title: Res_HFD_4
organism: Mus musculus
description: Protocols: Mouse epidydimal adipose tissue was harvested and digested in Hanks Balanced Salt Solution (HBSS,no calcium and magnesium) containing fetal bovine serum (FBS,3%) and collagenase D (2.5 mg/ml,Sigma-Aldrich) for 30 minutes at 37°C under agitation. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, the adipocyte fraction is discarded and the stroma vascular fraction passed through a 100 µm filter before staining. Cell suspensions were stained with appropriate antibodies for 30 min on ice. Cell sorting was performed on a BD FACSAria II™ cell sorter. RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen). cDNA libraries were generated using the KAPA mRNA HyperPrep kit (Roche).
attributes.tag: ENA first public
attributes.value: 2025-02-07
attributes.tag: External Id
attributes.value: SAMEA112484737
attributes.tag: INSDC center name
attributes.value: INSERM Sorbonne-Universite
attributes.tag: INSDC last update
attributes.value: 2023-02-07T16:32:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12596:Res_HFD_4
attributes.tag: age
attributes.value: 9
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: tissue-resident macrophage
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: diet
attributes.value: high fat diet
attributes.tag: disease
attributes.value: obesity
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: epididymal fat pad
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-12596:Res_HFD_4
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C3H