title: Sample 9
organism: Ovis aries
description: Protocols: At the slaughter, immediately after the sacrifice, samples from the perirenal and tail adipose tissues were taken from each of the eight animals. Both tissues were kept for 24 hours at 4°C in an RNA-stabilization solution (Ambion RNAlater; Life Technologies). Following the removal of the RNA-stabilization solution, the samples were stored at -80°C until the extraction of RNA. These lambs were reared at the commercial flock of the Instituto Ganadero de Montaña (IGM; Grulleros, León, Castile and León, Spain). All were male lambs born in the same lambing season (January-February 2020). The lambs were reared under the same management and feeding conditions. After birth and receiving the colostrum from their dams, lambs were fed ad libitum with a milk replacer (Ovilac 60; Calfvet®). The animals were sacrificed once they reached a body weight between 10 and 12.5 kg in vivo weight, and a maximum age of 35 days. RNA extraction was carried out for the chosen samples using the miRNeasy Mini Kit from Qiagen® adjusted for use with adipose tissue. Using an Agilent 2100 Bioanalyzer (Agilent Technologies) to evaluate the RNA integrity value of the samples, values greater than 7.2 were obtained for every sample. Following the acquisition of RNA, the cDNA libraries were constructed using the UltraTM RNA Library Prep Kit (NEBNext®) by Novogene (Cambridge, United Kingdom). A minimum of 0.8 μg of RNA per sample was used to prepare a total of 16 stranded paired-end libraries.
attributes.tag: ENA first public
attributes.value: 2025-08-31
attributes.tag: INSDC center name
attributes.value: Departamento de Producción Animal
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15357:Sample 9
attributes.tag: age
attributes.value: 36
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: sheep
attributes.tag: developmental stage
attributes.value: Suckling Lamb
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: 13
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: tail adipose tissue
attributes.tag: sample name
attributes.value: E-MTAB-15357:Sample 9
attributes.tag: scientific_name
attributes.value: Ovis aries
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: Assaf