PAL_5_2_2008_01_23
marine metagenome
Palmer Station LTER metagenome;archaea
collection_date
2008-01-23
biome
seawater
feature
marine_habitat
material
water
geo_loc_name
Polar Antarctic Province
lat_lon
-139.48
rel_to_oxygen
Oxic
samp_collect_device
Niskin
samp_mat_process
NONE
depth
11.857
chlorophyll
0.17 microgramPerLiter
density
27.107
diss_org_carb
43.7 microMoleCarbonPerLiter
diss_oxygen
344.7 microMolePerLiter
elev
0
nitrate
20.2 microMolePerLiter
nitrite
0.6 microMolePerLiter
part_org_carb
62.9 microMolePerLiter
part_org_nitro
13.5 microMolePerLiter
phosphate
1.6 microMolePerLiter
salinity
33.8298 PSU
samp_size
2 liter
silicate
39 microMolePerLiter
temp
0.9155 celsius
tot_depth_water_col
3701 meter
tot_nitro
30.6 microMolePerLiter
experimental_factor
seasonal/depth
investigation_type
MIMARKS_Survey
lter_station_name
200.2
ogl_reference_name
PAL_5_2_2008_01_23
sample_type
water
environment
ENVO:seawater;ENVO:marine_habitat;ENVO:water
env_package
water
depth_category
top 50 m
nitrite_plus_nitrate
20.8 microMolePerLiter
fluorescence
0.5256 milligramPerMeterCubed
leucine
4.94 picoMolePerLiter
carbon_14
10.08 milliMolesCarbonPerMeterSquaredPerDay
bottle
20
cast
56
lib_reads_seqd
7
multiplex_ident_1
TACTA
nucl_acid_amp
DOI: 10.1126/science.114668
domain
Archaea
pcr_primers
958FWD:AATTGGANTCAACGCCGG;1048REV-major:CGRCGGCCATGCACCWC;1048REV-minor:CGRCRGCCATGYACCWC
target_gene
16S rRNA
target_subfragment
V6
nucl_acid_ext
bead beating alkaline lysis
pcr_cond
initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;28 cycles
sequencing_meth
GS FLX LR70 454 pyrosequencing
url
http://amarallab.mbl.edu/mirada/mirada.html; http://vamps.mbl.edu
bacteria_count
0.251 X106perML
conductivity
2.89 seimenPerMeter
atm_pressure
11.975 db
decimal_time
4.25
shelf_position
OFF
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.water