5457sTS.Macaque.Cerebellum.9ypb.Male
Macaca mulatta
Protocols: The organs were dissected from 13 developmental stages (starting at e93 until 20-26 years of age). The prenatal brain was divided into two regions: forebrain together with the midbrain (referred to as the 'brain') and hindbrain (referred to as the 'cerebellum'). The postnatal 'brain' and 'cerebellum' samples comprise the dorsolateral prefrontal region of the cerebral cortex and lateral cerebellar cortex, respectively. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).
ENA-FIRST-PUBLIC
2018-12-30T17:01:52Z
ENA-LAST-UPDATE
2018-05-25T09:42:45Z
External Id
SAMEA4688412
INSDC center name
University of Heidelberg
INSDC first public
2018-12-30T17:01:52Z
INSDC last update
2018-05-25T09:42:45Z
INSDC status
public
Submitter Id
E-MTAB-6813:5457sTS.Macaque.Cerebellum.9ypb.Male
age
9
broker name
ArrayExpress
common name
Rhesus monkey
developmental stage
postnatal
genotype
wild type genotype
organism part
hindbrain
sample name
E-MTAB-6813:5457sTS.Macaque.Cerebellum.9ypb.Male
scientific_name
Macaca mulatta
sex
male