Candida albicans
Protocols: Resistance to the antifungal drug was monitored using the E-test assay method (AB biodisk, Biomerieux). The experiment was pursued until strains were growing in liquid media containing a Fluconazole concentration of 256ug/ml (suffix FH) DNA extraction was carried out using the Genomic-tip 100/G kit (Qiagen) according to the manufacturer's protocol. DNA quantity and integrity was assessed using the Quant-it Picogreen dsDNA quantitation assay and by agarose gel electrophoresis. Libraries were constructed using Illumina TruSeq DNA Sample Prep standard protocol. Briefly, 5 ug of high molecular weight genomic DNA (gDNA) was fragmented by Covaris sonication device. Following sonication, DNA fragments were end-repaired and A-tailed. Adapters were then ligated via a 3' thymine overhang. Finally, ligated fragments were amplified by PCR (10 cycles). insert sizes were about 400-500 bp as evaluated in an Agilent DNA 1000 Analyzer Chip.
ENA first public
2016-10-27
ENA last update
2015-02-05
External Id
SAMEA3233722
INSDC center alias
Fondazione E. Mach, Via E. Mach 1, I-38010, San Michele all'Adige (TN)
INSDC center name
Fondazione E. Mach, Via E. Mach 1, I-38010, San Michele all'Adige (TN)
INSDC first public
2016-10-27T17:01:17Z
INSDC last update
2015-02-05T14:22:56Z
INSDC status
public
Submitter Id
E-SYBR-13_2:T0FM
broker name
ArrayExpress
compound
Fluconazole
dose
256 microgram per millilter
phenotype
3% mistranslation of CUG codons
sample name
E-SYBR-13_2:T0FM
strain
T0FM