Atlantic salmon skin RNA
Salmo salar
Eight salmon (300-400g) reared in salt water and eight parr (~150g) from freshwater were  randomly selected for sampling and humanely killed with a sharp blow on the head. Skin samples  (&lt;100mg) were collected from the dorsal-anterior site of the fish, transferred to 1ml RNAlater  solution (Applied Biosystems, Warrington, UK) and stored at 4*C for 24 h and then at -80*C until  RNA extraction. Total RNA was extracted from the skin samples using the TRI reagent according to the  manufacturer’s recommendations. The final sample used for Roche 454 sequencing was an equimolar mixture of all these samples.