Generic sample from Homo sapiens
Homo sapiens
Total small RNAs prepared from HeLa cells 48h post MOCK transfection with Dharmafect4 (Dharmacon). Total RNA was extracted using Trizol (Invitrogen), then short (18-29 nt) RNAs were gel-purified. Library was constructed as described in Seitz et al. (Curr. Biol. 18:147-151 (2008)).