Generic sample from Homo sapiens
Homo sapiens
HT-29 cells were cultured in RPMI1640 or McCoy's 5A medium supplemented with 10% fetal bovine serum. Cells were incubated with 5Aza (Sigma) (500nM) for 72 h; the culture medium was replaced with fresh medium plus drug 24 h after initiation of incubation. Total RNA for 5'SAGE analysis and for RT-PCR was extracted using RNA-Bee. The quality of total RNA from each cell line was analyzed using an Agilent 2100 Bioanalyzer.
cell-line
HT-29