Generic sample from Caenorhabditis elegans
Caenorhabditis elegans
 Animals were synchronized by treating a mixed population with bleach to collect embryos.  The embryos were incubated without food, causing the larvae to arrest at the L1 stage upon hatching.  Starved L1 larvae were plated in the presence of food and raised at 25 degrees Celsius. Staging was determined by examining a subset of animals under Nomarski optics at intervals for vulval and germline development.  Animals were collected at mid-YA stage and washed in S basal buffer.  Exact times for growth after plating of starved L1s (all at 25 degrees C) were as follows:  Young Adult for 46 hours (vulvae fully formed and oocytes  present in gonad, but no embryos).  