title: Metagenome-assembled genome: binchicken_co165_411
organism: uncultured Nitrospirota bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR12685295,SRR9952630 of study SRP284337.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: binchicken_co165_411
attributes.tag: assembly quality
attributes.value: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs
attributes.tag: assembly software
attributes.value: MEGAHIT;1.2.9;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: VAMB;3.0.2;default
attributes.tag: broad-scale environmental context
attributes.value: aquatic
attributes.tag: collection date
attributes.value: not provided
attributes.tag: completeness score
attributes.value: 98.31
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 0.15
attributes.tag: environmental medium
attributes.value: aquatic
attributes.tag: geographic location (country and/or sea)
attributes.value: not applicable
attributes.tag: geographic location (latitude)
attributes.value: not provided
attributes.tag: geographic location (longitude)
attributes.value: not provided
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: freshwater sediment metagenome
attributes.tag: local environmental context
attributes.value: freshwater sediment biome
attributes.tag: metagenomic source
attributes.value: freshwater sediment metagenome
attributes.tag: project name
attributes.value: Microbially mediated sulfate reduction in aquatic sediments mineralizes organic carbon, generates hydrogen sulfide, and mediates the geochemical cycles of other elements, such as iron, phosphorus, and mercury. While organic matter contains a number of sulfur compounds, little is known of the fate of this organic sulfur pool during mineralization and more importantly its contribution to the inorganic sulfur cycle that fuels sulfate reduction. The current paradigm of sulfate reduction involves diffusion of sulfur from overlying water into the sediments where sulfur-reducing microorganisms are present. Contrary to the paradigm, modeling and preliminary results demonstrate that under low-sulfate conditions organic sulfur buried in sediment may be the dominant source of sulfur for sulfate reduction, and once mobilized, via microbial biotransformation, may be exported to the overlying water column. Contributions from organic sulfur may be pervasive in environments such as oligotrophic freshwater lakes or the oceans of the geologic past. By characterizing the organic sulfur transformations in sediments across a range of sulfate and organic carbon levels in Lake Superior and its largest American tributary, investigators will address the following questions under a range of environmental conditions: A. To what extent does organic sulfur contribute to the pool of sulfur that fuels sulfate reduction? B. Does organic sulfur undergo cryptic, microbially-mediated biogeochemical transformations, and what microorganisms are responsible for these transformations? They have assembled a multidisciplinary research team that combines expertise in sediment geochemistry, organic geochemistry, and geomicrobiology to address these objectives using sediment characterizations, rate measurements, molecular characterizations of microbial communities, and modeling. The results will quantify an important part of the diagenetic sulfur cycle that has received little attention despite its potential significance in environments such as freshwater lakes, deep subsurface, and the low sulfate oceans of the geological past. Verifying the proposed hypotheses may lead to reevaluation of the geochemical cycles of sulfur and associated elements such as iron and nitrogen, including cryptic reactions in the sulfate-methane transition zone reinterpretation of the origins of the isotopic signatures of sulfur preserved in both modern aquatic sediments and ancient sedimentary rocks and conservation and management practices in sulfide affected water bodies. The project will generate novel microbial and geochemical data that will be publicly available.
attributes.tag: sample derived from
attributes.value: SAMN16212730,SAMN12368158
attributes.tag: sample name
attributes.value: binchicken_co165_411
attributes.tag: scientific_name
attributes.value: uncultured Nitrospirota bacterium
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000,Illumina HiSeq 2500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach