Caenorhabditis elegans mutant strain with two ASER chemosensory neurons
Caenorhabditis elegans
C Elegans mutant strain with two ASER chemosensory neurons lsy-12 (ot177). Caenorhabditis elegans mutant allele identification by whole-genome sequencing. Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.