title: C7-CIO-D60-scRNA-seq
organism: Pan troglodytes
description: Protocols: Developing intestinal tissues and organoids samples were enzymatically and mechanically dissociated into single cells using the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628). For scATAC and multiome, nuclei were isolated with a custom lysis buffer for developing human tissue, while 10x Genomics protocols were followed for all other samples. Specific human and chimpanzee organoid single cell suspensions were pooled at equal amount.  For all samples, nucleic acids were extracted and processed following the recommendations and standard protocols of 10x Genomics for the corresponding data type. For all samples, scRNA-seq, scATAC-seq and multiome libraries were generated on a 10x Chromium device following the recommendations and standard protocols of 10x Genomics for the corresponding data type.