Pupa mixed age 1, 2, 3, 4, 5 day RNA-Seq
Drosophila melanogaster
CS flies were reared at room temperature (23-24C) for collection.  Third-instar wandering larva were picked into a separate vial.  Separate collections of pupa were made at 1, 2, 3, and 5 days old.  Whole body samples were homogenized in 1ml of Trizol (Invitrogen, Carlsbad, California, USA) per 50-100 mg of tissue using a glass-Teflon homogenizer.  RNA was purified according to the suggested manufacturer's protocol (Invitrogen).  RNA was mixed in equimolar amounts and sequenced on the Illumina GA II instrument for 75bp paired-end reads.