Mus musculus
Protocols: All mice were of C57BL/6J genetic background and housed in the animal facility of the Institute of Molecular Genetics of the ASCR v.v.i. under SPF conditions. 3-6 week-old animals were used. Thymi were enzymaticaly digested, cells were MACS enriched for CD45- fraction and FACS sorted using BD Influx sorter. mTECs-high were gated as EpCAM+CD11c-Ly51-MHCII+CD80+ cells and sorted into RPMI media (Sigma) containing 10% FSC and Penicillin/Streptomycin (Gibco). Cells were then cultured in a 96 flat-well plate in 200 uL of 10% FSC RPMI with Penicilin/Streptomycin in the presence of Endotoxin-free TLR9 ligand-CpG ODN (ODN 1826) (InvivoGen) at a concentration of 5 uM or without the ligand. After 24 hours, the supernatant was removed, cells were resuspended in RNA-lysis buffer. RNA was extracted using a RNeasy Plus Micro Kit (Qiagen). RNA-seq libraries were prepared by EMBL Gene core facility in Heidelberg.
ENA-FIRST-PUBLIC
2020-03-20T17:05:11Z
ENA-LAST-UPDATE
2019-06-10T11:40:11Z
External Id
SAMEA5702960
INSDC center name
Laboratory of Genomics and Bioinformatics Institute of Molecular Genetics of the ASCR
INSDC first public
2020-03-20T17:05:11Z
INSDC last update
2019-06-10T11:40:11Z
INSDC status
public
Submitter Id
E-MTAB-8025:KO_NO3
age
3 to 6
broker name
ArrayExpress
common name
house mouse
developmental stage
premature
genotype
MyD88 knockout
individual
KO_NO3
organism part
thymus
organism part
mTEC
sample name
E-MTAB-8025:KO_NO3
scientific_name
Mus musculus
sex
mixed
stimulus
not stimulated
strain
C57BL/6J