T15
Homo sapiens
Protocols: Whole blood was collected into PAXgene tubes (Qiagen, Valencia, CA). Written informed consent for the use of patient material was provided. Ethical approval was granted by the NRES Committee Leeds (East) (Ref: 07/Q1206/7). Samples were collected and RNAseq undertaken at 10 months (T14), 11 months(T15), 14 months (T16), 16 months (T17), 18 months and 11 days (T18), 18 months and 12 days (T19), 18 months and 14 days (T20), 18 months and 23 days (T21), and 20 months (T22). Treatment was commenced at 17 months of age with Dexamethasome at a dose of 10mg/m2. Subsequently at 18 months and 11 days of age Ruxolitinib was added at 2.5mg twice a day after the RNA sample was collected on that day. Samples were collected and RNAseq undertaken at 10 months (T14), 11 months (T15), 14 months (T16), 16 months (T17), 18 months and 11 days (T18), 18 months and 12 days (T19), 18 months and 14 days (T20), 18 months and 23 days (T21), and 20 months (T22). Total RNA extracted using a PreAnalytix RNA isolation kit. RNA integrity was analysed on an Agilent 2100 Bioanalyzer. Illumina Truseq stranded mRNA Assay. Paired end.
ENA-FIRST-PUBLIC
2019-08-22T04:02:42Z
ENA-LAST-UPDATE
2018-10-09T10:30:04Z
External Id
SAMEA4964511
INSDC center name
UNIVERSITY OF MANCHESTER
INSDC first public
2019-08-22T04:02:42Z
INSDC last update
2018-10-09T10:30:04Z
INSDC status
public
Submitter Id
E-MTAB-7275:T15
age
11
broker name
ArrayExpress
common name
human
disease
interferonopathy
individual
patient1
organism part
blood
sample name
E-MTAB-7275:T15
scientific_name
Homo sapiens
sex
not available