Homo sapiens
Protocols: MCF7 cells were maintained in DMEM supplemented with 10% (v/v) FBS, penicillin (100 U/ml), streptomycin (100mg/ml) (Life Technologies). Incubation conditions were 37 C , 5% CO2 MCF-7 cells grown in 0.1% hypoxic conditions, after 72hrs the cells were sorted according to whether they expressed CA9 (CA9 +) or not (CA9-). Total RNA extraction at 72 hrs