Homo sapiens
Protocols: MDA-MB-453 and MCF7 cells were grown in DMEM containing 10% heat-inactivated FBS, 2 mM L glutamine, 50 U/ml penicillin and 50 ug/ml streptomycin and LNCaP cells were grown in RPMI containing 10% heat-inactivated FBS, 2 mM L glutamine, 50 U/ml penicillin and 50 ug/ml streptomycin. The antibodies used were anti-AR (sc-816) and anti-ER (sc-543) from Santa Cruz Biotechnologies and anti-FoxA1 (ab5089) from Abcam. Four 15 cm dishes of cells were used per chromatin immunoprecipitation (ChIP) and samples were processed according to standard ChIP procedures as described in Schmidt et al, Methods 48(3) July 2009 doi:10.1016/j.ymeth.2009.03.001. The immunoprecipitated DNA was subsequently amplified as previously described for Illumina sequencing (Schmidt et al, 2009).
ENA-FIRST-PUBLIC
2012-02-18T17:00:29Z
ENA-LAST-UPDATE
2018-03-08T15:36:05Z
External Id
SAMEA1094833
INSDC center name
CRUK-CRI
INSDC first public
2012-02-18T17:00:29Z
INSDC last update
2018-03-08T15:36:05Z
INSDC status
public
Submitter Id
E-MTAB-986:LNCaP
broker name
ArrayExpress
cell line
LNCAP
cell type
epithelial
common name
human
organism part
prostate
sample name
E-MTAB-986:LNCaP
scientific_name
Homo sapiens
sex
male