title: ZAP_CpG_1
organism: Homo sapiens
description: Protocols: Cells were infected at MOI=3. The medium was replaced 18 hours (h) post inoculation. Cell lysates and supernatants were collected at 48 hours post-infection. Supernatants were spun down at 300 ×g for five minutes and analysed by TZM-bl assay and immunoblotting. Total RNA was extracted using the Direct-zol RNA Miniprep Plus Kit The total RNA was purified with the Monarch Spin RNA Cleanup Kit (NEB). The 5' RACE was performed from 500 ng purified RNA using the SMARTer RACE 5'/3' Kit and according to the manufacturer's instructions. 5' RACE sequencing librairies were prepared using the Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies).
attributes.tag: ENA first public
attributes.value: 2025-09-30
attributes.tag: INSDC center name
attributes.value: UK Dementia Research Institute, King's College London, London, UK
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15095:ZAP_CpG_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HeLa
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: genotype
attributes.value: ZAP_CpG
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sample name
attributes.value: E-MTAB-15095:ZAP_CpG_1
attributes.tag: scientific_name
attributes.value: Homo sapiens