title: FaLH03 3 days GYEP and 4 days alkaline low nitrogen medium
organism: Fusarium avenaceum
description: grown for 3 days in GYEP media, then fungal biomass transferred to 50 ml of an alkaline low nitrogen media (modified 2nd stage media with added sodium nitrate [0.05 g (NH4)2HPO4, 0.3 g KH2PO4, 0.02 g MgSO4-7H2O, 0.5 g NaCl, 4 g sucrose, 1 g glycerol, 600 l 1M NaNO3 + 100 mL of distilled water]) for 4 days of growth at 28 C, 170 rpm in the dark, then fungal biomass immediately harvested and flash frozen in liquid nitrogen.