Drosophila melanogaster
Bosch et al. 2020 figure
Sup. 1D
Biological replicate
1
FACS enrichment for GFP+
No
PE2 source
pAct-PE2
pegRNA/sgRNA source
pCFD3-PE-ebony[23bpBC]
Condition
NA
Amplicon
ebony
Replicate
rep1
Sample type
Transfected S2R+ cells
Amplicon target
ebony
strain
S2R+/PT5 cells
age
not applicable
sex
not applicable
tissue
S2R+/PT5 cells
biomaterial_provider
Justin Bosch
genotype
pAct-PE2 and pegRNA plasmid transfection
Temperature raised
25˚C
pegRNA spacer
CTGGCCATCTGGAAGGCTGG
pegRNA extension
GAAGCTGGGATCGATGGGCAAATACGCGCCGCCAAACAGGCCGCAAGTGTTCTTAGGGCCTTCCAGATGG
nicking spacer
N/A
Forward primer
GAGGGATTGGTCACCACACT
Reverse primer
GGGATTTGGCATACAGTTCG
WT amplicon
GAGGGATTGGTCACCACACTGCTGGCCATCTGGAAGGCTGGCGGCGCGTATTTGCCCATCGATCCCAGCTTCCCGGCGAACCGCATTCACCACATACTGCTGGAGGCGAAGCCCACCTTGGTGATTCGCGACGATGACATCGACGCCGGCCGTTTCCAGGGAACTCCCACGTTATCCACCACCGAACTGTATGCCAAATCCC
Edited amplicon
GAGGGATTGGTCACCACACTGCTGGCCATCTGGAAGGCcctaagaacacttgcggcctgttTGGCGGCGCGTATTTGCCCATCGATCCCAGCTTCCCGGCGAACCGCATTCACCACATACTGCTGGAGGCGAAGCCCACCTTGGTGATTCGCGACGATGACATCGACGCCGGCCGTTTCCAGGGAACTCCCACGTTATCCACCACCGAACTGTATGCCAAATCCC
BioSampleModel
Model organism or animal