Sample 7
Vibrio cholerae
Protocols: Bacterial strains were cultivated at 30C either in M9 Minimal medium or LB rich medium either to exponential phase (0.2 OD 650nm) or to stationnary phase (overnight culture). 1- FX85: EPV50 (WT) grown in M9 minimal medium supplemented with 0.4 % fructose to exponential phase (0.2 OD 650 nm). 2- FX86: EPV50 (WT) grown in M9 minimal medium supplemented with 0.4 % fructose to stationary phase (long overnight). 3- FX288: EGV140 (oriL3) grown in M9 minimal medium supplemented with 0.4 % fructose to exponential phase (0.2 OD 650 nm). 4- FX289:EGV140 (oriL3) grown in M9 minimal medium supplemented with 0.4 % fructose to stationary phase (long overnight). 5- FX290: EGV111 (oriR4) grown in M9 minimal medium supplemented with 0.4 % fructose to exponential phase (0.2 OD 650 nm). 6- FX291:EGV111 (oriR4) grown in M9 minimal medium supplemented with 0.4 % fructose to stationary phase (long overnight). 7- FX61:EMV01 (WT) grown in LB medium to exponential phase (0.2 OD 650nm) 8- FX62:EMV01 (WT) grown in LB medium to stationary phase (overnight) 9- FX286:EGV140 (oriL3) grown in LB medium to exponential phase (0.2 OD 650nm) 10- FX287:EGV140 (oriL3) grown in LB medium to stationary phase (overnight) 11- FX292:EGV111 (oriR4) grown in LB medium to exponential phase (0.2 OD 650nm) 12- FX49: MCH1 (WT monochromosome) grown in M9 minimal medium supplemented with 0.4 % fructose to exponential phase (0.2 OD 650 nm). 13- FX48: MCH1 (WT monochromosome) grown in M9 minimal medium supplemented with 0.4 % fructose to stationary phase (long overnight). 14- FX11: EGV369 (oriL3 monochromosome) grown in M9 minimal medium supplemented with 0.4 % fructose to exponential phase (0.2 OD 650 nm). 15- FX12: EGV366 (oriR4 monochromosome) grown in M9 minimal medium supplemented with 0.4 % fructose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. The libraries and the sequencing were performed by the High-throughput Sequencing facility of the I2BC (http://www.i2bc.paris-saclay.fr/spip.php?article399〈=en,CNRS, Gif-sur-Yvette, France). Genomic DNA libraries were made with the 'Nextera DNA library preparation kit' (Illumina) following the manufacturer's recommendations.
ENA-FIRST-PUBLIC
2018-12-31T17:02:29Z
ENA-LAST-UPDATE
2018-09-06T16:23:50Z
External Id
SAMEA4888508
INSDC center name
Evolution and maintenance of circular chromosomes,Genome biology department, Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universite-Paris-Sud, Universite Paris-Saclay, Gif-sur-Yvette,France,
INSDC first public
2018-12-31T17:02:29Z
INSDC last update
2018-09-06T16:23:50Z
INSDC status
public
Submitter Id
E-MTAB-7193:Sample 7
broker name
ArrayExpress
genotype
wild type genotype
growth condition
LB rich medium, exponential phase
sample name
E-MTAB-7193:Sample 7
scientific_name
Vibrio cholerae
strain
O1 biovar El Tor str. N16961