title: ARMS_Cheshire_20191210_20220221_SF_R2
organism: metagenome
attributes.tag: ENA first public
attributes.value: 2026-04-27
attributes.tag: INSDC center name
attributes.value: European Marine Biological Resource Centre
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: EMOBON00760_18S
attributes.tag: ampl_size
attributes.value: 350
attributes.tag: arms_unit_id
attributes.value: Cheshire1
attributes.tag: broad-scale environmental context
attributes.value: marine benthic biome [ENVO_01000024]; natural environment [ENVO:01000951]
attributes.tag: collection date
attributes.value: 2022-02-21
attributes.tag: deployment_date
attributes.value: 2019-12-10
attributes.tag: depth
attributes.value: 20
attributes.tag: dna concentration
attributes.value: 5.57
attributes.tag: environmental medium
attributes.value: blended biomass derived from sessile communities colonising an autonomous reef monitoring structure [ENVO:01001863]
attributes.tag: geographic location (country and/or sea)
attributes.value: United Kingdom
attributes.tag: geographic location (latitude)
attributes.value: -67.57383333
attributes.tag: geographic location (locality)
attributes.value: Rothera Point
attributes.tag: geographic location (longitude)
attributes.value: -68.12523333
attributes.tag: geographic location (ocean)
attributes.value: Southern Ocean
attributes.tag: geographic location (region and locality)
attributes.value: Rothera Point
attributes.tag: ha_id
attributes.value: BAS Ha
attributes.tag: lib_layout
attributes.value: PAIRED
attributes.tag: library reads sequenced
attributes.value: 1268361
attributes.tag: local environmental context
attributes.value: marine subtidal rocky reef biome [ENVO:01000050], rocky reef [ENVO:00000130], artificial reef [ENVO:00000149]
attributes.tag: nucleic acid extraction
attributes.value: Quick-DNA Fecal/soil
attributes.tag: obs_id
attributes.value: BAS
attributes.tag: pcr primers
attributes.value: GCTTGTCTCAAAGATTAAGCC; CCTGCTGCCTTCCTTRGA
attributes.tag: pcr_blank_1
attributes.value: CEB_DEZ
attributes.tag: pcr_blank_2
attributes.value: CEB_DFA
attributes.tag: preservative
attributes.value: DESS
attributes.tag: project name
attributes.value: EMOBON
attributes.tag: quality_assessment
attributes.value: Pass
attributes.tag: relevant standard operating procedures
attributes.value: Prod_ILL_BqADN_SuperLowCost_150_v3
attributes.tag: replicate
attributes.value: 2
attributes.tag: resequencing
attributes.value: No
attributes.tag: samp_fraction
attributes.value: SF
attributes.tag: samp_site
attributes.value: Rothera
attributes.tag: sample collection device
attributes.value: autonomous reef monitoring structure [ENVO:01001855]
attributes.tag: sample material processing
attributes.value: ARMS Handbook v3.3
attributes.tag: sample name
attributes.value: EMOBON00760_18S
attributes.tag: samples_alias_orig
attributes.value: EMOBON00760
attributes.tag: sampling_event
attributes.value: Rothera_Cheshire_Ha_191210_220221
attributes.tag: scientific_name
attributes.value: metagenome
attributes.tag: sequence_batch
attributes.value: Batch3.2
attributes.tag: sequence_batch_rundate
attributes.value: Batch3.2_240409
attributes.tag: sequence_quality_check
attributes.value: basic Genoscope QC
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: source material identifiers
attributes.value: EMOBON_BAS_Ha_4
attributes.tag: source_mat_id_an
attributes.value: EMOBON_BAS_Ha_4_metab18SV1V2
attributes.tag: source_mat_id_orig
attributes.value: ARMS_Cheshire_20191210_20220221_SF_R2
attributes.tag: status
attributes.value: sample
attributes.tag: target gene
attributes.value: 18S rRNA
attributes.tag: target subfragment
attributes.value: V1-V2