Mus musculus
Protocols: For end point sacrificing and sample collection, the mice were not fasted for longer than 2-3 hours due to the hypoglycemia associated with the mutant phenotype. All samples were collected at the same time of the day during the light period of the mice. The mice were anesthetized with pentobarbital, blood was collected into Li-heparin tubes by cardiac puncture, and plasma was separated. Organs were immediately dissected and snap frozen in liquid nitrogen or immersed in 10% buffered formalin solution. Approximately 1 mm3 pieces of tissue were fixed in 1.5% glutaraldehyde, 1.5% paraformaldehyde in 0.1 M Sörensen buffer pH 7.2 for electron microscopy. Standard RNA isolation protocol for total RNA was used For RNA sequencing, rRNA was removed and the sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat.
ENA-FIRST-PUBLIC
2018-11-16T17:03:33Z
ENA-LAST-UPDATE
2018-11-15T17:40:01Z
External Id
SAMEA5090679
INSDC center name
DNA Sequencing and Genomics Lab, Institute of Biotechnology, HiLIFE, University of Helsinki
INSDC first public
2018-11-16T17:03:33Z
INSDC last update
2018-11-15T17:40:01Z
INSDC status
public
Submitter Id
E-MTAB-7416:Sample 57
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
disease
normal
genotype
Tg(Cint\AOX)
individual
1228
organism part
heart
sample name
E-MTAB-7416:Sample 57
scientific_name
Mus musculus
sex
female
strain
C57BL/6JCrl