Composition and function of a bacterial community shed light on the pest status of Rhizoglyphus robini
Rhizoglyphus robini
Laboratory-reared populations of Rhizoglyphus robini, originally collected from infested bulbs of three plant species: Alium sp., Lilium sp. and Ruscus sp.. the populations were maintained on ground peanuts as food source. from each population, pools of ~250 individuals were collected (three replicates for each population). DNA was extracted using the PowerSoil* DNA Isolation Kit (Mo Bio Laboratories, Inc., California, USA) according to the manufacturer's instructions. Tag-encoded sequencing of 16S rRNA gene fragments (V4-V6) was conducted at the Research and Testing Laboratory (Lubbock, Texas, USA). The different populations are denoted: R for Ruscus; G for Alium; L for Lilium. The population letter is followed by the replicate number (1-3).