De-etiolating seedling from Gynandropsis gynandra
Gynandropsis gynandra
Gynandropsis gynandra seeds were sown directly from intact pods and germinated on moist filter papers in the dark at 32C for 24 hours. Germinated seeds were then transferred to half strength Murashige and Skoog (MS) medium with 0.8% (w/v) agar (pH 5.8) and grown for three days in a growth chamber at 26C. De-etiolation was induced by exposure to white light with a photon flux density (PFD) of 350 umol m-2 s -1 and photoperiod of 16 hours. Whole seedlings were harvested at 0.5, 2, 4 and 24 hours after illumination (starting at 8:00 with light cycle 6:00 to 22:00). Tissue was flash frozen in liquid nitrogen and stored at -80C prior to processing.
ecotype
Malaysia-1
age
3 days
dev_stage
De-etiolating seedling
geo_loc_name
Malaysia
tissue
Whole Seedling
BioSampleModel
Plant