title: WT_F1_pollen2
organism: Arabidopsis thaliana
description: Protocols: To purify pollen grains from F1 hybrid plants and extract their genomic DNA, whole inflorescences of hybrid plants were collected and transferred to ice-cold 10% (w/v) sucrose in a 50-ml tubes. Flowers were homogenized with a blender (Tefal, BL3051KR), filtered through 80-μm nylon mesh, and centrifuged at 350g for 10 min at 4 °C. The supernatant was discarded and the pellet was resuspended in 10% (w/v) sucrose, filtered through a 40-μm cell strainer, and centrifuged at 100g for 10 min at 4°C. The purified pellet was frozen in liquid nitrogen. long day conditions (16 hr light), 20 degrees Celcius DNA was extracted using the CTAB method. Nanobind disk (Nanobind Plant Nuclei Kit RT, PacBIO, 102-302-000) was used to collect high molecular weight genomic DNA. Ligation Sequencing Kit V14 (Nanopore, SQK-LSK114)
attributes.tag: ENA first public
attributes.value: 2025-05-16
attributes.tag: INSDC center name
attributes.value: POSTECH
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15083:WT_F1_pollen2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: thale cress
attributes.tag: cultivar
attributes.value: Col/Ler F1
attributes.tag: developmental stage
attributes.value: plant structure development stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: pollen
attributes.tag: sample name
attributes.value: E-MTAB-15083:WT_F1_pollen2
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana