m_d16
Homo sapiens
Protocols: Exosomes were isolated by serial ultra-centrifugation method. Briefly, 50 ml milk sample was diluted with an equal volume of 1X PBS (pH7.4) and centrifuged at 300 x g for 10 min at 4ºC to pellet the cells. Supernatant was further centrifuged at 3000 X g for 10 min at 4ºC followed by sequential filtrating of the supernatant through 1.2 µm, 0.8 µm, 0.45 µm and 0.2 µm filters (VWR®). Resultant filtrate was centrifuged at 16500 X g for 45 min at 4ºC and supernatant was again filtered using 0.2 µm filter followed by centrifugation at 12000 X g for 70 min at 4ºC to pellet exosomes. Exosome pellet was resuspended in 300 µl of 1X PBS. Total RNA was purified from the isolated exosomes using miRNeasy kit (Qiagen) according to the manufacturer's instructions. All libraries were prepared using the Illumina TruSeq Small RNA protocol following the manufacturer's instructions with 12 cycles of PCR amplification after ligation of the 3′ and 5′ adapters
ENA-FIRST-PUBLIC
2019-01-01T17:02:01Z
ENA-LAST-UPDATE
2018-10-25T10:37:30Z
External Id
SAMEA5044475
INSDC center name
Steno Diabetes Center Copenhagen
INSDC first public
2019-01-01T17:02:01Z
INSDC last update
2018-10-25T10:37:30Z
INSDC status
public
Submitter Id
E-MTAB-7336:m_d16
age
36
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
type I diabetes mellitus
individual
16
organism part
extracellular exosome
sample name
E-MTAB-7336:m_d16
sampling site
breast milk
scientific_name
Homo sapiens
sex
female