organism: Mus musculus
description: Protocols: The distal region of a limb bud was isolated from Sox9-EGFP mouse embryos. Ectoderm was removed. The isolated mesenchyme cells were further dissociated by pipetting into a single-cell suspension and sorted with FACS Aria (BD) for the Sox9-EGFP signal-positive or signal-negative populations. Total RNA was extracted from each of the sorted populations. cDNA library were prepared from 10 ng of total RNA with SMARTer Ultra Low Input RNA Kit for Sequencing v3 (Clontech) and NEBNext Ultra DNA Library Prep Kit for Illumina according to the manufacturer's protocols.
attributes.tag: ENA first public
attributes.value: 2025-05-09
attributes.tag: INSDC center name
attributes.value: EMBL
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14105:Q_RHL4_GFP_pos_8636
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: limb bud mesenchyme cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: mE11:10
attributes.tag: genotype
attributes.value: Sox9-positive
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: Embryo 4
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: Hindlimb autopod
attributes.tag: sample name
attributes.value: E-MTAB-14105:Q_RHL4_GFP_pos_8636
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: Sox9-EGFP