Homo sapiens
Protocols: Flp-In 293 T-REx (Thermo Fisher Scientific) WT and CASC3-KO cells were maintained at 37° C, 5% CO2 and 90% humidity in Dublecco's Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 9% fetal bovine serum (FBS) and Penicillin-Streptomycin (both Thermo Fisher Scientific). For siRNA transfection, cells were reverse transfected with 60 pmol siRNA targeting CASC3 (or Luc in the negative control) per 2 x 10^5 cells using 2.5 μl Lipofectamine RNAiMAX. Total RNA was extracted with NucleoSpin RNA Plus (Macherey-Nagel) according to the manufacturer's instructions. Ribosomal depletion and strand specific library preparation was carried out with TruSeq Stranded Total RNA (with Ribo-Zero Gold) according to the manufacturer's instructions.
ENA-FIRST-PUBLIC
2020-03-14T04:03:22Z
ENA-LAST-UPDATE
2019-10-24T11:01:00Z
External Id
SAMEA6116901
INSDC center name
Section of Bioinformatics and Systems Cardiology, Department of Internal Medicine III and Klaus Tschira Institute for Integrative Computational Cardiology DZHK (German Centre for Cardiovascular Research), Partner site Heidelberg/Mannheim
INSDC first public
2020-03-14T04:03:22Z
INSDC last update
2019-10-24T11:01:00Z
INSDC status
public
Submitter Id
E-MTAB-8461:106036
broker name
ArrayExpress
cell line
Flp-In-T-REx-293
common name
human
genotype
CASC3 knockout (H)
sample name
E-MTAB-8461:106036
scientific_name
Homo sapiens