organism: Ovis aries
description: Protocols: Samples of liver tissue were collected from the carcasses of the 12 lambs under study at the time of slaughter. These samples were preserved in an RNA-stabilizing solution (Ambion RNAlater; Life Technologies) and kept at 4°C for 24 hours. Afterward, the RNA-stabilizing solution was discarded, and the samples were frozen at −80°C until RNA extraction.  The extraction of RNA from the liver samples was conducted using a standard protocol for RNA extraction with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. The RNA integrity value was calculated using the Agilent 2,100 Bioanalyzer equipment (Agilent Technologies, CA, USA), which was higher than 7 for all the samples.  The cDNA library construction  was conducted at Novogene in Cambridge (UK) using the UltraTM RNA Library Prep Kit (NEBNext®, MA, USA).