25_14R_rapa_food_F_4th_24M_3M_NL
Mus musculus
fecal samples collected from the same cage of mice after rapamaycin feeding starting at 28 months old
ENA first public
2016-06-01
ENA last update
2016-10-21
External Id
SAMEA4016331
INSDC center alias
FRED HUTCHINSON CANCER RESEARCH CENTER
INSDC center name
FRED HUTCHINSON CANCER RESEARCH CENTER
INSDC first public
2016-06-01T17:01:04Z
INSDC last update
2016-10-21T09:40:14Z
INSDC status
public
Submitter Id
CHIT_25
amount or size of sample collected
0.4
chemical administration
feeding food containing equivalent amount of capsules and 0.3% agar for 3 months
collection date
2014-09
common name
house mouse
environment (biome)
Foege building
environment (feature)
Intestine environment(ENVO:2100002)
environment (material)
feces(ENVO:00002003)
geographic location (country and/or sea)
USA
geographic location (latitude)
156.216667
geographic location (longitude)
-172.583333
host age
31
host body habitat
colon
host common name
house mouse
host diet
Picolab Rodent Diet 20 #5053 (Lab Diet, St. Louis, MO) & 0.3 % agar
host genotype
C57BL6/JNia
host phenotype
old
host sex
female
host-associated environmental package
host-associated
investigation type
metagenome
nucleic acid amplification
PCR
nucleic acid extraction
isopropanol precipitation
pcr conditions
PCR reactions (50 uL) contained 100 ng of genomic DNA, forward and reverse primers (0.2 uM each), dNTPs (200 uM each), and Phusion High-Fidelity DNA Polymerase (1U). PCR amplification was performed as follows: amplification at 98�C for 3 minutes, and 25 cycles at 98�C for denaturation for 15 seconds, annealing at 50�C for 30 seconds, and extension at 72�C for 30 seconds, then a final extension at 72�C for 7 minutes.
pcr primers
Universal primers (U515F/806R)
project name
mouse gut microbiome change by rapamycin
sample name
CHIT_25
sample volume or weight for DNA extraction
0.4
sequencing method
Illumina MiSeq