title: Sample7
organism: Homo sapiens
description: Protocols: Cells were grown according to the protocol in DOI 10.1385/1-59259-861-7:183 Passage 2 primary human bronchial epithelial (HBE) cells  were cultured in air-liquid interface (ALI) culture for up to 14 days in culture. HBE cells were grown normally according to the growth protocol above and frozen on ALI days 0, 1, 7, and 14 to allow for RNA extraction. Total RNA was isolated from HBE cells frozen on ALI days 0, 1, 7, and 14 using the TRIzol Plus RNA Purification Kit (Invitrogen) Sequencing libraries were constructed with a Watchmaker Genomics kit.
attributes.tag: ENA first public
attributes.value: 2025-11-26
attributes.tag: INSDC center name
attributes.value: BIODK
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16209:Sample7
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Primary cells
attributes.tag: cell type
attributes.value: Human bronchial epithelial cells
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: no lung disease
attributes.tag: genotype
attributes.value: NA
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth condition
attributes.value: VM
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung epithelium
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-16209:Sample7
attributes.tag: scientific_name
attributes.value: Homo sapiens