Mus musculus
Protocols: Primary fibroblasts were derived from adult CAST/EiJ x C57/Bl6 mice. Tail explants were cultured in fibroblast medium (DMEM high glucose, 10% ES cell FBS, 1% Penicillin/Streptomycin, 1% non-essential amino acids, 1% sodium-puryvate, 0.1mM b-Mercaptoethanol). Cells were passaged twice before distributed as individual cells into wells of 384 or 96 well plates using a FACS. Wells was pre-loaded with lysis buffer. ES cells were cultured as in Meissner et al. Methods Mol Biol 2009, and similarly distributed via FACS into as single cells into wells of 96 and 384-well plates Cell lysis. Smart-seq2, indexed by Nextera i5 and i7 and with a unique molecular identifier present in template-switching oligonucleotide. No exogenous spike-ins were used..
ENA-FIRST-PUBLIC
2018-11-30T11:01:58Z
ENA-LAST-UPDATE
2018-07-30T11:05:06Z
External Id
SAMEA4808982
INSDC center name
Karolinska Institutet, Department of Cell and Molecular Biology
INSDC first public
2018-11-30T11:01:58Z
INSDC last update
2018-07-30T11:05:06Z
INSDC status
public
Submitter Id
E-MTAB-7098:Sample 454
broker name
ArrayExpress
cell type
mouse embryonic stem cell
common name
house mouse
developmental stage
embryo
genotype
wild type genotype
organism part
inner cell mass
sample name
E-MTAB-7098:Sample 454
scientific_name
Mus musculus
sex
male
strain
C57BL/6 x CAST/EiJ