Saccharomyces cerevisiae
Protocols: Grown in SCD media lacking uracil at 30C to exponential phase (OD600 ~0.6), pelleted by centrifugation and snap frozen into liquid nitrogen. Samples lysed in Buffer A using a 6870 Freezer Mill (Spex). Lysates cleared by centrifuging twice at 15000 g. Nucleic acids extracted using Trizol Reagent. rRNA was depleted from samples using Ribo-ZeroTM Magnetic Gold Kit for RNA-Seq. Depleted samples were precipitated with ethanol, and re-suspended in 10ul DEPC water. Sequencing libraries were then generated using the TruSeq Stranded mRNA sample preparation guide from ilumina from the Incubate RFP step, with 5ul of the ribosome depleted RNA added to 13ul of the fragment prime and finish mix.
ENA-FIRST-PUBLIC
2015-09-30T17:24:33Z
ENA-LAST-UPDATE
2018-03-08T20:14:32Z
External Id
SAMEA2747218
INSDC center name
The University of Manchester
INSDC first public
2015-09-30T17:24:33Z
INSDC last update
2018-03-08T20:14:32Z
INSDC status
public
Submitter Id
E-MTAB-2892:LC8
broker name
ArrayExpress
common name
baker's yeast
genetic modification
targeted mutation
genotype
his3delta1; leu2delta0; met15delta0; ura3delta0; caf20::G418; pCAF20m2-FLAG::URA3
sample name
E-MTAB-2892:LC8
scientific_name
Saccharomyces cerevisiae
sex
Mat a
specimen with known storage state
fresh specimen
strain
BY4741