Drosophila melanogaster
Bosch et al. 2020 figure
Fig. 1F
Biological replicate
1
FACS enrichment for GFP+
Yes
PE2 source
NA
pegRNA/sgRNA source
pCFD5-PE3-ebony[G111X]
Condition
Sort
Amplicon
ebony
Replicate
rep1
Sample type
Transfected S2R+ cells
Amplicon target
ebony
strain
S2R+/PT5 cells
age
not applicable
sex
not applicable
tissue
S2R+/PT5 cells
biomaterial_provider
Justin Bosch
genotype
pegRNA plasmid transfection into Act-PE2 stable PT5/S2R+ cells
Temperature raised
25˚C
pegRNA spacer
CTGGCCATCTGGAAGGCTGG
pegRNA extension
GGCAAATACGCGCtttaAGCCTTCCAGATGG
nicking spacer
GCTTCGCCTCCAGCAGTATG
Forward primer
CCGGTTCCTGCAGCCAAACA
Reverse primer
GGGATTTGGCATACAGTTCG
WT amplicon
CCGGTTCCTGCAGCCAAACAGCGATGGTGACTTCATCGTGGCTGTGTGCATGCAGCCGTCGGAGGGATTGGTCACCACACTGCTGGCCATCTGGAAGGCTGGCGGCGCGTATTTGCCCATCGATCCCAGCTTCCCGGCGAACCGCATTCACCACATACTGCTGGAGGCGAAGCCCACCTTGGTGATTCGCGACGATGACATCGACGCCGGCCGTTTCCAGGGAACTCCCACGTTATCCACCACCGAACTGTATGCCAAATCCC
Edited amplicon
CCGGTTCCTGCAGCCAAACAGCGATGGTGACTTCATCGTGGCTGTGTGCATGCAGCCGTCGGAGGGATTGGTCACCACACTGCTGGCCATCTGGAAGGCTTAAAGCGCGTATTTGCCCATCGATCCCAGCTTCCCGGCGAACCGCATTCACCACATACTGCTGGAGGCGAAGCCCACCTTGGTGATTCGCGACGATGACATCGACGCCGGCCGTTTCCAGGGAACTCCCACGTTATCCACCACCGAACTGTATGCCAAATCCC
BioSampleModel
Model organism or animal