title: microcolony_11
organism: Escherichia coli
description: Protocols: Microcolonies were selected from an anaerobic LB plate are kept at -80C. Bacteria were regrown in LB at 37C with shaking for 5h. DNA was extracted from 1 mL of bacteria using the DNeasy Blood & Tissue Kit (Qiagen). Library preparation and sequencing was carried out by the Genomics Applications Laboratory, Core Research Facility, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel. DNA libraries for sequencing were prepared using the kit (ILMN DNA LP (M) Tagmentation (24 Samples, IPB) 20060060) (Illumina, Inc., USA) according to manufacturing instructions.
attributes.tag: ENA first public
attributes.value: 2025-05-31
attributes.tag: External Id
attributes.value: SAMEA114497837
attributes.tag: INSDC center name
attributes.value: The Racah Institute of Physics The Hebrew University of Jerusalem Jerusalem 9112001, Israel
attributes.tag: INSDC last update
attributes.value: 2023-10-13T16:00:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13428:microcolony_11
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: genetic modification
attributes.value: none
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: microcolony
attributes.value: microcolony_11
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-13428:microcolony_11
attributes.tag: scientific_name
attributes.value: Escherichia coli
attributes.tag: strain
attributes.value: O127:H6 strain E2348/69