Brassica napus
source_name
Stem
brassica napus cultivar
Eurol
inoculation protocol
Adult plants of the susceptible cultivar Eurol were inoculated 22 days after sowing at the three leaves development stage. The petiole of the second leaf was cut off at 1 cm from the leaf insertion and 10µl of water was placed over the wound.Inoculated plants were incubated in darkness for 48h at room temperature under saturated humidity, and then transferred to a growth chamber at 16°C (night) and 24°C (day), with a 12h photoperiod. 24 days after inoculation stem tissues were sampled.