Saccharomyces cerevisiae
Protocols: For each strain, three independent colonies were used to prepare samples of total RNA. Wild-type H4 and otherwise isogenic T80A H4 cells were grown in yeast extract, peptone, glucose, adenine sulfate (YPDA) to an optical density at 660 nm (OD660) of 0.6. Total RNA was isolated through hot-phenol extraction. RNA was further subjected to DNase I treatment (catalog number E1009-A; Zymo Research) and poly-A mRNA was purified using poly-T oligo-attached magnetic beads (NEXTflex Poly(A) Beads, catalog number NOVA-512981) according to the manufacturer's instructions. The quantity and purity of RNA were measured by using an Agilent high-sensitivity RNA screen tape system (catalog number 5067-5579; Agilent Technologies) and Qubit (Molecular Probes, Invitrogen). Libraries were prepared for sequencing from 200 ng of polyA mRNA by using the NEXTflex RNA-seq kit (NOVA-512913, Illumina) according to the manufacturer's instructions. Samples were barcoded and combined at uniform molarity to create a single pool for sequencing.
ENA-FIRST-PUBLIC
2018-09-05T17:03:05Z
ENA-LAST-UPDATE
2018-07-30T11:20:33Z
External Id
SAMEA4809027
INSDC center name
University of Portsmouth
INSDC first public
2018-09-05T17:03:05Z
INSDC last update
2018-07-30T11:20:33Z
INSDC status
public
Submitter Id
E-MTAB-7090:H4T80A.Rep1
broker name
ArrayExpress
common name
baker's yeast
genotype
H4T80A
growth condition
YPDA medium
sample name
E-MTAB-7090:H4T80A.Rep1
scientific_name
Saccharomyces cerevisiae