title: HD_F_GON12944918
organism: Homo sapiens
description: Protocols: We used the previous protocol optimised for gonadal dissociation and available at protocols.io136. In short, tissues were cut into <1 mm3 segments before being digested with a mix of Trypsin-EDTA 0.25% and DNaseI (0.1mg/ml) for 5-15 minutes at 37°C with intermittent shaking. Samples > 17 PCW were digested using a combination of collagenase and Trypsin/EDTA, a protocol adapted from Wagner et al.,136,137. In short, samples were first digested with a mix of Collagenase 1A (1 mg/ml), DNaseI (0.1 mg/ml) and Liberase TM (50 µg/ml) for 45 minutes at 37°C with rotation. Cell solution was further digested with Trypsin/EDTA 0.25% for 10 minutes at 37°C with rotation. In both protocols, digested tissue was passed through a 100 µm filter, and cells collected by centrifugation (500 x g for 5 minutes at 4°C). Cells were washed and resuspended in PBS-BSA 0.04% prior to cell counting.  For the scRNA-seq experiments, cells were loaded according to the manufacturer's protocol for the Chromium Next GEM Single Cell 5′ v2 (DUAL) Kit  (10X Genomics) to attain between 2,000 and 10,000 cells per reaction. Library preparation was carried out according to the manufacturer's protocol.
attributes.tag: ENA first public
attributes.value: 2025-08-07
attributes.tag: INSDC center name
attributes.value: Wellcome Sanger Institute, European Bioinformatics Institute
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15475:HD_F_GON12944918
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: 20 PCW, 12 PCW, 12 PCW
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: Hrv161, C130, C132
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: external genitalia; full reproductive tract; external genitalia
attributes.tag: sample name
attributes.value: E-MTAB-15475:HD_F_GON12944918
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male, male, male