title: Control_1
organism: Mus musculus
description: Protocols: After mouse sacrifice hearts were excised and retrograde perfused with first 5ml Heparin/PBS solution followed by 1ml PBS. Perfused hearts were place in OCT, snap-frozen in pre-cooled isopentane (-30 to -40°C) and stored at -80°C until further use. 10µm thick tissue sections were cut in a cryostat at -20°C chamber temperature and -10°C object head temperature and placed at the 10X Visium Gene Expression slide. Prepared Visium slides were used as input for the Visium Spatial Gene Expression workflow (10X Genomics, Pleasanton, USA) according to the manufacturer's instructions. Tissue permeabilization was carried out for 12 minutes Library construction was performed to manufacturer's instructions, the Visium Spatial Gene Expression workflow (10X Genomics, Pleasanton, USA).
attributes.tag: ENA first public
attributes.value: 2026-02-28
attributes.tag: INSDC center name
attributes.value: Instiute of Pharmacology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Germany
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12700_2:Control_1
attributes.tag: age
attributes.value: 14
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: iAdipoQCre: wt, ATGL-flox: homo
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: 283076
attributes.tag: injury
attributes.value: ischemia reperfusion injury
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: heart
attributes.tag: sample name
attributes.value: E-MTAB-12700_2:Control_1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: B6.129-Pnpla2tm1Eek/J Tg(Adipoq-cre/ERT2)1Soff/J