title: SW61_D14_MAN13p32_CF_U
organism: Homo sapiens
description: Protocols: Cells harvested into QIAGEN RLT buffer. Pellets homogenized with molecular grinding resin and plastic pestle. hESC/hiPSC cultured in TeSR™-E8™ on vitronectin. Differentiation to iMSCs via MesenPRO RS™. 3D pellets (200k cells) cultured in medium with TGFβ3 and BMP2. RNA extracted using QIAGEN RNeasy mini kit, as per manufacturers instructions. Library preparation of samples was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kits (Illumina).
attributes.tag: ENA first public
attributes.value: 2026-02-12
attributes.tag: INSDC center name
attributes.value: UOM-BCF
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16597:SW61_D14_MAN13p32_CF_U
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MAN13
attributes.tag: cell type
attributes.value: chondrocyte
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: Day 14 cartilage pellet
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: Cartilage
attributes.tag: sample name
attributes.value: E-MTAB-16597:SW61_D14_MAN13p32_CF_U
attributes.tag: scientific_name
attributes.value: Homo sapiens