title: IL22 high
description: FASTQ from IL-22 High.
attributes.tag: strain
attributes.value: NIH Swiss
attributes.tag: age
attributes.value: NA
attributes.tag: collection_date
attributes.value: NA
attributes.tag: geo_loc_name
attributes.value: USA
attributes.tag: sex
attributes.value: NA
attributes.tag: tissue
attributes.value: NA
attributes.tag: cell_line
attributes.value: MNK-3
attributes.tag: cell_subtype
attributes.value: MNK3i
attributes.tag: sample_type
attributes.value: Cell Culture
attributes.tag: treatment
attributes.value: MNK3i cells were transduced with a CRISPRi lentiviral gRNA library targeting 20K genes, selected for guide integration with puromycin (2ug/ml), treated with doxycycline (2ug/ml) for 48hr to induce dCas9-KRAB expression, and then stimulated with IL-1b and IL-23 (10ng/ml each) for 21hr. Cells were stained for intracellular IL-22 and sorted by flow cytometry into IL-22 negative, low, or high populations. This is IL-22 high sample.
attributes.tag: BioSampleModel
attributes.value: Model organism or animal