title: SW-CL-YO-05
organism: soil metagenome
attributes.tag: ENA first public
attributes.value: 2025-09-11
attributes.tag: INSDC center name
attributes.value: Scotland's Rural College - Aberdeen Campus
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: ms00696
attributes.tag: adapters
attributes.value: Forward overhang: 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG; Reverse overhang: 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG
attributes.tag: broad-scale environmental context
attributes.value: environmental system determined by an organism
attributes.tag: chimera check software
attributes.value: N/A
attributes.tag: climate environment
attributes.value: controlled glasshouse
attributes.tag: collection date
attributes.value: 2021-09-06
attributes.tag: environmental medium
attributes.value: rhizosphere environment
attributes.tag: experimental factor
attributes.value: Spring Wheat
attributes.tag: geographic location (country and/or sea)
attributes.value: United Kingdom
attributes.tag: geographic location (latitude)
attributes.value: 54.2059327
attributes.tag: geographic location (longitude)
attributes.value: -1.0566978
attributes.tag: host age
attributes.value: 3
attributes.tag: host common name
attributes.value: Spring Wheat
attributes.tag: host life stage
attributes.value: flowering
attributes.tag: host subspecific genetic lineage
attributes.value: Mulika
attributes.tag: host taxid
attributes.value: 4565
attributes.tag: local environmental context
attributes.value: plant-associated environment
attributes.tag: multiplex identifiers
attributes.value: N/A
attributes.tag: negative control type
attributes.value: plant-associated environment
attributes.tag: nucleic acid amplification
attributes.value: https://www.novogene.com/eu-en/resources/blog/a-beginners-guide-to-16s-18s-its-amplicon-metagenomic-sequencing/?_gl=1*zg303z*_up*MQ..*_gs*MQ..&gclid=CjwKCAjw24vBBhABEiwANFG7y_L5jjZFt1XnOwrf0qRG07FrSPv8bvD-aqDeyMYkG-ytGAiddyaqpBoCgrEQAvD_BwE&gbraid=0AAAAApRAw_nj7fjNvzzuoPTcLafmAJU-A
attributes.tag: nucleic acid extraction
attributes.value: Boil (15 min.) and freeze cells in Tris 40mM, EDTA 0.4 mM, 0.4% Triton-X
attributes.tag: pcr conditions
attributes.value: 95 C for 3 minutes; 25 cycles of 95 C for 30 seconds, 55 C for 30 seconds, 72 C for 30 seconds; 72 C for 5 minutes; Hold at 4 C
attributes.tag: pcr primers
attributes.value: Forward: 5' GTGCCAGCMGCCGCGGTAA; Reverse: 5' CCGTCAATTCCTTTGAGTTT
attributes.tag: plant body site
attributes.value: root
attributes.tag: plant product
attributes.value: N/A
attributes.tag: positive control type
attributes.value: rhizosphere environment
attributes.tag: project name
attributes.value: UK Crop Microbiome Cryobank
attributes.tag: reference for biomaterial
attributes.value: Rothamsted Research Station
attributes.tag: relevant electronic resources
attributes.value: agmicrobiomebase.org
attributes.tag: sample name
attributes.value: ms00696
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 0.5
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sequence quality check
attributes.value: software
attributes.tag: sequencing method
attributes.value: NovaSeq
attributes.tag: target gene
attributes.value: 16S