title: Arabidopsis leaves treated with audio 1000Hz
organism: Arabidopsis thaliana
attributes.tag: ENA first public
attributes.value: 2025-11-24
attributes.tag: INSDC center name
attributes.value: LIPME
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Ade_Atha_AW1000
attributes.tag: age
attributes.value: 5W
attributes.tag: collection date
attributes.value: 2023-10-18
attributes.tag: common name
attributes.value: thale cress
attributes.tag: dev_stage
attributes.value: 5 week old plants
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: experimental factor 1
attributes.value: RNA
attributes.tag: experimental factor 2
attributes.value: Sine wave 1000 Hz
attributes.tag: genotype
attributes.value: Col-0
attributes.tag: geographic location (country and/or sea)
attributes.value: France
attributes.tag: growth condition
attributes.value: Grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s
attributes.tag: organism part
attributes.value: leaf
attributes.tag: protocol
attributes.value: Arabidopsis thaliana Columbia-0 genotype was grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s. Five week old plants were exposed to an audio sine wave at 1000 Hz for 15 minutes, and leaves were excised immediately after treatment for processing. Leaves were transferred to pre-chilled lysis buffer and chopped with a razor blade, as described in Guillotin et al., 2023 (https://doi.org/10.1038/s41586-023-06053-0). Immediately after 10µm filtration at 1000g centrifugation, the nuclei were stained with propidium iodide and isolated using fluorescense-activated cell sorting (FACS) performed by the Cytek Aurora Cell Sorter form Cytometry TRI Genotoul facility (Toulouse, France). FACS isolated 6,000 nuclei per sample. SnRNA-seq libraries were prepared at the Get-Santé facility (INSERM Rangueil, France) using the Chromium Next GEM Single Cell 3\' Kit v3.1 (10x Genomics reference) and the Chromium Next GEM Chip G Single Cell Kit (10x Genomics), following the manufacturer\'s protocol.
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: Ade_Atha_AW1000
attributes.tag: sampling time point
attributes.value: 24H
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana