PAO1_WT_S2
Pseudomonas aeruginosa PAO1
Protocols: Bacteria were grown in BHI medium at 37°C under shaking conditions (200 rpm). Cells were harvested by centrifugation after reaching OD600 2.7 . Pellets were frozen at -80°C. Bacterial pellets were resupended in 200 µl of 1x Tris-EDTA buffer (pH 8.0) containing 100 µg/ml lysozyme and 20 µg/ml Proteinase K. RNA was extracted using Trizol reagent according the provided protocol. RNA was purified from aqueous phase using Zymo Research RNA Clean & concentrator kit. rRNA was depleted using Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) and library was prepared following protocol provided with Illumina TruSeq Stranded mRNA Library Prep Kit.
ENA first public
2018-02-05
ENA last update
2017-11-24
External Id
SAMEA104402394
INSDC center alias
Department of Clinical Microbiology, Rigshospitalet
INSDC center name
Department of Clinical Microbiology, Rigshospitalet
INSDC first public
2018-02-05T17:02:59Z
INSDC last update
2017-11-24T15:09:58Z
INSDC status
public
Submitter Id
E-MTAB-6247:PAO1_WT_S2
broker name
ArrayExpress
genotype
wild type genotype
growth condition
Brain Heart Infusion (BHI) medium. 37C. Shaking 200 rpm.
sample name
E-MTAB-6247:PAO1_WT_S2