Homo sapiens
Protocols: OE19 cells were cultured in RPMI media supplemented with 10% foetal bovine serum. HEEPIC cells were grown on poly-l-lysine coated plates (2 μg/cm2) in epithelial cell media supplemented with 1% epithelial growth supplement. Biopsy tissue samples (~4mm) were processed as described previously (Wiseman et al., 2015). Wiseman EF, Chen X, Han N, Webber A, Ji Z, Sharrocks AD, Ang YS. Deregulation of the FOXM1 target gene network and its coregulatory partners in oesophageal adenocarcinoma. Mol Cancer. 2015 Mar 26;14:69. doi: 10.1186/s12943-015-0339-8. Erratum in: Mol Cancer. 2016;15:21. PubMed PMID: 25889361. Adherent cells were first detached using accutase cell detachment solution, washed with complete growth media containing 10% foetal bovine serum, pelleted and resuspended in 1x Dulbecco's PBS. Cell numbers were quantified and 50,000 cells taken forward to the nuclei preparation step of the ATAC-seq protocol as described in Buenrostro, J. D., Giresi, P. G., Zaba, L. C., Chang, H. Y., & Greenleaf, W. J. (2013). Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nature Methods, 10(12), 1213-1218. http://doi.org/10.1038/nmeth.2688
ENA first public
2017-07-07
ENA last update
2016-10-19
External Id
SAMEA4518228
INSDC center alias
UOM
INSDC center name
University of Manchester
INSDC first public
2017-07-07T17:02:49Z
INSDC last update
2016-10-19T11:33:41Z
INSDC status
public
Submitter Id
E-MTAB-5169:s1_002-T
broker name
ArrayExpress
cell line
biopsy
cell type
epithelial cell
common name
human
disease
esophageal adenocarcinoma
organism part
esophagus
sample name
E-MTAB-5169:s1_002-T
tumor grading
T1N0M0