title: DNA from Saccharina latissima strains SLPER139, F1.27.2 and 189 F2 progeny
organism: Saccharina latissima
description: DNA samples extracted for ddRAD-seq analysis from 17 mm discs excised from Saccharina latissima strains SLPER139, F1.27.2 and 189 F2 progeny produced by selfing F1.27.2.
attributes.tag: ENA first public
attributes.value: 2025-04-30
attributes.tag: Further Details
attributes.value: Two strains of Saccharina latissima collected at Perharidy (near Roscoff, north Finistere, Brittany, France, January 2016, strain SLPER139) and Saint Guenole (south Finistere, Brittany, France, October 2015, strain SLSTG055) were crossed to produce the F1 hybrid parent F1.27.2. A bulk cross using gametophytes derived from F1.27.2 then generated 189 F2 sporophytes. Strain F1.27.2 and 129 F2 hybrids were cultivated on an open water rope system in Morlaix Bay in 2017. The remaining 60 F2 hybrids were cultivated in the laboratory in natural seawater with half-strength Provasoli enrichment in aerated 10 L bottles at 13�C. After harvesting tissue discs were excised from the blades and stored dessicated in silica gel for DNA extraction. ddRAD-seq libraries were constructed with a size-selected range of fragments of 350-800 bp (before addition of the barcodes/adaptateurs/index).
attributes.tag: INSDC center name
attributes.value: SBR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Slat_PopF2_QTL
attributes.tag: amount or size of sample collected
attributes.value: 17 mm blade disc
attributes.tag: collected_by
attributes.value: Zofia Nehr
attributes.tag: collection date
attributes.value: 2017-10-01
attributes.tag: depth
attributes.value: 1
attributes.tag: geographic location (country and/or sea)
attributes.value: Atlantic Ocean
attributes.tag: geographic location (latitude)
attributes.value: 48.709483
attributes.tag: geographic location (longitude)
attributes.value: -3.953283
attributes.tag: growth condition
attributes.value: Either open water culture using seeded cotton strings attached to horizontal ropes attached to anchored bouys in Morlaix bay (strain F1.27.2 and 129 F2 hybrids) or laboratory cultivation in natural seawater with half-strength Provasoli enrichment in aerated 10 L bottles at 13�C (60 F2 hybrids).
attributes.tag: sample collection method
attributes.value: Excision of a 17 mm disc from the meristematic region of the blade.
attributes.tag: sample name
attributes.value: Slat_PopF2_QTL
attributes.tag: sample storage conditions
attributes.value: Silica gel
attributes.tag: sample storage duration
attributes.value: 6 years
attributes.tag: sample storage temperature
attributes.value: 20
attributes.tag: scientific_name
attributes.value: Saccharina latissima
attributes.tag: strain
attributes.value: SLPER139, F1.27.2 and 189 F2 progeny derived from F1.27.2