5040sTS.Opossum.Liver.0dpb.Male
Monodelphis domestica
Protocols: The organs were dissected from 15 developmental stages (starting at e13.6 until P180). The dissected 'brain' samples correspond to the cerebral hemispheres (without the olfactory bulbs). Early 'cerebellum' samples correspond to the prepontine hindbrain-enriched brain region (until ~ P4) and from then onwards only to the cerebellum. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).
ENA-FIRST-PUBLIC
2018-12-31T17:02:27Z
ENA-LAST-UPDATE
2018-05-30T14:21:00Z
External Id
SAMEA4699365
INSDC center name
University of Heidelberg
INSDC first public
2018-12-31T17:02:27Z
INSDC last update
2018-05-30T14:21:00Z
INSDC status
public
Submitter Id
E-MTAB-6833:5040sTS.Opossum.Liver.0dpb.Male
age
0
broker name
ArrayExpress
common name
gray short-tailed opossum
developmental stage
postnatal
genotype
wild type genotype
organism part
liver
sample name
E-MTAB-6833:5040sTS.Opossum.Liver.0dpb.Male
scientific_name
Monodelphis domestica
sex
male