mammary mesenchyme rep2.1
Mus musculus
Protocols: Embryonic mammary tissues (embryonic mammary primordial epithelium (MPE), embryonic mammary stroma (includes mammary mesenchyme (MM) and fat pad precursor (FPP)), and surface epithelium (Epi), were micro-dissected from E12.5 B6 mice following the protocol described in Sun L, Lee MY, & Veltmaat JM (2011) A non-enzymatic microsurgical dissection technique of mouse embryonic tissues for gene expression profiling applications. Int J Dev Biol 55(10-12):969-974. Total RNA from mammary tissues were extracted using RNeasy Mini kit 74104, Qiagen, Manchester, UK), Amplified cDNA from embryonic mammary tissue was generated by the SMARTer amplification kit. The cDNA was end-repaired, A-tailed, adapter-ligated and amplified. The prepared libraries were size selected, multiplexed and quality checked.