title: Metagenome-assembled genome: ERP119702_co2_74
organism: uncultured Candidatus Saccharibacteria bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic run ERR3856788 of study ERP119702.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: ERP119702_co2_74
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT2;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: aquatic
attributes.tag: collection date
attributes.value: 2016-09-14
attributes.tag: completeness score
attributes.value: 100
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 0.13
attributes.tag: environmental medium
attributes.value: aquatic
attributes.tag: geographic location (country and/or sea)
attributes.value: Switzerland
attributes.tag: geographic location (latitude)
attributes.value: 47.09250000
attributes.tag: geographic location (longitude)
attributes.value: 8.48910000
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: freshwater metagenome
attributes.tag: local environmental context
attributes.value: freshwater biome
attributes.tag: metagenomic source
attributes.value: freshwater metagenome
attributes.tag: project name
attributes.value: DNA and total RNA was extracted from water from of the anoxic hypolimnion of a freshwater lake in two years (2016, 2018). DNA was used for metagenomic sequencing (2x250 bp, Illumina HiSeq 2500). For metatranscriptome sequencing, total RNA was subjected to rRNA depletion followed by reverse transcription and sequencing of cDNA (1x150 bp, Illumina HiSeq 3000).
attributes.tag: sample derived from
attributes.value: SAMN07599903
attributes.tag: sample name
attributes.value: ERP119702_co2_74
attributes.tag: scientific_name
attributes.value: uncultured Candidatus Saccharibacteria bacterium
attributes.tag: sequencing method
attributes.value: Illumina HiSeq 2500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach