marine metagenome
This sample (TARA_R110000782) was collected at station TARA_194 during campaign TARA_20130907Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130911T2000Z_194_EVENT_PUMP) occurred at position latitudeN=73.3833 and longitudeE=-168.1333, on date/time=2013-09-11T20:00:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (<0.22 micrometres) and prepared on board using protocol [SEQ-(FeCl3)_W<0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of viruses. This sample has no known replicate.
Chlorophyll Sensor
0.1417
Citation
Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)
ENA first public
2018-08-29
ENA last update
2016-08-25
Event Date/Time End
2013-09-11T23:00:00
External Id
SAMEA4397859
INSDC center alias
GSC
INSDC center name
Genoscope
INSDC first public
2018-08-29T17:02:51Z
INSDC last update
2016-08-25T12:12:57Z
INSDC status
public
Latitude End
73.3833
Longitude End
-168.3333
Salinity Sensor
27.38155
Submitter Id
TARA_R110000782
depth
5
environment (biome)
Polar Biome
environment (feature)
[SRF] surface water layer (ENVO:00010504)
environment (feature) further details
discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)
environment (material)
water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)
environmental package
water
event date/time start
2013-09-11T20:00:00
event label
TARA_20130911T2000Z_194_EVENT_PUMP
further details
http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20130912Z_194_WETLAB_VIRUS.pdf
last update date
2016-04-23Z
latitude start
73.3833
longitude start
-168.1333
marine region
[AO] Arctic Ocean (MRGID:1906)
nitrate sensor
-3.147755
oxygen sensor
369.339
project name
Tara Oceans Expedition (2009-2013)
protocol description
In the size fraction W<0.22 micrometres, the sample material was pre-filtered using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane (ref:GPWP14250) with 0.22-micrometres-pore-size. A volume of 20 Litres was treated with FeCl3, incubated at room temperature for 1-24 hours to allow viruses to precipitate, filtered onto a 1-micrometre-pore-size, 142-mm-diameter GE polycarbonate (PC) membrane filter (ref:K10CP14220) placed on top of a 0.8-micrometre-pore-size, 142-mm-diameter Pall Gelman support filter (ref:60114) using a peristaltic pump with pressure <15 psi. The 1-micrometre-pore-size filter was then packaged in a sterile Falcon tube (50 mL) (up to 3 filters in 1 tube), labelled with a barcode identification sticker and stored on board in a refrigerator at +4 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The three replicates were incubated together. For 20 Litres of filtrate, 2 millilitres of FeCl3 stock solution (4.83 grams of FeCl3-6H2O + 100 millilitres of MilliQ water, stored at 4°C) was added (final concentration of 1 mg Fe per Litre of filtrate), and shaken vigorously for 1 minute. Note 4: Further details about the method are published here doi:10.1111/j.1758-2229.2010.00208.x. In 2009-2012, a total of 3 replicates were prepared and sent for later analysis to the University of Arizona (UA), Tucson, USA. In 2013, a total of 2 replicates were prepared, one sent to for later analysis to the University of Arizona (UA), Tucson, USA, and the other sent for later analysis to the Observatoire Océanologique de Banyuls sur Mer (OOB), France.
protocol label
SEQ-(FeCl3)_W<0.22
sample collection device
high volume peristaltic pump [HVP-PUMP]
sample material label
TARA_194_SRF_<-0.22
sample name
TARA_R110000782
sample status
This version can be used to provide data discovery services
sampling campaign
TARA_20130907Z
sampling platform
SV Tara
sampling station
TARA_194
size fraction lower threshold
<
size fraction upper threshold
0.22
temperature
-0.618833