title: Metagenome-assembled genome: binchicken_co358_31
organism: uncultured Proteiniphilum sp.
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR3901708,SRR5272663 of study SRP056932.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: binchicken_co358_31
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT2;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: engineered
attributes.tag: collection date
attributes.value: not provided
attributes.tag: completeness score
attributes.value: 99.93
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 5.23
attributes.tag: environmental medium
attributes.value: engineered
attributes.tag: geographic location (country and/or sea)
attributes.value: South Africa
attributes.tag: geographic location (latitude)
attributes.value: not provided
attributes.tag: geographic location (longitude)
attributes.value: not provided
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: bioreactor metagenome
attributes.tag: local environmental context
attributes.value: bioreactor biome
attributes.tag: metagenomic source
attributes.value: bioreactor metagenome
attributes.tag: project name
attributes.value: Gold mining and other industrial activities make extensive use of cyanide (CN-) and produce large volumes of thiocyanate- (SCN-) contaminated wastewater that must be treated before reuse in mining processes. Furthermore, contaminated wastewater stored in tailings dams may pose an environmental threat through the potential for leakage into local groundwater. Microbial communities are often used for biodegradation and environmental remediation, however, little is known about the membership and metabolic potential of SCN-degrading consortia. Application and improvement of the remediation strategy will benefit from an understanding of organisms that can contribute to the breakdown of SCN- into sulfur, carbon and nitrogen compounds. To this end, we performed metagenomic analysis of samples from two laboratory-scale bioreactors used to study SCN- and CN- degradation by established microbial consortia. Community analysis revealed the dominance of Thiobacillus spp. whose genomes harbor a previously unreported operon for SCN- degradation. Metabolic predictions suggest that a large portion of each bioreactor community is autotrophic, relying not on molasses in reactor feed, but using energy gained from oxidation of the sulfur compounds produced during SCN- degradation. Heterotrophs compose a smaller portion of the reactor bacterial community, and predation by phage and eukaryotes is predicted to affect community dynamics. Furthermore, genes for ammonium oxidation and denitrification were detected, possibly allowing removal of nitrogen as required for complete remediation of wastewater. These findings suggest possible optimization strategies for reactor design such as improved aerobic/anaerobic partitioning and elimination of organic carbon from reactor feed.
attributes.tag: sample derived from
attributes.value: SAMN05389823,SAMN06267629
attributes.tag: sample name
attributes.value: binchicken_co358_31
attributes.tag: scientific_name
attributes.value: uncultured Proteiniphilum sp.
attributes.tag: sequencing method
attributes.value: Illumina HiSeq 2500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach