Homo sapiens
DDX4 FACS sorted single cell. FACS was performed on pooled ovarian cortical tissue from two individuals, 33 and 36 years old. Protocols: Single cells (n = 41) were randomly picked by mouth pipetting with a finely pulled glass capillary and placed directly into lysis buffer containing 1 uL of RNase inhibitor and 19 uL of 0.2% (vol/vol) Triton X-100 solution (Sigma-Aldrich, Germany). The 8-strip PCR tubes containing cells were immediately frozen on dry ice and kept at -80C for downstream applications. Regarding spike-ins: 0.1uL of a 4*10^-6 dilution of the ERCC RNA spike-in mix 1 (Ambion) was added to each sample. Picelli et al, Full-length RNA-seq from single cells using Smart-seq2, Nature Protocols, 2014
ENA-FIRST-PUBLIC
2015-10-01T17:02:38Z
ENA-LAST-UPDATE
2018-03-08T20:57:12Z
External Id
SAMEA2797542
INSDC center name
Department of Cell and Molecular Biology, Karolinska Institutet
INSDC first public
2015-10-01T17:02:38Z
INSDC last update
2018-03-08T20:57:12Z
INSDC status
public
Submitter Id
E-MTAB-2983:P36
broker name
ArrayExpress
common name
human
developmental stage
adult
individual
34
organism part
animal ovary
sample name
E-MTAB-2983:P36
scientific_name
Homo sapiens
sex
female
single cell well quality
OK