title: smRNA reads from Montipora capitata collected at 22 hour post-fertilization; Zymo libraries
description: Egg-sperm bundles from Montipora capitata were collected in 50 mL falcon tubes from Reef 13 (21°27'05"N 157°47'45"W) on June 17, 2023 at 21:45 HST. Bundles were brought back to the Hawai'i Institute of Marine Biology and left to fertilize for 1 hour. Falcon tubes were gently rocked every 5 minutes to ensure bundles were broken up. Following fertilization, sperm water was removed and eggs were rinsed 3x with 0.2 µm filtered seawater (FSW). Sample was collected at mid gastrula stage (22 hpf).
attributes.tag: isolate
attributes.value: Coral host
attributes.tag: breed
attributes.value: Not applicable
attributes.tag: host
attributes.value: Not applicable
attributes.tag: isolation_source
attributes.value: Not applicable
attributes.tag: collection_date
attributes.value: 2023-06-18
attributes.tag: geo_loc_name
attributes.value: USA: Kaneohe Bay Oahu Hawaii
attributes.tag: tissue
attributes.value: Whole organism
attributes.tag: collected_by
attributes.value: Jill Ashey
attributes.tag: dev_stage
attributes.value: Mid gastrula
attributes.tag: env_broad_scale
attributes.value: coral reef [ENVO:00000150]
attributes.tag: host_tissue_sampled
attributes.value: Whole host organism
attributes.tag: identified_by
attributes.value: Lamarck
attributes.tag: lat_lon
attributes.value: 21.421721 N 157.799744 W
attributes.tag: Replicate
attributes.value: 2
attributes.tag: Grants
attributes.value: Graduate Research Fellowship to JA, GRFP INTERN supplement 1921465 to HMP, OCE PRF 2205966 to ASH
attributes.tag: PermitNumber
attributes.value: SAP 2024-24 (Putnam)
attributes.tag: BioSampleModel
attributes.value: Invertebrate