Sample 1
Homo sapiens
Protocols: Nascent RNA was captured using 45min pulse according to the manual to Click-iT® Nascent RNA Capture Kit. 1 µg of DNAse-treated RNA was treated with RiboZero Gold (Human/Mouse/Rat) kit to remove rRNAs, followed by RNA cleanup using the RNeasy Minelute RNA cleanup kit. Sequencing libraries were prepared using TruSeq Stranded total RNA LT kit according to manufacturer's instructions using 11 cycles of PCR followed by purification with Agencourt Ampure XP beads. Libraries were evaluated on an Agilent 2100 Bioanalyzer using the DNA 1000 kit and DNA concentration was measured using a Qubit dsDNA HS Assay Kit. Samples were sequenced using a HiSeq2500 instrument to generate 50-nt single-end reads, sequencing depth was 20-40 Mio reads per library. H9 human ESCs (H9 line) were cultured in mTeSR1 medium on Matrigel-coated plates. Colonies were passaged for maintanence by Gentle Cell Dissociation Reagent Human ESCs were not treated and maintained in pluripotency using mTesr medium under same conditions and in parallel as samples prepared for total RNAseq. RNA-seq
ENA first public
2019-02-25
ENA last update
2016-10-03
External Id
SAMEA4476483
INSDC center alias
Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics
INSDC center name
Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics
INSDC first public
2019-02-25T17:03:11Z
INSDC last update
2016-10-03T16:41:35Z
INSDC status
public
Submitter Id
E-MTAB-5115:Sample 1
broker name
ArrayExpress
cell type
undifferentiated human embryonic stem cell
common name
human
replicate
1
sample name
E-MTAB-5115:Sample 1