Generic sample from Homo sapiens
Homo sapiens
RNA from three pooled preparations of MonoMac-6 cells, resuspended in 250 uL PBS and isolated with Trizol LS. A library of 19-33 nucleotide RNA was prepared according to the methods of Pfeffer S, Lagos-Quintana M, Tuschl T. Curr Protoc Mol Biol. 2005 Nov;Chapter 26:Unit 26.4. Cloning of Small RNA Molecules. RNA isolation, library preparation and 454 technology sequencing was performed in parallel with the library for exosome-like vesicles. The adaptors used for library preparation were as follows: 5' (454 A primer - identifying tag - 5' adaptor; GCCTCCCTCGCGCCATCAG-GTCA-GAATTCCTCACTAAA-cloned RNA) and 3'(cloned RNA - 3'adaptor - 454 B primer; TTTAACCGCGAATTC-CTGAGCGGGCTGGCAAGGC).