Mus musculus
Protocols: Postnatal pancreata were partially dissociated. Islets were hand picked and further dissociated into single cells for FACS-based beta cell purification. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacture's instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).
ENA-FIRST-PUBLIC
2015-10-01T17:02:36Z
ENA-LAST-UPDATE
2018-03-08T17:09:20Z
External Id
SAMEA2342550
INSDC center name
University of Pennsylvania
INSDC first public
2015-10-01T17:02:36Z
INSDC last update
2018-03-08T17:09:20Z
INSDC status
public
Submitter Id
E-MTAB-2266:P12 MIP-GFP mice 2
age
postnatal day 12
broker name
ArrayExpress
cell type
insulin-expressing mature beta cell
common name
house mouse
developmental stage
juvenile stage
sample name
E-MTAB-2266:P12 MIP-GFP mice 2
scientific_name
Mus musculus
strain
B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain