RE-16aug2020-ssRNA
freshwater metagenome
Strand-specific freshwater metatranscriptome RE-16aug2020-ssRNA Sampling was performed on the 16th of August 2020 at 9:00 in Rimov reservoir 0.5m, Czech Republic, (48°50'54.4N 14°29'16.7E) using a hand-held vertical Friedinger (2 L) sampler. A total of 20 L of water were collected from a depth of 0.5m and immediately transported to the laboratory. Sample was subjected to rRNA depletion and used for stranded library preparation. Strand-specificity was achieved by incorporation of dUTPs instead of dTTPs in the second-strand cDNA followed by digestion of dUTPs by uracil-DNA glycosylase to prevent PCR amplification of this strand. Paired-end (PE 150-bp) sequencing was carried out using a Novaseq 6000 platform.
ENA first public
2021-01-14
ENA last update
2020-11-25
External Id
SAMEA7625098
INSDC center alias
BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO
INSDC center name
BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO
INSDC first public
2021-01-14T17:19:15Z
INSDC last update
2020-11-25T15:13:56Z
INSDC status
public
Submitter Id
RE-16aug2020-ssRNA
sample name
RE-16aug2020-ssRNA