Ixodes ricinus
Ticks were sampled by flagging the vegetation, on a single site. A pool of 50 nymphs of Ixodes ricinus were collected. Ticks were washed in a solution of chlorine, rinsed in pure water and sent in a tube of RNAlater to Claude Rispe, in the lab of Nantes, INRAE-Oniris. The RNA of each sample (pool of 50 whole bodies) was extracted. Libraries were made with poly-A selection and a stranded protocol. Transcriptomes were sequenced on one lane of an Illumina Hiseq 3000 machine.