Onchocerca ochengi
Nodules containing adult O. ochengi parasites were obtained from the skins of cattle slaughtered at Ngaoundéré abattoir in the Adamawa Region of Cameroon by Vincent Tanya and colleagues. Adult male and female worms were separated from excised nodules; the gonads were removed from the latter (selecting only nongravid individuals) and stored in PBS or RNAlater (Sigma) at −80°C, whereas the males were preserved whole. The material was transported to the United Kingdom on dry ice and stored at −80°C. Microfilaria were collected from excised nodules. For transcriptomic analyses, total RNA was purified from the worm material stored in RNAlater using TRI Reagent (Sigma). As the nematodes in the host are closely associated with host tissues, and eat host cells, the samples contain both O. ochengi and B. indicus RNA.
ENA-FIRST-PUBLIC
2013-05-22T17:00:44Z
ENA-LAST-UPDATE
2018-08-23T10:45:51Z
External Id
SAMEA2178390
INSDC center name
BANG
INSDC first public
2013-05-22T17:00:44Z
INSDC last update
2018-08-23T10:45:51Z
INSDC status
public
project id
2011172
sample name
Onchocerca_ochengi_rnaseq_sample31
scientific_name
Onchocerca ochengi