MCP2_P1
Mus musculus
Protocols: Mice were sacrificed and spleens were surgically removed and were pressed through a 70 µm cell strainer (BD Falcon) for single cell suspension preparation. Blood was obtained with cardiac puncture or from submandibular vein. Peritoneal cells were obtained by washing peritoneal cavity with 3 - 4 ml of PBS twice. Red blood cells were lysed by ACK lysing buffer and cells were then washed with PBS with 2% of fetal bovine serum (FBS). All cells were resuspended in ice cold PBS with 2% FBS for FACs staining. TC-1, tumor cells derived from mouse lung epithelial cells and transformed by human papillomavirus were cultured in vitro. Cells were washed with PBS and 4 x 105 cells were inoculated subcutaneously in the right flank with 200 µl PBS. Twenty-six days later, tumors were surgically removed Splenocytes, peripheral blood cells, peritoneal cells and tumor cells were washed with PBS and were counted. Cells were centrifuged at 400 g for 5 min and supernatant was removed. Cells pellet (< 3 x 106 cells) was resuspend in 350 µl of RLT buffer (Qiagen). RNA was extracted with RNeasy Mini kit (Qiagen) according to manufacturer's protocol. mRNA library preparation was realized following manufacturer's recommendations (KAPA mRNA HyperPrep ROCHE). Library purity/integrity were assessed using an Agilent 2200 Tapestation (Agilent Technologies, Waldbrunn, Germany).
ENA-FIRST-PUBLIC
2020-07-09T04:03:54Z
ENA-LAST-UPDATE
2020-06-29T17:24:37Z
External Id
SAMEA7026349
INSDC center name
Ligue contre le Cancer
INSDC first public
2020-07-09T04:03:54Z
INSDC last update
2020-06-29T17:24:37Z
INSDC status
public
Submitter Id
E-MTAB-9271:MCP2_P1
age
8 to 10
broker name
ArrayExpress
clinical information
transfected with 4 x 105 cells TC-1 cells until tumor formation
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
2-1
organism part
peritoneum
sample name
E-MTAB-9271:MCP2_P1
scientific_name
Mus musculus
sex
female
strain
C57BL/6J