Brassica napus
source_name
Cotyledons
brassica napus cultivar
Darmor-bzh
inoculation protocol
Ten days-old seedling of the susceptible cultivar Darmor-bzh were inoculated with 10µl of water at a needle puncture. Inoculated plants were incubated in darkness for 48h at room temperature under saturated humidity, and then transferred to a growth chamber at 16°C (night) and 24°C (day), with a 12h photoperiod. 7 days after infection, cotyledons were sampled.