Saccharomyces cerevisiae
Protocols: Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate (Sigma-Aldrich) in the presence of 0.8 M sorbitol (Sigma-Aldrich). After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation. Total RNA was isolated using the RNeasyMini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove any contaminating genomic DNA. Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina).