organism: Homo sapiens
description: Protocols: Pateint-derived iPSC with FUS(R244RR) and isogenic line with NGN2, ISL1, and LHX3 cassettes were differentiated into induced motor neurons (iMNs) using established protocols (Fernandopulle et al., 2018, doi: 10.1002/cpcb.51). Cells were grown on filter membrane inserts (pore size 1.0 μm PET membrane for 6-well plates Millicell PLRP06H48, Millipore) to isolate axonal and somatodendritic compartments. Whole iMNs and subcellular compartments were collected on day 21 of the differentiation protocol. RNA was extracted from using TRIfast (PeqLab 30-2010). For a detailed description of harvesting material from filter membranes see our prior work (Ludwik et al., 2019, doi: 10.1016/j.ymeth.2019.02.002).  100 ng of total RNA obtained from either total cells or separated compartments was used for library preparation with the Truseq stranded mRNA library prep kit (Illumina 20020595) according to the manufacturer's recommendation.
attributes.tag: ENA first public
attributes.value: 2025-08-05
attributes.tag: INSDC center name
attributes.value: Berlin Institute for Medical Systems Biology
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14354:iMN_FUSR244RR_soma_3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: hiPSC
attributes.tag: cell_type
attributes.value: induced motor neuron
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: differentiation
attributes.value: motor neuron differential protocol
attributes.tag: disease
attributes.value: ALS
attributes.tag: genotype
attributes.value: FUSR244RR
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: soma
attributes.tag: progenitor cell type
attributes.value: pluripotent stem cell
attributes.tag: sample name
attributes.value: E-MTAB-14354:iMN_FUSR244RR_soma_3
attributes.tag: scientific_name
attributes.value: Homo sapiens