CD95LMUTNP overexpressed in HCT116 Drosha KO cells rep 2
Homo sapiens
source_name
ATCC CCL-247; Drosha KO cells provided by Narry Kim, ref: Kim Y.K., Kim B., Kim V.N. (2016). Re-evaluation of the roles of DROSHA, Export in 5, and DICER in microRNA biogenesis. Proc Natl Acad Sci U S A. 113(13):E1881-9.
genotype/variation
Drosha k.o.
cell line
HCT116
tissue
colon
treatment/infection
Cells infected with pLenti-CD95LMUTNP and selected with 3ug/mL puromycin for 2 days. Cells were plated for 50hours and an Ago-pull down was performed and the small RNA (19-23nts) sequenced.