Homo sapiens
Protocols: The OE33 cells were cultured in RPMI media supplemented with 10% foetal bovine serum. To generate samples for RNA-seq analysis, OE33 cells (3 biological replicates) were transduced with lentiviruses expressing a dominant-negative (DN) FOS (DN-FOS) construct (pInducer-DN-FOS) or lacking an insert (control)(pInducer) and grown for a further 12 hours. Following transfection cells were treated with doxycycline (100 ng/ml) for 48 hours to induce DN-FOS expression and GFP positive cells were selected before harvesting RNA. RNA was extracted using RNeasy (Qiagen) according to the manufacturer's protocol. cDNA libraries were prepared following the TruSeq Stranded mRNA sample preparation protocol (Illumina)
ENA first public
2017-07-07
ENA last update
2016-10-11
External Id
SAMEA4503857
INSDC center alias
UOM
INSDC center name
University of Manchester
INSDC first public
2017-07-07T17:02:48Z
INSDC last update
2016-10-11T15:30:58Z
INSDC status
public
Submitter Id
E-MTAB-5175:control_2
broker name
ArrayExpress
cell line
OE33
cell type
epithelial cell
common name
human
disease
esophageal adenocarcinoma
genotype
pInducer
organism part
esophagus
phenotype
wild type
sample name
E-MTAB-5175:control_2