R3_0mM_24h
Arabidopsis thaliana
Protocols: 3 hours and 24 hours after transfer, seedlings were harvested in liquid nitrogen, put in RNAlater ice solution and incubated at -20°C for at least one week, after which the third leaf was micro-dissected under a binocular light microscope. Seedlings are grown on a mesh covering MS media without carbon source, and subsequently transferred at 9 DAS to control media without sucrose or medium supplemented with 15 mM sucrose. Transfer at 9 DAS to control media without sucrose or medium supplemented with 15 mM sucrose Leaves were frozen in liquid nitrogen and RNA was extracted using Trizol (Invitrogen) and the RNeasy Plant Mini Kit (Qiagen). Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). Briefly, polyA-containing mRNA molecules were reverse transcribed, double-stranded cDNA was generated and adapters were ligated. After quality control using 2100 Bioanalyzer (Agilent), clusters were generated through amplification using the TruSeq PE Cluster Kit v3-cBot-HS kit (Illumina).
ENA first public
2017-12-14
ENA last update
2016-11-17
External Id
SAMEA4553575
INSDC center alias
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
INSDC center name
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
INSDC first public
2017-12-14T17:03:01Z
INSDC last update
2016-11-17T14:57:46Z
INSDC status
public
Submitter Id
E-MTAB-4262_2:R3_0mM_24h
broker name
ArrayExpress
common name
thale cress
ecotype
Col-0
organism part
Leaf 3
sample name
E-MTAB-4262_2:R3_0mM_24h
time
24