title: Sample 8
organism: Homo sapiens
description: Protocols: After 48 hours of mock or infection, cells were washed, and trypsinized and resuspended in PBS and BSA to prevent clumping.  Cells were grown in complete DMEM or IMDM media supplemented with 10% FBS.  Cells were either mock infected or infected with virus at varying MOIs for 48 hours.  Cells were lysed, and RT-PCR was used to capture host transcriptome, viral genomes, and sgRNAs to identify perturbed cells. cDNA was size selected to generate a larger cDNA library (transcriptomic) and a smaller DNA library (viral and CRISPR) .  10X genomics 5' VDJ protocol was followed for the transcriptomic library, READ2 was placed onto cDNA in small viral and CRISPR library by PCR before re-incorporation into the remaining protocol.
attributes.tag: ENA first public
attributes.value: 2026-04-18
attributes.tag: INSDC center name
attributes.value: Stanford University
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15272:Sample 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HuH-7.5
attributes.tag: cell type
attributes.value: hepatocyte
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: Flavivirus Infections
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: infect
attributes.value: YFV infected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-15272:Sample 8
attributes.tag: scientific_name
attributes.value: Homo sapiens