Homo sapiens
Protocols: Blood-derived lymphoblastoid cell lines for 36 individuals with autism spectrum disorder were obtained from the Simons Simplex Collection. For two individuals the age of diagnosis is missing due to lack of consent for distributing this information. Cells were cultured in Culture Medium (RPMI 1640, 2mM L-glutamine, 15% fetal bovine serum) to a maximum cell density of 1 × 106 cells/ml. When cells reached maximum density, they were passaged by dissociating the cells by pipetting up and down 4 or 5 times and seeding to a density of 200,000-500,000 viable cells/ml. Cells were grown under 37°C, 5% CO2 conditions for 10 days. Approximately 5 × 105 cells were then detached and spun down at 300 × g for 5 minutes at 4°C. RNA was extracted using RNeasy® Plus Micro Kit (QIAGEN) following manufacturer's protocol. RNA quality was assessed using Agilent RNA 6000 Nano Kit (Agilent Technologies) and ran on Bioanalyzer 2100 (Agilent Technologies). RNA-seq libraries were generated by TruSeq® Stranded Total RNA Library Prep Kit with Ribo-Zero Gold Set A (Illumina).
ENA-FIRST-PUBLIC
2018-11-25T17:03:25Z
ENA-LAST-UPDATE
2018-11-15T19:20:36Z
External Id
SAMEA5092014
INSDC center name
Department of Psychiatry, UCSF Weill Institute for Neurosciences
INSDC first public
2018-11-25T17:03:25Z
INSDC last update
2018-11-15T19:20:36Z
INSDC status
public
Submitter Id
E-MTAB-7351:Sample 5
age
12.7
broker name
ArrayExpress
cell type
lymphoblast
common name
human
developmental stage
juvenile stage
disease
autism spectrum disorder
individual
11414
organism part
blood
sample name
E-MTAB-7351:Sample 5
scientific_name
Homo sapiens
sex
female