Anopheles gambiae
Protocols: The Anopheles gambiae Ngousso strain was originally initiated with mosquitoes collected in Cameroon in January 2006, and belongs to the M molecular and Forest chromosomal forms (Harris C, Lambrechts L, Rousset F, Abate L, Nsango SE, Fontenille D, Morlais I, Cohuet A. (2010). Polymorphisms in Anopheles gambiae immune genes associated with natural resistance to Plasmodium falciparum. PloS Pathog 6, e1001112.). Mosquito were reared under standard conditions as previously described (Mitri C, Jacques JC, Thiery I, Riehle MM, Xu J, Bischoff E, Morlais I, Nsango SE, Vernick KD, Bourgouin C. (2009). Fine pathogen discrimination within the APL1 gene family protects Anopheles gambiae against human and rodent malaria species. PloS Pathog 5, e1000576. Mosquitoes were infected on an infectious bloodmeal containing physiologically natural viral titers O'Nyong-Nyong strain SG650 12 mosquitoes per pool, in each of two replicates, were collected 3 days after bloodmeal infection with ONN and homogenized in TriReagent (Sigma). Small RNAs fraction (<200 nt) were extracted using Nucleospin miRNA (Machery Nagel) using the Trizol protocol and submitted to Bioanalyser for quality assessment. Briefly, the 10- to 30-nt RNA fragments were purified from small RNA fraction on a 15% urea PAGE (Biorad). Small RNA library were prepared using TruSeq Small RNA Library reagents (Illumina).
ENA-FIRST-PUBLIC
2015-05-22T17:38:28Z
ENA-LAST-UPDATE
2018-03-08T17:42:17Z
External Id
SAMEA2547935
INSDC center name
Institut Pasteur
INSDC first public
2015-05-22T17:38:28Z
INSDC last update
2018-03-08T17:42:17Z
INSDC status
public
Submitter Id
E-MTAB-2595:Anopheles gambie infected by onnv (replicate 1)
broker name
ArrayExpress
common name
African malaria mosquito
developmental stage
adult
genotype
wild type
infect
o'nyong'nyong virus
phenotype
infected
sample name
E-MTAB-2595:Anopheles gambie infected by onnv (replicate 1)
sampling time
3 days after bloodmeal
scientific_name
Anopheles gambiae
sex
female
specimen with known storage state
fresh specimen
strain
Ngousso