title: AC9-7660-T1-ITS
description: Pinus sylvestris needles collected from tree genotype 7660 at timepoint T1
attributes.tag: plant body site
attributes.value: Leaf [PO:0006001]
attributes.tag: chimera check software
attributes.value: dada2_denoise
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 25
attributes.tag: broad-scale environmental context
attributes.value: coniferous forest biome [ENVO_01000196]
attributes.tag: collection date
attributes.value: 2023-07-04
attributes.tag: target gene
attributes.value: ITS
attributes.tag: geographic location (longitude)
attributes.value: -2.89293
attributes.tag: host common name
attributes.value: Pinus sylvestris
attributes.tag: nucleic acid extraction
attributes.value: Qiagen(TM)  DNeasy Plant Pro Kitï¿½
attributes.tag: nucleic acid amplification
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf
attributes.tag: experimental factor
attributes.value: Pinus sylvestris needle phyllosphere
attributes.tag: geographic location (country and/or sea)
attributes.value: United Kingdom
attributes.tag: organism
attributes.value: phyllosphere metagenome
attributes.tag: host taxid
attributes.value: 3349
attributes.tag: environmental medium
attributes.value: plant matter [ENVO_01001121]
attributes.tag: geographic location (latitude)
attributes.value: 55.603721
attributes.tag: scientific_name
attributes.value: phyllosphere metagenome
attributes.tag: pcr primers
attributes.value: Forward: GTGARTCATCGAATCTTTG; Reverse: TCCTCCGCTTATTGATATGC
attributes.tag: local environmental context
attributes.value: experimental forest ecosystem [ENVO_03600038]
attributes.tag: project name
attributes.value: PineBiome
attributes.tag: sequencing method
attributes.value: Nextseq2000
attributes.tag: host age
attributes.value: 15 years
attributes.tag: multiplex identifiers
attributes.value: UDP0012, i5: GTGTGGCGCT, i7: GAGTCATAGG