title: Sample 2
organism: Mus musculus
description: Protocols: Immortalized MEF cells were washed once with ice-cold PBS 10ug/ml cyclohexamide.  Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cyclohexamide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028).  Immagina Biotechnology LACEseq #LS-001_12
attributes.tag: ENA first public
attributes.value: 2025-06-10
attributes.tag: INSDC center name
attributes.value: Istituto Nazionale di Genetica Molecolare (INGM)
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14299:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: MEF cell line
attributes.tag: cell_type
attributes.value: mouse embryonic fibroblast cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: embryonic tissue
attributes.tag: sample name
attributes.value: E-MTAB-14299:Sample 2
attributes.tag: scientific_name
attributes.value: Mus musculus