11_PLXR
Homo sapiens
Protocols: Melanoma specimens were obtained during surgical intervetions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research). Cell resistance was developed for 10uM vemurafenib or 50 nM trametinib. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol. DNA samples were quantified using Picogreen (Invitrogen Thermo Fisher Scientific, Carlsbad, CA, USA) and resolved by 1% agarose gel electrophoresis (30 min, 100 V) to confirm the presence of high molecular weight fragments. DNA samples were then prepared according to an Agilent SureSelect Human All Exome V6 kit (Agilent Technologies) which is a solution-based system utilizing ultra-long 120-mer biotinylated cRNA baits to capture regions of interest. Targeted regions were selected using magnetic streptavidin beads, amplified and loaded on the sequencer.
ENA-FIRST-PUBLIC
2019-06-15T04:02:31Z
ENA-LAST-UPDATE
2019-05-23T14:18:58Z
External Id
SAMEA5634669
INSDC center name
Department of Molecular Biology of Cancer, Medical University of Lodz, Poland
INSDC first public
2019-06-15T04:02:31Z
INSDC last update
2019-05-23T14:18:58Z
INSDC status
public
Submitter Id
E-MTAB-7991:11_PLXR
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
melanoma
genotype
wild type genotype
organism part
skin
sample name
E-MTAB-7991:11_PLXR
sampling site
neoplasm
scientific_name
Homo sapiens