title: gDNA-UV
description: Protocols: Cells were collected by treatment with accutase, a gentle enzymatic dissociation reagent for detaching adherent cells. Total DNA was isolated using phenol-chloroform extraction followed by ethanol precipitation and resuspension in nuclease-free water. Libraries were prepared using the Oxford Nanopore Native Barcoding Kit for multiplexed long-read sequencing applications.
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: female
attributes.tag: stimulus
attributes.value: UV-C(2mJ)
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: embryonic stem cell
attributes.tag: developmental stage
attributes.value: Blastocyst
attributes.tag: genotype
attributes.value: TP53:(c.451C>T),(c.542G>A),(c.743G>A),(c.799C>T)
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: cell line
attributes.value: WA09 (RRID:CVCL_9773)
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected