E.coli_03
Caenorhabditis elegans
Protocols: 2000 young adult worms (approximately 50-55h after plating of the L1s) grown on E. coli OP50, B. subtilis PXN21 or a 1:1 mix of E. coli:B. subtilis, were collected and washed three times with M9 + 0.01% Triton X-100 buffer. Three independent biological replicates were used for each experimental condition. The pellet was resuspended in 400 µl of RNA Lysis buffer (Quick-RNA Microprep Kit, Zymo Research) and worms were mechanically disrupted as previously described and kept at -80 °C. Total RNA was extracted from the samples according to the manufacturer's instructions. RNA samples were sent to Edinburgh Genomics for QC check and sequencing. Briefly, quality check of the samples was performed using Qubit with the broad range RNA kit (Thermo Fisher Scientific) and Tapestation 4200 with the RNA Screentape for eukaryotic RNA analysis (Agilent). Libraries were prepared from 5 μg of total RNA using the TruSeq Stranded mRNA kit (Illumina), and then validated. Samples were pooled to create 9 multiplexed DNA libraries.
ENA-FIRST-PUBLIC
2020-01-14T04:04:48Z
ENA-LAST-UPDATE
2019-07-25T10:37:04Z
External Id
SAMEA5815607
INSDC center name
School of Biological Sciences, University of Edinburgh
INSDC first public
2020-01-14T04:04:48Z
INSDC last update
2019-07-25T10:37:04Z
INSDC status
public
Submitter Id
E-MTAB-8164:E.coli_03
age
2
broker name
ArrayExpress
developmental stage
adult
genotype
pkIs2386[Punc-54::-synuclein::YFP + unc-119(+)]
growth condition
Escherichia coli OP50 lawn
organism part
whole organism
sample name
E-MTAB-8164:E.coli_03
scientific_name
Caenorhabditis elegans
sex
hermaphrodite
strain
NL5901