title: Fol_13
organism: Gallus gallus
description: Protocols: At Day 28 of the feeding trial, 10 birds of each dietary group were mechanically stunned and slaughtered by decapitation. Tissue samples were collected from the right kidney, pancreas taken in anatomical proximity to the duodenal loop, uterus mucosa collected 2-5 cm upstream of the cloaca, and 5-10 follicles with an average diameter of 3 to 5 mm (large-white follicles). After dissection, the samples were rinsed with saline solution, snap frozen in liquid nitrogen and stored at -80 °C until use. RNA was extracted using TRIzol Reagent (Invitrogen, Karlsruhe, Germany), followed by DNase I digestion (Roche, Mannheim, Germany) and finally clean-up using the RNeasy Mini Spin kit (Qiagen). Total RNA of 2 µg was used for library preparation with the TruSeq Stranded mRNA Kit according to the manufacturer's recommendation (Illumina, San Diego, CA, USA).
attributes.tag: ENA first public
attributes.value: 2026-02-28
attributes.tag: INSDC center name
attributes.value: Research Institute for Farm Animal Biology (FBN) Physiological Genomics Unit
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14922:Fol_13
attributes.tag: age
attributes.value: 72
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: chicken
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: diet
attributes.value: Control diet
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: mature ovarian follicle
attributes.tag: sample name
attributes.value: E-MTAB-14922:Fol_13
attributes.tag: scientific_name
attributes.value: Gallus gallus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: Lohmann Brown