Ler_T2
Arabidopsis thaliana
Protocols: For RNA sequencing analysis, seedlings (12 DAS for Col-0 and Ey15-2 and 13 DAS for WalhaesB4, ICE97, ICE138, ICE75, Ler-0, Yeg-1, Sha, ICE153) were harvested in RNA ice-later solution (AM7030; Ambion) and incubated at -20°C for at least a week. Leaf 6 was micro-dissected on a cold plate with dry ice under a stereomicroscope, and frozen in liquid nitrogen. RNA was extracted with Trizol (Invitrogen) according to the manufacturer's instructions and the RNeasy kit (Qiagen) with on-column DNase (Qiagen) digestion. RNA was quantified and the quality was checked with a 2100 Bioanalyzer (Agilent). Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, polyA containing mRNA molecules are reverse transcribed, double-stranded cDNA is generated and adapters are ligated.
ENA first public
2017-12-14
ENA last update
2016-02-29
External Id
SAMEA3882061
INSDC center alias
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
INSDC center name
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
INSDC first public
2017-12-14T17:02:56Z
INSDC last update
2016-02-29T14:27:21Z
INSDC status
public
Submitter Id
E-MTAB-4508:Ler_T2
broker name
ArrayExpress
common name
thale cress
ecotype
Landsberg erecta
genetic modification
GA20ox1 OE
organism part
Leaf 6
sample name
E-MTAB-4508:Ler_T2