Generic sample from Pinus monticola
Pinus monticola
Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
barcode ATGT
Pinus monticola MONT26E1
barcode CGTT
Pinus monticola MONT30E2
barcode CCCT
Pinus monticola MONT36E2
barcode GTAT
Pinus monticola MONT38E1
barcode TACT
Pinus monticola MONT49E1