Mus musculus
Protocols: Splenic B cells were collected from TG2-sufficient and TG2-deficient mouse under resting and stimulated (2 µg / ml murine TG2 for 120 min) conditions. Total RNA was isolated from B cells with an RNeasy minikit (Qiagen). RNA integrity was analyzed on Agilent Bioanalyzer using Agilent RNA 6000 Pico kit. RNA integrity was 9.1 ± 0.7. RNA-seq libraries were generated from purified rRNA-depleted RNA samples using the strand-specific TruSeq stranded mRNA library prep kit (Illumina, San Diego, CA, USA).
ENA-FIRST-PUBLIC
2019-11-08T04:04:44Z
ENA-LAST-UPDATE
2019-11-07T12:37:46Z
External Id
SAMEA6162962
INSDC center name
K. G Jebsen centre for Coeliac disease research, Department of Immunology, Institute of Clinical Medicine, University of Oslo
INSDC first public
2019-11-08T04:04:44Z
INSDC last update
2019-11-07T12:37:46Z
INSDC status
public
Submitter Id
E-MTAB-7567:Sample 5
age
11
broker name
ArrayExpress
cell type
B cell
common name
house mouse
genotype
14E06 Ig KI HLA-DQ2.5 tg TG2 KO
individual
576
organism part
spleen
phenotype
Tgm2-
sample name
E-MTAB-7567:Sample 5
scientific_name
Mus musculus
sex
male
strain
C57BL/6