title: Sample 7
organism: Mus musculus
description: Protocols: A clonal population of immortalised bone marrow derrived macrophages following CRISPR KO of Egr1 or CRISPR against no target (i.e. WT) were infected with Salmonella enterica sv. Typhimurium (strain SL1344) at a multiplicity of infection of 10 or mock-infected. Gentamicin was added to cultures at 15 min post infection to kill extracellular bacteria. RNA was extracted from cultured cells at the indicated time point by Trizol purification according to manufacturer's instructions Libraries were generated with the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD according to manufacturer's instructions
attributes.tag: ENA first public
attributes.value: 2025-08-30
attributes.tag: External Id
attributes.value: SAMEA114236722
attributes.tag: INSDC center name
attributes.value: Department of Pathology, University of Cambridge
attributes.tag: INSDC last update
attributes.value: 2023-08-04T10:26:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13212:Sample 7
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: immortalised bone marrow derived macrophage
attributes.tag: cell_type
attributes.value: macrophage
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: infect
attributes.value: mock
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-13212:Sample 7
attributes.tag: scientific_name
attributes.value: Mus musculus