organism: uncultured bacterium
attributes.tag: ENA first public
attributes.value: 2025-06-12
attributes.tag: INSDC center name
attributes.value: EMG
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: SRR18550247_binner12_Refined_17
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaspades_v3.15.5
attributes.tag: binning parameters
attributes.value: default
attributes.tag: binning software
attributes.value: MGnify-genomes-generation-pipeline_v1.0.0
attributes.tag: broad-scale environmental context
attributes.value: maquatic
attributes.tag: broker name
attributes.value: EBI-EMG
attributes.tag: collection date
attributes.value: 2017-06
attributes.tag: completeness score
attributes.value: 90.96
attributes.tag: completeness software
attributes.value: CheckM2_v1.0.1
attributes.tag: contamination score
attributes.value: 0.99
attributes.tag: environmental medium
attributes.value: biofilter substrate
attributes.tag: geographic location (country and/or sea)
attributes.value: USA
attributes.tag: geographic location (latitude)
attributes.value: missing: third party data
attributes.tag: geographic location (longitude)
attributes.value: missing: third party data
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: sediment metagenome
attributes.tag: local environmental context
attributes.value: aquarium system
attributes.tag: metagenomic source
attributes.value: sediment metagenome
attributes.tag: project name
attributes.value: In commercial large-scale aquaria, microorganisms capable of transforming toxic nitrogen species into more benign species play important roles in system health. Many microbes convert nitrite and ammonium into nitrate via nitrification. However, nitrate is toxic to macrofauna at high concentrations. Some bacteria within the phylum Planctomycetes transform ammonium and nitrite to nitrogen gas via anaerobic ammonium oxidation (anammox). Here, we characterized microbial communities in four habitats at the Tennessee Aquarium and examined the abundance and anammox potential of Planctomycetes using culture-independent approaches. 16S rRNA gene amplicon sequencing of habitats revealed distinct, yet stable, microbial communities and the presence of Planctomycetes (~1-15% of each community). Metagenomic analyses identified the genetic potential for multiple complete nitrogen metabolism pathways. However, no known genes diagnostic of the anammox reaction were found in this survey. Given the low relative abundance of these bacteria, a nested Planctomycete-specific 16S rRNA gene-based PCR approach was used to (i) enrich amplicons representative of group members and (ii) identify anammox bacteria based on diagnostic genetic signatures. This amplicon enrichment identified highly diverse sequences that share <95% 16S rRNA gene sequence identity to cultured Planctomycetes, revealing an opportunity to study novel strains within this phylum. These data indicate that putative anammox organisms are present within these exhibits, but further study is required to confirm their capability to perform the anammox reaction.
attributes.tag: sample derived from
attributes.value: SAMN15908822
attributes.tag: sample name
attributes.value: SRR18550247_binner12_Refined_17
attributes.tag: scientific_name
attributes.value: uncultured bacterium
attributes.tag: sequencing method
attributes.value: Illumina MiSeq
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach