Hues8-ABEmax-CFTR
Homo sapiens
This sample is the experimental group. We selected protospacer sequences within CFTR (CF transmembrane conductance regulator). Sequence of oligonucleotides used for targeting: sgRNA: TTGCAAGCTTCTTAAAGCAT. Plasmid pCMV_ABEmax_P2A_GFP (ABEmax) (Addgene Plasmid #112101) was used as an expression vector. Flow sorting of single cells after 2 days of transfection. Cells were cultured for a period of time to extract genome for high-throughput sequencing.
isolate
frozen cleavage- and blastocyst-stage human embryos produced by in vitro fertilization
age
not applicable
biomaterial_provider
Prof. Dr. Douglas A. Melton,Melton Lab,Harvard University
sex
male
tissue
embryonic
cell_line
HuES8
treatment
ABEmax plasmid with sgRNA(TTGCAAGCTTCTTAAAGCAT)
replication
2
BioSampleModel
Human