title: Saccharomyces cerevisiae IMZ616
description: The maltose negative S. cerevisiae strain IMZ616 originates from S. cerevisiae CEN.PK102-3A, which carries three MAL loci (MAL1, MAL2, and MAL3) (Basso et al., 2011). To eliminate growth on alpha-glucosides, these three MAL loci, MPH2, and MPH3 as well as the alpha glucoside hydrolase-encoding genes SUC2 and IMA1-5 were deleted, yielding S. cerevisiae IMK291 (Marques et al., 2017). Introduction of cas9 into this strain yielded IMZ616 (Marques et al., 2018).
attributes.tag: strain
attributes.value: IMZ616
attributes.tag: host
attributes.value: CEN.PK102-3A
attributes.tag: isolation_source
attributes.value: genetically engineerred
attributes.tag: collection_date
attributes.value: 2018
attributes.tag: geo_loc_name
attributes.value: Netherlands
attributes.tag: sample_type
attributes.value: pure culture
attributes.tag: genotype
attributes.value: IMZ616 MATa ura3-52 LEU2 MAL2-8C maldelta mphdelta suc2delta ima1delta ima2delta ima3delta ima4delta ima5delta pUDC156 (URA3 cas9)
attributes.tag: mating_type
attributes.value: MAT a
attributes.tag: breed
attributes.value: CEN.PK lineage
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental