Empc1 Co1 rep2
Mus musculus
Protocols: Cells were harvested by trypsinisation. Total RNA from cells were extracted using RNeasy Mini kit 74104 Qiagen, Manchester, UK), cDNA library preparation was carried out at Oxford Genomics Centre, The Wellcome Trust Centre for Human Genetics using PolyA+ RNA enrichment method for total RNA from cultured cells . mRNA fraction was selected from the total RNA before conversion to cDNA. Second strand cDNA synthesis incorporated dUTP. The cDNA was end-repaired, A-tailed and adapter-ligated. Prior to amplification, samples underwent uridine digestion. The prepared libraries were size selected, multiplexed and quality checked.