Reads from Thaumetopoea pityocampa, EP (early population)
Thaumetopoea pityocampa
This BioSample describes Thaumetopoea pityocampa Reads from an italian population called EP (early population). These reads are used for the construction of a reference transcriptome (see bioproject). Eggs were collected in Cimolais-Italy. They were reared in the laboratory at room temperature (average 22.0C, interval 20- 25C) and natural light. RNA was extracted from various developmental stages : larvae (L1, L2, L3, L4 and L5), pupae and adult. RNA was extracted using a Trizol extraction procedure. After verification of RNA quality and integrity the final libraries were sequenced using an illumina Hiseq2000 using the paired-end protocole (2 by 100nt).