title: toti_illumina_v1_3_20220117_invitrogen_I3_R2
organism: gut metagenome
attributes.tag: ENA first public
attributes.value: 2025-07-06
attributes.tag: External Id
attributes.value: SAMEA114003841
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF SZEGED
attributes.tag: INSDC last update
attributes.value: 2023-07-06T21:05:10Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Can_fam_Toti_illumina_v1_3_20220117_invitrogen_I3_R2
attributes.tag: adapters
attributes.value: Perkin-Fw-(AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT), Perkin-Rev-(CAAGCAGAAGACGGCATACGAGATXXXXXXXXXXXX1GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT)
attributes.tag: broad-scale environmental context
attributes.value: Csongrad county
attributes.tag: collection date
attributes.value: 2021-12-01
attributes.tag: environmental medium
attributes.value: no
attributes.tag: geographic location (country and/or sea)
attributes.value: Hungary
attributes.tag: geographic location (latitude)
attributes.value: 46.253
attributes.tag: geographic location (longitude)
attributes.value: 20.14824
attributes.tag: host age
attributes.value: 13
attributes.tag: host body product
attributes.value: stool
attributes.tag: host common name
attributes.value: Hungarian herding terrier: Pumi
attributes.tag: host diet
attributes.value: omnivore
attributes.tag: host family relationship
attributes.value: Harsanyi-Tekergo Bator
attributes.tag: host scientific name
attributes.value: Canis lupus familiaris
attributes.tag: host sex
attributes.value: male
attributes.tag: host subspecific genetic lineage
attributes.value: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
attributes.tag: host taxid
attributes.value: 9615
attributes.tag: library construction method
attributes.value: NEXTflex 16S V1 - V3 Amplicon-Seq Kit - 12
attributes.tag: library screening strategy
attributes.value: PCR
attributes.tag: local environmental context
attributes.value: Szeged
attributes.tag: multiplex identifiers
attributes.value: AAATCTCAGGCC
attributes.tag: nucleic acid amplification
attributes.value: NEXTflex 16S V1 - V3 Amplicon-Seq Kit - 12
attributes.tag: nucleic acid extraction
attributes.value: PureLink Microbiome DNA Purification Kit, Invitrogen
attributes.tag: pcr conditions
attributes.value: initial denaturation:98degC_4min; denaturation=98degC_30sec; annealing=60deg_30sec; extension=72deg_20sec
attributes.tag: pcr primers
attributes.value: 16S-V1-V3-Fw(CTCTTTCCCTACACGACGCTCTTCCGATCTAGAGTTTGATCCTGGCTCAG), 16S-V1-V3-Rev(CTGGAGTTCAGACGTGTGCTCTTCCGATCTGTATTACCGCGGCTGCTGG)
attributes.tag: project name
attributes.value: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes
attributes.tag: sample name
attributes.value: Can_fam_Toti_illumina_v1_3_20220117_invitrogen_I3_R2
attributes.tag: scientific_name
attributes.value: gut metagenome
attributes.tag: sequence quality check
attributes.value: software
attributes.tag: sequencing method
attributes.value: Illumina MiSeq v2 300 nano
attributes.tag: target gene
attributes.value: 16SrRNA
attributes.tag: target subfragment
attributes.value: V1-V3