organism: Homo sapiens
attributes.tag: isolate
attributes.value: missing
attributes.tag: age
attributes.value: 14
attributes.tag: biomaterial_provider
attributes.value: American Type Culture Collection (ATCC)
attributes.tag: collection_date
attributes.value: missing
attributes.tag: geo_loc_name
attributes.value: missing
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: peripheral blood
attributes.tag: cell_line
attributes.value: Jurkat
attributes.tag: cell_subtype
attributes.value: Clone E6-1
attributes.tag: cell_type
attributes.value: T lymphocyte
attributes.tag: disease
attributes.value: acute T cell leukemia
attributes.tag: vcn
attributes.value: Five integrated copies of CD19 CAR LVV in Jurkat genome. The integrated VCN was analyzed by ddPCR and NGS assays. Six LVV components from ddPCR assays were analyzed to determine the VCNs: LTR, Psi, RRE, CD19 CAR, eGFP. Since VCN5 cells were derived from VCN4, we detected five copies of LTR, Psi, RRE, CD19 CAR, and WPRE, and six copies of eGFP. VCN5 cells inherited insertions found in VCN4 and contained an insertion with expected sequence on chr6 (24,825,065, plus strand) and an insertion on chr3 (18,115,277, minus strand) with a single SNP. An additional insertion was found in chr1 (243,273,502 plus strand) with one SNP, however, at the end of 3 LTR sequence there was a 41 bp sequence of unknown origin. Moreover, a 2046 bp 3 portion of the proviral DNA, containing the eGFP gene, was found inserted in chr6 (15,601,063, minus strand). Additionally, VCN5 had an as expected insertion in chr5 (39,044,694, minus strand).
attributes.tag: BioSampleModel
attributes.value: Human