title: Jemmeritz 1_006_R1S1 mtCOI metabarcoding from multiplexed PCR, NEB kit
organism: insect work metagenome
description: Metabarcoding of mtCOI long fragment with multiplex-PCR from eDNA extracted from debris from nesting aids deployed on agricultural landscapes in Germany. Simultaneous amplification of mtCOI and ITS2 fragments.
attributes.tag: ENA first public
attributes.value: 2025-08-01
attributes.tag: INSDC center name
attributes.value: Thuenen-Institute of Biodiversity
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Marker
attributes.value: NEB
attributes.tag: PrimerSeq_FWD_1
attributes.value: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
attributes.tag: PrimerSeq_FWD_2
attributes.value: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
attributes.tag: PrimerSeq_REV_1
attributes.value: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
attributes.tag: PrimerSeq_REV_2
attributes.value: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
attributes.tag: SampleID_ASV
attributes.value: F-HEAD-24-R-TAIL-3
attributes.tag: SampleName
attributes.value: 006
attributes.tag: Submitter Id
attributes.value: F-HEAD-24-R-TAIL-3.NEB.COI_lg
attributes.tag: TagFWD
attributes.value: CCAGTGC
attributes.tag: TagREV
attributes.value: GTCGCCA
attributes.tag: adapters
attributes.value: FWD: CCAGTGC REV: GTCGCCA
attributes.tag: broad-scale environmental context
attributes.value: ENVO:01000245
attributes.tag: collection date
attributes.value: 2020-10-09
attributes.tag: environmental medium
attributes.value: ENVO:02000004
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: 52.63508101
attributes.tag: geographic location (longitude)
attributes.value: 11.23317200
attributes.tag: lat
attributes.value: 52.6350810092732
attributes.tag: local environmental context
attributes.value: nest tube of cavity-nesting hymenoptera
attributes.tag: lon
attributes.value: 11.233172000596811
attributes.tag: multiplex identifiers
attributes.value: FWD: CCAGTGC REV: GTCGCCA
attributes.tag: nucleic acid amplification
attributes.value: PCR
attributes.tag: nucleic acid extraction
attributes.value: Machery-Nagel NucleoSpin 96 Soil Kit
attributes.tag: pcr primers
attributes.value: FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
attributes.tag: project name
attributes.value: PilotNA
attributes.tag: removal_date
attributes.value: 2020-10-09
attributes.tag: sample name
attributes.value: F-HEAD-24-R-TAIL-3.NEB.COI_lg
attributes.tag: scientific_name
attributes.value: insect work metagenome
attributes.tag: site
attributes.value: Jemmeritz
attributes.tag: site_ID
attributes.value: JE
attributes.tag: source material identifiers
attributes.value: 1_006_R1S1
attributes.tag: target gene
attributes.value: mtCOI
attributes.tag: target subfragment
attributes.value: long barcoding region
attributes.tag: trap_ID
attributes.value: 1_006_R1S1