PAL_1_1_2008_01_05
marine metagenome
Palmer Station LTER metagenome;archaea
collection_date
2008-01-05T20:30:00-06
biome
seawater
feature
marine_habitat
material
water
geo_loc_name
Polar Antarctic Province
lat_lon
-129.35
rel_to_oxygen
Oxic
samp_collect_device
Niskin
samp_mat_process
NONE
depth
10.6
chlorophyll
4.001 microgramPerLiter
density
27.224
diss_org_carb
45.3 microMoleCarbonPerLiter
diss_oxygen
372.2 microMolePerLiter
elev
0
nitrate
17.7 microMolePerLiter
nitrite
0.3 microMolePerLiter
part_org_carb
531.2 microMolePerLiter
part_org_nitro
121.5 microMolePerLiter
phosphate
1.5 microMolePerLiter
salinity
33.73 PSU
samp_size
1.7 liter
silicate
74.3 microMolePerLiter
temp
0.97 celsius
tot_depth_water_col
1327 meter
tot_nitro
26.4 microMolePerLiter
experimental_factor
seasonal/depth
investigation_type
MIMARKS_Survey
lter_station_name
600.04
ogl_reference_name
PAL_1_1_2008_01_05
sample_type
water
environment
ENVO:seawater;ENVO:marine_habitat;ENVO:water
env_package
water
depth_category
top 50 m
nitrite_plus_nitrate
18 microMolePerLiter
fluorescence
5.7902 milligramPerMeterCubed
leucine
29.74 picoMolePerLiter
carbon_14
29.25 milliMolesCarbonPerMeterSquaredPerDay
bottle
19
cast
1
lib_reads_seqd
5
multiplex_ident_1
CTCTA
nucl_acid_amp
DOI: 10.1126/science.114668
domain
Archaea
pcr_primers
958FWD:AATTGGANTCAACGCCGG;1048REV-major:CGRCGGCCATGCACCWC;1048REV-minor:CGRCRGCCATGYACCWC
target_gene
16S rRNA
target_subfragment
V6
nucl_acid_ext
bead beating alkaline lysis
pcr_cond
initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;28 cycles
sequencing_meth
GS FLX LR70 454 pyrosequencing
url
http://amarallab.mbl.edu/mirada/mirada.html; http://vamps.mbl.edu
bacteria_count
0.204 X106perML
conductivity
2.89 seimenPerMeter
atm_pressure
9.452 db
decimal_time
20.5
shelf_position
ON
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.water