Saccharomyces cerevisiae
Protocols: The dead volume was collected with a syringe and discarded. For transcriptome sampling, biomass was collected from the reactor with a syringe and injected into chilled 50 ml Falcon tubes filled with 35 mL crushed ice. Samples were centrifuged for 4 min at 3,000 x g at 4oC; cell pellets were washed once with 1 mL of chilled water, transferred into Eppendorf tubes, flash frozen in liquid nitrogen, and stored at -80oC until analysis. The yeast Saccharomyces cerevisiae CEN.PK113-7D (MATa, MAL2-8c, SUC2) was used for all experiments. Cells were stored in aliquoted glycerol stocks at -80oC. Chemostat experiments were carried out under carbon or nitrogen-limited conditions on minimal mineral medium at a constant specific growth rate of 0.2 h-1, at 30oC, pH 5, working volume 0.5 L, aeration 1 vvm, pO2 >30%, agitation 800 rpm. Chemostat experiments were carried out in DASGIP 1L bioreactors (Jülich, Germany) equipped with off-gas analysis, pH, temperature and dissolved oxygen sensors. Chemostat medium contained glucose and (NH4)2SO4 as indicated in Fig S1a, as well as: KH2PO4, 3 g/L; MgSO4·7H2O, 0.5 g/L; trace metals solution, 1 ml/L; vitamin solution, 1 ml/L; antifoam, 0.1 ml/L. The trace metals solution contained: EDTA (sodium salt), 15.0 g/L; ZnSO4⋅7H2O, 4.5 g/L; MnCl2⋅2H2O, 0.84 g/L; CoCl2⋅6H2O, 0.3 g/L; CuSO4⋅5H2O, 0.3 g/L; Na2MoO4⋅2H2O, 0.4 g/L; CaCl2⋅2H2O, 4.5 g/L; FeSO4⋅7H2O, 3.0 g/L; H3BO3, 1.0 g/L; and KI, 0.10 g/L. The vitamin solution contained: biotin, 0.05 g/L; p-amino benzoic acid, 0.2 g/L; nicotinic acid, 1 g/L; Ca-pantothenate, 1 g/L; pyridoxine-HCl, 1 g/L; thiamine-HCl, 1 g/L and myo-inositol, 25 g/L. RNA was extracted using Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany). The Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA) was used to prepare mRNA samples for sequencing.
ENA-FIRST-PUBLIC
2020-03-12T17:04:23Z
ENA-LAST-UPDATE
2019-08-14T14:43:32Z
External Id
SAMEA5864392
INSDC center name
Division of Systems and Synthetic Biology Department of Biology and Biological Engineering Chalmers University of Technology
INSDC first public
2020-03-12T17:04:23Z
INSDC last update
2019-08-14T14:43:32Z
INSDC status
public
Submitter Id
E-MTAB-8245:N50.1
age
steady-state
broker name
ArrayExpress
common name
baker's yeast
growth condition
C/N=50
replicate
1
sample name
E-MTAB-8245:N50.1
scientific_name
Saccharomyces cerevisiae