PAL_2_1_2008_01_05
marine metagenome
Palmer Station LTER metagenome;archaea
collection date
2008-01-05T20:30:00-06
biome
seawater
feature
marine_habitat
material
water
geographic location
Polar Antarctic Province
geographic location (latitude and longitude)
-129.35
relationship to oxygen
Oxic
sample collection device
Niskin
sample material processing
NONE
depth
99.9
chlorophyll
0.058 microgramPerLiter
density
27.52
dissolved organic carbon
40.8 microMoleCarbonPerLiter
dissolved oxygen
253.8 microMolePerLiter
elevation
0
nitrate
27.2 microMolePerLiter
nitrite
0.2 microMolePerLiter
particulate organic carbon
NULL
particulate organic nitrogen
NULL
phosphate
2.2 microMolePerLiter
salinity
34.2672 PSU
sample size
1.5 liter
silicate
84 microMolePerLiter
temperature
-0.09 celsius
total depth of water column
1327 meter
total nitrogen
36.6 microMolePerLiter
experimental factor
seasonal/depth
investigation type
MIMARKS_Survey
lter_station_name
600.04
ogl_reference_name
PAL_2_1_2008_01_05
sample type
water
environment
ENVO:seawater;ENVO:marine_habitat;ENVO:water
environmental package
water
depth_category
top 100 m
nitrite_plus_nitrate
27.4 microMolePerLiter
fluorescence
0.0678 milligramPerMeterCubed
leucine
NULL
carbon_14
29.25 milliMolesCarbonPerMeterSquaredPerDay
bottle
9
cast
1
lib_reads_seqd
1005
multiplex_ident_1
ACGCA
nucl_acid_amp
DOI: 10.1126/science.114668
domain
Archaea
pcr_primers
958FWD:AATTGGANTCAACGCCGG;1048REV-major:CGRCGGCCATGCACCWC;1048REV-minor:CGRCRGCCATGYACCWC
target_gene
16S rRNA
target_subfragment
V6
nucl_acid_ext
bead beating alkaline lysis
pcr_cond
initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;28 cycles
sequencing_meth
GS FLX LR70 454 pyrosequencing
url
http://amarallab.mbl.edu/mirada/mirada.html; http://vamps.mbl.edu
bacteria_count
0.155 X106perML
conductivity
2.84 seimenPerMeter
atm_pressure
101.264 db
decimal_time
20.5
shelf_position
ON
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.water