soil metagenome
Factors influencing production of greenhouse gases nitrous oxide (N2O) and nitrogen (N2) in arable soils include high nitrate and moisture; we investigate how differences in the soil microbiome due to antecedent soil treatment and plants influence denitrification. Microbial communities, denitrification gene abundance and gas production in soils from tilled arable plots with contrasting fertilizer inputs (no N, mineral N, FYM) and regenerated woodland in the long-term Broadbalk field experiment were investigated. Soil was transferred to pots, kept bare or planted with wheat and after 6 weeks, transferred to sealed chambers with or without K15NO3 fertilizer for 4 days; N2O and N2 were measured daily. Concentrations of N2O were higher when fertilizer was added, lower in the presence of plants, whilst N2 increased over time and with plants. Prior soil treatment but not exposure to N-fertiliser or plants during the experiment influenced denitrification gene (nirK, nirS, nosZI, nosZII) relative abundance. Under our experimental conditions, denitrification generated mostly N2; N2O was around 2% of total gaseous N. Prior long-term soil management influenced the soil microbiome and abundance of denitrification genes. The production of N2O was driven by nitrate availability and N2 generation increased in the presence of plants.
ENA-FIRST-PUBLIC
2020-09-08T17:06:06Z
ENA-LAST-UPDATE
2020-05-15T15:43:40Z
External Id
SAMEA6838835
INSDC center name
ROTHAMSTED RESEARCH
INSDC first public
2020-09-08T17:06:06Z
INSDC last update
2020-05-15T15:43:40Z
INSDC status
public
Submitter Id
BB14_W_2
amount or size of sample collected
150
collection date
2014-04-20
composite design/sieving (if any)
Homogeneous subsample removed for DNA extraction sieved at 2mm
current vegetation
Deciduous woodland
environment (biome)
Temperate climate
environment (feature)
Broadbalk arable field under various fertilizer treatments and broadbalk deciduous woodland
environment (material)
Bulk soil
geographic location (country and/or sea)
United Kingdom
geographic location (depth)
0-0.2
geographic location (elevation)
129
geographic location (latitude)
51°48′35″
geographic location (longitude)
00°22′30
geographic location (region and locality)
Hertfordshire, Harpenden, Rothamsted Research
history/agrochemical additions
Woodland (Broadbalk Wilderness) receives no fertilizer treatments
history/previous land use
Broadbalk wilderness: Deciduous woodland since1883
investigation type
metagenome
miscellaneous parameter
total nitrogen 0.48%
multiplex identifiers
BB14.W.2: TTAGACTCGGAA
pcr primers
515f Forward Primer GTGYCAGCMGCCGCGGTAA, 806rB Reverse Primer GGACTACHVGGGTWTCTAAT
ph
7.7
ph method
pH measured in water: 1:2.5 soil: solution
project name
Broadbalk Long Term Classical Experiment Nitrogen Cycling 2014
sample collection device or method
10 x random soil cores (20 cm depth x 3 cm diameter) per replicate (~1.5kg).3 replicates per treatment
sample material processing
10 soil cores pooled, sieved (4mm) and mixed for pot experiments. Homogeneous subsample removed for DNA extraction
sample name
BB14_W_2
sample volume or weight for DNA extraction
2
sample weight for DNA extraction
2
scientific_name
soil metagenome
sequencing method
Illumina MiSeq Pyrosequencing
soil environmental package
soil
soil taxonomic/fao classification
Chromic Luvisol
soil taxonomic/local classification
Clay loam to silty clay loam over clay with flints
storage conditions (fresh/frozen/other)
Sieved soil for DNA extraction was frozen at -80 oC until processing.
target gene
16S rRNA gene
texture
15/52/29
total organic carbon
62.3