fil1.15min.heatshock
Schizosaccharomyces pombe
Protocols: Cells were grown for 15 min or 60 min at 39C. Cells were collected by filtration. Filters were immediately frozen in liquid nitrogen. Total RNA was extracted using a hot phenol extraction method as described in Lyne et al. (2003) BMC Genomics 4:27. Clean up and DNase treatment were performed on with Purelink RNA microcolumns (Life Technologies) following the manufacturer's instructions. Samples were ribo-depleted using Ribo-Zero Gold rRNA Removal Kit Yeast (Illumina) with a 4 ug input. 30 ng of ribo-depleted RNA was used as starting material for the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific).
ENA-FIRST-PUBLIC
2020-06-01T04:03:51Z
ENA-LAST-UPDATE
2019-12-11T14:20:12Z
External Id
SAMEA6379785
INSDC center name
Department of Biochemistry University of Cambridge
INSDC first public
2020-06-01T04:03:51Z
INSDC last update
2019-12-11T14:20:12Z
INSDC status
public
Submitter Id
E-MTAB-8583:fil1.15min.heatshock
broker name
ArrayExpress
common name
fission yeast
genotype
fil1 deletion
replicate
B
sample name
E-MTAB-8583:fil1.15min.heatshock
scientific_name
Schizosaccharomyces pombe
stimulus
heat shock