Homo sapiens
Protocols: Single cell FACS of tetramer-enriched PBMCs Single tetramer+ cells were sorted into 96-well plates containing 0.5 μl dNTP mix (10 mM), 0.5 μl oligo-dT pri- mer (5 μM), and 1 μl lysis buffer (prepared by adding 1 μl RNase inhibitor to 19 μl Triton X-100 solution, 0.2% v/v). Libraries were prepared using the Smart-seq2 protocol with the following modifications. cDNA synthesis and pre-amplification reaction volumes were halved to 5 and 12.5 μl, respectively. The final concentration of the ERCC RNA Spike-In Mix in the reverse-transcription step was 1:400 million.