Drosophila melanogaster
Bosch et al. 2020 figure
Fig. 1F
Biological replicate
1
FACS enrichment for GFP+
Yes
PE2 source
pAct>PE2
pegRNA/sgRNA source
pCFD3-PE-white[A134X]
Condition
Sort
Amplicon
white
Replicate
rep1
Sample type
Transfected S2R+ cells
Amplicon target
white
strain
S2R+/PT5 cells
age
not applicable
sex
not applicable
tissue
S2R+/PT5 cells
biomaterial_provider
Justin Bosch
genotype
pAct>PE2 and pegRNA plasmid transfection
Temperature raised
25˚C
pegRNA spacer
GTGATGGGCAGTTCCGGTGC
pegRNA extension
AGGGTCGTCTTTCtttaACCGGAACTGCCCA
nicking spacer
N/A
Forward primer
TTCGCAGTCGGCTGATCTGT
Reverse primer
CACAGGTTGGCCATTGAGCA
WT amplicon
TTCGCAGTCGGCTGATCTGTGTGAAATCTTAATAAAGGGTCCAATTACCAATTTGAAACTCAGTTTGCGGCGTGGCCTATCCGGGCGAACTTTTGGCCGTGATGGGCAGTTCCGGTGCCGGAAAGACGACCCTGCTGAATGCCCTTGCCTTTCGATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCGACTGCTCAATGGCCAACCTGTG
Edited amplicon
TTCGCAGTCGGCTGATCTGTGTGAAATCTTAATAAAGGGTCCAATTACCAATTTGAAACTCAGTTTGCGGCGTGGCCTATCCGGGCGAACTTTTGGCCGTGATGGGCAGTTCCGGTTAAAGAAAGACGACCCTGCTGAATGCCCTTGCCTTTCGATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCGACTGCTCAATGGCCAACCTGTG
BioSampleModel
Model organism or animal