Mus musculus
Male C57BL/6JN and B6D2F1 mice fed either ad libitum or CR were obtained from the aging colony maintained by the National Institute on Aging (NIA).  After receiving the mice from NIA were housed at the animal facility and maintained under SPF conditions and individually housed in a HEPA barrier environment at the University of Oklahoma Health Sciences Center for at 4-8 weeks before used in the following experiments. Mice fed ad libitum were fed irradiated NIH-31 mouse/rat diet (Teklad, Envigo), and the CR mice were fed the same diet fortified for micronutrients. CR was initiated by NIA at 14 weeks of age of 10% restriction, increased to 25% restriction at 15 weeks, and to 40% restriction at 16 weeks of age. Adult (9 months of age) fed ad libitum (AL) or CR and old mice (24 months of age) fed AL or CR were fasted overnight, and samples were collected during sacrifice (n=8-10/group).
strain
C57BL/6
age
9 months
sex
male
tissue
Colon mucosa
treatment
ad libitum diet
replicate
ACR4
BioSampleModel
Model organism or animal