Fet_Org_W8_repl3
Homo sapiens
source_name
Cultured organoid from fetal stomach tissue (PCW 8)
passage
P 10
culture method
Split at single cells with TrypLE; plated in 30 uL droplets of Matrigel Growth Factor Reduced; expanded for 8 days in gastric culture medium WENR (wnt, egf, noggin, r-spondin); isolated from Matrigel using 20 min of ice cold Cell Release Solution ECM digestion; RNA extracted in RLT buffer.