title: 7dMG0_3_S52_R1_001
organism: Mus musculus
description: Osteoblasts isolated from the parietal bone of Mus musculus
attributes.tag: sample_name
attributes.value: Lot 3 MG0 day7 R1
attributes.tag: age
attributes.value: 2 days
attributes.tag: biomaterial_provider
attributes.value: Yamaguchi university Department of Pharmacology
attributes.tag: birth_date
attributes.value: 2022-05-09
attributes.tag: birth_location
attributes.value: Yamaguchi University Animal Facility
attributes.tag: breed
attributes.value: C57BL/6
attributes.tag: breeding_history
attributes.value: F1 generation from C57BL/6 parents
attributes.tag: breeding_method
attributes.value: Natural mating
attributes.tag: cell_subtype
attributes.value: primary osteoblast
attributes.tag: cell_type
attributes.value: Osteoblast
attributes.tag: collected_by
attributes.value: Tetsuya Seto
attributes.tag: collection_date
attributes.value: 2022-07-21
attributes.tag: cultivar
attributes.value: missing
attributes.tag: death_date
attributes.value: 2022-05-11
attributes.tag: dev_stage
attributes.value: neonate
attributes.tag: disease
attributes.value: None
attributes.tag: ecotype
attributes.value: not applicable
attributes.tag: genotype
attributes.value: wild-type
attributes.tag: geo_loc_name
attributes.value: Japan:Yamaguchi, Ube
attributes.tag: growth_protocol
attributes.value: Cells were cultured in alpha NEM with 10% FBS at 37C in 5% CO2.
attributes.tag: health_state
attributes.value: Healthy
attributes.tag: isolate
attributes.value: missing
attributes.tag: isolation_source
attributes.value: Calvarial bone
attributes.tag: phenotype
attributes.value: normal
attributes.tag: sample_type
attributes.value: tissue
attributes.tag: sex
attributes.value: missing
attributes.tag: store_cond
attributes.value: Stored at -80C
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: tissue
attributes.value: calvarial bone osteoblast
attributes.tag: treatment
attributes.value: Methylglyoxal was administered at concentrations of 0 uM or 300 uM for 7 days, every 6 hours