Nodsmall-N10_1
Medicago truncatula
Title
Nodsmall-N10
Genotype
Jemalong A17
Molecule
other
Extract_protocol
Plant roots and nodules were harvested and crushed with liquid nitrogen. small RNA were isolated from 100 mg of powder with the mirVana miRNA isolation kit (Ambion) according to the manufacturer s instructions. For the other libraries, total RNAs were extracted using 1 mL of Trizol per 50-100 mg of powder. Phenol-Chloroform separation, isopropanol precipitation, ethanol wash and RNA solubilization were performed according to the TRI REAGENT manufacturer s instructions. RNA integrity and quality have been checked thanks to the Agilent Bioanalyzer 2100
Growth_protocol
aeroponic growth (caisson medium)
Other_organism
Sinorhizobium meliloti 2011 taxid=1286640