organism: Fusarium oxysporum
attributes.tag: isolate
attributes.value: PkF01_WT_2
attributes.tag: collection_date
attributes.value: 2019-11
attributes.tag: env_broad_scale
attributes.value: ENVO:01001021
attributes.tag: env_local_scale
attributes.value: ENVO:01001540
attributes.tag: env_medium
attributes.value: ENVO:06100015
attributes.tag: geo_loc_name
attributes.value: China:Yunnan,Baoshan
attributes.tag: host
attributes.value: Polygonatum kingianum
attributes.tag: isol_growth_condt
attributes.value: The fungus was isolated from a diseased rhizome of Polygonatum kingianum (Yunnan goldenseal) exhibiting typical root rot symptoms. The tissue was surface-sterilized with 75% ethanol for 30 seconds and 1% sodium hypochlorite for 90 seconds, followed by three rinses in sterile distilled water. After drying, small segments of internal tissue were aseptically placed on Potato Dextrose Agar (PDA) plates. Plates were incubated at 25°C in the dark. A pure culture was obtained by transferring hyphal tips from the colony margin to fresh PDA plates. The isolate is maintained on PDA slants at 25°C and long-term stored as spore suspensions in 20% glycerol at -80°C.
attributes.tag: lat_lon
attributes.value: 25.1121 N 99.1618 E
attributes.tag: BioSampleModel
attributes.value: MIGS.eu
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.plant-associated