title: MeioMeNGS_1
organism: Arabidopsis thaliana
description: Protocols: Liquid nitrogen frozen 15 days-old in vitro culture plants. Arabidopsis seeds were surface sterilized for 10 min in 70% ethanol + 0.05% sodium dodecyl sulfate and washed for 10 min in 70% ethanol and grown in Petri dishes with culture medium (Gamborg B5 medium-Duchefa supplied with 0.16% bromocresol purple and 0.1% sucrose). The generation of GFP-tagged CENH3 (GFP::CENH3) has been described in (Ravi and Chan, 2010) The generation of RFP-tagged REC8 (REC8 :RFP) line in (Kuttig et al., 2022) DNA was prepared using the NucleoSpin Plant II kit (Macherey Nagel) following the manufacturer's instructions. Standard genomic library construction