Chlamydomonas reinhardtii
Chlamydomonas reinhardtii 137c anaerobic dark
strain
137c
in vivo conditions
anaerobic,dark
library_type
RAPID
library_description
"C. reinhardtii strain CC-124 cells were grown in TAP medium at 24 Celsius, 180 RPM shaking, and under continuous light between 30-60 µmol/m^2*s. Cells were grown in 6 x 1 L TAP to density of 1.7x10^6 cells/ml before being concentrated by centrifugation (5min, 2170xg). All pellets were resuspended in a total of 1 L TAP and distributed to two 500 mL bottles. Sealed bottles were kept in the dark. RNA was isolated from cells collected after 45min (bottle 1) and 120min (bottle 2). RNA samples were pooled in equal proportion for sequencing. Sample provided by Sabeeha Merchant and Anja Hemschemeier."