organism: Canis lupus familiaris
attributes.tag: ENA first public
attributes.value: 2025-02-12
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF SZEGED
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Canfam_SRTP_A_M2_210415_ZHMW
attributes.tag: adapters
attributes.value: 5�-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3�
attributes.tag: biological status
attributes.value: breeder's line
attributes.tag: broad-scale environmental context
attributes.value: Danube Basin
attributes.tag: collection date
attributes.value: 2021-04-15
attributes.tag: common name
attributes.value: dog
attributes.tag: environmental medium
attributes.value: no
attributes.tag: geographic location (country and/or sea)
attributes.value: Hungary
attributes.tag: geographic location (latitude)
attributes.value: 47.31955
attributes.tag: geographic location (longitude)
attributes.value: 19.324268
attributes.tag: geographic location (region and locality)
attributes.value: D�ny
attributes.tag: gravidity
attributes.value: NA
attributes.tag: host age
attributes.value: 8
attributes.tag: host body product
attributes.value: stool
attributes.tag: host common name
attributes.value: Hungarian herding terrier: Pumi
attributes.tag: host diet
attributes.value: Gemon adult lamb Rice/Gemon mini starter salmon 3:1
attributes.tag: host family relationship
attributes.value: No relationship, same living environment
attributes.tag: host life stage
attributes.value: adult
attributes.tag: host scientific name
attributes.value: Canis lupus familiaris
attributes.tag: host sex
attributes.value: male
attributes.tag: host subject id
attributes.value: SRTP_A_M2
attributes.tag: host subspecific genetic lineage
attributes.value: cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
attributes.tag: host taxid
attributes.value: 9615
attributes.tag: library construction method
attributes.value: Rapid sequencing amplicons - 16S barcoding (SQK-RAB204)
attributes.tag: library screening strategy
attributes.value: AMPLICON
attributes.tag: library size
attributes.value: 1500
attributes.tag: local environmental context
attributes.value: Garden
attributes.tag: multiplex identifiers
attributes.value: GAGTCTTGTGTCCCAGTTACCAGG
attributes.tag: nucleic acid amplification
attributes.value: LongAmp Taq 2X Master Mix
attributes.tag: nucleic acid extraction
attributes.value: Zymo Quick-DNA HMW MagBead Kit
attributes.tag: pcr conditions
attributes.value: Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
attributes.tag: pcr primers
attributes.value: 16SFw 5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'; 16S Rev 5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'
attributes.tag: project name
attributes.value: Metagenomic sequencing for longitudinal analysis of dog gut microbiome
attributes.tag: sample name
attributes.value: Canfam_SRTP_A_M2_210415_ZHMW
attributes.tag: sample storage location
attributes.value: Eppendorf_CryoCube_F740h
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 0,1
attributes.tag: scientific_name
attributes.value: Canis lupus familiaris
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V1-V9
attributes.tag: sub_species
attributes.value: familiaris