PAL_8_2_2008_01_27
marine metagenome
Palmer Station LTER metagenome;archaea
collection_date
2008-01-27
biome
seawater
feature
marine_habitat
material
water
geo_loc_name
Polar Antarctic Province
lat_lon
-137.52
rel_to_oxygen
Oxic
samp_collect_device
Niskin
samp_mat_process
NONE
depth
99.821
chlorophyll
0.076 microgramPerLiter
density
27.368
diss_org_carb
45.5 microMoleCarbonPerLiter
diss_oxygen
329 microMolePerLiter
elev
0
nitrate
20.1 microMolePerLiter
nitrite
0.2 microMolePerLiter
part_org_carb
NULL
part_org_nitro
NULL
phosphate
2 microMolePerLiter
salinity
34.0317 PSU
samp_size
2 liter
silicate
71.9 microMolePerLiter
temp
-1.0168 celsius
tot_depth_water_col
170 meter
tot_nitro
32 microMolePerLiter
experimental_factor
seasonal/depth
investigation_type
MIMARKS_Survey
lter_station_name
200.-020
ogl_reference_name
PAL_8_2_2008_01_27
sample_type
water
environment
ENVO:seawater;ENVO:marine_habitat;ENVO:water
env_package
water
depth_category
top 100 m
nitrite_plus_nitrate
20.3 microMolePerLiter
fluorescence
0.06 milligramPerMeterCubed
leucine
NULL
carbon_14
73.7 milliMolesCarbonPerMeterSquaredPerDay
bottle
5
cast
69
lib_reads_seqd
3950
multiplex_ident_1
CAGTA
nucl_acid_amp
DOI: 10.1126/science.114668
domain
Archaea
pcr_primers
958FWD:AATTGGANTCAACGCCGG;1048REV-major:CGRCGGCCATGCACCWC;1048REV-minor:CGRCRGCCATGYACCWC
target_gene
16S rRNA
target_subfragment
V6
nucl_acid_ext
bead beating alkaline lysis
pcr_cond
initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;28 cycles
sequencing_meth
GS FLX LR70 454 pyrosequencing
url
http://amarallab.mbl.edu/mirada/mirada.html; http://vamps.mbl.edu
bacteria_count
0.247 X106perML
conductivity
2.75 seimenPerMeter
atm_pressure
99.768 db
decimal_time
20.4
shelf_position
ON
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.water