organism: Mus musculus
description: Each sample was uniquely labeled with BioLegend anti-mouse hashtag antibodies (A0301–A0308) prior to pooling, with HTO barcode sequences assigned as follows: CTRL1 (A0301) ACCCACCAGTAAGAC; CTRL2 (A0302) GGTCGAGAGCATTCA; CTRL3 (A0303) CTTGCCGCATGTCAT; CTRL4 (A0304) AAAGCATTCTTCACG; TREAT1 (A0305) CTTTGTCTTTGTGAG; TREAT2 (A0306) TATGCTGCCACGGTA; TREAT3 (A0307) GAGTCTGCCAGTATC; TREAT4 (A0308) TATAGAACGCCAGGC. After sequencing, pooled cells were demultiplexed based on HTO counts to assign cell barcodes and identify singlets/doublets.
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: age
attributes.value: 10 weeks
attributes.tag: collection_date
attributes.value: 2025-04
attributes.tag: geo_loc_name
attributes.value: USA: Texas, Houston
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: Subcutaneous tumor
attributes.tag: treatment
attributes.value: pooled-cDNA
attributes.tag: BioSampleModel
attributes.value: Model organism or animal