freshwater sediment metagenome
The sediment were collected from Chenghai Lake, which is located in Yunnan province of China. Total DNA was extracted from 1.0 g mud using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) following the manufacturer's instructions. The target region of 16S rRNA gene were amplified after PCR, and the pyrosequencing was carried out on a 454 Life Sciences Genome Sequencer FLX instrument (Roche) in Shanghai Majorbio Bio-pharm Biotechnology Co., China.