Mus musculus
Protocols: oral gavage of helicobacter hepaticus at 1E8 bacterium on days 0,1 and 2 intraperitoneal injection of anti-IL10 receptor antibody at days 0 and 7 For simultaneous DNA and RNA extraction, pellets were dissolved in 500 ul extraction buffer (200 mM NaCl, 20 mM EDTA, 4 M guanidine thiocyanate, 1 % (v/v) β-mercaptoethanol) and 210 ul 20 % (w/v) SDS and lysed with a bead beater twice for 2 minutes employing Lysing Matrix E tubes (MP Biomedicals). Samples were centrifuged at 8000 g for 5 minutes and the supernatant was diluted with 3 volumes of lysing buffer (ZR-Duet DNA/RNA Miniprep Kit, Zymo Research). DNA and RNA were further extracted employing the ZR-Duet DNA/RNA Miniprep Kit according to the manufacturer's instructions. Total RNA was depleted of ribosomal RNA using Epibio's universal bacteria ribo-zero kit, single version. Libraries were constructed using the NEBNext Ultra directional RNA Library Prep Kit (E7420L).
ENA-FIRST-PUBLIC
2015-05-22T17:38:32Z
ENA-LAST-UPDATE
2018-03-09T01:19:04Z
External Id
SAMEA3403124
INSDC center name
Kennedy Institute of Rheumatology, University of Oxford
INSDC first public
2015-05-22T17:38:32Z
INSDC last update
2018-03-09T01:19:04Z
INSDC status
public
Submitter Id
E-MTAB-3562:rna_stool-HhaIL10R-R2
age
8 week
biological replicate
2
broker name
ArrayExpress
common name
house mouse
disease
colitis
sample name
E-MTAB-3562:rna_stool-HhaIL10R-R2
scientific_name
Mus musculus
sex
male
starting material
Faeces
strain
C57BL/6
treatment
Helicobacter hepaticus gavage (10E8 bacteria) with Intraperitoneal injection of 10mg anti-IL10R antibody on days 0 and 7