Mus musculus
Protocols: Ngn3^EGFP mice [B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mice] were intercrossed. Embryos were isolated at E15.5. EGFP-positive embryos were separated into Ngn3^EGFP/EGFP and Ngn3^EGFP/+ pools (these could be easily recognized due to the EGFP level differences in the two genotypes: Ngn3^EGFP/EGFP homozygous mice have low EGFP in the pancreas, but high expression in the dudenum and nerve tissues; Ngn3^EGFP+ mice have high EGFP expression in the pancreas, but not in the duodenum. Most embryos were verified by PCR-based genotyping). The presence of vaginal plug at noon was considered as E0.5. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. Cell purity > 98%.
ENA-FIRST-PUBLIC
2015-10-01T17:02:40Z
ENA-LAST-UPDATE
2018-03-08T23:26:32Z
External Id
SAMEA3276650
INSDC center name
Vanderbilt University
INSDC first public
2015-10-01T17:02:40Z
INSDC last update
2018-03-08T23:26:32Z
INSDC status
public
Submitter Id
E-MTAB-3352:GG59 pool of 15 embryos (8 litters)
age
E15.5
broker name
ArrayExpress
common name
house mouse
sample name
E-MTAB-3352:GG59 pool of 15 embryos (8 litters)
scientific_name
Mus musculus
strain
B6.129S-Neurog3tm1(EGFP)Khk/Mmcd