title: E_lm_A_Irf2
description: Protocols: Macrophages lacking Irf2, Irf9, or Irf2 and Irf9, as well as scramble controls were infected with Listeria monocytogenes or uninfected under mock conditions and total RNA was harvested 2 hours post infection. Three independent experiments were performed. Gene knockouts were generated via cas9 guide transduction into cas9-expressing cells.  Total RNA was isolated using TRIzol (Fisher) and Chloroform, and the PureLink RNA Mini Kit (12183018A, Ambion). RNA was then DNase I treated (NEB), and purified using RNA clean and concentrator columns (Zymo). PolyA mRNA enrichment and library preparation was performed by Novogene Corporation Inc.
attributes.tag: ENA first public
attributes.value: 2026-04-29
attributes.tag: INSDC center name
attributes.value: UC Berkeley
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-15742:E_lm_A_Irf2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: cas9-expressing, HoxB8 conditionally-immortalized macrophages
attributes.tag: cell type
attributes.value: macrophage
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: mouse postnatal
attributes.tag: disease
attributes.value: normal
attributes.tag: genetic modification
attributes.value: Irf2 KO, generated using cas9 guide from Brie KO library (Doench et al. 2016) in Lentiguide-puro
attributes.tag: genotype
attributes.value: cas9-expressing C57BL/6
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: infect
attributes.value: Listeria Monocytogenes
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-15742:E_lm_A_Irf2
attributes.tag: scientific_name
attributes.value: Mus musculus