Homo sapiens
Protocols: The analysis was performed on total RNA from two clones each of HepG2WTΔT1, ΔT2, +T3, and two pools of HepG2WT cells, Total RNA was extracted from exponentially growing cells using RNeasy®kit (Qiagen) Library was constructed using Illumina Truseq RNA Sample Preparation Kit. High quality total RNA was DNase I treated, mRNA isolated by magnetic bead Oligo (dT) enrichment, followed by mRNA fragmentation, cDNA synthesis, single nucleotide A (adenine) addition, and adapter ligation.
ENA-FIRST-PUBLIC
2015-09-30T17:24:36Z
ENA-LAST-UPDATE
2018-03-09T03:11:23Z
External Id
SAMEA3535269
INSDC center name
Copenhagen Center for Glycomics, University of Copenhagen
INSDC first public
2015-09-30T17:24:36Z
INSDC last update
2018-03-09T03:11:23Z
INSDC status
public
Submitter Id
E-MTAB-3844:GALNT3KI_2
broker name
ArrayExpress
cell line
HepG2
common name
human
genetic modification
gene knock in
genotype
GALNT3 knock-in
sample name
E-MTAB-3844:GALNT3KI_2
scientific_name
Homo sapiens