Ixodes ricinus
Engorged nymphs of ticks (Ixodes ricinus) originally sampled on roe deer, then molted into female adults in the lab, were used for this expression study. Malpighian tubules of 5 unfed individuals were collected. This is the replicate 1 out of 3 biological replicates of the D0_C_MT condition. Librairies were prepared using the Clontech Smart-seq v4 ultra low kit and were sequenced on Novaseq S4 PE150 using the NovaSeq 6000 S4 Reagent Kit.