title: RH13
organism: Caenorhabditis elegans
description: Protocols: For RNA isolation, all RNa collection was performed at day 2 of adulthood. ~1000 animals were harvested from RNAi plates using M9. Animals were pelleted by gravity by allowing adult worms to settle to the bottom of the tube and aspirating off eggs and L1. Animals were washed and gravity settled 3x to remove a majority of progeny, then animals were placed into Trizol solution and worms were freeze/thawed 3x with liquid nitrogen with a 30 sec vortexing step between each freeze cycle.  Standard bleaching protocol was used to synchronize animal populations. Animals were L1 arrested for a maximum of 16 hours to further synchronize populations. Animals were grown on RNAi indicated from L1 until Day 1 of adulthood and material was collected on Day 1.  After the final thaw, chloroform was added at a 1:5 chlorform/trizol ratio and aqueous separation of RNA was performed via centrifugation in a heavy gel phase-lock tube (VWR, 10847-802). The aqueous phase was mixed 1:1 with isopropanol then applied to a Qiagen RNeasy Mini Kit (74106) and RNA purification was performed as per manufacturer's directions.  Library construction was performed by Novogene using their standard pipeline.
attributes.tag: ENA first public
attributes.value: 2025-08-31
attributes.tag: INSDC center name
attributes.value: Leonard Davis School of Gerontology University of Southern California
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15031:RH13
attributes.tag: age
attributes.value: 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: glp-4(bn2) I;
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-15031:RH13
attributes.tag: scientific_name
attributes.value: Caenorhabditis elegans
attributes.tag: sex
attributes.value: hermaphrodite
attributes.tag: strain
attributes.value: glp-4 (bn2)