Homo sapiens
Protocols: Peripheral blood was collected from patients with primary myelofibrosis (PMF) or post ET- or PV-myelofibrosis (MF). Patients were part of of the MPNSG-0109 study (Pomalidomide in myeloproliferative neoplasm-associated myelofibrosis. Schlenk RF, Stegelmann F et al., Leukemia 2017). HREC approval 45/07 Universität Ulm Ethikkommission. Most patients were untreated at sample collection, apart from two patients treated with Pomalidomide and one patient treated with Hydroxyurea. RNA was extracted RNA libraries were prepared using the NEBnext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), assessed for size and quantified using the 2100 Bioanalyzer (Agilent Technologies) and Kapa Library Quantitation Kit (Illumina) respectively.
ENA-FIRST-PUBLIC
2018-12-01T17:02:15Z
ENA-LAST-UPDATE
2018-10-01T09:55:40Z
External Id
SAMEA4947838
INSDC center name
Gordon and Jessie Gilmour Leukaemia Research Laboratory, QIMR Berghofer Medical Research Institute
INSDC first public
2018-12-01T17:02:15Z
INSDC last update
2018-10-01T09:55:40Z
INSDC status
public
Submitter Id
E-MTAB-7255:10PB6194
age
51
asxl1 gene
mutation
broker name
ArrayExpress
calr gene
wild type
clinical history
primary myelofibrosis
common name
human
disease
primary myelofibrosis
dnmt3a gene
c.A89c.pE30A
genotype
JAK2VF DNMT3A ASXL1
jak2vf gene
mutation
mpl gene
wild type
organism part
blood
sample name
E-MTAB-7255:10PB6194
scientific_name
Homo sapiens
sex
female
srsf2 gene
wild type
tet2 gene
wild type
treatment
pomalidomide