Soft-chhurpi
food fermentation metagenome
Naturally fermented milk  (NFM) products
host
Bos taurus
isolation_source
Fermented milk products
collection_date
2016-03-25
geo_loc_name
India: Sikkim
lat_lon
27.3032 N 88.2072 E
collection_time
10:00:15
replicated_pcr_that_are_pooled
2
pcr_clean_up_methods
gel, column
name_of_institute_creating_the_library
Xcelris Genomics, Ahmedabad, India
sequencing_method_used
Illumina (MiSeq)
targeted_gene_or_locus_name_for_marker_gene_studies
16S rRNA gene
url_of_instatute_sequencing_was_done
https://www.xcelrislabs.com/
sequencing_direction
both
name_of_subfragment_of_a_gene_or_locus
V4-V5
list_of_forward_pcr_primers
AYTGGGYDTAAAGNG
list_of_reverse_pcr_primers
CCGTCAATTCMTTTRAGT
thermocycler_make_and_model
BIORAD, C1000
tag_polymerase_used_for_amplification
Phusion High-Fidelity DNA Polymerase
number_of_thermocycles
28
cycle_annealing_time_in_seconds
30
cycle_annealing_temperature_in_celsius
55
cycle_annealing_method
static
cycle_denaturation_temperature_in_celsius
98
cycle_extension_temperature_in_celsius
72
initial_denaturation_temperature_in_celsius
98
final_extension_temperature_in_celsius
72
forward_primer_final_concentration_in_micromolar
0.1
reverse_primer_final_concentration_in_micromolar
0.1
datp_final_concentration_in_micromolar
50
dctp_final_concentration_in_micromolar
50
dgtp_final_concentration_in_micromolar
50
dttp_final_concentration_in_micromolar
50
mgcl2_final_concentration_expressed_in_millimolar
2.5
BioSampleModel
Metagenome or environmental