Homo sapiens
Protocols: cutaneous biopsy was performed and fibroblasts were culture in MEM medium supplementend with 10%FBS; penicilline, streptomycine and Fungizone. DNA was extracted using Qiagen Kit The fragment ends were repaired, “A” bases were added ends of the DNA fragments and adapters were ligated to the fragments. The adapter-ligated library was amplified using 4 to 6 cycles of PCR. 500 nanograms of the sample library were hybridized with the SureSelect biotinylated RNA library for 24 hours. Bound DNA was purified using streptavidin coated magnetic beads
ENA first public
2017-07-05
ENA last update
2016-05-05
External Id
SAMEA3951802
INSDC center alias
INSERM U1170 Gustave Roussy
INSDC center name
INSERM U1170 Gustave Roussy
INSDC first public
2017-07-05T17:02:04Z
INSDC last update
2016-05-05T09:21:03Z
INSDC status
public
Submitter Id
E-MTAB-4679:SC_Fib
age
41
broker name
ArrayExpress
cell type
fibroblast
common name
human
disease
Familial platelet syndrome with predisposition to acute myelogenous leukemia
disease staging
thrombopenia
organism part
skin
sample name
E-MTAB-4679:SC_Fib
sex
male