title: 200324_EXTNEG
organism: unidentified
description: All_PCR_reaction_was_pooled. DNA extraction negative control (all dna extraction buffers without sample)
attributes.tag: broad-scale environmental context
attributes.value: not applicable
attributes.tag: collection date
attributes.value: not applicable
attributes.tag: target gene
attributes.value: 16S
attributes.tag: geographic location (longitude)
attributes.value: not applicable
attributes.tag: license information
attributes.value: not applicable
attributes.tag: nucleic acid extraction
attributes.value: Maxwell RSC PureFood GMO and Authentication Kit AS1600
attributes.tag: nucleic acid amplification
attributes.value: https://dx.doi.org/10.17504/protocols.io.kqdg3dzzl25z/v2
attributes.tag: geographic location (country and/or sea)
attributes.value: not applicable
attributes.tag: adapters
attributes.value: fwd: AATGATACGGCGACCACCGAGATCTACACGCTTATGGTAATTGT, rev: CAAGCAGAAGACGGCATACGAGATAGTCAGCCAGCC
attributes.tag: sample coordinator ORCID
attributes.value: not applicable
attributes.tag: organism
attributes.value: unidentified
attributes.tag: environmental medium
attributes.value: not applicable
attributes.tag: geographic location (latitude)
attributes.value: not applicable
attributes.tag: scientific_name
attributes.value: unidentified
attributes.tag: target subfragment
attributes.value: V4
attributes.tag: geographic location (region and locality)
attributes.value: not applicable
attributes.tag: sample storage duration
attributes.value: not applicable
attributes.tag: pcr primers
attributes.value: fwd: GTGYCAGCMGCCGCGGTAA, rev: GGACTACNVGGGTWTCTAAT
attributes.tag: pcr conditions
attributes.value: initial denaturation:94degC_3min; denaturation:94degC_45s; annealing:50degC_60s; extension:72degC_90s; final extension:72degC_10min;
attributes.tag: sample coordinator
attributes.value: not applicable
attributes.tag: sample coordinator affiliation
attributes.value: not applicable
attributes.tag: local environmental context
attributes.value: not applicable
attributes.tag: project name
attributes.value: Microbiota Vault
attributes.tag: multiplex identifiers
attributes.value: AATGATACGGCGACCACCGAGATCTACACGCT
attributes.tag: sample biobank
attributes.value: not applicable
attributes.tag: sequencing_run
attributes.value: run_409