title: Pool sample of integron amplicon sequences
organism: metagenome
description: PacBio HiFi long-read sequencing was performed on a pool sample of integron amplicons obtained with the primers: intI1-R [GCGAACGARTGBCGVAGVGTGTG] +3CS[AAGCAGACTTGACCTGA] and intI3F [AGTGGGTGGCGAATGAGTG] + class3R [CGTCAAACGGGTAAGCAGT]. Twelve monthly samples were amplified with each primer and then pooled. Forward primers already had unique barcodes associated. Only one library preparation was performed (the barcodes inserted in library prep are: 5 end: CGCATGACACGTGTGTT; 3 end: AACACACGTGTCATGCG). The sample specific barcodes (are only present at the 5'end) are as follows: January_int1: ATCACG, February_int1: CGATGT, March_int1: TTAGGC, April_int1: TGACCA, May_int1: ACAGTG, June_int1: GCCAAT, July_int1: CAGATC, August_int1: ACTTGA, September_int1: GATCAG, October_int1: TAGCTT, November_int1: GGCTAC, December_int1: CTTGTA, January_int3: AGTCAA, February_int3: AGTTCC, March_int3: ATGTCA, April_int3: CCGTCC, May_int3: GTAGAG, June_int3: GTCCGC, July_int3: ACTGAT, August_int3: ATGAGC, September_int3: ATTCCT, October_int3: CAAAAG, November_int3: CAACTA, December_int3: CACCGG
attributes.tag: isolation_source
attributes.value: treated wastewater
attributes.tag: collection_date
attributes.value: 2020
attributes.tag: geo_loc_name
attributes.value: Portugal
attributes.tag: lat_lon
attributes.value: not provided
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental