organism: mouse gut metagenome
description: mouse 143, week 08
attributes.tag: ENA first public
attributes.value: 2025-08-26
attributes.tag: INSDC center name
attributes.value: Max Rubner-Institute
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: ena-SAMPLE-TAB-14-08-2025-09:35:37:517-75846
attributes.tag: adapters
attributes.value: fwd:ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNCA::rev:CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
attributes.tag: broad-scale environmental context
attributes.value: ENVO_01001033:digestive tract environment
attributes.tag: collection date
attributes.value: 2016-11-22
attributes.tag: diet group
attributes.value: CE
attributes.tag: environmental medium
attributes.value: ENVO_00002003:fecal material
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: 49.0139822
attributes.tag: geographic location (longitude)
attributes.value: 8.4240071
attributes.tag: host age
attributes.value: 18.000
attributes.tag: host body product
attributes.value: fma64183:Feces
attributes.tag: host common name
attributes.value: Mouse
attributes.tag: host diet
attributes.value: per kg: 200 g casein, 395 g corn starch, 50 g sucrose, 190 g lard, 10 g vitamin mix, 50 g mineral mix, 3 g L-cysteine, 3 g choline chloride, 100 g cellulose
attributes.tag: host growth conditions
attributes.value: single housed in individually ventilated cages
attributes.tag: host scientific name
attributes.value: Mus musculus
attributes.tag: host sex
attributes.value: male
attributes.tag: host subject id
attributes.value: 143
attributes.tag: host subspecific genetic lineage
attributes.value: C57BL/6
attributes.tag: host taxid
attributes.value: 10090
attributes.tag: local environmental context
attributes.value: ENVO_01001029:fecal environment
attributes.tag: multiplex identifiers
attributes.value: R1:GCATCC_R2:ATTGGC
attributes.tag: nucleic acid amplification
attributes.value: doi:10.3390/nu11102348
attributes.tag: nucleic acid extraction
attributes.value: doi:10.3390/nu11102348
attributes.tag: pcr conditions
attributes.value: 3 PCR steps: enrichment PCR - 10 cycles, custom barcode integration - 15 cycles, illumina index integration - 20 cycles, see doi:10.3390/nu11102348 for detailed protocol
attributes.tag: pcr primers
attributes.value: fwd:AGAGTTTGATCMTGGCTCAG::rev:TGCTGCCTCCCGTAGGAGT
attributes.tag: project name
attributes.value: Whole Grain Mouse Gut Microbiome
attributes.tag: sample name
attributes.value: ena-SAMPLE-TAB-14-08-2025-09:35:37:517-75846
attributes.tag: scientific_name
attributes.value: mouse gut metagenome
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V1V2
attributes.tag: week-after-start
attributes.value: 8