Phalaenopsis aphrodite
Vegetative library with pooled RNAs from root (1 cm from root tip), stem and leaf was constructed by SMART cDNA Synthesis kit (Clontech, CA, USA) and normalized via DSN (Duplex-specific nuclease, Evrogen, Russia) (Zhulidov et al., 2004) treatment followed by pair-end DNA Sample Preparation Kits (Illumina, CA, USA) which included DNA shearing by sonication, adaptor ligation and DNA enrichment.
tissue
flower