title: two_hit_LPS20
organism: Mus musculus
description: Protocols: Lysis of cells was performed on plate after removal of medium and 1x wash with PBS.  RNA RNA was extracted using the RNeasy Mini Kit automatically via QIAcube®Connect (Qiagen, Hamburg, Germany). 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by cDNA sequencing library preparation utilizing the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®.
attributes.tag: ENA first public
attributes.value: 2026-01-09
attributes.tag: INSDC center name
attributes.value: Biomedical Informatics and Systems Medicine Justus-Liebig-University Giessen
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16236:two_hit_LPS20
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: WI-38
attributes.tag: cell type
attributes.value: fibroblast
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-16236:two_hit_LPS20
attributes.tag: scientific_name
attributes.value: Mus musculus