Human sample from Homo sapiens
Homo sapiens
Sequences from Guilinger, et al. Nature Methods, 2014.  The specificity profiles of TALENs targeting CCR5B  target sites using an in vitro selection method.  Briefly, preselection libraries of > 10^12 DNA sequences, each theoretically containing at least 10 copies of all possible DNA sequences with six or fewer mutations relative to the on-target sequence (TCTTCATTACACCTGC-random spacer-CATACAGTCAGTATCA), were digested with 40 nM of an in vitro-translated TALEN. Cleaved library members harbored a free 5' monophosphate that enabled them to be captured by adaptor ligation.  We isolated DNA fragments of length corresponding to 1.5 target sites (an intact target site and a repeated half-site up to the point of TALEN-induced DNA cleavage) by gel purification. High-throughput sequencing of TALEN-treated or control samples that survived this selection revealed the abundance of all TALEN-cleaved sequences as well as the abundance of the corresponding sequences before selection.  In the control sample, all members of the preselection library were cleaved by a restriction endonuclease at a constant sequence to enable their capture by adaptor ligation and isolation by gel purification. 
 Selections were indexed with the first 5 bp of sequence.  Thus, the first 5bp of each sequences identifies which selection condition it corresponds to with:
 "NNNNCCTCGGGACTCC" = Pre-selection Library, "CGTAA" = CCR5B L16 + R16, "GTACT" = CCR5B L16 + R13, "TACGA" = CCR5B L16 + R10, "ATGCT" = CCR5B L13 + R16, "TGCAA" = CCR5B L13 + R13, "GCATT" = CCR5B L13 + R10, "GACTA" = CCR5B L10 + R16, "ACTGT" = CCR5B L10 + R13, "CTGAA" = CCR5B L10 + R10
 
isolate
synthetic genomic site
age
NA
biomaterial_provider
IDT
sex
not determined
tissue
NA
BioSampleModel
Human