NA
Homo sapiens
NA
isolate
Not applicable
age
Not applicable
biomaterial_provider
Center for Infectious Medicine, Karolinska Institutet, ANA 8, Stockholm, Sweden
sex
Not applicable
tissue
Not applicable
cell_line
N/TERT-1, primary fibroblsts, primary monocytes
cell_subtype
NA
cell_type
Keratinocytes, fibroblasts, monocytes
culture_collection
NA
dev_stage
NA
disease
NA
disease_stage
NA
ethnicity
NA
health_state
NA
karyotype
NA
phenotype
NA
population
NA
race
NA
sample_type
3D organotypic skin tissue model
treatment
The organotypic tissue was generated following the protocol of established lung tissue model with minor modifications and the addition of 1*10^6 primary monocytes from donors.
infectionandtime
Models were infected with a clincial GAS isolate (GAS 2006; emm1 type) collected from an NSTI patient enrolled in the INFECT study. Skin models were infected with an infectious dose of 1x106 bacteria on top of the stratified keratinocytes for 24 hours
SamplesSequence
7
BioSampleModel
Human