JEKO-1-PI20-GFP HA
Homo sapiens
source_name
JEKO-1-PI20-GFP HA
material type
Cell line
transgene
pINDUCER-20-GFP
treatment
Cells were transduced with lentiviral supernatants by spinfection for 90 minutes in the presence of polybrene. After 48 hour recovery, transduced cells were selected by G418 (10+ days). pINDUCER-20 transgene expression was induced with 100 ng/mL doxycycline 48 hours prior to cell harvest.
chip antibody
HA (Santa Cruz, catalog# sc-7392x, lot# E0913)