title: Gulf of Mexico seawater sample GU190710-CTD22-245
organism: seawater metagenome
attributes.tag: collection_date
attributes.value: 2019-07-10
attributes.tag: depth
attributes.value: 245
attributes.tag: env_broad_scale
attributes.value: marine biome [ENVO:00000447]
attributes.tag: env_local_scale
attributes.value: marine mesopelagic zone [ENVO:00000213]
attributes.tag: env_medium
attributes.value: sea water [ENVO:00002149]
attributes.tag: geo_loc_name
attributes.value: USA: Gulf of Mexico
attributes.tag: lat_lon
attributes.value: 28.390 N 85.540 W
attributes.tag: expedition_id
attributes.value: GU19-01
attributes.tag: ctd_bottle_number
attributes.value: CTD22
attributes.tag: depth_category
attributes.value: Bottom
attributes.tag: samp_category
attributes.value: sample
attributes.tag: habitat_natural_artificial_0_1
attributes.value: 0
attributes.tag: chlorophyll
attributes.value: 0.0598
attributes.tag: collection_method
attributes.value: CTD rosette
attributes.tag: density
attributes.value: 1027.8999
attributes.tag: diss_oxygen
attributes.value: 3.8858 mg/L
attributes.tag: fluor
attributes.value: 92.4053
attributes.tag: neg_cont_type
attributes.value: not applicable
attributes.tag: pos_cont_type
attributes.value: not applicable
attributes.tag: salinity
attributes.value: 35.8482
attributes.tag: samp_collect_device
attributes.value: Niskin bottle
attributes.tag: samp_mat_process
attributes.value: Samples were vacuum-filtered through a MilliporeSigma 47 mm diameter mixed cellulose ester (MCE) filter with a 0.45 µm pore size in a disposable 250 mL filter cup (MilliporeSigma EZ-Fit Filtration Unit) using an EZ-Fit 1-place manifold and EZ-Stream vacuum pump (MilliporeSigma).
attributes.tag: filter_passive_active_0_1
attributes.value: 1
attributes.tag: filter_diameter
attributes.value: 47
attributes.tag: filter_material
attributes.value: cellulose ester
attributes.tag: filter_name
attributes.value: MilliporeSigma
attributes.tag: prepped_samp_store_temp
attributes.value: ambient
attributes.tag: prepped_samp_store_sol
attributes.value: longmire
attributes.tag: prepped_samp_store_dur
attributes.value: P4M
attributes.tag: samp_size
attributes.value: 2 L
attributes.tag: samp_store_dur
attributes.value: P19M/35M
attributes.tag: samp_store_sol
attributes.value: none
attributes.tag: samp_store_loc
attributes.value: In cooler with ice
attributes.tag: samp_store_temp
attributes.value: 4
attributes.tag: samp_vol_we_dna_ext
attributes.value: 2 L
attributes.tag: nucl_acid_ext_lysis
attributes.value: physical | thermal
attributes.tag: nucl_acid_ext_sep
attributes.value: phenol chloroform
attributes.tag: nucl_acid_ext
attributes.value: https://doi.org/10.1002/edn3.70074
attributes.tag: nucl_acid_ext_kit
attributes.value: not applicable
attributes.tag: dna_cleanup_0_1
attributes.value: 1
attributes.tag: dna_cleanup_method
attributes.value: Zymo OneStep PCR Inhibitor Removal kit
attributes.tag: size_frac
attributes.value: 0.45
attributes.tag: size_frac_low
attributes.value: not applicable
attributes.tag: source_material_id
attributes.value: GU190710-CTD22-245
attributes.tag: temp
attributes.value: 14.1758
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.water