title: K562-EVI1-GFP cells with EVI1 knockout (single-cell clone #2)
organism: Homo sapiens
attributes.tag: sample_name
attributes.value: K562_EVI1-KO2
attributes.tag: age
attributes.value: 53
attributes.tag: biomaterial_provider
attributes.value: JCRB Cell Bank
attributes.tag: cell_line
attributes.value: K562
attributes.tag: cell_type
attributes.value: myeloid leukemia cell
attributes.tag: collection_date
attributes.value: missing: lab stock
attributes.tag: culture_collection
attributes.value: JCRB:0019
attributes.tag: disease
attributes.value: chronic myelogenous leukemia
attributes.tag: disease_stage
attributes.value: blast crisis
attributes.tag: geo_loc_name
attributes.value: missing: lab stock
attributes.tag: isolate
attributes.value: clone 3F9
attributes.tag: phenotype
attributes.value: EVI1 knockout, no significant change in cell proliferation
attributes.tag: race
attributes.value: Caucasian
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: pleural effusion
attributes.tag: treatment
attributes.value: genetic modification: Cas9-mediated P2A-EGFP insertion at the EVI1 stop codon, lentiviral transduction of Cas9 and EVI1-targeting sgRNA #5 (TGTGGGTGAAACAAGAATCC), followed by selection for single-cell clones where EVI1 knockout was confirmed
attributes.tag: BioSampleModel
attributes.value: Human