title: Metagenome-assembled genome: ERP119705_co4_85
organism: uncultured Thermodesulfobacteriota bacterium
description: This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic runs ERR3858111,ERR3858129 of study ERP119705.
attributes.tag: ENA first public
attributes.value: 2024-12-11
attributes.tag: INSDC center name
attributes.value: CMR
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: ERP119705_co4_85
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes;3.15.4;default
attributes.tag: binning parameters
attributes.value: coverage and kmer
attributes.tag: binning software
attributes.value: MetaBAT2;2.15;default
attributes.tag: broad-scale environmental context
attributes.value: aquatic
attributes.tag: collection date
attributes.value: 2019-01-07
attributes.tag: completeness score
attributes.value: 73.34
attributes.tag: completeness software
attributes.value: CheckM2;1.0.2;default
attributes.tag: contamination score
attributes.value: 2.23
attributes.tag: environmental medium
attributes.value: aquatic
attributes.tag: geographic location (country and/or sea)
attributes.value: not provided
attributes.tag: geographic location (latitude)
attributes.value: not provided
attributes.tag: geographic location (longitude)
attributes.value: not provided
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: groundwater metagenome
attributes.tag: local environmental context
attributes.value: groundwater biome
attributes.tag: metagenomic source
attributes.value: groundwater metagenome
attributes.tag: project name
attributes.value: The CRC AquaDiva (www.aquadiva.uni-jena.de) focuses on the important roles of water (Aqua) and biodiversity (Diva) for shaping the structure, properties, and functions of the subsurface, the part of the Earth's Critical Zone (CZ) that begins below the highest density of plant roots and extends down into the first aquifers. In this study, we generated short-read sequencing data for 32 groundwater samples from a well-characterized monitoring transect of the Hanich Critical Zone Exploratory (NW Thuringia, Germany) that encompasses 15 monitoring wells spread across a hillslope. Groundwater was collected from five wells (namely H14, H32, H41, H43, H51, H52) and was sequentially filtered through 0.2 uM, and 0.1 uM PTFE filters (142 mm, Omnipore Membrane, Merck Millipore, Germany). Filters were immediately frozen on dry ice and transported to a -80 C freezer. Library preparation and sequencing were performed with the help of the Core Facility DNA Sequencing at the Leibniz Institute on Aging (FLI, Jena, Germany). Briefly, libraries for all 32 samples were prepared using the NEB Next Ultra II DNA kit and multiplexed on four lanes on a NextSeq500 using 300 cycles for sequncing yielding 150 bp paired-end reads.
attributes.tag: sample derived from
attributes.value: SAMEA6512631,SAMEA6512632
attributes.tag: sample name
attributes.value: ERP119705_co4_85
attributes.tag: scientific_name
attributes.value: uncultured Thermodesulfobacteriota bacterium
attributes.tag: sequencing method
attributes.value: NextSeq 500
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach