organism: Homo sapiens
attributes.tag: isolate
attributes.value: missing
attributes.tag: age
attributes.value: 14
attributes.tag: biomaterial_provider
attributes.value: American Type Culture Collection (ATCC)
attributes.tag: collection_date
attributes.value: missing
attributes.tag: geo_loc_name
attributes.value: missing
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: peripheral blood
attributes.tag: cell_line
attributes.value: Jurkat
attributes.tag: cell_subtype
attributes.value: Clone E6-1
attributes.tag: cell_type
attributes.value: T lymphocyte
attributes.tag: disease
attributes.value: acute T cell leukemia
attributes.tag: vcn
attributes.value: Three integrated copies of CD19 CAR LVV in Jurkat genome. The integrated VCN was analyzed by ddPCR and NGS assays. Six LVV components from ddPCR assays were analyzed to determine the VCNs: LTR, Psi, RRE, CD19 CAR, eGFP. Three copies of most elements, and an extra copy of WPRE were detected in VCN3 cells. VCN2-5 were all derived from VCN1and have one copy of proviral DNA inserted on chromosome (chr) 17 (78,730,345, minus strand). However, there was a 724 bp deletion within this proviral DNA sequence in the EF1a promoter region. VCN3 had an additional insertion on chrX (103,930,456, minus strand) and another copy inserted on chr1 (23,307,863, minus strand) with a 789 bp repeat (part of the WPRE sequence) at the end of 3 LTR sequence, contained 1 SNP. NGS also revealed that there was a 260 bp random insertion on chr8 (99,830,407, minus strand), composed mostly from LTR sequence.
attributes.tag: BioSampleModel
attributes.value: Human