ROMEO2015.001.DNA_2015-04-07_Miss_A_spring_river_water
freshwater metagenome
USGS 07374525 Mississippi River at Belle Chasse, LA
isolation_source
river water
collection_date
2015-04-07
geo_loc_name
USA: Mississippi River at Belle Chasse, LA
lat_lon
29.857150 N 89.977850 W
samp_collect_device
Collected into a 10L carboy dipped below the surface near the center of the river channel
samp_mat_process
Material pre-filtered with a 3.0 micron pore-size, 142mm diameter polycarbonate filter, and collected on a 0.22 micron pore-size Sterivex filter (Millipore, Burlington, MA). Genomic DNA from Thermus thermophilus HB8 was added (44 ng) prior extraction for quantification analyses, as per Satinsky et al. 2013 (https://doi.org/10.1016/B978-0-12-407863-5.00012-5). DNA was extracted using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI).
samp_size
1.18 L
isolate
Miss_A_spring
replicate
replicate A
BioSampleModel
Metagenome or environmental