title: delta-NCU05033
organism: Neurospora crassa
attributes.tag: strain
attributes.value: FGSC #14738
attributes.tag: reference_material
attributes.value: Culturing procedure: The liquid cultures were incubated at 25°C, constant light at 200 rpm in 24-deep well plates. repr à de-repr. 24h in 2% Glucose VMM (harvesting of t0 directly after these 24h) 3x 15 min washing in 1x Vogel´s 1% Avicel VMM, harvested after the amount of time of each samping time point For the inoculation, fresh spores were used derived from slants incubated for 2 days at 30°C in the dark, then at 25°C at constant light for several days (~10 days in total), to avoid day:night cycling/ gene expression variations due to the clock controlled mechanism. Inoculation with 1*106 conidia/ml, in 3ml per well in 24-deep well plate format.
attributes.tag: collected_by
attributes.value: FGSC
attributes.tag: collection_date
attributes.value: Unknown
attributes.tag: geo_loc_name
attributes.value: Unknown
attributes.tag: isolation_source
attributes.value: Unknown
attributes.tag: source_type
attributes.value: other
attributes.tag: purpose_of_sampling
attributes.value: Unknown
attributes.tag: project_name
attributes.value: repr. to de-repr. t5
attributes.tag: BioSampleModel
attributes.value: One Health Enteric