Mycobacterium tuberculosis variant bovis BCG, Pasteur 1721, SapM transposon mutant
Mycobacterium tuberculosis variant bovis BCG
The M. bovis BCG Pasteur strain 1721, used to create the sapMTn BCG mutant strain (Festjens et al, EMBO Mol Med 2011; Vandewalle et al, Nat Commun 2015), differs from the 1173P2 Pasteur vaccine strain by a K43R point mutation in the rpsL gene, conferring streptomycine resistance (Master et al, Cell Host Microbe 2008), which is useful to avoid contaminations during the lengthy and involved procedures in ordered transposon mutant library production. To investigate whether there are any additional variants between the WT BCG Pasteur and the sapMTn BCG mutant, we performed a whole-genome resequencing analysis on both strains (paired end 2 x 150 bp), mapping to the M. bovis BCG Pasteur 1173P reference genome, resulting in ~80x average coverage of mapped reads
strain
Pasteur 1721
isolation_source
Lab strain
collection_date
2012-04-15
geo_loc_name
Belgium:Ghent
sample_type
bacterial culture
BioSampleModel
Microbe, viral or environmental