Drosophila melanogaster
testes and vas deferens, separated at the seminal vessicle
strain
w[1118] FBst0005905
age
5-7 days post eclosion
dev_stage
adult, mated
sex
male
tissue
gonads
biomaterial_provider
Brian Oliver Lab
genotype
w[1118]
growth_protocol
We grew flies on Fly food A (LabExpress Ann Arbor, MI) at 25C in constant light and 60% relative humidity, and collected from females 5-7 days post-eclosion. Flies mated ad librum.
phenotype
white eyes
sample_type
RNA
store_cond
-80 oC
treatment
reference
sample_role
reference
temperature_regimen
25 oC
details
We grew flies on Fly food A (LabExpress Ann Arbor, MI) at 25C in constant light and 60% relative humidity, and collected from males 5-7 days post-eclosion. Flies mated ad librum. We dissected in PBS pH 7.4 (Gibco Life Technologies, Carlsbad, CA) at 22 C. We separated testes and vas defrens at the junction with the seminal vesicle. Sample (pooled N = 100) was collected in 350 l RLT buffer (Qiagen, Cat. No. 79216. Valencia, CA), vortexed quickly and frozen in a dry ice ethanol bath and stored at -80 C until total RNA extraction using the RNeasy Mini kit (Qiagen, Cat. No. 74104. Valencia, CA) following the manufacturers protocol. RNA was quantified using Quant-iT RiboGreen quantification kit (Life Technologies, Cat. No. R11490. Carlsbad, CA). RNA was stored in -80 C.
BioSampleModel
Model organism or animal