Rhizosphere microbial communities were collected by saline washes of coarse roots (diameter 2-4 mm) of maize cultivar 3BT, collected at the flowering stage BBCH 63-65. Total DNA was extracted from the collected cells by “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France). The extraction included two bead beating steps of 45 s at 6.5 m s-1 on a FastPrep-24 system (MP Biomedicals) and additional washing steps of the binding matrix each with 1 ml 5.5 M guanidine thiocyanate (Carl Roth GmbH) until the matrix retained its original color (1 - 3 washes).
rhizosphere metagenome
Rhizosphere microbial communities were collected by saline washes of coarse roots (diameter 2-4 mm) of maize cultivar 3BT, collected at the flowering stage BBCH 63-65. Total DNA was extracted from the collected cells by “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France). The extraction included two bead beating steps of 45 s at 6.5 m s-1 on a FastPrep-24 system (MP Biomedicals) and additional washing steps of the binding matrix each with 1 ml 5.5 M guanidine thiocyanate (Carl Roth GmbH) until the matrix retained its original color (1 - 3 washes).