AID-/- in vitro activated mouse B cells used for Circularized Chromosome Conformation Capture (4C)
Mus musculus
CD43- resting B cells were isolated from AID-/- mouse spleens by negative selection with magnetic beads (Miltenyi Biotech). Following isolation, resting B cells were stimulated with LPS (50 mcg/ml) and IL-4 (2.5 ng/ml) for 72 hrs.