title: toti_illumina_v3_4_20220621_zymo_Z2_R2
organism: gut metagenome
attributes.tag: ENA first public
attributes.value: 2025-07-06
attributes.tag: External Id
attributes.value: SAMEA114003923
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF SZEGED
attributes.tag: INSDC last update
attributes.value: 2023-07-06T21:05:11Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Can_fam_Toti_illumina_v3_4_20220621_zymo_Z2_R2
attributes.tag: adapters
attributes.value: Quick-16S-AdapterFw-(TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG), Quick-16S-AdapterRev-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG and Illumina P5 5'-(AATGATACGGCGACCACCGA), P7 5'-(CAAGCAGAAGACGGCATACGAGAT)
attributes.tag: broad-scale environmental context
attributes.value: Csongrad county
attributes.tag: collection date
attributes.value: 2021-12-01
attributes.tag: environmental medium
attributes.value: no
attributes.tag: geographic location (country and/or sea)
attributes.value: Hungary
attributes.tag: geographic location (latitude)
attributes.value: 46.253
attributes.tag: geographic location (longitude)
attributes.value: 20.14824
attributes.tag: host age
attributes.value: 13
attributes.tag: host body product
attributes.value: stool
attributes.tag: host common name
attributes.value: Hungarian herding terrier: Pumi
attributes.tag: host diet
attributes.value: omnivore
attributes.tag: host family relationship
attributes.value: Harsanyi-Tekergo Bator
attributes.tag: host scientific name
attributes.value: Canis lupus familiaris
attributes.tag: host sex
attributes.value: male
attributes.tag: host subspecific genetic lineage
attributes.value: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
attributes.tag: host taxid
attributes.value: 9615
attributes.tag: library construction method
attributes.value: Quick-16S NGS Library Prep Kit V3-V4
attributes.tag: library screening strategy
attributes.value: PCR
attributes.tag: local environmental context
attributes.value: Szeged
attributes.tag: multiplex identifiers
attributes.value: 3-(CATGATGC), 5-(CCGATCTT)
attributes.tag: nucleic acid amplification
attributes.value: Quick-16S NGS Library Prep Kit V3-V4
attributes.tag: nucleic acid extraction
attributes.value: Quick-DNA HMW MagBead Kit, Zymo
attributes.tag: pcr conditions
attributes.value: initial denaturation:95degC_10min; denaturation=95degC_30sec; annealing=55deg_30sec; extension=72deg_3min
attributes.tag: pcr primers
attributes.value: 341fw (CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG), 806rev (GACTACNVGGGTMTCTAATCC)
attributes.tag: project name
attributes.value: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes
attributes.tag: sample name
attributes.value: Can_fam_Toti_illumina_v3_4_20220621_zymo_Z2_R2
attributes.tag: scientific_name
attributes.value: gut metagenome
attributes.tag: sequence quality check
attributes.value: software
attributes.tag: sequencing method
attributes.value: Illumina MiSeq v2 500 nano
attributes.tag: target gene
attributes.value: 16SrRNA
attributes.tag: target subfragment
attributes.value: V3-V4