title: I7-1
organism: Gadus morhua
description: Protocols: The fish were sampled at 20 h or 48 h post injection. The fish were killed by a blow to the head. Liver samples were rapidly excised, snap frozen in liquid nitrogen and stored at -80°C for further processing. Liver total RNA extraction was performed from individual fish. A total of 32 individual liver RNA samples from groups of 20 h time point (Control, BPA (40 μmol/kg bw), MXC (40 μmol/kg bw) and EE2 (50 nmol/kg bw), n = 6 per group) and 48 h time point (Control, n = 3, EE2 = 5) were analyzed. RNA extraction was performed from each sample using TRI Reagent (Sigma) according to the manufacturer's protocols. The concentration and quality of total RNA was assessed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), and the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). RNA sequencing was performed at the Genomics Core Facility at the University of Bergen, as previously described (Yadetie et al., 2018), according to Illumina TruSeq® Stranded mRNA Sample Preparation Guide on Illumina HiSeq 4000 (Illumina, Inc., San Diego, CA, USA).
attributes.tag: ENA first public
attributes.value: 2024-01-01
attributes.tag: External Id
attributes.value: SAMEA112641194
attributes.tag: INSDC center name
attributes.value: Department of Biological Sciences University of Bergen, Bergen, Norway
attributes.tag: INSDC last update
attributes.value: 2023-02-13T14:57:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12606:I7-1
attributes.tag: age
attributes.value: 1.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: Atlantic cod
attributes.tag: developmental stage
attributes.value: juvenile stage
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-12606:I7-1
attributes.tag: scientific_name
attributes.value: Gadus morhua
attributes.tag: sex
attributes.value: male