title: hVH1_2_hD3_3_hJH6_hVK1_33_CerSis_del_SP_IgH3
organism: Mus musculus
description: Protocols: Splenic B cells used for HTGTS-rep-seq were purified from unimmunized 5-8 weeks old mice by MACS® Microbeads according to the manufacturer's protocol. In brief, spleens were dissected out from unimmunized mice, prepared into single cell suspensions and stained with anti-B220 Microbeads for 20 minutes at 4°C. The splenic B cells were collected using the LS column and MACSTM Separater.  Genomic DNA was extracted from purified splenic B cells using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer's protocol. The libraries of HTGTS-Rep-seq was constructed as previous described (Lin et al., PNAS 2016)
attributes.tag: ENA first public
attributes.value: 2026-02-24
attributes.tag: INSDC center name
attributes.value: Boston Chidlren's Hospital
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16106:hVH1_2_hD3_3_hJH6_hVK1_33_CerSis_del_SP_IgH3
attributes.tag: age
attributes.value: 5-8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: B cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: hVH1-2/hD3-3-hJH6/hVK1-33_CerSis_del
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: spleen
attributes.tag: replicate
attributes.value: 3
attributes.tag: sample name
attributes.value: E-MTAB-16106:hVH1_2_hD3_3_hJH6_hVK1_33_CerSis_del_SP_IgH3
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6 and 129/Sv mix