marine metagenome
Seawater was collected from the DCM layer (50 m deep) off the coast of Alicante, Spain with a Niskin bottle on October 15, 2007. Total DNA was isolated from the pico-planktonic 5-0.2 micrometer size fraction. A fosmid library was created from this DNA (ca. 13000 fosmids) and a part of the DNA from this sample was also sequenced using directly pyrosequencing.