title: Sample 5
organism: Mus musculus
description: Protocols: Freshly isolated small intestinal crypts were cultured as organoids in 60% matrigel for 48 hours, with or without recombinant asporin. Organoids were collected from matrigel and total RNA was extracted from them. Total RNA was isolated using RNeasy Mini Kit (Qiagen) according to the Manufacturer's instructions with on-column DNAse digestion. An Ovation Universal RNA-seq System kit was used for Illumina library preparations (NuGEN Technologies Inc., CA, USA), with rRNA removal according to the kit manual. Libraries were purified with AMPure XP beads.
attributes.tag: ENA first public
attributes.value: 2025-11-27
attributes.tag: INSDC center name
attributes.value: KIS
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14662:Sample 5
attributes.tag: age
attributes.value: 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: 3
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: epithelium of small intestine
attributes.tag: sample name
attributes.value: E-MTAB-14662:Sample 5
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J