organism: Homo sapiens
attributes.tag: isolate
attributes.value: missing
attributes.tag: age
attributes.value: 14
attributes.tag: biomaterial_provider
attributes.value: American Type Culture Collection (ATCC)
attributes.tag: collection_date
attributes.value: missing
attributes.tag: geo_loc_name
attributes.value: missing
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: peripheral blood
attributes.tag: cell_line
attributes.value: Jurkat
attributes.tag: cell_subtype
attributes.value: Clone E6-1
attributes.tag: cell_type
attributes.value: T lymphocyte
attributes.tag: disease
attributes.value: acute T cell leukemia
attributes.tag: vcn
attributes.value: One integrated copy of CD19 CAR LVV in Jurkat genome. The integrated VCN was analyzed by ddPCR and NGS assays. Six LVV components from ddPCR assays were analyzed to determine the VCNs: LTR, Psi, RRE, CD19 CAR, eGFP. VCN1 contained one copy of each element per cell. With NGS, VCN1 showed one copy of proviral DNA inserted on chromosome (chr) 17 (78,730,345, minus strand). Further, there was a 724 bp deletion within the proviral DNA sequence in the EF1a promoter region.
attributes.tag: BioSampleModel
attributes.value: Human