title: Cer_Sis_deletion__BMpreB_Jk5_HTGTS_rep1
organism: Mus musculus
description: Protocols: Pre-B cells were isolated from 129SV WT or Cer/Sis-deleted mouse bone marrow by FACS sorting for B220+ CD43low IgM- cells Immature B cells were isolated from iPSC chimera-derived bone marrow by FACS sorting for B220+ CD19+ IgM- IgG1+ cells v-Abl cell lines were cultured in RPMI1640 media with 15% FBS RAG complementation was performed by infecting v-Abl cell lines with RAG2 retrovirus followed by selection with puromycin G1-arrest was performed by treating v-Abl cell lines with 3uM STI for 4 days DNA was lysed overnight at 56degC in a lysis buffer containing: 200mM NaCl, 0.2% SDS, 10mM Tris-HCl pH7.4, 2mM EDTA pH8.0, 200ug/ml proteinase K, precipitated by isopropanol and dissolved in buffer containing 10mM Tris-HCl pH7.4, 0.5mM EDTA pH8.0 HTGTS-V(D)J-seq libraries were prepared using linear-amplification PCR-mediated high-throughput genome-wide translocation sequencing protocol with indicated bait primers
attributes.tag: ENA first public
attributes.value: 2026-02-24
attributes.tag: INSDC center name
attributes.value: BCH
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16001_2:Cer_Sis_deletion__BMpreB_Jk5_HTGTS_rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: bone marrow pre-B cells
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Cer/Sis deletion
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-16001_2:Cer_Sis_deletion__BMpreB_Jk5_HTGTS_rep1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: 129SV