2756sTS.Rat.Kidney.2wpb.Female
Rattus norvegicus
Protocols: The organs were dissected from 16 developmental stages (starting at e11 until P112). Until e13 the dissected 'brain' samples correspond to the whole brain, and from e14 onwards they correspond to the cerebral hemispheres (without the olfactory bulbs). Early 'cerebellum' samples correspond to the prepontine hindbrain-enriched brain region (until ~ e18) and from then onwards only to the cerebellum. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).
ENA-FIRST-PUBLIC
2018-12-30T17:01:51Z
ENA-LAST-UPDATE
2018-05-23T11:12:38Z
External Id
SAMEA4680864
INSDC center name
The University of Heidelberg
INSDC first public
2018-12-30T17:01:51Z
INSDC last update
2018-05-23T11:12:38Z
INSDC status
public
Submitter Id
E-MTAB-6811:2756sTS.Rat.Kidney.2wpb.Female
age
14
broker name
ArrayExpress
common name
Norway rat
developmental stage
postnatal
genotype
wild type genotype
organism part
kidney
sample name
E-MTAB-6811:2756sTS.Rat.Kidney.2wpb.Female
scientific_name
Rattus norvegicus
sex
female
strain
Holtzman SD