title: Sample 17
organism: Homo sapiens
description: Protocols: Human peripheral blood was collected from healthy volunteers with written informed consent. This study was conducted in accordance with the Declaration of Helsinki and all protocols were approved by the Ethics Committee of the University Hospital Jena (permit number: 3639-12/12). After 8 hours of infection, human RNA was stabilized in PAXgene Blood RNA tubes (PreAnalytiX) and extracted using the PAXgene Blood RNA Isolation Kit (PreAnalytiX) according to the manufacturer's instructions. RNA concentration was measured with a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific), and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies).   Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.
attributes.tag: strain
attributes.value: ECHR/FKSmut (SN-2021-0056)
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: collection date
attributes.value: not collected
attributes.tag: stimulus
attributes.value: C. albicans
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: cell type
attributes.value: whole blood
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: blood
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected