title: K1L
organism: Mus musculus
description: Protocols: Freshly-harvested liver tissues from mice (n = 3 male mice per group) were snap frozen in liquid nitrogen before storage in a low-temperature freezer at -80°C. Total RNA was isolated from the liver tissue samples using the RNeasy Mini Kit (Qiagen) After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo(dT) beads. For prokaryotic organisms or eukaryotic organisms' long-non-coding libraries, rRNA is removed using the Ribo-Zero kit that leaves the mRNA. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
attributes.tag: ENA first public
attributes.value: 2025-05-31
attributes.tag: External Id
attributes.value: SAMEA113577864
attributes.tag: INSDC center name
attributes.value: University of Edinburgh Centre for Inflammation Research
attributes.tag: INSDC last update
attributes.value: 2023-06-07T15:58:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13049:K1L
attributes.tag: age
attributes.value: 16
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not provided
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: KmoNull
attributes.tag: geographic location (country and/or sea)
attributes.value: not provided
attributes.tag: individual
attributes.value: 1
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-13049:K1L
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL6J