Homo sapiens
Protocols: T24 cells were obtained from American Type Culture Collection. To generate the cisplatin resistant cells, T24 were treated with a gradual increment (4, 8, 16, 32, 64 ug/ml cisplatin) with a discontinuous period until cells recover. 10^6 cells T24R and T24 cells were harvested for RNA-seq. 5 ug RNA was extracted by Trizol and treated with DNase I following the manufacturer's instructions. RNA quality was assessed using a Total RNA Nano chip with a 2100 Bioanalyzer instrument (Agilent). RNA-seq libraries were prepared from T24 cells using NEBNext Ultra II RNA library Prep Kit for Illumina (NEB,E7770S). Library quality was assessed using a DNA1000 chip with a 2100 Bioanalyzer instrument (Agilent).
ENA-FIRST-PUBLIC
2019-11-11T04:04:44Z
ENA-LAST-UPDATE
2018-11-28T15:57:40Z
External Id
SAMEA5139351
INSDC center name
Department of urology, Taizhou people's hospital, Taizhou, Jiangsu, 225300, China.
INSDC first public
2019-11-11T04:04:44Z
INSDC last update
2018-11-28T15:57:40Z
INSDC status
public
Submitter Id
E-MTAB-7437:Sample 2
broker name
ArrayExpress
cell line
T24
cell type
epithelial cell
common name
human
disease
Transitional Cell Carcinoma
genotype
wild type genotype
organism part
urinary bladder
sample name
E-MTAB-7437:Sample 2
scientific_name
Homo sapiens