Sample 11
Drosophila melanogaster
Protocols: RNA was prepared from adult fly heads Fly stocks and crosses were maintained on standard media (1% agar, 1.5% sucrose, 3% glucose, 3.5% dried yeast, 1.5% maize, 1% wheat, 1% soya, 3% treacle, 0.5% propionic acid, 0.1% Nipagin) RNA was extracted from 20 heads per sample in five independent samples. Heads were homogenised in TRI Reagent (Sigma) by using a motorised pestle and following the manufacturer's instructions. The guidelines from the Illumina TruSeq Stranded mRNA Sample Preparation Guide (Part 15031047 Rev. E, October 2013) were followed