title: MAT18
description: Protocols: BM cells were isolated from femoral heads, otherwise discarded as surgical waste, obtained upon written informed consent from subjects undergoing hip replacement surgery due to coxarthrosis.  CD146⁺ BMSCs from each subject were plated at a density of 1.5 × 10⁵ cells per well in 6-well plates. After 3 days of incubation the total RNA was isolated using the miRNeasy Kit (QIAGEN, Hilden, Germany; cat # 217084), including an on-column DNase digestion step. RNA was subsequently precipitated with ethanol to further improve purity. Sequencing libraries were generated from 400 ng of total RNA as input with the TruSeq Stranded Total RNA kit (Illumina), according to the manufacturer's instruction.
attributes.tag: ENA first public
attributes.value: 2026-04-29
attributes.tag: INSDC center name
attributes.value: University of Salerno
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-16622:MAT18
attributes.tag: age
attributes.value: 87
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: older adults
attributes.tag: disease
attributes.value: normal
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: MAT18
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: CD146⁺ BMSCs
attributes.tag: sample name
attributes.value: E-MTAB-16622:MAT18
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male