title: Metagenome-assembled genome: binchicken_co77_115
organism: uncultured Thermotogae bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR12088346,SRR12088347 of study SRP268746.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: binchicken_co77_115
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: aquatic
attributes.tag: collection date
attributes.value: 2019-07-10
attributes.tag: completeness score
attributes.value: 84.44
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 0.78
attributes.tag: environmental medium
attributes.value: aquatic
attributes.tag: geographic location (country and/or sea)
attributes.value: Spain
attributes.tag: geographic location (latitude)
attributes.value: 40.546053
attributes.tag: geographic location (longitude)
attributes.value: -2.050427
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: lake water metagenome
attributes.tag: local environmental context
attributes.value: lake water biome
attributes.tag: metagenomic source
attributes.value: lake water metagenome
attributes.tag: project name
attributes.value: The case study is focused on the crenogenic meromictic Lake El Tobar (Spain), a continental karstified system formed by the sink and solution of a combination of Cretaceous dolomites and Triassic salt-rich Keuper. One of the peculiarities of this system is the formation of a salinity gradient from 0.6 to > 200 mS cm-1, that generates an anoxic saline monimolimnion starting at 13-14 m and reaching the bottom at 18 m of depth. Samples were obtained from 2, 12, 12.5, 13, 13.5, 14, integrated 15.5-16.5 and 18 m of depth to study the microbial vertical zoning and stratification patterns of the lake. Water samples were filtered through a series of 20, 5 and 0.22 micron filters. The 0.22 micron filters were sequenced with high-throughput Shotgun sequencing. We provide 8 metagenomic datasets and MAGs derived from these samples.
attributes.tag: sample derived from
attributes.value: SAMN15249361,SAMN15249360
attributes.tag: sample name
attributes.value: binchicken_co77_115
attributes.tag: scientific_name
attributes.value: uncultured Thermotogae bacterium
attributes.tag: sequencing method
attributes.value: HiSeq X Ten
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach