organism: Homo sapiens
description: Protocols: HepG2 cell line was ordered from ATCC (HB-8065) and cultured. Cells were harvested directly from cell culture and no dissection was perormed. HepG2 cells were cultivated in DMEM (Gibco, 41965) containing 10% FBS (Gibco, 10500064) and 1% penicillin/streptomycin (Gibco, 15140122) Cells were treated with treated with GW3965 (5µM), GW4064 (5 µM) alone or combined with mTORC1 inhibitor Torin 1 (TOI), or combined with glucose/glutamine starved media (GLUST). DMSO with final concentration of 0.1% (v/v) was used as control treatment.  RNA was extracted using Trizol reagent (Invitrogen, 15596026) according to the manufacturer's instructions. QuantSeq 3' mRNA library preparation kit (Lexogen) according to manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2025-12-16
attributes.tag: INSDC center name
attributes.value: Institute of Bioinformatics, Biocenter Medical University of Innsbruck
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16150:HEPG2_11_GW3965_TOI_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HepG2
attributes.tag: cell type
attributes.value: hepatocyte
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adolescent
attributes.tag: disease
attributes.value: hepatocellular carcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-16150:HEPG2_11_GW3965_TOI_1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: stimulus
attributes.value: DMEM, 10% FBS, 1% penicillin/streptomycin