organism: Homo sapiens
description: Protocols: HT-29 control and DZIP3 KD cells were collected from 3 different wells for each group in a 6 well plate RNA was extracted from HT-29 cells(three biological replicates) using RNeasy mini kit (QIAGEN, #74104). The quality and quantity of total RNA was checked using agarose gel and Qubit 4.0. After assessing the quality of RNA, 800-900 ng of total RNA was subjected to NEBNext® ultra™ directional RNA library prep kit for Illumina® (#E7420) using NEBNextPoly(A) mRNA magnetic isolation module (NEB #E7490). The QC of the prepared library was performed using High-Sensitivity Tape Station Kit (Agilent 2200, 5067-5585 (reagents) #5067-5584 ( screen tapes)) for fragment length distribution and Qubit dsDNA HS assay kit (Invitrogen, Q32851) for quantifying the library.
attributes.tag: ENA first public
attributes.value: 2025-11-08
attributes.tag: INSDC center name
attributes.value: CSIR-Centre for Cellular & Molecular Biology
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14608:SCD3HT29_SCR2_S8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: HT-29 Control shRNA
attributes.tag: cell_type
attributes.value: epithelial cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: Adenocarcinoma; Colorectal
attributes.tag: genotype
attributes.value: Control shRNA
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-14608:SCD3HT29_SCR2_S8
attributes.tag: scientific_name
attributes.value: Homo sapiens