PGP1-Px458-CCR5
Homo sapiens
This sample is the experimental group. We selected protospacer sequences within CCR5 (C-C motif chemokine receptor 5 (gene/pseudogene)). Sequence of oligonucleotides used for targeting: sgRNA: ATGTCAGTCATAGTTAAGAC. Plasmid pSpCas9(BB)-2A-GFP (PX458) (Addgene Plasmid #48138) was used as an expression vector. Flow sorting of single cells after 2 days of transfection. Cells were cultured for a period of time to extract genome for high-throughput sequencing.
isolate
Caucasian
age
55
biomaterial_provider
Coriell Institute for Medical Research,Harvard Medical School,Harvard University
sex
male
tissue
Fibroblast
cell_line
PGP1
treatment
pX458 plasmid with sgRNA(ATGTCAGTCATAGTTAAGAC)
replication
11
BioSampleModel
Human