Mus musculus
Protocols: PatchSeq - extraction of the cell content (whole cell body) after patch-clamp whole-cell recording. Physical extraction of each cell contents onto lysis buffer containing C1-P1-T31 (5'-AAT GAT ACG GCG ACC ACC GAT CGT31 -3') primer. Full-length cDNA libraries were tagmented and amplified with Nextera XT DNA library preparation kit . Smart-seq2 protocol used for single cell sequencing