Saccharomyces cerevisiae
Protocols: Of the 115 ml of cultures, 15 ml were collected, centrifuged at 2000 g for 3 min at 4°C, transferred into an Eppendorf and snap frozen in liquid nitrogen for transcriptomic analysis. All media were prepared in a single batch to limit experimental variability. A subset of 129 strains were picked in random series of 16, pre-cultured in in-house made synthetic dextrose medium (S.D., containing per liter: 1.7 g yeast nitrogen base without amino acids (Chemie Brunschwig), 5 g ammonium sulfate, 2% glucose (w/v), 0.03 g isoleucine, 0.15 g valine, 0.04 g adenine, 0.02 g arginine, 0.02 g histidine, 0.1 g leucine, 0.03 g lysine, 0.02 g methionine, 0.05 g phenylalanine, 0.2 g threonine, 0.04 g tryptophan, 0.03 g tyrosine, 0.02 g uracil, 0.1 g glutamic acid and 0.1 g aspartic acid), and then grown in 115 ml fresh S.D. medium at 30ºC until a maximal optical density at 600 nm (OD600) of 0.8 (+/- 0.1). Total RNA was isolated from deep frozen aliquots of yeast pellets using the RiboPure™ RNA Purification Kit, yeast (Ambion), which includes a DNase treatment to eliminate contamination. RNA quality was assessed using RNA ScreenTape assay (Agilent). cDNA libraries were prepared from poly(A) selected RNA applying the Illumina TruSeq protocol for mRNA using a total of 1 μg RNA per sample and 14 PCR cycles.
ENA-FIRST-PUBLIC
2020-07-07T04:03:32Z
ENA-LAST-UPDATE
2019-07-16T17:42:30Z
External Id
SAMEA5778021
INSDC center name
Cluster of Excellence in Cellular Stress Responses in Aging-associated Diseases, Cologne, Germany
INSDC first public
2020-07-07T04:03:32Z
INSDC last update
2019-07-16T17:42:30Z
INSDC status
public
Submitter Id
E-MTAB-8146:LG98
broker name
ArrayExpress
common name
baker's yeast
culture batch
7
growth condition
cell suspension culture
sample name
E-MTAB-8146:LG98
scientific_name
Saccharomyces cerevisiae
strain
14.1.b