pyrosequencing of 16S rRNA gene amplicons of phylloepiphytic bacteria of packaged spinach (day15/10C)
metagenome
Fresh spinach was harvested and packaged to mimic minimally processing.  Spinach was held at 10C for 15 days to mimic temperature abuse, which can occur during retail conditions. Nucleic acids from spinach phyllosphere were isolated and the V4 hypervariable region of the 16S rRNA gene was amplified using RDP primers and analyzed using RDP pyrosequencing pipeline.