Homo sapiens
Protocols: Cells purchased from Lonza. They were thawed in February 2016 and cultured for approximately 12 days to reach a confluency of about 90%. Culture medium: BEBM (Lonza) plus human recombinant EGF, Hydrocortisone, Bovine Pituitary Extract, Epinephrine, Transferrin, Insulin, Triiodothyronine, and GA-1000. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced
ENA first public
2017-07-04
ENA last update
2017-07-04
External Id
SAMEA104151471
INSDC center alias
Glaxo Smithkline
INSDC center name
Glaxo Smithkline
INSDC first public
2017-07-04T17:02:27Z
INSDC last update
2017-07-04T10:29:42Z
INSDC status
public
Submitter Id
E-MTAB-47291499159733:NHBE_1_RNA
broker name
ArrayExpress
cell line
NHBE
cell type
epithelial cell
common name
human
disease
normal
organism part
epithelium of bronchus
sample name
E-MTAB-47291499159733:NHBE_1_RNA