Homo sapiens
Protocols: OE33 cells were cultured in RPMI 1640 medium containing 2 mM glutamine and 10% Fetal Bovine Serum. After cross-link reversal and protein digestion with Proteinase K (Roche), DNA was purified using Qiaquick, PCR purification kit (Qiagen) following the manufacturer's instructions. ChIP was performed as described in Wiseman et al., 2015 using 3x10^7 cells. Immunoprecipitation was performed overnight at 4°C with 3 μg antibody (anti-ETV1) and 30 μl Dynabeads per experiment. Library preparation was performed using the TruSeq ChIP Sample Preparation Protocol (Illumina). Wiseman EF, Chen X, Han N, Webber A, Ji Z, Sharrocks AD, Ang YS. Deregulation of the FOXM1 target gene network and its coregulatory partners in oesophageal adenocarcinoma. Mol Cancer. 2015 Mar 26;14:69. doi: 10.1186/s12943-015-0339-8. Erratum in: Mol Cancer. 2016;15:21. PubMed PMID: 25889361.
ENA first public
2017-07-07
ENA last update
2016-10-19
External Id
SAMEA4518221
INSDC center alias
UOM
INSDC center name
University of Manchester
INSDC first public
2017-07-07T17:02:49Z
INSDC last update
2016-10-19T11:26:13Z
INSDC status
public
Submitter Id
E-MTAB-5168:ETV1 IP rep1
broker name
ArrayExpress
cell line
OE33
cell type
epithelial cell
common name
human
disease
esophageal adenocarcinoma
organism part
esophagus
sample name
E-MTAB-5168:ETV1 IP rep1
sex
female