organism: Solanum lycopersicum
attributes.tag: biological material ID
attributes.value: 2218DP312
attributes.tag: biological material elevation
attributes.value: 112m
attributes.tag: biological material geographic location
attributes.value: Germany
attributes.tag: biological material latitude
attributes.value: 51,816
attributes.tag: biological material longitude
attributes.value: 11,283
attributes.tag: biological material ploidy
attributes.value: diploid
attributes.tag: checklist
attributes.value: BSDC00002
attributes.tag: collection date
attributes.value: 2022-05-17
attributes.tag: genotype
attributes.value: Moneymaker
attributes.tag: genus
attributes.value: Solanum
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth facility
attributes.value: phytochamber
attributes.tag: library type
attributes.value: RNAseq
attributes.tag: light regimen
attributes.value: 16 h day/8 h night
attributes.tag: material source altitude
attributes.value: not applicable
attributes.tag: material source description
attributes.value: not applicable
attributes.tag: material source geographic location
attributes.value: not collected
attributes.tag: material source latitude
attributes.value: not collected
attributes.tag: material source longitude
attributes.value: not collected
attributes.tag: plant age
attributes.value: 21 days
attributes.tag: plant anatomical entity
attributes.value: leaf
attributes.tag: plant structure development stage
attributes.value: leaf production stage
attributes.tag: project name
attributes.value: Tomato heat stress response
attributes.tag: replicate
attributes.value: 4
attributes.tag: sample ID
attributes.value: IPK_LIMS:3167104
attributes.tag: sample description
attributes.value: Seeds were planted in well-watered soil trays and kept under a plastic cover in a phytochamber with a day/night cycle of 16/8h and temperatures of 24°C and 20°C respectively. The seeds were kept without light for one day, then illumination of 320µmol per square meter per second was added during the day phase. After a total of 7 days after sowing, evenly germinated and healthy seedlings were transplanted into individual pots (10 cm diameter, 8 cm height) without plastic covers at 60-70% relative air humidity. After 9 further days of establishment in the pot, the plants were exposed to heat stress at 37°C/28°C (day/night) for 6 days followed by a recovery phase at 24°C/20°C. Plants were watered once or twice daily by an automated system replenishing evapotranspirated water by weight to ensure the plants do not experience drought stress. The youngest fully formed leaf pair was harvested (directly put in liquid nitrogen and then stored at -80°C) for molecular measurements mid-day (between 13:00 and 14:00, light phase was from 06:00-22:00) from individual plants directly before the temperature switch (day0), each day during the heat stress phase (day1 to day6) and after 1, 2, and 4 days of recovery (day7 to day10). Control plants were grown exactly the same way except that they stayed at 24°C/20°C throughout the whole experiment. They were sampled at the same respective time points as the heat-treated plants.
attributes.tag: sample name
attributes.value: Moneymaker_heat_day6_plant2218DP312
attributes.tag: species
attributes.value: lycopersicum
attributes.tag: temperatur regimen
attributes.value: 24 °C/20 °C
attributes.tag: treatment
attributes.value: heat stress