Arabidopsis thaliana
Protocols: Unopened flower buds at floral stage 9 were collected in tubes, snap frozen in liquid nitrogen and stored at -80°C until further use. Plants were grown under long day conditions (16 hours light/8 hours dark) at 20°C. 6 μg of total RNA was treated with DNAseI using TURBO DNase reagents (ThermoFisher, AM2238). 4 μg of total DNAseI-treated total RNA was then treated with Ribo-Zero rRNA removal magnetic kit (Epicentre, MRZPL116) to deplete rRNA from the total RNA sample. 100 mg of unopened flower buds were ground in liquid nitrogen and RNA was extracted using Trizol reagent. 50 ng of rRNA-depleted DNAseI-treated RNA was used to prepare a stranded RNA-seq library using the ScriptSeq v2 RNA-seq Library Preparation Kit (Epicentre, SSV21124), as described in Choi et al. (2018) Genome Res. 28(4): 532-546.
ENA-FIRST-PUBLIC
2019-11-10T04:03:12Z
ENA-LAST-UPDATE
2018-11-01T14:13:45Z
External Id
SAMEA5059739
INSDC center name
Department of Plant Sciences, University of Cambridge
INSDC first public
2019-11-10T04:03:12Z
INSDC last update
2018-11-01T14:13:45Z
INSDC status
public
Submitter Id
E-MTAB-7371:wt_RNAseq_Rep1
age
6
broker name
ArrayExpress
common name
thale cress
developmental stage
floral organ formation stage
ecotype
Col-0
genotype
wild type genotype
growth condition
long day length regimen
organism part
flower bud
sample name
E-MTAB-7371:wt_RNAseq_Rep1
scientific_name
Arabidopsis thaliana