Rhizosphere microbial communities were collected by saline washes of fine roots (diameter > mm) of maize cultivar 3BT, collected at the flowering stage BBCH 63-65. Individual plants were sampled. Total DNA was extracted from the collected cells by “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France). The extraction included two bead beating steps of 45 s at 6.5 m s-1 on a FastPrep-24 system (MP Biomedicals) and additional washing steps of the binding matrix each with 1 ml 5.5 M guanidine thiocyanate (Carl Roth GmbH) until the matrix retained its original color (1 - 3 washes).
rhizosphere metagenome
Rhizosphere microbial communities were collected by saline washes of fine roots (diameter > mm) of maize cultivar 3BT, collected at the flowering stage BBCH 63-65. Individual plants were sampled. Total DNA was extracted from the collected cells by “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France). The extraction included two bead beating steps of 45 s at 6.5 m s-1 on a FastPrep-24 system (MP Biomedicals) and additional washing steps of the binding matrix each with 1 ml 5.5 M guanidine thiocyanate (Carl Roth GmbH) until the matrix retained its original color (1 - 3 washes).