Homo sapiens
Protocols: Human polyA+ RNAs were purchased from Clontech. The RNAs were isolated from tissue samples using a modified guanidium thiocynate extraction method followed by polyA+ RNA selection with two rounds of oligo(dT)-cellulose columns. 5' and 3' RACE reactions were performed in 12.5 ul volume independently with each polyA+ RNA sample using the SMART RACE amplification kit (Clontech) according to the manufacturer's instructions. 7.5ul of each of the 400 lncRNA targets were pooled respecting tissues and directionality of the RACE reaction (5' and 3' RACE) and purified with the QIAquick kit (Quiagene) as described for RT-PCR-seq experiments [2]. A set of 25 control reactions targeting full-length annotated transcripts were RACEd in parallel as positive controls. 1.5ul of these controls were pooled with the other reactions prior to library preparation. Libraries were prepared according to the Rapid Library Preparation Method Manual for GS FLX+ Series / XL+ Kit.
ENA-FIRST-PUBLIC
2013-05-21T11:50:03Z
ENA-LAST-UPDATE
2018-03-08T16:27:05Z
External Id
SAMEA1906765
INSDC center name
Wellcome Trust Sanger Institute
INSDC first public
2013-05-21T11:50:03Z
INSDC last update
2018-03-08T16:27:05Z
INSDC status
public
Submitter Id
E-MTAB-1665:polyA+_liver_6100188
broker name
ArrayExpress
common name
human
organism part
liver
sample name
E-MTAB-1665:polyA+_liver_6100188
scientific_name
Homo sapiens
sex
male
specimen with known storage state
frozen sample