A549 H3K4Me3 Input
Homo sapiens
Protocols: 90% RPMI1640 + 10% FBS For library, 40~50ng immunoprecipitated DNA were end-repaired and ligated adapters to the DNA fragments using NEBNext Ultra End-Repair/dA-Tailing Module and NEBNext Ultra Ligation Module (NEB, USA), respectively. DNA size selection was performed using 2% Agarose Gel, then 200~500bp DNA fragments were excised and purified using Qiagen Gel Extraction Kit (Qiagen, Germany). Each sample was amplified 14 cycles in a DNA thermal cycler using Q5 High-Fidelity DNA Polymerase (NEB, USA) and corresponding PCR Master Mix.
ENA-FIRST-PUBLIC
2016-06-01T17:01:04Z
ENA-LAST-UPDATE
2018-03-09T09:26:49Z
External Id
SAMEA3661575
INSDC center name
Shanghai Center for Systems Biomedicine, School of Biomedical Engineering and Bio-ID Center, Shanghai Jiao Tong University
INSDC first public
2016-06-01T17:01:04Z
INSDC last update
2018-03-09T09:26:49Z
INSDC status
public
Submitter Id
E-MTAB-3992:A549 H3K4Me3 Input
broker name
ArrayExpress
cell line
A549
cell type
lung cancer cell
common name
human
disease
non-small cell lung carcinoma
sample name
E-MTAB-3992:A549 H3K4Me3 Input
scientific_name
Homo sapiens