wt.60min.cadmium
Schizosaccharomyces pombe
Protocols: Cells were treated for 15 min or 60 min (as indicated) with 0.5 mM CdSO4 (cadmium). Cells were collected by filtration. Filters were immediately frozen in liquid nitrogen. Total RNA was extracted using a hot phenol extraction method as described in Lyne et al. (2003) BMC Genomics 4:27. Clean up and DNase treatment were performed on with Purelink RNA microcolumns (Life Technologies) following the manufacturer's instructions. Samples were ribo-depleted using Ribo-Zero Gold rRNA Removal Kit Yeast (Illumina) with a 4 ug input. 30 ng of ribo-depleted RNA was used as starting material for the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific).
ENA-FIRST-PUBLIC
2020-06-01T04:03:51Z
ENA-LAST-UPDATE
2019-12-11T14:20:12Z
External Id
SAMEA6379797
INSDC center name
Department of Biochemistry University of Cambridge
INSDC first public
2020-06-01T04:03:51Z
INSDC last update
2019-12-11T14:20:12Z
INSDC status
public
Submitter Id
E-MTAB-8583:wt.60min.cadmium
broker name
ArrayExpress
common name
fission yeast
genotype
wild type genotype
replicate
A
sample name
E-MTAB-8583:wt.60min.cadmium
scientific_name
Schizosaccharomyces pombe
stimulus
cadmium sulfate