title: H1299-shAASS-03
organism: Homo sapiens
description: Protocols: Cells were collected by Trypsization and washed with PBS twice.  All cells were cultured in RPMI medium supplemented with 10% FBS and 1% penicillin–streptomycin. After cell collection, total RNA was extracted using the RNeasy Kit (QIAGEN) according to the manufacturer's instructions. Approximately 2 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). Following purification, the poly(A) mRNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with a strand-specific library preparation by dUTP method. The average insert size for the paired-end libraries was 300±50 bp.
attributes.tag: ENA first public
attributes.value: 2026-02-28
attributes.tag: INSDC center name
attributes.value: Emory University
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16032:H1299-shAASS-03
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: H1299
attributes.tag: cell type
attributes.value: lung cancer cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: cancer
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-16032:H1299-shAASS-03
attributes.tag: scientific_name
attributes.value: Homo sapiens