A genomic survey of Trebouxia sp. TR9, a microalga isolated from the lichen Ramalina farinacea
Trebouxia lynnae
Specimens of Ramalina farinacea (L.) Ach. were collected from seven different locations in the Iberian Peninsula and Canary Islands and from two sites in California (USA), as detailed in Table S1. Samples were dried out in the shaded open air for 1 day and then stored at -20*C until needed. For photobiont isolation, R. farinacea thalli were collected in the air-dried state on Quercus rotundifolia Lam. at Sierra El Toro (Castell*n, Spain). Samples were frozen at -20*C until the isolation experiment, 3 months after collection. TR1 and TR9 phycobionts were isolated in our laboratories according to Gasulla and colleagues (2010). Isolated phycobionts were cultured in liquid or semisolid Bold 3N medium (Bold and Parker, 1962) in a growth chamber at 15*C, under a 14 h/10 h light/dark cycle (light conditions: 25 mmol m-2 s-1).