title: Model organism or animal sample from Hemibagrus filamentus
organism: Hemibagrus filamentus
description: This project involves the assembly of the complete mitochondrial DNA genome of Hemibagrus filamentus. Muscle tissue samples were used for total DNA extraction. The entire mtDNA sequence was amplified by two PCR reactions from the total DNA sample. Each PCR product of 9000bp was purified from the agarose gel and then size selected before being mixed together in a 1:1 ratio. The mixture of the two PCR products was further processed with the Rapid Sequencing Kit V14 - gDNA (SQK-RAD114) and then sequenced on the MinION Mk1D portable sequencer
attributes.tag: isolate
attributes.value: HCM
attributes.tag: age
attributes.value: 2 months
attributes.tag: collection_date
attributes.value: 2025-05-20
attributes.tag: geo_loc_name
attributes.value: missing
attributes.tag: sex
attributes.value: not applicable
attributes.tag: tissue
attributes.value: muscle
attributes.tag: BioSampleModel
attributes.value: Model organism or animal