Sample 70
Homo sapiens
Protocols: Preserved and lesioned cartilage samples from the same donor were obtained from the RAAK study consisting of OA patients that underwent a joint replacement surgery due to end-stage disease.collection (within 2 hours following surgery) tissue was washed extensively with phosphate buffered saline (PBS) to decrease the risk of contamination by blood. Cartilage was classified macroscopically and collected separately from OA affected and preserved regions around the weight-bearing area of the joint. Total RNA from articular cartilage was isolated using Qiagen RNeasy Mini Kit (Qiagen, GmbH, Hilden, Germany). Paired-end 2×100 bp RNA library sequencing (Illumina TruSeq RNA Library Prep Kit) were constructed. Strand specific RNA-Seq libraries were generated and yielded a mean of 20 million reads per sample. Stranded TruSeq kit
ENA-FIRST-PUBLIC
2019-01-07T17:03:25Z
ENA-LAST-UPDATE
2018-11-08T17:04:57Z
External Id
SAMEA5075001
INSDC center name
Leiden University Medical Center-LUMC
INSDC first public
2019-01-07T17:03:25Z
INSDC last update
2018-11-08T17:04:57Z
INSDC status
public
Submitter Id
E-MTAB-7313:Sample 70
age
64
broker name
ArrayExpress
common name
human
disease
osteoarthritis
disease staging
preserved osteoarthritis
individual
Patient35
organism part
cartilage
sample name
E-MTAB-7313:Sample 70
sampling site
knee
scientific_name
Homo sapiens
sex
male