2lox_Esrrb_2i
Mus musculus
Protocols: ES cells were cultured on 0.1% gelatin-coated plates in 2i/LIF conditions. Cells were fixed using 1% formaldehyde for 10 min at room temperature and fixation quenched with 150 mM glycine. Chromatin was sheared to an average fragment size between 200 and 300 bp using a Bioruptor sonication instrument (Diagenode). Immunoprecipitation was performed using antibodies against endogenous proteins or the FLAG tag. Libraries were prepared with the NEXTflex Rapid Illumina DNA-seq Library Prep Kit (Bioo Scientific).
ENA first public
2016-08-09
ENA last update
2016-03-16
External Id
SAMEA3902978
INSDC center alias
University of Cambridge
INSDC center name
University of Cambridge
INSDC first public
2016-08-09T17:01:30Z
INSDC last update
2016-03-16T14:08:40Z
INSDC status
public
Submitter Id
E-MTAB-4565:2lox_Esrrb_2i
broker name
ArrayExpress
cell line
2lox
cell type
embryonic stem cell
common name
house mouse
genotype
Mbd3flox/-
sample name
E-MTAB-4565:2lox_Esrrb_2i