Mouse_Naive_2
Mus musculus
Protocols: T cells from blood (human) and mouse (spleen) Mononuclear cells were isolated from the cord blood of healthy neonates at Turku University Central Hospital using Ficoll-Paque PLUS (GE Healthcare, #17-1440-02). CD4+ T cells were then isolated using the Dynal bead-based positive isolation kit (Invitrogen). CD4+ cells from three individual donors were activated directly in 24w plates with plate-bound anti-CD3 (500ng/well, Immunotech) and soluble anti-CD28 (500 ng/mL, Immunotech) at a density of 2×106 cells/mL of Yssel's medium67 containing 1% human AB serum (PAA). Th2 cell polarization was initiated with IL-4 (10 ng/ml, R&D Systems). Cells activated without IL-4 were also cultured (Th0). Total RNA was purified by Qiagen RNeasy Mini Kit according to manufacturer's instruction, and concentration was determined by a Nanodrop. A total of 500 ng RNA was used to prepare sequencing libraries using KAPA Stranded mRNA-seq Kit (KAPA #07962193001) according to manufacturer's instructions.
ENA first public
2017-12-12
ENA last update
2017-12-12
External Id
SAMEA104423656
INSDC center alias
Sanger Institute
INSDC center name
Sanger Institute
INSDC first public
2017-12-12T17:01:54Z
INSDC last update
2017-12-12T11:42:19Z
INSDC status
public
Submitter Id
E-MTAB-6300:Mouse_Naive_2
age
6 to 8
broker name
ArrayExpress
cell type
naive T cell
common name
house mouse
disease
normal
genotype
wild type genotype
individual
donor pool 2
organism part
spleen
sample name
E-MTAB-6300:Mouse_Naive_2
strain
C57BL/6J