Mus musculus
Protocols: doi:10.1016/j.ymeth.2009.03.001; Shortly, liver material was crosslinked in 1% formaldehyde for 20 minutes followed by a 10 minute incubation in 500 mM glycine buffer to neutralize the formaldehyde. Samples were rinsed with PBS and frozen until use. Human material was obtained from Addenbrooke¹s Hospital, Cambridge licence (08/H0308/117) and the Liver Tissue Distribution Program at the University of Pittsburgh (NIDDK Contract # N01-DK-9-2310). Formaldehyde-crosslinked/flash frozen tissue was stored at ­80C at the CRI. Tc1 mice (annotated as Tc1_with_hschr21) were generated as described in (O¹Doherty et al., 2005). Mice were initially obtained from Victor Tybulewic and Elizabeth Fisher and subsequently housed and bred within the Biological Resources Unit at CRI. Tc0 mice (annotated as Tc1_without_hschr21) are Tc1 sibling mice (ie the same genetic background) that have not inherited a copy of HsChr21.
ENA-FIRST-PUBLIC
2013-05-28T10:10:16Z
ENA-LAST-UPDATE
2018-03-08T15:45:58Z
External Id
SAMEA1905293
INSDC center name
Cancer Research UK
INSDC first public
2013-05-28T10:10:16Z
INSDC last update
2018-03-08T15:45:58Z
INSDC status
public
Submitter Id
E-MTAB-1104:tc1_137.1
age
2-6 month
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
Tc1_with_hschr21
individual
tc1_137.1
organism part
liver
sample name
E-MTAB-1104:tc1_137.1
scientific_name
Mus musculus
sex
male