title: 12hAPF
organism: Drosophila melanogaster
description: Protocols: Cells were dissociated using a protocol adapted from Harzer et al (doi: 10.1038/nprot.2013.062). A FACS sorting was performed to isolate GFP+ cells. A pool of leg discs was collected for each developmental time point. T1 leg discs were dissected in less than 1 hour to ensure tissue integrity, in M3 medium. Crosses were performed on day 1. An egg collection of 3 hours was made the next day. The flies were raised at 25°C. MPs/myoblasts were labeled with a membrane GFP under the control of mef2-Gal4 transgene. None - Included in nucleic acid library construction protocol. Chromium Single Cell 3' Reagent Kit version v3.1; Single index