Sample 1
Mus musculus
Protocols: Experiments were carried out in agreement with European Community Council Directive (86/609/EEC) and the animal ethics guidelines of the i3S, and approved by the Portuguese Veterinary Ethics Committee. Dorsal spinal cord tissues were collected from E14.5 embryos and then dissected in ice-cold PBS. Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011; Regadas et al, 2014), with an anti-Tlx3 antibody DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol.
ENA-FIRST-PUBLIC
2019-06-14T04:02:39Z
ENA-LAST-UPDATE
2018-07-04T15:12:48Z
External Id
SAMEA4776692
INSDC center name
Instituto Gulbenkian de Ciencia
INSDC first public
2019-06-14T04:02:39Z
INSDC last update
2018-07-04T15:12:48Z
INSDC status
public
Submitter Id
E-MTAB-6974:Sample 1
broker name
ArrayExpress
cell type
neuron
common name
house mouse
developmental stage
embryonic day 14.5
organism part
dorsal spinal cord
sample name
E-MTAB-6974:Sample 1
scientific_name
Mus musculus