p300_gastrula
Nematostella vectensis
source_name
gastrulae (24h)
development stage
gastrulae (24h)
chip antibody
Nematostella p300/CBP
chip antibody source
We used a 146 aa peptide of the C-terminus of Nematostella p300 protein, including a N-terminal 10x His tag for immunization of two rabbits each. The p300 peptide sequence was HPNSQAQQPPGQHYQAKQMQPRLMSPQAPPPMSPQQGGLPSQSPRPVMSPQPHANTLSPRQQTMSPAHPQQSLTSPRPAQSPHQSHMDPSNLGDKPNLFTQPLSARMTLTPLAAPSESDMSLGQEDSETVPPLTPQDQLTKFVENL. P300 antisera and purified p300 antibodies were generated by Primm (www.primmbiotech.com).
fragmentation
sonication
library method
ChIP-Seq
read length
50