Chlamydomonas reinhardtii
Chlamydomonas reinhardtii 6145C mitotic samples
strain
6145C
developmental_stage
mitosis
in vivo conditions
mitotic
library_type
RAPID
library_description
C. reinhardtii strain 6145C cells were grown in HSM medium with a light/dark cycle of 14 hours light/ 10 hours dark. RNA from 3 samples representing different stages of mitosis (at 13 hrs light, 13.5 hrs light, and 0.5 hrs dark) were prepared using TRIzol followed by DNase treatment and RNeasy column purification. Equal masses of RNA from the three samples were pooled for sequencing. Sample provided by James Umen and Matt Zones.