Mus musculus pre-B cell (Btk -/-)
Mus musculus
For CTCF ChIP-Seq in pre-B cells we used IL-7-driven BM cultures from Btk-deficient B cells that have increased pre-B cell proliferation and normal Ig locus accessibility (Hendriks and Middendorp, 2004) and a rabbit-anti-mouse-CTCF polyclonal antibody (#07-729; Millipore). ChIP procedures followed by ultrahigh-throughput DNA sequencing using the Illumina Genome Analyzer II platform, have been described previously (Soler et al. 2010). All sample information show in the following:  cell: pre-B cell (Btk -/-)  cell organism: mouse  cell description: primary pre-B cell from bone-marrow  cell sex: M  antibody: CTCF  antibody antibodydescription: rabbit polyclonal  antibody targetdescription: the insulator protein CTCF  antibody vendorname: Millipore  antibody vendorid: 07-729  treatment: IL-7  treatment description: expanded in culture in the presence of IL-7  control: IgG  control description: Rabbit IgG ChIP-seq reads  age: 6 weeks  age description: 6 weeks  control: IgG  control description: Rabbit IgG ChIP-seq reads  controlid: IgG  replicate: 1  replicate description: tier 1  strain: C57BL/6  strain description: C57BL/6  Biomaterial provider  Hendriks lab  Treatment protocol  None  Growth protocol  Ribeiro de Almeida et al. Immunity 2011  Extracted molecule  genomic DNA  Extraction protocol  Soler et al. Genes and Development 2010