ENA first public
2026-04-30
INSDC center name
The James Hutton Institute
INSDC status
public
Submitter Id
pbITs00151
broad-scale environmental context
coniferous forest biome [ENVO_01000196]
chimera check software
dada2_denoise
collection date
2023-07-05
environmental medium
plant matter [ENVO_01001121]
experimental factor
Pinus sylvestris needle phyllosphere
geographic location (country and/or sea)
United Kingdom
geographic location (latitude)
55.603721
geographic location (longitude)
-2.89293
host age
15 years
host common name
Pinus sylvestris
host taxid
3349
local environmental context
experimental forest ecosystem [ENVO_03600038]
multiplex identifiers
UDP0200, i5: AATGTATTGC, i7: GAGGTTAGAC
nucleic acid amplification
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf
nucleic acid extraction
Qiagen(TM) DNeasy Plant Pro Kit�
pcr primers
Forward: GTGARTCATCGAATCTTTG; Reverse: TCCTCCGCTTATTGATATGC
plant body site
Leaf [PO:0006001]
project name
PineBiome
sample name
pbITs00151
sample volume or weight for DNA extraction
25
scientific_name
phyllosphere metagenome
sequencing method
Nextseq2000
target gene
ITS