Homo sapiens
source_name
MCF10A
cell type
MCF10A
treatment
5 µM Palbociclib for 48 hours to arrest cells in G1; 3 µg/mL doxycycline added for last 24 hours to induce Cas9D10A
in-situ treatment
1.8 U of S1 nuclease @15 mins on ice, followed by 37 degrees C for 20 mins.
harvested day
N/A