SL2302
Chlamydomonas reinhardtii
Protocols: C. reinhardii strains were obtained from the Chlamydomonas Resource Center (University of Minnesota) and maintained by stricking cells into new fresh solid media every two months. Media 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIS)-acetate-phosphate (TAP) media (Harris 2009) in the presence of 15% agar. C. reinhardtii was grown in liquid TAP for a few days with constant shaking at 25 ᴼC under continuous illumination. Cells were harvested when cultures reached saturation Total RNA was extracted with Trizol reagent by following a protocol previously described (Molnar et al., 2007, Nature). Cloning of small RNAs was performed directly from total RNAs by using the Illumina TruSeq v2 RNA Sample Preparation Kit
ENA-FIRST-PUBLIC
2020-06-01T04:03:50Z
ENA-LAST-UPDATE
2019-11-15T17:03:29Z
External Id
SAMEA6229569
INSDC center name
Department of Plant Sciences University of Cambridge
INSDC first public
2020-06-01T04:03:50Z
INSDC last update
2019-11-15T17:03:29Z
INSDC status
public
Submitter Id
E-MTAB-8526:SL2302
broker name
ArrayExpress
developmental stage
vegetative
ecotype
CC1883
genotype
wild type genotype
organism part
whole organism
sample name
E-MTAB-8526:SL2302
scientific_name
Chlamydomonas reinhardtii