Hibiscus cannabinus
A SSH library of mesta against MeYVMV infection. Mesta plants were raised from HC-583 seeds under glasshouse condition and whitefly transmission was carried out with 11 days old plants. Total RNA were isolated from collected leaves of infected plants as well as of control plants after 12 hr, 24 hr, 2 and 10 dpi using Trizol reagent (invitrogen). mRNA was isolated by mRNA isolation kit (Roche Applied Science). The differentially regulated subtracted cDNA pool in healthy plants were obtained using the Clontech PCR select Subtraction Kit. This subtracted cDNA pool were then cloned in pJET vector and transformed in DH5-alpha cells and thus differentially regulated cDNA library was constructed.