title: 15272
organism: Mus musculus
description: Protocols: Cells were seeded at a density of 10.000 cells in 6 cm dishes and after attachment, were cultured for 24 hours. Culture in standard medium +/- 60 µM 10058-F4 (Myc Inhibitor) Total RNA of cancer cell lines was isolated using Maxwell® 16 LEV simplyRNA Tissue Kit (AS1280, Promega) and Maxwell® 16 Instrument (Promega) as per manufacturer's instructions Library preparation for bulk 3'-sequencing of poly(A)-RNA was done as described previously (Parekh et al., 2016) barcoded cDNA of each sample was generated with a Maxima RT polymerase (Thermo Fisher) using oligo-dT primer containing barcodes, unique molecular identifiers (UMIs) and an adapter. 5' ends of the cDNAs were extended by a template switch oligo (TSO) and after pooling of all samples full-length cDNA was amplified with primers binding to the TSO-site and the adapter. cDNA was tagmented with the Nextera XT kit (Illumina) and 3'-end-fragments finally amplified using primers with Illumina P5 and P7 overhangs.
attributes.tag: ENA first public
attributes.value: 2025-12-23
attributes.tag: INSDC center name
attributes.value: Klinik und Poliklinik für Innere Medizin II TUM Klinikum rechts der Isar Universität München
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16320:15272
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: cancer cell line
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: pancreatic cancer
attributes.tag: genotype
attributes.value: KrasG12D
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: pancreas
attributes.tag: sample name
attributes.value: E-MTAB-16320:15272
attributes.tag: scientific_name
attributes.value: Mus musculus