title: E135_F1_S14
organism: Mus musculus
description: Protocols: Experimental F1 embryos were generated by crossing Xist+/∆ (B6) dams carrying Oct4-EGFP transgene, and CAST sires, producing F1 female (XX(Xist∆)) and males (XY) that were analyzed.   Following timed natural matings, F1 embryos were harvested daily at E10.5 to E16.5. Gonads were dissected and dissociated into single-cell suspension. EGFP fluorescence was used to isolate the fluorescently marked germ cells with FACS. Viable individual germ cells and somatic cells were sorted by FACS into 96-well plates containing 4µl lysis buffer. Please see 'nucleic acid library construction protocol'. Single-cell RNA-seq libraries were generated as described in Picelli et al., 2014, Nature Protocols. Full-length RNA-seq from single cells using SMART-seq2.
attributes.tag: ENA first public
attributes.value: 2025-04-29
attributes.tag: INSDC center name
attributes.value: King's college London
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13535_5:E135_F1_S14
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: germ cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: E13.5
attributes.tag: embryo_id
attributes.value: F1
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: gonad
attributes.tag: sample name
attributes.value: E-MTAB-13535_5:E135_F1_S14
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: castaneus x C57B6Nj