Mus musculus
Protocols: BLaER1 cells are as described here: https://doi.org/10.1016/j.celrep.2013.03.003 and are derived as a subclone of Seraphina cells CVCL_M646. C10 cells are as described https://doi.org/10.1016/j.stem.2009.10.004 and are derived as a subclone of the transformed fetal liver HAFTL-1 cell line. Fluorescent staining of cell surface markers was done with antibodies against Mac1 (APC) and CD19 (APC-Cy7) (BD Pharmigen). Samples were analyzed on the LSRII flow cytometer (BD Biosciences) and data were analyzed with FlowJo software (Tree Star). Human BLaER1 cells and mouse C10 cells were grown in RPMI (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, and 100 U/ml penicillin G sodium. Transdifferentiation was induced in BlaER1 and C10 cells by addition of 100nM of β-estradiol (Calbiochem) and grown with 10nM of IL-3 and CSF-1 (Peprotech). Cells were lysed with Qiazol following the manufacturer's instructions (Qiagen). Chloroform was added to each sample and RNA contained in the aqueous fraction was isolated and purified by using RNeasy mini kit columns also following the manufacturer's instructions (Qiagen). Stranded poly A+ libraries were prepared with 1 ug of total RNA using TruSeq mRNA Sample Prep Kit (Illumina) according to manufacturer's protocol.
ENA checklist
ERC000011
INSDC center alias
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Bioinformatics and Genomics.
INSDC center name
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Bioinformatics and Genomics.
INSDC first public
2018-11-30T11:01:58Z
INSDC last update
2018-08-03T10:51:40Z
INSDC status
public
SRA accession
ERS2634181
Sample Name
ERS2634181
alias
E-MTAB-7115:Mouse_003h
broker name
ArrayExpress
cell line
C10
cell type
precursor B cell
disease
normal
organism
Mus musculus
organism part
liver
title
Mouse_003h