CTL 1
Mus musculus
Protocols: Testes from adult mice were dissected, decapsulated and digested with collagenase and trypsin under gentle agitation at 32°C followed by pipetting to obtain a single-cell suspension. Cells were then stained with Hoechst 33342 and propidium iodide (PI). Pachytene-diplotene cells were FACS sorted according to their characteristic blue/red Hoechst fluorescence profile and GFP-high expression. Total RNA was extracted using QIAzol lysis reagent (Qiagen) according to manufacturer's instructions. Libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina following manufacturer's instructions
ENA-FIRST-PUBLIC
2019-01-14T17:03:31Z
ENA-LAST-UPDATE
2018-11-05T12:20:45Z
External Id
SAMEA5065520
INSDC center name
MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh
INSDC first public
2019-01-14T17:03:31Z
INSDC last update
2018-11-05T12:20:45Z
INSDC status
public
Submitter Id
E-MTAB-7065:CTL 1
broker name
ArrayExpress
cell cycle stage
pachytene-diplotene
cell type
spermatogonium
common name
house mouse
developmental stage
adult
genotype
Ythdf2 +/GFP-Fl
organism part
testis
sample name
E-MTAB-7065:CTL 1
scientific_name
Mus musculus
sex
male
strain
C57BL/6