title: Metagenome-assembled genome: binchicken_co198_17
organism: uncultured Halobacteriales archaeon
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR5202689,SRR5202690 of study SRP097780.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: binchicken_co198_17
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: SemiBin2;2.0.2;default
attributes.tag: broad-scale environmental context
attributes.value: engineered
attributes.tag: collection date
attributes.value: 2015-01-20
attributes.tag: completeness score
attributes.value: 84.37
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 3.08
attributes.tag: environmental medium
attributes.value: engineered
attributes.tag: geographic location (country and/or sea)
attributes.value: United Kingdom
attributes.tag: geographic location (latitude)
attributes.value: 54.5561
attributes.tag: geographic location (longitude)
attributes.value: -0.8216
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: salt mine metagenome
attributes.tag: local environmental context
attributes.value: salt mine biome
attributes.tag: metagenomic source
attributes.value: salt mine metagenome
attributes.tag: project name
attributes.value: We will address the question of how carbon is used in deep subsurface hypersaline microbial communities associated with deep Permian evaporate deposits. Our work involves examining brine seeps present in a UK salt mine (Boulby Mine). Here, we are able to utilize the vast area exposed by the mine roadway network (>1000km) to give us unprecedented access to the deep subsurface. Using this substantial area allows us not only to examine carbon usage in localized areas of the environment, but over extensive regions of the evaporite deposit. Consequently, this project provides a novel opportunity to ask questions about the biogeographical patterns present within a deep subsurface environment. Metagenomic sequencing is a vital to achieving these goals. The two key questions we aim to answer with this sequencing work are therefore:</p><p>What is the primary source of carbon for microbial communities living in a deep subsurface evaporite? and</p><p>Do the metabolic and carbon usage capabilities of these microbial communities vary spatially within a deep subsurface evaporite?
attributes.tag: sample derived from
attributes.value: SAMN06270278,SAMN06270277
attributes.tag: sample name
attributes.value: binchicken_co198_17
attributes.tag: scientific_name
attributes.value: uncultured Halobacteriales archaeon
attributes.tag: sequencing method
attributes.value: NextSeq 500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach