Mus musculus
Protocols: Murine lung endothelial cell line (L2 MVEC) was seeded in 6 well plates at the concentration of 5*10^4 cells/mL. After expanding the cells for 24 hours, they were washed with medium and stimulated for 24 hours with combinations of murine IFN-gamma (20 ng/mL) and dexamethasone (100 nM). RNA was extracted with RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. Purity of RNA was determined by NanoDrop and quality was assesed with RIN measurement (value of 10 for all samples). Per independent experiment RNA from 3 technical replicates per experimental condition was pooled and 3μg RNA/experimental condition were sequenced from a total of 3 independent experiments. Illumina TruSeq stranded mRNA kit was used and the single-end sequencing was performed. 50bp reads were sequenced.