leaves, 3d, full nutrition, harvest 1, rep 2
Solanum lycopersicum
Seeds were germinated in a phytotron and seedlings were transferred to hydroponic boxes containing Hoagland Medium for two weeks, at which point they were transferred to their treatment medium (Hoagland solution, or Hoagland solution with the ammoniumdihydrogenphosphate replaced by equimolar amount of ammonium sulfate). Leaves or roots were harvested and frozen in liquid nitrogen. Total RNA was prepared using MasterPure TM Plant RNA Purification Kit (Epicentre Technologies) for leaf samples or RNeasy Plant Mini Kit (Qiagen) followed by Baseline ZERO DNase digestion (Epicentre Technologies) for root samples. Sequencing libraries were prepared with TruSeq Stranded mRNA Sample Preparation Kit (Illumina) and sequenced on the Illumina NextSeq Platform as single end 75bp reads (NextSeq 500/550 High Output v2 kit, 75 cycle).