organism: soil metagenome
attributes.tag: organism
attributes.value: soil metagenome
attributes.tag: broad-scale environmental context
attributes.value: controlled environment (climate chamber)
attributes.tag: collection date
attributes.value: 2020
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: geographic location (longitude)
attributes.value: 10
attributes.tag: environmental medium
attributes.value: soil (artificial substrate mixture of potting compost, sand, vermiculite, fertilizer)
attributes.tag: geographic location (latitude)
attributes.value: 52
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sample collection method
attributes.value: homogenizing substrate mixture by stirring, sampling approximately 1.5 g
attributes.tag: target subfragment
attributes.value: V3-V4 on 16S rRNA
attributes.tag: pcr primers
attributes.value: CCTAYGGGRBGCASCAG,GGACTACNNGGGTATCTAAT
attributes.tag: local environmental context
attributes.value: mesocosm pot experiment
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-02-26T16:08:26Z
attributes.tag: project name
attributes.value: Amplicon sequencing of 16S rRNA gene fragments of Arabidopsis Rhizosphere
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-02-26T16:08:26Z
attributes.tag: nucleic acid extraction
attributes.value: Fast DNA SpinKit for Soil, MP Biomedicals, Germany
attributes.tag: multiplex identifiers
attributes.value: TGACCA,CAAAAG
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-02-26
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-02-26