Homo sapiens
Protocols: Tissue was dissociated in ice-cold PBS buffer and then subjected to UV-C irradiation. Cells were then precipitated by centrifugation for 1 min at 14000 rpm and snap-frozen on dry ice. The iCLIP protocol was followed to isolate RNA bound to FUS protein and amplify a cDNA library as described in the manuscript. The NB100-565 antibody (Novus Biologicals, US) was used at a concentration of 5 ug/mg on human brains. Specific barcodes were used for each RT reaction, with random nucleotides also incorporated into cDNAs to distinguish between PCR duplication of cDNA products; 20090826_LUd6_15: TGNNN, 20100222_LUd12_9: TGNNN, 20100222_LUd12_10: TCNNN. The final products were obtained by PCR with Illumina paired-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
ENA-FIRST-PUBLIC
2015-05-05T17:02:12Z
ENA-LAST-UPDATE
2018-03-09T00:05:54Z
External Id
SAMEA3373804
INSDC center name
Department of Molecular Neuroscience, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UK. MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
INSDC first public
2015-05-05T17:02:12Z
INSDC last update
2018-03-09T00:05:54Z
INSDC status
public
Submitter Id
E-MTAB-3534:20100222_LUd12_9
broker name
ArrayExpress
common name
human
immunoprecipitate
NB100-565 antibody
individual
3
organism part
brain
sample name
E-MTAB-3534:20100222_LUd12_9
scientific_name
Homo sapiens