title: HIV-V-A004-HA004-0062
organism: Homo sapiens
description: healthy, HIV-1-uninfected
attributes.tag: ENA first public
attributes.value: 2024-11-16
attributes.tag: INSDC center name
attributes.value: WASHINGTON UNIVERSITY SCHOOL OF MEDICINE
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 23917
attributes.tag: broad-scale environmental context
attributes.value: unknown
attributes.tag: collection date
attributes.value: 2015-08-25
attributes.tag: common name
attributes.value: human
attributes.tag: environmental medium
attributes.value: stool
attributes.tag: geographic location (country and/or sea)
attributes.value: USA
attributes.tag: geographic location (latitude)
attributes.value: 38.7946
attributes.tag: geographic location (longitude)
attributes.value: -106.5348
attributes.tag: geographic location (region and locality)
attributes.value: unknown
attributes.tag: host age
attributes.value: 38
attributes.tag: host race
attributes.value: WHITE
attributes.tag: host sex
attributes.value: female
attributes.tag: host subject id
attributes.value: 62
attributes.tag: library construction method
attributes.value: 16S rDNA sequencing
attributes.tag: library reads sequenced
attributes.value: 35446
attributes.tag: local environmental context
attributes.value: unknown
attributes.tag: nucleic acid amplification
attributes.value: PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA, with the reverse PCR primer barcoded with a 12-base error-correcting Golay code to facilitate multiplexing and used to amplify the V4 region of the bacterial 16S gene Caporaso, J.G. et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample, Proc. Natl. Acad. Sci. U.S.A. 108 (supplement_1) 4516-4522, https://doi.org/10.1073/pnas.1000080107 (2011).
attributes.tag: nucleic acid extraction
attributes.value: Phenol:chloroform
attributes.tag: project name
attributes.value: APPROACH Microbiome
attributes.tag: sample name
attributes.value: 23917
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 200 mg stool
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sequencing method
attributes.value: MiSeq 2 _ 250 paired-end