Homo sapiens
isolate
Psoriatic patients who were diagnosed with psoriasis vulgaris were recruited from outpatient clinics in the Huashan Hospital of Fudan University. Psoriasis disease activity was assessed using PASI score. healthy controls were recruited from Ruijin Hospital of Shanghai Jiao Tong University School of Medicine. Sample acquisitions were approved by the Ethnics Committee of Ruijin Hospital and Renji Hospital of Shanghai Jiao Tong University School of Medicine and Huashan Hospital of Fudan University. All experiments were performed in accordance with the declaration of Helsinki Principles. Informed consent was obtained for all procedures.PBMCs were isolated with Ficoll.Total RNA was extracted using the miRNeasy Micro Kit and miRNA libraries were prepared by using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA) and then purified by QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and put to size selection by using gel Prep.
age
17
biomaterial_provider
Huashan Hospital and Renji Hospital, Shanghai,China
sex
male
tissue
PBMC2
BioSampleModel
Human