Transcriptome analysis of the radish tuberous root using Illumina paired-end sequencing and development of EST-SSR markers
Raphanus sativus var. sativus
Samples composed of hypocotyl (1 cm) and true root (1 cm) tissues, which from 10 seedlings were collected at 20 days (RLSS, the stage of cortex splitting) after sowing. For Illumina sequencing, total RNA was extracted using TRIzol reagent (Invitrogen) and treated with RNase-free DNase I (TaKaRa, Japan) for 45 min in accordance with the manufacturer's protocol.