Sample3_863B-P2 _Leaf transcriptome
Cenchrus americanus
Pot-grown plants were subjected to drought stress using a standard dry-down protocol (Kholova et al., 2010) at boot leaf stage under controlled conditions in green house. The drought stressed boot leaf tissue of 863B-P2 were sampled from 3 replicates at the four stages viz., (i) 4 days after initiation of the drought stress treatment, (ii) 70% normalized transpiration ratio (NTR), (iii) 40% NTR and at (iv) 20% NTR and were pooled for the respective stage. The total RNA extracted (Schmitt et al., 1990) from each stage was normalized and pooled to get one sample. cDNA was synthesized from this sample using Super SMART PCR cDNA synthesis protocol and was used for sequencing using GS FLX system.
ENA-FIRST-PUBLIC
2014-02-15T17:01:59Z
ENA-LAST-UPDATE
2018-03-09T10:00:56Z
External Id
SAMEA2020603
INSDC center name
ICRISAT
INSDC first public
2014-02-15T17:01:59Z
INSDC last update
2018-03-09T10:00:56Z
INSDC status
public
Submitter Id
Sample3_863B-P2 _Leaf
sample name
Sample3_863B-P2 _Leaf
scientific_name
Cenchrus americanus