J000102680_J000107100
Homo sapiens
Protocols: PDX models were generated using subcutaneous tumor implantation in female NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl>/SzJ (NSG; JAX strain 005557) host mice. Tumors were grown, collected, and fragmented for serial passage in NSG mice. Tumor samples at described passage numbers were taken for expression analysis. Tissues preserved in RNAlater were homogenized in TRIzol (ThermoFisher Scientific) using a gentleMACS dissociator (Miltenyi Biotec Inc). Total RNA was isolated using the miRNeasy Mini kit (Qiagen) according to manufacturer‚Äôs protocols, including the optional DNase digest step. RNA quality and concentration were assessed using the RNA 6000 Nano LabChip assay on the 2100 Bioanalyzer instrument and Nanodrop 2000 spectrophotometer (Thermo Scientific). Stranded libraries were prepared using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer's instructions. Briefly, the protocol entails isolation of polyA containing mRNA using oligo-dT magnetic beads, RNA fragmentation, first and second strand cDNA synthesis, ligation of Illumina-specific adapters containing a unique barcode sequence for each library, and PCR amplification. Libraries were checked for quality and concentration using the DNA 1000 assay (Agilent Technologies) and quantitative PCR (KAPA Biosystems), according to the manufacturers' instructions.
ENA-FIRST-PUBLIC
2020-07-01T04:04:31Z
ENA-LAST-UPDATE
2021-09-02T00:21:21Z
External Id
SAMEA6941644
INSDC center name
The Jackson Laboratory
INSDC first public
2020-07-01T04:04:31Z
INSDC last update
2021-09-02T00:21:21Z
INSDC status
public
Submitter Id
E-MTAB-9204:J000102680_J000107100
age
36
broker name
ArrayExpress
common name
human
developmental stage
adult
diagnosis_clinical
colon adenocarcinoma
diagnosis_initial
colon adenocarcinoma
disease
cancer
ethnic group
Not Reported
individual
J000102680
organism part
colon
passage number
P1
primary site
colon
race
White
sample name
E-MTAB-9204:J000102680_J000107100
scientific_name
Homo sapiens
sex
adult
specimen site
liver
treatment naive
No
tumor stage
AJCC IV
tumor type
Metastatic