title: Sample 4
organism: Mus musculus
description: Protocols: After 8 hours of visual stimulation on the last day of the experiment, all mice were sacrificed by rapid cervical dislocation. The eyes were dissected and immediately processed for the collection of neural retina samples. The eye was punctured with a 30 G needle in the pars plana; a small microdissecting scissors were inserted into this puncture; and the entire anterior segment of the eye was cut off and the lens was pushed out. The neural retina was gently pulled away from the posterior eye cup (containing retinal pigment epithelium, choroid and sclera) and placed into ice-cold homogenization buffer. Ten neural retinas from five mice were pooled as one sample in both stimulated and control groups and homogenized with a Teflon-glass tissue grinder. The homogenates were then immunoprecipitated with an antibody to pS6 Ser244/247 (#2215, Cell Signaling Technology, Danvers, MA, USA). Mice were fed ad libitum and kept on a 12-hour light/dark cycle. Visual stimulation was performed in optomotor drums for 14 days during the whole light phase. In the optomotor drums, mice were exposed to vertical black and white stripes rotating with a spatial frequency of 0.2 cycles per degree and alternating clockwise and counterclockwise every 20 s. RNA was extracted from pS6 immunoprecipitates using the RNeasy Micro Kit (Qiagen, Hilden, Germany) according to manufacturer's instructions. The sequencing libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara, Shiga, Japan) following the manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2026-04-03
attributes.tag: INSDC center name
attributes.value: Laboratory of Genomics and Bioinformatics Institute of Molecular Genetics of the Czech Academy of Sciences
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13998:Sample 4
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: 16-20
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: neural retina
attributes.tag: sample name
attributes.value: E-MTAB-13998:Sample 4
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: stimulus
attributes.value: visual stimulation
attributes.tag: strain
attributes.value: C57BL/6J