organism: human metagenome
attributes.tag: Library ID
attributes.value: adjacent_5R-16S_024
attributes.tag: sample_type
attributes.value: 5R-16S rDNA sequencing of Homo sapiens: melanoma tumor and control tissues
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: collection_date
attributes.value: not provided
attributes.tag: isolate
attributes.value: not provided
attributes.tag: strain
attributes.value: not provided
attributes.tag: geographic location
attributes.value: China
attributes.tag: Library strategy
attributes.value: AMPLICON
attributes.tag: Library source
attributes.value: GENOMIC
attributes.tag: Library selection
attributes.value: Hybrid Selection
attributes.tag: Library layout
attributes.value: paired
attributes.tag: Platform
attributes.value: ILLUMINA
attributes.tag: instrument model
attributes.value: Illumina NovaSeq 6000
attributes.tag: Desigm description
attributes.value: Samples were collected and processed using the CTAB method. Negative controls, including sampling, DNA extraction, and no-template PCR amplification controls, were included to account for contamination at various stages.16S rRNA gene amplification targeted 5 regions in multiplex. The libraries were sequenced on the Illumina NovaSeq 6000 system. Reads were demultiplexed, filtered, and aligned to the amplified regions. SMURF method was used for profiling. GreenGenes (May 2043 version) was used as a reference. Contamination was detected and removed following the specified filters.
attributes.tag: Filetype
attributes.value: fastq
attributes.tag: Filename
attributes.value: adjacent_24_R1.fastq.gz
attributes.tag: Filename
attributes.value: adjacent_24_R2.fastq.gz
attributes.tag: env_broad_scale
attributes.value: missing
attributes.tag: env_local_scale
attributes.value: missing
attributes.tag: env_medium
attributes.value: missing
attributes.tag: isol_growth_condt
attributes.value: missing
attributes.tag: lat_lon
attributes.value: missing
attributes.tag: BioSampleModel
attributes.value: MIMARKS.specimen
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.human-associated