title: Metagenome-assembled genome: SRP262948_co1_150
organism: uncultured Candidatus Obscuribacterales bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR11830853,SRR11830854 of study SRP262948.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: SRP262948_co1_150
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: engineered
attributes.tag: collection date
attributes.value: 2016-06-21
attributes.tag: completeness score
attributes.value: 91.17
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 2.81
attributes.tag: environmental medium
attributes.value: engineered
attributes.tag: geographic location (country and/or sea)
attributes.value: China
attributes.tag: geographic location (latitude)
attributes.value: 22.59
attributes.tag: geographic location (longitude)
attributes.value: 113.97
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: activated sludge metagenome
attributes.tag: local environmental context
attributes.value: activated sludge biome
attributes.tag: metagenomic source
attributes.value: activated sludge metagenome
attributes.tag: project name
attributes.value: we used metagenomic assembly and binning methods to decipher and compare the prevalence of ARGs and their potential mobility and hosts in AS reactors treating antibiotic wastewater. To do this, we operated lab-scale sequencing batch reactors of AS process by independently adding five antibiotics of cephalexin, chloramphenicol, kanamycin, sulfamethazine and tetracycline with high concentrations. Specifically, the objectives were (1) to reveal the co-selection effects of different antibiotics for antibiotic resistance by investigating the dynamics of co-existence between ARGs, (2) to evaluate the potential mobility of ARGs by identifying the co-occurrence patterns of ARGs and MGEs, and (3) to explore the host-association of ARGs by recovering large number of metagenome-assembled genomes from AS samples.
attributes.tag: sample derived from
attributes.value: SAMN14996806,SAMN14996805
attributes.tag: sample name
attributes.value: SRP262948_co1_150
attributes.tag: scientific_name
attributes.value: uncultured Candidatus Obscuribacterales bacterium
attributes.tag: sequencing method
attributes.value: Illumina HiSeq 4000
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach