organism: uncultured bacterium
attributes.tag: ENA first public
attributes.value: 2025-06-12
attributes.tag: INSDC center name
attributes.value: EMG
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: SRR15668623_binner123_Refined_8
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaspades_v3.15.5
attributes.tag: binning parameters
attributes.value: default
attributes.tag: binning software
attributes.value: MGnify-genomes-generation-pipeline_v1.0.0
attributes.tag: broad-scale environmental context
attributes.value: marine
attributes.tag: broker name
attributes.value: EBI-EMG
attributes.tag: collection date
attributes.value: 2013-05-07
attributes.tag: completeness score
attributes.value: 100
attributes.tag: completeness software
attributes.value: CheckM2_v1.0.1
attributes.tag: contamination score
attributes.value: 0.32
attributes.tag: environmental medium
attributes.value: marine sediment
attributes.tag: geographic location (country and/or sea)
attributes.value: Pacific Ocean
attributes.tag: geographic location (latitude)
attributes.value: 33.78898000
attributes.tag: geographic location (longitude)
attributes.value: -118.66795800
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: sediment metagenome
attributes.tag: local environmental context
attributes.value: methane seep sediments
attributes.tag: metagenomic source
attributes.value: sediment metagenome
attributes.tag: project name
attributes.value: Biological anaerobic oxidation of methane (AOM) coupled to sulfate reduction represents a large methane sink in global ocean sediments. Methane consumption is carried out by syntrophic archaeal-bacterial consortia and fuels an unique ecosystem, yet these slow growing interactions in the syntrophic consortia and between the associated community members remain poorly understood. We established in vitro enrichments performing AOM with sulfate by selecting and cultivating microbes of interest based on size, density and metabolism. By analysing community composition of the enrichments and single aggregates, we shed light on the microbial assemblage that thrive on methane and could be physically associated together. These in vitro enrichments will serve as the model system to study microbial interactions in marine methane seeps.
attributes.tag: sample derived from
attributes.value: SAMN21037847
attributes.tag: sample name
attributes.value: SRR15668623_binner123_Refined_8
attributes.tag: scientific_name
attributes.value: uncultured bacterium
attributes.tag: sequencing method
attributes.value: Illumina HiSeq 2500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach