Mus musculus
Protocols: T helper type 2 cells were extracted from mouse spleens and activated with CD3/CD28. Each mouse replicate is a separate purification of T cells from a collection of mice. Mice were 8-12 weeks old and of mixed gender. Cells were spinoculated with retroviruses overexpressing a gene of interest on day 1. IL4 is 10ng/ml, and the cells were in treated with this for the remainder of the time spent in culture. For the overexpression for chipseq and rnaseq you can refer to Addgene plasmids #117263-117267. On day 4, BFP-positive (infected) cells were FACS sorted and measured by a variant of SMART-seq2. Further information can be found in the preprint here: https://www.biorxiv.org/content/early/2017/12/28/196022 or E-MTAB-6285. Lrrc40dec is a truncated version of Lrrc40. Cells were lysed with RLT+ and RNA purified using ampure XP beads A modified version of SMARTseq2
ENA-FIRST-PUBLIC
2018-11-27T17:03:40Z
ENA-LAST-UPDATE
2018-09-20T10:02:50Z
External Id
SAMEA4925780
INSDC center name
Sanger Institute / Karolinska Institutet
INSDC first public
2018-11-27T17:03:40Z
INSDC last update
2018-09-20T10:02:50Z
INSDC status
public
Submitter Id
E-MTAB-7260:Sample 1
age
8 to 12
broker name
ArrayExpress
cell type
T-helper 2 cell
common name
house mouse
genotype
MSCV-Lrrc40dec-3xFLAG-IRES-BFP
individual
mixed pool 3
organism part
spleen
phenotype
Lrrc40dec overexpression
sample name
E-MTAB-7260:Sample 1
scientific_name
Mus musculus
sex
mixed
stimulus
CD3/CD28
strain
CBLA