Drosophila melanogaster
Bosch et al. 2020 figure
Fig. 2E
Biological replicate
2
FACS enrichment for GFP+
not applicable
PE2 source
tub>PE2
pegRNA/sgRNA source
pCFD3-PE-ebony[G111X]
Condition
29˚C
Amplicon
ebony
Replicate
rep2
Sample type
Transgenic flies
Amplicon target
ebony
strain
w1118
dev_stage
adult
sex
male
tissue
whole fly
biomaterial_provider
Justin Bosch
genotype
tub>PE2 x pCFD3-PE-ebony[G111X]
Temperature raised
29˚C
pegRNA spacer
CTGGCCATCTGGAAGGCTGG
pegRNA extension
GGCAAATACGCGCtttaAGCCTTCCAGATGG
nicking spacer
N/A
Forward primer
CCGGTTCCTGCAGCCAAACA
Reverse primer
GGGATTTGGCATACAGTTCG
WT amplicon
CCGGTTCCTGCAGCCAAACAGCGATGGTGACTTCATCGTGGCTGTGTGCATGCAGCCGTCGGAGGGATTGGTCACCACACTGCTGGCCATCTGGAAGGCTGGCGGCGCGTATTTGCCCATCGATCCCAGCTTCCCGGCGAACCGCATTCACCACATACTGCTGGAGGCGAAGCCCACCTTGGTGATTCGCGACGATGACATCGACGCCGGCCGTTTCCAGGGAACTCCCACGTTATCCACCACCGAACTGTATGCCAAATCCC
Edited amplicon
CCGGTTCCTGCAGCCAAACAGCGATGGTGACTTCATCGTGGCTGTGTGCATGCAGCCGTCGGAGGGATTGGTCACCACACTGCTGGCCATCTGGAAGGCTTAAAGCGCGTATTTGCCCATCGATCCCAGCTTCCCGGCGAACCGCATTCACCACATACTGCTGGAGGCGAAGCCCACCTTGGTGATTCGCGACGATGACATCGACGCCGGCCGTTTCCAGGGAACTCCCACGTTATCCACCACCGAACTGTATGCCAAATCCC
BioSampleModel
Model organism or animal