Cb-STs_AP
Homo sapiens
Protocols: Under signed informed consent, peripheral blood from healthy volunteers was obtained for the generation of antigen-specific T cells. Genomic DNA was isolated using a QIAamp DNA Mini Kit (Qiagen) and quantified with Qubit DNA Br kit according to manufactures' instructions. Genomic DNA was used for amplicon specific PCR using genome specific primers which flanked the expected on-target site for GR CRISPR/Cas9 target, and 7 (2 exonic and 5 intronic) in silico predicted potential offsite targets (named Off-site1-7). The 8 PCR products from each sample were pooled and 100ng of the final pool were used as input for the library construction with the NEBNext Ultra II Library Prep kit (New England Biolabs, #E7645). Library quantification was performed with the KAPA SYBR FAST Universal qPCR kit (KAPA Biosystems, #4824).
ENA first public
2021-01-14
ENA last update
2020-12-22
External Id
SAMEA7746877
INSDC center alias
Institute of Applied Biosciences (INAB), Centre for Research and Technology Hellas (CERTH), Thessaloniki, Greece
INSDC center name
Institute of Applied Biosciences (INAB), Centre for Research and Technology Hellas (CERTH), Thessaloniki, Greece
INSDC first public
2021-01-14T04:14:27Z
INSDC last update
2020-12-22T15:59:49Z
INSDC status
public
Submitter Id
E-MTAB-9968:Cb-STs_AP
broker name
ArrayExpress
cell type
pathogen-specific T-cells targeting CMV, EBV, BKV, adenovirus and Aspergillus fumigatus
common name
human
disease
normal
genetic modification
CRISPR/Cas9-mediated disruption of the glucocorticoid receptor
organism part
blood
sample name
E-MTAB-9968:Cb-STs_AP