Ceratitis capitata
The Mediterranean fruit fly Ceratitis capitata (Wiedemann), commonly known as the medfly, is a destructive agricultural pest and the object of expensive population eradication and suppression efforts within state and federal departments of agriculture. Area-wide integrated pest management programs control medfly populations through the release of sterile males which must be massively produced. Mass-rearing and release of sterile males is facilitated by two sex-linked traits white pupae (wp) and temperature sensitive lethal (tsl). Though these two sex-linked traits in what is known as a genetic sexing strain was developed over 20 years ago, the genetic basis of wp is unknown. To map wp, three mapping populations were generated from an F4 wp strain and wild type lab line strain cross. Three sibships with individuals segregating for the white pupae trait were then genotyped by sequencing (GBS) and identifying SNPs from a restriction site associated DNA library. The SNP genotypes were then used to identify chromosome-scale linkage groups. Loci linked to the wp locus were identified using quantitative trait loci (QTL) analysis. GBS sequences for additional colony flies along with wild-caught flies were also obtained, and this data has been used to develop a genetic assay for differentiating between recaptured sterile insect released males and wild males.
isolate
Mapping population
isolation_source
Mass-reared laboratory colonies and wild
collection_date
2013
geo_loc_name
not applicable
tissue
Whole body
dev_stage
Adult
sex
pooled male and female
specimen_voucher
GBS plates 1,2,3
BioSampleModel
Invertebrate