title: Metagenome-assembled genome: binchicken_co104_59
organism: uncultured Oscillospiraceae bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs DRR182650,DRR182651 of study DRP005178.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: binchicken_co104_59
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT2;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: engineered
attributes.tag: collection date
attributes.value: 2018
attributes.tag: completeness score
attributes.value: 86.76
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 1.07
attributes.tag: environmental medium
attributes.value: engineered
attributes.tag: geographic location (country and/or sea)
attributes.value: Japan
attributes.tag: geographic location (latitude)
attributes.value: 35.6693
attributes.tag: geographic location (longitude)
attributes.value: 139.4257
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: sludge metagenome
attributes.tag: local environmental context
attributes.value: sludge biome
attributes.tag: metagenomic source
attributes.value: sludge metagenome
attributes.tag: project name
attributes.value: Conductive nano-materials have been reported to accelerate methanogenesis by promoting direct interspecies electron transfer (DIET), while their effects seem to vary depending on operational conditions. The present study examined effects of magnetite nanoparticles (MNPs) on methanogenesis from acetate by soil-derived anaerobic cultures under continuous agitation. We found that MNPs accelerated methanogenesis in agitated cultures as has been observed previously for static cultures. Metabarcoding of 16S rRNA gene amplicons showed that Methanosarcina substantially increased in the presence of MNPs, while DIET-related Geobacter did not occur. Metagenomic and metatranscriptomic analyses confirmed the predominance of Methanosarcina in MPN-supplemented cultures. In addition, genes coding for acetoclastic methanogenesis but not those for hydrogenotrophic methanogenesis were abundantly expressed in the dominant Methanosarcina in the presence of MNPs. These results suggest that MNPs stimulate acetoclastic methanogenesis under continuous agitation.
attributes.tag: sample derived from
attributes.value: SAMD00175448,SAMD00175449
attributes.tag: sample name
attributes.value: binchicken_co104_59
attributes.tag: scientific_name
attributes.value: uncultured Oscillospiraceae bacterium
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach