K_2
Mus musculus
Protocols: Crypts were isolated from the small intestines of Bach2 KO and control mice (n=3, respectively) 10 days after tamoxifen administration. Total RNA was extracted for sequencing using RNA-Seq.RNA degradation and contamination was monitored on 1% agarose gels. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA).A total amount of 3ug RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® (NEB, USA) following manufacture's recommendations and index codes were added to attribute sequencing to each sample.
ENA first public
2021-01-12
ENA last update
2021-01-08
External Id
SAMEA7773326
INSDC center alias
FUDAN UNIVERSITY
INSDC center name
FUDAN UNIVERSITY
INSDC first public
2021-01-12T05:00:13Z
INSDC last update
2021-01-08T11:03:56Z
INSDC status
public
Submitter Id
E-MTAB-9865_2:K_2
age
12
broker name
ArrayExpress
cell type
intestinal epithelial cell
common name
house mouse
developmental stage
adult
genotype
Bach2-/-
organism part
epithelium of small intestine
sample name
E-MTAB-9865_2:K_2
sex
male
strain
C57BL/6J