organism: Capra hircus
description: The epithelial cell lines employed in this study were derived from the lateral skin tissue of 3 fetal Inner Mongolian cashmere goats, aged 55 d. These cell lines were isolated through the tissue explant attachment method and purified via enzymatic digestion during previous research conducted by our research group. They were subsequently cryopreserved in liquid nitrogen for future use. A total of 2×105 well-grown epithelial cells were seeded into 6-well plates and cultured in a 37°C, 5% CO2 incubator. Once the cells reached 80% confluence, the medium was replaced with an Exo-free medium, and the cells were further cultured for 48 hours. Following this, the cell supernatants were collected and subjected to a series of centrifugation steps: initially at 2000×g for 10 minutes at 4°C to remove debris, followed by a higher centrifugation speed at 10000×g for 30 minutes at 4°C to eliminate larger vesicles. The supernatant was then carefully transferred to a new tube and ultracentrifuged at 110000×g for 70 minutes at 4°C using an ultracentrifuge rotor. The supernatant was discarded, and the pellet was resuspended in precooled 1×PBS before being subjected to another round of ultracentrifugation under the same conditions. Finally, the supernatant was again discarded, and the Epi-Exos were resuspended in an appropriate volume of 1×PBS. The extracted Epi-Exos were promptly analyzed or stored at -80°C for future use.
attributes.tag: strain
attributes.value: Aerbasi
attributes.tag: breed
attributes.value: Inner Mongolia cashmere goat
attributes.tag: dev_stage
attributes.value: 55 days of embryonic period
attributes.tag: collection_date
attributes.value: 2018-12
attributes.tag: geo_loc_name
attributes.value: China:Hohhot
attributes.tag: sex
attributes.value: not determined
attributes.tag: tissue
attributes.value: lateral skin tissue
attributes.tag: cell_line
attributes.value: epithelial
attributes.tag: cell_type
attributes.value: The epithelial cell lines utilized in this study originated from the lateral skin tissue of 55-day-old fetal Inner Mongolian cashmere goats.
attributes.tag: sample_type
attributes.value: epithelial cell-derived exosomes
attributes.tag: BioSampleModel
attributes.value: Model organism or animal