title: NS_IAA_AID_KI_CTCF-AID-GFP
organism: Mus musculus
description: Protocols: Cells were detached with Accutase, counted and 100,000 cells were immediately used for the ATAC-seq experiments. ES (2i/LIF) cells were grown on gelatin-coated culture plastic in embryonic stem cells medium in the presence of MEK inhibitor (PD0325901) and GSK3 inhibition (CHIR99021) with LIF to maintain ESCs in a naive ground state. Cells were split at 80% confluence with a density of 30000 cells/sq.cm. The medium was exchanged daily. NS cells were grown on laminin-coated plates in neural differentiation medium containing N2 and B27 supplements along with recombinant murine EGF and bFGF to a final concentration of 10 ng/ml. Cells were split at 80% confluence with a density of 35000 cells/sq.cm. The medium was exchanged daily. CTCF depletion was carried out in CTCF-AID-GFP ES and NS Cells by treating with 500uM of indole acetic acid (IAA) dissolved in culture media for 24h ATAC-seq procedure was performed with ATAC-Seq kit (Active Motif, 53150), according to manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2024-12-13
attributes.tag: External Id
attributes.value: SAMEA114664842
attributes.tag: INSDC center name
attributes.value: Dioscuri Centre for Chromatin Biology and Epigenomics, Nencki Institute of Experimental Biology, Polish Academy of Sciences
attributes.tag: INSDC last update
attributes.value: 2023-11-22T22:02:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13559:NS_IAA_AID_KI_CTCF-AID-GFP
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: embryonic cell line
attributes.tag: cell_type
attributes.value: neural progenitor cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: inner cell mass
attributes.tag: sample name
attributes.value: E-MTAB-13559:NS_IAA_AID_KI_CTCF-AID-GFP
attributes.tag: scientific_name
attributes.value: Mus musculus