HCL_448_A_TSCM_CM
Homo sapiens
Protocols: T cells were FACS-sorted from total PBMCs of six healthy donors in duplicate (150,000 cells/subset for each replicate) in 1.2 mL RLT buffer (Qiagen; final dilution < 20%) for cell lysis and stored at -80°C Total RNA was extracted using RNeasy Mini Kit (Qiagen) 5′ RACE TCRβ sequencing libraries with Unique Molecular Identifiers (UMIs) were prepared using a Human TCR Profiling Kit (MiLaboratory) according to the recommended procedure.
ENA-FIRST-PUBLIC
2020-08-02T04:06:10Z
ENA-LAST-UPDATE
2020-03-11T16:33:48Z
External Id
SAMEA6619930
INSDC center name
Central European Institut of Technology, Masaryk University
INSDC first public
2020-08-02T04:06:10Z
INSDC last update
2020-03-11T16:33:48Z
INSDC status
public
Submitter Id
E-MTAB-8892:HCL_448_A_TSCM_CM
broker name
ArrayExpress
cell type
TSCM
common name
human
disease
normal
individual
448
organism part
blood
replicate
1
sample name
E-MTAB-8892:HCL_448_A_TSCM_CM
scientific_name
Homo sapiens