organism: Homo sapiens
description: The cells were treated with 50μM Ac4ManNAz for 48h, then the RNA is extracted, treated with proteinase K, and subsequently separated by sucrose density gradient centrifugation to isolate RNA components smaller than approximately 2000 nt.The RNA were conjugated with DBCO-PEG4-biotin reagent.We reserved 150 ng of the conjugated RNA as the input sample, whereas 25 μg of the conjugated RNA was used as the enrich sample for enrichment via streptavidin magnetic beads. After that, both the input and the enrich RNA were used to construct libraries and sequenced using the Illumina NovaSeq PE150 platform.