organism: Mus musculus
description: Protocols: For platelets (PLT) isolation, whole blood was collected to acid-citrate-dextrose tubes supplemented with 3 uM prostaglandin E1 (PGE), and PLT-rich plasma was collected by centrifugation. Pelleted PLTs were subjected to red cell lysis and resuspended in the presence of anti-CD45 beads (Milteney Biotec) to remove residual leukocytes. Total RNA was isolated using RNeasy Micro Kit (Qiagen) as described in the manufacturer's protocol. Libraries were prepared with a SMARTer® Stranded Total RNA-Seq – Pico Input Mammalian library preparation kit v2 (Takara).
attributes.tag: ENA first public
attributes.value: 2025-08-22
attributes.tag: INSDC center name
attributes.value: Institute of Molecular Genetics of the Czech Academy of Sciences
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14685:PLT_MUT3
attributes.tag: age
attributes.value: 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: platelet
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: homozygous Jak2 R1063H
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: C
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-14685:PLT_MUT3
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6