Badumna sp. SGP-2016
Nucleic acid sequences and peptide sequences from venom-gland preparations from the spider Badumna Sp.
ENA first public
2018-11-15
ENA last update
2016-11-16
External Id
SAMEA4552016
INSDC center alias
Institute for Molecular Biosciences
INSDC center name
Institute for Molecular Biosciences
INSDC first public
2018-11-15T17:02:55Z
INSDC last update
2016-11-16T06:24:07Z
INSDC status
public
Submitter Id
GFKSPG_BadumnaSp1
broker name
Bioinformatics Resources Australia - EMBL
collection date
2013
geographic location (country and/or sea)
Australia
geographic location (region and locality)
Regency Downs
lat lon
27.53896839 S 152.43280559 E
library reads sequenced
135,730
relationship to oxygen
aerobe
sample material processing
All spiders used for complementary DNA (cDNA) library construction were first anesthetized using CO2, frozen at -80°C for 10 min, and then dissected at 4°C. To access the venom gland, the chelicerae were first removed from the base of the structure (towards the carapace). Once separated, each chelicera was individually cut from the ventral side up to the base of the fang in order to approach the venom gland from underneath. The cheliceral muscle that surrounds the venom gland was detached to expose the isolated venom gland. Dissected venom glands were placed immediately in TRIzol® reagent (Invitrogen) to be processed.
sample name
GFKSPG_BadumnaSp1