title: Mmat_Schier_st3_Apr10
organism: microbial mat metagenome
description: St3 Schiermoniekoog Microbial mat
attributes.tag: ENA first public
attributes.value: 2024-12-26
attributes.tag: External Id
attributes.value: SAMEA114321693
attributes.tag: Forward barcode
attributes.value: GTTCATTAAACT
attributes.tag: INSDC center name
attributes.value: Royal Netherlands Institute for Sea Research (NIOZ)
attributes.tag: INSDC last update
attributes.value: 2023-09-01T13:58:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Reverse barcode
attributes.value: GAGCGCCGAACA
attributes.tag: Submitter Id
attributes.value: NIOZ310.217.208
attributes.tag: barcodenames
attributes.value: 515F_Golay217 and 926RBC_Golay208
attributes.tag: collection date
attributes.value: 2010-04
attributes.tag: forwardprimersequence
attributes.value: GTGYCAGCMGCCGCGGTAA
attributes.tag: geographic location (country and/or sea)
attributes.value: Netherlands
attributes.tag: geographic location (latitude)
attributes.value: 29' 28.3626
attributes.tag: geographic location (longitude)
attributes.value: 6� 8' 20.9646
attributes.tag: nucleic acid extraction
attributes.value: Dneasy Powersoil kit
attributes.tag: pcr conditions
attributes.value: initial denaturation at 98 C for 30 s; 30 cycles of 98 C for 30 s, 50 C for 15 s, and 72 C for 30 s; final extension at 72 C for 5 minutes
attributes.tag: pcr primers
attributes.value: Forward Primer (515F) GTGYCAGCMGCCGCGGTAA Reverse Primer (926R) CCGYCAATTYMTTTRAGTTT
attributes.tag: project name
attributes.value: HglT primers
attributes.tag: purificationmethod
attributes.value: AmpureBeads
attributes.tag: reverseprimersequence
attributes.value: CCGYCAATTYMTTTRAGTTT
attributes.tag: sample name
attributes.value: NIOZ310.217.208
attributes.tag: scientific_name
attributes.value: microbial mat metagenome
attributes.tag: sequencing method
attributes.value: Illumina MiSeq
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V4V5
attributes.tag: targetedampliconsize
attributes.value: 600bp400bp