organism: respiratory tract metagenome
attributes.tag: ENA first public
attributes.value: 2024-08-07
attributes.tag: FEV1
attributes.value: 112
attributes.tag: INSDC center name
attributes.value: IMU
attributes.tag: INSDC status
attributes.value: public
attributes.tag: LCI
attributes.value: 8.8
attributes.tag: Submitter Id
attributes.value: MU01_V05_EXP
attributes.tag: broad-scale environmental context
attributes.value: Host-associated
attributes.tag: broad-scale environmental context
attributes.value: human-associated
attributes.tag: chimera check software
attributes.value: zAMP
attributes.tag: collection date
attributes.value: 2018-02-07
attributes.tag: cpneumoniae at visit
attributes.value: No
attributes.tag: environmental medium
attributes.value: respiratory system
attributes.tag: environmental medium
attributes.value: Sputum
attributes.tag: geographic location (country and/or sea)
attributes.value: Switzerland
attributes.tag: geographic location (latitude)
attributes.value: 46.52522
attributes.tag: geographic location (longitude)
attributes.value: 6.64245
attributes.tag: host age
attributes.value: 14
attributes.tag: host sex
attributes.value: male
attributes.tag: host subject id
attributes.value: MU01
attributes.tag: investigation type
attributes.value: amplicon sequencing
attributes.tag: library construction method
attributes.value: paired-end
attributes.tag: library screening strategy
attributes.value: PCR
attributes.tag: library yield
attributes.value: 215
attributes.tag: local environmental context
attributes.value: Human
attributes.tag: local environmental context
attributes.value: Lungs
attributes.tag: lung/pulmonary disorder
attributes.value: Cystic fibrosis
attributes.tag: mpneumoniae at visit
attributes.value: No
attributes.tag: nucleic acid amplification
attributes.value: 16S Metagenomic Sequencing Library Preparation (Part. #15044223 Rev. B), Illumina (San Diego, United States)
attributes.tag: nucleic acid extraction
attributes.value: MagNA Pure 96 Roche (Basel, Switzerland)
attributes.tag: pcr conditions
attributes.value: initial denaturation:95degC_5min; denaturation:95degC_0.5min; annealing:55degC_0.5min; elongation:72degC_0.5min; final elongation:72degC_5min; total cycles:25
attributes.tag: pcr primers
attributes.value: FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC
attributes.tag: project name
attributes.value: MUCOVIB
attributes.tag: project title
attributes.value: Unexpected associations between respiratory viruses and bacteria with Pulmonary Function Testing in children suffering from Cystic Fibrosis (MUCOVIB study)
attributes.tag: sample name
attributes.value: MU01_V05_EXP
attributes.tag: scientific_name
attributes.value: respiratory tract metagenome
attributes.tag: sequencing method
attributes.value: Illumina MiSeq
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V3V4
attributes.tag: visit status
attributes.value: Regular visit