Herbaspirillum seropedicae SmR1
Protocols: H. seropedicae SmR1, streptomycin resistant, was grown at 30 degrees C and 120 rpm in NFbHPN medium (Klassen et al., 1997) in the presence (+Nar) or absence (-Nar) of 100 uM of naringenin containing streptomycin (80 ug.mL-1)for 6 hours (optical density at 600 nm of 0.8). Cells were collected by centrifugation. The total RNA was isolated using RiboPureTM-Bacteria Kit (Ambion) After rRNA depletion 500 ng of depleted rRNA was used to construct the sequencing libraries following standard protocols using the SOLiD Total RNA-Seq Kit (Life Technologies). The libraries were barcoded by using the SOLiD Transcriptome Multiplexing Kit (Life Technologies).
ENA first public
2015-04-30
ENA last update
2015-03-26
External Id
SAMEA3312971
INSDC center alias
UFPR
INSDC center name
UFPR
INSDC first public
2015-04-30T17:21:29Z
INSDC last update
2015-03-26T10:00:35Z
INSDC status
public
Submitter Id
E-MTAB-3435:-Nar2
broker name
ArrayExpress
sample name
E-MTAB-3435:-Nar2