Treated biocrust from Arabah site — hyperarid
soil metagenome
This is a hyperarid biocrust which was obtained from the Arabah region (29°58′ N 35°3′ E ∼140 m a s l) - a dry valley part of the Jordan Rift Valley with mean annual precipitation of ∼20 mm and potential evapotranspiration of ∼2500 mm yr−1 giving it an aridity index of AIU = 0.009. The soil at this site is alkaline (pH ∼8.5) and calcareous.
ENA-FIRST-PUBLIC
2013-04-20
ENA-LAST-UPDATE
2012-12-21
External Id
SAMEA1877561
INSDC center alias
MPI-M
INSDC center name
Max Planck Institute for Terrestrial Microbiology
INSDC first public
2013-04-20T17:03:01Z
INSDC last update
2012-12-21T16:13:16Z
INSDC status
public
Submitter Id
HAD3W_C_1_H
chimera check
UCHIME
collection date
2010-12-10
current land use
Native
depth
0.003
environment (biome)
Hyperarid desert
environment (feature)
Biological soil crust
environment (material)
Soil
environmental package
soil
geographic location (country and/or sea,region)
Israel
geographic location (elevation)
140
geographic location (latitude)
29.58
geographic location (longitude)
35.03
horizon
biocrust
investigation type
mimarks-survey
mean annual and seasonal precipitation
20
miscellaneous parameter
Hydrated with 16-O H2O; incubated under dark—anoxic conditions for 3 weeks; heavy isotopic fraction
multiplex identifiers
AGTCAGTC
nucleic acid extraction
http://www.nature.com/protocolexchange/protocols/2484
pH
8.5
pcr primers
343Fmod:TACGGGWGGCWGCA;784Rmod:GGGTMTCTAATCCBKTT
project name
H2O-SIP
relevant standard operating procedures
http://www.mothur.org/wiki/Schloss_SOP
sample name
HAD3W_C_1_H
sample weight for DNA extraction
0.5
sequencing method
pyrosequncing 454 GS FLX Titanium system
storage conditions (fresh/frozen/other)
Frozen
target gene
Bacterial 16S rRNA
water content of soil
0.4
water content of soil method
Gravimetric