Zea mays
isolate
400-2
cultivar
W22
dev_stage
mid-prophase meiotic-staged male flowers from 6-8 week old plants
geo_loc_name
USA: Tallahassee, FL
tissue
Tassel
genotype
The wild-type W22 used in this study is a color-converted W22 line obtained from Hugo Dooner (Waksman Inst., Rutgers, New Jersey, USA) derived by Brink (1956). The UF-Mu-00395 seed stock was obtained from the Maize Genetics Cooperation Stock Center (http://maizecoop. cropsci.uiuc.edu/). In the fall of 2018, the progeny seeds were grown in the greenhouse in the King Life Sciences building (Biological Science Dept, Florida State University, Tallahassee, FL, USA). The segregating plants were self-crossed to obtain mutant plants from among the progeny.
growth_protocol
Segregating wildtype and mutant mkaku41 plants were grown in the greenhouse. From two week-old plants, fourth leaves were harvested and from 6-8 week old plants, mid-prophase meiotic-staged male flowers were harvested. The tissues were immediately stored in liquid nitrogen. RNA was isolated from three biological replicates for each genotype using Qiagen RNeasy Plant mini kit as per manufacturers instructions.
BioSampleModel
Plant