AB2.2 ESC total polyA
Mus musculus
Protocols: ESC colonies were maintained on gelatinized cell culture dishes, on a feeder layer of irradiated mouse embryonic fibroblasts in knock-out DMEM supplemented with 15 % fetal bovine serum, non-essential amino acids and 1,000 U/ml leukemia inhibiting factor. NPCs were maintained on gelatinized cell culture dishes in N2 expansion medium composed of 50:50 DMEM / F12: neurobasal medium, supplemented with 1x N2, 0.05x B-27, 50 ug /ml BSA (fraction V), 1ug / ml laminin, and 10 ng / ml each murine basic fibroblast growth factor and epidermal growth factor Total RNA was isolated using TRIzol reagent. For ASO knock-down studies, total RNA was collected using TRIzol and precipitated in the presence of 7.5 ug GlycoBlue (Ambion, Life Technologies). RNA integrity was assessed on the Agilent 2100 Bioanalyzer instrument and only RNA samples with integrity scores > 9.5 (median 10.0) were used. Stranded libraries for paired-end sequencing were prepared as described in Parkhomchuk, D., Borodina, T., Amstislavskiy, V., Banaru, M., Hallen, L., Krobitsch, S., Lehrach, H., and Soldatov, A. (2009). Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic Acids Res. 37, e123.
ENA-FIRST-PUBLIC
2015-06-01T16:20:35Z
ENA-LAST-UPDATE
2018-03-08T22:59:06Z
External Id
SAMEA3180061
INSDC center name
CSHL
INSDC first public
2015-06-01T16:20:35Z
INSDC last update
2018-03-08T22:59:06Z
INSDC status
public
Submitter Id
E-MTAB-3198:AB2.2 ESC total polyA
broker name
ArrayExpress
cell line
AB2.2
cell type
embryonic stem cell
cellular component
whole cell
common name
house mouse
sample name
E-MTAB-3198:AB2.2 ESC total polyA
scientific_name
Mus musculus
sex
male