Generic sample from Homo sapiens
Homo sapiens
RNA sequencing of Stratagene's human universal reference RNA (UHR).  In two experiments we compared the effect of using the phi X control lane for base-calling.  Both these two experiments used the exact same image files to generate base-calls using phi X calibration or auto calibrartion.   In for separate experiments, four different library preparations were made and the results sequenced.  Note that since the input is commercial RNA, there is no RNA extraction process involved.