title: StiffHydrogel_4T1_RNAseq_rep1
organism: Mus musculus
description: Protocols: 4T1 breast cancer cells were cultured on soft (1.24±1.3kPa ) and stiff (13.20±2.3 kPa) two-dimensional polyacrylamide hydrogels for 24hrs before being harvested for RNA extraction 4T1 breast cancer cells were cultured on stiff (13.20±2.3kPa ) two-dimensional polyacrylamide hydrogels for 24hrs RNA extraction was performed using the Qiagen RNAeasy extraction kit as per manufacturers recommendations The quality of the RNA was confirmed using TapeStation (Agilent), prior to library preparation using the KAPA mRNA HyperPrep Kit, as per the manufacturer protocol. Quality control was performed on the mRNA library using a LabChip GX prior to RNA sequencing.
attributes.tag: ENA first public
attributes.value: 2024-01-01
attributes.tag: External Id
attributes.value: SAMEA13204334
attributes.tag: INSDC center name
attributes.value: Garvan Institute of Medical Research and The Kinghorn Cancer Center, Darlinghurst, NSW, Australia
attributes.tag: INSDC last update
attributes.value: 2022-03-01T17:51:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11487:StiffHydrogel_4T1_RNAseq_rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: 4T1
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: breast carcinoma
attributes.tag: growth condition
attributes.value: cultured on stiff (13.20±2.3kPa) two-dimensional polyacrylamide hydrogels for 24 hours
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: mammary gland
attributes.tag: sample name
attributes.value: E-MTAB-11487:StiffHydrogel_4T1_RNAseq_rep1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: BALB/c