title: Sample 5
organism: Mus musculus
description: Protocols: Euthanized animals were perfused with 20 ml of PBS before hearts were harvested. Mice were treated i.p. twice per week with 200 µg of 14-D10-2 anti-Gremlin monoclonal antibodies or IgG2b isotype control. For enzymatic dissociation hearts were minced into small pieces and were placed into a six-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (Lonza), 1 mg/ml collagenase D (Sigma), and 25 μg/ml DNase I (Applichem) and incubated at 37°C under continuous stirring. The remaining tissue pieces were mechanically disrupted. Nuclei were isolated from frozen murine hearts and sorted to enrich intact nuclei using a BDFACSMelody. Sorted single nuclei suspensions were emulsified for library generation using the droplet-based 10x Chromium (10x Genomics) system. cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3' Reagent Kit (v3 Chemistry).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-01-17T16:00:45Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-01-17T16:00:45Z
attributes.tag: External Id
attributes.value: SAMEA112352779
attributes.tag: INSDC center name
attributes.value: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
attributes.tag: INSDC first public
attributes.value: 2024-01-17T16:00:45Z
attributes.tag: INSDC last update
attributes.value: 2024-01-17T16:00:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12559:Sample 5
attributes.tag: age
attributes.value: 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: myocarditis
attributes.tag: genotype
attributes.value: MYH6-specific TCR transgene
attributes.tag: individual
attributes.value: mouse 5
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: heart
attributes.tag: sample name
attributes.value: E-MTAB-12559:Sample 5
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: BALB/c