Generic sample from Zygaena filipendulae
Zygaena filipendulae
L7 larvae were collected from a fallow field in Kvistg*rd north of Copenhagen, Denmark in 2006 by Mika Zagrobelny and reared for a week on L. corniculatus plants devoid of CNglcs. The gut was removed from one larvae and total RNA was extracted from the remaining part of the body using RNeasy plant mini kit from Qiagen (Cat. No. 74904)by Niels Bjerg Jensen. Integrity of the RNA was verified using an Agilent 2100 Bioanalyzer before total RNA was shipped to Eurofins MWG Operon, Inc, Germany (http://www.eurofinsdna.com/home.html). Eurofins MWG Operon performed mRNA purification and normalization in order to thin out the highly abundant transcripts before 454 pyrosequencing. cDNA was produced and fragmented by nebulization and 454 pyrosequencing carried out using a GS FLX  system.