Homo sapiens
Protocols: To retrieve cells for RNA isolation, hydrogels were degraded in 0.5 mg ml-1 collagenase A (11088793001; Sigma-Aldrich) at 37°C for 60-90 min. MSCs from 3 different donors were encapsulated (1.5 Mio ml-1) within 1.7% TG-PEG hydrogels and cultured in medium containing MEM alpha, FCS (10%), penicillin (100 U ml-1), streptomycin (100 µg ml-1) and human recombinant FGF-2 (0 or 50 ng ml-1) at 37°C in a humidified atmosphere at 5% CO2 for 7 days. Medium was exchanged after 4 days. Total RNA was isolated from cells using the RNeasy Micro Kit following the manufacturer's instructions (74004; QIAGEN). 200 ng total RNA was used for RNA-seq library prep with the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to manufacturer's specifications.