Untreated biocrust from Avdat site — arid
soil metagenome
This is an arid biocrust which was obtained from Avdat (30°47′ N 34°45′ E) - an elevated hilly region (∼800 m a s l) with mean annual precipitation (P) of ∼90 mm and potential evapotranspiration (PET) of ∼1700 mm yr−1 giving it an aridity index (AIU = P/ P ET ) of 0.053. The soil is an alkaline (pH ∼8.5) calcareous silt-loam
ENA-FIRST-PUBLIC
2013-04-20
ENA-LAST-UPDATE
2012-12-21
External Id
SAMEA1877437
INSDC center alias
MPI-M
INSDC center name
Max Planck Institute for Terrestrial Microbiology
INSDC first public
2013-04-20T17:03:00Z
INSDC last update
2012-12-21T16:13:15Z
INSDC status
public
Submitter Id
A_IS_2
chimera check
UCHIME
collection date
2010-12-20
current land use
Native
depth
0.003
environment (biome)
Arid desert
environment (feature)
Biological soil crust
environment (material)
Soil
environmental package
soil
geographic location (country and/or sea,region)
Israel
geographic location (elevation)
800
geographic location (latitude)
30.47
geographic location (longitude)
34.45
horizon
biocrust
investigation type
mimarks-survey
mean annual and seasonal precipitation
90
miscellaneous parameter
Dry, untreated sample
multiplex identifiers
CTAGTCGA
nucleic acid extraction
http://www.nature.com/protocolexchange/protocols/2484
pH
8.5
pcr primers
343Fmod:TACGGGWGGCWGCA;784Rmod:GGGTMTCTAATCCBKTT
project name
H2O-SIP
relevant standard operating procedures
http://www.mothur.org/wiki/Schloss_SOP
sample name
A_IS_2
sample weight for DNA extraction
0.5
sequencing method
pyrosequncing 454 GS FLX Titanium system
storage conditions (fresh/frozen/other)
Frozen
target gene
Bacterial 16S rRNA
water content of soil
0.0001
water content of soil method
Gravimetric