title: Sample 10
organism: Mus musculus
description: Protocols: Samples were sorted into Trizol reagent, frozen at -80, and sent to Genewiz/Azenta Life Sciences for Ultra-low input RNA-seq. Total RNA was extracted from cells following the Trizol Reagent User Guide (Thermo Fisher Scientific). Extracted RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation.
attributes.tag: ENA first public
attributes.value: 2025-12-10
attributes.tag: INSDC center name
attributes.value: Salk
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16143:Sample 10
attributes.tag: age
attributes.value: 6-10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: Treg
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth condition
attributes.value: germ free
attributes.tag: individual
attributes.value: GF2
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: small intestine
attributes.tag: sample name
attributes.value: E-MTAB-16143:Sample 10
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6