title: OS_4
organism: Rattus norvegicus
description: Protocols: In this study, five femoral tissue samples were collected from the chronic osteomyelitis model group and the control group each, resulting in a total of ten samples. Total RNA extraction using Total RNA extraction Kit (DP431, TIANGEN, China). The quality and quantity of the purified RNA were determined by measuring the absorbance at 260nm/280nm(A260/A280) using Ultrafine spectrophotometer (N50 touch, IMPLEN, Germany). The integrity of RNA was further verified by 1.0% agarose gel electrophoresis For each sample, 500 ng of total RNA was used for cDNA synthesis via a strand-switching reverse transcription approach. The resulting cDNA was then amplified by PCR, followed by barcoding with the Native Barcode kit (SQK-NBD114.96) and purification using Ampure XP Beads. Finally, 300 fmol of the resulting library was loaded onto a PromethION flow cell (vR10.4.4) for sequencing.
attributes.tag: ENA first public
attributes.value: 2025-12-10
attributes.tag: INSDC center name
attributes.value: The Sixth Affiliated Hospital of Xinjiang Medical University
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16153:OS_4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: Norway rat
attributes.tag: disease
attributes.value: osteomyelitis
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: infect
attributes.value: Staphylococcus aureus
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: femoral tissue
attributes.tag: sample name
attributes.value: E-MTAB-16153:OS_4
attributes.tag: scientific_name
attributes.value: Rattus norvegicus