organism: Agrobacterium pusense
attributes.tag: strain
attributes.value: KSSKSLAB04
attributes.tag: collection_date
attributes.value: 2022-10-15
attributes.tag: depth
attributes.value: 500X
attributes.tag: elev
attributes.value: 10 m
attributes.tag: env_broad_scale
attributes.value: Coastal marine environment
attributes.tag: env_local_scale
attributes.value: Plastic waste site near Juhu Beach shoreline
attributes.tag: env_medium
attributes.value: Plastic debris
attributes.tag: isolation-source
attributes.value: soil samples polluted with plastic waste
attributes.tag: geo_loc_name
attributes.value: India: Juhu Beach, Mumbai
attributes.tag: isol_growth_condt
attributes.value: The bacterium was isolated from plastic waste collected near Juhu Beach, Mumbai, India. The plastic waste samples were enriched in nutrient broth containing 100 mg/L of plasticizer compounds to select for plasticizer-degrading bacteria. Serial dilution and plating were performed on nutrient agar plates supplemented with plasticizer compounds. Colonies showing visible degradation or clearing zones were selected, purified by repeated streaking, and maintained on nutrient agar slants. The isolate was routinely cultured in nutrient broth at 30 C with shaking at 150 rpm under aerobic conditions.
attributes.tag: lat_lon
attributes.value: 19.1075 N 72.8263 E
attributes.tag: num_replicons
attributes.value: 3
attributes.tag: ref_biomaterial
attributes.value: Bacterial isolate maintained in the Microbiology Laboratory, Eminent Biosciences (EMBS), Indore, Madhya Pradesh, India.
attributes.tag: BioSampleModel
attributes.value: MIGS.ba
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.soil