organism: chicken gut metagenome
attributes.tag: collection_date
attributes.value: 2024-04
attributes.tag: env_broad_scale
attributes.value: gallus gallus biome [ENVO:00000428]
attributes.tag: env_local_scale
attributes.value: intestine [ENVO:00001994]
attributes.tag: env_medium
attributes.value: intestinal content [ENVO:00002245]
attributes.tag: geo_loc_name
attributes.value: China
attributes.tag: host
attributes.value: Gallus gallus
attributes.tag: lat_lon
attributes.value: 37.092754 N 82.708184 E
attributes.tag: biosample_accession
attributes.value: HH3I.27F_1492R.
attributes.tag: library_id
attributes.value: HH3I.27F_1492R.
attributes.tag: title
attributes.value: 16S rDNA sequencing of hen: intestinal contents of adult hens
attributes.tag: library_strategy
attributes.value: AMPLICON
attributes.tag: library_source
attributes.value: GENOMIC
attributes.tag: library_selection
attributes.value: PCR
attributes.tag: library_layout
attributes.value: paired
attributes.tag: platform
attributes.value: PACBIO_SMRT
attributes.tag: instrument_model
attributes.value: PacBio RS II
attributes.tag: design_description
attributes.value: DNA extraction was performed using the QIAamp DNA Stool Mini Kit (Qiagen Inc., Valencia, CA) according to the manufacturer's instructions.Third-generation sequencing technology was employed for full-length sequencing of the 16S rRNA gene. The primers 27F/1492R were used to amplify and sequence the full-length 24S rRNA gene.For single-molecule real-time sequencing (SMRT sequencing) on the PacBio RS II sequencing platform (Pacific Biosciences, Menlo Park, CA). The sequencing data were initially processed using the SMRT Portal software, which included the removal of adapter sequences and filtering of low-quality reads.
attributes.tag: filetype
attributes.value: fastq
attributes.tag: filename
attributes.value: HH3I.27F_1492R.raw.fastq.gz
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated