activated sludge metagenome
ENA-FIRST-PUBLIC
2018-11-20T17:04:04Z
ENA-LAST-UPDATE
2018-11-20T11:08:33Z
External Id
SAMEA5096298
INSDC center name
AAU-BIO
INSDC first public
2018-11-20T17:04:04Z
INSDC last update
2018-11-20T11:08:33Z
INSDC status
public
Submitter Id
core_AAW_2012_10_901
chimera check
ChimeraSlayer
collection date
2012-10-16
common name
activated sludge metagenome
environment (biome)
freshwater biome [ENVO:00000873]
environment (feature)
wastewater treatment plant [ENVO:00002043]
environment (material)
activated sludge [ENVO:00002046]
geographic location (country and/or sea)
Denmark
geographic location (latitude)
0
geographic location (longitude)
0
investigation type
mimarks-survey
nucleic acid extraction
http://www.mpbio.com/US/Pages/Product.aspx?pid=116560200
pcr conditions
initial denaturation: 95degC_5min; annealing: 50degC_0.5min; elongation: 72degC_1min; final elongation: 72degC_7min; total cycles: 20. Adaptor annealling: denaturation: 95degC_5min; annealing: 50degC_0.5min; elongation: 72degC_1min; final elongation: 72degC_7min; total cycles: 7
pcr primers
FWD:GTGCCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT
project name
DK-sludge-core
reactor type
enhanced biological phosphorus removal
sample name
core_AAW_2012_10_901
scientific_name
activated sludge metagenome
sequencing method
Illumina
target gene
16S rRNA
target subfragment
V4
wastewater/sludge environmental package
wastewater/sludge