organism: Mus musculus
attributes.tag: sample_name
attributes.value: Fucci_ESC_Async_R1
attributes.tag: cell_line
attributes.value: R26p-Fucci2 mESC
attributes.tag: cell_type
attributes.value: embryonic stem cells
attributes.tag: collection_date
attributes.value: missing: lab stock
attributes.tag: dev_stage
attributes.value: embryonic
attributes.tag: geo_loc_name
attributes.value: missing: lab stock
attributes.tag: growth_protocol
attributes.value: Mouse embryonic stem cells were maintained in 2i/LIF medium consisting of NDiff 227 base medium supplemented with the two inhibitors (2i) PD0325901 (1 uM) and CHIR99021 (3 uM), plus leukemia inhibitory factor (LIF; 1000 U/mL) on iMatrix-511-coated dishes. Cells were cultured at 37 C with 5% CO2 and passaged every 2-3 days using trypsin/EDTA.
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: BDF1 (C57BL/6N x DBA/2N)
attributes.tag: tissue
attributes.value: embryonic stem cells
attributes.tag: treatment
attributes.value: Fixation with 1% formaldehyde followed by FACS sorting