Generic sample from Pinaceae
Pinaceae
Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
barcode AATT
Picea sitchensis PICSIT04
barcode ATT
Pinus lambertiana N LAMB08
barcode CTGT
Pinus monticola MONT05
barcode TACT
Pinus rzedowskii RZED01
barcode ACGT
Pinus torreyana ssp. insularis T0RR03