title: EMOBON_PiEGetxo_Wa_51
organism: marine plankton metagenome
description: EMOBON metagenome water sample from station PiEGetxo collected on 2021-12-22 size fraction 0.2-3um
attributes.tag: ENA first public
attributes.value: 2025-04-24
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00213
attributes.tag: amount or size of sample collected
attributes.value: 8.5
attributes.tag: broad-scale environmental context
attributes.value: marine biome [ENVO:00000447];estuarine biome [ENVO:01000020]
attributes.tag: chlorophyll
attributes.value: 0.24807
attributes.tag: chlorophyll method
attributes.value: HPLC (Alliance HPLC System) following the method described by Zapata et al. 2000
attributes.tag: collection date
attributes.value: 2021-12-22
attributes.tag: conductivity
attributes.value: 21.8465
attributes.tag: conductivity method
attributes.value: EXO2 multiparameter sonde
attributes.tag: depth
attributes.value: 1
attributes.tag: dna concentration
attributes.value: 19.6
attributes.tag: environmental medium
attributes.value: seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
attributes.tag: geographic location (country and/or sea)
attributes.value: Spain
attributes.tag: geographic location (latitude)
attributes.value: 43.338583
attributes.tag: geographic location (locality)
attributes.value: Abra de Bilbao
attributes.tag: geographic location (longitude)
attributes.value: -3.014639
attributes.tag: geographic location (ocean)
attributes.value: North Atlantic Ocean
attributes.tag: geographic location (region and locality)
attributes.value: Bay of Biscay
attributes.tag: library reads sequenced
attributes.value: 55335149
attributes.tag: local environmental context
attributes.value: marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];harbor[ENVO:00000463]
attributes.tag: mock_eu
attributes.value: DDC_AAABOSDA_2_HVWGWDSX5.UDI143;DDC_AAABOSDA_2_HVWGWDSX5.UDI217
attributes.tag: mock_pr
attributes.value: DDC_AAACOSDA_1_HVWGWDSX5.UDI164;DDC_AAACOSDA_1_HVWGWDSX5.UDI218
attributes.tag: nucleic acid amplification
attributes.value: metag
attributes.tag: nucleic acid extraction
attributes.value: https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
attributes.tag: obs_id
attributes.value: PiEGetxo
attributes.tag: pH
attributes.value: 7.19
attributes.tag: pcr conditions
attributes.value: DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
attributes.tag: pcr primers
attributes.value: metag
attributes.tag: ph method
attributes.value: EXO2 multiparameter sonde
attributes.tag: pigments
attributes.value: Aloxanthin: 0.006728683;Anteraxanthin: 0;B-carotene: 0;Bfu: 0;Clorofyll b: 0.076512581;clorophyll C1: 0;clorophyll C2: 0;Crocoxanthin: 0;Diadinoxanthin: 0;Diatoxanthin: 0;Dinoxanthin: 0;Fucoxanthin: 0.014319685;Hfu: 0;Lutein: 0.021582984;Neoxanthin: 0;Peridinin: 0;Prasinoxanthin: 0;Violaxanthin: 0;Zeaxanthin: 0.014383848
attributes.tag: pigments method
attributes.value: HPLC (Alliance HPLC System) following the method described by Zapata et al. 2000
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: salinity
attributes.value: 18.27
attributes.tag: salinity method
attributes.value: EXO2 multiparameter sonde
attributes.tag: sample collection device
attributes.value: Niskin
attributes.tag: sample material processing
attributes.value: WaSOP 1
attributes.tag: sample name
attributes.value: EMOBON00213
attributes.tag: sample storage location
attributes.value: BBEBB, freezer 12
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: marine plankton metagenome
attributes.tag: sea subsurface temperature
attributes.value: 11.35
attributes.tag: sea subsurface temperature method
attributes.value: EXO2 multiparameter sonde
attributes.tag: sea surface temperature
attributes.value: 11.35
attributes.tag: sea surface temperature method
attributes.value: EXO2 multiparameter sonde
attributes.tag: sequence quality check
attributes.value: manual
attributes.tag: sequence_batch
attributes.value: Batch2
attributes.tag: sequence_batch_rundate
attributes.value: Batch2_230428
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: size-fraction lower threshold
attributes.value: 0.2
attributes.tag: size-fraction upper threshold
attributes.value: 3
attributes.tag: source material identifiers
attributes.value: EMOBON_PiEGetxo_Wa_211222_0.2um_2
attributes.tag: status
attributes.value: sample
attributes.tag: subsurface salinity
attributes.value: 18.27
attributes.tag: subsurface salinity method
attributes.value: EXO2 multiparameter sonde
attributes.tag: target gene
attributes.value: metag
attributes.tag: target subfragment
attributes.value: metag
attributes.tag: tidal stage
attributes.value: high
attributes.tag: tidal stage information
attributes.value: high_tide
attributes.tag: total depth of water column
attributes.value: 10
attributes.tag: turbidity
attributes.value: 0