title: Individual plants were grown in a nethouse. Leaf segments (each approximately 5 cm in length and 0.5 to 0.8 cm in width) were collected before 11:00 AM and set in 96-well polypropylene plate containing two stainless steel beads (1X3 mm + 1X5mm). The leaf were samples dried using lyophilizer and grinded. DNA extraction was performed using CTAB DNA Extraction Protocol (double chloroform). Each sample was checked for quantity and quality using PicoGreen, nanodrop and gel-elctrophoresis, respectively.
organism: Hordeum vulgare subsp. spontaneum
attributes.tag: biological material ID
attributes.value: GAT_LIMS:2679688
attributes.tag: biological material ID
attributes.value: ISR003:HSEU-1807-2
attributes.tag: biological material ID
attributes.value: AGENT:AB_03750
attributes.tag: collection date
attributes.value: not collected
attributes.tag: biological material ploidy
attributes.value: diploid
attributes.tag: collected by
attributes.value: not collected
attributes.tag: material source geographic location
attributes.value: Israel
attributes.tag: biological material sowing date
attributes.value: not collected
attributes.tag: record time IPK LIMS
attributes.value: 2021-09-29
attributes.tag: material source altitude
attributes.value: not collected
attributes.tag: material source description
attributes.value: not collected
attributes.tag: biological material altitude
attributes.value: not collected
attributes.tag: biological material geographic location
attributes.value: Israel
attributes.tag: plant anatomical entity
attributes.value: leaf
attributes.tag: material source longitude
attributes.value: not collected
attributes.tag: organism
attributes.value: Hordeum vulgare subsp. spontaneum
attributes.tag: biological material coordinates uncertainty
attributes.value: not collected
attributes.tag: material source ID
attributes.value: ISR003:HS-1807-2
attributes.tag: biological material longitude
attributes.value: not collected
attributes.tag: checklist
attributes.value: BSDC00002
attributes.tag: infraspecific name
attributes.value: Hordeum spontaneum
attributes.tag: sample description
attributes.value: Individual plants were grown in a nethouse. Leaf segments (each approximately 5 cm in length and 0.5 to 0.8 cm in width) were collected before 11:00 AM and set in 96-well polypropylene plate containing two stainless steel beads (1X3 mm + 1X5mm). The leaf were samples dried using lyophilizer and grinded. DNA extraction was performed using CTAB DNA Extraction Protocol (double chloroform). Each sample was checked for quantity and quality using PicoGreen, nanodrop and gel-elctrophoresis, respectively.
attributes.tag: biological material latitude
attributes.value: not collected
attributes.tag: biological material preprocessing
attributes.value: This is a single seed descent (SSD) derived during 2021 from the accession number HS-1807-2 maintained by Lieberman Germplasm Bank, Institute for Cereal Crops Improvement, Tel-Aviv University, Tel-Aviv, Israel
attributes.tag: material source coordinates uncertainty
attributes.value: not collected
attributes.tag: project name
attributes.value: AGENT - Activated Genebank Network
attributes.tag: genus
attributes.value: Hordeum
attributes.tag: species
attributes.value: spontaneum
attributes.tag: material source latitude
attributes.value: not collected
attributes.tag: sub_species
attributes.value: spontaneum