title: Metagenome-assembled genome: ERP117304_co1_85
organism: uncultured Hyphomicrobiales bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3534981,ERR3534985 of study ERP117304.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: ERP117304_co1_85
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT2;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: engineered
attributes.tag: collection date
attributes.value: not provided
attributes.tag: completeness score
attributes.value: 86.9
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 2.37
attributes.tag: environmental medium
attributes.value: engineered
attributes.tag: geographic location (country and/or sea)
attributes.value: not provided
attributes.tag: geographic location (latitude)
attributes.value: not provided
attributes.tag: geographic location (longitude)
attributes.value: not provided
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: biofilm metagenome
attributes.tag: local environmental context
attributes.value: biofilm biome
attributes.tag: metagenomic source
attributes.value: biofilm metagenome
attributes.tag: project name
attributes.value: The establishment of epibacterial communities is fundamental to seaweed health, and fitness, in modulating ecological interactions and may also facilitate adaptation to new environments. Few recent studies have investigated the influence of abiotic factors like light, temperature as drivers of epibacterial community composition on seaweeds. Although salinity can determine bacterial abundance, growth and community composition, influence of salinity as a driver of epibacterial community composition has been rarely investigated for seaweeds and especially under long time scales. We also do not know how abiotic stressors may influence the 'core' bacterial species of seaweeds, if 'core' species exist. Following an initial (immediately after field collection, T0) sampling of epibacterial community of an invasive red seaweed Agarophyton vermicullophylum, we conducted a long term mesocosm experiment for 5 months, to examine the influence of three different salinities (low, medium and high) at two different time points (T1, T2) on the epibacterial community richness and composition of Agarophyton. Metagenomic sequencing showed that epibacterial communities changed significantly according to salinity and time points sampled. Epibacterial richness was significantly different between low and high salinities at both time points. Epibacterial richness also varied significantly among T1 and T2 within low, medium and high salinity level. Irrespective of salinity levels and time points sampled 727 taxa consistently appeared in all Agarophyton samples hinting at the presence of core bacterial species on the surface of the alga. Our results indicate that both salinity and time can be major driving forces in structuring epibacterial communities of seaweeds with respect to richness and β diversity. We highlight the necessity of conducting long term experiments allowing us to detect and understand epibacterial succession over time on seaweeds.
attributes.tag: sample derived from
attributes.value: SAMEA5789077,SAMEA5789081
attributes.tag: sample name
attributes.value: ERP117304_co1_85
attributes.tag: scientific_name
attributes.value: uncultured Hyphomicrobiales bacterium
attributes.tag: sequencing method
attributes.value: Illumina HiSeq 2500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach