Drosophila melanogaster
Bosch et al. 2020 figure
Fig. 2E
Biological replicate
3
FACS enrichment for GFP+
not applicable
PE2 source
tub>PE2
pegRNA/sgRNA source
pCFD5-PE3-white[A134X]
Condition
29˚C
Amplicon
white
Replicate
rep3
Sample type
Transgenic flies
Amplicon target
white
strain
w1118
dev_stage
adult
sex
male
tissue
whole fly
biomaterial_provider
Justin Bosch
genotype
tub>PE2 x pCFD5-PE3-white[A134X]
Temperature raised
29˚C
pegRNA spacer
GTGATGGGCAGTTCCGGTGC
pegRNA extension
AGGGTCGTCTTTCtttaACCGGAACTGCCCA
nicking spacer
TTGAGCAGTCGCATCCCGGA
Forward primer
TTCGCAGTCGGCTGATCTGT
Reverse primer
CACAGGTTGGCCATTGAGCA
WT amplicon
TTCGCAGTCGGCTGATCTGTGTGAAATCTTAATAAAGGGTCCAATTACCAATTTGAAACTCAGTTTGCGGCGTGGCCTATCCGGGCGAACTTTTGGCCGTGATGGGCAGTTCCGGTGCCGGAAAGACGACCCTGCTGAATGCCCTTGCCTTTCGATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCGACTGCTCAATGGCCAACCTGTG
Edited amplicon
TTCGCAGTCGGCTGATCTGTGTGAAATCTTAATAAAGGGTCCAATTACCAATTTGAAACTCAGTTTGCGGCGTGGCCTATCCGGGCGAACTTTTGGCCGTGATGGGCAGTTCCGGTTAAAGAAAGACGACCCTGCTGAATGCCCTTGCCTTTCGATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCGACTGCTCAATGGCCAACCTGTG
BioSampleModel
Model organism or animal