NA
Homo sapiens
NA
isolate
Not applicable
age
Not applicable
biomaterial_provider
Center for Infectious Medicine, Karolinska Institutet, ANA 8, Stockholm, Sweden
sex
Not applicable
tissue
Not applicable
cell_line
N/TERT-1, primary fibroblsts, primary monocytes
cell_subtype
NA
cell_type
Keratinocytes, fibroblasts, monocytes
culture_collection
NA
dev_stage
NA
disease
NA
disease_stage
NA
ethnicity
NA
health_state
NA
karyotype
NA
phenotype
NA
population
NA
race
NA
sample_type
3D organotypic skin tissue model
treatment
The organotypic tissue was generated following the protocol of established lung tissue model with minor modifications and the addition of 1*10^6 primary monocytes from donors.
infectionandtime
Models were infected with a clincial GAS isolate (GAS 2006; emm1 type) collected from an NSTI patient enrolled in the INFECT study. Skin models were infected with an infectious dose of 1x106 bacteria on top of the stratified keratinocytes for 8 hours
SamplesSequence
6
BioSampleModel
Human