72_278-ML-P-TF_XENO_ALDH-POS
Homo sapiens
Protocols: Colon cancer patient-derived xenograft (PDX) models models were processed to single cells and sorted by FACS (BD FACS Aria II) for ALDH activity (Aldefluor assay) and DAPI. ALDH Negative and ALDH Positive cells from each PDX model were collected. ALDH Negative and ALDH Positive cells from each PDX model were collected and lysed in RLT buffer and processed for RNA using the RNeasy Mini Plus RNA extraction kit (Qiagen). Samples were processed using Illumina's TrueSeq RNA protocol
ENA first public
2021-01-11
ENA last update
2020-12-11
External Id
SAMEA7705416
INSDC center alias
Charite - Universitatsmedizin Berlin
INSDC center name
Charite - Universitatsmedizin Berlin
INSDC first public
2021-01-11T04:04:54Z
INSDC last update
2020-12-11T13:59:59Z
INSDC status
public
Submitter Id
E-MTAB-8927:72_278-ML-P-TF_XENO_ALDH-POS
broker name
ArrayExpress
cell type
colorectal cancer cell
common name
human
disease
colon carcinoma
fraction
ALDH Positive
growth condition
Xenograft in female nude-/- mice
individual
Patient 278-ML-P-TF
sample name
E-MTAB-8927:72_278-ML-P-TF_XENO_ALDH-POS