Mus musculus
Protocols: Non-infected (naive) mice were sacrificed and perfused transcardially with pre-warmed heparinized saline (50 mL PBS with 250 l heparine per animal) using a peristaltic pump. Gonadal adipose tissue was collected and immediately lysed using TRIzol reagent. Total RNA was extracted using TRIzol reagent according to the manufacturers instructions (Life Technologies). Total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturers instructions (Invitrogen). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina).