Mus musculus
ale and or female mice were intra-gastrically gavaged daily with either vehicle control or thiopurine drugs (TG or MP). Caecal content (CC), caecal mucosa (CM) and faeces (F) of WT, Hprt-/- and Winnie mice were collected at day 14 post-TG administration at doses of 0, 0.5 mg/kg. DNA was extracted from faeces at day 0 and day 14; CM and CC at day 14. DNA extracts were used as the template for PCR-based amplification of the bacterial V1-V3 region of the 16S rRNA gene using a barcoded pyrotagging approach, and sequenced with a Roche 454 FLX+ with titanium chemistry. ID type time strain treatment 1 faecal d0 Winnie C 2 faecal d0 Winnie C 3 faecal d0 Winnie C 4 faecal d0 Winnie C 5 faecal d0 Winnie C 6 faecal d0 Winnie C 7 faecal d0 Winnie T 8 faecal d0 Winnie T 9 faecal d0 Winnie T 10 faecal d0 Winnie T 11 faecal d0 Winnie T 12 faecal d0 Winnie T 13 faecal d0 HPRT C 14 faecal d0 HPRT C 15 faecal d0 HPRT C 16 faecal d0 HPRT T 17 faecal d0 HPRT T 18 faecal d0 HPRT T 19 faecal d0 HPRT T 20 faecal d0 HPRT C 21 faecal d0 HPRT C 22 faecal d0 HPRT C 23 faecal d0 HPRT T 24 faecal d0 HPRT T 25 faecal d0 C57 C 26 faecal d0 C57 C 27 faecal d0 C57 C 28 faecal d0 C57 T 29 faecal d0 C57 T 30 faecal d0 C57 T 31 faecal d0 C57 T 32 faecal d0 C57 C 33 faecal d0 C57 C 34 faecal d0 C57 C 35 faecal d0 C57 T 36 faecal d0 C57 T 37 faecal d14 Winnie C 38 faecal d14 Winnie C 39 faecal d14 Winnie C 40 faecal d14 Winnie C 41 faecal d14 Winnie C 42 faecal d14 Winnie C 43 faecal d14 Winnie T 44 faecal d14 Winnie T 45 faecal d14 Winnie T 46 faecal d14 Winnie T 47 faecal d14 Winnie T 48 faecal d14 Winnie T 49 faecal d14 HPRT C 50 faecal d14 HPRT C 51 faecal d14 HPRT C 52 faecal d14 HPRT C 53 faecal d14 HPRT C 54 faecal d14 HPRT C 55 faecal d14 HPRT T 56 faecal d14 HPRT T 57 faecal d14 HPRT T 58 faecal d14 HPRT T 59 faecal d14 HPRT T 60 faecal d14 HPRT T 61 faecal d14 C57 C 62 faecal d14 C57 C 63 faecal d14 C57 C 64 faecal d14 C57 C 65 faecal d14 C57 C 66 faecal d14 C57 C 67 faecal d14 C57 T 68 faecal d14 C57 T 69 faecal d14 C57 T 70 faecal d14 C57 T 71 faecal d14 C57 T 72 faecal d14 C57 T 73 caecal d14 Winnie C 74 caecal d14 Winnie C 75 caecal d14 Winnie C 76 caecal d14 Winnie C 77 caecal d14 Winnie C 78 caecal d14 Winnie C 79 caecal d14 Winnie T 80 caecal d14 Winnie T 81 caecal d14 Winnie T 82 caecal d14 Winnie T 83 caecal d14 Winnie T 84 caecal d14 Winnie T 85 caecal d14 HPRT C 86 caecal d14 HPRT C 87 caecal d14 HPRT C 88 caecal d14 HPRT C 89 caecal d14 HPRT C 90 caecal d14 HPRT C 91 caecal d14 HPRT T 92 caecal d14 HPRT T 93 caecal d14 HPRT T 94 caecal d14 HPRT T 95 caecal d14 HPRT T 96 caecal d14 HPRT T 97 caecal d14 C57 C 98 caecal d14 C57 C 99 caecal d14 C57 C 100 caecal d14 C57 C 101 caecal d14 C57 C 102 caecal d14 C57 C 103 caecal d14 C57 T 104 caecal d14 C57 T 105 caecal d14 C57 T 106 caecal d14 C57 T 107 caecal d14 C57 T 108 caecal d14 C57 T 109 mucosal d14 Winnie C 110 mucosal d14 Winnie C 111 mucosal d14 Winnie C 112 mucosal d14 Winnie C 113 mucosal d14 Winnie C 114 mucosal d14 Winnie C 115 mucosal d14 Winnie T 116 mucosal d14 Winnie T 117 mucosal d14 Winnie T 118 mucosal d14 Winnie T 119 mucosal d14 Winnie T 120 mucosal d14 Winnie T 121 mucosal d14 HPRT C 122 mucosal d14 HPRT C 123 mucosal d14 HPRT C 124 mucosal d14 HPRT C 125 mucosal d14 HPRT C 126 mucosal d14 HPRT C 127 mucosal d14 HPRT T 128 mucosal d14 HPRT T 129 mucosal d14 HPRT T 130 mucosal d14 HPRT T 131 mucosal d14 HPRT T 132 mucosal d14 HPRT T 133 mucosal d14 C57 C 134 mucosal d14 C57 C 135 mucosal d14 C57 C 136 mucosal d14 C57 C 137 mucosal d14 C57 C 138 mucosal d14 C57 C 139 mucosal d14 C57 T 140 mucosal d14 C57 T 141 mucosal d14 C57 T 142 mucosal d14 C57 T 143 mucosal d14 C57 T 144 mucosal d14 C57 T
ENA first public
2015-09-30
ENA last update
2015-09-01
External Id
SAMEA3518228
INSDC center alias
CBMSO
INSDC center name
Centro Biologia Molecular Severo Ochoa (CBMSO)
INSDC first public
2015-09-30T17:24:36Z
INSDC last update
2015-09-01T11:52:07Z
INSDC status
public
Submitter Id
thio1b
common name
house mouse
sample name
thio1b