title: HT-4
organism: Mus musculus
description: Protocols: The thyroid tissue was very gently dissociated into small pieces and incubated with collagenase D and DNase I for 30 min at 37°C under mild agitation. Then, a filtration step with a 0.70 µm pore size filter is applied to remove the largest elements. The remaining liquid was differentially centrifuged at 300 × g for 10 min and 2,000 × g for 20 min to remove cells and tissue debris. The supernatant was then further centrifuged at 16,500 × g for 20 min and at 118,000 × g for 2.5 h to collect the sEV. Total RNA from cell or tissue sEV was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The RNA concentration was confirmed using absorbance measurements with a NanoDrop 2100 (Thermo Scientific). A total amount of 1ng-500ng RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using QIAseq miRNA Library Kit (Qiagen, Frederick, MD) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
attributes.tag: ENA first public
attributes.value: 2026-01-18
attributes.tag: External Id
attributes.value: SAMEA112661861
attributes.tag: INSDC center name
attributes.value: West China Hospital
attributes.tag: INSDC last update
attributes.value: 2023-02-20T13:03:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12659:HT-4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: Hashimoto's thyroiditis
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: thyroid gland
attributes.tag: sample name
attributes.value: E-MTAB-12659:HT-4
attributes.tag: sampling site
attributes.value: extracellular vesicle
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: NOD.Cg-H2h4/DilTacUmmJ