title: FaLH03 2 days GYEP and 3 days acidic high nitrogen medium
organism: Fusarium avenaceum
description: grown for 2 days in GYEP media, then fungal biomass transferred to 50 ml of an acidic high nitrogen media (modified 2nd stage media with added glutamine [0.05 g (NH4)2HPO4, 0.3 g KH2PO4, 0.02 g MgSO4-7H2O, 0.5 g NaCl, 4 g sucrose, 1 g glycerol, 30 mL 200 mM glutamine + 70 mL of distilled water]) for 3 days of growth at 28 C, 170 rpm in the dark, then fungal biomass immediately harvested and flash frozen in liquid nitrogen.