June_ Organic_ Tilled_Clover
soil metagenome
soil
host
not_applicable
isolation_source
agricultural_soil
collection_date
2017-06-01
geo_loc_name
USA: Montana State University Fort Ellis Research and Teaching Center, Bozeman, MT
lat_lon
45.653 N 110.972 W
rel_to_oxygen
anaerobe
samp_collect_device
soil_corer
samp_mat_process
Amplicon libraries of the V4 region of the bacterial 16S rRNA gene were prepared following procedures outlined by Kozich et al. (2013), using dual indexed primers (515F and 806R). Amplicons were normalized using SequalPrep plate normalization kit (Invitrogen), product was pooled to equimolar concentrations, and pooled amplicons were cleaned with AmpureXP magnetic beads . Paired-end sequencing was performed at Michigan State University's RTSF Genomics Core using an Illumina MiSeq with a maximum read length of 250 base pairs on MiSeq v2 reagent cartridge (Illumina Inc.). Illumina Real Time Analysis v1.18.54 was used to perform base calling, and the resulting output was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.19.1.
Cropping_system
Organic_tilled
Crop
Clover
Month
June
pH
5.76
C
3.21
N
0.26
C:N
12.14
Crop_biomass
0.5875
Weed_biomass
53.580125
BioSampleModel
Metagenome or environmental