phyllosphere metagenome
plant body site
adult vascular leaf PO:0006340
Investigation type
MIMARKS
Project name
gradient_pyrenees
Enviornmental package
host-associated
organism
phyllosphere metagenome
target gene
ITS1-ITS2
Environment (biome)
Temperate broadleaf and mixed forest biome ENVO:00000876
host taxid
28930
scientific_name
phyllosphere metagenome
Environment (material)
phyllosphere
ENA-KEYWORD
GSC, MIxS, MIMARKS
host common name
European beech
pcr primers
ITS2 : CGTATCGCCTCCCTCGCGCCATCAGGACTCGCTGCGTTCTTCATCGATGC ITS1F : CTATGCGCCTTGCCAGCCCGCTCAGCGCGACTTGGTCATTTAGAGGAAGTAA
Geographic location (longitude)
-0.07388889
Geographic location (latitude)
42.8925
pcr conditions
initial denaturation:95degC_5min; 30 cycles of denaturation:95degC_30sec; annealing=54degC_1min; extension=72degC_1min30sec; final extension=72degC_10min
Elevation
1533
ENA-FIRST-PUBLIC
2011-12-06T11:09:11Z
ENA-LAST-UPDATE
2018-03-09T09:55:08Z
Sequencing method
Pyrosequencing
Collection date
10th September 2009
Geographic location (country:region,area)
France
multiplex identifiers
GACTC
Environment (feature)
plant-associated habitat ENVO:00009001
ENA-FIRST-PUBLIC
2011-12-06
ENA-LAST-UPDATE
2018-03-09