Homo sapiens
Protocols: HEK 293 or HeLa cells were seeded and transfected with the indicated constructs for indicated time points. Sequences for substrates #1-9. Index ACAGTG GCA AAT GGC ATT CTG ACA TCC for -SAMHD1 Exp1, CcAAAG GCA AAT GGC ATT CTG ACA TCC for -SAMHD1 Exp2, GACGAC GCA AAT GGC ATT CTG ACA TCC for -SAMHD1 Exp3, TAATCG GCA AAT GGC ATT CTG ACA TCC for +SAMHD1 Exp1, ACTGAT GCA AAT GGC ATT CTG ACA TCC for +SAMHD1 Exp2, CAACTA GCA AAT GGC ATT CTG ACA TCC for +SAMHD1 Exp3. Sequences for substrates #11-18. Index GCA AAT GGC ATT CTG ACA TCC for +SAMHD1 Exp1, GCA AAT GGC ATT CTG ACA TCC for +SAMHD1 Exp2, GCA AAT GGC ATT CTG ACA TCC for -SAMHD1 Exp3, GCA AAT GGC ATT CTG ACA TCC for -SAMHD1 Exp4. Genomic DNA was isolated using the DNeasy Blood & Tissue Kit (QIAGEN) according to the manufacturers instructions. Repaired plasmids and genomic junctions were PCR-amplified using specific primers with different 6bp 5' tags. For genomic amplicons, the Nextera XT Index Kit (Illumina) was used. Amplicons were pooled and sequenced on the Illumina MiSeq platform.
ENA first public
2021-01-20
ENA last update
2020-12-09
External Id
SAMEA7699090
INSDC center alias
1 Department of Internal Medicine III with Haematology , Medical Oncology, Haemostaseology, Infectiology and Rheumatology, Oncologic Center, Salzburg Cancer Research Institute Laboratory for Immunological and Molecular Cancer Research (SCRI LIMCR), Paracelsus Medical University, Salzburg, Austria, Cancer Cluster Salzburg, Austria 2 Department of Biosciences, Paris Lodron University of Salzburg, Austria
INSDC center name
1 Department of Internal Medicine III with Haematology , Medical Oncology, Haemostaseology, Infectiology and Rheumatology, Oncologic Center, Salzburg Cancer Research Institute Laboratory for Immunological and Molecular Cancer Research (SCRI LIMCR), Paracelsus Medical University, Salzburg, Austria, Cancer Cluster Salzburg, Austria 2 Department of Biosciences, Paris Lodron University of Salzburg, Austria
INSDC first public
2021-01-20T04:11:37Z
INSDC last update
2020-12-09T12:17:57Z
INSDC status
public
Submitter Id
E-MTAB-83821607516265:NAIIB_N
broker name
ArrayExpress
cell line
HEK293
common name
human
developmental stage
embryo
disease
normal
genetic modification
4 nCas9 constructs
genotype
SAMHD1 -/-
organism part
kidney
sample name
E-MTAB-83821607516265:NAIIB_N