title: Sample 5
organism: Mus musculus
description: Protocols: Mice aged 3 or 18 months old underwent terminal isoflurane anesthesia followed by exsanguination. The brain (including cerebellum and brain stem) was harvested and hemisected longitudinally; the right side was mechanically pulverized (Qiagen TissueLyser II). The New England Biolabs Monarch Total RNA Miniprep Kit was used to isolate RNA and perform DNAse I treatment. RNA-seq was performed by the University of Leeds Next Generation Sequencing core facility (Illumina NextSeq 2000) to acquire 150 base pair single-end reads.