Metagenome or environmental sample from anaerobic digester metagenome
anaerobic digester metagenome
The EGSB (Expanded Granular Sludge Bed) reactor was installed in the Sewage Treatment Plant (STP) of Sao Carlos, Sao Paulo (Brazil) and operated with hydraulic retention time (HRT) of 36 h. Physical-chemical and chromatographic analyzes were performed for characterization of sanitary sewage and reactor monitoring. COD and LAS of 653.50 mg L-1 and 6,189 mg L-1, respectively, were observed in the sanitary sewage. Iron and potentially toxic metals have been observed, such as Cadmium, Lead, Manganese, Nickel and Zinc. Were also observed the presence of the recalcitrant compounds Butyl Benzenesulfonamide, Hexadecanoic Acid, Limonene, Terpinene, Phenol, Phenylethyl Alcohol, Indolizine, Caffeine and Isobutyl Octadecyl Phthalate. The reactor was operated for 314 days and COD removal efficiency of 75.5% and LAS removal efficiency of 60.37% were observed . By means of the mass balance it was verified that 56.2% of the LAS were removed, 12.8% adsorbed and 43.4% biodegraded. During the EGSB operation, the granules were disrupted. This fact corroborates with the higher effluent concentration of total solids and total volatile solids in the first month and at the end of the operation compared to the general average. 18 genres related to LAS degradation, such as Synergistes, Syntrophorhabdus, Syntrophus, Clostridium, Geobacter and Desulfovibrio were identified through the Illumina MiSeq platform.