Input of KKT1
Leishmania major
Leishmania centromeres could not be identified by classical methods including telomere-associated chromosome fragmentation, and position mapping of topoisomerase II binding sites via etoposide-mediated cleavage. Hints for the centromere quest in Leishmania appeared recently with the description of unconventional kinetoplastid kinetochore proteins (KKT) in T. brucei (Akiyoshi and Gull Cell 2015). Chromatin immunoprecipitation coupled to high-throughput sequencing (ChIP-Seq) of L. major KKT1 (LmKKT1) clearly identified one major peak per chromosome. Each peak covers a region of 4 ±2 kb in size. No specific sequence feature could be found, but two grossly conserved motifs were found for 14/36 chromosomes; and a higher density of retroposon was observed in 27/36 centromeres. The identification of centromeres and of a kinetochore component of Leishmania chromosomes provide a tool to further study the composition of this unusual complex this may result in a better understanding of the molecular events leading to chromosomal segregation in this parasite and shed light on the hypothesis that 'permissive' segregation plays a role in mosaic aneuploidy.
ENA first public
2017-09-09
ENA last update
2017-07-10
External Id
SAMEA104157544
INSDC center alias
University of Montpellier, Faculty of Medicine, Dept of Parasitology-Mycology
INSDC center name
University of Montpellier, Faculty of Medicine, Dept of Parasitology-Mycology
INSDC first public
2017-09-09T17:01:29Z
INSDC last update
2017-07-10T18:36:37Z
INSDC status
public
Submitter Id
KKT1-INPUT
sample name
KKT1-INPUT