title: Sample 6- S62
organism: Homo sapiens
description: Protocols: Human fetal cortical neurons were cultured under standard conditions and treated with alpha-synuclein preformed fibrils (PFFs) and/or peptide inhibitors as indicated. At defined time points, cells were collected and dissociated into single-cell suspensions using TrypLE (Thermo Fisher Scientific, 12605010) according to the manufacturer's instructions. Cells were washed and resuspended for downstream single-cell library preparation. RNA extraction was not performed separately. Instead, single-cell suspensions were directly processed for single-cell RNA-seq library preparation, where cell lysis and RNA capture were performed within the workflow according to the manufacturer's protocol. Single-cell RNA-seq libraries were prepared using a droplet-based platform (10x Genomics Chromium) following the manufacturer's protocol. Briefly, single cells were encapsulated with barcoded beads, and reverse transcription was performed to generate cDNA. Amplified cDNA was used for library construction, including fragmentation, end repair, adapter ligation, and PCR amplification.