Ixodes ricinus
Engorged nymphs of ticks (Ixodes ricinus) originally sampled on roe deer, then molted into female adults in the lab, were used for this expression study. Malpighian tubules of 5 partially fed individuals were collected (4 days after deposit on the host). This is the replicate 1 out of 3 biological replicates of the D4_C_MT condition. Librairies were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina and were sequenced on Novaseq S4 PE150 using the NovaSeq 6000 S4 Reagent Kit.