title: EXT386
organism: Mus musculus
description: Protocols: Estrous cycle phase was determined by vaginal smear.14-15 weeks old mice were euthanized by CO2 asphyxiation, followed by uterine horn dissection. The uterine horns were longitudinally opened to expose the endometrium and cut into small pieces. Subsequently, these uterine horn pieces were dissociated enzymatically in 1% collagenase for 60 minutes at 37°C. Afterwards, the endometrial tissue pieces were mechanically dissociated by pipetting up and down for 10 minutes. 10% FBS was added to stop the enzymatic reaction. The cell suspension was filtered through a 40 μm cell strainer. The uterus single-cell suspensions were converted to barcoded Drop-seq libraries by using the Chromium Single Cell 3' V3.1 Library, Gel Bead & Multiplex Kit and Chip Kit (10X Genomics; Pleasanton, California, USA), aiming for an estimated 8,000 cells per library.  Single-cell libraries were prepared with the Chromium Single Cell 3' V3.1 Chemistry Library Kit, Gel Bead & Multiplex Kit and Chip Kit from 10X Genomics.