KO377p3b
Mus musculus
Protocols: After subcutaneous growth, the tumors were collected and frozen in liquid nitrogen. RNA extraction was performed from 30 mg of frozen tumor fragments using the RNAeasy kit (Qiagen) according to the manufacturer's instructions. During cell lysis step, tumor fragments were crushed with TissueRuptor (Qiagen) in RLT buffer supplemented with 40mM dithiothreitol (DTT). RNA concentration and quality was assessed with Bioanalyzer instrument (Agilent). Sequencing libraries were generated using NEBNext Ultra RNA library Prep Kit for Illumina following manufacturer's recommendations.
ENA-FIRST-PUBLIC
2020-07-01T04:04:32Z
ENA-LAST-UPDATE
2020-06-17T09:41:45Z
External Id
SAMEA6968137
INSDC center name
CNRS, UMR 9018, Gustave Roussy and Universite Paris-Saclay
INSDC first public
2020-07-01T04:04:32Z
INSDC last update
2020-06-17T09:41:45Z
INSDC status
public
Submitter Id
E-MTAB-9220:KO377p3b
age
6 to 8
broker name
ArrayExpress
cell line
MB49 mouse bladder carcinoma cell line
common name
house mouse
genotype
gal-9 knockout
growth condition
serial transplantations into syngenic mice
organism part
subcutaneous neoplasm
sample name
E-MTAB-9220:KO377p3b
sampling time point
3rd growth cycle
scientific_name
Mus musculus
sex
female
strain
C57BL/6N