Saccharomyces cerevisiae
B-carotene pathway inserted into chromosome 15 of S. cerevisiae CEN.PK113-7D. Two plasmids were also inserted; a high-expression plasmid (2u) for gRNA expression and a low-expression plasmid (ARSH4/CEN6) with dCas9.
strain
FEY_2
isolate
CEN.PK113-7D ChrXV::(Psbtdh3-crtE-Trpl41b//Psptdh3-crtYB-Tyol036w//Phta2-hyg-Tagtef1//Psmtef1-crtI-Trpl15a) pAG700::(Ptdh3-dCas9-Tadh1//Pagtef1-nat-Tagtef1//CEN6/ARSH4//AmpR//ColE1) pAG_22-2::(Pscsnr52-22_2-Tsup4//Pagtef1-kan-Tagtef1//2u//CmR//ColE1)
isolation_source
Engineered isolate
collection_date
2016-03
geo_loc_name
USA: Cambridge, Voigt Lab
sample_type
cell culture
BioSampleModel
Microbe, viral or environmental