title: Sample 7
organism: Danio rerio
description: Protocols: at the end of exposure, 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep-24 homogeniser (MP Biomedicals, Irvine, USA) at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to tebuconazole for 96 hours according to OECD test guidelines No. 236 at 27 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub-lethal concentrations of Tebuconazole (CAS 107534-96-3) in addition to untreated control groups. total RNA and Protein were extracted from the tissue lysate using using the NucleoSpin® RNA/Protein kit (Macherey & Nagel, Düren, Germany). according to the manufacturer's protocol. Nanodrop 2000 spectrophotometer (Thermo Scientific) was used to measure and normalize the obtained RNA concentrations. The quality of the extracted RNA samples was checked using RNA 6000 Nano kit in 2100 Bioanalyzer System (Agilent, Santa Clara, USA). Samples were stored at -80°C.  RNA libraries were prepared by the Eurofins Genomics Germany GmbH from RNA extracts with RNA integrity number (RIN) values > 8.5 using poly(A) RNA purification followed by fragmentation. Using reverse transcription, cDNA libraries was produced from mRNA fragments and ligated to the adapters.
attributes.tag: ENA first public
attributes.value: 2025-04-30
attributes.tag: INSDC center name
attributes.value: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME)
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14535:Sample 7
attributes.tag: age
attributes.value: 96
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: zebrafish
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-14535:Sample 7
attributes.tag: scientific_name
attributes.value: Danio rerio
attributes.tag: strain
attributes.value: AB
attributes.tag: tank
attributes.value: T9