title: mut205
organism: Mus musculus
description: Protocols: The dorsal telencephalon of E12.5 mouse embryos were collected in 1.5ml Eppendorff tubes and was shock frozen on dry ice and stored at -80C.  Total RNA was extracted using the QIAGEN RNeasy Plus Micro kit as per manufacturer's instructions. RNA was eluated in 14 μl of RNAse free water.  Libraries were prepared from 1 μg of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) and the Poly-A mRNA magnetic isolation module (NEB #E7490)
attributes.tag: ENA first public
attributes.value: 2024-01-01
attributes.tag: INSDC center name
attributes.value: University of Edinburgh Centre for Discovery Brain Sciences
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14015:mut205
attributes.tag: age
attributes.value: 12.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: E12.5
attributes.tag: genotype
attributes.value: mutant genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: mut205
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: dorsal telencephalon
attributes.tag: sample name
attributes.value: E-MTAB-14015:mut205
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: unknown
attributes.tag: strain
attributes.value: C57BL6