Arabidopsis thaliana
Cytokinins have long been known to delay senescence in detached leaves. Here, we performed RNA-seq analysis to evaluate possible mechanisms of cytokinin-delayed senescence. Cotyledons were excised from 12 day-old Arabidopsis thaliana seedlings and were floated on MES buffer pH 5.7. Buffer was supplemented with either 1uM cytokinin or an equivalent volume (0.1%) DMSO as a vehicle control. Cytokinins tested included iP, iP-7-glucoside, and iP-9-glucoside. The treatment took place over six days in the dark to allow senescence to take place. At the end of the six days, RNA was extracted.
ecotype
Columbia
age
18d
sex
not applicable
tissue
Cotyledon
BioSampleModel
Model organism or animal