organism: Homo sapiens
attributes.tag: ENA first public
attributes.value: 2025-06-27
attributes.tag: INSDC center name
attributes.value: BioISI - Faculty of Sciences, University of Lisbon
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: D1A34
attributes.tag: Treatment
attributes.value: Peripheral blood mononuclear cells (PBMC) were isolated from buffy coats by density gradient centrifugation using Lymphosep (Biowest), followed by isolation of naive CD4+T cells by negative selection using the MojoSort� Human CD4 Na�ve T Cell Isolation Kit (BioLegend). Naive CD4+ T cells were stimulated for 40 hours with CD3/CD28 dynabeads (Gibco) (1bead:10 cells) + 10U/mL of hrIL-2. After 40 hours, stimulation beads were removed, cells were washed with phosphate buffered saline (PBS) and resuspended in serum-free RPMI to reach a final concentration of 2 million cells/mL/well and plated on a 24-well plate. Control or miR-34c-5p-specific-antagomiRs were directly added to the plated cells to reach a final concentration of 1�M. AntagomiR-treated cells were incubated for 2 hours. After treatment, the medium was replaced, and cells were cultured in RPMI-1640 + GlutaMAX (Gibco) medium supplemented with 10% FBS, 1% nonessential amino acids, 1%, sodium pyruvate, 1% penicillin/streptomycin, 0.1% gentamycin and 10U/mL of hrIL-2for 100 hours more. Total RNA was extracted with miRNeasy Mini Kit (Qiagen).
attributes.tag: cell_type
attributes.value: Naive CD4+ T cells
attributes.tag: collection date
attributes.value: 2022-08
attributes.tag: common name
attributes.value: human
attributes.tag: geographic location (country and/or sea)
attributes.value: Portugal
attributes.tag: sample name
attributes.value: D1A34
attributes.tag: scientific_name
attributes.value: Homo sapiens