title: Sample 1
organism: Mus musculus
description: Protocols: The mouse iPSC were a gift from Hans Schöler lab: Velychko, S., Adachi, K., Kim, K.-P., Hou, Y., MacCarthy, C.M., Wu, G., and Schöler, H.R. (2019). Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem Cellhttps://doi.org/10.1016/j.stem.2019.10.002.ROSA26-rtTA/GOF18Briefly, mouse embryonic fibroblasts of passage 3 or 4 were reprogrammed by lentiviral infection with a dox inducible cassette expressing Sox2/Klf4/c-Myc. Strain is a mixed background C57BL/6 and 129. Cells were trypsinized and FACS sorted to select live and single cells and to exclude feeder cells colored in red. Cells were washed twice and passed through a cell strainer 35um before final counting and viability assessment with an automatic cell counter. Chromium Singe Cell 3' v3, 10x Genomics Chromium Singe Cell 3' v3, 10x Genomics