ROMEO2016.008.RNA_2016-02-12_StLaw_B_winter_river_water
freshwater metagenome
USGS 04264331 St. Lawrence River at Cornwall, Ontario, near Massena, NY
isolation_source
river water
collection_date
2016-02-12
geo_loc_name
USA: St. Lawrence River at Cornwall, Ontario, near Massena, NY
lat_lon
45.000200 N 74.798674 W
samp_collect_device
Collected into a 10L carboy dipped below the surface near the right bank of the river channel
samp_mat_process
Material pre-filtered with a 3.0 micron pore-size, 142mm diameter polycarbonate filter, and collected on a 0.22 micron pore-size Sterivex filter (Millipore, Burlington, MA). Artificial transcript sequences (https://www.addgene.org; products MTST5 and MTST6) were added (0.65 ng of each) prior to nucleic acid extraction for quantification analyses, as per Satinsky et al. 2013 (https://doi.org/10.1016/B978-0-12-407863-5.00012-5). Following RNA extraction using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI), mRNA was enriched using Ribo-Zero microbial rRNA removal kit (Illumina, Inc.)
samp_size
5 L
isolate
StLaw_B_winter
replicate
replicate B
BioSampleModel
Metagenome or environmental