Mersitems_B_lane1
Solanum lycopersicum
Protocols: Samples were harvested at the end of the day, and consisted of pools of at least a hundred whole shoot apical meristems or at least six first leaves. Samples were collected from two week old plants of tomato. S. lycopersicum seeds were directly sown in soil (Levington F2, ICL) and stratified in the dark for one week at 4°C. Plants were grown in a Conviron MTPS chamber (Conviron) at 25/18°C (day/night); with 16/8 hours light cycle, 300 µmol/m2/s light intensity and ~60% humidity. DNA was extracted with the DNeasy Plant Mini Kit (Qiagen). PCR-free libraries for the DNA-seq were prepared with 1 ug of DNA using the TruSeq DNA PCR-Free Library Prep kit (Illumina); following provider's instructions.
ENA-FIRST-PUBLIC
2019-06-14T04:02:46Z
ENA-LAST-UPDATE
2019-03-29T14:10:02Z
External Id
SAMEA5542010
INSDC center name
IFEVA (CONICET-UBA), Facultad de Agronomia (FAUBA),Universidad de Buenos Aires (UBA)
INSDC first public
2019-06-14T04:02:46Z
INSDC last update
2019-03-29T14:10:02Z
INSDC status
public
Submitter Id
E-MTAB-7823:Mersitems_B_lane1
age
15 days old
broker name
ArrayExpress
common name
tomato
cultivar
M82
developmental stage
vegetative
genotype
wild-type
organism part
shoot apical meristem
sample name
E-MTAB-7823:Mersitems_B_lane1
scientific_name
Solanum lycopersicum