Caenorhabditis elegans N2 early embryo
 EM_60-600_set1_RZ_SPIA RNAseq   Caenorhabditis elegans N2 early embryo, eggs harvested after 60 hours after L1 and incubated for 600 minutes.   Make eggs hatch in the absence of food to get them all in L1 arrest add food and wait 60 hours.  Eggs are then harvested from the adults -- just a few have an egg or two.  Then the eggs are incubated for 600 minutes. Library was spia amplified and ribo-zero treated.  Sample obtained from the lab of David M. Miller III, Vanderbilt University. Library production and sequencing in the lab of Robert H. Waterston, University of Washington School of Medicine.  Quality values are ASCII encoded Phred + 33.      