title: inf1
organism: Leishmania infantum
description: Protocols: The parasites were cultured at 26°C under classical conditions in promastigote medium. Total RNA was extracted using Nucleospin RNA isolation kit (MACHEREY-NAGEL) from the Linf/Ltar hybrid and its parents, from three different biological replicates on the second day of growth culture, according to the manufacturer instructions Libraries were built using an Illumina Stranded mRNA preparation kit.