Pinus chloroplast genomes
Pinus
Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
barcode AGCT
Pinus armandii ARMA04
barcode CCCT
Pinus parviflora var. pentaphylla PARV14
barcode CTGT
Pinus sibirica SIBI02
barcode TACT
Pinus squamata SQUA02
barcode GGGT
Pinus strobus STRO27
barcode ACGT
Pinus wallichiana WALL02