Generic sample from Caenorhabditis elegans
Caenorhabditis elegans
 Embryos were collected by bleaching from mixed stage hermaphrodite populations. The embryos surviving bleach treatment were incubated without food, causing them to arrest after hatching as L1 stage larvae. Starved L1 larvae were plated in the presence of food and raised at 25 degrees Celsius. Staging was determined by examining a subset of animals under Nomarski optics at intervals for vulval and germline development. Animals were collected at the mid-L2, mid-L3, mid-L4, and young adult (pre-gravid) stages and washed in S basal buffer. Exact times for growth after plating of starved L1s (all at 25 degrees C) were as follows: (1) L1 worms were grown for 4 hours. Run 30K6TAAXX was labeled L1_ce0077_rw007 and had unexpectedly short inserts with sequences reading through to the linker. They took the same size-fractionated DNA and ran it on another gel. Subsequent runs were then labeled as L1_ce0132_rw007. Reads are in read pairs. The _1 files are the forward reads and the _2 files are the reverse reads. The forward and reverse reads have the same name except there is a trailing /1 on the forward reads and /2 on the reverse reads. Quality values are Solexa/Illumina quality values. 