Generic sample from Clostridium difficile 6534
Clostridioides difficile 6534
Clostridium difficile is a gram positive spore forming pathogen with a wide host range. Microarray based comparative genome hybridizations (Janvilisri et al, J Bacteriol. 2009 Jun;191(12):3881-91) have shown that the core genome of this species could be as low as 16% of the total genome. The objective of this project is to perform comparative genome sequencing of new C. difficile isolates and compare the new genomes to the reference strain C. difficile 630 (Genbank ID: AM180355).  Based on microarray based comparative genome hybridizations of 80 C. difficile isolates from different hosts and geographical areas, strains showing large degree of genome variation was selected for shot gun sequencing. Strain 6534 sequenced in this study is a non-epidemic bovine isolate and will be used for comparative sequence analysis.
strain
6534