Homo sapiens
Protocols: The human iPSC lines F14, F18, MRS2-6 and MRS2-10 were derived in house from fibroblasts acquired by skin punch biopsy, using the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific, #A16517) in accordance with the manufacturer's instructions. Human pluripotent stem cells were maintained in mTeSR1 media on Matrigel-coated tissue culture plastic, and differentiated into pancreatic progenitors using the STEMdiff Pancreatic Progenitor kit (STEMCELL Technologies, 05120). Differentiation was carried out according to the manufacturer's instructions with the following modifications: (1) cells were initially seeded into 12 well plates (Corning, 353043) at a density of 10e6 cells/well, and (2) stage 1 was extended to last 3 days - rather than 2 - by repeating the final day's treatment. Samples were harvested for RNA isolation on differentiation day 8. All tissue culture was carried out in a 5% CO2 air environment at 37oC. RNA was isolated from samples harvested from differentiation cultures using an RNeasy mini kit (Qiagen, cat # 74104). All RNA samples had an RNA integrity number > 8. RNA-seq libraries were generated using the NEBNext Ultra RNA Library Prep Kit, (NEB, E7530L). Messenger RNA was enriched by hybridization to oligo-dT.
ENA-FIRST-PUBLIC
2020-07-03T04:03:27Z
ENA-LAST-UPDATE
2020-06-23T17:35:44Z
External Id
SAMEA7014008
INSDC center name
Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR)
INSDC first public
2020-07-03T04:03:27Z
INSDC last update
2020-06-23T17:35:44Z
INSDC status
public
Submitter Id
E-MTAB-9243:Sample 2
broker name
ArrayExpress
cell line
F14
cell type
pancreatic endoderm
common name
human
disease
normal
genotype
Carrier RFX6 c.1129C>T
individual
father
progenitor cell type
induced pluripotent stem cell
replicate
2
sample name
E-MTAB-9243:Sample 2
sampling time point
differentiation day 8
scientific_name
Homo sapiens