organism: rhizosphere metagenome
attributes.tag: ENA first public
attributes.value: 2024-02-26
attributes.tag: External Id
attributes.value: SAMEA114790128
attributes.tag: INSDC center name
attributes.value: TUBS-IPB
attributes.tag: INSDC last update
attributes.value: 2023-12-05T10:45:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: V9_7_Col_2
attributes.tag: broad-scale environmental context
attributes.value: controlled environment (climate chamber)
attributes.tag: collection date
attributes.value: 2020
attributes.tag: environmental medium
attributes.value: soil (artificial substrate mixture of potting compost, sand, vermiculite, fertilizer)
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: 52
attributes.tag: geographic location (longitude)
attributes.value: 10
attributes.tag: host age
attributes.value: 6
attributes.tag: host common name
attributes.value: Arabidopsis thaliana
attributes.tag: host genotype
attributes.value: Col-0 wildtype
attributes.tag: host taxid
attributes.value: 3702
attributes.tag: local environmental context
attributes.value: mesocosm pot experiment planted with Arabidopsis thaliana
attributes.tag: multiplex identifiers
attributes.value: GGCTAC,ATGAGC
attributes.tag: nucleic acid extraction
attributes.value: Fast DNA SpinKit for Soil, MP Biomedicals, Germany
attributes.tag: pcr primers
attributes.value: CCTAYGGGRBGCASCAG,GGACTACNNGGGTATCTAAT
attributes.tag: plant product
attributes.value: rhizosphere
attributes.tag: project name
attributes.value: Amplicon sequencing of 16S rRNA gene fragments of Arabidopsis Rhizosphere
attributes.tag: sample collection method
attributes.value: vigurously shaking root cluster, removing roots by vortexing, centrifuging to obtain rhizsophere pellet
attributes.tag: sample name
attributes.value: V9_7_Col_2
attributes.tag: scientific_name
attributes.value: rhizosphere metagenome
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V3-V4 on 16S rRNA