Oikopleura dioica
Total RNA extracted and isolated with RNeasy Micro Kit (Qiagen, 74004) from O. dioica fully matured adults (4 days after hatching). The O. dioica individuals were collected from the on-doing laboratory culture deriving from the wild population in Ishikawa Harbor, Okinawa. Further processing for mRNA selection was performed with Oligo-d(T)25 Magnetic Beads (NEB, E7490). Adapters for the creation of DNA libraries for the Illumina platform were added per manufacturer's guidance (NEB, E7805) as were unique indexed oligonucleotides (NEB, E7600) to each of the 3 staged samples. The cDNA library was sequenced paired-end with a 300-cycle MiSeq Reagent Kit v2 (Illumina, MS-102-2002) loaded at approximately 12 pM. The RNA-Seq data was used to assemble transcriptome of the Okinawan O. dioica with Trinity software and to predict gene model in the I69-5 assembly.
ENA-FIRST-PUBLIC
2020-09-08T04:07:23Z
ENA-LAST-UPDATE
2020-09-02T08:19:21Z
External Id
SAMEA7282650
INSDC center name
OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY
INSDC first public
2020-09-08T04:07:23Z
INSDC last update
2020-09-02T08:19:21Z
INSDC status
public
Submitter Id
5
collected by
Andrew W. Liu, Yongkai Tan, Okinawa Institute of Science and Technology Graduate University, Genomics and Regulatory Systems Unit
dev stage
fully matured adults (4 days after hatching)
isolation source
on-going laboratory culture maintained at OIST
sample name
5
scientific_name
Oikopleura dioica
sex
mixed males and females