strain
BDS-LM122; HPB_477; HPB 477; HPB-477; HPB477
isolate
BDS-LM122_passage0
collected_by
Health Canada (HC)
collection_date
missing
geo_loc_name
missing
isolation_source
Unknown
lat_lon
not collected
passage_history
SOP for the creation and storage of “Passage 0” freezer stock of benchmark strains was followed (v1.1, https://www.protocols.io/view/handling-of-the-listeria-monocytogenes-benchmarks-d5nw85fe): Culture collection freezer stocks were streaked onto BHIA and grown 24±2 hours at 36±3ºC. An isolated colony was then streaked onto RLM (or BHIA) agar and incubated for 24±2 hours at 36±3ºC. A single colony was used to inoculate 5 mL BHI and grown 24±2 hours at 36±3ºC. This established a uniform culture protocol to prepare a 15% glycerol “Passage 0” benchmark panel stock stored at ≤70ºC, and also use for DNA extraction and whole genome sequencing.
BioSampleModel
Pathogen.env