title: LP08_SUPE: Superficial soil sample (SUP) collected from approximately one square meter of leaf litter and humus, and processed using flotation and Berlese extraction.
organism: soil metagenome
description: Bulk mesofauna samples were subjected to DNA extraction using the DNeasy Blood and Tissue Kit (Qiagen) on a KingFisher Flex robotic platform (Thermo Scientific). Samples were homogenized prior to extraction, and DNA extracts were quantified using a NanoDrop 1000 spectrophotometer. A 418 bp fragment from the 3' end of the mitochondrial COI barcode region (bc3') was amplified using primers Ill-B-F and Fol-degen-R with Illumina adapter tails. Three independent PCR reactions were performed per sample and pooled. Amplicons were purified using AMPure XP magnetic beads and used as template for a limited-cycle PCR to incorporate dual-index barcodes and Illumina sequencing adapters. Libraries were generated for high-throughput sequencing on Illumina platforms.
attributes.tag: isolate
attributes.value: Bulk of mesofauna specimens LP08_SUPE
attributes.tag: breed
attributes.value: not applicable
attributes.tag: isolation_source
attributes.value: soil mesofauna community samples
attributes.tag: collection_date
attributes.value: 2016/2020
attributes.tag: geo_loc_name
attributes.value: Spain: Canary Islands
attributes.tag: tissue
attributes.value: full body
attributes.tag: biomaterial_provider
attributes.value: Institute of Natural Products and Agrobiology (IPNA-CSIC)
attributes.tag: collected_by
attributes.value: Island Ecology and Evolution Group (GEEI)
attributes.tag: env_broad_scale
attributes.value: Macaronesian Laurel Forest
attributes.tag: lat_lon
attributes.value: 28.803862 N 17.820526 W
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental