Homo sapiens
Vero cells (ATCC, CCL-81) were maintained in DMEM (Gibco) and primary astrocytes were maintained in DMEM/F12 (Gibco). CHIKV isolate BHI3745/H804709 (GenBank accession #KP164570.1). Viral stocks used here CHIKV (1.6 x 108 PFU/ml) were propagated by serial passages in Vero cells by routine methods using DMEM. Astrocyte infections were performed at MOI 1 (2 x 105 cells/well) for the viruses in 12 well plate during 1 h at 37 C and 5% CO2 in medium without FBS.
isolate
Chikungunya3
age
missing
biomaterial_provider
missing
sex
missing
tissue
neural system
cell_line
Astrocyte
sample_type
cell culture
treatment
Chikungunya infected
BioSampleModel
Human