Zaire ebolavirus
As genomic sequencing can greatly facilitate public health efforts to identify and characterize the infectious agent as well as to contain and monitor an epidemic, our research team was deployed to undertake real-time sequencing using the Oxford Nanopore Technologies MinION™ sequencer in support of the local diagnostic teams during the May 2018 Ebola virus outbreak in the Equateur province of Democratic Republic of the Congo (DRC). As a pilot study, we investigated a confirmed Ebola-positive blood sample from the May 2017 Likati outbreak at the Institut National de Recherche Biomédicale (INRB), Kinshasa. The sample, collected on 7 May 2017, originated from a 22-year-old male (onset of disease 30 April 2017, deceased 8 May 2017). Here, we present the complete genome sequence of this Ebola virus isolate, obtained using MinION™ sequencer. We were able to generate results in less than 24 hours, with the sequencing workflow started from the viral RNA extraction to raw MinION data generation.
ENA-FIRST-PUBLIC
2019-04-10T04:03:03Z
ENA-LAST-UPDATE
2019-04-09T18:12:37Z
External Id
SAMEA5561116
INSDC center name
KU LEUVEN - UNIVERSITY OF LEUVEN
INSDC first public
2019-04-10T04:03:03Z
INSDC last update
2019-04-09T18:12:37Z
INSDC status
public
Submitter Id
1
collecting institution
Institut National de Recherche Biomédicale
collection date
2017-05-07
collector name
INRB
geographic location (country and/or sea)
Democratic Republic of the Congo
geographic location (latitude)
3.35 N
geographic location (longitude)
23.88 E
geographic location (region and locality)
Likati health zone in the province of Bas Uele
host age
22
host common name
Human
host disease outcome
dead
host health state
not provided
host scientific name
Homo sapiens
host sex
male
host subject id
EVD sample 12
isolate
Zaire ebolavirus
sample capture status
active surveillance in response to outbreak
sample name
1
scientific_name
Zaire ebolavirus
virus identifier
Zaire ebolavirus