TPseq_soma_3
Mus musculus
Protocols: Mouse embryonic stem cells with doxycycline-inducible ASCL1 cassette (ASCL1-mESC) were differentiated into neurons and separated on neurites and soma as previously described (Zappulo et al.RNA localisation is a key determinant of neurite-enriched proteome. Nature Comm. 2017). RNA was extracted with TRIFast reagent (Peqlab). 3' mRNA-seq was performed with QuantSeq 3' mRNA-Seq kit (Lexogen 015) according to the manufacturer's recommendations.
ENA-FIRST-PUBLIC
2019-01-12T17:03:23Z
ENA-LAST-UPDATE
2018-10-01T10:36:00Z
External Id
SAMEA4947849
INSDC center name
Max Delbrueck Center
INSDC first public
2019-01-12T17:03:23Z
INSDC last update
2018-10-01T10:36:00Z
INSDC status
public
Submitter Id
E-MTAB-7251:TPseq_soma_3
broker name
ArrayExpress
cell type
neuron
common name
house mouse
fraction
soma
genotype
doxycycline-inducible ASCL1 expression
progenitor cell type
embryonic stem cell
sample name
E-MTAB-7251:TPseq_soma_3
scientific_name
Mus musculus