Generic sample from Pinus
Pinus
Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
barcode AGCT
Pinus albicaulis ALBI05
barcode ACGT
Pinus aristata ARIS02
barcode CCCT
Pinus ayacahuite AYAC01
barcode CTGT
Pinus cembra CEMB03
barcode GGGT
Pinus flexilis FLEX13
barcode TACT
Pinus peuce PEUC03