title: Metagenome-assembled genome: SRP284006_co1_553
organism: uncultured Verrucomicrobiota bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs SRR12676969,SRR12676970 of study SRP284006.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: SRP284006_co1_553
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: VAMB;3.0.2;default
attributes.tag: broad-scale environmental context
attributes.value: aquatic
attributes.tag: collection date
attributes.value: 2017-09-10
attributes.tag: completeness score
attributes.value: 80.89
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 0.88
attributes.tag: environmental medium
attributes.value: aquatic
attributes.tag: geographic location (country and/or sea)
attributes.value: Canada
attributes.tag: geographic location (latitude)
attributes.value: 49.69
attributes.tag: geographic location (longitude)
attributes.value: -93.69
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: freshwater metagenome
attributes.tag: local environmental context
attributes.value: freshwater biome
attributes.tag: metagenomic source
attributes.value: freshwater metagenome
attributes.tag: project name
attributes.value: Boreal Shield lakes number in the millions globally, and an estimated 15% of these lakes develop anoxic waters seasonally. These anoxic waters commonly become ferruginous, with high levels of dissolved ferrous iron and low levels of sulfate and sulfide. Such ferruginous ecosystems have been poorly explored in a modern environmental context and are also relevant toward understanding early Earth ecosystems such as the anoxic and ferruginous oceans predicted during the Archaean Eon (approx. 3.8-2.5 billion years ago). Here we surveyed multiple Boreal Shield lakes at the International Institute for Sustainable Development Experimental Lakes Area (IISD-ELA near Kenora, Ontario, Canada), sampling a core set of eight seasonally anoxic lakes, a permanently oxic reference lake, and samples from other lakes obtained where possible. In this BioProject, metagenome, metatranscriptome, and 16S ribosomal RNA gene amplicon sequencing data are deposited for the 2017-2018 portion of the survey. Data from the 2016 portion of the survey are available under the Joint Genome Institute (JGI) Genome Portal under Proposal ID 502896. Note that this BioProject also contains a small number of 2016 samples (amplicon data only) that were re-sequenced due to failed sequencing in the 2016 run.
attributes.tag: sample derived from
attributes.value: SAMN16221733,SAMN16221732
attributes.tag: sample name
attributes.value: SRP284006_co1_553
attributes.tag: scientific_name
attributes.value: uncultured Verrucomicrobiota bacterium
attributes.tag: sequencing method
attributes.value: Illumina HiSeq 2500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach