organism: Temnothorax nylanderi
attributes.tag: isolate
attributes.value: Worker
attributes.tag: isolation_source
attributes.value: Taken from the wild, kept in lab conditions for 2 weeks before dissection
attributes.tag: collection_date
attributes.value: 2021-06-22
attributes.tag: geo_loc_name
attributes.value: Germany: Lennebergwald Mainz
attributes.tag: tissue
attributes.value: Fat body
attributes.tag: library_id
attributes.value: V2F
attributes.tag: title
attributes.value: RNA-seq of Tenmnothorax nylanderi: fat body
attributes.tag: library_strategy
attributes.value: RNA-Seq
attributes.tag: library_source
attributes.value: TRANSCRIPTOMIC
attributes.tag: library_selection
attributes.value: Oligo-dT
attributes.tag: library_layout
attributes.value: paired
attributes.tag: platform
attributes.value: ILLUMINA
attributes.tag: instrument_model
attributes.value: Illumina NovaSeq 6000
attributes.tag: design_description
attributes.value: Uninfected ant, not LPS treated. We isolated the fat body from each worker by removing the gut and trachea from the first gaster segment, leaving only the fat cells, which were placed in a vial containing 50 l Trizol. Samples were frozen on dry ice and subsequently transferred to -80C. RNA was extracted using the Qiagen RNeasy extraction kit. We sent the samples to Novogene (Cambridge, UK) for mRNA library preparation using a NEBNext Ultra RNA Library Prep Kit. The libraries were sequenced on an Illumina Novaseq 6000 in paired-end 150 bp mode, yielding a minimum of 9 Gb per sample.
attributes.tag: filetype
attributes.value: fastq
attributes.tag: filename
attributes.value: V2F_1.fq
attributes.tag: filename2
attributes.value: V2F_2.fq
attributes.tag: BioSampleModel
attributes.value: Invertebrate