title: E086 siPRAJA input
organism: Homo sapiens
description: Protocols: cells were collected using standard protocols  standard protocol standard protocols for cell lines cells where treated with compound for certain number of hours.  standard protocol Total RNA extraction was performed using the TRI reagent (Merck T9424) following the manufacturer's instruction Total RNA concentrations were determined by using NanoDrop spectrophotometer (Thermo Fisher) and their quality were assessed with the TapeStation 4150 (Agilent Technologies). Briefly, 100 ng of RNA of each sample were subjected to mRNAs capture by using polyT-coated beads followed by the indexed library preparation with the SureSelect XT HS2 mRNA Library Preparation System (Agilent Technologies), according to the manufacturer's instructions
attributes.tag: ENA first public
attributes.value: 2025-05-31
attributes.tag: INSDC center name
attributes.value: Laboratory of Molecular Medicine and Genomics - University of Salerno
attributes.tag: INSDC status
attributes.value: public
attributes.tag: RNA interference
attributes.value: siPRAJA
attributes.tag: Submitter Id
attributes.value: E-MTAB-14326:E086 siPRAJA input
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: glioblastoma
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sample name
attributes.value: E-MTAB-14326:E086 siPRAJA input
attributes.tag: scientific_name
attributes.value: Homo sapiens