Drosophila melanogaster
ovaries, separated at the oviduct
strain
w[1118] FBst0005905
age
6-8 hours post eclosion
dev_stage
6-8 hr post-eclosion, virgin
sex
female
tissue
gonads
biomaterial_provider
Brian Oliver Lab
genotype
w[1118]
growth_protocol
We grew flies on Fly food A (LabExpress Ann Arbor, MI) at 25C in constant light and 60% relative humidity, and collected from virgin males 6-8 hrs post-eclosion.
phenotype
white eyes
sample_type
RNA
store_cond
-80 oC
treatment
reference
sample_role
reference
temperature_regimen
25 oC
details
We grew flies on Fly food A (LabExpress Ann Arbor, MI) at 25C in constant light and 60% relative humidity, and collected from virgin females 6-8 hrs post-eclosion. We dissected in PBS pH 7.4 (Gibco Life Technologies, Carlsbad, CA) at 22 C. We separatged ovaries from the reproductive tract near oviduct junction. Sample (N = 50) was collected in 350 l RLT buffer (Qiagen, Cat. No. 79216. Valencia, CA), vortexed quickly and frozen in a dry ice ethanol bath and stored at -80 C until total RNA extraction using the RNeasy Mini kit (Qiagen, Cat. No. 74104. Valencia, CA) following the manufacturers protocol. RNA was quantified using Quant-iT RiboGreen quantification kit (Life Technologies, Cat. No. R11490. Carlsbad, CA). RNA was stored in -80 C.
BioSampleModel
Model organism or animal