activated sludge metagenome
Biomass fed stable-isotope labeled medium with no triclosan (control). Libraries were from DNA fractions separated in a density-gradient.
CsCl_fraction_density
1.7304
ENA-FIRST-PUBLIC
2013-06-03
ENA-LAST-UPDATE
2013-01-21
External Id
SAMEA1903985
INSDC center alias
AAU-BIO
INSDC center name
Section for Biotechnology, Aalborg University
INSDC first public
2013-06-03T13:10:14Z
INSDC last update
2013-01-21T20:11:57Z
INSDC status
public
Submitter Id
Control_2_12
chimera check
Perseus-AmpliconNoise v.1.25
collection date
10-11-2011
environment (biome)
freshwater biome [ENVO:00000873]
environment (feature)
wastewater treatment plant [ENVO:00002043]
environment (material)
activated sludge [ENVO:00002046]
environmental package
wastewater/sludge
experimental factor
efo:EFO_0000369
geographic location (country and/or sea,region)
Denmark
geographic location (latitude)
57.02923
geographic location (longitude)
9.94711
investigation type
mimarks-survey
nucleic acid extraction
http://www.mpbio.com/US/Pages/Product.aspx?pid=116560200
oxygenation status of sample
aerobic
pcr conditions
"16S PCR initial denaturation:95_5; annealing:50_0.5; elongation: 72_1; final elongation:72_7; total cycles: 20 ""adaptor annealling"" denaturation:95_5; annealing:50_0.5; elongation: 72_1; final elongation:72_7; total cycles: 7"
pcr primers
FWD:GAGAGTTTGATCCTGGCTCAG REV:ATTACCGCGGCTGCTGG
project name
PYRO-SIP
reactor type
enhanced biological phosphorus removal
sample name
Control_2_12
sequencing method
pyrosequencing
target gene
16S rRNA
target subfragment
V1-V3
triclosan_conc
0 mg/L