title: FVV32
organism: Homo sapiens
description: Protocols: We centrifuged umbilical cord blood and separated plasma for storage at -80°C. After density gradient centrifugation, the cord blood mononuclear cell fraction was plated at 5 x 107 cells/well on collagen coated 6-well plates ((BioCoat; Corning, USA) and incubated at 37 °C and 5 % CO2. Cells were grown with endothelial basal medium (EBM-2, Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS, Harvard Bioscience, Harvard Bioscience, Holliston, USA), 1% penicillin/streptomycin (P/S, Bio&Sell, Nürnberg, Germany) and recommended supplements. After 10 to 21 days, ECFC colonies appeared as adherent single layers of cobblestone-shaped cells. ECFC phenotype was confirmed by flow cytometry assessing CD31 (130-117-390; BD Biosciences San Jose, USA), CD45 (555483; BD Biosciences), CD133 (130-090-826; Miltenyi Biotec, Bergisch Gladbach, Germany) and the corresponding isotype controls. Total RNA was purified from ECFC lysates using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). rRNA was depleted and PolyA enriched without globin depletion. A strand-specific library was utilized with the protocol NEB Ultra Directional RNA.
attributes.tag: ENA first public
attributes.value: 2025-08-07
attributes.tag: INSDC center name
attributes.value: Hannover Medical School
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14356:FVV32
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: endothelial colony forming cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: Preeclampsia
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: umbilical cord blood
attributes.tag: sample name
attributes.value: E-MTAB-14356:FVV32
attributes.tag: scientific_name
attributes.value: Homo sapiens