U-343 MGa
Homo sapiens
Protocols: Cells are seed in the plate until 80% confluence. Then cells were washed by PBS twice, and lysed with the lysis buffer. Cells were maintained in Minimum Essential Medium (MEM) with10% FBS and penicillin-streptomycin at 37°C with 5% CO2 and over 95% humidify. Total RNA was purified using the PureLink® kit following the manufacturer's instructions (Thermo Fisher Scientific) Illumina TruSeq strand-specific RNA libraries (poly-A selection)
ENA-FIRST-PUBLIC
2020-06-01T04:03:52Z
ENA-LAST-UPDATE
2019-12-18T16:13:06Z
External Id
SAMEA6428704
INSDC center name
Department of Oncology-pathology, Karolinska Institutet
INSDC first public
2020-06-01T04:03:52Z
INSDC last update
2019-12-18T16:13:06Z
INSDC status
public
Submitter Id
E-MTAB-8620:U-343 MGa
broker name
ArrayExpress
cell line
U-343 MGa
common name
human
disease
glioblastoma
organism part
brain
phenotype
GFAP+FN1-
sample name
E-MTAB-8620:U-343 MGa
scientific_name
Homo sapiens