title: Arabidopsis leaves infected with Sclerotinia 2
organism: Arabidopsis thaliana
attributes.tag: ENA first public
attributes.value: 2025-11-24
attributes.tag: INSDC center name
attributes.value: LIPME
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Ath_FRT_231018
attributes.tag: age
attributes.value: 5W
attributes.tag: collection date
attributes.value: 2023-10-18
attributes.tag: common name
attributes.value: thale cress
attributes.tag: dev_stage
attributes.value: 5 week old plants
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: experimental factor 1
attributes.value: RNA
attributes.tag: experimental factor 2
attributes.value: Infected
attributes.tag: genotype
attributes.value: Col-0
attributes.tag: geographic location (country and/or sea)
attributes.value: France
attributes.tag: growth condition
attributes.value: Grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s
attributes.tag: infect
attributes.value: Sclerotinia sclerotiorum
attributes.tag: organism part
attributes.value: leaf
attributes.tag: phenotype
attributes.value: Susceptible
attributes.tag: protocol
attributes.value: Arabidopsis thaliana Columbia-0 genotype was grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s. Leaves of five-week-old plants were inoculated with S. sclerotiorum strain 1980 (ATCC18683) using 0.5-cm-wide plugs of Potato Dextrose Agar (PDA) medium colonized by the fungus. Control samples were also prepared identially but with uncolonised agar plugs. The plugs were placed in the center of the adaxial surface of leaves, and plants were incubated in trays sealed with plastic wrap to maintain 80% relative humidity. Entire leaves were collected individually for nuclei extraction 24 hours, with two independent biological replicates per treatment. One complete leaf per sample was collected and agar plugs were removed. . Leaves were transferred to pre-chilled lysis buffer and chopped with a razor blade, as described in Guillotin et al., 2023 (https://doi.org/10.1038/s41586-023-06053-0). Immediately after 10µm filtration at 1000g centrifugation, the nuclei were stained with propidium iodide and isolated using fluorescense-activated cell sorting (FACS) performed by the Cytek Aurora Cell Sorter form Cytometry TRI Genotoul facility (Toulouse, France). FACS isolated 6,000 nuclei per sample. SnRNA-seq libraries were prepared at the Get-Santé facility (INSERM Rangueil, France) using the Chromium Next GEM Single Cell 3\' Kit v3.1 (10x Genomics reference) and the Chromium Next GEM Chip G Single Cell Kit (10x Genomics), following the manufacturer\'s protocol.
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: Ath_FRT_231018
attributes.tag: sampling time point
attributes.value: 24H
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana
attributes.tag: time
attributes.value: 24H