Cm
Zindervirus SP6
SP6* was grown at 37C on E. coli K-12 host in LB broth with mutagen (5ug/mL N-methyl-N'nitro nitrosoguanidine) for 38 hours using serial transfer method, then grown in continuous culture without mutagen for 86 hours.  This final evolved population was used to isolate DNA for sequencing.