title: Individual plants/genotypes were grown in a greenhouse for 2 weeks using propagation trays. Leaf segments were placed in 2 ml tubes (in cool ice) and stored at -80°C. The leaf samples were then lyophilized at -45°C to -50°C for 3 days. 20mg of lyophilized sample was homogenized using the GenoGrinder. DNA was extracted using the CTAB protocol. After the precipitation with isopropanol, the collected DNA was washed 2 times with ethanol and then dried at room temperature overnight inside a fume hood.
organism: Hordeum vulgare var. distichon
attributes.tag: biological material ID
attributes.value: AGENT:AB_04178
attributes.tag: biological material ID
attributes.value: GAT_LIMS:2638843
attributes.tag: biological material ID
attributes.value: LBN002:LRC_SSD_IG_35721
attributes.tag: collection date
attributes.value: not collected
attributes.tag: biological material ploidy
attributes.value: diploid
attributes.tag: collected by
attributes.value: not collected
attributes.tag: material source geographic location
attributes.value: not collected
attributes.tag: biological material sowing date
attributes.value: not collected
attributes.tag: record time IPK LIMS
attributes.value: 2021-09-03
attributes.tag: material source altitude
attributes.value: not collected
attributes.tag: material source description
attributes.value: not collected
attributes.tag: biological material altitude
attributes.value: not collected
attributes.tag: biological material geographic location
attributes.value: Lebanon
attributes.tag: plant anatomical entity
attributes.value: leaf
attributes.tag: material source longitude
attributes.value: not collected
attributes.tag: organism
attributes.value: Hordeum vulgare var. distichon
attributes.tag: biological material coordinates uncertainty
attributes.value: not collected
attributes.tag: material source ID
attributes.value: LBN002:IG 35721
attributes.tag: biological material longitude
attributes.value: not collected
attributes.tag: checklist
attributes.value: BSDC00002
attributes.tag: infraspecific name
attributes.value: Hordeum distichon
attributes.tag: sample description
attributes.value: Individual plants/genotypes were grown in a greenhouse for 2 weeks using propagation trays. Leaf segments were placed in 2 ml tubes (in cool ice) and stored at -80°C. The leaf samples were then lyophilized at -45°C to -50°C for 3 days. 20mg of lyophilized sample was homogenized using the GenoGrinder. DNA was extracted using the CTAB protocol. After the precipitation with isopropanol, the collected DNA was washed 2 times with ethanol and then dried at room temperature overnight inside a fume hood.
attributes.tag: biological material latitude
attributes.value: not collected
attributes.tag: biological material preprocessing
attributes.value: This is a single seed descent (SSD) derived during 2021 from the accession number IG 35721 maintained by International Centre for Agricultural Research in Dry Areas, Beirut, Lebanon
attributes.tag: material source coordinates uncertainty
attributes.value: not collected
attributes.tag: project name
attributes.value: AGENT - Activated Genebank Network
attributes.tag: genus
attributes.value: Hordeum
attributes.tag: species
attributes.value: distichon
attributes.tag: material source latitude
attributes.value: not collected
attributes.tag: variety
attributes.value: distichon