Generic sample from freshwater metagenome
freshwater metagenome
A mix of 90 clones from the environmental 16S rRNA clone library from Priest Pot. This mixture contained potentially similar sequences at concentrations varying over several orders of magnitude and was used to test the ability of our algorithms to remove noise and allow accurate OTU determination. The V5/V6 region was from the 90 clones (that had also been Sanger sequenced) was Pyrosequenced using GSFLX.