Generic sample from Homo sapiens
Homo sapiens
RNA from three pooled preparations of exosome-like vesicles prepared by differential centrifugation from the supernatant of MonoMac-6 cells. Exosome-like vesicles were resuspended in 250 uL PBS and isolated with Trizol LS. A library of 19-33 nucleotide RNA was prepared according to the methods of Pfeffer S, Lagos-Quintana M, Tuschl T. Curr Protoc Mol Biol. 2005 Nov;Chapter 26:Unit 26.4. Cloning of Small RNA Molecules. RNA isolation, library preparation and 454 technology sequencing was performed in parallel with the library for total cell RNA. The adaptors used for library preparation were as follows: 5' (454 A primer - identifying tag - 5' adaptor; GCCTCCCTCGCGCCATCAG-GATC-GAATTCCTCACTAAA-cloned RNA) and 3'(cloned RNA - 3'adaptor - 454 B primer; TTTAACCGCGAATTC-CTGAGCGGGCTGGCAAGGC).