title: pl4bA12
organism: Mus musculus
description: Protocols: Embryos were generated through IVF, eventually microinjected and then cultured in a standard mammalian cell incubator in KSOM medium. Separation of RNA from single embryos with SPRI beads. Modified Smart-Seq2 protocol using SuperScript IV RT and tagmentation procedure previously described (Hennig et al. 2018).