title: human gut microbiome
organism: Homo sapiens
attributes.tag: isolate
attributes.value: stool
attributes.tag: age
attributes.value: 20
attributes.tag: biomaterial_provider
attributes.value: Universidade Federal da Bahia, Laboratrio de Imunofarmacologia e Biologia Molecular (IMUNOBIO)
attributes.tag: collection_date
attributes.value: 2018-07-05
attributes.tag: geo_loc_name
attributes.value: Brazil: Salvador
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: gut
attributes.tag: library_id
attributes.value: ISP.BR4330
attributes.tag: title
attributes.value: human gut microbiome
attributes.tag: library_strategy
attributes.value: AMPLICON
attributes.tag: library_source
attributes.value: METAGENOMIC
attributes.tag: library_selection
attributes.value: PCR
attributes.tag: library_layout
attributes.value: paired
attributes.tag: platform
attributes.value: ILLUMINA
attributes.tag: instrument_model
attributes.value: Illumina MiSeq
attributes.tag: design_description
attributes.value: 16S amplicon library preparation and sequencing were conducted following the Earth Microbiome Protocol (https://earthmicrobiome.org/). PCR amplification of the extracted DNA was performed for 30 cycles targeting the V4 region of the 16S rRNA gene. Universal primers 515F and 806R (515F: GTGCCAGCMGCCGCGGTAA; 806R: GGACTACHVGGGTWTCTAAT) were used with a Golay barcode on the forward primer.
attributes.tag: filetype
attributes.value: fastq
attributes.tag: BioSampleModel
attributes.value: Human