Drosophila melanogaster
Bosch et al. 2020 figure
Fig. 1F
Biological replicate
1
FACS enrichment for GFP+
No
PE2 source
NA
pegRNA/sgRNA source
NA
Condition
NA
Amplicon
forked
Replicate
rep1
Sample type
Transfected S2R+ cells
Amplicon target
forked
strain
S2R+/PT5 cells
age
not applicable
sex
not applicable
tissue
S2R+/PT5 cells
biomaterial_provider
Justin Bosch
genotype
not applicable
Temperature raised
25˚C
pegRNA spacer
N/A
pegRNA extension
N/A
nicking spacer
N/A
Forward primer
ACGATGTCACGCCCGTTTAC
Reverse primer
CAACTGCTGCAGTTGGCCAA
WT amplicon
ACGATGTCACGCCCGTTTACCTGGCGGCGCAGGAGGGCCACTTGGAGGTGCTCAAGTTTCTGGTGCTCGAGGCCGGCGGCTCTTTGTACGTCCGTGCACGCGACGGAATGGCACCGATCCATGCCGCTTCACAAATGGGCTGCTTGGATTGCCTCAAATGGATGGTGAGTAGATGGTGCACCAAATGCATCGAATTCGGTCTTTTAATCCGATCTGTAAGCAGCTAACTGCCAACTTGGCTGGCCATCTTGGCCAACTGCAGCAGTTG
Edited amplicon
ACGATGTCACGCCCGTTTACCTGGCGGCGCAGGAGGGCCACTTGGAGGTGCTCAAGTTTCTGGTGCTCGAGGCCGGCGGCTCTTTGTACGTCCGTGCACGCTAAAGAATGGCACCGATCCATGCCGCTTCACAAATGGGCTGCTTGGATTGCCTCAAATGGATGGTGAGTAGATGGTGCACCAAATGCATCGAATTCGGTCTTTTAATCCGATCTGTAAGCAGCTAACTGCCAACTTGGCTGGCCATCTTGGCCAACTGCAGCAGTTG
BioSampleModel
Model organism or animal