aquatic metagenome
combination of culture dependent and culture independent methods of microbial characterization had been done on extremely polluted Adyar river . culture dependent method is by means of separating by growing in artificial media following by the colony morphology, gram staining and biochemical assay with the input of ABIS software.The culture independent method was done by amplification with V3 region of 16 s rRNA and sequenced with the NGS ILLUMINA HISEQ and process like trimming and OTU clustering was done by the software like MOTHUR and PyNAST and the rarefraction curve was made to show the species richness.
isolation_source
river
collection_date
2008-03-13
geo_loc_name
India:Chennai, Saidapet
lat_lon
13.0827 N 80.2707 E
samp_size
50 gram
sample_type
metagenomic assembly
ref_biomaterial
missing
samp_collect_device
manual
samp_mat_process
room temperature
source_material_id
saidapet metagenomics
BioSampleModel
Metagenome or environmental