title: STZ-EAU
organism: Mus musculus
description: This sample consists of retinal single cells isolated from the retinas of streptozotocin (STZ)-induced C57BL/6J (B6) mice that were immunized to induce experimental autoimmune uveoretinitis (EAU) using human IRBP 1-20 (STZ-EAU). The sample preparation method is described in detail in the following reference: https://doi.org/10.3390/biomedicines13092049. Briefly, five-week-old female B6 mice were intraperitoneally administered 60 mg/kg of STZ for five consecutive days. Four weeks after the first injection, mice with blood glucose levels of 300 mg/dL or higher were used as diabetic mice. For EAU induction, the B6 mice were immunized at nine weeks of age by subcutaneous injection in the lumbar region with an emulsion of human IRBP 1-20 in PBS and CFA. To enhance the immune response, pertussis toxin was administered intraperitoneally. Three weeks post-immunization, the mice were euthanized and their retinas were enucleated. The retinas were then enzymatically digested with papain to obtain single-cell suspensions. Rod cells were subsequently removed using magnetic beads targeting CD73, and single-cell RNA sequencing was performed on the remaining retinal cells.
attributes.tag: sample_name
attributes.value: STZ-EAU
attributes.tag: age
attributes.value: 12_weeks
attributes.tag: biomaterial_provider
attributes.value: Japan SLC
attributes.tag: breed
attributes.value: missing
attributes.tag: cell_type
attributes.value: retinal cells
attributes.tag: collection_date
attributes.value: 2021-12-21
attributes.tag: cultivar
attributes.value: missing
attributes.tag: dev_stage
attributes.value: missing
attributes.tag: disease
attributes.value: Diabetes and experimental autoimmune uveoretinitis
attributes.tag: ecotype
attributes.value: missing
attributes.tag: geo_loc_name
attributes.value: Japan
attributes.tag: isolate
attributes.value: missing
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL6J
attributes.tag: tissue
attributes.value: retina