organism: Homo sapiens
description: Protocols: GBM cells (P) were grown to 70-80% confluency and subjected to their respective lethal doses of radiation U87MG (10Gy) and PS2 (14Gy). After an initial death phase, a transiently non-proliferative residual population (RS) was conformed microscopically and by monitoring cell proliferation by trypan blue method. The residual cells which resumed proliferation were designated as end of residual (ERS). Cell population after complete recovery was designated as relapse (R).  RNA Isoplus (Takara) was used for isolation and enrichment of total RNA from parent, residual, end of residual and relapse GBM samples. TruSeq RNA library protocol (Illumina) was used for generation of libraries. Post QC verification, 4 μg of intact total RNA was used for mRNA purification through oligodT beads, and library preparation was carried out according to manufacturer's guidelines using the TruSeq RNA Sample Preparation Kit (Illumina).