Sample 13
Homo sapiens
Protocols: HEK293T cells stably expressing our reporter construct were transfected with plasmids to induce gene editing to restore fluorescence of our reporter. Cells that turned fluorescence positive were separated using FACS and were subjected to MiSeq analysis FACS HEK293T cells were lysed and gDNA was extracted using a Qiagen DNA extraction kit gDNA was amplified using gene specific primers targeted towards our reporter vector, the fancf gene, or the emx1 gene. Primary PCR products were gel purified using a Thermo DNA extraction kit and then amplified using Illumia MiSeq adapter primers.