Homo sapiens
Protocols: All tissues were shipped overnight in Belzer's solution at 4 degrees Celsius and were processed and cultured within 4 hours of obtaining the specimen. Experiments to evaluate the effect on progenitor maintenance in culture by media conditions were repeated using tissues from 3 individual lung specimens; (1) 84 day post fertilization of unknown gender, (2), 87 day post fertilization male, and (3), 87 day post fertilization of unknown gender. RNAseq experiments of whole lung homogenate utilized tissue from 3 additional individual lungs; (4) 59 day male (5) 87 day of unknown gender and (6) 125 day of unknown gender. Use of normal adult human lung tissue was approved by University of Michigan institutional review board, and was obtained from warm autopsy organ donors arranged by the Gift of Life, Michigan. RNA was isolated using the mirVana RNA isolation kit, following the “Total RNA” isolation protocol (Thermo-Fisher Scientific, Waltham MA). RNA sequencing library preparation and sequencing was carried out by the University of Michigan DNA Sequencing Core and Genomic Analysis Services (https://seqcore.brcf.med.umich.edu/).