Mus musculus
Protocols: Brown adipose tissue was collected from young C57Bl6 mice (10 weeks old) and old C57Bl6 mice (76 weeks old) treated with or without β3 adrenergic receptor agonist (CL-316, 243) at 1 mg kg-1 under thermoneutrality for 5 days. Young C57Bl6 mice (10 weeks old) and old C57Bl6 mice (76 weeks old) were acclimatized under thermoneutrality at 30 degrees for 7 days and treated with β3 adrenergic receptor agonist (CL-316, 243) or saline control at 1 mg kg-1 for 5 days. Total RNA was extracted from brown adipose tissue of young C57Bl6 mice (10 weeks old) and old C57Bl6 mice (76 weeks old) treated with or without β3 adrenergic receptor agonist (CL-316, 243) using the RNeasy Mini Kit (Qiagen).RNA quality (RIN: RNA Integrity Number) was determined by Bioanalyzer (Agilent Technologies) and samples with RIN of >8.0 were subjected to RNA-seq. RNA Sequencing libraries were constructed from total RNA using Universal Plus mRNA-Seq (NuGEN).
ENA-FIRST-PUBLIC
2019-07-20T04:03:43Z
ENA-LAST-UPDATE
2018-11-28T21:25:12Z
External Id
SAMEA5139472
INSDC center name
UCSF
INSDC first public
2019-07-20T04:03:43Z
INSDC last update
2018-11-28T21:25:12Z
INSDC status
public
Submitter Id
E-MTAB-7445:Sample 10
age
76
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
mouse 10
organism part
brown adipose tissue
sample name
E-MTAB-7445:Sample 10
scientific_name
Mus musculus
sex
male
stimulus
beta(3)-adrenergic agonist Cl316,243
strain
C57BL/6