title: sh_HNRNPA2B1_1
organism: Homo sapiens
description: Protocols: KYSE150 cells (CL-0638, Procell Life Science & Technology Co., Ltd., China) were cultured at 37℃ with 5% CO2 in an RPMI-1640 and Ham's F-12 (PM150110 and PM150810, Procell Life Science & Technology Co., Ltd., China) with 10% fetal bovine serum (FBS) (10091148, Gibco, China), 100 µg/mL streptomycin, 100 U/mL penicillin (SV30010, Hyclone, USA). Cells were infected by lentivirus with MOI=50. Stable cell lines were obtained by screening with 1 µg/mL puromycin and cells were harvested for RT-qPCR and Western blotting analysis 48-hours post-transfection, total RNA was extracted using TRIzol reagent (Ambion). VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme) was used to prepare strand-specific RNA-seq libraries.
attributes.tag: ENA first public
attributes.value: 2026-01-09
attributes.tag: INSDC center name
attributes.value: Qinghai Provincial People's Hospital
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16311:sh_HNRNPA2B1_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: KYSE150
attributes.tag: cell type
attributes.value: epitheloid cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: esophageal squamous carcinoma
attributes.tag: genetic modification
attributes.value: Lentivirus-mediated knockdown of HNRNPA2B1 repeats
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sample name
attributes.value: E-MTAB-16311:sh_HNRNPA2B1_1
attributes.tag: scientific_name
attributes.value: Homo sapiens