Invertebrate sample from Labriocimbex Yan & Wei, gen. nov.
Labriocimbex sinicus
Labriocimbex sinica Yan & Wei sp. nov., a large and hairy species of the Labriocimbex Yan & Wei, gen. nov., and the mitochondrial genome of Labriocimbex were firstly reported in this paper. The new genus of Cimbicidae was found to be closely similar to Pseudoclavellaria Schultz and Trichiosoma Leach. A key to genera of Trichiosomini was provided. In order to identify the systematic placement of Cimbicidae, the mitochondrial genome of L. sinica was assembled using high-throughput sequencing data. The complete mitochondrial genome of L. sinica was obtained with length of 15405 bp (GenBank: MH136623) and a typical set of 37 genes (22 tRNAs, 13 PCGs, two rRNAs). Results demonstrated that all protein-coding genes (PCGs) were initiated by ATN codons, and ended with TAA or T stop codons. Our study revealed that all transfer RNA genes (tRNAs) had a typical clover-leaf secondary structure, except trnS1. Remarkably, the secondary structure of L. sinica of rrnS and rrnL were very different from Corynis lateralis (C. lateralis). Phylogenetic analyses verified the monophyly and positions of three Cimbicidae species within the superfamily Tenthredinoidea and recovered a relationship of (Tenthredinidae + Cimbicidae) + (Argidae + Pergidae) in Symphyta with strong nodal supports. Furthermore, we found that phylogenetic tree based on two methods showed that L. sinica is sister group of Trichiosoma anthracinum (T. anthracinum) with high support values. To sum up, we suggest that Labriocimbex belongs to the tribe of Trichiosomini as well as Trichiosoma.