2A_2i_1
Mus musculus
Protocols: ES cells were cultured on 0.1% gelatin-coated plates in 2i/LIF conditions. For neural differentiation, ES cells were plated onto laminin-coated plates in N2B27 media at a density of 100,000 per 6-well. Medium was replaced every day and the cells were not passaged during the timecourse. RNA was extracted with TRI Reagent (Sigma). Libraries were prepared with the NEXTflex Rapid Directional Illumina RNA-seq Library Prep Kit (Bioo Scientific)
ENA first public
2016-08-09
ENA last update
2016-03-17
External Id
SAMEA3903097
INSDC center alias
University of Cambridge
INSDC center name
University of Cambridge
INSDC first public
2016-08-09T17:01:30Z
INSDC last update
2016-03-17T08:09:43Z
INSDC status
public
Submitter Id
E-MTAB-4566:2A_2i_1
broker name
ArrayExpress
cell type
embryonic stem cell
common name
house mouse
genotype
Sall4 knock out
sample name
E-MTAB-4566:2A_2i_1