organism: Homo sapiens
description: Protocols: Before library preparation, RNA concentration was evaluated through Qubit™ RNA High Sensitivity Assay Kit (Invitrogen, Walthman, MA, USA) while RNA quality was established using 4200 Tapestation (Agilent Technologies, Santa Clara, CA, USA).  Protocol: miRNeasy Mini Kit. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing. TruSeq Stranded Total RNA Sample Preparation Guide. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing.