Homo sapiens
Sequences from Guilinger, et al. Nature Methods, 2014.  The specificity profiles of TALENs targeting CCR5A target sites using an in vitro selection method.  Briefly, preselection libraries of > 1000000000000 DNA sequences, each theoretically containing at least 10 copies of all possible DNA sequences with six or fewer mutations relative to the on-target sequence (TTCATTACACCTGCAGCT-random spacer-AGTATCAATTCTGGAAGA), were digested with 4 nM to 32 nM of an in vitro-translated TALEN. Cleaved library members harbored a free 5' monophosphate that enabled them to be captured by adaptor ligation.  We isolated DNA fragments of length corresponding to 1.5 target sites (an intact target site and a repeated half-site up to the point of TALEN-induced DNA cleavage) by gel purification. High-throughput sequencing of TALEN-treated or control samples that survived this selection revealed the abundance of all TALEN-cleaved sequences as well as the abundance of the corresponding sequences before selection.  In the control sample, all members of the preselection library were cleaved by a restriction endonuclease at a constant sequence to enable their capture by adaptor ligation and isolation by gel purification. 
 Selections were indexed with the first 5 bp of sequence.  Thus, the first 5bp of each sequences identifies which selection condition the sequence originated from.  The indexing is as follows (with selection names corresponding to conditions given in Supplementary Table 1): for sequences in CCR5TALset1_S9 files: "ACTGT" = CCR5A 32 nM canonical, "CTGAA" = CCR5A 16 nM canonical, "TGACT" = CCR5A 8 nM canonical, "TGCAA" = CCR5A 4 nM canonical, "GCATT" = CCR5A 32 nM Q7, "CATGA" = CCR5A 16 nM Q7, "ATGCT" = CCR5A 8 nM Q7, "CTAGT" = CCR5A 4 nM Q7, "GCTAA" = CCR5A Q3, "CAGTT" = CCR5A 28-aa, "GTCAT" = CCR5A canonical ELD/KKR, "ACGTA" = CCR5A Q3 ELD/KKR.
 For sequences in CCR5TALset2_S10 files: "ACTGT" = CCR5A N1, "CTGAA" = CCR5A N2, "TGACT" = CCR5A N3, "CATGA" = CCR5A N2 ELD/KKR, "ACGTA" = CCR5A Q7 ELD/KKR
 Pre-selection library sequences are in CCR5ALib_S11 files
 
isolate
synthetic genomic site
age
NA
biomaterial_provider
IDT
sex
not determined
tissue
NA
BioSampleModel
Human