Mus musculus
Protocols: Single-cell suspensions were prepared from spleens isolated from naive (n=3 per group) or from infected (n=4 per group) Trail+/+ or Trail-/- mice, 1 day post infection with LCMV. NK cells (defined as NK1.1+ and CD3‐ cells) were sort-purified by flow cytometry and resuspended in TRI-reagent. RNA was isolated according to the manufacturer's instructions and RNA concentration and integrity were assessed using a Bioanalyzer 2100. Barcoded stranded mRNA sequencing libraries were prepared from high quality total RNA samples (~10 ng/sample) using combination of the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490, Ipswich, MA, USA) to enrich the samples for polyadenylated RNA transcripts and the Ultra II Directional RNA Library Prep Kit (NEB #E7760).
ENA-FIRST-PUBLIC
2019-08-27T04:04:02Z
ENA-LAST-UPDATE
2018-12-18T15:38:12Z
External Id
SAMEA5187664
INSDC center name
University of Bern, Institute of Pathology
INSDC first public
2019-08-27T04:04:02Z
INSDC last update
2018-12-18T15:38:12Z
INSDC status
public
Submitter Id
E-MTAB-7562:Sample 10
age
8 to 12
broker name
ArrayExpress
cell type
natural killer cell
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
mixed pool of 4 mice
infect
Lymphocytic choriomeningitis mammarenavirus
organism part
spleen
phenotype
NK1.1 positive; CD3 negative
sample name
E-MTAB-7562:Sample 10
scientific_name
Mus musculus
sex
mixed
strain
C57BL/6J