Mus musculus
strain
129
dev_stage
E12.5
sex
not applicable
tissue
embryonic fibroblasts
cell_type
mouse embryonic fibroblasts
growth_protocol
Early passage (passage 1) mouse embryonic fibroblasts (MEFs) were cultured for 48hr in MEF medium (GMEM medium supplemented with 10% FCS, 1 mM sodium pyruvate, 1 mM glutamine, and 5.7 ml MEM 100X Non-Essential Amino Acids Solution, 50 M 2-mercaptoethanol (Gibco, Thermo Fisher Scientific) at 37C and 5% CO2. MEFs were seeded onto 15-cm dishes and cultured for 24hr in MEF medium before being infected with Tet-on-O, -S, -K, -M and rtTA2M2 lentiviruses at a multiplicity of infection (M.O.I) of 5 for each virus. OSKM expression was induced by treating infected cells with 1 g/mL Dox for 48hr.
sample_type
cell culture
BioSampleModel
Model organism or animal