title: HW-72h-L3
organism: Epinephelus fuscoguttatus x Epinephelus lanceolatus
description: Protocols: Before the start of the experiment, 600 healthy hybrid groupers (with an average body weight of 42.3 ± 2.8 g) were acclimatized in a recirculating aquaculture system (water temperature: 28–30°C; pH: 7.8 ± 0.2) and fasted for 24 hours. Subsequently, the fish were randomly assigned to two treatment groups: the control group (con), which received no treatment; and the Vibrio harveyi-infected group (HW), which was administered Vibrio harveyi via oral gavage. Total RNA was extracted from the liver of hybrid grouper by using the TRlzol Reagent and following the manufacturer's instructions (Life Technologies, California, USA). cDNA libraries were constructed as per the instruction manual of NEBNext Ultra RNA Library Prep Kit.
attributes.tag: ENA first public
attributes.value: 2025-08-30
attributes.tag: INSDC center name
attributes.value: Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, China.
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15492:HW-72h-L3
attributes.tag: age
attributes.value: 0.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: hybrid giant grouper
attributes.tag: developmental stage
attributes.value: fine
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: same
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-15492:HW-72h-L3
attributes.tag: scientific_name
attributes.value: Epinephelus fuscoguttatus x Epinephelus lanceolatus
attributes.tag: sex
attributes.value: male
attributes.tag: stimulus
attributes.value: Vibrio harveyi
attributes.tag: strain
attributes.value: Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂