Ciona intestinalis
Embryos exhibiting upregulation of Zic-r.b and Erk signaling and downregulation of Foxa.a, Foxd (adnZE) were incubated in seawater containing 2.5 ?g/mL cytochalasin B, an inhibitior of actin polymerization, from fertilized egg. Sampling was performed at the early tailud equivalent stage, that is, when control embryos reached the early tailbud stage