Bacillus subtilis subsp. subtilis
Protocols: To initiate sporulation, cells grown overnight on solid DSM medium7 (Schaeffer et al., 1965) were inoculated in preheated liquid DSM at OD600 0.05 and incubated at 37°C with shaking of 200 rpm until the cells leave exponential growth, which time point was considered as sporulation initiation point and taken as T0 (or Age 0), 3 hour after sporulation initiation time point T0 was taken as T3 (or Age 3). Cells were harvested from sporulation induced samples at time point T0 and T3. Total RNA extraction was performed through RNeasy Mini Kit (Qiagen). After removing rRNA from total RNA samples through Ribo-Zero rRNA Removal Kit (Bacteria) (illumina), library was prepared through TruSeq mRNA stranded Bacteris HT.
ENA-FIRST-PUBLIC
2020-03-18T04:05:22Z
ENA-LAST-UPDATE
2019-07-05T16:26:32Z
External Id
SAMEA5759988
INSDC center name
Systems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
INSDC first public
2020-03-18T04:05:22Z
INSDC last update
2019-07-05T16:26:32Z
INSDC status
public
Submitter Id
E-MTAB-8124:Sample 18
broker name
ArrayExpress
developmental stage
sporulation
genotype
wild type genotype
sample name
E-MTAB-8124:Sample 18
scientific_name
Bacillus subtilis subsp. subtilis
strain
168
type-material
type strain of Bacillus subtilis