organism: Streptococcus sp. KHUD_013
attributes.tag: strain
attributes.value: KHUD_013
attributes.tag: collection_date
attributes.value: 2023-11-01
attributes.tag: env_broad_scale
attributes.value: human-associated habitat
attributes.tag: env_local_scale
attributes.value: oral cavity
attributes.tag: env_medium
attributes.value: Fastidious anaerobe agar (MB cell (cat. MB-F2169)), 0.25% mucin from porcine stomach (sigma cat. M2378), 10% Fetal bovine serum (Gibco cat. 16000044), 5% sheep blood
attributes.tag: geo_loc_name
attributes.value: South Korea: Seoul
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: isol_growth_condt
attributes.value: Bacterial samples were collected by scraping the buccal mucosa using sterile cotton swabs. The swabs were then soaked in phosphate-buffered saline (PBS) to prepare a bacterial suspension, which was serially diluted. A 200 microL aliquot of the diluted suspension was plated onto Fastidious anaerobe agar supplemented with 5% sheep blood, 0.25% mucin, and 10% fetal bovine serum. The plates were incubated anaerobically at 37 C for 24 hours in an anaerobic chamber containing 10% CO?.., 10% H?.., and a nitrogen-balanced atmosphere.
attributes.tag: lat_lon
attributes.value: 37.59 N 127.05 E
attributes.tag: num_replicons
attributes.value: NA
attributes.tag: ref_biomaterial
attributes.value: NA
attributes.tag: BioSampleModel
attributes.value: MIGS.ba
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.human-oral