Candida albicans
Protocols: Candida albicans cells (wild type strain SN95 [HSP90/HSP90], and mutant strain CalC1441 [tetO-HSP90/hsp90]) were grown in YPD (2% yeast extract, 2% bactopeptone, 2% glucose) to mid-exponential phase. Cultures were grown either in the absence or presence of 20 g/ml doxycycline. Cells were then exposed to a 30 C-42 C heat shock, and RNA extracted 10, 30 and 60 minutes post-heat shock. Untreated controls were included, for which RNA was extracted at time zero before heat shock. Biological triplicate cultures were processed. Cells were flash frozen in liquid nitrogen and RNA was extracted using Qiazol (phenol/chloroform method). RNA was DNase treated using Ambion Turbo DNAfree kit before being sent for library preparation and sequencing Libraries were prepared using standard Illumina RNAseq library preparation kit.