Caenorhabditis elegans CB4689 larval L4 male RNA biological replicate M-2_2 RNAseq
 CB4689_L4_M-2_2-MRZ RNAseq  Caenorhabditis elegans CB4689 larval L4 male RNA biological replicate M-2_2 RNAseq.   For the males we 1) grow dpy-28(y1);him-8(e1489) strain at 15 deg until majority of animals are gravid.  2) We bleach the worms to collect embryos, and then place the embryos in S-basal buffer an d incubate at 25 deg over night to hatch the L1s.  3) We then put the hatched L1s on a seeded NGM plate at 25 deg for 30 hours.  At 30 hours, the resulting worms are in the L4 stage, and all are males because the hermpahrodites die due to a failure to initiate dosage compensation, as confirmed by examination under the dissecting microscope.  4) At this time, the worms are harvested, placed into Trizol, and then subjected to three rounds of freeze/thaw, before being submitted for RNA isolation. L4 males L4_M-2 ribozero low  input treated total RNA.  Sample obtained from the lab of David M. Miller III, Vanderbilt University. Library production and sequencing in the lab of Robert H. Waterston, University of Washington School of Medicine.  Quality values are ASCII encoded Phred + 33.      