control in high-fat-diet #2
Mus musculus
Protocols: After sacrifice, the blood was flushed from circulation by infusing pressurized phosphate buffered saline from the left ventricle of the animal. The entire aortae, which included the distal portion of the ascending aortae, aortic arches, and descending aortae down to the liac bifurcations, were excised from the sacrificed animals, under dissecting microscope. The excised aortae were thoroughly cleaned under dissection microscope to be free of other tissue such as adipose, muscle, and connective tissues. They were then stored in -80 degree until the extraction of RNA. After sacrifice, the blood was flushed from circulation by infusing pressurized phosphate buffered saline from the left ventricle of the animal. The entire aortae, which included the distal portion of the ascending aortae, aortic arches, and descending aortae down to the liac bifurcations, were excised from the sacrificed animals, under dissecting microscope. The excised aortae were thoroughly cleaned under dissection microscope to be free of other tissue such as adipose, muscle, and connective tissues. They were then stored in -80 degree until the extraction of RNA. All mice were placed on high-fat diet (HFD, 1.25% cholesterol, 40% kcal fat; D12108Ci, Research Diets, Inc., New Brunswick, NJ) at 8th week of age and maintained on the diet for 16 weeks. HFD contained either ticagrelor, clopidogrel, or nothing (control). The mice were fed HFD containing either ticagrelor (180mg/kg/day), clopidogrel (25 mg/kg/day), or nothing (control). The doses of ticagrelor and clopidogrel completely inhibited the platelet aggregation by light transmission aggregometry. Illumina software bcl2fastq v2.17.1.14 was used to convert bcl files into fastq format. Total RNA was isolated individually from each aorta using Qiagen RNA purification columns. The quality of the RNA was examined, using the Agilent 2100 Bioanalyzer and found that all samples had RNA integrity numbers higher than 8. RNA samples were polyA selected and paired-end sequencing libraries were constructed using TruSeq RNA Sample Prep Kit as described in the TruSeq RNA Sample Preparation V2 Guide (Illumina).
ENA-FIRST-PUBLIC
2019-06-16T04:04:29Z
ENA-LAST-UPDATE
2019-06-11T09:51:34Z
External Id
SAMEA5704565
INSDC center name
University of Texas Medical Branch at Galveston (UTMB)
INSDC first public
2019-06-16T04:04:29Z
INSDC last update
2019-06-11T09:51:34Z
INSDC status
public
Submitter Id
E-MTAB-8050:control in high-fat-diet #2
age
8
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
LDLR-/-APOBEC1-/-
organism part
aorta
sample name
E-MTAB-8050:control in high-fat-diet #2
scientific_name
Mus musculus
sex
male
strain
C57BL/6J