title: Fleur11R3S20
organism: Arachis hypogaea
description: Protocols: Fresh pods were harvested at two time points: 20 days after flowering (DAF20) and 40 days after flowering (DAF40). These stages correspond to early and middle pod development, respectively, as reported in previous studies. For each genotype and at each developmental stage, pods were collected from three independent plants that exhibited similar size and appearance. The pods were rapidly pooled together and immediately frozen in liquid nitrogen for total RNA extraction. The four genotypes were grown at the Centre d'Étude Régional pour l'Amélioration de l'Adaptation à la Sécheresse (CERAAS) under screenhouse conditions. Total RNA was extracted using RNeasy Lipid Tissue Mini kit (Qiagen), following the manufacturer's procedure. RNA quality and quantity were assessed using a TapeStation 4200 System (Aligent Technologies). cDNA libraries were constructed for each sample using 1 µg of total RNA. Different index codes were added to each sample for identification. Briefly, cDNA libraries construction was carried out as following: first, mRNA was isolated using oligo-dT attached magnetic beads. Then, mRNA was fragmented, using divalent cations under elevated temperature (65°C for 5 min), before double strand cDNA synthesis. After these steps, adenylation and adaptor ligation were performed. Subsequently, an enrichment of cDNA fragments, consisting of PCR amplification, was carried out to constitute the libraries. Finally, two equimolar pools (pool 1: all samples at DAF20 and pool 2: all samples at DAF40) of cDNA libraries were formed according to the indexes used.
attributes.tag: ENA first public
attributes.value: 2025-09-26
attributes.tag: INSDC center name
attributes.value: CIRAD, UMR AGAP, Montpellier, France CIRAD, INRAE, AGAP, University Montpellier, Institut Agro, Montpellier, France
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15520:Fleur11R3S20
attributes.tag: age
attributes.value: 20
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: peanut
attributes.tag: cultivar
attributes.value: Fleur11
attributes.tag: developmental stage
attributes.value: early pod development
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth condition
attributes.value: Greenhouse
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: fresh pods
attributes.tag: sample name
attributes.value: E-MTAB-15520:Fleur11R3S20
attributes.tag: scientific_name
attributes.value: Arachis hypogaea