organism: Triticum aestivum
description: Protocols: Cereal cyst nematode H. avenae (Treub) Chitwood were collected from an infested wheat field in Qingdao, China. The roots of Wild type and overexpressed wheat were inoculated with Ha nematodes, and the roots were collected 3 days later, with no inoculation as a negative control. The wheat cultivar Fielder was used for transgenic studies, plant-nematode interaction analyses, and parasitism assays.Both plant species were maintained in growth chambers under controlled conditions (23°C, 16-h light/8-h dark photoperiod). First, collect cyst nematodes (Heterodera avenae) from an infested field in Qingdao, China. Then, inoculate wild-type and overexpression wheat roots with these nematodes, while maintaining a non-inoculated set of plants as a negative control. Finally, collect all root samples three days after inoculation. Total RNA was extracted using TRIzol reagent (Invitrogen, catalogue number 15596018). First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara).  First, fragmented DNA undergoes end repair and A-tailing to create compatible ends. Then, sequencing adapters are ligated to the fragments, followed by purification and PCR amplification to generate the final library.
attributes.tag: ENA first public
attributes.value: 2025-11-25
attributes.tag: INSDC center name
attributes.value: Institute of Plant Protection
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15943:WT_3dpi_3
attributes.tag: age
attributes.value: 17
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: bread wheat
attributes.tag: cultivar
attributes.value: Jimai22
attributes.tag: developmental stage
attributes.value: seedling development stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: infect
attributes.value: Heterodera avenae
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: root
attributes.tag: sample name
attributes.value: E-MTAB-15943:WT_3dpi_3
attributes.tag: scientific_name
attributes.value: Triticum aestivum