Homo sapiens
Protocols: HeLa cells were washed in cold PBS and harvested by scraping into cold PBS followed by centrifugation at 300 x g. DNA from up to 10^6 cells was extracted using a Quick-DNA™ kit (Zymo Research) according to the manufacturer's instructions. Genomic lysis buffer was supplemented with TEMPO (20 mM) to minimise the introduction of oxidative damage and DNA was eluted in Tris-HCl buffer (pH 7.4, 10 mM). Input libraries were prepared for each enriched (snAP-seq) library. DNA was end-prepped using an NEBNext Ultra II kit, followed by adapter ligation using Truseq LT indexed adapters (Illumina). PCR amplification conditions were kept consistent with corresponding enriched (snAP-seq) libraries.
ENA-FIRST-PUBLIC
2019-06-12T17:06:27Z
ENA-LAST-UPDATE
2018-09-04T14:35:11Z
External Id
SAMEA4883112
INSDC center name
Department of Chemistry University of Cambridge
INSDC first public
2019-06-12T17:06:27Z
INSDC last update
2018-09-04T14:35:11Z
INSDC status
public
Submitter Id
E-MTAB-7152:APE1-siRNA_input4
broker name
ArrayExpress
cell line
HeLa
cell type
epithelial cell
common name
human
disease
cervical adenocarcinoma
genotype
wild type genotype
organism part
uterine cervix
sample name
E-MTAB-7152:APE1-siRNA_input4
scientific_name
Homo sapiens