Day 159 Flowers Replicate 3
Salvia hispanica
Protocols: Tissues were collected from plant in greenhouse and immediately frozen in liquid nitrogen until nucleic acid extraction Total RNA was extracted from frozen plant tissue. Invitrogen Plant RNA Reagent, chloroform, isopropanol, and 8M Lithium Chloride, and DNAse  were used to obtain RNA. Qiagen RNA cleanup kit was used to remove any enzymes used in extraction.  Paired-end libraries were quantified by qPCR and prepared using an Illumina TruSeq Stranded RNA LT kit
isolate
not applicable
organism
Salvia hispanica
growth condition
green house study
developmental stage
day 159 post-germination
organism part
flower bud
ENA-FIRST-PUBLIC
2018-02-13
ENA-LAST-UPDATE
2017-02-22