Acanthamoeba castellanii strain Neff (ATCC-30010) - monoclonal isolate of a mutant (pGAPDH-EGFP)
Acanthamoeba castellanii
This sample was used for a sequence-based exploration of transgene integration in Acanthamoeba castellanii. Acanthamoeba castellanii strain Neff (ATCC-30010) cells were transformed with plasmid pGAPDH-EGFP and maintained in PYG medium under selection with G418. Clones were isolated by allowing cells to disperse on agar plates and picking individual cells to grow in PYG medium. One such clone was used for this experiment. Genomic DNA from the cultures grown from these this transformed clone was sequenced with the Oxford Nanopore MinION.
strain
Neff (ATCC-30010)
collection_date
1957
env_broad_scale
N/A
env_local_scale
N/A
env_medium
Soil
geo_loc_name
USA: Pacific Grove, California
isol_growth_condt
Axenic growth in PYG-like medium
lat_lon
36.62 N 121.92 W
biotic_relationship
free living
estimated_size
About 42 000 000 bp
isolation_source
soil
ploidy
unknown
propagation
eukaryote: unknown sexuality
BioSampleModel
MIGS.eu