title: Sample 1
organism: Arabidopsis thaliana
description: Protocols: 7-day old seedlings post-treatment were collected and frozen immediately in liquid nitrogen and stored at -80°C for further processing. Plants were grown under 16h light. Total RNA is extracted using the Trizol method followed by DNase treatment as per the manufacturer's instructions. RNA Integrity is quantified using an Agilent 2100 Bioanalyzer and RNA 6000 pico kit. RNA quality RIN > 8 were selected for library preparation. Libraries are prepared using NEBNext® Ultra II RNA Library Prep Kit for Illumina® as per the manufacturer's protocol with a starting concentration of 2ug.