Naumovozyma castellii
N. castellii RNAi mutant 6 isolated in genetic selection for RNAi mutants.
strain
CBS4310
host
Not applicable
isolation_source
Not applicable
collection_date
2012-10-15
geo_loc_name
Not applicable
sample_type
YPD culture inoculated from a single colony
collected_by
Matthew Getz
genotype
MAT a ho(delta) ura3::EGFP(S65T)::kanMX6 ade2(delta) his3(delta) leu2(delta) lys2(delta) trp1(delta)::HYG ncas_Chr_9::pIp-strongSC_GFP ncas_Chr_3::pRS403-ScerURA3hp-HIS3-PEST ncas_Chr_4::pRS405-ScerHIS3hp ncas_Chr_5::pRS402-NcasDCR1 ncas_Chr_3::pRS404-NcasAGO1
mating_type
MAT a
passage_history
Mutant selected on SGal + Lys solid media, streaked once for single colonies on SGal + Lys solid media from which a YPD glycerol stock was prepared. YPD glycerol stock was streaked once on SGal + Lys and single colony was selected to inoculate YPD culture.
temp
Grown at 25°C during mutant selection. Otherwise grown at 30°C.
mutant_order_of_isolation_and_screening
6
media_selected_on
SGal + Lys
BioSampleModel
Microbe, viral or environmental