Saccharomyces cerevisiae
A dCas9 expression set integrated into the HO locus of S. cerevisiae CEN.PK113-7D. The strain also includes a high-expression (2u) plasmid for gRNA expression.
strain
FEY_15
isolate
CEN.PK113-7D HO::(Pgal10-dCas9Mx1-Tspo1//Psptef1-nat-Ttip1) pY128:(Psnr52-gRNAscr-TtracrSUP4//Pagtef1-kan-Tagtef1//2micron//CmR//ColE1)
isolation_source
Engineered isolate
collection_date
2018-04
geo_loc_name
USA: Worcester, Young Lab
sample_type
cell culture
BioSampleModel
Microbe, viral or environmental