title: A8_TR
organism: Mus musculus
description: Protocols: Embryos were generated through IVF, microinjected, cultured in a standard mammalian cell incubator in KSOM medium until 2-cell stage and then transferred to pseudopregnant females for in vivo growth until E7. Extraction of RNA from single E7 embryonic halves using Monarch Total RNA Miniprep Kit (NEB). Modified Smart-Seq2 protocol using SuperScript IV RT and tagmentation procedure previously described (Hennig et al. 2018).