title: N3_mNLS_LPS
organism: Mus musculus
description: Protocols: Primary BMDMs from WT or mNLS mice were treated with vehicle or LPS (1ug/ml, 4h). Supernatant was aspirated, cells washed once with PBS, and then lysis buffer containing 1% mercaptoethanol was added. Total RNA was extracted using a PureLink RNA mini kit. Illumina TruSeq Stranded mRNA kit, according to the manufacturer's protocol.
attributes.tag: ENA first public
attributes.value: 2026-02-28
attributes.tag: INSDC center name
attributes.value: The University of Manchester
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16641:N3_mNLS_LPS
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: Primary bone marrow-derived macrophages
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: mNLS
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: replicate
attributes.value: 3
attributes.tag: sample name
attributes.value: E-MTAB-16641:N3_mNLS_LPS
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: stimulus
attributes.value: LPS
attributes.tag: strain
attributes.value: C57BL/6J