HH038_NASH
Homo sapiens
source_name
Hepatocytes from NASH patient
biomaterial_provider
De-identified normal human liver tissue and/or cells were obtained through the Liver Tissue Cell Distribution System (Pittsburgh, PA) after obtaining written informed consent by a protocol approved by the Human Research Review Committee of the University of Pittsburgh, which was funded by NIH Contract # HSN276201200017C. Adult human liver tissue and/or cells were also obtained from Ira J Fox Laboratory at Children's Hospital of UPMC, after obtaining written informed consent by a protocol approved by the Human Research Review Committee and the Institutional Review Board (IRB#: PRO12090466) of the University of Pittsburgh. Hepatocytes were isolated using a three-step collagenase digestion technique. Cell viability was assessed after isolation as previously described using trypan blue exclusion and only cell preparations with viability >80% were used for the analysis.
individual
NASH patient
cell type
isolated primary hepatocytes