Mus musculus
Protocols: Thymi from 3-6 weeks old mice were enzymaticaly digested, cells were MACS enriched for CD45- fraction and FACS sorted using BD Influx sorter. mTECshigh were gated as EpCAM+CD11c-Ly51-MHCII+CD80+ and sorted directly to the RNA lysis buffer. Total RNA from FACS-sorted cells was extracted using an RNeasy Plus Micro Kit (Qiagen) and reverse transcribed using RevertAid (ThermoFisher) transcriptase and random hexamers (ThermoFisher). Libraries were prepared with a SMARTer Stranded Total RNA-Seq Pico Input Mammalian library preparation kit v2 (Takara).
ENA-FIRST-PUBLIC
2020-03-20T17:05:11Z
ENA-LAST-UPDATE
2019-06-10T11:34:55Z
External Id
SAMEA5702932
INSDC center name
Service Laboratory of Functional Genomics and Bioinformatics Institute of Molecular Genetics of the ASCR, v. v. i.
INSDC first public
2020-03-20T17:05:11Z
INSDC last update
2019-06-10T11:34:55Z
INSDC status
public
Submitter Id
E-MTAB-8024:WT5
age
3 to 6
broker name
ArrayExpress
cell type
Thymic antigen presenting cells, mTECs
common name
house mouse
genotype
MyD88fl/fl
individual
7
organism part
thymus
sample name
E-MTAB-8024:WT5
scientific_name
Mus musculus
sex
male
strain
C57BL/6J