Genotyping-by-sequencing (GBS) of Coffea arabica with ADN-CODE "14CO637" and customer code "T.04591".
Coffea arabica
Genomic DNA was digested using the restriction enzyme PstI. 794 Coffea arabica, 54 Coffea canephora, and 10 Coffea eugenoides genotypes were barcoded and pooled in groups of 96 and sequenced on an Illumina HiSeq 2000 using a 91bp single-end sequencing strategy. Sequence data was un-multiplexed and the Illumina adapter sequences were removed using Trimmomatic-0.36. The resulting sample specific and trimmed fastq data is submitted here and was used in downstream GBS analyses.
cultivar
T.04591_14CO637
dev_stage
not collected
geo_loc_name
Ethiopia
tissue
Leaf
biomaterial_provider
CATIE
lat_lon
7.14 N 36.24 E
BioSampleModel
Plant