Drosophila melanogaster
Bosch et al. 2020 figure
Fig. 2E
Biological replicate
3
FACS enrichment for GFP+
not applicable
PE2 source
Act>PE2
pegRNA/sgRNA source
pCFD5-PE3-forked[D111X]
Condition
29˚C
Amplicon
forked
Replicate
rep3
Sample type
Transgenic flies
Amplicon target
forked
strain
w1118
dev_stage
adult
sex
male
tissue
whole fly
biomaterial_provider
Justin Bosch
genotype
Act>PE2 x pCFD5-PE3-forked[D111X]
Temperature raised
29˚C
pegRNA spacer
TTGTACGTCCGTGCACGCGA
pegRNA extension
ATCGGTGCCATTCtttaGCGTGCACGGACGT
nicking spacer
ATCTACTCACCATCCATTTG
Forward primer
ACGATGTCACGCCCGTTTAC
Reverse primer
CAACTGCTGCAGTTGGCCAA
WT amplicon
ACGATGTCACGCCCGTTTACCTGGCGGCGCAGGAGGGCCACTTGGAGGTGCTCAAGTTTCTGGTGCTCGAGGCCGGCGGCTCTTTGTACGTCCGTGCACGCGACGGAATGGCACCGATCCATGCCGCTTCACAAATGGGCTGCTTGGATTGCCTCAAATGGATGGTGAGTAGATGGTGCACCAAATGCATCGAATTCGGTCTTTTAATCCGATCTGTAAGCAGCTAACTGCCAACTTGGCTGGCCATCTTGGCCAACTGCAGCAGTTG
Edited amplicon
ACGATGTCACGCCCGTTTACCTGGCGGCGCAGGAGGGCCACTTGGAGGTGCTCAAGTTTCTGGTGCTCGAGGCCGGCGGCTCTTTGTACGTCCGTGCACGCTAAAGAATGGCACCGATCCATGCCGCTTCACAAATGGGCTGCTTGGATTGCCTCAAATGGATGGTGAGTAGATGGTGCACCAAATGCATCGAATTCGGTCTTTTAATCCGATCTGTAAGCAGCTAACTGCCAACTTGGCTGGCCATCTTGGCCAACTGCAGCAGTTG
BioSampleModel
Model organism or animal