clopidogrel in high-fat-diet #3
Mus musculus
Protocols: After sacrifice, the blood was flushed from circulation by infusing pressurized phosphate buffered saline from the left ventricle of the animal. The entire liver were excised from the sacrificed animals, under dissecting microscope. The excised liver were thoroughly cleaned under dissection microscope to be free of other tissue such as adipose, muscle, and connective tissues. They were then stored in -80 degree until the extraction of RNA. After sacrifice, the blood was flushed from circulation by infusing pressurized phosphate buffered saline from the left ventricle of the animal. The entire liver was excised from the sacrificed animals, under dissecting microscope. The excised liver was thoroughly cleaned under dissection microscope to be free of other tissue such as adipose, muscle, and connective tissues. They were then stored in -80 degree until the extraction of RNA. All mice were placed on high-fat diet (HFD, 1.25% cholesterol, 40% kcal fat; D12108Ci, Research Diets, Inc., New Brunswick, NJ) at 8th week of age and maintained on the diet for 16 weeks. HFD contained either ticagrelor, clopidogrel, or nothing (control). The mice were fed HFD containing either ticagrelor (180mg/kg/day), clopidogrel (25 mg/kg/day), or nothing (control). The doses of ticagrelor and clopidogrel completely inhibited the platelet aggregation by light transmission aggregometry. Illumina software bcl2fastq v2.17.1.14 was used to convert bcl files into fastq format. Total RNA was isolated individually from each aorta using Qiagen RNA purification columns. The quality of the RNA was examined, using the Agilent 2100 Bioanalyzer and found that all samples had RNA integrity numbers higher than 8. RNA samples were polyA selected and paired-end sequencing libraries were constructed using TruSeq RNA Sample Prep Kit as described in the TruSeq RNA Sample Preparation V2 Guide (Illumina).
ENA-FIRST-PUBLIC
2019-06-16T04:04:29Z
ENA-LAST-UPDATE
2019-06-11T09:51:48Z
External Id
SAMEA5704576
INSDC center name
University of Texas Medical Branch at Galveston (UTMB)
INSDC first public
2019-06-16T04:04:29Z
INSDC last update
2019-06-11T09:51:48Z
INSDC status
public
Submitter Id
E-MTAB-8049:clopidogrel in high-fat-diet #3
age
8
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
Ldlr-/-Apobec1-/-
individual
X27
organism part
liver
sample name
E-MTAB-8049:clopidogrel in high-fat-diet #3
scientific_name
Mus musculus
sex
male
strain
C57BL/6J