title: M Ribo
organism: Mus musculus
description: Protocols: iBMDM were infected with WT or ΔprgJ Salmonella Typhimurium or mock infected for 60 min. Cells were lysed in buffer containing cycloheximide and chloramphenicol, and lysates were split for RNA-Seq and Ribo-Seq. For Ribo-Seq, lysates were treated with RNase I and fragments protected from digestion by the ribosome were purified. For RNA-Seq, total cellular RNA was fragmented by alkaline hydrolysis. Both produce short RNA fragments, from which libraries were generated.
attributes.tag: ENA first public
attributes.value: 2025-08-29
attributes.tag: INSDC center name
attributes.value: University of Cambridge Department of Pathology
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15534:M Ribo
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: iBMDM
attributes.tag: cell type
attributes.value: macrophage
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: infect
attributes.value: Mock
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: Bone marrow derrived macrophage
attributes.tag: sample name
attributes.value: E-MTAB-15534:M Ribo
attributes.tag: scientific_name
attributes.value: Mus musculus