Chilo suppressalis
The Chilo suppressalis strain was collected from the farm at the Huazhong Agricultural University, Wuhan, Hubei, China in 2010. This strain was reared in plastic containers (15 cm diameter * 8 cm high) on an artificial diet at 28 * 1 *C under a 16 h photoperiod and &gt; 80% relative humidity. For total RNA and protein preparation, midguts were dissected from fifth instar larvae and cleaned in ice-cold 0.7% NaCl solution, immediately frozen in liquid nitrogen, and then stored at -80 *C until use. The midgut content was removed before processing.