Mus musculus
ArrayExpress-CellType
TS-Rs26
ArrayExpress-Species
Mus musculus
ENA first public
2017-01-24
ENA last update
2018-02-08
External Id
SAMEA4416001
INSDC center alias
SC
INSDC center name
Wellcome Sanger Institute
INSDC first public
2017-01-24T13:33:27Z
INSDC last update
2018-02-08T16:40:42Z
INSDC status
public
Submitter Id
193457c0-4d97-11e6-9a3d-3c4a9275d6c8
common name
house mouse
sample description
Total RNA from a single confirmed Crb2 knockout clone generated and grown in parallel to vector control clones was extracted using TRIzol reagent. Cells were plated at 20,000 cells/6-well well. The RNA sample is a pool of 2 6-well wells grown in parallel.Total RNA from a single vector control clone generated and grown in parallel to Crb2 HOMs clones was prepared as described above. Cells were plated at 20,000 cells/6-well well. The RNA sample is a pool of 2 6-well wells grown in parallel. Cells were plated in TSC conditions, media changed to Differentiation conditions the next day, and cells harvested 3 days later, i.e. 3D of differentiation. The Crb2 CRISPR knockout design deletes exons 7&8.
sample name
193457c0-4d97-11e6-9a3d-3c4a9275d6c8