organism: Homo sapiens
description: Protocols: K562-t(7;12) cells suspensions treated with each compounds were centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction. Total RNA was extracted using Monarch Total RNA Miniprep Kit (NEB). Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 400 ng (20 ng/μl) of high-quality total RNA was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.