title: RNA6hCSS66_libLAC3050
organism: Staphylococcus aureus
description: Protocols: S. aureus isolates from chronic rhinosinusitis patients (CSS) and healthy individuals (MIN) were cultured overnight.Cultures were diluted in TSB broth to an optical density (OD600) of 0.05 the following day, and samples were collected at 3- and 6-hours post-dilution.  RNA from harvested samples were isolated using Zymo Research Quick-RNA Fungal/Bacterial Miniprep Kit standard protocol and rRNA was depleted using Invitrogen MICROBExpress™ Bacterial mRNA Enrichment Kit kit. Nucleic acid library was prepared using standard Illumina RNA-seq protocol that uses random hexamer primers that for cDNA synthesis.
attributes.tag: ENA first public
attributes.value: 2026-02-12
attributes.tag: INSDC center name
attributes.value: Institute for Infectious Diseases and Infection Control, Jena University Hospital, Jena, Germany
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16578:RNA6hCSS66_libLAC3050
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical information
attributes.value: chronic rhinosinusitis patients (CSS)
attributes.tag: collection date
attributes.value: not collected
attributes.tag: disease
attributes.value: chronic rhinosinusitis
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-16578:RNA6hCSS66_libLAC3050
attributes.tag: scientific_name
attributes.value: Staphylococcus aureus
attributes.tag: strain
attributes.value: CSS66