5635sTS.Human.Hindbrain.5w.Female
Homo sapiens
Protocols: The organs were dissected from 23 developmental stages (starting at 4 weeks post conception until 58-63 years of age). The prenatal brain was divided into two regions: forebrain together with the midbrain (referred to as the 'brain') and hindbrain (referred to as the 'cerebellum'). The postnatal 'brain' and 'cerebellum' samples comprise the dorsolateral prefrontal region of the cerebral cortex and lateral cerebellar cortex, respectively. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).
ENA-FIRST-PUBLIC
2018-12-30T17:01:52Z
ENA-LAST-UPDATE
2018-05-25T13:50:11Z
External Id
SAMEA4690107
INSDC center name
University of Heidelberg
INSDC first public
2018-12-30T17:01:52Z
INSDC last update
2018-05-25T13:50:11Z
INSDC status
public
Submitter Id
E-MTAB-6814:5635sTS.Human.Hindbrain.5w.Female
age
5
broker name
ArrayExpress
common name
human
developmental stage
5 week post conception
developmental stage
embryo
disease
normal
organism part
hindbrain
sample name
E-MTAB-6814:5635sTS.Human.Hindbrain.5w.Female
scientific_name
Homo sapiens
sex
female