Plant sample from Glycine max
Glycine max
Four lines with the following phenotypes were selected for this study from the chromosome segment substitution line (CSSL) library based on Suinong14 (SN14) and ZYD00006 (wild soybean): high protein high oil (HPHO), low protein low oil (LPLO), low protein high oil (LPHO), and high protein low oil (HPLO). Samples were taken from three representative periods of seed size standard (EM1,MM, DS) during the development of the soybean seed as provided on the website (Grant, 2010). The interval between every two samplings was approximately 20 days from July to the end of September.