Brucella abortus
Protocols: Two flasks containing 10 mls of fresh Brucella Broth were (each one) inoculated with 2 mls of an overnight culture of Brucella abortus 2308. Erythritol was added at 1 mg/ml to one of them and both cultures were then allowed to grow during 2.5 h until an OD600 = 0.6-0.7 RNA was purified by using the Qiagen RNeasy Protect bacteria mini kit (Quiagen, USA)and RNase free DNase I digestion. Pure RNA (5 ug) was depleted of rRNA with the Ambion MICROBExpress kit.