organism: Mus musculus
description: This study aims to characterize the gut microbiota of mice using 16S rRNA gene (V3-V4 region) sequencing. Fecal samples were collected on postnatal day 28, and microbial DNA was analyzed via Illumina NovaSeq 6000. Through QIIME2 and DADA2 pipelines, we identified Amplicon Sequence Variants (ASVs) and annotated them against the Silva database. The project focuses on microbial community diversity (alpha/beta diversity) and inter-group differences, leveraging statistical tests (ANOVA, Kruskal-Wallis, etc.) to explore gut microbiota dynamics. Findings may inform research on host-microbe interactions and health outcomes.