sediment metagenome
DNA was extracted with the Fast DNA Spin Kit for Soil (MP Biomedicals). A two-step PCR was performed to amplify the nitrous oxide reductase genes of nosZ  clade I and II. Pyrosequencing was performed by Microsynth (Switzerland) and sequence data was initially screened and de-multiplexed using QIIME (Caporaso et al., 2010) with the default parameters.
isolation_source
Silty mud sediment from a shallow coastal bay
collection_date
2013
geo_loc_name
Sweden: west coast
lat_lon
missing
BioSampleModel
Metagenome or environmental