Plasmodium berghei
Protocols: Male Tcr delta-deficient mice (Tcrdtm1Mom) (TCRdelta-/-) and wild-type C57BL/6 mice, of ages 6-8 weeks old, were infected with intravenous (i.v.) inoculation of 2x10^4 GFP-expressing P. berghei ANKA sporozoites purified from mosquito salivary glands. Parasites were collected from the blood at exactly 74 hours after sporozoites infection, at the late trophozoite stage of development. Samples were pooled from 3 mice/ group. DNA was removed with a TURBO DNA-free Kit (Applied Biosystems).​ Barcoded stranded mRNA-seq libraries were prepared using the Illumina TruSeq kit implemented on the liquid handling robot Beckman FXP2.
ENA-FIRST-PUBLIC
2019-04-11T17:04:03Z
ENA-LAST-UPDATE
2019-03-11T11:36:09Z
External Id
SAMEA5410849
INSDC center name
Instituto Gulbenkian de Ciencia, Oeiras, Portugal
INSDC first public
2019-04-11T17:04:03Z
INSDC last update
2019-03-11T11:36:09Z
INSDC status
public
Submitter Id
E-MTAB-6493:WT1
broker name
ArrayExpress
genotype
wild type genotype
growth condition
Collected from WT mice blood
sample name
E-MTAB-6493:WT1
scientific_name
Plasmodium berghei
strain
ANKA