Sample 6
Mus musculus
Protocols: C57BL/6J mice were purchased at 8 weeks old, acclimatized for one week, and sham-operated at 9 weeks old. After 45 days of recovery at basal laboratory conditions (chow at libitum, 22C), mice were fasted for 4 hours in the morning. Subsequently, mice were euthanized by cardiac puncture under isoflurane anesthesia, and the interscapular BAT depots were obtained. Total RNA was isolated using the TriPure isolation reagent (Roche Diagnostics, Mannheim, Germany) and contaminating genomic DNA was removed by the RQ1 RNase-free DNase (Promega Benelux, Leiden, Netherlands), according to the manufacturers' instructions. RNA quality was assessed by an Agilent 2100 Bioanalyzer (Agilent Technologies, Amstelveen, Netherlands). A TruSeq RNA Library Prep Kit v2 (Illumina, Eindhoven, Netherlands) was used for library preparation according to the manufacturer's instructions.
ENA-FIRST-PUBLIC
2021-01-16T04:08:47Z
ENA-LAST-UPDATE
2020-01-22T14:28:20Z
External Id
SAMEA6497490
INSDC center name
Dept. of Internal Medicine Erasmus MC
INSDC first public
2021-01-16T04:08:47Z
INSDC last update
2020-01-22T14:28:20Z
INSDC status
public
Submitter Id
E-MTAB-8717:Sample 6
age
16
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
F2
organism part
brown adipose tissue
sample name
E-MTAB-8717:Sample 6
sampling site
interscapular fat pad
scientific_name
Mus musculus
sex
female
strain
C57BL/6J