metagenome
isolation_source
King Penguin cloaca
collection_date
2015-12-22
geo_loc_name
South Georgia and the South Sandwich Islands: St. Andrew's Bay
lat_lon
54.433 S 36.183 W
samp_collect_device
Regular flocked swabs (Eswab, Copan, Murrieta, CA, USA) were used for cloacal swabs as part of gastrointestinal microbial and viral analysis from penguins. The swabs were stored in 1 ml of liquid Amies medium.
samp_mat_process
Viral DNA was extracted from 200 l of the Amies medium suspension after vortexing with the High Pure Viral Nucleic Acid Kit (Roche Diagnostics, Indianapolis, IN, USA). The resulting viral DNA extraction was subjected to rolling circle amplification (RCA) using the TempliPhi 100 Amplification Kit (GE Healthcare, Chicago, IL, USA). An aliquot of the resulting RCA-amplified DNA (5 l) was pooled and used to generate Illumina sequencing libraries using the Nextera DNA Flex Library Prep Kit (Illumina Inc, San Diego, CA, USA). The libraries were sequenced on an Illumina 4000 sequencer (2 100 bp library)
samp_size
20: (adults=10; chicks=10)
BioSampleModel
Metagenome or environmental