title: Arabidopsis leaves treated with wind
organism: Arabidopsis thaliana
attributes.tag: ENA first public
attributes.value: 2025-11-24
attributes.tag: INSDC center name
attributes.value: LIPME
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Ade_Atha_V1_SI
attributes.tag: age
attributes.value: 5W
attributes.tag: collection date
attributes.value: 2023-10-18
attributes.tag: common name
attributes.value: thale cress
attributes.tag: dev_stage
attributes.value: 5 week old plants
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: experimental factor 1
attributes.value: RNA
attributes.tag: experimental factor 2
attributes.value: Wind 1.2 m/s
attributes.tag: genotype
attributes.value: Col-0
attributes.tag: geographic location (country and/or sea)
attributes.value: France
attributes.tag: growth condition
attributes.value: Grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s
attributes.tag: organism part
attributes.value: leaf
attributes.tag: protocol
attributes.value: Arabidopsis thaliana Columbia-0 genotype was grown in Jiffy pots under controlled conditions at 22°C with a 9-hour light period at an intensity of 120 μmol/m2/s. Five week old plants were exposed to wind from a fan at 1.2 m/s for 15 minutes, and leaves were excised immediately after treatment for processing. Leaves were transferred to pre-chilled lysis buffer and chopped with a razor blade, as described in Guillotin et al., 2023 (https://doi.org/10.1038/s41586-023-06053-0). Immediately after 10µm filtration at 1000g centrifugation, the nuclei were stained with propidium iodide and isolated using fluorescense-activated cell sorting (FACS) performed by the Cytek Aurora Cell Sorter form Cytometry TRI Genotoul facility (Toulouse, France). FACS isolated 6,000 nuclei per sample. SnRNA-seq libraries were prepared at the Get-Santé facility (INSERM Rangueil, France) using the Chromium Next GEM Single Cell 3\' Kit v3.1 (10x Genomics reference) and the Chromium Next GEM Chip G Single Cell Kit (10x Genomics), following the manufacturer\'s protocol.
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: Ade_Atha_V1_SI
attributes.tag: sampling time point
attributes.value: 24H
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana