Homo sapiens
Protocols: Collection of clinical samples by surgery. Fresh tissues were dissected and immediately submerged in 5 volumes of RNAlater Stabilization Solution (Cat No. AM7020, Thermofisher Scientific, Waltham, MA), incubated at 4°C overnight and then transferred to -20°C. RNA was extracted from one fetal lung and seven urinary bladder samples by using TissueLyser LT (Cat No. 85600, QIAGEN, Hilden, Germany) and RNeasy Fibrous Tissue Mini Kit (cat. no. 74704, QIAGEN, Hilden, Germany). The RNA was measured both quantitatively and qualitatively using NanoDrop (Thermofisher Scientific, Waltham, MA) and Bioanalyzer 2100 (Agilent, Santa Clara, CA) instruments. Illumina TruSeq mRNA library protocol according to standard procedures.
ENA first public
2017-11-30
ENA last update
2016-10-10
External Id
SAMEA4501262
INSDC center alias
Karolinska Institutet, Department of Women's and Children's Health
INSDC center name
Karolinska Institutet, Department of Women's and Children's Health
INSDC first public
2017-11-30T17:02:44Z
INSDC last update
2016-10-10T16:25:48Z
INSDC status
public
Submitter Id
E-MTAB-5143:Sample 3
age
10
broker name
ArrayExpress
common name
human
organism part
urinary bladder
sample name
E-MTAB-5143:Sample 3