organism: metagenome
attributes.tag: host
attributes.value: Soil
attributes.tag: isolation_source
attributes.value: Inoculation artificial medium with soil sample
attributes.tag: collection_date
attributes.value: 2022-05-17
attributes.tag: geo_loc_name
attributes.value: Poland: Lubelskie Voivodeship
attributes.tag: lat_lon
attributes.value: 51.04815480 N 22.23760263 E
attributes.tag: rel_to_oxygen
attributes.value: aerobe
attributes.tag: samp_mat_process
attributes.value: Microbial genomic DNA was extracted from enriched cultures in triplicate, using a PowerLyzer PowerSoil DNA Isolation Kit (Qiagen, Hilden, Germany). The V4 region of the 16S rRNA gene amplicons were sequenced using the universal primers 515F (5-GTGYCAGCMGCCGCGGTAA) and 806R (5-GGACTACNVGGGTWTCTAAT).
attributes.tag: samp_size
attributes.value: 0.2 mL
attributes.tag: library_id
attributes.value: H5SG02102
attributes.tag: title
attributes.value: Metataxonomy of Soil microbial community of Leptosol exposed to 2ug/mL glyphosate and 10ug/mL of nonanoic acid.
attributes.tag: library_strategy
attributes.value: AMPLICON
attributes.tag: library_source
attributes.value: METAGENOMIC
attributes.tag: library_selection
attributes.value: PCR
attributes.tag: library_layout
attributes.value: paired
attributes.tag: platform
attributes.value: ILLUMINA
attributes.tag: instrument_model
attributes.value: Illumina NovaSeq 6000
attributes.tag: design_description
attributes.value: Microbial genomic DNA was extracted from soil samples in duplicate using a PowerLyser Power Soil (Qiagen, Hilden, Germany), according to the manufacturers recommendations. Microbial community structure was analyzed using the V4 region of the 16S rRNA gene amplicon, which were sequenced with Illumina technology using universal primers 515F (5-GTGYCAGCMGCCGCGGTAA) and 806R (5-GGACTACNVGGGTWTCTAAT). Sequence libraries were generated using NEB Next Ultra II FS DNA PCR- free LibraryPrep Kit with an Illumina NovaSeq6000 platform.
attributes.tag: filetype
attributes.value: fastq
attributes.tag: filename
attributes.value: H5SG02102.raw_1.fastq
attributes.tag: filename2
attributes.value: H5SG02102.raw_2.fastq
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental