miR-34a-/- HCT-116
Homo sapiens
Cells were transfected with two pSpCas9(BB)-2A-GFP plasmids designed to target each side of the miR-34a hairpin locus, then selected by FACS single-cell sorting and homozygous mutant clones were validated by PCR and Sanger sequencing of the miR-34a locus.
isolate
HCT-116 cell from ATCC (cat. # CCL-247), individual miR-34a-/- clone obtained after CRISPR/Cas9 mutagenesis and selection
age
Not applicable
biomaterial_provider
Seitz lab, IGH (CNRS and Montpellier university), Montpellier, France
sex
male
tissue
Cell line
cell_line
HCT-116
culture_collection
ATCC:CCL-247
sample_type
Cell culture
BioSampleModel
Human