title: Metagenome-assembled genome: binchicken_co280_143
organism: uncultured Vitreoscilla sp.
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3212003,ERR4678565 of study ERP114226.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: binchicken_co280_143
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: aquatic
attributes.tag: collection date
attributes.value: not provided
attributes.tag: completeness score
attributes.value: 73.87
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 1.02
attributes.tag: environmental medium
attributes.value: aquatic
attributes.tag: geographic location (country and/or sea)
attributes.value: not applicable
attributes.tag: geographic location (latitude)
attributes.value: not provided
attributes.tag: geographic location (longitude)
attributes.value: not provided
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: wastewater metagenome
attributes.tag: local environmental context
attributes.value: wastewater biome
attributes.tag: metagenomic source
attributes.value: wastewater metagenome
attributes.tag: project name
attributes.value: Based on 4 samples processed differently (Pig feces 1 & 2 (P1 & P2) Sewage 1 & 2 (S1 & S2)), storage conditions (Time 0h, 16h, 64h Temperature (-80C, -20C, 5C, 22C)), sequencing platform (FTX=Freeze thawing, HX=Nextflex Hiseq, LTSX=Kappa Hiseq, NX1NS=Nextera Nextseq, NX2NS=Nextera Nextseq, NFNS=Nextflex Nextseq). Sample names containing HHKTMBBXX are resequenced samples. Sample names containing MC are spiked with a mock community. Sample names containing EC, ECPBS and pureMC, are negative DNA extraction controls, negative controls containing PBS and postive controls of the mock community used to spike with, respectively. The a, b and c are indicators of replicate number.
attributes.tag: sample derived from
attributes.value: SAMEA5414648,SAMEA7426265
attributes.tag: sample name
attributes.value: binchicken_co280_143
attributes.tag: scientific_name
attributes.value: uncultured Vitreoscilla sp.
attributes.tag: sequencing method
attributes.value: NextSeq 500,Illumina NovaSeq 6000
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach