Acanthamoeba castellanii
This sample was used for a sequence-based exploration of transgene integration in Acanthamoeba castellanii. Acanthamoeba castellanii strain Neff (ATCC-30010) cells were transformed with plasmid pGAPDH-EGFP and maintained in PYG medium under selection with G418. No clones were isolated following transformation; the sequence data come from a mixed population of multiple successfully transformed cells. Genomic DNA from the cultures grown from these mixed transformants were sequenced with the Oxford Nanopore MinION.
strain
Neff (ATCC-30010)
collection_date
1957
env_biome
N/A
env_feature
N/A
env_material
soil
estimated_size
About 42 000 000 bp
geo_loc_name
USA: Pacific Grove, California
isol_growth_condt
Axenic growth in PYG-like medium
lat_lon
36.62 N 121.92 W
num_replicons
N/A
ploidy
unknown
propagation
eukaryote: unknown sexuality
isolation_source
soil
BioSampleModel
MIGS.eu