organism: Candida albicans
description: Biological replicate 3
attributes.tag: strain
attributes.value: SC5314
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: collection_date
attributes.value: 2024-04-15
attributes.tag: geo_loc_name
attributes.value: not applicable
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: genotype
attributes.value: tlo-null rep 3, LEU2+/-, MTLa/alpha
attributes.tag: mating_type
attributes.value: a/alpha
attributes.tag: passage_history
attributes.value: 2 passages total. -80C frozen stock was struck to solid YPD medium and incubated at 30C for 2 days. Cells from the densest part of the streak were then used to start an overnight culture in liquid YPD medium at 30C. Cells from the overnight culture were diluted 1:50 in fresh YPD and incubated at 30C for 2 hrs. RNA was then extracted and treated with DNaseI prior to mRNA isolation-based cDNA library preparation.
attributes.tag: temp
attributes.value: 30C
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental