title: Sample 7
organism: Homo sapiens
description: Protocols: Frozen peripheral blood mononuclear cells (PBMCs) were thawed at 37°C and diluted five times 1:1 in RPMI containing 10% FBS, following the 10x Genomics protocol for fresh frozen human PBMCs. Dead cells were removed using a Dead Cell Removal Kit (Miltenyi Biotec), and cell clumps were filtered with 40 µm Flowmi® strainers (SP Industries). Cell counts were determined microscopically prior to single-cell capture. No separate RNA extraction was performed. Single-cell suspensions were directly loaded into the 10x Chromium™ Controller for gel bead-in-emulsion (GEM) generation and reverse transcription. Reverse transcription, cDNA amplification, and library construction were performed using Chromium™ Single Cell V(D)J Reagent Kits with V1 Chemistry (10x Genomics), following the manufacturer's protocol. Target recovery was 10,000 cells per sample without sample multiplexing.
attributes.tag: ENA first public
attributes.value: 2025-08-30
attributes.tag: INSDC center name
attributes.value: Department of Microbiology, Faculty of Science, Mahidol University
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15328:Sample 7
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mononuclear cell
attributes.tag: clinical information
attributes.value: Acute
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: dengue disease
attributes.tag: disease staging
attributes.value: Dengue Fever
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-15328:Sample 7
attributes.tag: scientific_name
attributes.value: Homo sapiens