Caenorhabditis elegans N2 larval L4 RNA biological replicate L4-2
 N2_L4-2-RZ RNAseq   Caenorhabditis elegans N2 larval L4 RNA biological replicate L4-2.  Animals were synchronized by treating a mixed population with bleach to collect embryos.  The embryos were incubated without food, causing the larvae to arrest at the L1 stage upon hatching.  Starved L1 larvae were plated in the presence of food and raised at 25 degrees Celsius. Staging was determined by examining a subset of animals under Nomarski optics at intervals for vulval and germline development.  Animals were collected at mid-L4 stage and washed in S basal buffer.  Exact times for growth after plating of starved L1s (all at 25 degrees C) were as follows:  L4 for 34.25 hours (vulvae are in Christmas tree stage gonad  has passed bend, sperm are present).   N2_L4-2 treated with ribozero low input total RNA.  Sample obtained from the lab of David M. Miller III, Vanderbilt University. Library production and sequencing in the lab of Robert H. Waterston, University of Washington School of Medicine.  Quality values are ASCII encoded Phred + 33.      