Mus musculus
Protocols: Lungs were removed from mice and stored in RNAlater solution (Thermo fisher scientific)until being processed. B-16V WT or BAG6KO cells were cultured in CD293 particle-free medium for 48h under hypoxic conditions before EVs were isolated from supernatants by Ultracentrifugation (precentrifugation 5min 300xg, 10 min 2000xg and 20 min 3500xg; ultracentrifugation at 100k x g)Mice were intravenously injected with 20 µg WT BAG6KO EVs in 50µl PBS, or 50µl PBS control, for 4 weeks on a weekly basis. Tissue homogenization of lungs stored in RNAlater solution (Thermo fisher scientific) for total RNA extraction using gentle MACS (Mitenyi biotec) followed by RNA isolation using RNeasy Mini Kit (Qiagen) including on-column DNA digest using RNase-Free DNase Set (Qiagen), RNA elution in H20 Illumina truseq mRNA kit v2
ENA-FIRST-PUBLIC
2018-11-30T11:01:58Z
ENA-LAST-UPDATE
2018-08-08T14:15:42Z
External Id
SAMEA4821600
INSDC center name
Center for Immune and Tumor Biology
INSDC first public
2018-11-30T11:01:58Z
INSDC last update
2018-08-08T14:15:42Z
INSDC status
public
Submitter Id
E-MTAB-7119:ctrl3
age
8 to 12
broker name
ArrayExpress
common name
house mouse
disease
normal
genotype
wild type genotype
organism part
lung
sample name
E-MTAB-7119:ctrl3
scientific_name
Mus musculus
sex
male
strain
C57BL/6J