the midgut of Nilaparvata lugens
Nilaparvata lugens
Three to four-instar nymphs of N. lugens were collected . Before dissection, the insects were chilled on ice and placed in a petri dish that had been brushed with insect physiological buffer (0.65% NaCl water solution). The dissected midguts were immediately placed in TRIzol reagent (Invitrogen). Three hundred midguts were collected for total RNA extraction. The N. lugens population was reared on TN1 rice plants, which is susceptible to N. lugens, in the greenhouse at the Genetics Institute, Wuhan University.