Pogonus chalceus
Beetles of P. chalceus were captured in the Gu*rande region, France. Eggs were obtained from canal ecotype individuals and raised in a common environment. A larva (third-instar), pupa and imago (male) resulting from the same mother were frozen in liquid nitrogen and subsequently used for sequencing. Total RNA was isolated from a complete pupa. RNA was extracted using the SV Total RNA isolation System (Promega, Madison, USA) according to manufacturer's instructions and genomic DNA was removed by on-column digest with DNase I.