title: H001AS8_A23
organism: Homo sapiens
description: Protocols: hPSC tranfected in pool with iPS-2seq plasmids in AAVS1 locus (multiple perturbations in pool). shRNA expression has been induced throughout differentiation with Tetracycline. hPSC derived cardiomyocytes were cultured for 23 days with tetracycline. Then Harvested at single cell suspension and processed for nuclear extraction. Nuclear extraction by IGEPAL 0.3% ipotonic buffer. PFA fixation. RNAse inhibitors have been used during all steps to avoid RNA degradation. 2-level indexing sci-RNAseq protocol for the gene expression library and the barcode library. shRNA barcodes have been enriched during RT and PCR indexing by a nested PCR binding the TET repressor.
attributes.tag: ENA first public
attributes.value: 2025-04-30
attributes.tag: INSDC center name
attributes.value: UNITO
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14102:H001AS8_A23
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: WTC11
attributes.tag: cell_type
attributes.value: hPSC derived cardiomyocytes
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: DD23
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: KD screen in pool (multiple)
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: hPSC derived cardiomyocytes
attributes.tag: sample name
attributes.value: E-MTAB-14102:H001AS8_A23
attributes.tag: scientific_name
attributes.value: Homo sapiens