title: sc_E5_Hep
organism: Homo sapiens
description: Protocols: Human adult liver tissue samples were obtained from macroscopically healthy tissue that remained from resected human liver of patients with primary or secondary liver tumors or benign liver diseases with or without pretreatment with portal vein embolization. Informed consent of the patients for the use of tissue for research purposes was obtained according to the ethical guidelines of Leipzig University Hospital (006/17-ek, 21 March 2017, revised and renewed 12 February 2019). Acquired tissue from portal vein embolized livers included samples of regenerating (R) and embolized (E) tissue, whereas tissue samples from benign liver diseases were defined as quiescent healthy controls (H). Single stranded cDNA was generated in droplets according to 10X Genomics 3' Single cell Kit v2 and v3 guide instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. cDNA libraries were prepared as described in the 10X Genomics user guide (Single Cell 3â€² Reagent Kits User Guide PN-120233, 10X Genomics). Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays