Arabidopsis thaliana
Title
Ath_LEC_20in
Molecule
genomic_DNA
Extract_protocol
DNeasy Plant Mini Kit (Qiagen) was used to extract and purify total DNA from fresh leaf tissue collected on 3-week old plants. DNAs were quantified with a Picogreen kit by using a QPCR ABI7900. An equimolar pooling of separately extracted DNA was performed for each population, resulting in a final mean amount of DNA of 5μg
Growth_protocol
Seeds were collected from French A. thaliana populations  during March/April. Prior DNA extraction, seeds were sown in 7cm x 7 cm pots filled with a 1:1 mixture of sterile C2 and Metro 200 potting soils