Solanum tuberosum
Due to the small size of the tubers, 3 tubers were used for each treatment x time-point to ensure enough tissue for RNA extraction. RNA was extracted using a modified hot phenol protocol.The Ambion Turbo DNA-free system (Thermo Fischer Scientific, Waltham, MA) was used to remove genomic DNA.  RNA quality was analyzed using an Agilent bioanalyzer (Agilent Technologies, Santa Clara, CA).  Illumina cDNA libraries were prepared using the TruSeq DNA sample prep kit and sequenced in single-end mode (50nt) on the Illumina Hiseq 2000 platform (Illumina, San Diego, CA).