title: Metagenome-assembled genome: SRP185896_co2_199
organism: uncultured Salinivenus sp.
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR14300214,SRR14300223 of study SRP185896.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: SRP185896_co2_199
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: terrestrial
attributes.tag: collection date
attributes.value: 2016-09-04
attributes.tag: completeness score
attributes.value: 78.73
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 2.45
attributes.tag: environmental medium
attributes.value: terrestrial
attributes.tag: geographic location (country and/or sea)
attributes.value: USA
attributes.tag: geographic location (latitude)
attributes.value: not provided
attributes.tag: geographic location (longitude)
attributes.value: not provided
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: sediment metagenome
attributes.tag: local environmental context
attributes.value: sediment biome
attributes.tag: metagenomic source
attributes.value: sediment metagenome
attributes.tag: project name
attributes.value: Environmental DNA sequencing of archaeal and bacterial 16S rRNA genes was conducted on samples from multiple evaporite sediment layers of the surface salt crust. Our results show that at the time of sampling (September 2016), BSF hosted a robust microbial community dominated by diverse Halobacteriaceae and Salinibacter species. Desulfuromonadales from GR-WP33-58 are also abundant in all samples. We identified taxonomic groups enriched in each layer of the salt crust sediment and revealed that the upper gypsum sediment layer found immediately under the uppermost surface halite contains a robust microbial community. We found an increased presence of Thermoplasmatales, Nanohaloarchaeota, Woesearchaeota, Acetothermia, Halanaerobium, Parcubacteria, Planctomycetes, Clostridia, Gemmatimonadetes, Marinilabiaceae and other Bacteroidetes in this upper gypsum layer. This study provides insight into the diversity, spatial heterogeneity, and geologic context of a surprisingly complex microbial ecosystem within this macroscopically-sterile landscape.</p><p>PIs: Brenda Bowen, William Brazelton
attributes.tag: sample derived from
attributes.value: SAMN10928284,SAMN10928289
attributes.tag: sample name
attributes.value: SRP185896_co2_199
attributes.tag: scientific_name
attributes.value: uncultured Salinivenus sp.
attributes.tag: sequencing method
attributes.value: Illumina HiSeq 2500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach