title: 2022_37
organism: Mus musculus
description: Protocols: 20 nerves per mouse were dissected and HBSS was added (Sigma, ref: H6648-500ML) on ice. After mechanical dissociation, nerves were transfered in a mix of enzymes (0,15% Trypsin (Sigma #T9201), 0,04% Hyaluronidase (Bioconcept #LS005474), 0,3% Collagenase type 2 (Bioconcept #LS004174) and DNAse I (25 μg/mL)) in HBSS, during 2 hours in a water bath. After digestion, nerves were tritured with a 1000 pipette and a solution of 20% FBS in RPMI (Gibco, ref: 42401018) was added to stop digestion. Tubes were centrifuged and supernatant removed. Single viable cells were sorted (BD InfluxTM Cell Sorter) using a nuclear labelling DRAQ7TM Far-Red Fluorescent Live-Cell Impermeant DNA Dye (Abcam, ref: ab109202). Cells were collected in 20% FBS-RPMI solution. Cells were centrifuged, the supernatant removed, and myelin depletion was performed by using anti-myelin beads (Miltenyi Biotec, ref: 130-096-733) in a 0,5% of MACS solution (Miltenyi Biotec, ref: 5130-091-376). Cells were collected after the passage through the magnetic separator. Around 25,000 cells were loaded into one channel of the Chromium system using the V3.1 single cell reagent kit (10X Genomics) to generate single-cell GEMs Following capture and lysis, cDNA was synthesized, then amplified by PCR for 12 cycles as per the manufacturer's protocol (10X Genomics).
attributes.tag: ENA first public
attributes.value: 2025-08-30
attributes.tag: External Id
attributes.value: SAMEA114337028
attributes.tag: INSDC center name
attributes.value: INSERM U955
attributes.tag: INSDC last update
attributes.value: 2023-09-07T14:13:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13334:2022_37
attributes.tag: age
attributes.value: 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Prss56cre,R26mt
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth condition
attributes.value: animals kept alone
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: subcutaneous nerve
attributes.tag: sample name
attributes.value: E-MTAB-13334:2022_37
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6