Generic sample from Dermacentor variabilis
Dermacentor variabilis
Synganglia from female American dog ticks, Dermacentor variabilis, were dissected from unfed, partially-fed virgin, partially-fed virgins forcibly detached and incubated for 4 d, partially-fed mated, replete and ovipositing ticks.  Pooled mRNA was reverse transcribed with a modified oligo-dT (see Beldade et al. 2006) and in the presence of SMART IV oligo.  cDNA was PCR amplified and used for GS FLX library preparation.