organism: Canis lupus familiaris
attributes.tag: ENA first public
attributes.value: 2025-02-12
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF SZEGED
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Canfam_PRMT_LM2_canis_MONT
attributes.tag: broad-scale environmental context
attributes.value: Csongrad-Csanad county
attributes.tag: collection date
attributes.value: 2023-03-25
attributes.tag: common name
attributes.value: dog
attributes.tag: environmental medium
attributes.value: no
attributes.tag: geographic location (country and/or sea)
attributes.value: Hungary
attributes.tag: geographic location (latitude)
attributes.value: 46.248755
attributes.tag: geographic location (longitude)
attributes.value: 20.145766
attributes.tag: host age
attributes.value: 2.8
attributes.tag: host sex
attributes.value: female
attributes.tag: library construction method
attributes.value: 16S barcoding (SQK-16S024)/ Native Barcoding Kit 94 V14 (SQK-LSK114.96)
attributes.tag: local environmental context
attributes.value: Szeged
attributes.tag: multiplex identifiers
attributes.value: ACCCTCCAGGAAAGTACCTCTGAT/ATCAGAGGTACTTTCCTGGAGGGT
attributes.tag: nucleic acid extraction
attributes.value: ZymoBIOMICS 96 MagBead DNA Kit
attributes.tag: pcr primers
attributes.value: 27F 5'-(AGRGTTYGATYMTGGCTCAG); 1492R 5'-(CGGYTACCTTGTTACGACTT)
attributes.tag: project name
attributes.value: Evaluating Primer Bias in Full-Length 16S Sequencing of Synthetic and Host Fecal Samples
attributes.tag: sample name
attributes.value: Canfam_PRMT_LM2_canis_MONT
attributes.tag: sample storage duration
attributes.value: 501
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: Canis lupus familiaris
attributes.tag: sequence quality check
attributes.value: software
attributes.tag: sequencing method
attributes.value: Oxford Nanopore
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V1-V9
attributes.tag: temperature
attributes.value: 11
attributes.tag: sub_species
attributes.value: familiaris