title: CONTROL rbcL metabarcoding
organism: insect work metagenome
description: PCR negative control for metabarcoding of rbcL
attributes.tag: ENA first public
attributes.value: 2025-08-01
attributes.tag: INSDC center name
attributes.value: Thuenen-Institute of Biodiversity
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Marker
attributes.value: rbcL.sh
attributes.tag: PrimerSeq_FWD_1
attributes.value: GCTATGCGCGAGCtgcCGAAATCGGTAGACGCTACG
attributes.tag: PrimerSeq_FWD_2
attributes.value: NA
attributes.tag: PrimerSeq_REV_1
attributes.value: GCGAGCTGCGCTAtgcCCATTGAGTCTCTGCACCTATC
attributes.tag: PrimerSeq_REV_2
attributes.value: NA
attributes.tag: SampleID_ASV
attributes.value: F-HEAD-2-R-TAIL-8
attributes.tag: SampleName
attributes.value: CONTROL
attributes.tag: Submitter Id
attributes.value: F-HEAD-2-R-TAIL-8.rbcL_sh
attributes.tag: TagFWD
attributes.value: TGAGTCC
attributes.tag: TagREV
attributes.value: GTACATT
attributes.tag: adapters
attributes.value: FWD: TGAGTCC REV: GTACATT
attributes.tag: broad-scale environmental context
attributes.value: missing: control sample
attributes.tag: collection date
attributes.value: 2022-02-01
attributes.tag: environmental medium
attributes.value: missing: control sample
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: missing: control sample
attributes.tag: geographic location (longitude)
attributes.value: missing: control sample
attributes.tag: local environmental context
attributes.value: missing: control sample
attributes.tag: multiplex identifiers
attributes.value: FWD: TGAGTCC REV: GTACATT
attributes.tag: nucleic acid amplification
attributes.value: PCR
attributes.tag: nucleic acid extraction
attributes.value: Machery-Nagel NucleoSpin 96 Soil Kit
attributes.tag: pcr primers
attributes.value: FWD: GCTATGCGCGAGCtgcCGAAATCGGTAGACGCTACG REV: GCGAGCTGCGCTAtgcCCATTGAGTCTCTGCACCTATC
attributes.tag: project name
attributes.value: PilotNA
attributes.tag: sample name
attributes.value: F-HEAD-2-R-TAIL-8.rbcL_sh
attributes.tag: scientific_name
attributes.value: insect work metagenome
attributes.tag: source material identifiers
attributes.value: CONTROL
attributes.tag: target gene
attributes.value: rbcL
attributes.tag: target subfragment
attributes.value: metabarcoding region