title: Badetz 5_056_R2S3 rbcL metabarcoding
organism: insect work metagenome
description: Metabarcoding of rbcL from eDNA extracted from debris in nesting aids deployed on agricultural landscapes in Germany
attributes.tag: ENA first public
attributes.value: 2025-08-01
attributes.tag: INSDC center name
attributes.value: Thuenen-Institute of Biodiversity
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Marker
attributes.value: rbcL.sh
attributes.tag: PrimerSeq_FWD_1
attributes.value: GCTATGCGCGAGCtgcCGAAATCGGTAGACGCTACG
attributes.tag: PrimerSeq_FWD_2
attributes.value: NA
attributes.tag: PrimerSeq_REV_1
attributes.value: GCGAGCTGCGCTAtgcCCATTGAGTCTCTGCACCTATC
attributes.tag: PrimerSeq_REV_2
attributes.value: NA
attributes.tag: SampleID_ASV
attributes.value: F-HEAD-22-R-TAIL-4
attributes.tag: SampleName
attributes.value: 056
attributes.tag: Submitter Id
attributes.value: F-HEAD-22-R-TAIL-4.rbcL_sh
attributes.tag: TagFWD
attributes.value: ATAAAAT
attributes.tag: TagREV
attributes.value: CTGCGAG
attributes.tag: adapters
attributes.value: FWD: ATAAAAT REV: CTGCGAG
attributes.tag: broad-scale environmental context
attributes.value: ENVO:01000245
attributes.tag: collection date
attributes.value: 2020-10-06
attributes.tag: environmental medium
attributes.value: ENVO:02000004
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: 51.95379151
attributes.tag: geographic location (longitude)
attributes.value: 11.99047849
attributes.tag: lat
attributes.value: 51.95379151118672
attributes.tag: local environmental context
attributes.value: nest tube of cavity-nesting hymenoptera
attributes.tag: lon
attributes.value: 11.990478494990285
attributes.tag: multiplex identifiers
attributes.value: FWD: ATAAAAT REV: CTGCGAG
attributes.tag: nucleic acid amplification
attributes.value: PCR
attributes.tag: nucleic acid extraction
attributes.value: Machery-Nagel NucleoSpin 96 Soil Kit
attributes.tag: pcr primers
attributes.value: FWD: GCTATGCGCGAGCtgcCGAAATCGGTAGACGCTACG REV: GCGAGCTGCGCTAtgcCCATTGAGTCTCTGCACCTATC
attributes.tag: project name
attributes.value: PilotNA
attributes.tag: removal_date
attributes.value: 2020-10-06
attributes.tag: sample name
attributes.value: F-HEAD-22-R-TAIL-4.rbcL_sh
attributes.tag: scientific_name
attributes.value: insect work metagenome
attributes.tag: site
attributes.value: Badetz
attributes.tag: site_ID
attributes.value: BAD
attributes.tag: source material identifiers
attributes.value: 5_056_R2S3
attributes.tag: target gene
attributes.value: rbcL
attributes.tag: target subfragment
attributes.value: metabarcoding region
attributes.tag: trap_ID
attributes.value: 5_056_R2S3