jai_2
Solanum lycopersicum
Protocols: Flowers of wild type, jai1-1 and myb21-2 were harvested in a very strict time window of 30 min starting at 7 h after the onset of light period, carpels dissected, transferred to liquid nitrogen and stored at -80 C. Plants were grown in a controlled growth chamber with 16 h light (110 µmol photons*m-2*s-1) at 26°C and 8 h dark at 20°C, both at 70 % humidity. RNA isolation from homogenized material (ball mill) was performed using the RNeasy Plant Mini Kit (Qiagen) according to the supplier's instructions followed by routine DNase treatment using DNA-freeTM Kit (Thermo Scientific). RNA quality was tested by capillary electrophoresis using QIAxcel Advanced System (Qiagen). A one μg subsample of total RNA from each of 9 RNA extracts (1 stage×3 genotypes×3 biological replicates) were sent to the GATC Biotech AG (Konstanz, Germany, https://www.eurofinsgenomics.eu/) and used to produce Illumina HiSeq 2500 strand-specific 2x150 bp paired-end reads.
ENA-FIRST-PUBLIC
2019-11-05T17:04:32Z
ENA-LAST-UPDATE
2018-12-19T16:38:11Z
External Id
SAMEA5190798
INSDC center name
Leibniz Institute of Plant Biochemistry Department of Cell and Metabolic Biology
INSDC first public
2019-11-05T17:04:32Z
INSDC last update
2018-12-19T16:38:11Z
INSDC status
public
Submitter Id
E-MTAB-7545:jai_2
age
5
broker name
ArrayExpress
common name
tomato
cultivar
Micro-Tom
developmental stage
flower development stage 3
genotype
jai1-1
organism part
carpel
sample name
E-MTAB-7545:jai_2
scientific_name
Solanum lycopersicum