title: 22EL-RB475-20
organism: seawater metagenome
attributes.tag: ENA first public
attributes.value: 2025-06-11
attributes.tag: INSDC center name
attributes.value: Ifremer
attributes.tag: INSDC status
attributes.value: public
attributes.tag: LIBRARY_CONSTRUCTION_PROTOCOL
attributes.value: Production of targeted PCR from 10 ng of DNA material. The aim is to generate amplified targeted regions indexed with secondary identifiers or BID = Barcoded Identifiers. In the protocol, the region of interest is amplified using PCR Forward and Reverse primers flanked by sequences of 8 bases, called "BID" (the name has been developed at Genoscope to differentiate from other MIDs = Molecular Identifiers already in use at the time). To date, 18 different BID sequences have been tested, 12 of which have been selected. The amplicons generated will be then normalized and pooled by up to 12 (amplicons carrying different BID sequences). Illumina libraries will be prepared by Genoscope on each pool of BIDed amplicons and then Illumina sequenced
attributes.tag: Submitter Id
attributes.value: sam_BIOCEAN5D-zooplankton_22EL-RB475-20
attributes.tag: broad-scale environmental context
attributes.value: neritic epipelagic zone biome
attributes.tag: broker name
attributes.value: SeBiMER
attributes.tag: collected_by
attributes.value: PDG-ODE-LITTORAL-LERBO
attributes.tag: collecting institution
attributes.value: IFREMER
attributes.tag: collection date
attributes.value: 2022-08-29
attributes.tag: collector name
attributes.value: PDG-ODE-LITTORAL-LERBO
attributes.tag: common name
attributes.value: seawater metagenome
attributes.tag: depth
attributes.value: -1
attributes.tag: environmental medium
attributes.value: seawater
attributes.tag: environmental package
attributes.value: water
attributes.tag: environmental_sample
attributes.value: Yes
attributes.tag: experimental factor
attributes.value: sampling site,time,distance from the coast
attributes.tag: geographic location (country and/or sea)
attributes.value: France
attributes.tag: geographic location (elevation)
attributes.value: not provided
attributes.tag: geographic location (latitude)
attributes.value: 48.3094
attributes.tag: geographic location (longitude)
attributes.value: -4.4485
attributes.tag: geographic location (region and locality)
attributes.value: Rade de Brest
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: isolation_source
attributes.value: offshore sea water
attributes.tag: local environmental context
attributes.value: bay
attributes.tag: multiplex identifiers
attributes.value: TACTATAC
attributes.tag: pcr primers
attributes.value: F: GGWACWGGWTGAACWGTWTAYCCYCC / R: TAIACYTCIGGRTGICCRAARAAYCA
attributes.tag: project name
attributes.value: ROME
attributes.tag: sample name
attributes.value: sam_BIOCEAN5D-zooplankton_22EL-RB475-20
attributes.tag: sample_description
attributes.value: Sample 475 of offshore sea water from Rade de Brest, collected in 2022, filtered on 20 micrometers filter. Sequenced for zooplankton analysis purpose.
attributes.tag: sample_name
attributes.value: 22EL-RB475-20
attributes.tag: scientific_name
attributes.value: seawater metagenome
attributes.tag: target gene
attributes.value: COX1
attributes.tag: target subfragment
attributes.value: not applicable