Homo sapiens
Protocols: Blood and synovial fluid samples were collected with written informed patient consent from PsA patients undergoing intra-articular aspiration at Oxford University Hospitals. The study was performed in accordance with protocols approved by the Oxford Research Ethics committee (Ethics reference number 06/Q1606/139). Mononuclear cells were immediately isolated by density centrifugation then stained by a panel of fluorescently conjugated antibodies in staining buffer (RNAse-free PBS, 2mM EDTA). The following antibodies were used: CD3-FITC (SK7), CD4-APC (RPA-T4), CD8a-PE (RPA-T8), CD45RA-BV421 (HI100) (all BioLegend), together with eFluor780 viability dye (eBioscience). Stained cells were sorted into memory-enriched (CD45RA negative) CD3+CD4+CD8- or CD3+CD8+CD4- fractions, which were then combined in a 1:1 ratio before loading onto the 10x Chromium Controller. A separate channel was used for the synovial and blood derived cells from each patient. Standard 10x protocols for the kit were used to load cells into droplets containing barcodes. Cells were captured and cDNA preparation performed according to the Single Cell 5′ Protocol recommended by 10x Genomics.
ENA-FIRST-PUBLIC
2020-09-07T17:07:40Z
ENA-LAST-UPDATE
2020-08-28T04:10:09Z
External Id
SAMEA7279838
INSDC center name
University of Oxford
INSDC first public
2020-09-07T17:07:40Z
INSDC last update
2020-08-28T04:10:09Z
INSDC status
public
Submitter Id
E-MTAB-9492:1505_SF
age
35
broker name
ArrayExpress
cell type
T cell
common name
human
developmental stage
adult
disease
psoriatic arthritis
individual
PsA1505
organism part
synovial fluid
sample name
E-MTAB-9492:1505_SF
scientific_name
Homo sapiens
sex
male