Homo sapiens
MYC/RunX2 trasngenic mice were infected with WT or MLV mutants. Splenic tissue was then isolated from the infected mice at the day of death. Virus recovered from the extracted tissue samples were then added to 293mCAT cells. Cells were cultured for at least 7 days before harvest for DNA isolation and MLV integration sites were obtained using linker-mediated PCR (LM-PCR). For K562 samples, K562 cells (5x105 cells) were prepared a day prior for infection in 6-well plates. For WT MLV, 500 ng of p30 was added to one well of K562 cells and for MLV IN TP-, 5000 ng was added. The plate was spinoculated at 1,500 g for 1 hr then incubated for 4 hrs at 37oC. Supernatant was removed and the cells were grown for 24 hrs. The cells were collected for genomic DNA extraction and MLV integration sites were obtained using LM-PCR.
isolate
K562 and 293mCAT cell lines
age
N/A
biomaterial_provider
N/A
sex
missing
tissue
N/A
cell_line
K562 and 293mCAT
BioSampleModel
Human