ShCtrlBDNF16h-1
Rattus norvegicus
Protocols: Hippocampi from E18 rat embryos brain were collected, treated with trypsin, and plated on poly-D-lysine (Sigma) coated 12-well plates at a density of 3 x 105 /well. Neurons were maintained in Neurobasal medium (Invitrogen) supplemented with 2% B-27 (Invitrogen) and 0.5 mM glutamine (Invitrogen). Half of the culture medium was changed every 3 days after plating. For RNA-seq, lenti-shATF4 and lenti-shCtrl viruses were used to infect cultured hippocampal neurons on 7 DIV; cells were treated with BDNF on 12 DIV; RNA samples were collected at indicated time points. Total RNA was isolated at indicated time points after BDNF treatment by using RNeasy Mini Kit (Qiagen). Reverse transcription was performed by using the First-strand cDNA Synthesis Kit (Origene) following the manufacturer's instructions. TruSeq Stranded Total RNA LT Sample Prep Kit
ENA first public
2017-09-09
ENA last update
2017-08-30
External Id
SAMEA104230580
INSDC center alias
DarwinHealth, Inc
INSDC center name
DarwinHealth, Inc
INSDC first public
2017-09-09T17:01:30Z
INSDC last update
2017-08-30T09:51:14Z
INSDC status
public
Submitter Id
E-MTAB-6046:ShCtrlBDNF16h-1
broker name
ArrayExpress
cell type
neuron
common name
Norway rat
developmental stage
embryonic day 18
organism part
Ammon's horn
replicate
1
sample name
E-MTAB-6046:ShCtrlBDNF16h-1
stimulus
brain derived neurotrophic factor