PR23.2d
Mus musculus
Protocols: Four to six week old mice were tail vein injected with AAV9 virus 1x10 11. At adulthood animals requiring 4-OHT ot tamoxifen were i.p. injected with 1 mg in 10 % ethanol and vegetable oil and collected 4 or 48 hours post injection. Mouse tissue was harvested from mice directly into liquid nitrogen. Total RNA was purified using PureLink RNA Mini Kit (Thermo Scientific) together with on column DNA digestion using PureLink DNase (Thermo Scientific). Then 1 to 5 µg of purified RNA was treated with Ribozero rRNA removal kit (Illumina) and ethanol precipitated. RNA quality and removal of rRNA were checked with the Agilent 2100 Bioanalyser (Agilent Technologies). Libraries for RNA-seq were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB) following manufacturers instructions.
ENA-FIRST-PUBLIC
2020-02-24T04:09:26Z
ENA-LAST-UPDATE
2019-10-24T10:57:36Z
External Id
SAMEA6116897
INSDC center name
UNIVERSITY OF CAMBRIDGE
INSDC first public
2020-02-24T04:09:26Z
INSDC last update
2019-10-24T10:57:36Z
INSDC status
public
Submitter Id
E-MTAB-8462:PR23.2d
age
8
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
AAV9-Ccnt1;Myh6-cre;R26CAG-c-MycERT2/+
individual
3
organism part
heart
sample name
E-MTAB-8462:PR23.2d
scientific_name
Mus musculus
sex
female
strain
mixed