Generic sample from Caenorhabditis elegans
Caenorhabditis elegans
 (1) embryos were allowed to hatch out and arrest as L1s in S-Basal overnight; (2) the next morning, 10,000 L1s were plated onto 5 large, seeded plates each (50,000 worms total); (3) 3 days later, when worms were gravid adults, they were bleached again to obtain embryos; (4) the embryo sample was divided in half (obtained an average of about 400ul of packed embryos total, those were split up into two 200ul samples of embryos); (5) one ~200ul sample was treated immediately to 3 rounds of freeze/thaw in liquid nitrogen/37C water bath and submitted to RNA isolation protocols; this sample was the early embryo sample. Quality values are Illumina/Solexa quality values (have not been converted to phred) for runs 30KECAAXX, 30DH0AAXX, 30K65AAXX, and 30DEBAAXX). 