H3K27me3_ICeChIP-seq_S2_ChIP_P1000
Drosophila melanogaster
source_name
Cell culture
cell line
S2
chip antibody
H3K27me3 (Millipore, 07-449, lot 2275589)
growth protocol
S2 cells were grown in 24°C, 95% humidity in Shneider S2 Drosophila medium(Gibco) supplemented with 1X Insect Media Supplement(Sigma) and 1X Penicillin/Streptomycin (Gibco). S2 cultures were grown in T-175 polystyrene dishes, passaged 1:5 every 5 days, Cells used in ChIP experiments were harvested when 80-90% confluent.
amplification cycles
10
map quality cut-off
20
mononucleosome cut-off
190
reference genome
dm3