br910-clr4-WCE
Schizosaccharomyces pombe
Protocols: Log-phase yeast cells were incubated at 18°C for 2 hours and then fixed for 30 minutes in 3% freshly made formaldehyde. The crosslinking reaction was stopped by the addition of 2.5M Glycine to make a final concentration of 0.125M. The cells was pelleted and with PBS (phosphate buffered saline) before resuspended in ChIP lysis buffer (50 mM HEPES-KOH, pH7.5, 140 mM NaCl, 1% Triton X-100, 0.1% Deoxycholat supplemented with cOmplete protease inhibitors cocktail (Roche)). Ice cold glass beads were added and the mixtures were vigorously disrupted in a beadbeater. The lysates were collected and subjected to sonication to reduce chromatin size to 500-1000 base pairs. The protein DNA mixture were then subjected to proteinase K treatment and phenol:chloroform extraction before the DNA was precipitated by ethanol. DNA samples were prepared according to TurSeq ChIP sample preparation guide (Illumina)
ENA first public
2016-10-03
ENA last update
2016-09-29
External Id
SAMEA4471221
INSDC center alias
Columbia University in the City of New York
INSDC center name
Columbia University in the City of New York
INSDC first public
2016-10-03T17:01:37Z
INSDC last update
2016-09-29T12:15:17Z
INSDC status
public
Submitter Id
E-MTAB-4776:br910-clr4-WCE
broker name
ArrayExpress
common name
fission yeast
genotype
clr4 mutant
sample name
E-MTAB-4776:br910-clr4-WCE