title: Sequencing of plasmid pools from Round 6 of evolution after rescreening in DH10B E. coli
organism: Escherichia coli
description: Rubisco GWS1B under T7 promoter was selectively mutated in MutaT7-Dual8 E. coli for 24h per round of directed evolution. Two plasmids were present in the starting culture: BAC-GWS1B containing Rubisco and pACYC-prk::nptII to enable Rubisco selection. Both plasmids were purified from all cell material on a selective agar plate and sequenced after each evolution round (excluding 5 and 7). Rescreened plasmid pools were selectively digested, retransformed into E. coli, selected on agar plates, and sequenced again.
attributes.tag: strain
attributes.value: MutaT7-Dual8
attributes.tag: host
attributes.value: Gallionellaceae
attributes.tag: collection_date
attributes.value: 2024-04-08
attributes.tag: geo_loc_name
attributes.value: USA:Cambridge
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: mating_type
attributes.value: Round_6_Rescreen
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental