Homo sapiens
Protocols: The hESCs H9 (WA-09)-NURR1-GFP reporter line was maintained using E8-essential medium (Fisher Scientific) without feeder on VTA-N (Fisher Scientific) and passaged every 4-5 days by EDTA. Midbrain dopamine (mDA) differentiation from hESC was done with an optimized protocol from previously published by our group. ChIP was performed and DNA was purified with a magnetic bead-based purification method according to the manufacturer's protocol (MAGnify Chromatin Immunoprecipitation System-Kit, LifeTechnologies). sheared DNA was used to generate ChIP-sequencing libraries by employing Ovation Ultralow System V2 Kit (NuGEN).
ENA-FIRST-PUBLIC
2019-07-21T04:02:15Z
ENA-LAST-UPDATE
2018-08-24T19:17:51Z
External Id
SAMEA4858689
INSDC center name
The Rockefeller University Laboratory of Molecular and Cellular Neuroscience
INSDC first public
2019-07-21T04:02:15Z
INSDC last update
2018-08-24T19:17:51Z
INSDC status
public
Submitter Id
E-MTAB-59651535134514:MR19
broker name
ArrayExpress
cell line
H9
cell type
dopaminergic neuron
common name
human
genotype
NURR1::GFP
progenitor cell type
embryonic stem cell
sample name
E-MTAB-59651535134514:MR19
scientific_name
Homo sapiens