Homo sapiens
Protocols: Freshly collected tissues (6 cancers and matched normal mucosa samples) were processed to investigate their methylome. For methylation analysis, 1µg DNA was used for library preparation according to the Roche SeqCapEpi CpGiant protocol (Roche, Rotkreutz, Switzerland; details in Supplementary Experimental Procedures). Specifically with this method, DNA libraries were bisulfite converted prior to capture with a pre-designed probe pool (Roche-NimbleGen, Madison, WI) to enrich the methylation analysis to 80.5Mb of the genome, covering ~2.7 x 106 CpG sites (intra- and intergenic CpG islands, and gene promoters were thoroughly covered by this method, while CpG shores, enhancers and gene bodies were not as thoroughly investigated).
ENA-FIRST-PUBLIC
2018-11-23T17:04:22Z
ENA-LAST-UPDATE
2018-06-29T15:28:58Z
External Id
SAMEA4772483
INSDC center name
UZH
INSDC first public
2018-11-23T17:04:22Z
INSDC last update
2018-06-29T15:28:58Z
INSDC status
public
Submitter Id
E-MTAB-6949:C6_normal
age
77
braf status
normal
broker name
ArrayExpress
colon segment involved
normal
common name
human
disease
colon cancer
disease staging
normal
individual
C6
kras status
normal
microsatellite status
normal
organism part
colon
sample name
E-MTAB-6949:C6_normal
sampling site
normal tissue adjacent to neoplasm
scientific_name
Homo sapiens
sex
female