title: BACMIX_illumina_v1_v2_20221104_zymo_Z2_R2
organism: mixed culture metagenome
attributes.tag: ENA first public
attributes.value: 2025-07-06
attributes.tag: External Id
attributes.value: SAMEA114003973
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF SZEGED
attributes.tag: INSDC last update
attributes.value: 2023-07-06T22:50:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: BACMIX_illumina_v1_v2_20221104_zymo_Z2_R2
attributes.tag: adapters
attributes.value: Quick-16S-AdapterFw-(TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG), Quick-16S-AdapterRev-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG and Illumina P5 5'-(AATGATACGGCGACCACCGA), P7 5'-(CAAGCAGAAGACGGCATACGAGAT)
attributes.tag: annotation source
attributes.value: ZymoBIOMICS Microbial Community Standard Cat.Number: D6300
attributes.tag: broad-scale environmental context
attributes.value: Csongrad county
attributes.tag: collection date
attributes.value: 2021-12-01
attributes.tag: environmental medium
attributes.value: ZymoBIOMICS� Microbial Community Standard
attributes.tag: geographic location (country and/or sea)
attributes.value: Hungary
attributes.tag: geographic location (latitude)
attributes.value: 46.248755
attributes.tag: geographic location (longitude)
attributes.value: 20.145766
attributes.tag: local environmental context
attributes.value: Szeged
attributes.tag: multiplex identifiers
attributes.value: 5-(CATGATGC ) and 3-( CCGATCTT)
attributes.tag: organism count
attributes.value: 1.4 x 1010 cells/ml
attributes.tag: pcr conditions
attributes.value: initial denaturation:95degC_10min; denaturation=95degC_30sec; annealing=55deg_30sec; extension=72deg_3min
attributes.tag: positive control type
attributes.value: ZymoBIOMICS Microbial Community Standard
attributes.tag: project name
attributes.value: Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes
attributes.tag: relevant electronic resources
attributes.value: https://s3.amazonaws.com/zymo-files/BioPool/ZymoBIOMICS.STD.refseq.v2.zip
attributes.tag: sample name
attributes.value: BACMIX_illumina_v1_v2_20221104_zymo_Z2_R2
attributes.tag: scientific_name
attributes.value: mixed culture metagenome
attributes.tag: sequence quality check
attributes.value: software
attributes.tag: sequencing method
attributes.value: Illumina MiSeq v2 500 nano