TIA1_iCLIP_LUd4_AG
Homo sapiens
Protocols: HeLa cells grown in a 10 cm plate. Medium was replaced with ice-cold PBS buffer and cells were subjected to UV-C irradiation (100 mJ/cm2, Stratalinker 2400). Cells were then precipitated by centrifugation for 1 min at 14000 rpm and snap-frozen on dry ice. iCLIP or iCLAP protocol was followed to isolate RNA bound to TIA1 and TIAL1 proteins and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction, and are specified below for each sequencing run. Random barcodes were also incoperated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina pair-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
ENA first public
2011-07-29
ENA last update
2018-03-08
External Id
SAMEA680935
INSDC center alias
CRI
INSDC center name
Cancer Research UK Cambridge Research Institute
INSDC first public
2011-07-29T17:00:39Z
INSDC last update
2018-03-08T15:27:56Z
INSDC status
public
Submitter Id
E-MTAB-432:TIA1_iCLIP_LUd4_AG
broker name
ArrayExpress
cell line
HeLa
common name
human
sample name
E-MTAB-432:TIA1_iCLIP_LUd4_AG
sex
female