title: High-throughput RNA-Seq data of Thermus thermophilus HB27c cells expressing dlalcas12r-CRISPR locus
organism: Thermus thermophilus
description: Type V CRISPR-Cas locus derived from Lalka phages was cloned into pMK18 vector along with the CRISPR array containing spacers matching phiKo and phiFa bacteriophages. The lalcas12r gene contains mutations leading to inactivation of the RuvC domain. The plasmid was introduced into T. thermophilus HB27c cells. Small RNAs were isolated from the cell cultures and sequenced. The experiment was performed in two replicates.
attributes.tag: strain
attributes.value: HB27c
attributes.tag: isolation_source
attributes.value: laboratory
attributes.tag: collection_date
attributes.value: 2025-10-07
attributes.tag: geo_loc_name
attributes.value: Russia:Moscow
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental