Anomotaenia brevis
Infected colonies of the ant T. nylanderi were collected in the years 2013 - 2015 in the Lenneberg forest and Eltville near Mainz, Germany. In January 2016, 40 infected workers from 18 different colonies were dissected, resulting in a total of 329 isolated cysticercoids that were washed three times in DNase-free water to remove remains of ant tissue. RNA extractions were performed in four rounds so that dissections of cestodes for one round did not last longer than one hour. Cestodes per round were transferred into 100 microliter of Trizol and RNA extraction was performed using the RNAeasy mini extraction kit (Qiagen) and standard protocol. Afterward, samples were combined and sent to BGI Hongkong for RNA sequencing on an Illumina HiSeq 4000.
isolate
pool of 329 cestodes dissected from infected ants of Temnothorax nylanderi
host
Temnothorax nylanderi
collection_date
2013-12/2015-07
geo_loc_name
Germany:Lennebergwald and Kloster Eberbach (Eltville), Mainz
tissue
whole body
biomaterial_provider
AG Foitzik, Johannes Gutenberg University Mainz, Germany
collected_by
Sara Beros
dev_stage
cysticercoid
host_tissue_sampled
ant abdomen
sex
not determined
BioSampleModel
Invertebrate