title: Monkeypox whole-genome sequencing from Netherlands 2022
organism: Monkeypox virus
description: Nanopore sequencing was performed on clinical material after DNAse treatment. In short, DNA was extracted using Ampure Beads after which an end repair step was performed (Ultra II End-prep NEBNext). Adapters were ligated, Ampure bead washes were performed twice and a random PCR was performed (following the SQK-PBK004 protocol from Nanopore)