Mus musculus
Protocols: T helper type 2 cells were extracted from mouse spleens and activated with CD3/CD28. Each mouse replicate is a separate purification of T cells from a collection of mice. Mice were 8-12 weeks old and of mixed gender. Cells were spinoculated with retroviruses overexpressing a gene of interest on day 1. IL4 is 10ng/ml, and the cells were in these for the remainder of the time spent in culture. For the overexpression for chipseq and rnaseq you can refer to Addgene plasmids #117263-117267. Further information can be found in the preprint here: https://www.biorxiv.org/content/early/2017/12/28/196022 or E-MTAB-6285. Lrrc40dec is a truncated version of Lrrc40. On day 4, BFP-positive (infected) cells were FACS sorted and measured by ChIPmentation. The antibody is 3xFLAG, SIGMA clone F1804 DNA was extracted as part of the ChIPmentation protocol Libraries were made as part of the ChIPmentation protocol
ENA-FIRST-PUBLIC
2018-11-23T17:03:08Z
ENA-LAST-UPDATE
2018-09-20T10:14:02Z
External Id
SAMEA4925803
INSDC center name
Sanger Institute / Karolinska Institutet
INSDC first public
2018-11-23T17:03:08Z
INSDC last update
2018-09-20T10:14:02Z
INSDC status
public
Submitter Id
E-MTAB-7258:BHLHE40_OE
age
8 to 12
broker name
ArrayExpress
cell type
T-helper 2 cell
common name
house mouse
genotype
MSCV-Bhlhe40-3xFLAG-IRES-BFP
organism part
spleen
phenotype
Bhlhe40 overexpression
sample name
E-MTAB-7258:BHLHE40_OE
scientific_name
Mus musculus
sex
mixed
stimulus
CD3/CD28
strain
CBLA