Alternative splicing and trans-splicing events revealed by analysis of Bombyx mori transcriptome
Bombyx mori
To gain a global view of the silkworm transcriptome, we collected 77 samples from the whole life cycle of B. mori, including 18 from embryo, 29 from larva, 26 from pupa and 4 from adult, in which female and male were equally considered. Total RNA from each sample were equally mixed together and the ribosomal RNAs were removed by Ribominus method, then the RNA sample was applied to Illumina Genome Analyser 2 for sequencing.