PGP1-ABEmax-CFTR
Homo sapiens
This sample is the experimental group. We selected protospacer sequences within CFTR (CF transmembrane conductance regulator). Sequence of oligonucleotides used for targeting: sgRNA: TTGCAAGCTTCTTAAAGCAT. Plasmid pCMV_ABEmax_P2A_GFP (ABEmax) (Addgene Plasmid #112101) was used as an expression vector. Flow sorting of single cells after 2 days of transfection. Cells were cultured for a period of time to extract genome for high-throughput sequencing.
isolate
Caucasian
age
55
biomaterial_provider
Coriell Institute for Medical Research,Harvard Medical School,Harvard University
sex
male
tissue
Fibroblast
cell_line
PGP1
treatment
ABEmax plasmid with sgRNA(TTGCAAGCTTCTTAAAGCAT)
replication
3
BioSampleModel
Human