title: Metagenome-assembled genome: binchicken_co168_331
organism: uncultured Bacillaceae bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3839023,SRR5581382 of study ERP119604.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: binchicken_co168_331
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: VAMB;3.0.2;default
attributes.tag: broad-scale environmental context
attributes.value: engineered
attributes.tag: collection date
attributes.value: not provided
attributes.tag: completeness score
attributes.value: 58.83
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 0.0
attributes.tag: environmental medium
attributes.value: engineered
attributes.tag: geographic location (country and/or sea)
attributes.value: not applicable
attributes.tag: geographic location (latitude)
attributes.value: not provided
attributes.tag: geographic location (longitude)
attributes.value: not provided
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: activated sludge metagenome
attributes.tag: local environmental context
attributes.value: activated sludge biome
attributes.tag: metagenomic source
attributes.value: activated sludge metagenome
attributes.tag: project name
attributes.value: Bioelectrochemical systems (BES) exploit the interaction between microbes andelectrodes. A field of application thereof, is bioelectrochemical remediation, an effective strategy inenvironments where the absence of suitable electron acceptors limits classic bioremediationapproaches. Understanding the microbial community structure and genetic potential of anodebiofilms is of great interest to interpret the mechanisms occurring in BES. In this study, by using awhole metagenome sequencing approach, taxonomic and functional diversity patterns in theinoculum and on the anodes of three continuous-flow BES for the removal of phenol, toluene andBTEX were obtained. The genus Geobacter was highly enriched on the anodes and tworeconstructed genomes were taxonomically related to the Geobacteraceae family. To functionallycharacterize the microbial community, the genes coding for the anaerobic degradation of toluene,ethylbenzene and phenol were selected as genetic markers for the anaerobic degradation of thepollutants. The genes related with direct extracellular electron transfer (EET) were also analyzed.The inoculum carried the genetic baggage for the degradation of aromatics but lacked the capacityof EET, while anodic bacterial communities were able to pursuit both processes. Themetagenomic approach provided useful insights into the ecology and complex functions withinhydrocarbon degrading electrogenic biofilms.
attributes.tag: sample derived from
attributes.value: SAMEA6502094,SAMN07139232
attributes.tag: sample name
attributes.value: binchicken_co168_331
attributes.tag: scientific_name
attributes.value: uncultured Bacillaceae bacterium
attributes.tag: sequencing method
attributes.value: Illumina HiSeq 2000,Illumina HiSeq 4000
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach