organism: root metagenome
attributes.tag: host
attributes.value: Zea mays
attributes.tag: isolation_source
attributes.value: root
attributes.tag: collection_date
attributes.value: not collected
attributes.tag: geo_loc_name
attributes.value: USA:North Dakota
attributes.tag: lat_lon
attributes.value: not collected
attributes.tag: library_id
attributes.value: Plate8_peasaltinity
attributes.tag: title
attributes.value: Amplicon_culturomics from field-grown crop plants
attributes.tag: library_strategy
attributes.value: AMPLICON
attributes.tag: library_source
attributes.value: METAGENOMIC
attributes.tag: library_selection
attributes.value: PCR
attributes.tag: library_layout
attributes.value: paired
attributes.tag: platform
attributes.value: ILLUMINA
attributes.tag: instrument_model
attributes.value: Illumina MiSeq
attributes.tag: design_description
attributes.value: Amplified the V4 region of the 16S rRNA gene using the V4_515F (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGTGCCAGCMGCCGCGGTAA -3) and V4_R (5- GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC -3) primers of DNA extracted from barley spikes to compareautomated and manual library preparation methods.
attributes.tag: filetype
attributes.value: fastq
attributes.tag: filename
attributes.value: Plate8_peasaltinity_R1_001.fastq.gz
attributes.tag: filename2
attributes.value: Plate8_peasaltinity_R2_001.fastq.gz
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental