title: Haloferax volcanii HTQ410 hypo salt - replicate 1
organism: Haloferax volcanii DS2
description: To investigate the effects of osmotic stress and c-di-AMP depletion on the transcriptome of Haloferax volcanii, we compared the transcriptional profiles of wild-type and c-di-AMP-reduced strains under both normal (2.5 M NaCl) and hypo-osmotic (1.8 M NaCl) conditions. Experiments were conducted using the H26 wild-type strain and the HTQ410 strain (c-di-AMP-reduced), in which the dacZ (HVO_1660) gene is placed under the control of a genetically engineered version of the tryptophan-inducible promoter (p.tnaA) of the tryptophanase gene tnaA (HVO_0789) (PMID: 30884174 ). Selected deletion strains were cultured overnight in YPC medium. Total RNA was extracted from H. volcanii strains H26 and HTQ410 cultivated to exponential phase in CAB medium using the RNeasy� Plus Mini Kit (Qiagen), following the manufacturer�s instructions.
attributes.tag: ENA first public
attributes.value: 2025-08-01
attributes.tag: INSDC center name
attributes.value: Microbiology_UR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: hvo_htq_nacl18_1
attributes.tag: collection date
attributes.value: 2020
attributes.tag: geographic location (country and/or sea)
attributes.value: Israel
attributes.tag: sample name
attributes.value: hvo_htq_nacl18_1
attributes.tag: scientific_name
attributes.value: Haloferax volcanii DS2
attributes.tag: strain
attributes.value: HTQ410