title: Metagenome-assembled genome: SRP200020_co5_4
organism: uncultured Gammaproteobacteria bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR9166044,SRR9166051 of study SRP200020.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: SRP200020_co5_4
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT2;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: aquatic
attributes.tag: collection date
attributes.value: 2014-11-01
attributes.tag: completeness score
attributes.value: 94.52
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 0.01
attributes.tag: environmental medium
attributes.value: aquatic
attributes.tag: geographic location (country and/or sea)
attributes.value: Venezuela
attributes.tag: geographic location (latitude)
attributes.value: 10.617
attributes.tag: geographic location (longitude)
attributes.value: -65.167
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: seawater metagenome
attributes.tag: local environmental context
attributes.value: seawater biome
attributes.tag: metagenomic source
attributes.value: seawater metagenome
attributes.tag: project name
attributes.value: A persistent chemoautotrophy maximum is present in many anoxic basins at the sulfidic layer's upper boundary, but the microbiology and biogeochemistry that control this feature are poorly understood. In 13 of 31 cruises to the Cariaco Basin the particulate organic carbon pool was enriched in 13C (δ13CPOC as high as -16‰) within the oxic/sulfidic transitional layer compared to photic zone values (-23 to -26‰) and 13C enrichments persisted to depth. During these “heavy” cruises, dissolved O2 and [NO3- + NO2-] concentrations and their computed fluxes to the oxic/sulfidic interface were significantly lower than during “light” cruises. Cruises with isotopically heavy POC were more common between 2013 and 2015 when suspended particles below the photic zone tended to be nitrogen-rich compared to later cruises. Within the chemoautotrophic layer, nitrogen-rich particles (POC/PN< 10) were more likely to be 13C-enriched than nitrogen-poor particles among all cruises, implying these samples were dominated by living cells and fresh detritus rather than laterally transported or extensively decomposed biogenic debris. Dissolved inorganic carbon (DIC) assimilation during “heavy” cruises (n = 3) was faster and occurred deeper than during “light” cruises (n = 2). Metagenomics data from the chemoautotrophic layer during two cruises support prevalence of microorganisms carrying RuBisCO form II genes, which encode a carbon fixation enzyme that discriminates less against heavy isotopes than most other carbon fixation enzymes. Metatranscriptomics data confirm that form II RuBisCO genes were more highly expressed during both cruises and at depths where essential reactants coexist.
attributes.tag: sample derived from
attributes.value: SAMN11854482,SAMN11854485
attributes.tag: sample name
attributes.value: SRP200020_co5_4
attributes.tag: scientific_name
attributes.value: uncultured Gammaproteobacteria bacterium
attributes.tag: sequencing method
attributes.value: NextSeq 500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach