Generic sample from Pinus monticola
Pinus monticola
Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
barcode AACT
Pinus monticola MONT06E1
barcode ATGT
Pinus monticola MONT07E2
barcode CACT
Pinus monticola MONT08E1
barcode GTAT
Pinus monticola MONT12E1
barcode GGGT
Pinus monticola MONT14E1