CIPR 34.1.E
Mus musculus
Protocols: Where necessary, four to six week old mice were tail vein injected with AAV9 virus 1x10 11. At adulthood animals requiring 4-OHT were i.p. injected with 1 mg in 10 % ethanol and vegetable oil and collected 4. Mouse tissue was harvested from mice directly into liquid nitrogen. Total RNA was purified using PureLink RNA Mini Kit (Thermo Scientific) together with on column DNA digestion using PureLink DNase (Thermo Scientific). Then 1 to 5 µg of purified RNA was treated with Ribozero rRNA removal kit (Illumina) and ethanol precipitated. RNA quality and removal of rRNA were checked with the Agilent 2100 Bioanalyser (Agilent Technologies). Libraries for RNA-seq were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB) following manufacturers instructions.
ENA-FIRST-PUBLIC
2020-02-24T04:09:29Z
ENA-LAST-UPDATE
2019-11-18T10:48:26Z
External Id
SAMEA6232859
INSDC center name
UNIVERSITY OF CAMBRIDGE
INSDC first public
2020-02-24T04:09:29Z
INSDC last update
2019-11-18T10:48:26Z
INSDC status
public
Submitter Id
E-MTAB-8515:CIPR 34.1.E
age
8
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
AAV9-Ccnt1;LSL-R26CAG-c-MycERT2/+
individual
11
organism part
cardiomyocyte
sample name
E-MTAB-8515:CIPR 34.1.E
scientific_name
Mus musculus
sex
female