Mus musculus
Protocols: Mouse kidney tissues were removed capsule, and cut into small pieces in cold PBS, and added RIPA buffer (0.5% SDS) on ice for 30 min, then centrifuged at 13000 rpm for 15 min. The supernatant was harvested and treated by sonication to break genomic DNA into 200-500 bp. 10% were stored as input and the rest of supernatant was quantified the equal DNA concentration, then incubated with 1 μg IP grade antibodies of H3K4me3 (Activemotif, USA) 4 °C overnight. Protein-A beads were incubated next day at 4 °C for target protein pull down. template DNA was repaired to 3'-dA overhang and added the ligated adapter. The DNA library was eliminated the unligated adapters and selected the appropriate size for sequence using an Illumina Hiseq 2000 platform.
ENA-FIRST-PUBLIC
2020-07-03T04:03:13Z
ENA-LAST-UPDATE
2019-07-09T17:48:32Z
External Id
SAMEA5765098
INSDC center name
Department of Critical Care Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, 250000, China.
INSDC first public
2020-07-03T04:03:13Z
INSDC last update
2019-07-09T17:48:32Z
INSDC status
public
Submitter Id
E-MTAB-8133:AKI_H3K4me3_2
age
6 to 8
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
1
organism part
renal glomerulus
sample name
E-MTAB-8133:AKI_H3K4me3_2
scientific_name
Mus musculus
sex
male
strain
C57BL/6