soil metagenome
Grazing intensity 8.67 sheep/ha
collection_date
2014-08-16
depth
0-10cm
elev
1650 m
env_biome
not applicable
env_feature
Grassland
env_material
Soil
geo_loc_name
China:Gansu
lat_lon
37.12 N 106.82 E
annual_season_precpt
359.3
annual_season_temp
7.1
ph
8.62
ph_meth
Soil and water ratio is 2.5:1
pool_dna_extracts
Mixed three DNA extraction,about 3000ng
previous_land_use
Long-term grazing
previous_land_use_meth
Free grazing
samp_collect_device
Five 0¨C10 cm-thick soil samples were randomly collected from each quadrat (1 m ¡Á 1 m) and immediately mixed into a single soil sample.
samp_size
0.5g
samp_vol_we_dna_ext
0.05ml
sieving
stored in a ¨C20¡ãC freezer
store_cond
One week
tot_n_meth
Soil total nitrogen was measured using the NC analyzer dry combustion method (Sumigraph NC-900; Sumika Chemical Analysis Service, Tokyo, Japan).
tot_nitro
0.64
tot_org_c_meth
Water and inorganic carbon were removed by reacting the samples with phosphoric acid, after which soil organic carbon was measured using the NC analyzer dry combustion method (Sumigraph NC-900; Sumika Chemical Analysis Service, Tokyo, Japan).
tot_org_carb
4.34
water_content_soil
9.673024523
water_content_soil_meth
the mass was stabilized by drying at 105¡ãC for 24 h, the water content of the soil was calculated
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.soil