PM3
Mus musculus domesticus
Protocols: Two batches of C57Bl6/j (WT) animals (10 each) were implanted with placebo or morphine pellets as mentioned above, for 24 or 48 hours and their fecal contents collected and pooled. The fecal content was processed according to fecal microbial transplant (FMT) procedure described for human patients52 with modifications. Briefly, the fecal contents were suspended in PBS (10mg/ml; w/v), filtered through a 40 mesh and centrifuged at 6,000Xg for 20 minutes. The resultant microbial pellet was resuspended in half volume of chilled PBS and aliquoted into volumes for single thaw and use. Aliquotes destined for later use were reconstituted to 10% sterile glycerol (Sigma) and stored at -80. A total of 32 WT mice were used for the transplant experiment. Animals were implanted with placebo or morphine pellets (12 each) and the stored microbiota was administered (106 CFUs/ dose) via oral gavage every 24 hours thrice according to the following scheme: [a] Placebo pellet implanted animals getting placebo microbiome (PP; n=8), [b] Placebo pellet implanted animals getting morphine microbiome (PM; n=8), [c] Morphine pellet implanted animals getting placebo microbiome (MP; n=8) and [d] Morphine pellet implanted animals getting morphine microbiome (MM; n=8). Animals were sacrificed 24hours after third transplant and fecal contents and tissues harvested for various downstream analyses. The fecal matter was lysed using glass beads in MagnaLyser tissue disruptor (Roche) and total DNA isolated using Power-soil/fecal DNA isolation kit (Mo-Bio) as per manufacturers specifications. V4-V5 consensus
ENA first public
2016-06-01
ENA last update
2015-12-10
External Id
SAMEA3707017
INSDC center alias
UMENT
INSDC center name
University of Minnesota
INSDC first public
2016-06-01T17:01:04Z
INSDC last update
2015-12-10T16:40:04Z
INSDC status
public
Submitter Id
E-MTAB-37232:PM3
broker name
ArrayExpress
common name
western European house mouse
environmental history
transplanted microbiome
organism part
feces
sample name
E-MTAB-37232:PM3