Mus musculus
Protocols: Animals were sacrificed by cervical dislocation. The respiratory tract of mice was prepared under the sterile bench, washed in sterile phosphate buffered saline (PBS) and sectioned into trachea, bronchi and lungs. Tissues were separately transferred into fresh tubes, shock frozen in liquid nitrogen and stored at -80 °C until further use. 20-40 mg of lungs, ≤ 20 mg bronchi and ≤ 10 mg of trachea tissues were used for each RNA extraction. Homogenization of lung tissue was performed with pre-cooled mortar/ pestle and liquid nitrogen. The corresponding lysate was further homogenized using syringe and needle (0.8 mm). Homogenization of bronchi and trachea tissues were performed with Lysing Matrix H (MP Biomedicals) and a Retsch MM200 homogenizer at 30 Hz for 2.5 min, three times with 1 min incubation on ice in between. Total RNA isolation from homogenized tissues was performed using the RNeasy Kit (QIAGEN) applying the DNase digestion step according to the manufacturer's protocol. Mice were bred and housed in the animal facility of the Philipps-University Marburg under specific pathogen free conditions. For isolation of total RNA from murine airway tissues 8 to 16 weeks old wild type mice (C57BL/6 background) were used. Total RNA was isolated using RNA-Easy-Minikit followed by cDNA synthesis with random hexamer primers and RevertAid H Minus First Strand cDNA Synthesis Kit (ThermoFisher). RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina).
ENA-FIRST-PUBLIC
2020-03-27T17:05:05Z
ENA-LAST-UPDATE
2019-12-09T15:19:15Z
External Id
SAMEA6377065
INSDC center name
Institute of Molecular Oncology, Center for Tumor and Immunobiology, Philipps-University Marburg
INSDC first public
2020-03-27T17:05:05Z
INSDC last update
2019-12-09T15:19:15Z
INSDC status
public
Submitter Id
E-MTAB-8573:Trachea_3
age
8 to 16
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
wild type genotype
organism part
trachea
sample name
E-MTAB-8573:Trachea_3
scientific_name
Mus musculus
sex
male
strain
C57BL/6J