Sample 13
Mus musculus
Protocols: Tumor burden was evaluated by quantification of visible tumors at different regions of the gut and measurement of tumors size was done using a caliper (Fine Science Tools). Intestine, liver, spleen, kidney and lung were subjected to fixation in 4% formaldehyde and paraffin embedding (FFPE). Duodenum, jejunum, ileum and colon were flushed with ice-cold PBS and cut open longitudinally prior the fixation. Intestinal epithelial cells from different gut sections were isolated as described in Elyada, E. et al., Nature 470, 409-413. Mice carrying a heterozygous germline nonsense mutation at codon 850 of Apc (Apcmin/+) were purchased from Jackson Laboratory (C57BL/6J-ApcMin/J) (Ref) and Apcmin/+/p53R172H mice were generated by crossing mice carrying knock-in Trp53 R172H mutation with Apcmin/+ mice. DNA was extracted (DNeasy Blood and Tissue kit, QIAGEN) from tumors and adjacent tissues isolated from Apcmin/+ mice. For Trp53 sequencing, the DNA binding domain-coding exons 5 to 8 were PCR amplified. PCR products were cleaned from primers using AMPure XP beads (Illumina). Samples were barcoded, cleaned with AMPure XP beads and sequenced.
ENA-FIRST-PUBLIC
2020-02-25T04:08:19Z
ENA-LAST-UPDATE
2019-09-20T15:30:02Z
External Id
SAMEA5790015
INSDC center name
Institute of Molecular Oncology, Center for Tumor and Immunobiology, Philipps-University Marburg
INSDC first public
2020-02-25T04:08:19Z
INSDC last update
2019-09-20T15:30:02Z
INSDC status
public
Submitter Id
E-MTAB-8355:Sample 13
age
3 to 5
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
Apc min/+ p53 R172H/+
organism part
colon
sample name
E-MTAB-8355:Sample 13
sampling site
neoplasm
scientific_name
Mus musculus
sex
female
strain
C57BL/6