ROMEO2015.019.DNA_2015-07-21_StLaw_A_summer_river_water
freshwater metagenome
USGS 04264331 St. Lawrence River at Cornwall, Ontario, near Massena, NY
isolation_source
river water
collection_date
2015-07-21
geo_loc_name
USA: St. Lawrence River at Cornwall, Ontario, near Massena, NY
lat_lon
31.427170 N 81.605390 W
samp_collect_device
Collected into a 10L carboy dipped below the surface at the middle of the river channel
samp_mat_process
Material pre-filtered with a 3.0 micron pore-size, 142mm diameter polycarbonate filter, and collected on a 0.22 micron pore-size Sterivex filter (Millipore, Burlington, MA). Genomic DNA from Thermus thermophilus HB8 was added (44 ng) prior extraction for quantification analyses, as per Satinsky et al. 2013 (https://doi.org/10.1016/B978-0-12-407863-5.00012-5). DNA was extracted using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI).
samp_size
5 L
isolate
StLaw_A_summer
replicate
replicate A
BioSampleModel
Metagenome or environmental