title: female gWAT day 0, 4h
organism: Mus musculus
description: Protocols: Mouse inguinal and gonadal white adipose tissues where excised from 6-10 weeks old C57BL/6NJ (B6N) mice fed ad libitum with SDS maintenance chow (RM3, 3.6 kcal/g).Primary preadipocytes were isolated from the WAT depots by by collagenase digestion and centrifugation to separate floating adipocytes from the SVF pellet. The SVF was resuspended in DMEM GlutaMax culture medium supplemented with 10% FBS and 1% PEST. The cell suspension was filtered through a 40 um mesh and pooled cells from 6-12 mice were seeded on a 10 cm dish at a density of 100.000 cells/mL.Cells were grown to confluence, and induced to differentiate 2 days post confluency by addition of 0.5 mM IBMX, 1 uM Dex, 5 ug/mL human Insulin to the growth media. On days -1, 0, 0,4h, 1, 2, 7 and mature stage, cells lysed directly in the cell culture plate and nuclei released. Lysis buffer was gently pipetted up and down to wash nuclei off the well and transferred into a chilled 1.5ml tube to create crude nuclei. Nuclei were spun down at 600 x g for 10 min at 4℃, nuclei pellets were then re-suspended in 40 µl Tagmentation DNA (TD) Buffer. Transposition reaction was performed as previously described (Buenrostro et al. 2015). All tagmented DNA was PCR amplified for 11-13 cycles. Quality was assessed using a DNA1000 Chip (Applied Biosystems) and run on a Bioanalyzer (Applied Biosystems). The profiles showed that all libraries had a mean fragment size of ~190 bp and characteristic nucleosome patterning, indicating good quality of the libraries.
attributes.tag: ENA first public
attributes.value: 2025-05-12
attributes.tag: External Id
attributes.value: SAMEA114630730
attributes.tag: INSDC center name
attributes.value: UiB
attributes.tag: INSDC last update
attributes.value: 2023-11-16T19:25:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13540:female gWAT day 0, 4h
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: preadipocyte
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: gonadal white adipose tissue
attributes.tag: sample name
attributes.value: E-MTAB-13540:female gWAT day 0, 4h
attributes.tag: sampling time point
attributes.value: 4 hours of adipogenic differentiation
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female