organism: metagenome
description: This study investigates the nitrogen removal potential of a synthetic microbial community (SynCom) derived from marine plastic biofilms (the "plastisphere"). Three bacterial strains (Pseudomonas stutzeri P5F4-3, Shimia sp. pe10, and Ruegeria sp. PMMA7) were isolated from this environment and assembled into a defined SynCom. The community was cultured in Marine Broth 2216E with carbon sources supplied at 10% of the standard concentration, under nitrate stress (20 mg/L NO3-N). 16S rRNA gene (V3-V4 region) amplicon sequencing was performed. This BioSample represents the SynCom cultured at 7% salinity for 24 hours (biological replicate 3).
attributes.tag: collection_date
attributes.value: 2025-03-01
attributes.tag: depth
attributes.value: 1 m
attributes.tag: elev
attributes.value: -1
attributes.tag: env_broad_scale
attributes.value: Marine coastal water
attributes.tag: env_local_scale
attributes.value: Anthropogenic marine biofilm on plastic surface
attributes.tag: env_medium
attributes.value: Marine Broth 2216E
attributes.tag: geo_loc_name
attributes.value: China: Qingdao, Shandong
attributes.tag: lat_lon
attributes.value: 36.07 N 120.38 E
attributes.tag: biomass
attributes.value: OD=0.8
attributes.tag: chlorophyll
attributes.value: not applicable
attributes.tag: collection_method
attributes.value: Plastic sheets deployed in nylon mesh bags; biofilm removed by sonication and vortexing
attributes.tag: isolation_source
attributes.value: Biofilm on submerged polyethylene plastic sheet
attributes.tag: nitrate
attributes.value: 20 mg/L
attributes.tag: nitrite
attributes.value: 0
attributes.tag: oxy_stat_samp
attributes.value: aerobic
attributes.tag: perturbation
attributes.value: High-salinity (7%) and nitrate amendment (20 mg/L NO3-N) stress treatment
attributes.tag: ph
attributes.value: 8.5
attributes.tag: phosphate
attributes.value: Disodium Phosphate（磷酸二钠） 0.008g
attributes.tag: pressure
attributes.value: 1 atm
attributes.tag: salinity
attributes.value: 7%
attributes.tag: samp_collect_device
attributes.value: Nylon mesh bag
attributes.tag: samp_mat_process
attributes.value: Cells were harvested by centrifugation and snap-frozen in liquid nitrogen
attributes.tag: samp_store_temp
attributes.value: -80 degree Celsius
attributes.tag: samp_vol_we_dna_ext
attributes.value: 1 g
attributes.tag: size_frac
attributes.value: >0.22 um
attributes.tag: source_material_id
attributes.value: Dnitrification-syn7%-24h-3
attributes.tag: temp
attributes.value: 25
attributes.tag: turbidity
attributes.value: 0.8
attributes.tag: water_content
attributes.value: 1
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.microbial