title: Sample 9
organism: Mus musculus
description: Protocols: Cells were passaged every 2-3 days to maintain cultures between 10% and 90% confluency. For analysis, near-confluent cultures were released from culture vessels with trypsin, spun down, and snap-frozen in liquid nitrogen before further processing. E14tg2a mouse embryonic stem cells were grown in dishes pre-coated with 0.1 % gelatin (w/vol, Sigma) in GMEM supplemented with 10% fetal bovine serum, sodium pyruvate, 50 μM β-mercaptoethanol, glutamax, non-essential amino acids (all from Gibco/ThermoFisher) and 10 ng/ml murine leukemia inhibitory factor (LIF, Protein Expression Facility, MPI Dortmund). gDNA was isolated from E12tg2a mESCs using the Monarch Genomic DNA Purification Kit (NEB, T3010) according to the manufacturer's instructions. gDNA was sheared to an average fragment size of 200 bp using a Bioruptor Pico sonication device (Diagenode) Dyad Cap libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit. DNA purification and size selection was carried out using NEBNext Sample Purification Beads according to the protocol with bead volumes corresponding to a target fragment size of 200 bp. Fragment size distribution was verified using a TapeStation.
attributes.tag: ENA first public
attributes.value: 2026-04-14
attributes.tag: INSDC center name
attributes.value: Max Planck Institute of Molecular Physiology
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15857:Sample 9
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: ES-E14 cell
attributes.tag: cell type
attributes.value: mouse embryonic stem cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: mouse embryo stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: mecp2 variant
attributes.value: MECP2 HM
attributes.tag: sample name
attributes.value: E-MTAB-15857:Sample 9
attributes.tag: scientific_name
attributes.value: Mus musculus