organism: Secale cereale
attributes.tag: ENA first public
attributes.value: 2026-03-11
attributes.tag: INSDC status
attributes.value: public
attributes.tag: accession_name
attributes.value: not collected
attributes.tag: age
attributes.value: 2 weeks
attributes.tag: air temperature regimen
attributes.value: 26°C day/21°C night
attributes.tag: biological material ID
attributes.value: Lo7_rep2
attributes.tag: biological material altitude
attributes.value: 112m
attributes.tag: biological material coordinates uncertainty
attributes.value: 2000m
attributes.tag: biological material geographic location
attributes.value: Germany
attributes.tag: biological material latitude
attributes.value: 51.816
attributes.tag: biological material longitude
attributes.value: 11.283
attributes.tag: biological material preprocessing
attributes.value: not applicable
attributes.tag: biological_material_doi
attributes.value: not collected
attributes.tag: biological_material_id
attributes.value: Lo7_rep2
attributes.tag: biological_material_ploidy
attributes.value: diploid
attributes.tag: checklist
attributes.value: ERC000011
attributes.tag: collection date
attributes.value: not collected
attributes.tag: genus
attributes.value: Secale
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: growth facility
attributes.value: growth chamber
attributes.tag: infraspecific_name
attributes.value: not collected
attributes.tag: ipk_sequencing_date
attributes.value: 2026-01-14
attributes.tag: light regimen
attributes.value: 16/8-hour light
attributes.tag: material source geographic location
attributes.value: Germany
attributes.tag: material_source_altitude
attributes.value: not collected
attributes.tag: material_source_description
attributes.value: not collected
attributes.tag: material_source_doi
attributes.value: not collected
attributes.tag: material_source_geographic_location
attributes.value: not collected
attributes.tag: material_source_id
attributes.value: not collected
attributes.tag: material_source_latitude
attributes.value: not collected
attributes.tag: material_source_longitude
attributes.value: not collected
attributes.tag: plant structure development stage
attributes.value: seedling development stage
attributes.tag: plant_anatomical_entity
attributes.value: leaf
attributes.tag: sample name
attributes.value: Lo7_rep2
attributes.tag: sample_description
attributes.value: The sample material for molecular analysis consisted of 2-week-old leaves from an individual Lo7 plant. All the plants were grown in soil in a growth chamber at 26 °C / 21 °C (day / night) for 24 days with a 16 h light/8 h dark regime (light intensity was 300 μmol m-2 sec-1, the relative humidity was kept at 71 %). DNA was extracted with DNeasy Plant Pro Kit (Qiagen), bisulfite-treated with the EZ DNA Methylation-Gold Kit (Zymo Research), and prepared for sequencing with the xGen Methyl-Seq Lib Prep Kit (IDT DNA), according to standard protocols from the manufacturers. Unmethylated Lambda DNA (Promega) was added as a spike-in to verify bisulfite conversion efficiency. Libraries were sequenced (paired-end, 2x151 cycles, Illumina NovaSeq X Plus, IPK Gatersleben) following protocols provided by the manufacturer (Illumina).
attributes.tag: sample_id
attributes.value: IPK_LIMS:3423433
attributes.tag: species
attributes.value: cereale