gut metagenome
Fish specimens were captured using hand nets, kept alive and rapidly transported to a place with semi-sterile conditions to avoid environmental and cross-contamination, taking into account the conditions stated in the collecting permit SGPA/DGVS/02438/16. The complete digestive tract was dissected and preserved in Invitrogen RNAlater Stabilization Solution. All dissected specimens were preserved as vouchers in 95% ethanol and deposited in the Coleccin Nacional de Peces, IBUNAM (Mexico). Genomic DNA was extracted using FastDNA Isolation Kit for Soil (MP Bio) following manufacturers instructions. DNA quality and quantity were measured by both agarose gel electrophoresis and spectrophotometrically with a NanoDrop 2000 (Thermo Fisher Scientific, DE).
collection_date
2018-08
env_biome
ENVO:00002011
env_feature
ENVO:00000067
env_material
UBERON:0000945
geo_loc_name
Mexico: San Luis Potosi, Pachon
host
Astyanax mexicanus
lat_lon
22.587 N 99.037 W
host_sex
female
isolation_source
Pachon_Cavefish_Stomach 2
samp_size
Complete stomach
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.host-associated