organism: Mycobacterium tuberculosis H37Rv
description: Protocols: WT and ΔsubI H37Rv were grown in triplicates in 7H9 medium supplemented with 10% ADC, 0,05% Tyloxapol, 1mM MgSO4, and 1mM cysteine until OD600 =0.8.   Total RNA was extracted from 10 mL of bacterial cultures grown to logarithmic phase (OD600nm = 0.8) using RNeasy kit (Qiagen).  Total Bulk RNA sequencing was performed Each extracted total RNA sample was barcoded with CEL-seq primers during the enzymatic lysis. It was then followed by fragmentation, end repair, poly(A)-tailing, reverse transcription, and second-strand synthesis. After second-strand synthesis, the barcoded samples were pooled into one library and amplified using in vitro transcription. Further, ribosomal aRNA was depleted. After depletion, adapter ligation, reverse transcription, and indexing PCR were performed to prepare a cDNA library.
attributes.tag: ENA first public
attributes.value: 2025-09-15
attributes.tag: INSDC center name
attributes.value: Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15580:SubI_KO_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: genotype
attributes.value: subI KO
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sample name
attributes.value: E-MTAB-15580:SubI_KO_2
attributes.tag: scientific_name
attributes.value: Mycobacterium tuberculosis H37Rv