Generic sample from Saccharomyces cerevisiae
Saccharomyces cerevisiae
MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 bar1Δ::KanMX (C. Boone, University of Toronto; S288c background derived from BY4741)  Yeast strain and culture conditions S.cerevisiae R276 (MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 bar1Δ::KanMX) (C. Boone, University of Toronto; S288c background derived from BY4741), was cultured overnight in 50 ml rich medium (YPD) at 30*C, diluted into 500 ml fresh YPD to an OD660 of ~0.8, and treated with yeast alpha-factor (Sigma-Aldrich) at a final concentration of 50 ng / ml. The culture was incubated at 30 *C with shaking for 3 hours (final OD660 ~ 1).  After this treatment, approximately 90% of the cells had formed mating projections when checked by light microscopy.