Aliger gigas
Total RNA was isolated from 12 individual conch tissue samples (neural ganglia, gonad, digestive gland from each of four conchs) using TRIzol (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol; 200 ng each RNA sample were pooled and used as the template for the cDNA library construction and normalization using the SMART (Clontech/Takara Bio USA, Madison, WI, USA) and Trimmer (Evrogen Joint Stock Company, Moscow, Russia) kits, respectively.