Mus musculus
Protocols: MIP-EGFP mice [B6.Cg-Tg(Ins1-EGFP)1Hara/J] were crossed with CD1 females to obtain embryo or mice of desired ages. The presence of vaginal plug at noon was considered as E0.5. E16.5 pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. Cell purity was >98% in all samples.
ENA-FIRST-PUBLIC
2015-10-01T17:02:40Z
ENA-LAST-UPDATE
2018-03-08T23:41:08Z
External Id
SAMEA3276301
INSDC center name
Vanderbilt University
INSDC first public
2015-10-01T17:02:40Z
INSDC last update
2018-03-08T23:41:08Z
INSDC status
public
Submitter Id
E-MTAB-3351:GG67 pool of 6 embryos
age
E16.5
broker name
ArrayExpress
common name
house mouse
sample name
E-MTAB-3351:GG67 pool of 6 embryos
scientific_name
Mus musculus
strain
STOCK Tg(Tnpo1-EGFP)6729Hara/Mmmh