Homo sapiens
Protocols: total peripheral blood cells were collected, AML-M0 blast cells represent 87% of total population DNA was extracted using Qiagen Kit The fragment ends were repaired, “A” bases were added ends of the DNA fragments and adapters were ligated to the fragments. The adapter-ligated library was amplified using 4 to 6 cycles of PCR. 500 nanograms of the sample library were hybridized with the SureSelect biotinylated RNA library for 24 hours. Bound DNA was purified using streptavidin coated magnetic beads
ENA first public
2017-07-05
ENA last update
2016-05-05
External Id
SAMEA3951804
INSDC center alias
INSERM U1170 Gustave Roussy
INSDC center name
INSERM U1170 Gustave Roussy
INSDC first public
2017-07-05T17:02:04Z
INSDC last update
2016-05-05T09:21:03Z
INSDC status
public
Submitter Id
E-MTAB-4679:SC_LAM
age
43
broker name
ArrayExpress
cell type
blood cell
common name
human
disease
Familial platelet syndrome with predisposition to acute myelogenous leukemia
disease staging
acute myeloid leukemia-M0 (AML-M0)
organism part
blood
sample name
E-MTAB-4679:SC_LAM
sex
male