title: M09_D0
organism: Homo sapiens
description: Protocols: Whole blood was collected from volunteers at -D3 (baseline), D0 (before vaccination), D1, D3, D7, and D10 post-vaccination and stored in Tempus blood RNA tubes (Thermofisher) at -80°C until use. Tempus blood RNA tubes were thawed at room temperature, and RNA was extracted using the Tempus spin RNA isolation kit (Thermofisher, catalog nunmber: 4380204), eluted in 90µL of RNase-free water according to the manufacturer's protocol. 1µg of total RNA was used for library preparation. poly(A) mRNA isolation was performed using Oligo(dT) beads. mRNA fragmentation was carried out using divalent cations and high temperature. Priming was performed using random primers. First strand cDNA and the second-strand cDNA were synthesized. The purified double-stranded cDNA was then treated to repair both ends and add a dA-tailing in one reaction, followed by T-A ligation to add adaptors to both ends. Size selection of adaptor-ligated DNA was then performed using DNA clean beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated.
attributes.tag: ENA first public
attributes.value: 2024-01-01
attributes.tag: External Id
attributes.value: SAMEA114268318
attributes.tag: INSDC center name
attributes.value: Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore
attributes.tag: INSDC last update
attributes.value: 2023-08-15T16:20:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13265:M09_D0
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical history
attributes.value: YF-17D vaccinatio
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: M09
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-13265:M09_D0
attributes.tag: scientific_name
attributes.value: Homo sapiens