metagenome
ENA first public
2017-11-29
ENA last update
2017-11-20
External Id
SAMEA104393880
INSDC center alias
UNIVERSITY OF BRITISH COLUMBIA
INSDC center name
UNIVERSITY OF BRITISH COLUMBIA
INSDC first public
2017-11-29T17:03:26Z
INSDC last update
2017-11-20T21:10:54Z
INSDC status
public
Submitter Id
Nereocystis.Environmental.StarfishOuter2.1
adapters
AATGATACGGCGACCACCGAGATCTACACGCT, CAAGCAGAAGACGGCATACGAGAT
body site
blade
collection date
2016-08-01
environment (biome)
neritic epipelagic zone biome�
environment (feature)
rocky shore
environment (material)
alga
geographic location (country and/or sea)
Canada
geographic location (depth)
0
geographic location (latitude)
51.65425
geographic location (longitude)
-128.1213
host body product
mucus
investigation type
mimarks-survey
multiplex identifiers
ATCCATGAGCGT
nucleic acid extraction
http://www.earthmicrobiome.org/protocols-and-standards/dna-extraction-protocol/
pcr conditions
initial denaturation:98degC_0.017min; annealing:50degC_0.083min; elongation: 72degC_0.4min; final elongation:72degC_1min; 109
pcr primers
FWD:GTGYCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT
project name
seaweed_microbe_horiz_transmission
sample collection device or method
swab
sample material processing
snap off in cryotube
sample name
Nereocystis.Environmental.StarfishOuter2.1
sequencing method
Illumina MiSeq
specific host
Nereocystis leutkeana laboratory natural
target gene
16s rRNA
target subfragment
V4
water environmental package
water