PDAC021T
Homo sapiens
Protocols: two tumor sampling methods from patients with PDAC: 1- surgery for patients with tumor size < 2cm, and 2- Endoscopic Ultrasound-Guided Fine-Needle Aspiration (EUS-FNA) for patients with tumor size > 2cm. this information is given by the \Resectable\ variable. For operated patients (with surgery), tumor tissues were xenografted directly. However, biopsy tissues were grown in culture for 1 or 2 days, before transplanted to the nude mouse. The final xenograft samples were collected from mouse when tumors reached a size of 1 cm. The \Differentiation\, \Fibrosis\, \Inflammation\ information are estimated on the collected xenograft tissues. miRNA-Seq libraries are performed from at least 1ug of extracted total RNA with a RIN greater than 7. Before starting, total RNAs are purified with Trizol-GT & miRNeasy kit wich allows the selection of the small RNA fraction less than 100b.
ENA-FIRST-PUBLIC
2017-12-12T17:02:53Z
ENA-LAST-UPDATE
2017-03-07T13:50:18Z
External Id
SAMEA103893910
INSDC center name
Programme Carte dâ€™Identite des Tumeurs
INSDC first public
2017-12-12T17:02:53Z
INSDC last update
2017-03-07T13:50:18Z
INSDC status
public
ProportionMurineRNA
0.13
Submitter Id
E-MTAB-5018:PDAC021T
broker name
ArrayExpress
common name
human
disease
pancreatic ductal adenocarcinoma
individual
PDAC021
organism status
dead
resectable
true
sample name
E-MTAB-5018:PDAC021T
scientific_name
Homo sapiens
sex
female
survival time
6
xenograft
pancreatic cancer cells grown in nude mouse
xenograft CIMPclass
false
xenograft MultiOmicsClassification
basal
xenograft differentiation
non-mucinous
xenograft fibrosis
false
xenograft inflammation
false