A library of tagged ALDOB haplotypes
synthetic construct
Synthetic templates representing mutagenized versions of an enhancer located in the intron of human ALDOB gene were cloned into a plasmid (Promega pGL4.23) upstream of the luciferase gene. The 3' UTR of the luciferase gene was engineered to include a 20bp fully degenerate subsequence to serve as a unique tag to identify each mutant haplotype.