title: Sample 2
organism: Mus musculus
description: Protocols: Gastruloids were collected and washed with ice-cold PBS three times. They were incubated with TrypLE Express (Gibco, 12605028) for 2X 4 min in a heating block at 37oC, shaking at 300 rpm. After incubations, the gastruloids were dissociated into single cells by pipetting up and down 10 times. The cells were washed with PBS twice, centrifuged at 1300 rpm for 5 min at 4°C, and the supernatant was carefully discarded without disturbing the pellet. The cells were resuspended in PBS containing 0,04 % BSA (Sigma-Aldrich, A3059) and 1:5000 DAPI (Invitrogen, D1306) as a viability dye. The cell suspension was filtered through a FACS  (Falcon, 352235), and FACS analysis was performed (CytoFlex SRT Beckman Coulter). DAPI-negative and singlet populations were gated and collected. 10X Chromium Genomics workflow was performed by polyA enrichment (3'). 10X Chromium Genomics workflow was performed by polyA enrichment with single cell resolution.
attributes.tag: ENA first public
attributes.value: 2025-09-26
attributes.tag: INSDC center name
attributes.value: LUMC
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15522:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Rarb2-LacZ mES
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: culture stage
attributes.value: 120h
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: gastruloid
attributes.tag: sample name
attributes.value: E-MTAB-15522:Sample 2
attributes.tag: scientific_name
attributes.value: Mus musculus