title: KO1
organism: Mus musculus
description: Protocols: Mice were euthanized by cervical dislocation at 12 weeks of age. The liver tissue was immediately dissected, rapidly frozen in liquid nitrogen, and subsequently stored at -80°C. Animal samples were extracted using the Trizol method. The extracted RNA was dissolved in 50 μL of DEPC-treated water. The quantity and quality of the total RNA were assessed using a Qubit fluorometer and a Qsep400 high-throughput biofragment analyzer. Strand-specific RNA-seq libraries were prepared. Briefly, mRNA was enriched from total RNA using Oligo(dT) magnetic beads. The purified mRNA was fragmented using fragmentation buffer at an elevated temperature. First-strand cDNA was synthesized using random hexamer primers. Second-strand cDNA was synthesized incorporating dUTP in place of dTTP to achieve strand specificity, followed by end repair, A-tailing, and adapter ligation. Libraries were size-selected for insert sizes of 250-350 bp using DNA magnetic beads and then amplified by PCR. Final library quality was assessed using a Qubit fluorometer and a Qsep400 analyzer. For the DNB platform, the double-stranded DNA library was circularized to form single-stranded circular DNA, which was then rolled-circle amplified to produce DNA nanoballs (DNBs).
attributes.tag: ENA first public
attributes.value: 2025-11-26
attributes.tag: INSDC center name
attributes.value: Xiamen university
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16271:KO1
attributes.tag: age
attributes.value: 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: diet
attributes.value: fasting
attributes.tag: disease
attributes.value: hyperuricemia
attributes.tag: genotype
attributes.value: uox-/-
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: KO1
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-16271:KO1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J