Saccharomyces cerevisiae
Protocols: Grown in SCD media at 30C to exponential phase (OD600 0.5 - 0.7), pelleted by centrifugation and snap frozen into liquid nitrogen Samples lysed in Lola lysis buffer using a 6870 Freezer Mill (Spex). Lysates cleared by centrifugating twice at 15000 g. Nucleic acids extracted using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit. Depleted samples were precipitated with 2.5x volumes ethanol, 1/10th volume 3M sodium acetate and 1_l glycogen, washed twice with 70% ethanol and re-suspended in 10_l DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. cDNA was then amplified and barcoded with SOLiD RNA barcoding Kit and samples purified using PureLink PCR Micro Kit
ENA-FIRST-PUBLIC
2015-05-01T17:02:16Z
ENA-LAST-UPDATE
2018-03-08T17:36:23Z
External Id
SAMEA2521358
INSDC center name
The University of Manchester
INSDC first public
2015-05-01T17:02:16Z
INSDC last update
2018-03-08T17:36:23Z
INSDC status
public
Submitter Id
E-MTAB-2567:DSLF1-2
broker name
ArrayExpress
common name
baker's yeast
genetic modification
gene knock out
genotype
slf1-/-
sample name
E-MTAB-2567:DSLF1-2
scientific_name
Saccharomyces cerevisiae
sex
Mat a
specimen with known storage state
fresh specimen
strain
BY4741