title: dotA4
organism: Homo sapiens
description: Protocols: THP-1 cells were differentiated using PMA (50 ng/mL) for 3 days, then infected with either WT or mutant Lp 130b strains for 18h at MOI 10.  RNA was extracted using the IsolateII RNA Mini kit (Bioline) according to manufacturer's instruction. After QC using Bioanalyzer, only samples with RIN >8.0 were used. The cDNA libraries for RNA sequencing were generated from 20 ng of RNA using an in-house multiplex RNA-seq method, where sample-specific indices were incorporated during oligo-d(T) priming of first strand cDNA (MHTP Medical Genomics Facility, https://doi.org/10.1038/s41467-021-23111-1).
attributes.tag: ENA first public
attributes.value: 2026-02-28
attributes.tag: INSDC center name
attributes.value: Hudson Institute of Medical Research
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16655:dotA4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: THP-1
attributes.tag: cell type
attributes.value: macrophage
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: Legionnaires' disease
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-16655:dotA4
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: stimulus
attributes.value: Delta-dotA Lp 130b infection, 18h, repeat 3