PAL_3_2_2008_01_12
marine metagenome
Palmer Station LTER metagenome;archaea
collection_date
2008-01-12
biome
seawater
feature
marine_habitat
material
water
geo_loc_name
Polar Antarctic Province
lat_lon
-130.83
rel_to_oxygen
Oxic
samp_collect_device
Niskin
samp_mat_process
NONE
depth
12.765
chlorophyll
0.259 microgramPerLiter
density
27.194
diss_org_carb
46.2 microMoleCarbonPerLiter
diss_oxygen
353 microMolePerLiter
elev
0
nitrate
21.3 microMolePerLiter
nitrite
0.4 microMolePerLiter
part_org_carb
80.7 microMolePerLiter
part_org_nitro
16.9 microMolePerLiter
phosphate
1.7 microMolePerLiter
salinity
33.9072 PSU
samp_size
2 liter
silicate
32 microMolePerLiter
temp
0.5633 celsius
tot_depth_water_col
3090 meter
tot_nitro
32.3 microMolePerLiter
experimental_factor
seasonal/depth
investigation_type
MIMARKS_Survey
lter_station_name
600.2
ogl_reference_name
PAL_3_2_2008_01_12
sample_type
water
environment
ENVO:seawater;ENVO:marine_habitat;ENVO:water
env_package
water
depth_category
top 50 m
nitrite_plus_nitrate
21.7 microMolePerLiter
fluorescence
0.4863 milligramPerMeterCubed
leucine
7.8 picoMolePerLiter
carbon_14
6.08 milliMolesCarbonPerMeterSquaredPerDay
bottle
19
cast
21
lib_reads_seqd
11
multiplex_ident_1
ACTGC
nucl_acid_amp
DOI: 10.1126/science.114668
domain
Archaea
pcr_primers
958FWD:AATTGGANTCAACGCCGG;1048REV-major:CGRCGGCCATGCACCWC;1048REV-minor:CGRCRGCCATGYACCWC
target_gene
16S rRNA
target_subfragment
V6
nucl_acid_ext
bead beating alkaline lysis
pcr_cond
initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;28 cycles
sequencing_meth
GS FLX LR70 454 pyrosequencing
url
http://amarallab.mbl.edu/mirada/mirada.html; http://vamps.mbl.edu
bacteria_count
0.173 X106perML
conductivity
2.87 seimenPerMeter
atm_pressure
12.891 db
decimal_time
5.2
shelf_position
OFF
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.water