organism: mixed culture metagenome
attributes.tag: ENA first public
attributes.value: 2025-02-12
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF SZEGED
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: BACMIX_PRMT_GP2_GMS_PB_20240807_bc38
attributes.tag: adapters
attributes.value: Top strand 5'-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3'; Bottom strand 5'-ACGTAACTGAACGAAGTACAGG-3'
attributes.tag: broad-scale environmental context
attributes.value: Csongrad county
attributes.tag: collection date
attributes.value: 2024-08-04
attributes.tag: environmental medium
attributes.value: ZymoBIOMICS Gut Microbiome Standard
attributes.tag: geographic location (country and/or sea)
attributes.value: Hungary
attributes.tag: geographic location (latitude)
attributes.value: 46.248755
attributes.tag: geographic location (longitude)
attributes.value: 20.145766
attributes.tag: library construction method
attributes.value: 16S barcoding (SQK-16S024)/ Native Barcoding Kit 94 V14 (SQK-LSK114.96)
attributes.tag: library screening strategy
attributes.value: ligation based
attributes.tag: local environmental context
attributes.value: Szeged
attributes.tag: multiplex identifiers
attributes.value: ACCACAGGAGGACGATACAGAGAA/TTCTCTGTATCGTCCTCCTGTGGT
attributes.tag: nucleic acid amplification
attributes.value: LongAmp HotStart Taq 2X Master Mix
attributes.tag: nucleic acid extraction
attributes.value: ZymoBIOMICS 96 MagBead DNA Kit
attributes.tag: pcr conditions
attributes.value: Initial denaturation 1 min 95 �C (1 cycle); Denaturation 20 secs 95 �C (25 cycles); Annealing 30 secs 55 �C (25 cycles); Extension 2 mins 65 �C (25 cycles); Final extension 5 mins 65 �C (1 cycle)
attributes.tag: pcr primers
attributes.value: 27F 5'-(AGRGTTYGATYMTGGCTCAG); 1492R 5'-(RGYTACCTTGTTACGACTT)
attributes.tag: project name
attributes.value: Evaluating Primer Bias in Full-Length 16S Sequencing of Synthetic and Host Fecal Samples
attributes.tag: sample name
attributes.value: BACMIX_PRMT_GP2_GMS_PB_20240807_bc38
attributes.tag: scientific_name
attributes.value: mixed culture metagenome
attributes.tag: sequence quality check
attributes.value: software
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V1-V9