20151027_LUHMGU5_e49
Homo sapiens
Protocols: 3 µg of RNA was treated with TURBO DNase, followed by RNeasy Minelute RNA cleanup kit and RNA quaility was confirmed using Agilent 2100 Bioanalyzer with RNA Pico 6000 kit (RIN values >8). 0.5 µg of RNA was used for Lexogen QuantSeq-REV libraries. cDNA libraries were prepared according to Lexogen QuantSeq Rev method manufacturer's protocol, using a poly(T) primer for reverse transcription. The library was sequenced using Illumina HiSeq, producing 60 nt single-end reads and 10 nt index reads H9 human ESCs (H9 line) were cultured in mTeSR1 medium on Matrigel-coated plates. Colonies were passaged for maintanence by Gentle Cell Dissociation Reagent We used shRNA targeting human TDP-43 (GAAACACAAGTGAAAGTAA) or a control targeting firefly luciferase (CGTACGCGGAATACTTCGA) driven by the H1 promoter in the vector FUW coexpressing TagRFP both for transfection and transduction, while maintaining hESC in pluripotency or differentiation growth conditions. Lexogen 3'-end QuandSeq Reverse
ENA first public
2019-02-25
ENA last update
2016-09-20
External Id
SAMEA4444130
INSDC center alias
Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics
INSDC center name
Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics
INSDC first public
2019-02-25T17:03:10Z
INSDC last update
2016-09-20T11:40:39Z
INSDC status
public
Submitter Id
E-MTAB-5090:20151027_LUHMGU5_e49
broker name
ArrayExpress
cell type
KSR differentiated human embryonic stem cell
common name
human
replicate
7
rna interference
shRNA transduction targeting TDP-43
sample name
E-MTAB-5090:20151027_LUHMGU5_e49