strain
BDS-LM099; HPB_1088; HPB 1088; HPB-1088; HPB1088
isolate
BDS-LM099_passage0
collected_by
Health Canada (HC)
collection_date
1994
geo_loc_name
Canada
isolation_source
clinical
lat_lon
not collected
host
Homo sapiens (human)
host_disease
missing
host_tissue_sampled
not specified
passage_history
SOP for the creation and storage of “Passage 0” freezer stock of benchmark strains was followed (v1.1, https://www.protocols.io/view/handling-of-the-listeria-monocytogenes-benchmarks-d5nw85fe): Culture collection freezer stocks were streaked onto BHIA and grown 24±2 hours at 36±3ºC. An isolated colony was then streaked onto RLM (or BHIA) agar and incubated for 24±2 hours at 36±3ºC. A single colony was used to inoculate 5 mL BHI and grown 24±2 hours at 36±3ºC. This established a uniform culture protocol to prepare a 15% glycerol “Passage 0” benchmark panel stock stored at ≤70ºC, and also use for DNA extraction and whole genome sequencing.
serotype
4d
BioSampleModel
Pathogen.cl