Tus_BRCA1del_siBLM_Rep2
Mus musculus
source_name
Mouse embryonic stem (ES) cells
genotype
BRCA1del/exon11
sirna
BLM
reporter
4kb 1xGFP cassette in ROSA26
nuclease delivery
Transfection; Tus/nuclease/siRNA
treatment protocol
48 hour culture post transfection
bait dsb
Cas9/US30kb_ROSA26 gRNA
growth protocol
MEF feeder; ES medium