organism: invertebrate metagenome
description: This sample represents the bacterial community associated with the egg strings of the marine opisthobranch Aplysia depilans, collected from the northern coast of Portugal (Molhe Beach, Porto). Egg strings are gelatinous structures that encase developing embryos and provide physical and biochemical protection against environmental stressors and microbial colonization in the intertidal zone. The sample was used for 16S rRNA gene amplicon sequencing on the Ion Torrent S5 platform (Thermo Fisher Scientific) using the Ion 16S Metagenomics Kit, which targets six hypervariable regions (V2, V3, V4, V6-7, V8, and V9) of the bacterial 16S rRNA gene. DNA was extracted directly from the egg string material and processed according to the manufacturer's protocol. Taxonomic classification of the resulting reads was performed using the Ion Reporter 16S Metagenomics workflow, applying a two-step BLAST alignment against the MicroSEQ ID bacterial reference library and the Greengenes database, following Clinical and Laboratory Standards Institute (CLSI) thresholds for identity (less than 97 percent for family level, greater than 97 percent for genus level, and greater than 99 percent for species level). This dataset contributes to the characterization of the microbiome associated with Aplysia depilans egg strings, providing data useful for studies on host-microbe interactions and microbial ecology in coastal marine environments.
attributes.tag: collection_date
attributes.value: 2025-08/2025-09
attributes.tag: env_broad_scale
attributes.value: marine biome [ENVO:00000447]
attributes.tag: env_local_scale
attributes.value: intertidal zone [ENVO:00000316]
attributes.tag: env_medium
attributes.value: seawater [ENVO:00002149]
attributes.tag: geo_loc_name
attributes.value: Portugal: Molhe beach, Porto
attributes.tag: host
attributes.value: Aplysia depilans (marine opisthobranch)
attributes.tag: lat_lon
attributes.value: 41.1580 N 8.6844 W
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated