pyrosequencing of 16S rRNA gene amplicons of phylloepiphytic bacteria of packaged spinach (day1/4C)
metagenome
Fresh spinach was harvested and packaged to mimic minimally processing.  Spinach was held at 4C for 1 day. Nucleic acids from spinach phyllosphere were isolated and the V4 hypervariable region of the 16S rRNA gene was amplified using RDP primers and analyzed using RDP pyrosequencing pipeline.