title: HBMEC2-10M1T
organism: Homo sapiens
description: Protocols: Samples were collected directly in the well using a lysing solution (1% 2-mercaptoethanol + Buffer RLT; RNeasy Mini Kit, 74106, Qiagen). Study 1: Human brain microvascular endothelial cells (HBMEC) and human dermal microvascular endothelial cells (HDMEC) were stimulated with either 0ng/mL, 1ng/mL or 10ng/mL of TNF for 0 hours, 10 minutes (0.167 hours), 30 minutes (0.5 hours), 2 hours, or 6 hours. Study 2: HBMEC were stimulated with either 1ng/mL or 10ng/mL of TNF for 0 hours, 6 hours, 12 hours, or 24 hours. RNeasy mini kit (Qiagen, 74106) buffers and protocol. RNA-sequencing libraries were prepared using the TruSeq Stranded mRNA kit (20020595, Illumina following the manufacturers protocol.