title: A008
organism: Mus musculus
description: Protocols: ISCs from 6 PBS and 6 dm-aKG treated mice were sorted  total DNA was isolated using Quick-DNA microprep kit(Zymo). DNA was fractionated using Bioruptor NGS(Diagenode)  after adding control DNAs from the NEBNext Enzymatic 5hmC-seq Kit(NEB-E3350S) to 250 ng of sample. The sonicated DNA libraries were constructed using NEBNext Primers for Epigenetics(Dual Index Primer Pairs Set 2B)(NEB-E3392S).
attributes.tag: ENA first public
attributes.value: 2025-05-24
attributes.tag: INSDC center name
attributes.value: Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland Molecular and Integrative Bioscience Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15115:A008
attributes.tag: age
attributes.value: 5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Lgr5-EGFP-IRES-CreERT2(+/-)
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: PK6351A
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: small intestine
attributes.tag: sample name
attributes.value: E-MTAB-15115:A008
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: stimulus
attributes.value: dm-aKG
attributes.tag: strain
attributes.value: C57BL/6J