F2-2
Homo sapiens
Protocols: VCF and VNCF were isolated from fetal human cardiac fibroblast by MACS against VCAM1. Fetal human cardiac fibroblasts were cultured in HFDM-1(+) medium supplemented with 1% (v/v) Newborn Calf Serum (NBCS). After initial expansion, fibroblasts were incubated with a biotin-conjugated anti-CD106 (VCAM1) antibody and anti-biotin microbeads for isolation of VCF and VNCF by MACS. Total RNA was extracted using the RNeasy Plus Mini Kit following the manufacturer's instructions (Qiagen). The library was constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) by GENEWIZ.