s52a.mms.rna.2
Schizosaccharomyces pombe
Protocols: Cells were collected by filtration. Filters were immediately frozen in liquid nitrogen. Cells were grown for 15 min in 0.02% MMS. Total RNA was extracted using a hot phenol extraction method as described in Lyne et al. (2003) BMC Genomics 4:27. Clean up and DNase treatment were performed on with Purelink RNA microcolumns (Life Technologies) following the manufacturer's instructions. Samples were ribo-depleted using Ribo-Zero Gold rRNA Removal Kit Yeast (Illumina) with a 4 ug input. 30 ng of ribo-depleted RNA was used as starting material for the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific).
ENA-FIRST-PUBLIC
2020-06-01T04:03:53Z
ENA-LAST-UPDATE
2020-01-28T15:59:46Z
External Id
SAMEA6506063
INSDC center name
Department of Biochemistry University of Cambridge UK
INSDC first public
2020-06-01T04:03:53Z
INSDC last update
2020-01-28T15:59:46Z
INSDC status
public
Submitter Id
E-MTAB-8745:s52a.mms.rna.2
broker name
ArrayExpress
common name
fission yeast
fraction
rRNA depleted total RNA
genotype
eIF2alpha-S52A
replicate
2
sample name
E-MTAB-8745:s52a.mms.rna.2
scientific_name
Schizosaccharomyces pombe