WT_PGA_stat_2
Dickeya dadantii
Protocols: Cells were collected in exponential phase (OD=0.2 at 600nm) or transition to stationary phase (OD=1.5 at 600nm). Growth in minimal medium M63 supplemented with sucrose (0.2% wt/vol), and, for 4 samples, with 0.2% PGA (polygalacturonate) Total RNA from each D. dadantii sample was isolated using the frozen acid phenol method (Maes et Messens 1992), and submitted to Vertis Biotechnologie AG for ribosomal depletion (Illumina RiboZero kit) and RNA fragmentation. Strand-specific cDNA libraries were constructed by Vertis Biotechnologie AG.
ENA-FIRST-PUBLIC
2019-04-16T17:03:56Z
ENA-LAST-UPDATE
2019-02-01T16:37:09Z
External Id
SAMEA5315652
INSDC center name
INSA Lyon
INSDC first public
2019-04-16T17:03:56Z
INSDC last update
2019-02-01T16:37:09Z
INSDC status
public
Submitter Id
E-MTAB-7650:WT_PGA_stat_2
broker name
ArrayExpress
growth condition
M63, sucrose, PGA
growth phase
transition to stationary
sample name
E-MTAB-7650:WT_PGA_stat_2
scientific_name
Dickeya dadantii
strain
3937