title: Soil sample B2_T7
organism: soil metagenome
description: Soil from plot with no biochar and willow
attributes.tag: ENA first public
attributes.value: 2026-04-18
attributes.tag: INSDC center name
attributes.value: CHALMERS
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: B2_T7
attributes.tag: broad-scale environmental context
attributes.value: terrestrial biome
attributes.tag: collection date
attributes.value: 2023-09-21
attributes.tag: depth
attributes.value: 50-250
attributes.tag: elevation
attributes.value: 0
attributes.tag: environmental medium
attributes.value: soil
attributes.tag: geographic location (country and/or sea)
attributes.value: Sweden
attributes.tag: geographic location (latitude)
attributes.value: 56.0667
attributes.tag: geographic location (longitude)
attributes.value: 13.2667
attributes.tag: local environmental context
attributes.value: agricultural soil
attributes.tag: pcr conditions
attributes.value: Initial denaturation 95C 3 min; 20 cycles of 98C 20 s, 57C 30 s, 72C 75 s; final extension 72C 5 min.
attributes.tag: pcr primers
attributes.value: 27F (AGRGTTYGATYMTGGCTCAG) / 1492R (RGYTACCTTGTTACGACTT)
attributes.tag: project name
attributes.value: DDT contaminated soil microbiome
attributes.tag: sample collection device
attributes.value: Core sampler
attributes.tag: sample name
attributes.value: B2_T7
attributes.tag: sample storage conditions
attributes.value: Freezing at -80C
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: target gene
attributes.value: 16S rRNA