pyrosequencing of 16S rRNA gene amplicons of phylloepiphytic bacteria of packaged spinach (day15/4C)
metagenome
Fresh spinach was harvested and packaged to mimic minimally processing.  Spinach was held at 4C for 15 days. Nucleic acids from spinach phyllosphere were isolated and the V4 hypervariable region of the 16S rRNA gene was amplified using RDP primers and analyzed using RDP pyrosequencing pipeline.