Drosophila melanogaster
CS flies were reared at room temperature (23-24C) for collection.  Whole body samples were homogenized in 1ml of Trizol (Invitrogen, Carlsbad, California, USA) per 50-100 mg of tissue using a glass-Teflon homogenizer.  RNA was purified according to the suggested manufacturer's protocol (Invitrogen). Eggs were laid on grape juice plates for 2 hours and then removed and aged for 14 more hours.