Adults 17 days old RNA-Seq
Drosophila melanogaster
CS flies were reared at room temperature (23-24C) for collection.  Virgins and young males were collected into a seperate vials and aged 48 hours, separate collections were then made.  Whole body samples were homogenized in 1ml of Trizol (Invitrogen, Carlsbad, California, USA) per 50-100 mg of tissue using a glass-Teflon homogenizer.  RNA was purified according to the suggested manufacturer's protocol (Invitrogen).  RNA was mixed in equimolar amounts and sequenced on the Illumina GA II instrument for 75bp paired-end reads.