Sample 9
Homo sapiens
Protocols: Fragmentation of 100 ng purified genomic DNA was done on Covaris S2 and the procedure adjusted to obtain fragments with a peak length of 130 and 170 bp. Fragmentation of 100 ng purified genomic DNA (gDNA) in 55 μl Tris-EDTA buffer was done on Covaris S2 and the procedure adjusted to obtain fragments with a peak length of 130 and 170 bp, respectively. After library preparation from 100 ng of fragmented gDNA using Agilent SureSelectXT v5 (50 Mb) and v5+UTR (75 Mb), libraries were purified, size validated and prepared for sequencing according to the manufacturer's protocols. Libraries were sequenced on Illumina HiSeq2500 using TruSeq SBS Kit v3-HS (2x101, paired-end run).
DNA insert size
170
ENA first public
2016-06-01
ENA last update
2016-03-04
External Id
SAMEA3888325
INSDC center alias
Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
INSDC center name
Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
INSDC first public
2016-06-01T17:01:04Z
INSDC last update
2016-03-04T10:48:37Z
INSDC status
public
Submitter Id
E-MTAB-4527:Sample 9
broker name
ArrayExpress
cell line
NC-NC
common name
human
sample name
E-MTAB-4527:Sample 9