title: WT_1_IAV
organism: Mus musculus
description: Protocols: Infected WT, mNLS, and heterozygous control mice were culled on day 7 post-IAV X31 (H3N2) infection. One lung lobe was collected and placed in RNAlater stabilisation solution prior to subsequent RNA extraction.  Total RNA was extracted by transferring to a 2 mL tube containing RLT buffer supplemented with β-mercaptoethanol and a single 2 mm stainless steel bead. Tissue was homogenised into suspension using a Qiagen Tissue Lyser. Total RNA was then extracted using the RNeasy Mini Kit according to the manufacturer's protocol.  Illumina TruSeq Stranded mRNA kit, according to the manufacturer's protocol.
attributes.tag: ENA first public
attributes.value: 2026-02-28
attributes.tag: INSDC center name
attributes.value: The University of Manchester
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16640:WT_1_IAV
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: Influenza A
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: 4
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-16640:WT_1_IAV
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J