title: Sample 71
organism: Homo sapiens
description: Protocols: Human fetal tissue samples used for bulk-RNA seq were obtained with ethical approval and informed consent from the Human Developmental Biology Resource (http://www.hdbr.org).The age was estimated from foot length and knee-heel length in relation to standard growth data. Samples were karyotyped by quantitative PCR (chromosomes 13, 15, 16, 18, 21, 22 and X and Y) to determine the sex of the fetus. Samples were stored in -70C until use. Total RNA was extracted from human fetal tissue samples using the AllPrep DNA/RNA Mini Kit (Qiagen). cDNA libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche) on a Hamilton Starlet robotic platform (Hamilton) and quality was analyzed using a Tapestation 4200 platform (Agilent technologies).
attributes.tag: ENA first public
attributes.value: 2025-04-30
attributes.tag: External Id
attributes.value: SAMEA115057953
attributes.tag: INSDC center name
attributes.value: UCL-Great Ormond Street Institute of Child Health
attributes.tag: INSDC last update
attributes.value: 2023-12-22T14:40:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13673:Sample 71
attributes.tag: age
attributes.value: 15
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: 15wpc
attributes.tag: disease
attributes.value: control
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: fetal
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: karyotype
attributes.value: 46,XX
attributes.tag: organism part
attributes.value: muscle
attributes.tag: sample name
attributes.value: E-MTAB-13673:Sample 71
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: 46,XX