title: SARS-CoV-2 from wastewater in Philadelphia
organism: wastewater metagenome
attributes.tag: isolation_source
attributes.value: wastewater
attributes.tag: geo_loc_name
attributes.value: USA: Pennsylvania, Philadelphia
attributes.tag: collected_by
attributes.value: Philadelphia Water Department
attributes.tag: sequenced_by
attributes.value: Philadelphia Public Health Laboratory
attributes.tag: purpose_of_ww_sampling
attributes.value: public health surveillance community-level
attributes.tag: purpose_of_ww_sequencing
attributes.value: SARS-CoV-2 surveillance
attributes.tag: collection_site_id
attributes.value: SW WPCP
attributes.tag: isolate
attributes.value: WW-2025-02-06-SouthWest
attributes.tag: specimen_processing_id
attributes.value: WW-2025-02-06-SouthWest-Rep4
attributes.tag: specimen_processing
attributes.value: biological replicate
attributes.tag: specimen_processing_details
attributes.value: Composite sample was aliquoted into 10 mL biological replicates before concentration, extraction, and sequencing (see specimen_processing_id)
attributes.tag: concentration_method
attributes.value: ceres nanotrap
attributes.tag: extraction_method
attributes.value: thermo magmax wastewater ultra nucleic acid isolation kit
attributes.tag: extraction_control
attributes.value: murine hepatitis virus
attributes.tag: project_name
attributes.value: CDC NWSS
attributes.tag: ww_surv_system_sample_id
attributes.value: WW-250205-250207-SW
attributes.tag: ww_sample_site
attributes.value: wastewater treatment plant
attributes.tag: ww_sample_matrix
attributes.value: raw wastewater
attributes.tag: ww_sample_type
attributes.value: composite
attributes.tag: ww_sample_duration
attributes.value: 24
attributes.tag: ww_surv_jurisdiction
attributes.value: PH
attributes.tag: ww_population
attributes.value: 517309
attributes.tag: ww_surv_target_1
attributes.value: SARS-CoV-2
attributes.tag: ww_surv_target_1_gene
attributes.value: N gene (N2)
attributes.tag: ww_surv_target_1_known_present
attributes.value: yes
attributes.tag: ww_surv_target_1_conc
attributes.value: 322253.59
attributes.tag: ww_surv_target_1_conc_unit
attributes.value: copies/L wastewater
attributes.tag: ww_surv_target_1_protocol
attributes.value: ddPCR detection of SARS-CoV-2 using the BioRad PREvalence Kit on the BioRad QX200
attributes.tag: ww_surv_target_2
attributes.value: SARS-CoV-2
attributes.tag: ww_surv_target_2_gene
attributes.value: E gene (orf4)
attributes.tag: ww_surv_target_2_known_present
attributes.value: yes
attributes.tag: ww_surv_target_2_conc
attributes.value: 193760.82
attributes.tag: ww_surv_target_2_conc_unit
attributes.value: copies/L wastewater
attributes.tag: ww_surv_target_2_protocol
attributes.value: ddPCR detection of SARS-CoV-2 using the BioRad PREvalence Kit on the BioRad QX200
attributes.tag: gisaid_accession
attributes.value: EPI_ISL_19876431
attributes.tag: gisaid_virus_name
attributes.value: hCoV-19/env/USA/PA-PHL2-VH-Merged-F02-0207/2025
attributes.tag: collection_date
attributes.value: 2025-02-05
attributes.tag: BioSampleModel
attributes.value: SARS-CoV-2: wastewater surveillance