Homo sapiens
Protocols: Patients undergoing radical prostatectomy for organ confined prostate cancer were consented to allow the use of prostate tissue for research via the Biorepository at the Norfolk and Norwich University Hospital (NNUH) under ethical approval granted from the University of East Anglia (FMHS 20122014-37) to comply with the principles expressed in the Declaration of Helsinki. Patients underwent endoscopic extraperitoneal radical prostatectomy. All procedures were conducted by a single surgeon. The prostate gland was removed from the abdominal cavity immediately after resection and rapidly biopsied after extraction to avoid ischaemic artefacts using a standard core biopsy instrument. The extracted whole prostatectomy specimen was placed on a surgical table or equivalent and the subsequent steps were taken to collect the biopsy samples: (1) the apex and base of the gland were identified, the prostate was then cut transversely (axial section) half way along the gland, (2) using the midline and the urethra as guides, biopsies were taken from the peripheral and transition zones avoiding obvious tumour sites where feasible, (3) the remaining prostate gland was sent for histological analysis to inform the malignant status of each core. One tissue core from each zone was placed in RNAlater and stored at -80 °C for downstream RNA sequencing. Illumina barcoded TruSeq RNA library construction of 18 libraries from 18 total RNA samples
ENA first public
2017-07-04
ENA last update
2016-10-03
External Id
SAMEA4476441
INSDC center alias
Institute of Food Research
INSDC center name
Institute of Food Research
INSDC first public
2017-07-04T17:02:21Z
INSDC last update
2016-10-03T16:00:07Z
INSDC status
public
Submitter Id
E-MTAB-5021:PZ_4
broker name
ArrayExpress
clinical information
morphologically normal tissue adjacent to tumour
common name
human
disease
prostate carcinoma
histology
benign
individual
4
organism part
prostate
sample name
E-MTAB-5021:PZ_4
sampling site
peripheral zone of prostate
sex
male