Total lung RNA from WT mice
Mus musculus
Male 9-week-old wild type mice were sacrified and lungs were sampled for total RNA isolation. Total lung RNA was extracted with TRIzol reagent (Life technologies, USA). Sequencing sample libraries established from lungs of wild-type or WT mice were prepared using a NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs, NEB). Sequencing runs were performed on an Illumina Genome Analyzer IIx (Illumina).