title: Amplicons of V3-V4 region of 16S rRNA gene from marine water metagenome, 2022, Barents Sea site 3
organism: seawater metagenome
attributes.tag: collection_date
attributes.value: 2022-08-12
attributes.tag: env_broad_scale
attributes.value: Aquatic biome [ENVO_00002030]
attributes.tag: env_local_scale
attributes.value: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]
attributes.tag: env_medium
attributes.value: Sea water [ENVO_00002149]
attributes.tag: geo_loc_name
attributes.value: Russia: Dalniye Zelentsy, Krasnaya skala, Guba Yarnyshnaya, Barents Sea
attributes.tag: host
attributes.value: Not applicable
attributes.tag: lat_lon
attributes.value: 69.11549 N 36.05281 E
attributes.tag: depth
attributes.value: 0 m
attributes.tag: host_common_name
attributes.value: Not applicable
attributes.tag: host_life_stage
attributes.value: Not applicable
attributes.tag: host_subject_id
attributes.value: 4799
attributes.tag: samp_collect_device
attributes.value: Manual picking
attributes.tag: samp_mat_process
attributes.value: Bacteria were collected on 0.22 mkm filter. A V3-V4 region of 16S rRNA gene was amplified using standard degenerate primers fused with sequencing adapters (Illumina). PCR Forward Primer = CCTACGGGNGGCWGCAG, PCR Reverse Primer = GACTACHVGGGTATCTAATCC, adapter sequences are not shown.
attributes.tag: samp_size
attributes.value: 3 L
attributes.tag: samp_vol_we_dna_ext
attributes.value: 3 L
attributes.tag: temp
attributes.value: 13.5 C
attributes.tag: size_frac
attributes.value: 0.22 mkm
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated