organism: Triticum aestivum
attributes.tag: biological material ID
attributes.value: ITA383:AGENT_SSD_TA01017
attributes.tag: biological material ID
attributes.value: AGENT:AW_08758
attributes.tag: biological material altitude
attributes.value: not collected
attributes.tag: biological material coordinates uncertainty
attributes.value: not collected
attributes.tag: biological material geographic location
attributes.value: Italy
attributes.tag: biological material latitude
attributes.value: not collected
attributes.tag: biological material longitude
attributes.value: not collected
attributes.tag: biological material ploidy
attributes.value: hexaploid
attributes.tag: biological material preprocessing
attributes.value: This is a single seed descent (SSD) derived during 2023 from the accession number TA01017 maintained by CRA-Unitá di Ricerca per la Risicoltura, Vercelli, Italy
attributes.tag: checklist
attributes.value: BSDC00002
attributes.tag: collected by
attributes.value: not collected
attributes.tag: collection date
attributes.value: not collected
attributes.tag: genus
attributes.value: Triticum
attributes.tag: geographic location (country and/or sea)
attributes.value: Italy
attributes.tag: infraspecific name
attributes.value: Triticum aestivum
attributes.tag: material source ID
attributes.value: ITA383:TA01017
attributes.tag: material source altitude
attributes.value: not collected
attributes.tag: material source coordinates uncertainty
attributes.value: not collected
attributes.tag: material source description
attributes.value: not collected
attributes.tag: material source geographic location
attributes.value: Italy
attributes.tag: material source latitude
attributes.value: not collected
attributes.tag: material source longitude
attributes.value: not collected
attributes.tag: plant anatomical entity
attributes.value: leaf
attributes.tag: project name
attributes.value: AGENT - Activated Genebank Network
attributes.tag: record time IPK LIMS
attributes.value: 2023-09-29
attributes.tag: sample ID
attributes.value: IPK_LIMS:3220927
attributes.tag: sample description
attributes.value: Seeds from individual SSD lines were grown in a climatic chamber in 96-well trays for two weeks. Two leaf segments (each approximately 3 cm in length ) were placed in a 96-well polypropylene cluster tube (1.2 ml, 8 tube stripe format; Corning) containing one 2 mm lead bead. The tubes were closed with 8-cap tube strips. Samples were frozen in liquid nitrogen and stored at -80°C until use. Materials were grinded by Tissue Lizer (Quigen) and DNA was extracted with MATAB, cleaned with chloroform-isoamyl-alcohol 24:1, precipitated with isopropanol and washes with 70% Ethanol. After resuspending in Tris 10mM pH 8.4, DNAs were treated with RNAse A pancreatic, quantified by Qubit and checked for integrity by agarose gel electrophoresis.
attributes.tag: sample name
attributes.value: AW_08758
attributes.tag: species
attributes.value: aestivum