Sample 11
Arabidopsis thaliana
Protocols: Leaves were cut off and directly frozen in liquid nitrogen Arabidopsis thaliana plants were grown from seeds on Jiffy-7 Horticulture peat pellets. Plants were kept under well watered conditions and grown under 12 h of light (80-100 µE) at 23 °C, 12 hours of dark at 20 °C, 70-80% relative humidity for 4 weeks Roots of ten 9-day-old A. thaliana plants were inoculated with 2 mL of L. bicolor cultures (OD600 of 0.1 at 600 nm) in 10 mM MgSO4 buffer. Ten control plants were mock-treated with 2 mL of 10 mM MgSO4 buffer. After additional 19 days, two freshly hatched T. ni larvae were placed on half of the plants of each treatment group (resulting in a two-factorial design with four treatment groups). All plants were covered with mesh nets. Leaves were harvested after 24 h of feeding. RNA was extracted using RNAeasy extraction kit (Qiagen) according to the manufacturer's protocol Libraries were constructed using the TrueSeq RNA Library Prep Kit (Illumina) according to the manufacturer's protocol
ENA-FIRST-PUBLIC
2020-06-01T04:03:50Z
ENA-LAST-UPDATE
2019-11-19T09:48:52Z
External Id
SAMEA6235482
INSDC center name
Georg-August-Universitaet Buesgen-Institut Department of Forest Botany and Tree Physiology
INSDC first public
2020-06-01T04:03:50Z
INSDC last update
2019-11-19T09:48:52Z
INSDC status
public
Submitter Id
E-MTAB-8523:Sample 11
age
28
broker name
ArrayExpress
common name
thale cress
environmental stress
control
genotype
wild type genotype
infect
Laccaria bicolor
organism part
rosette leaf
sample name
E-MTAB-8523:Sample 11
scientific_name
Arabidopsis thaliana
ecotype
Col-0