title: Wingless3
organism: Acyrthosiphon pisum
description: Protocols: Samples were collected from the crowding experiment that is repeat 7 times.  Each biological replicate represent seven adult aphid (pooled samples of 1 aphid from each crowding experiment). DNA were extracted using the Qiagen DNAeasy Blood and Tissue Kit Library were constructed using the Next Gen DNA library kit (Active motif) and each samples were added a specific index using the NextGen Indexing Kit