title: s_NC2
organism: Bos taurus
attributes.tag: source_name
attributes.value: Transfection of NC cells
attributes.tag: cell type
attributes.value: Transfection of NC cells
attributes.tag: treatment
attributes.value: Cell transfection: After the adipocytes were cultured to the best state and digested, each group of cells was counted and prepared into 1.0×10^5 cells-mL-1, 2 mL of non-antimicrobial medium was added to each well of a six-well plate, and 130 µL of cells were spread into each well, and cultured overnight at 37°C in a 5% CO2 incubator; after 12-20 h, observe that the cell fusion rate reaches 70%~90%, and then transfect; prepare NC plasmid and transfection reagent diluents. After 12-20 h, observe that the cell fusion rate reaches 70%~90% for transfection; prepare plasmid and transfection reagent dilutions. Mix the transfection reagents well and incubate at room temperature for 5 minutes; transfer liquid A to liquid B and mix well, let it stand at room temperature for 15 minutes; transfer the cells into 6-well plates with 1000 µL of opti/MEMI pre-filled, and change the medium to complete medium after 6 hours; after 48 hours of transfection, harvest the cells with TRIZOL and use them for the next step directly, or store them in the refrigerator at -80℃ for spare parts. After 48 h of transfection, cells were harvested by TRIZOL and then used directly in the next experiment or stored in -80℃ refrigerator.
attributes.tag: geo_loc_name
attributes.value: missing
attributes.tag: collection_date
attributes.value: missing