Phaeodactylum tricornutum
Protocols: P.tricornutum cells from three biological replicates were harvested in the middle of light period by vacuum filtration on 1.2 um polycarbonate filters (Millipore, United States) long time(8 months). P. tricornutum cells were harvested in the middle of the light period by vacuum filtration onto 1.2 um polycarbonate filters (Millipore, United States), then flash frozen in liquid N2 and maintained at -80C until RNA extraction. The LC sample comprised three biological replicates and HC sample comprised three biological replicates. P.tricornutum cells from three biological replicates were harvested in the middle of light period by vacuum filtration on 1.2 um polycarbonate filters (Millipore, United States) long time(8 months). P. tricornutum cells were harvested in the middle of the light period by vacuum filtration onto 1.2 um polycarbonate filters (Millipore, United States), then flash frozen in liquid N2 and maintained at -80C until RNA extraction. The LC sample comprised three biological replicates and HC sample comprised three biological replicates. Total RNA was extracted from the frozen cell pellets using the TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer's protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) 7 or higher were subjected to the subsequent analysis. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.
ENA-FIRST-PUBLIC
2019-11-07T17:04:19Z
ENA-LAST-UPDATE
2019-09-20T11:47:30Z
External Id
SAMEA5789185
INSDC center name
Marine Life College, Ocean University of China
INSDC first public
2019-11-07T17:04:19Z
INSDC last update
2019-09-20T11:47:30Z
INSDC status
public
Submitter Id
E-MTAB-8351:Sample HC3
broker name
ArrayExpress
genotype
wild type genotype
growth condition
1000 ppmv CO2 environment
organism part
whole organism
sample name
E-MTAB-8351:Sample HC3
scientific_name
Phaeodactylum tricornutum
strain
NEPCC640
time
8