title: Sample 2
organism: Mus musculus
description: Protocols: The Defa6-iCre R26-TdTomato mouse model, which exhibits restricted expression of the TdTomato fluorescent protein in medullary thymic epithelial cells, was used as the recipient for competitive bone marrow chimeras composed of Claudin-1–sufficient and Claudin-1–deficient donor cells. After six weeks, thymi were dissected and type 1 dendritic cells arising from Claudin-1–sufficient and Claudin-1–deficient bone marrow were isolated by flow cytometric sorting from one individual animal. Cells were directly sorted into lysis buffer. Complete mRNA was isolated based on polyA selection using Dynabeads mRNA direct purification kit.  Sequencing libraries were prepared using the MARS-seq protocol, as described previously (Jaitin, D. A. et al. Massively parallel single-cell RNA-seq for marker-free decomposition of tissues into cell types. Science 343, 776–779 (2014)).