title: E-B28b
organism: soil metagenome
attributes.tag: biotic_regm
attributes.value: none
attributes.tag: chem_administration
attributes.value: none
attributes.tag: collection_date
attributes.value: 2021-06-22
attributes.tag: depth
attributes.value: surface
attributes.tag: env_broad_scale
attributes.value: anthropogenic terrestrial biome [ENVO_01000219]
attributes.tag: env_local_scale
attributes.value: farm [ENVO_00000078]
attributes.tag: env_medium
attributes.value: agricultural soil [ENVO_00002259]
attributes.tag: food_product_type
attributes.value: not applicable
attributes.tag: geo_loc_name
attributes.value: USA:Ohio
attributes.tag: ifsac_category
attributes.value: not applicable
attributes.tag: lat_lon
attributes.value: 40.7589 N 81.9535 W
attributes.tag: samp_mat_type
attributes.value: natural environment specimen [GENEPIO_0001250]
attributes.tag: adjacent_environment
attributes.value: farm [ENVO_00000078]
attributes.tag: collection_method
attributes.value: sterile scoop
attributes.tag: enrichment_protocol
attributes.value: FDA BAM Chapter 4A|https://www.fda.gov/food/laboratory-methods-food/bam-chapter-4a-diarrheagenic-escherichia-coli
attributes.tag: fertilizer_admin
attributes.value: animal manure [AGRO_00000079]
attributes.tag: fertilizer_date
attributes.value: 2021-02-02
attributes.tag: humidity
attributes.value: 68%
attributes.tag: isolation_source
attributes.value: manure amended agricultural soil
attributes.tag: perturbation
attributes.value: amendment;2021-02-02|tilling;2021-04-14
attributes.tag: ph
attributes.value: 6.4
attributes.tag: ph_meth
attributes.value: 1:1 in H20 McLean, E.O. 1982. Soil pH and lime requirement. p. 199-223. In A.L. Page et al. (ed.) Methods of soil analysis, part 2. Agronomy Monogr. 9, 2nd ed. ASA and SSSA, Madison, WI.
attributes.tag: purpose_of_sampling
attributes.value: research
attributes.tag: rel_location
attributes.value: from the crop-growing fields
attributes.tag: repository
attributes.value: US FDA
attributes.tag: samp_collect_device
attributes.value: sterile scoop
attributes.tag: samp_pooling
attributes.value: soil sample was a composite of 5 subsamples taken from the field
attributes.tag: samp_size
attributes.value: 2L
attributes.tag: samp_source_mat_cat
attributes.value: natural environment specimen [GENEPIO_0001250]
attributes.tag: samp_store_dur
attributes.value: 24-48 hours before enrichment
attributes.tag: samp_store_temp
attributes.value: 4 degrees during transport; -20 until processing
attributes.tag: samp_vol_we_dna_ext
attributes.value: 50g used for enrichment; 2ml enrichment used for DNA extraction
attributes.tag: season
attributes.value: summer
attributes.tag: season_humidity
attributes.value: 81.62%
attributes.tag: season_precpt
attributes.value: 0.35cm
attributes.tag: season_temp
attributes.value: 21.90C
attributes.tag: soil_ph
attributes.value: 6.4
attributes.tag: soil_temp
attributes.value: 21.3C
attributes.tag: soil_texture_class
attributes.value: silt loam
attributes.tag: soil_texture_meth
attributes.value: Hydrometer Method. ASTM D422, 2606
attributes.tag: timepoint
attributes.value: 2021-06-22
attributes.tag: tot_org_c_meth
attributes.value: water extractable organic carbon Nelson, D.W., and L.E. Sommers. 1996. Total carbon, organic carbon and organic matter. P. 961-1010. In J.M. Bartels et al. (ed.) Methods of soil analysis: Part 3 Chemical methods. (3rd ed.) ASA and SSSA Book Series 5, Madison, WI.
attributes.tag: tot_org_carb
attributes.value: .1848
attributes.tag: wind_direction
attributes.value: 247.2 degrees
attributes.tag: wind_speed
attributes.value: 2.4 kmph
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.food-farm_env