Knockdown 1
Drosophila melanogaster
Protocols: 60 wing imaginal discs per sample were dissected from wandering 3rd instar larvae. Nubbin-Gal4 homozygous females were crossed to homozygous UAS-Dis3RNAi males at 25 degrees C.All offspring were of knockdown (nubbin-Gal4/UAS-Dis3RNAi) genotype. RNA was extracted from each sample of 60 wing discs using a miRNeasy kit. RNA was size selected (18-22nt) then libraries were constructed using illumina TruSeq Small RNA sample preparation protocol.
ENA-FIRST-PUBLIC
2015-06-01T16:20:36Z
ENA-LAST-UPDATE
2018-03-09T01:12:28Z
External Id
SAMEA3400836
INSDC center name
Brighton and Sussex Medical School
INSDC first public
2015-06-01T16:20:36Z
INSDC last update
2018-03-09T01:12:28Z
INSDC status
public
Submitter Id
E-MTAB-3569:Knockdown 1
broker name
ArrayExpress
common name
fruit fly
developmental stage
third instar larva stage
phenotype
Knockdown
rna interference
Knockdown - contains both Nubbin-Gal4 and UAS-Dis3RNAi constructs
sample name
E-MTAB-3569:Knockdown 1
scientific_name
Drosophila melanogaster