title: Sample 8
organism: Homo sapiens
description: Protocols: Samples were fresh frozen in TRIzol Grown in a 37 °C humidified incubator in RPMI1640 media supplemented with 10% FBS. All media were further supplemented with 2 mM L-glutamine and 50 μg/ml penicillin/streptomycin. TTFields were applied to cells using the inovitroTM system as previously described (Kirson, E.D., et al., Disruption of cancer cell replication by alternating electric fields. Cancer Res, 2004. 64(9): p. 3288-95.) With frequency of 150 kHz and intensity of 1.7 V/cm RMS for 24 and 48 hours.  RNA was purified with TRI reagent as previously described [ Rio, D.C., et al., Purification of RNA Using TRIzol (TRI Reagent). Cold Spring Harbor Protocols, 2010. 2010(6): p. pdb.prot5439. ]   For cDNA libraries preparation, the polyA fraction (mRNA) was purified, followed by fragmentation and generation of double-stranded cDNA. Agencourt AMPure XP beads (Beckman Coulter) were used for cleanup, followed by end repair, UMIs addition, adapter ligation and PCR amplification steps.
attributes.tag: ENA first public
attributes.value: 2025-05-31
attributes.tag: INSDC center name
attributes.value: Novocure
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15132:Sample 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: AsPC-1
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: pancreatic ductal adenocarcinoma
attributes.tag: genotype
attributes.value: AsPC1
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: pancreas
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-15132:Sample 8
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: stimulus
attributes.value: Control