organism: marine metagenome
description: This sample represents seawater environmental DNA (eDNA) collected from the intertidal zone at Molhe Beach, Porto, northern Portugal. The sample was used for 16S rRNA gene amplicon sequencing on the Ion Torrent S5 platform (Thermo Fisher Scientific) using the Ion 16S Metagenomics Kit, which targets six hypervariable regions (V2, V3, V4, V6-7, V8, and V9) of the bacterial 16S rRNA gene. DNA was extracted directly from the egg string material and processed according to the manufacturer's protocol. Taxonomic classification of the resulting reads was performed using the Ion Reporter 16S Metagenomics workflow, applying a two-step BLAST alignment against the MicroSEQ ID bacterial reference library and the Greengenes database, following Clinical and Laboratory Standards Institute (CLSI) thresholds for identity (less than 97 percent for family level, greater than 97 percent for genus level, and greater than 99 percent for species level). The dataset is part of a study investigating the bacterial communities associated with Aplysia depilans egg strings and the surrounding marine environment. The BioProject includes raw sequence reads and corresponding sample metadata for public reuse and comparative analyses.
attributes.tag: collection_date
attributes.value: 2025-08/2025-09
attributes.tag: depth
attributes.value: 0-1 m
attributes.tag: env_broad_scale
attributes.value: marine biome [ENVO:00000447]
attributes.tag: env_local_scale
attributes.value: oastal marine habitat [ENVO:01000048]|intertidal zone [ENVO:00000316]
attributes.tag: env_medium
attributes.value: seawater [ENVO:00002149]|marine water [ENVO:00002113]
attributes.tag: geo_loc_name
attributes.value: Portugal: Molhe beach, Porto
attributes.tag: lat_lon
attributes.value: 41.1580 N 8.6844 W
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.water