Generic sample from Homo sapiens
Homo sapiens
The THP-1 cell line was sub-cloned and one clone (#5) was selected for ability             to differentiate relatively homogeneously in response to PMA. THP-1.5 was used for all             subsequent experiments. THP-1.5 cells were cultured in RPMI, 10% FBS,             Penicillin/Streptomycin, 10mM HEPES, 1mM Sodium Pyruvate, 50uM 2-Mercaptoethanol, and             treated with 30ng/ml PMA (Sigma) to differentiate them into macrophage-like cells.             PMA-differentiated THP-1.5 were stimulated with 10ng/ml LPS (Salmonella Minnesota,             chromatographically purified, Sigma) over a time-course of 24h, in presence of PMA.             Total cell lysates were harvested in TRIzol reagent. Undifferentiated and unstimulated,             PMA-differentiated cells were harvested in TRIzol reagent at the beginning of the LPS             time-course. Total RNA was purified from TRIzol lysates according to manufacturer's             instructions.
THP-1 was stimulated by PMA and harvested after 0h, 2h, 4h, 12h, 24h
tag_length:25-40bp,target_rna_length:10-26bp