title: WT
description: Protocols: Thymic cells were isolated from three female C57BL/6 mice. The thymuses were dissected and processed through a 40 μm filter to obtain single-cell suspensions. Cells were stained and sorted by flow cytometry, excluding CD19⁺ (B cells), Ter119⁺ (erythroid cells), Ly6G/C⁺ (granulocytes), CD11b⁺ (myeloid cells), CD4⁺ and CD8⁺ (mature T cells), while selecting for Thy1.2⁺ cells. This strategy ensured enrichment for early thymic progenitors in the double negative stage. Nucleic acid extraction was performed as part of the library construction. Libraries were generated using the Chromium Next GEM Single Cell 3' Kit v3.1 (10X Genomics), according to the manufacturer's instructions
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: female
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: cell type
attributes.value: double negative thymocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: organism part
attributes.value: thymus
attributes.tag: developmental stage
attributes.value: juvenile days 45-89
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: age
attributes.value: 72
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected