wt HAP1
Homo sapiens
Cells were transfected with VP12 plasmid (expressing Cas9 and no guide RNA), then selected by FACS single-cell sorting and homozygous wild-type clones were validated by PCR and Sanger sequencing of the miR-34a locus.
isolate
HAP1 cells from Horizon Discovery (cat. # C631), individual wt clone obtained after mock CRISPR/Cas9 mutagenesis and selection
age
Not applicable
biomaterial_provider
Seitz lab, IGH (CNRS and Montpellier university), Montpellier, France
sex
male
tissue
Cell line
cell_line
HAP1
culture_collection
not applicable
sample_type
Cell culture
BioSampleModel
Human