title: hNSC_WT3
organism: Homo sapiens
description: Protocols: Total RNA was extracted from fresh pellets of iPSC or NCS using the RNeasy Mini Kit (Qiagen, 74104). The H9 human embryonic stem cell (hESC) derived neural stem cells (hNSCs) were purchased from Invitrogen (N7800-100). no dissection required The hNSCs were cultured on Geltrex-coated dishes (Geltrex LDEV-free reduced growth factor membrane matrix, Thermo Fisher Scientific, A1413202) in KnockOut DMEM/F12 (Invitrogen, 12660012) supplemented with 2 mM L-Glutamine (Thermo Fisher Scientific, 25030024), 20 ng/ml EGF (Peprotech, 315-09), 20 ng/ml FGF (Peprotech, 100-18B) and 2% of StemPro Neural Supplement (Thermo Fisher Scientific, A1050801). The media was refreshed every other day and cells were routinely passaged with Accutase (Sigma-Aldrich, A6964).  no treatment protocol was required nor performed on any cell line. no conversion protocol was required nor performed on any cell line. Total RNA was extracted from fresh pellets of iPSC or NCS using the RNeasy Mini Kit (Qiagen, 74104). Purified RNA was quantified using a NanoDrop spectrophotometer and RNA quality was checked with an Agilent 2100 Bioanalyzer using the RNA nano kit (Agilent, 5067-1512). RNA was isolated using the GenElute™ Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, RTN350-1KT) Library preparation for RNA sequencing was performed according to TruSeq Total RNA sample preparation protocol (Illumina, RS-122-2202), starting from 250 ng - 1 μg of total RNA. cDNA library quality was assessed on Agilent 2100 Bioanalyzer, using the high sensitivity DNA kit (Agilent 5067-4626) Total RNA was prepared using the Illumina TruSEq Stranded mRNA library Prep kit
attributes.tag: disease
attributes.value: none
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: collection date
attributes.value: not collected
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: cell type
attributes.value: neural stem cell
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: embryonic stem cell
attributes.tag: developmental stage
attributes.value: embryonic stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: cell line
attributes.value: WT
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected