Sequences of CLTA, EMX and VEGF on- and off-target genomic sites from Hek293T cells treated with Control-wild typ Cas9and control sgRNAs
Homo sapiens
Sequencing from Guilinger, Thompson & Liu. Nature Biotechnology 2014.  The target site and its corresponding known off-target sites of CLTA, EMX, and VEGF were amplified from genomic DNA isolated from HEK293 cells treated with wild-type Cas9 and two sgRNAs targeting an unrelated site (GFP) as a negative control.  All genomic target sites (for CLTA, EMX, and VEGF) were combined together forthis one type of Cas9 treatment.
sex
female
tissue
Hek293
isolate
Hek293
age
?
biomaterial_provider
GenTarget
Cas9 expression vector
wild typ Cas9 nuclease
gRNA targets
GFP
Cas9 expression vector (ng)
700
gRNAs expression vector (ng)
250
genomic sites amplifed
CLTA, EMX and VEGF
on-target sites
CLT2_On;EMX_On;VEG_On
off-target sites
CLT2_Off1-3;EMX_Off1-4;VEG_Off1-4
BioSampleModel
Human