title: WT-EAU
organism: Mus musculus
description: ThThis sample consists of retinal single-cells isolated from the retinas of C57BL/6J (B6) mice with experimental autoimmune uveoretinitis (EAU) induced by human IRBP 1-20 (WT-EAU). The method for preparing the sample is described in the following reference: https://doi.org/10.3390/biomedicines13092049. Briefly, eight-week-old female B6 mice were subcutaneously immunized in the lumbar region with an emulsion of human IRBP 1-20 in PBS and CFA. To enhance the immune response, pertussis toxin was administered intraperitoneally. Three weeks after immunization, the mice were euthanized, and their retinas were enucleated. The retinas were then enzymatically digested with papain to obtain single-cell suspensions. Rod cells were subsequently removed using magnetic beads targeting CD74, and single-cell RNA sequencing was performed on the remaining retinal cells.
attributes.tag: sample_name
attributes.value: WT-EAU
attributes.tag: age
attributes.value: 11_weeks
attributes.tag: biomaterial_provider
attributes.value: Japan SLC
attributes.tag: breed
attributes.value: missing
attributes.tag: cell_type
attributes.value: retinal cells
attributes.tag: collection_date
attributes.value: 2021-12-21
attributes.tag: cultivar
attributes.value: missing
attributes.tag: dev_stage
attributes.value: missing
attributes.tag: disease
attributes.value: experimental autoimmune uveoretinitis
attributes.tag: ecotype
attributes.value: missing
attributes.tag: geo_loc_name
attributes.value: Japan
attributes.tag: isolate
attributes.value: missing
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL6J
attributes.tag: tissue
attributes.value: retina