title: Bageritz 9_099_R2S3 rbcL metabarcoding
organism: insect work metagenome
description: Metabarcoding of rbcL from eDNA extracted from debris in nesting aids deployed on agricultural landscapes in Germany
attributes.tag: ENA first public
attributes.value: 2025-08-01
attributes.tag: INSDC center name
attributes.value: Thuenen-Institute of Biodiversity
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Marker
attributes.value: rbcL.sh
attributes.tag: PrimerSeq_FWD_1
attributes.value: GCTATGCGCGAGCtgcCGAAATCGGTAGACGCTACG
attributes.tag: PrimerSeq_FWD_2
attributes.value: NA
attributes.tag: PrimerSeq_REV_1
attributes.value: GCGAGCTGCGCTAtgcCCATTGAGTCTCTGCACCTATC
attributes.tag: PrimerSeq_REV_2
attributes.value: NA
attributes.tag: SampleID_ASV
attributes.value: F-HEAD-6-R-TAIL-10
attributes.tag: SampleName
attributes.value: 099
attributes.tag: Submitter Id
attributes.value: F-HEAD-6-R-TAIL-10.rbcL_sh
attributes.tag: TagFWD
attributes.value: TGATACG
attributes.tag: TagREV
attributes.value: GTTAAGC
attributes.tag: adapters
attributes.value: FWD: TGATACG REV: GTTAAGC
attributes.tag: broad-scale environmental context
attributes.value: ENVO:01000245
attributes.tag: collection date
attributes.value: 2020-10-07
attributes.tag: environmental medium
attributes.value: ENVO:02000004
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: 51.47938909
attributes.tag: geographic location (longitude)
attributes.value: 12.15831301
attributes.tag: lat
attributes.value: 51.47938909076704
attributes.tag: local environmental context
attributes.value: nest tube of cavity-nesting hymenoptera
attributes.tag: lon
attributes.value: 12.158313007341283
attributes.tag: multiplex identifiers
attributes.value: FWD: TGATACG REV: GTTAAGC
attributes.tag: nucleic acid amplification
attributes.value: PCR
attributes.tag: nucleic acid extraction
attributes.value: Machery-Nagel NucleoSpin 96 Soil Kit
attributes.tag: pcr primers
attributes.value: FWD: GCTATGCGCGAGCtgcCGAAATCGGTAGACGCTACG REV: GCGAGCTGCGCTAtgcCCATTGAGTCTCTGCACCTATC
attributes.tag: project name
attributes.value: PilotNA
attributes.tag: removal_date
attributes.value: 2020-10-07
attributes.tag: sample name
attributes.value: F-HEAD-6-R-TAIL-10.rbcL_sh
attributes.tag: scientific_name
attributes.value: insect work metagenome
attributes.tag: site
attributes.value: Bageritz
attributes.tag: site_ID
attributes.value: BAG
attributes.tag: source material identifiers
attributes.value: 9_099_R2S3
attributes.tag: target gene
attributes.value: rbcL
attributes.tag: target subfragment
attributes.value: metabarcoding region
attributes.tag: trap_ID
attributes.value: 9_099_R2S3