unsorted fraction
Saccharomyces cerevisiae
Transformed library unsorted fraction
strain
ROY5634 (library)
isolation_source
ROY5634
collection_date
not collected
geo_loc_name
not applicable
sample_type
cell culture
genotype
MATa ADE2 lys2D leu2-3,112 his3-11 ura3-1 trp1-1 can1-100 rpl18b::BFP2-2
dev_stage
OD600 described in growth protocol
tissue
liquid culture
growth_protocol
5ml of yeast cells containing the library were used to inoculate 50ml YMD medium containing adenine, leucine, lysine, histidine and tryptophan (lacking uracil) and cells were grown at room temperature overnight (approximately 3 doublings). This culture was then used to inoculate 250ml YMD-uracil and grown at 30C for 3h. Cells were pelleted and resuspended into 250ul 1xPBS and 1%BSA at a concentration of 1 OD/ml, filtered through a Nitex mesh and sorted.
treatment
unsorted
BioSampleModel
Microbe, viral or environmental