Medicago sativa
Seeds of Medicago sativa (cv. Zhaodong) were germinated, and transferred on a mix of perlite and sand . All seedlings were grown in growth chamber , and chamber temperature was between 18 C (night) and 24 C (day), the humidity ranged from 60% to 80%, and the light period was 14h. The seedlings were irrigated with half-strength Hoagland solution once every two days, and after eight weeks they were randomly divided into three groups for stress treatments. For control group (untreated, A group), the seedlings were continuing to grow at condition as previously described. For cold stress (B group) and freezing stress (C group), the seedlings were transferred into another chamber with temperature set at 4 C or -8 C respectively. All seedlings were harvested at 3 h after stress treatments, five whole seedlings from each group were bulked separately. And all samples were frozen in liquid nitrogen, and stored at -80 C until use.
cultivar
Zhaodong
age
eight weeks
geo_loc_name
China:Harbin
tissue
whole seedings
BioSampleModel
Plant