title: wt_ctl_4
organism: Hordeum vulgare
description: Protocols: Tissue was collected from the middle part of the second leaf below the flag leaf in four biological replications. Each biological replication contained a fragment of leave from three independent plants. Seed were germinated on Petri dishes at 28°C in a growth chamber. After 5 days seedlings were transplanted to seedling pots and grown at 25°C. On the 20th day of growth plants were placed in 3L pots filled with a mixture of coarse sand with loam (1:3) and maintained under optimal water supply in greenhouse (22/16°C day/night, photoperiod 16/8h). 9 selected plants of each genotype were transferred to a PlantArray intelligent screening system (Plant-DiTech) on the 85th day of growth.  PlantArray lysimeter allows for strict monitoring of the water regimen and accurate drought application based on regular weight measurements and tracking of selected physiological parameters (Appiah et al. 2023). On the 99th day of growth (heading time, growth stage 50-59, Zadoks et al. 1974) 5 of the 9 plants of each genotype growing on a lysimeter were subjected to drought stress. Drought was applied for 15 days. After drought stress (flowering, growth stage 60-69, Zadoks et al. 1974) tissue collection for RNA-seq was performed. RNA was extracted using a mirVana RNA isolation kit (Thermo Fisher Scientific) according to the manufacturer's instructions. True Seq libraries were constructed for each sample. PE 150.
attributes.tag: ENA first public
attributes.value: 2025-08-01
attributes.tag: INSDC center name
attributes.value: Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14895:wt_ctl_4
attributes.tag: age
attributes.value: Growth stage 60-69, Zadoks scale
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: barley
attributes.tag: cultivar
attributes.value: Sebastian
attributes.tag: developmental stage
attributes.value: Heading/Flowering
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: Second leave below the flag leaf
attributes.tag: sample name
attributes.value: E-MTAB-14895:wt_ctl_4
attributes.tag: scientific_name
attributes.value: Hordeum vulgare
attributes.tag: stimulus
attributes.value: Control