Sample 8
Mus musculus
Protocols: wild-type mice and Nkx3.2-Cre;Tcf7l2 f/f mice were generated and their pancreatic tissues dissected at 10 weeks of age For cell isolation, dissected pancreatic tissues were digested with Collagenase P (0.4 mg/ml; Roche) and DNase (0.1 ng/ml; Sigma) diluted in HBSS for 30 minutes at 37 ̊C to obtain single cell. DAPI (1 l/ml; Sigma-Aldrich) was added to label dead cells. Live fluorescently-labeled cells were collected using FACS Aria (BD). Using PureLink RNA micro kit RNA was amplified using SMART-Seq v4 Ultra low Input RNA kit for Sequencing (Clontech), followed by Illumina sequencing library preparation
ENA first public
2017-12-14
ENA last update
2017-02-02
External Id
SAMEA72884668
INSDC center alias
Dept. of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
INSDC center name
Dept. of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
INSDC first public
2017-12-14T17:03:03Z
INSDC last update
2017-02-02T10:48:38Z
INSDC status
public
Submitter Id
E-MTAB-5325:Sample 8
broker name
ArrayExpress
cell type
endocrine pancreas
common name
house mouse
genotype
Nkx3.2-Cre;EYFP
organism part
islet of Langerhans
sample name
E-MTAB-5325:Sample 8
strain
C57BL/6