title: CO-CL-YO-05
organism: soil metagenome
attributes.tag: ENA first public
attributes.value: 2025-09-11
attributes.tag: External Id
attributes.value: SAMEA114349213
attributes.tag: INSDC center name
attributes.value: Scotland's Rural College - Aberdeen Campus
attributes.tag: INSDC last update
attributes.value: 2023-09-11T17:50:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: ms00050
attributes.tag: adapters
attributes.value: Forward overhang: 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG; Reverse overhang: 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG
attributes.tag: broad-scale environmental context
attributes.value: environmental system determined by an organism
attributes.tag: chimera check software
attributes.value: N/A
attributes.tag: climate environment
attributes.value: controlled glasshouse
attributes.tag: collection date
attributes.value: 2021-12-30
attributes.tag: environmental medium
attributes.value: bulk soil
attributes.tag: experimental factor
attributes.value: bulk soil no plant control
attributes.tag: geographic location (country and/or sea)
attributes.value: United Kingdom
attributes.tag: geographic location (latitude)
attributes.value: 51.8094
attributes.tag: geographic location (longitude)
attributes.value: 0.3561
attributes.tag: host age
attributes.value: 3
attributes.tag: host common name
attributes.value: N/A
attributes.tag: host life stage
attributes.value: N/A
attributes.tag: host subspecific genetic lineage
attributes.value: N/A
attributes.tag: host taxid
attributes.value: 410658
attributes.tag: local environmental context
attributes.value: soil environment
attributes.tag: multiplex identifiers
attributes.value: N/A
attributes.tag: negative control type
attributes.value: N/A
attributes.tag: nucleic acid amplification
attributes.value: https://emea.support.illumina.com/downloads/16s_metagenomic_sequencing_library_preparation.html
attributes.tag: nucleic acid extraction
attributes.value: Qiagen(TM) Power soil gDNA extraction kit
attributes.tag: pcr conditions
attributes.value: 95�C for 3 minutes; 25 cycles of 95�C for 30 seconds, 55�C for 30 seconds, 72�C for 30 seconds; 72�C for 5 minutes; Hold at 4�C
attributes.tag: pcr primers
attributes.value: Forward: 5' CCTACGGGNGGCWGCAG; Reverse: 5' GACTACHVGGGTATCTAATCC
attributes.tag: plant body site
attributes.value: N/A
attributes.tag: plant product
attributes.value: N/A
attributes.tag: positive control type
attributes.value: N/A
attributes.tag: project name
attributes.value: UK Crop Microbiome Cryobank
attributes.tag: reference for biomaterial
attributes.value: Rothamsted Research Station
attributes.tag: relevant electronic resources
attributes.value: agmicrobiomebase.org
attributes.tag: sample name
attributes.value: ms00050
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 0.5
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sequence quality check
attributes.value: software
attributes.tag: sequencing method
attributes.value: MiSeq
attributes.tag: target gene
attributes.value: 16S