title: 15S-NT-30-O_S125
organism: soil metagenome
attributes.tag: Blank_number
attributes.value: NA
attributes.tag: collection_date
attributes.value: 2019
attributes.tag: depth
attributes.value: 0-0.062 m
attributes.tag: elev
attributes.value: 241 m
attributes.tag: env_broad_scale
attributes.value: subpolar coniferous forest biome [ENVO:01000250]
attributes.tag: env_local_scale
attributes.value: soil [ENVO:00001998]
attributes.tag: env_medium
attributes.value: forest soil [ENVO:00002261] | burned soil [ENVO:00005760]
attributes.tag: geo_loc_name
attributes.value: Canada: Northwest Territories
attributes.tag: lat_lon
attributes.value: 61.805135 N 116.447424 W
attributes.tag: annual_precpt
attributes.value: 330 mm
attributes.tag: annual_temp
attributes.value: -3 °C
attributes.tag: cur_land_use
attributes.value: conifers (e.g. pine,spruce,fir,cypress)
attributes.tag: cur_vegetation
attributes.value: Pj-Sb/feather moss
attributes.tag: cur_vegetation_meth
attributes.value: Beckingham, J. D., and J. H. Archibald. 1996. Fieldguide to ecosites of Northern Alberta. NaturalResources Canada, Canadian Forest Service,Northern Forestry Centre, Edmonton, Alberta,Canada.
attributes.tag: fire
attributes.value: yes
attributes.tag: horizon_meth
attributes.value: Subjective
attributes.tag: ph
attributes.value: 8.78
attributes.tag: ph_meth
attributes.value: 1:5 soil dry mass to DI water
attributes.tag: samp_collect_device
attributes.value: Metal core, 5.5 cm i.d., variable depth up to 13.5 cm
attributes.tag: samp_mat_process
attributes.value: Preserved in Lifeguard soil preservation solution, as cold as possible in field, then frozen, and finally -80°C
attributes.tag: samp_size
attributes.value: 250 mg
attributes.tag: soil_horizon
attributes.value: O horizon
attributes.tag: soil_type
attributes.value: Typic Mesisol
attributes.tag: soil_type_meth
attributes.value: Soil Classification Working Group. 1998. The Canadian System of Soil Classification, 3rd ed. Agriculture and Agri-Food Canada Publication 1646, 187 pp. ISBN 0-660-17404-50
attributes.tag: tot_n_meth
attributes.value: combustion elemental analysis
attributes.tag: tot_nitro
attributes.value: 0.354624845
attributes.tag: tot_org_c_meth
attributes.value: combustion elemental analysis
attributes.tag: tot_org_carb
attributes.value: 10.82321
attributes.tag: Sample_Name_Simple
attributes.value: 30-O
attributes.tag: Project_ID
attributes.value: WB19
attributes.tag: Sample_ID
attributes.value: 15S-NT-30O
attributes.tag: Site_ID
attributes.value: 15S-NT-30
attributes.tag: Fire_ID
attributes.value: 2014ZF-046
attributes.tag: Ecosite
attributes.value: c1.2
attributes.tag: Moisture
attributes.value: Mesic
attributes.tag: Moisture_Regime
attributes.value: 5
attributes.tag: Nutrient
attributes.value: Poor
attributes.tag: Community
attributes.value: Pj-Sb/feather moss
attributes.tag: Interval
attributes.value: Long
attributes.tag: Veg_Comm
attributes.value: Jack Pine
attributes.tag: Land_Class
attributes.value: Upland
attributes.tag: Burned_Unburned
attributes.value: Burned
attributes.tag: Understory_CBI_1_year_post_fire
attributes.value: 2.43
attributes.tag: Overstory_CBI_1_year_post_fire
attributes.value: 3
attributes.tag: CBI_1_year_post_fire
attributes.value: 2.65
attributes.tag: RBR_1_year_post_fire
attributes.value: 568.3528422
attributes.tag: Pct_Exposed_Mineral_1_year_post_fire
attributes.value: 4.522727273
attributes.tag: CFSI_1_year_post_fire
attributes.value: 4.061944444
attributes.tag: Burn_Severity_Index_1_year_post_fire
attributes.value: 3.794545455
attributes.tag: O_Depth_cm
attributes.value: 6.166666666666667
attributes.tag: Severity_Class
attributes.value: High
attributes.tag: nucl_acid_ext
attributes.value: Qiagen / MoBio PowerLyzer PowerSoil DNA extraction kit
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: pcr_primers
attributes.value: FWD: AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGTGTGYCAGCMGCCGCGGTAA REV: CAAGCAGAAGACGGCATACGAGATNNNNNNNNAGTCAGCCAGCCGGACTACNVGGGTWTCTAAT
attributes.tag: pcr_cond
attributes.value: initial denaturation:98_2; denaturation:98_0.5; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
attributes.tag: seq_meth
attributes.value: Illumina MiSeq 2x250 PE
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.soil