Gesta propertius
Adult females were collected from 4 populations of E. propertius,  near the latitudinal center of the species distribution (within 50 km of Medford, Oregon). Eggs   laid by these females were hatched under conditions characteristic of native habitats in a greenhouse and then moved to Conviron growth chambers at the University of Notre Dame. Multiple individuals of each larval instar were collected through the final instar before pupation. Individuals of the 2nd, 3rd, and 4th instars  were exposed to a heat stress of 35 degrees   for 60 minutes and a cold stress of 0 degrees for 120 minutes. Individuals in the 5th and 6th instar of    were exposed to a desiccation agent (silica gel) for   120 minutes. After treatment, larvae were  frozen in liquid nitrogen and stored at -80 degrees C.    Whole-body RNA from these frozen individuals was extracted using an RNA Easy kit   (QIAGEN Inc.) over a period of two months. Prior to library construction, pooling   was done by adjusting sample contributions to equimolar amounts of total RNA per individual.  In total, 53 larvae of were pooled prior to sequencing, representing a minimum of 11 and a maximum of 48 wild   mothers.