organism: Homo sapiens
attributes.tag: isolate
attributes.value: Genomic DNA was isolated from FACS-isolated cells using phenol extraction. Sequences of guide RNAs were amplified from genomic DNA in two steps. First, a linear sgRNA amplification was performed using Herculase II DNA polymerase using 13 thermal cycles with a single sgRNA promoter-specific primer, RandomF (sequences of all primers are listed in Supplemental Data S4), and the following cycling parameters: 96C 20s, 63C 1min, 72C 90s. Then, the second PCR primer, RandomR, was added to the reaction and a regular PCR was performed for 35 cycles as follows: 96C 3min; 35 cycles of 96C 20s, 63C 1min, 72C 45s; 72C 10min; 4C.
attributes.tag: age
attributes.value: Not applicable
attributes.tag: biomaterial_provider
attributes.value: Andrei Alexandrov
attributes.tag: collection_date
attributes.value: 2017-10-18
attributes.tag: geo_loc_name
attributes.value: Not applicable
attributes.tag: sex
attributes.value: Not applicable
attributes.tag: tissue
attributes.value: Not applicable
attributes.tag: cell_line
attributes.value: Hela cell
attributes.tag: BioSampleModel
attributes.value: Human