organism: Homo sapiens
description: Protocols: For PBMC: Standard venopuncture followed by ficoll extraction of PBMC For BM:  For HDF:Human dermal fibroblast (HDF) isolation of the proband was performed within three days of the skin biopsies. The biopsy was transferred to a sterile petri dish. Dermis and hypodermis were removed and discarded. Epidermis was cut into small pieces and placed in 25 cm2 flasks. Culture media (Dulbecco's Modified Eagle Medium (10566016, Gibco™, Thermo Fisher Scientific, Waltham, MA, USA) (DMEM) supplemented with 10% inactivated foetal bovine serum (FBS), 40 U/ml penicillin, and 50 mg/ml streptomycin was added to cover the surface after 15 minutes. The media was changed after 24 hours. The cells were passed at 80% confluency using trypsinization. Control HDFs were purchased from Promocell (Heidelberg, Germany). RNA was extracted using the RNeasy Mini Kit (74106, Qiagen, Hilden, Germany).  SMARTer® Stranded Total RNA-Seq Kit v2 (Takara, Otsu, Shiga, Japan)
attributes.tag: ENA first public
attributes.value: 2025-08-26
attributes.tag: INSDC center name
attributes.value: Laboratoire d'ImmunoRhumatologie Moléculaire, plateforme GENOMAX, INSERM UMR_S 1109
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15443:39719_1_HDF
attributes.tag: age
attributes.value: neonate
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: neonate
attributes.tag: disease
attributes.value: normal
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: Control3
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: human dermal fibroblast
attributes.tag: sample name
attributes.value: E-MTAB-15443:39719_1_HDF
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male