley rats. RNA extraction was carried out using the High Pure RNA Tissue kit (Roche) as per manufacturer's instructions. Barcoded RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer's instructions.
attributes.tag: strain
attributes.value: Sprague-Dawley
attributes.tag: individual
attributes.value: not applicable
attributes.tag: organism
attributes.value: Rattus norvegicus
attributes.tag: organism
attributes.value: Rattus norvegicus
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: not applicable
attributes.tag: stimulus
attributes.value: none
attributes.tag: scientific_name
attributes.value: Rattus norvegicus
attributes.tag: common name
attributes.value: Norway rat
attributes.tag: organism part
attributes.value: brain
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: growth condition
attributes.value: astrocyte-conditioned media
attributes.tag: age
attributes.value: E20.5
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected