title: MIGS Eukaryotic sample from Paralagenidium karlingii
organism: Paralagenidium karlingii
description: Paralagenidium karlingii is the causative agent of paralagenidiosis in dogs and cats. This organism often promotes cutaneous restricted lesions that resemble infections caused by P. insidiosum and Lagenidium giganteum forma caninum, although it is more responsive to therapy. Our research focus is to investigate evolutionary, functional, and diagnostic insights through a comparative genomic analysis across major mammalian pathogenic oomycetes, including genomic data from P.karlingii. Paralagenidium karlingii strain was isolated from a dog presenting clinical lesions compatible with paralagenidiosis. Identification of the organism was performed by microbiological culture and homology analysis of rDNA sequence. Genome sequencing was carried out on the resultant gDNA utilizing combination of the Illumina MiSeq platform, and MinION by Oxford Nanopore Technologies, folowed by de Novo assembly and annotation.
attributes.tag: isolate
attributes.value: 2410218
attributes.tag: collection_date
attributes.value: 2024-09-17
attributes.tag: env_broad_scale
attributes.value: host-associated
attributes.tag: env_local_scale
attributes.value: Canis lupus familiaris
attributes.tag: env_medium
attributes.value: room temperature in sterile container
attributes.tag: geo_loc_name
attributes.value: USA:Virginia
attributes.tag: host
attributes.value: Canis lupus familiaris
attributes.tag: isol_growth_condt
attributes.value: Columbia blood agar, incubation at 35C for 48 to 72 hours
attributes.tag: lat_lon
attributes.value: missing
attributes.tag: host_age
attributes.value: 2 years
attributes.tag: host_common_name
attributes.value: Great Pyrenees
attributes.tag: host_sex
attributes.value: male
attributes.tag: host_tissue_sampled
attributes.value: skin
attributes.tag: BioSampleModel
attributes.value: MIGS.eu
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated