Homo sapiens
Protocols: LK-2 cells were cultured in RPMI (gibco) supplemented with 10% fetal bovine serum and 1% Penicillin/Streptomycin. ChIP-Seq was done exactly as described in (Schmidt, D., et al. ChIP-seq: Using high-throughput sequencing to discover protein-DNA interactions. Methods 48, 240-248 (2009)). Briefly 2 x 15cm plates per cell line were formaldehyde crosslinked, nuclear fraction was isolated and chromatin sonicated using Bioruptor Pico (Diagenode). Libraries were produced at the Wellcome Trust Sanger Institute sequencing facility. Each library was split into two and run on separate lanes