title: Metagenome-assembled genome: ERP119294_co1_436
organism: uncultured Planctomycetota bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3804373,ERR3804374 of study ERP119294.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: ERP119294_co1_436
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: VAMB;3.0.2;default
attributes.tag: broad-scale environmental context
attributes.value: terrestrial
attributes.tag: collection date
attributes.value: not provided
attributes.tag: completeness score
attributes.value: 99.06
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 0.54
attributes.tag: environmental medium
attributes.value: terrestrial
attributes.tag: geographic location (country and/or sea)
attributes.value: not provided
attributes.tag: geographic location (latitude)
attributes.value: not provided
attributes.tag: geographic location (longitude)
attributes.value: not provided
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: sediment metagenome
attributes.tag: local environmental context
attributes.value: sediment biome
attributes.tag: metagenomic source
attributes.value: sediment metagenome
attributes.tag: project name
attributes.value: Lake sediments not only store the long-term ecological information, but are also a source of sedimentary ancient DNA (sedaDNA). Here, by combination of traditional multi-proxy paleolimnological methods with the whole-metagenome shotgun-sequencing of sedaDNA we were able to paint a comprehensive picture of the fluctuations in trophy and microbial diversity and metabolism of a small temperate lake in response to hemp retting, across the past 2000 years. Hemp retting (HR), a key step in hemp fibre production, was historically carried out in freshwater reservoirs and had a negative impact on the lake ecosystems. In Lake Slone, we identified two HR events, during the late stage of Roman and Early Medieval periods, and correlated these to increased trophy and imbalanced lake microbiome. The metagenomic analyses showed a higher abundance of Chloroflexi, Planctomycetes and Bacteroidetes and a functional shift towards anaerobic metabolism, including degradation of complex biopolymers such as pectin and cellulose, during HR episodes. The lake eutrophication during HR was linked to the allochthonous, rather than autochthonous carbon supply – hemp straws. We also showed that the identification of HR based on the palynological analysis of hemp pollen may be inconclusive and we suggest employment of the fibre count analysis as an additional and independent proxy.
attributes.tag: sample derived from
attributes.value: SAMEA6464978,SAMEA6464979
attributes.tag: sample name
attributes.value: ERP119294_co1_436
attributes.tag: scientific_name
attributes.value: uncultured Planctomycetota bacterium
attributes.tag: sequencing method
attributes.value: NextSeq 500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach