title: Sample 1
organism: Mycobacterium tuberculosis H37Rv
description: Protocols: 10 mL of the treated and control cultures were harvested by centrifugation at 3,000 x g for 5 min. 25 mL cultures were grown at 37°C without agitation to an optical density at 600 nm of 0.7.  Individual cultures at an optical density of 0.7 were treated with alpibectir 0.1 mg/L alone for 24 h or with alpibectir 0.1 mg/L for 20 h followed by ethionamide 2 mg/L for 4 h at 37°C without agitation. The pellets were resuspended in 1 mL of RNAproTM (FastRNA Pro Blue Kit, MP biomedicals) and homogenized in impact-resistant 2 mL tubes containing 0.1 mm silica spheres (Lysing Matrix B, MP biomedicals) using a FastPrep FP120 cell disrupter (Thermo Fisher Scientific) at 6.0 Hz for 40 s. The ribolysed cells were centrifuged at 12,000 x g to remove cellular debris and RNA was purified following the manufacturer's instructions. Ribosomal RNA (rRNA) depletion was performed using QIAseq FastSelect –5S/16S/23S Kits; Qiagen). Libraries for Illumina sequencing were prepared with the TruSeq RNA sample preparation kit version 2.0 rev. A (Illumina Inc.). All cDNA libraries were uniquely indexed.
attributes.tag: ENA first public
attributes.value: 2025-12-10
attributes.tag: INSDC center name
attributes.value: IPL_U1011
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16253:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: duration
attributes.value: 24
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth condition
attributes.value: 37°C without agitation
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-16253:Sample 1
attributes.tag: scientific_name
attributes.value: Mycobacterium tuberculosis
attributes.tag: strain
attributes.value: H37Rv
attributes.tag: type-material
attributes.value: type strain of Mycobacterium tuberculosis