Transcriptome analysis of the radish tuberous root using Illumina paired-end sequencing and development of EST-SSR markers
Raphanus sativus var. sativus
Samples composed of hypocotyl (1 cm) and true root (1 cm) tissues, which from 10 seedlings were collected at 7 days (RESS) after sowing. For Illumina sequencing, total RNA was extracted using TRIzol reagent (Invitrogen) and treated with RNase-free DNase I (TaKaRa, Japan) for 45 min in accordance with the manufacturer's protocol.