metagenome
ENA first public
2017-11-29
ENA last update
2017-11-20
External Id
SAMEA104393865
INSDC center alias
UNIVERSITY OF BRITISH COLUMBIA
INSDC center name
UNIVERSITY OF BRITISH COLUMBIA
INSDC first public
2017-11-29T17:03:26Z
INSDC last update
2017-11-20T21:10:53Z
INSDC status
public
Submitter Id
water.Loneincube.Mast.2
adapters
AATGATACGGCGACCACCGAGATCTACACGCT, CAAGCAGAAGACGGCATACGAGAT
collection date
2016-09-13
environment (biome)
aquarium
environment (feature)
saline water aquarium
environment (material)
water
geographic location (country and/or sea)
Canada
geographic location (depth)
0
geographic location (latitude)
49.265308
geographic location (longitude)
-123.249217
investigation type
mimarks-survey
multiplex identifiers
GGAGGAGCAATA
nucleic acid extraction
http://www.earthmicrobiome.org/protocols-and-standards/dna-extraction-protocol/
pcr conditions
initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 94
pcr primers
FWD:GTGYCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT
project name
seaweed_microbe_horiz_transmission
sample collection device or method
bottle
sample material processing
filter
sample name
water.Loneincube.Mast.2
sample size
500
sequencing method
Illumina MiSeq
specific host
environmental
target gene
16s rRNA
target subfragment
V4
water environmental package
water