title: Tomato rizosphere inoculated by Pseudomonas putida KT2440 K2K3-T6SS-mutants
organism: rhizosphere metagenome
description: Tomato rizosphere inoculated by Pseudomonas putida KT2440 K2K3-T6SS-mutants
attributes.tag: host
attributes.value: tomato
attributes.tag: isolation_source
attributes.value: soil
attributes.tag: collection_date
attributes.value: 2021-06
attributes.tag: geo_loc_name
attributes.value: Spain:Madrid
attributes.tag: lat_lon
attributes.value: 40.543224 N 3.692159 W
attributes.tag: samp_mat_process
attributes.value: Soil DNA was extracted using the FastDNA Spin Kit for Soil (MP Biomedicals, USA), following the manufacturers protocol with additional modifications. For bacterial 16S rRNA profiling, the extracted soil DNA samples were sent to Macrogen. The V3-V4 regions of the 16S rRNA gene were amplified using Illumina MiSeq paired-end 2x300 system with primers 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC)
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental