organism: Mus musculus
description: Primary mouse hepatocytes were treated with IL22 for 2 hours. For ChIP-seq, 2  10^7 cells were cross-linked with 1% formaldehyde for 20 min at 4C and quenched with glycine. Nuclear extracts were prepared and chromatin was fragmented using sonication. Cross-links were reversed, and samples were treated with RNase and proteinase K. DNA was purified, quantified, and used for library preparation.
attributes.tag: breed
attributes.value: C57BL/6J
attributes.tag: age
attributes.value: 8 weeks
attributes.tag: dev_stage
attributes.value: adult
attributes.tag: collection_date
attributes.value: 2025
attributes.tag: geo_loc_name
attributes.value: China: Shanghai
attributes.tag: sex
attributes.value: missing
attributes.tag: tissue
attributes.value: liver
attributes.tag: cell_type
attributes.value: hepatocyte
attributes.tag: treatment
attributes.value: input
attributes.tag: BioSampleModel
attributes.value: Model organism or animal