Sideroxydans sp. CL21_S.oneidensis_coculture1
mixed sample
Protocols: Samples (50 mL) were collected from (1) Sideroxydans sp. CL21 monocultures (n=3), (2) S. oneidensis monocultures (n=3), and (3) Sideroxydans sp. CL21 + S. oneidensis co-cultures (n=3) via centrifugation (5000 x g, 10 min, 4 degC) and cell pellets were used for nucleic acid extraction. Total RNA was extracted using a phenol-chloroform extraction method adapted from Wegner, et al 2019. 5ng of enriched mRNA was used for cDNA library preparation (NEBNext Ultra II Directional RNA Library Prep kit for Illumina; New england Biolabs, MA, USA) and purified with Ampure XP Beads (New England Biolabs, MA, USA). cDNA libraries were quantified by chip-based gel electrophoresis using an Agilent Bioanalyzer 2100 and the Agilent DNA7500 kit (Agilent, Waldbronn, DE). Equimolarly pooled cDNA libraries were sequenced using a Illumina NextSeq 500 platform
ENA-FIRST-PUBLIC
2020-06-01T04:03:57Z
ENA-LAST-UPDATE
2020-04-30T10:03:20Z
External Id
SAMEA6809875
INSDC center name
Institute of Biodiversity Friedrich Schiller University Jena
INSDC first public
2020-06-01T04:03:57Z
INSDC last update
2020-04-30T10:03:20Z
INSDC status
public
Submitter Id
E-MTAB-9015:Sideroxydans sp. CL21_S.oneidensis_coculture1
broker name
ArrayExpress
growth condition
co-culture of Sideroxydans sp. CL21 and Shewanella oneidensis MR-1
sample name
E-MTAB-9015:Sideroxydans sp. CL21_S.oneidensis_coculture1
scientific_name
mixed sample
species mix
Shewanella oneidensis MR-1; Sideroxydans sp. CL21