Human sample from Homo sapiens
Homo sapiens
Sequences from Guilinger, et al. Nature Methods, 2014.  The specificity profiles of TALENs targeting ATM target sites using an in vitro selection method.  Briefly, preselection libraries of > 1012 DNA sequences, each theoretically containing at least 10 copies of all possible DNA sequences with six or fewer mutations relative to the on-target sequence (TGAATTGGGATGCTGTTT-random spacer- TTTATTTTACTGTCTTTA), were digested with 3 nM to 24 nM of an in vitro-translated TALEN. Cleaved library members harbored a free 5' monophosphate that enabled them to be captured by adaptor ligation.  We isolated DNA fragments of length corresponding to 1.5 target sites (an intact target site and a repeated half-site up to the point of TALEN-induced DNA cleavage) by gel purification. High-throughput sequencing of TALEN-treated or control samples that survived this selection revealed the abundance of all TALEN-cleaved sequences as well as the abundance of the corresponding sequences before selection.  In the control sample, all members of the preselection library were cleaved by a restriction endonuclease at a constant sequence to enable their capture by adaptor ligation and isolation by gel purification. 
 Selections were indexed with the first 5 bp of sequence.  Thus, the first 5bp of each sequences identifies which selection condition the sequence originated from.  The indexing is as follows (with selection names corresponding to conditions given in Supplementary Table 1): For sequences in  ATMTALset1_S9 files:"ACTGT" = ATM 28-aa , "CTGAA" = ATM N1, "TGACT" = ATM N2, "TGCAA" = ATM N3, "GCATT" = ATM canonical ELD/KKR, "CATGA" = ATM Q3 ELD/KKR, "ATGCT" = CATM Q7 ELD/KKR, "CTAGT" = ATM N2 ELD/KKR, "CAGTT" = ATM Q3
 For sequences in ATMTALset2_S10 files:  "ACTGT" = ATM 24 nM, "CTGAA" = ATM 12 nM, "TGACT" = ATM 6 nM, "TGCAA" = ATM 3 nM, "GCATT" = ATM 24 nM Q7, "CATGA" = ATM 12 nM Q7, "ATGCT" = ATM 6 nM Q7, "CTAGT" = ATM 3 nM Q7
 Pre-selection library sequences are in ATMLib_S11 files
 
isolate
synthetic genomic site
age
NA
biomaterial_provider
IDT
sex
not determined
tissue
NA
BioSampleModel
Human