Acyrthosiphon pisum
Replicate 2 : Acyrthosiphon pisum individuals (strain RR2) were reared on broad bean Vicia fabae at low density (less than five individuals per plant) to prevent the production of winged morphs. Biological material was prepared under long photoperiod condition (at 16h of light) that maintains asexual reproduction, at constant temperature of 18°C. Parthenogenetic females were moved at L3 stage and some of their offspring (G1) were collected at L2 stage. Around 25 individuals per sample were collected and frozen in liquid nitrogen for further heads dissection. Duplicates were performed ended up with 8 samples for which total RNAs were extracted using the SV Total RNA Isolation System (Promega) according to manufacturer's instructions. RNA quality was checked on Bioanalyzer (Agilent) and quantified on Nanodrop (Thermo Scientific).
replicate
L2G1-NI rep2
strain
RR2
dev_stage
L2
sex
not determined
tissue
head
biomaterial_provider
Gaël Le Trionnaire (INRA, UMR 1349, France)
BioSampleModel
Model organism or animal