title: pre_rearranged_Vk5_37_Jk1_Vk18_36_pro_del_Vk12_125_RSS_vAbl_iEk_3C_HTGTS_rep3
organism: Mus musculus
description: Protocols: Cells were fixed with 2% formaldehyde, quenched with glycine, lysed in a lysis buffer containing: 150mM NaCl, 0.5% NP-40, 1% Triton X-100, 50mM Tris-HCl pH7.5, 5mM EDTA pH8.0. Nuclei were digested with NlaIII, ligated with T4 DNA Ligase, treated with Proteinase K and RNase A prior to DNA precipitation. v-Abl cell lines were cultured in RPMI1640 media with 15% FBS G1-arrest was performed by treating v-Abl cell lines with 3uM STI for 4 days Cells were fixed with 2% formaldehyde, quenched with glycine, lysed in a lysis buffer containing: 150mM NaCl, 0.5% NP-40, 1% Triton X-100, 50mM Tris-HCl pH7.5, 5mM EDTA pH8.0. Nuclei were digested with NlaIII, ligated with T4 DNA Ligase, treated with Proteinase K and RNase A prior to DNA precipitation. 3C-HTGTS libraries were prepared using linear-amplification PCR-mediated high-throughput genome-wide translocation sequencing protocol with indicated bait primers
attributes.tag: ENA first public
attributes.value: 2026-02-24
attributes.tag: INSDC center name
attributes.value: BCH
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16007_3:pre_rearranged_Vk5_37_Jk1_Vk18_36_pro_del_Vk12_125_RSS_vAbl_iEk_3C_HTGTS_rep3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: v-Abl transformed pro-B cell line
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Rag2-/-, Emu-Bcl2, single Igk allele, pre-rearranged Vk5-37/Jk1_Vk18-36 pro del_Vk11-125 RSS
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sample name
attributes.value: E-MTAB-16007_3:pre_rearranged_Vk5_37_Jk1_Vk18_36_pro_del_Vk12_125_RSS_vAbl_iEk_3C_HTGTS_rep3
attributes.tag: scientific_name
attributes.value: Mus musculus