Rhesus macaque lymphoblast 2
Macaca mulatta
Protocols: Lymphoblast cell lines were obtained for seven primate species. All cell lines were transformed by the Epstein Barr virus except for the macaque cell lines that were transformed by a herpesvirus. Cells were grown in suspension at a confluency of 200 000-1 x106 cells/ml in RPMI1640 media supplemented with 10% Fetal Bovine Serum and 2 mM L-glutamine. C57BL/6J mice were housed in the Biological Resources Unit under UK Home Office licensing. Tissue was obtained from at least two independent males and cross-linked as described in (Schmidt, Methods,2009). Male and female human tissue samples were obtained from biopsied tissue collected at Addenbrooke's Hospital, Cambridge, and were provided by the Biobank under human tissue license 08/H0308/117. Liver tissue was also obtained from the Liver Tissue Distribution Program (NIDDK contract number N01-DK-9-2310) at the University of Pittsburgh. 1x10^8 cells were cross-linked with 1% formaldehyde as previously described (Schmidt, Methods,2009). ChIP-seq assays were performed as previously described (Schmidt, 2009). Protein-bound DNA was immunoprecipitated with antibodies against CTCF (Millipore, 07-729), or YY1 (Santa Cruz, sc-281).
ENA-FIRST-PUBLIC
2013-04-22T17:03:04Z
ENA-LAST-UPDATE
2018-03-08T16:17:59Z
External Id
SAMEA1877863
INSDC center name
EFPL
INSDC first public
2013-04-22T17:03:04Z
INSDC last update
2018-03-08T16:17:59Z
INSDC status
public
Submitter Id
E-MTAB-1511:Rhesus macaque lymphoblast 2
broker name
ArrayExpress
cell line
150-99
cell type
lymphoblastoid
common name
Rhesus monkey
sample name
E-MTAB-1511:Rhesus macaque lymphoblast 2
scientific_name
Macaca mulatta