BAG6KO3
Mus musculus
Protocols: EVs were collected in cell culture for 48h in protein-free CD293 medium and isolated by (ultra-) centrifugation. The (ultra-)centrifugation protocol included consecutive pre-centrifugation steps at 300 x g (10 min), 2,000 x g (10 min) and 3,500 x g (20 min) for clearance of cells and cellular debris before ultracentrifugation at 100,000 x g (90 min) using rotor Type 45Ti. EVs were washed two times by resuspension in PBS, with the final run using TLA-45 rotor. B-16V (CVCL_1950)cells were maintained according to the DSMZ guidelines in RPMI (Gibco, USA), supplemented with 0.5% penicillin-streptomycin (Gibco, USA), and 10% fetal bovine serum (Gibco, USA). Cells were kept under standard culturing conditions with 5% CO2, 37 degrees centigrade. EV pellet was resuspended in lysis buffer and RNA was isolated using the RNeasy Mini Kit according to the manufacturer's protocol recommended for processing animal cell lysates. TruSeq Stranded mRNA Library Preparation Kit
ENA-FIRST-PUBLIC
2019-01-01T17:01:59Z
ENA-LAST-UPDATE
2018-08-06T16:56:41Z
External Id
SAMEA4819957
INSDC center name
Center for Immune and Tumor Biology
INSDC first public
2019-01-01T17:01:59Z
INSDC last update
2018-08-06T16:56:41Z
INSDC status
public
Submitter Id
E-MTAB-7123:BAG6KO3
broker name
ArrayExpress
cell line
B16V
cell type
epithelial cell
common name
house mouse
disease
melanoma
genotype
BAG6 homozygous knockout
organism part
skin
sample name
E-MTAB-7123:BAG6KO3
scientific_name
Mus musculus