Drosophila melanogaster
isolate
Relaxed selection S2-F2-75 of SAIP from DGRP
age
11-14 days
dev_stage
adult
sex
female
tissue
whole body
biomaterial_provider
Yazmin L. Serrano Negron
birth_location
National Heart Lung and Blood Institute
breeding_history
We created the Sleep Advanced Intercross Population (SAIP) from the 5 longest-sleeping and 5 shortest-sleeping lines of the Drosophila Genetic Reference Panel (DGRP). The lines we used were DGRP-235, DGRP-313, DGRP-335, DGRP-338, and DGRP-379 (long sleepers); and DGRP-38, DGRP-310, DGRP-365, DGRP-808, and DGRP-832 (short sleepers). These lines are maximally homozygous; the genome-wide heterozygosity is less than 5%. DGRP-38 is heterozygous for the In(3R)K inversion; DGRP-313 is homozygous for the In(2L)t inversion; DGRP-335 is heterozygous for the In(3R)Mo inversion; DGRP-338 is heterozygous for the In(2L)t and In(2R)NS inversions; and the remaining lines have the standard karyotype for all common polymorphic inversions. DGRP-310, DGRP-335, DGRP-338, DGRP-365, and DGRP-832 were infected with Wolbachia pipientis; the remaining lines were not. All ten lines were crossed in a full diallel design, resulting in 100 crosses. Two virgin females and two males from the F1 of each cross were randomly mixed together and placed into 20 bottles, with 10 males and 10 females in each bottle. At each generation thereafter, 20 virgin females and 20 males from each bottle were randomly mixed with the other flies, and a new generation of 20 bottles was reared. Each generation of random mating had a census population size of 800. The genomes of these flies were allowed to recombine in this manner for 21 generations, resulting in an outbred population of flies.
breeding_method
We began with the SAIP base population derived from 5 longest-sleeping and 5 shortest-sleeping lines of the DGRP. We measured sleep in 100 virgin females and 100 virgin males, and chose the 25 individuals of each sex with the shortest night sleep duration to establish short sleeper selection populations. For 13 generations, we chose the 25 shortest-sleeping virgin males and the 25 shortest-sleeping virgin females from 100 measured of each sex as parents of the next generation. We relaxed selection by mating 25 males and 25 females at random for 62 additional generations.
collected_by
Laboratory of Systems Genetics
geo_loc_name
USA: Bethesda, MD
phenotype
Sleep duration
sample_type
whole organism
treatment
Relaxed selection
BioSampleModel
Model organism or animal