TA076
Homo sapiens
Protocols: Ascites was collected from 30 patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg. Informed consent was obtained from all patients according to the protocols approved by the local ethical committe After Ficoll gradient centrifugation, tumor-associated macrophages (TAMs) were purified by CD14 MACS and lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit.
ENA-FIRST-PUBLIC
2015-06-01T16:20:34Z
ENA-LAST-UPDATE
2018-03-08T22:39:42Z
External Id
SAMEA3175749
INSDC center name
Institute for molecular and tumor biology Philipps University Marburg Germany
INSDC first public
2015-06-01T16:20:34Z
INSDC last update
2018-03-08T22:39:42Z
INSDC status
public
Submitter Id
E-MTAB-3167:TA076
broker name
ArrayExpress
cell type
tumor associated macrophages
clinical history
ascites ollected from 30 patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg
common name
human
individual
OC24TAM
sample name
E-MTAB-3167:TA076
scientific_name
Homo sapiens