ROMEO2015.015.DNA_2015-05-27_StLaw_C_spring_river_water
freshwater metagenome
USGS 04264331 St. Lawrence River at Cornwall, Ontario, near Massena, NY
isolation_source
river water
collection_date
2015-05-27
geo_loc_name
USA: St. Lawrence River at Cornwall, Ontario, near Massena, NY
lat_lon
45.006336 N 74.795107 W
samp_collect_device
Collected into a 10L carboy filled with water passing through the Moses-Saunders Power Dam, consistent with water quality samples collected by the USGS on that day
samp_mat_process
Material pre-filtered with a 3.0 micron pore-size, 142mm diameter polycarbonate filter, and collected on a 0.22 micron pore-size Sterivex filter (Millipore, Burlington, MA). Genomic DNA from Thermus thermophilus HB8 was added (44 ng) prior extraction for quantification analyses, as per Satinsky et al. 2013 (https://doi.org/10.1016/B978-0-12-407863-5.00012-5). DNA was extracted using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI).
samp_size
4.7 L
isolate
StLaw_C_spring
replicate
replicate C
BioSampleModel
Metagenome or environmental