Female adult sample from Metisa plana
Metisa plana
Total RNA was exctracted from female adult stage of Metisa plana using RNeasy Mini Kit according to manufacturer's protocol with slight modification . Poly(A) mRNA was then isolated from the total RNA and cDNA library was constructed using NEB Next Ultra RNA Library Preparation Kit . The cDNA library was then sequenced using Illumina HiSeq 4000 according to manufacturer's protocol.