title: sample_30_rep1
organism: Mus musculus
description: Protocols: Pancreatic tissue was collected from 8-12 week old mice Acute pancreatitis was induced as published (Algül et al., JCI 2007), Buthionine sulphoximine (BSO) was given at 20mM in the drinking water  for 48 hours before AP induction Maxwell 16 Tissue LEV Total RNA Purification Kit was used for RNA extraction according to the manufacturer's instructions (Promega) Library preparation for bulk 3'-sequencing of poly(A)-RNA was done as described previously (Parekh et al., 2016) barcoded cDNA of each sample was generated with a Maxima RT polymerase (Thermo Fisher) using oligo-dT primer containing barcodes, unique molecular identifiers (UMIs) and an adapter. 5' ends of the cDNAs were extended by a template switch oligo (TSO) and after pooling of all samples full-length cDNA was amplified with primers binding to the TSO-site and the adapter. cDNA was tagmented with the Nextera XT kit (Illumina) and 3'-end-fragments finally amplified using primers with Illumina P5 and P7 overhangs.
attributes.tag: ENA first public
attributes.value: 2025-08-30
attributes.tag: INSDC center name
attributes.value: Klinik und Poliklinik für Innere Medizin II TUM Klinikum Rechts der Isar
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15511:sample_30_rep1
attributes.tag: age
attributes.value: 8-12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Control
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: pancreas
attributes.tag: sample name
attributes.value: E-MTAB-15511:sample_30_rep1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: stimulus
attributes.value: BSO+AP
attributes.tag: strain
attributes.value: C57BL6 / FVB/129