title: P0_CD133_RGC_Droplet_rep
organism: Mus musculus
description: Protocols: Dissection of VZs from neonatal mouse brains was performed as described 1. Briefly, the midbrain, the olfactory bulb, meninges and the hippocampus were removed in sequence to attain the telencephalon. Then VZ-containing tissues were separated from the telencephalon. Tissues from 4 mice were pooled, transferred to a 1.5ml Eppendorf tube, and digested with freshly prepared 10U/ml Papain solution (Worthington, catalog no. LS003126) at 37°C for 30 minutes. Then the Papain solution was aspirated, and 1ml culture medium (20% FBS) was added, followed by 1 minute of incubation to terminate the digestion. The supernatant was removed, and another 1ml culture medium (20% FBS) was added. The digested tissues were gently pipetted up and down by 20 times with a P1000 tip to dissociate the cells mechanically. Centrifuge at 350rcf for 5 minutes and discard the supernatant. DNA was purified first by Qiagen minElute PCR purification kit according to the manufacturer's instruction, then by AmupureXP beads with 0.5X upper cutoff and a 1.2X lower cutoff according to manufacturer's instruction. Libraries were constructed using the 10x Genomics Gene Expression 3' kit according to the manual.
attributes.tag: ENA first public
attributes.value: 2025-05-30
attributes.tag: INSDC center name
attributes.value: Southern University of Science and Technology
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13858_2:P0_CD133_RGC_Droplet_rep
attributes.tag: age
attributes.value: 0
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: P0 CD133 glial cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: radial glia
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: ventricular zone
attributes.tag: sample name
attributes.value: E-MTAB-13858_2:P0_CD133_RGC_Droplet_rep
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: single-cell platform
attributes.value: 10x Genomics Gene Expression 3'
attributes.tag: strain
attributes.value: C57BL/6Jax