Bos taurus
DNAmixture of 44 semens - The sample was the mixture of DNA extracted from 44 Chinese Holstein bulls semen
Common Name
cattle
Sample Name
pooled DNA
Scientific Name
Bos taurus
Sex
male
Submission description
We designed and synthesized 23 pairs of primers in the entire exons, and 3 kb of 5′ and 3′ flanking regions of bovine LIPJ gene according to the genomic sequences of (GenBank accession number: NC_037353.1). 44 semen DNAs were pooled to use for the PCR amplifications with the equal concentration (50 ng/µL) for each DNA. All the PCR products were sequenced by ABI3730XL DNA analyzer (Applied Biosystems, Foster, CA, USA). Then, we aligned the sequences with the reference genome (UMD3.1.1) using the BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi), and identified the novel SNP, g.10250120A/G.
Submission identifier
GSB-702
Submission title
the National Science and Technology Programs of China
collected_by
Dongxiao Sun