R1
Drosophila melanogaster
Protocols: For pcm[14] rescue males the UAS-pcm[WT] construct was first crossed into the pcm[14] stock to generate y[1] pcm[14]/FM7i-GFP; UAS-pcm[WT]/CyO-GFP stock. Virgin females from this stock were then crossed to males of arm-GAL4 driver. For wild-type E1-E3 is clean excision of the P element (Bloomington stock 11456) used to create pcm[14]. For wild-type G1-G3 is arm-Gal4 parent. For wild-type U1-U3 is UAS parent. 60 testes were dissected per biological replicate for wild-type samples. 120 testes were dissected per replicate for pcm[14] rescue sample. RNA was extracted using a Qiagen miRNeasy micro kit (217084) with on-column DNase treatment (79254) Sequencing libraries were created using an Illumina TruSeq RNA Sample Prep Kit v2
ENA first public
2020-06-01
ENA last update
2016-03-03
External Id
SAMEA3886627
INSDC center alias
Brighton and Sussex Medical School
INSDC center name
Brighton and Sussex Medical School
INSDC first public
2020-06-01T04:05:06Z
INSDC last update
2016-03-03T14:09:41Z
INSDC status
public
Submitter Id
E-MTAB-4515:R1
broker name
ArrayExpress
common name
fruit fly
developmental stage
2-3 days old males
genetic modification
pcm null
genotype
w[1118], pcm[14]/+; UAS-pcm[WT]/+; arm-Gal4/+
organism part
testis
sample name
E-MTAB-4515:R1