Drosophila melanogaster
Bosch et al. 2020 figure
Fig. 2E
Biological replicate
1
FACS enrichment for GFP+
not applicable
PE2 source
Act>PE2
pegRNA/sgRNA source
attP40
Condition
25˚C
Amplicon
white
Replicate
rep1
Sample type
Transgenic flies
Amplicon target
white
strain
w1118
dev_stage
adult
sex
male
tissue
whole fly
biomaterial_provider
Justin Bosch
genotype
Act>PE2 x attP40
Temperature raised
25˚C
pegRNA spacer
N/A
pegRNA extension
N/A
nicking spacer
N/A
Forward primer
TTCGCAGTCGGCTGATCTGT
Reverse primer
CACAGGTTGGCCATTGAGCA
WT amplicon
TTCGCAGTCGGCTGATCTGTGTGAAATCTTAATAAAGGGTCCAATTACCAATTTGAAACTCAGTTTGCGGCGTGGCCTATCCGGGCGAACTTTTGGCCGTGATGGGCAGTTCCGGTGCCGGAAAGACGACCCTGCTGAATGCCCTTGCCTTTCGATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCGACTGCTCAATGGCCAACCTGTG
Edited amplicon
TTCGCAGTCGGCTGATCTGTGTGAAATCTTAATAAAGGGTCCAATTACCAATTTGAAACTCAGTTTGCGGCGTGGCCTATCCGGGCGAACTTTTGGCCGTGATGGGCAGTTCCGGTTAAAGAAAGACGACCCTGCTGAATGCCCTTGCCTTTCGATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCGACTGCTCAATGGCCAACCTGTG
BioSampleModel
Model organism or animal