title: Metagenome-assembled genome: binchicken_co179_98
organism: uncultured Candidatus Saccharibacteria bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR2586215,ERR2586216,ERR2586217 of study ERP108828.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: binchicken_co179_98
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT2;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: engineered
attributes.tag: collection date
attributes.value: 2017
attributes.tag: completeness score
attributes.value: 89.28
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 0.79
attributes.tag: environmental medium
attributes.value: engineered
attributes.tag: geographic location (country and/or sea)
attributes.value: Croatia
attributes.tag: geographic location (latitude)
attributes.value: 45.789725
attributes.tag: geographic location (longitude)
attributes.value: 16.08232
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: metagenome
attributes.tag: local environmental context
attributes.value: metagenome
attributes.tag: metagenomic source
attributes.value: metagenome
attributes.tag: project name
attributes.value: In this work, we have examined sludge samples from a Croatian azithromycin production plant using shotgun metagenomics, to better understand the full diversity of known resistance genes, mobile genetic elements and the impact on taxonomic composition caused by extensive antibiotic selection pressures. We compare this data to sludge from a wastewater treatment plant receiving municipal sewage. We find that the sludge from pharmaceutical production harbors around three times as much resistance genes as the municipal sewage sludge, with particularly large enrichments of aminoglycoside, amphenicol and sulfonamide resistance genes. The findings highlight that antibiotic production does contribute to the development of antibiotic resistance also in European settings and indicate a large potential for co-selection of resistance genes to a variety of antibiotic classes.
attributes.tag: sample derived from
attributes.value: SAMEA4668225,SAMEA4668226,SAMEA4668227
attributes.tag: sample name
attributes.value: binchicken_co179_98
attributes.tag: scientific_name
attributes.value: uncultured Candidatus Saccharibacteria bacterium
attributes.tag: sequencing method
attributes.value: Illumina HiSeq 2500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach