PAL_9_1_2008_08_15
marine metagenome
Palmer Station LTER metagenome;archaea
multiplex_ident_1
TCGAG
decimal_time
20
elev
0
shelf_position
ON
lib_reads_seqd
11973
ogl_reference_name
PAL_9_1_2008_08_15
domain
Archaea
lat_lon
-129.35
biome
seawater
sequencing_meth
GS FLX LR70 454 pyrosequencing
collection_date
2008-08-15
feature
marine_habitat
sample_type
water
investigation_type
MIMARKS_Survey
environment
ENVO:seawater;ENVO:marine_habitat;ENVO:water
pcr_primers
958FWD:AATTGGANTCAACGCCGG;1048REV-major:CGRCGGCCATGCACCWC;1048REV-minor:CGRCRGCCATGYACCWC
target_gene
16S rRNA
experimental_factor
seasonal/depth
rel_to_oxygen
Oxic
samp_size
2 liter
geo_loc_name
Polar Antarctic Province
depth_category
top 50 m
pcr_cond
initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;28 cycles
material
water
nucl_acid_amp
DOI: 10.1126/science.114668
samp_collect_device
Niskin
lter_station_name
600.04
target_subfragment
V6
env_package
water
url
http://amarallab.mbl.edu/mirada/mirada.html; http://vamps.mbl.edu
samp_mat_process
NONE
depth
10
nucl_acid_ext
bead beating alkaline lysis
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.water