title: Exp2_HeLa_siCont_3
organism: Homo sapiens
description: Protocols: HeLa cells were grown in MEM medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. siRNA transfections were performed using DharmaFECT 1 Transfection Reagent (Horizon Discovery Ltd.) according to the manufacturer's instructions. RNA was extracted usiung EXTRAzol® Lysis Reagent (BLIRT) according to the manufacturer's protocol. Libraries were prepared using TrueSeq™ V2 RNA-Seq Library Prep Kit (Illumina®) after verifying the RNA integrity on an agarose gel. Library quality was assessed using an Agilent Bioanalyzer 2100 (High Sensitivity DNA Kit).
attributes.tag: ENA first public
attributes.value: 2025-03-09
attributes.tag: INSDC center name
attributes.value: Deptartment of Gynecology and Obstetrics University Medical Center Göttingen
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13917:Exp2_HeLa_siCont_3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: HeLa
attributes.tag: cell_type
attributes.value: cervical cancer
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: cervical cancer
attributes.tag: genotype
attributes.value: siControl (NT5)
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: uterine cervix
attributes.tag: sample name
attributes.value: E-MTAB-13917:Exp2_HeLa_siCont_3
attributes.tag: scientific_name
attributes.value: Homo sapiens