SL1344 + PABN replicate 3
Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Protocols: S. Typhimurium SL1344 was grown to an OD600 0.6 under shaking incubation at 37 oC. The growth media was MOPS minimal medium supplemented with 0.4g/l histidine. 3 ml bacterial cultures were spun down and frozen for later use. S. Typhimurium SL1344 was grown to an OD600 0.6 under shaking incubation at 37 oC. The growth media was MOPS minimal medium supplemented with 0.4g/l histidine. Samples were treated for two hours with the known efflux inhibitor phe-arg-beta-napthylamide (100 µg/ml) or the suspected efflux inhibitor chlorpromazine (50 µg/ml) Total RNA was extracted from frozen bacterial pellets obtained from 3 ml of culture. The Promega SV Total RNA Isolation kit was used according to the manufacturers instructions. RNA dilution buffer was added and the samples were heated for 3 minutes at 70 oC then centrifuged for 10 minutes. The RNA was then eluted in water and the sample treated with an RNAse inhibitor. The library was constructed by BGI, Hong Kong. The rRNA was depleted with a Ribo-Zero Magnetic kit. cDNA was generated with First Strand MasterMix and SuperScript II. Second Strand MasterMix was then used. cDNA was combined with End RepairMix and purified with Ampure XP Beads. Poly-A tails were added with an A-TailingMix.
ENA-FIRST-PUBLIC
2020-06-01T04:05:43Z
ENA-LAST-UPDATE
2019-07-26T11:01:35Z
External Id
SAMEA5816037
INSDC center name
University of Birmingham
INSDC first public
2020-06-01T04:05:43Z
INSDC last update
2019-07-26T11:01:35Z
INSDC status
public
Submitter Id
E-MTAB-8190:SL1344 + PABN replicate 3
broker name
ArrayExpress
genotype
wild type genotype
growth condition
cell suspension culture
replicate
3
sample name
E-MTAB-8190:SL1344 + PABN replicate 3
scientific_name
Salmonella enterica subsp. enterica serovar Typhimurium
strain
SL1344
serovar
Typhimurium