Mus musculus
Protocols: mESCs were grown in Glasgow Minimum Essential medium supplemented with 20% FBS, 1% Glutamax, 1% Penicillin/Streptomyocin, 1% Non-Essential aminoacids, 1% Sodium Pyruvate and 0.2% _-mecaptoethanol. The medium was supplemented with LIF. The cells were maintained at sub confluent amounts and were grown at 37 degree celsius and 5% CO2. The knockdown was done with mouse SPOC1 (Phf13) or scrambled shRNA lenti-viruses purchased from Sigma (Mission NM_172705). Total cellular RNA was collected using RNeasy plus mini-kit (Qiagen) according to manufactures instructions. 1 microgram of total RNA was then depleted of ribosomal RNA using the Ribo zero magnetic kit (Epicenter) according to the manufacturer's directions. The rRNA depleted RNA was then measured by Qubit and 50ng was used to generate the RNA library using ScriptSeq v2 RNA-Seq library preparation kit (Epicenter) according to the manufacturer's instructions.
ENA-FIRST-PUBLIC
2016-05-31T17:01:37Z
ENA-LAST-UPDATE
2018-03-08T18:34:09Z
External Id
SAMEA2603465
INSDC center name
Max Planck Institute for Molecular Genetics, OWL Epigenomics
INSDC first public
2016-05-31T17:01:37Z
INSDC last update
2018-03-08T18:34:09Z
INSDC status
public
Submitter Id
E-MTAB-2637:RNA-seq PHF13 knockdown
broker name
ArrayExpress
cell line
ES-E14 cell
cell type
embryonic stem cell
common name
house mouse
sample name
E-MTAB-2637:RNA-seq PHF13 knockdown
scientific_name
Mus musculus