Generic sample from Loxodonta africana
Loxodonta africana
Loxodonta africana placental tissue was kindly provided by Dr. Kurt Benirschke of the San Diego Zoo and preserved in RNAlater (Ambion, Foster City, CA).  Placental villous trees were separated from the extraembryonic membranes, and total RNA was isolated from the pooled placental compartments using TRIzol Reagent (Invitrogen, Carlsbad, CA).  The Qiagen RNeasy kit was used in conjunction with the Qiagen Rnase-Free Dnase Set for cleanup (Qiagen, Valencia, CA, USA) according to the manufacturer 2019s recommendations.  The concentration and purity of the total RNA isolated from the placental villous trees was tested by using the Nanodrop ND-1000 Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE).  Total RNA (15*g) was submitted to Agencourt Bioscience Corporation (Beverly, MA, USA) per the company’s instructions.