title: Control1-L
organism: uncultured organism
description: Protocols: Healthy hybrid groupers with an average body weight of 41.4 ± 2.9 g were selected for the experiment. All experimental fish were obtained from the Guangdong Provincial Marine Fisheries Research Institute. Before the experiment, fish were acclimated for 14 days in a temperature-controlled recirculating aquaculture system to ensure full adaptation to the laboratory environment. Throughout the acclimation and experimental periods, water temperature was strictly maintained between 25 and 28 °C. Salinity was regulated at approximately 12‰ by the addition of marine salt, pH was controlled within the range of 7.8-8.3, dissolved oxygen levels were kept above 6.0 mg/L, ammonia concentrations were maintained below 0.08 mg/L, and nitrite levels remained under 0.1 mg/L.  A commercial basal diet provided by Guangdong Yuequn Marine Biotechnology Co., Ltd. was used as the base feed. Oligochitosan (degree of deacetylation ≥ 90%) produced by Shandong Aokang Biotechnology Co., Ltd. was used as the dietary additive. Based on our previous findings (Chen et al. 2022, Shi et al. 2022), oligochitosan was supplemented at an optimal level of 800 mg/kg by weighing, mixing with the diet, and grinding into powder before feeding.  V. harveyi preserved in our laboratory was cultured on Luria-Bertani (LB) agar plates supplemented with ampicillin (prevent cross-contamination) at 28 °C for 24 h. The bacterial concentration was determined by measuring the optical density at 600 nm (OD₆₀₀) using a spectrophotometer. According to our previous study, the median lethal concentration (LD₅₀) of V. harveyi was determined to be 1.01 × 10⁶ CFU/g (Shi et al. 2022). The bacterial suspension was harvested at a concentration of 1.0 × 10⁷ CFU/mL and washed twice with PBS. The final inoculum was adjusted to 4.5 × 10⁴ CFU/g of fish, calculated according to the mean body weight.  Liver RNA from hybrid groupers was extracted using TRIzol reagent (Life Technologies, USA) following the manufacturer's procedure. cDNA libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit and sequenced on the Illumina HiSeq X Ten platform (OE Biotech Co., Ltd., Shanghai, China) (Ma et al. 2026).
attributes.tag: ENA first public
attributes.value: 2026-01-18
attributes.tag: INSDC center name
attributes.value: Zhongkai University of Agriculture and Engineering， Guangzhou, Guangdong Province, China.
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16424:Control1-L
attributes.tag: age
attributes.value: 0.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: developmental stage
attributes.value: fine
attributes.tag: genotype
attributes.value: none
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: same
attributes.tag: infect
attributes.value: no
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-16424:Control1-L
attributes.tag: scientific_name
attributes.value: uncultured organism
attributes.tag: sex
attributes.value: male
attributes.tag: stimulus
attributes.value: Control
attributes.tag: strain
attributes.value: Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂