FRC_TCDBM+T_29
Mus musculus
Protocols: LN were isolated from recipient mice and digested with Liberase/Dnase at 37°C. Single cell suspension was stained for CD45, gp38 and CD31 to discriminate different lymph node stroma subsets. CD45-CD31-gp38+ FRC were flow sorted and collected in RLT lysis buffer. RNA was extracted using the RNeasy Micro Kit (QIAGEN, USA) following the manufacturers protocol. RNA yield, quality and integrity were evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA samples were amplified using the SMART Seq v4 Ultra Low Input RNA Kit. Paired-end sequencing libraries were prepared from the amplified cDNA according to the Nextera XT DNA library prep protocol.
ENA-FIRST-PUBLIC
2020-01-14T04:04:50Z
ENA-LAST-UPDATE
2019-08-16T13:45:53Z
External Id
SAMEA5870251
INSDC center name
UCL Cancer Institute and Institute of Immunity and Transplantation
INSDC first public
2020-01-14T04:04:50Z
INSDC last update
2019-08-16T13:45:53Z
INSDC status
public
Submitter Id
E-MTAB-8255:FRC_TCDBM+T_29
age
10
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
disease
acute graft vs. host disease
genotype
wild type genotype
individual
29
organism part
lymph node
sample name
E-MTAB-8255:FRC_TCDBM+T_29
scientific_name
Mus musculus
sex
male
stimulus
T-cell depleted bone marrow + T cells transplant
strain
C57BL/6