Chlamydomonas reinhardtii
Chlamydomonas reinhardtii pooled D66, cc1690 and cc125 sulfur and phosphorus starvation
strain
D66,cc1690,cc125
in vivo conditions
TAP medium depleted of phosphorous or sulfur; continuous light and shaking; 23 degC
library_type
RAPID
library_description
C. reinhardtii strains D66 cells were grown in modifed TAP medium with -S at 23 Celsius, 150 RPM shaking, and under continuous light at 90 µmol/m^2*s. RNA was isolated from cells collected at a cell density of 5.0x10^6 cells/ml. C. reinhardtii strains CC-1690 and CC-125 were grown in modifed TAP medium with -P at 25 Celsius, 200 RPM shaking, and under continuous light at 70 µmol/m^2*s. Cells were inoculated into TAP medium at a chlorophyll density of 2.5 mg/ml. RNA was collected from cells after 24 hours of growth. RNA samples were pooled in equal proportion for sequencing. Sample provided by Arthur Grossman.