Generic sample from Acropora millepora
Acropora millepora
Larvae from a single cross were reared at 28oC and 32oC for 5 days, until the larvae robustly responded to the settlement cue (crustose coralline algae). Portions of the 28oC culture were then exposed either to the crustose coralline algae, 1 uM GLWamide to induce metamorphosis, or to 32oC, for 4 hours. RNA from larvae from five conditions (28oC culture untreated, exposed to settlement cue, to GLWamide, and to 32oC, plus the culture reared at 32oC) was pooled, normalized using Trimmer kit (Zhulidov et al, 2004), prepared for 454 using a novel method developed in our lab and sequenced.