In vitro activated B cells overexpressing AID (IgkAID) and missing 53BP1 (53BP1-/-)
Mus musculus
CD43- resting B cells were isolated from mouse spleens by negative selection with magnetic beads (Miltenyi Biotech). Following isolation, resting B cells were stimulated with LPS (50 mcg/ml) and IL-4 (2.5 ng/ml) for 72 hrs.