Generic sample from Pinaceae
Pinaceae
Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.  barcode ATT Picea sitchensis PICSIT04   barcode CCT Pinus longaeva LONG01   barcode GGT Pinus monophylla MONO10   barcode AAT Pinus nelsonii NELS03
barcode ATT
Picea sitchensis PICSIT04
barcode CCT
Pinus longaeva LONG01
barcode GGT
Pinus monophylla MONO10
barcode AAT
Pinus nelsonii NELS03