title: High-fat diet and Vehicle (HFD+Vehicle)
organism: Mus musculus
description: We use the high-fat diet (HFD) to build obese model in mice and evaluate effects of our FKBP51-Hsp90 interaction inhibitor (E8). There are four groups: Control diet (CD), HFD, CD + E8, and HFD+E8. We isolated total RNA from the hippocampus (n=5/group, 20 in total) using RNeasy Lipid Tissue Mini Kit (Cat. No. 74804, Qiagen, Germany) according to its handbook. RNA concentration and quality were assessed using fluorometric measurements on the Qubit 3.0 instrument (ThermoFisher Scientific) and capillary electrophoresis (Caliper GX II, Agilent), respectively. Sample ID in four groups: HFD+E8 (P24807_101 to P24807_105); CD+E8 (P24807_106 to P24807_110); HFD+Vehicle (P24807_111 to P24807_115); CD+Vehicle (P24807_116 to P24807_120).
attributes.tag: ENA first public
attributes.value: 2024-06-15
attributes.tag: External Id
attributes.value: SAMEA110132319
attributes.tag: INSDC center name
attributes.value: Pavel Pavlov
attributes.tag: INSDC last update
attributes.value: 2022-06-15T15:19:41Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: HFD-54_P24807_113
attributes.tag: collected_by
attributes.value: Lisha Wang, Jakub Wojcieszak
attributes.tag: collection_date
attributes.value: 2021-10-15
attributes.tag: common name
attributes.value: house mouse
attributes.tag: geographic location (country and/or sea)
attributes.value: Sweden
attributes.tag: geographic location (region and locality)
attributes.value: Solna
attributes.tag: isolation_source
attributes.value: Hippocampus
attributes.tag: lab_host
attributes.value: Janvier Labs
attributes.tag: sample name
attributes.value: HFD-54_P24807_113
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J
attributes.tag: tissue_type
attributes.value: Mouse hippocampus