title: NC_T2_A2
organism: Saccharomyces cerevisiae
description: Protocols: The yeast culture was treated and then centrifuged. Yeast cells (BY4741) were grown at 30°C at 200 r.p.m. in YPD medium. The cells were grown in the presence or absence of lanosterol (LST) at a final concentration of 1 μM. Post-metabolite loading, the cells were either treated or untreated with the α-factor at a final concentration of 30 μM for 2 hours. RNA isolation was done using TRIzol Reagent. The sample was homogenized with TRIzol Reagent, followed by addition of chloroform. The solution was allowed to separate into different layers, and the upper aqueous layer was transferred to isopropanol, in the presence of glycogen for precipitation. The precipitated RNA was washed with ethanol, and resuspended in nuclease-free water. DNAse treatment was done to remove DNA contamination. The pure RNA was again precipitated with ethanol, washed and resuspended in nuclease free water for downstream processing. the nucleic acid library was successfully prepared
attributes.tag: ENA first public
attributes.value: 2025-01-30
attributes.tag: External Id
attributes.value: SAMEA113542861
attributes.tag: INSDC center name
attributes.value: Associate Professor The Chemosensory Lab Department of Computational Biology Indraprastha Institute of Information Technology-Delhi (IIIT-Delhi)
attributes.tag: INSDC last update
attributes.value: 2023-05-23T15:44:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12992:NC_T2_A2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: YPD medium at 30 degree Celsius
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-12992:NC_T2_A2
attributes.tag: scientific_name
attributes.value: Saccharomyces cerevisiae
attributes.tag: stimulus
attributes.value: 30 μM alpha-factor
attributes.tag: strain
attributes.value: BY4741