Mus musculus
Protocols: Total RNA isolation from MLE-15 cells and primary murine AECII was performed using the RNeasy Kit (QIAGEN) applying the DNase digestion step according to the manufacturer's protocol. All cell growth and incubations were carried out at 37°C and 5% CO2. Human embryonic kidney 293 (HEK293) cells, Madin-Darby canine kidney cell lines MDCK(H) and MDCK(II) and MLE-15 mouse lung epithelial cells were maintained in Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal calf serum (FCS), 5% glutamine and 5% penicillin/streptomycin (=DMEM/FCS). Preparation of primary AECII was conducted according to the protocol previously described (Limburg et al., 2019) Total RNA was isolated using RNA-Easy-Minikit followed by cDNA synthesis with random hexamer primers and RevertAid H Minus First Strand cDNA Synthesis Kit (ThermoFisher). RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina).
ENA-FIRST-PUBLIC
2020-03-27T17:05:05Z
ENA-LAST-UPDATE
2019-12-09T14:54:40Z
External Id
SAMEA6377040
INSDC center name
Institute of Molecular Oncology, Center for Tumor and Immunobiology, Philipps-University Marburg
INSDC first public
2020-03-27T17:05:05Z
INSDC last update
2019-12-09T14:54:40Z
INSDC status
public
Submitter Id
E-MTAB-8571:Leukocytes_2
broker name
ArrayExpress
cell type
leukocyte
common name
house mouse
developmental stage
adult
disease
influenza
genotype
wild type genotype
organism part
lymph
sample name
E-MTAB-8571:Leukocytes_2
scientific_name
Mus musculus