Mus musculus
Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacture's instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).
ENA-FIRST-PUBLIC
2015-10-01T17:02:36Z
ENA-LAST-UPDATE
2018-03-08T17:09:10Z
External Id
SAMEA2342535
INSDC center name
University of Pennsylvania
INSDC first public
2015-10-01T17:02:36Z
INSDC last update
2018-03-08T17:09:10Z
INSDC status
public
Submitter Id
E-MTAB-2266:E15.5 Ngn3^GFP/GFP embryos 1
age
embryonic day 15.5
broker name
ArrayExpress
cell type
neurogenin-3-lacking pancreatic progenitor cell
common name
house mouse
developmental stage
embryo stage
sample name
E-MTAB-2266:E15.5 Ngn3^GFP/GFP embryos 1
scientific_name
Mus musculus
strain
B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain