title: 40092S00
organism: human gut metagenome
attributes.tag: ENA first public
attributes.value: 2025-11-05
attributes.tag: INSDC center name
attributes.value: MV
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: ena-SAMPLE-TAB-12-06-2025-10:00:56:946-45411
attributes.tag: adapters
attributes.value: fwd: AATGATACGGCGACCACCGAGATCTACACGCTTATGGTAATTGT, rev: CAAGCAGAAGACGGCATACGAGATAGTCAGCCAGCC
attributes.tag: broad-scale environmental context
attributes.value: city [ENVO:00000856]; human settlement [ENVO:01001829]
attributes.tag: collection date
attributes.value: 2023-09-27
attributes.tag: environmental medium
attributes.value: feces [UBERON:0001988]
attributes.tag: geographic location (country and/or sea)
attributes.value: Brazil
attributes.tag: geographic location (latitude)
attributes.value: -23
attributes.tag: geographic location (longitude)
attributes.value: -46
attributes.tag: geographic location (region and locality)
attributes.value: Sao Paulo
attributes.tag: host body product
attributes.value: Feces [FMA:64183]; feces [UBERON:0001988]
attributes.tag: host subject id
attributes.value: UASP40092
attributes.tag: license information
attributes.value: CC-BY-NC license issued by Christian_Hoffmann
attributes.tag: local environmental context
attributes.value: human body [FMA:20394]; feces [UBERON:0001988]
attributes.tag: multiplex identifiers
attributes.value: AATGATACGGCGACCACCGAGATCTACACGCT
attributes.tag: nucleic acid amplification
attributes.value: https://dx.doi.org/10.17504/protocols.io.kqdg3dzzl25z/v2
attributes.tag: nucleic acid extraction
attributes.value: Maxwell RSC PureFood GMO and Authentication Kit AS1600
attributes.tag: pcr conditions
attributes.value: initial denaturation:94degC_3min; denaturation:94degC_45s; annealing:50degC_60s; extension:72degC_90s; final extension:72degC_10min;
attributes.tag: pcr primers
attributes.value: fwd: GTGYCAGCMGCCGCGGTAA, rev: GGACTACNVGGGTWTCTAAT
attributes.tag: project name
attributes.value: Urban Agriculture Sao Paulo
attributes.tag: sample biobank
attributes.value: The Microbiota Vault
attributes.tag: sample coordinator
attributes.value: Christian_Hoffmann
attributes.tag: sample coordinator ORCID
attributes.value: 0000-0003-1736-8007
attributes.tag: sample coordinator affiliation
attributes.value: USP, School Of Pharmaceutical Sciences
attributes.tag: sample name
attributes.value: ena-SAMPLE-TAB-12-06-2025-10:00:56:946-45411
attributes.tag: sample shipping temperature
attributes.value: -80
attributes.tag: sample storage duration
attributes.value: P2M18D
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: sample transport temperature
attributes.value: 4
attributes.tag: scientific_name
attributes.value: human gut metagenome
attributes.tag: sequencing_run
attributes.value: run_404
attributes.tag: target gene
attributes.value: 16S
attributes.tag: target subfragment
attributes.value: V4