Danio rerio
Protocols: 8 dpf wildtype and Tg(twhh:Gal4);Tg(UAS:EGFP-HRASV12) were euthanized with 3% Tricaine methanesulfonate (Sigma). Embryos were decapitated and incubated in Tripsin-EDTA (Sigma) for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol-LS (Ambion). We isolated 30-50 notochords per replicate, with a total of 3 replicates per condition (3x wildtype, 3x HRASV12). Notochord RNA was extracted following the manufacturer's protocol using Trizol-LS. RNA-seq libraries were constructed using the Truseq stranded total RNA kit.
ENA-FIRST-PUBLIC
2018-12-01T17:03:21Z
ENA-LAST-UPDATE
2018-10-30T13:30:13Z
External Id
SAMEA5055150
INSDC center name
UZH
INSDC first public
2018-12-01T17:03:21Z
INSDC last update
2018-10-30T13:30:13Z
INSDC status
public
Submitter Id
E-MTAB-7349:TUA
age
8
broker name
ArrayExpress
common name
zebrafish
developmental stage
embryo
genotype
wild type genotype
individual
mixed pool of 30 to 50 embryos
organism part
notochord
sample name
E-MTAB-7349:TUA
scientific_name
Danio rerio
sex
mixed