title: 2022_30
organism: Mus musculus
description: Protocols: Mice were euthanised via isoflurane inhalation and perfused with PBS to remove circulating immune cells. Tumors were dissected and sampled for (immuno)histological and genomic analyses. Remaining tissue was minced into a slurry and digested in RPMI-1640 medium (Gibco, #42401-018) containing 0,25 mg/ml of Liberase HD (Roche, #05401089001) for 30 min at 37°C. Enzymatic digestion was halted by adding 10% FBS in RPMI (Gibcon #10270106) and tumor cell suspensions were collected by centrifugation (1200 rpmi for 5 min)and mechanical dissociation and filtering (30µm MACS SmartStratiners, Miltenyi Biotec, #130-098-458)was performed to obtain single cell suspension  single-cell suspensions were labelled with DAPI (Invitrogen, #D1306) and subjected to FACS to recover viable cells. 20,000 of viable  were loaded into one channel of the Chromium system using the V3 and V3.1 single-cell reagent kits (10X Genomics Following capture and lysis, cDNAs were synthesized, then amplified by PCR for 12 cycles as per the manufacturer's protocol (10X Genomics).
attributes.tag: ENA first public
attributes.value: 2025-05-30
attributes.tag: INSDC center name
attributes.value: 1Mondor Institute for Biomedical Research (IMRB), INSERM, Créteil, France
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14222:2022_30
attributes.tag: age
attributes.value: 14 months
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: Tumor MPNST
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult Tumor MPNST
attributes.tag: disease
attributes.value: Neurofibromatosis type 1
attributes.tag: genotype
attributes.value: Prss56cre/+, R26mt/+, NF1flox/-
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sample name
attributes.value: E-MTAB-14222:2022_30
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6