HR05_IB-1
Oryza sativa
In this test, 9 rectangular plastic basins (length * width * height: 40 cm * 20 cm * 10 cm) with soggy soil were set. In each basin, 10 seeds of GH308, HR05 and TN1 (30 seeds in total) were cultivated in the soil and arranged in three lines as Fig 1 displayed. To ensure the 100% of emergence rate, all tested seeds were healthy and soaked in water for 24 hours before sowing.
ENA-FIRST-PUBLIC
2020-07-09T08:05:09Z
ENA-LAST-UPDATE
2020-07-01T07:30:31Z
External Id
SAMEA7030888
INSDC center name
Fujian Agriculture and Forestry University
INSDC first public
2020-07-09T08:05:09Z
INSDC last update
2020-07-01T07:30:31Z
INSDC status
public
Submitter Id
s7-1
collection date
2017-09
common name
rice
estimated size
400M
geographic location (country and/or sea)
China
geographic location (latitude)
26.00697703
geographic location (longitude)
119.3604179
isolation and growth condition
To ensure the 100% of emergence rate, all tested seeds were healthy and soaked in water for 24 hours before sowing. The 9 basins of seeds would kept grow in greenhouse for 10 days, with conditions of 14:10 photoperiod and temperature of 28��1��. Then the 9 basins of seedlings were divided into 3 groups, 2 treatments and 1 control would be set as follow: Group 1, for each basin, 300 individuals of 3-4 instars of planthopper nymphs were released and they could freely move and feed on the 30 seedlings for next 24 hours. Then 5 stems of each rice cultivar were cut, put into three cryo tubes and store in a -80 �� refrigerator rapidly. The tubes were labeled as GH308-IB, HR05-IB and TN1-IB. The tested brown planthoppers were reared indoor for at least 10 generations, feed with seedlings of TN1during their whole life; Group 2, the stems of each seedling would be prick 10 times randomly every hour in next 24 hours, as simulation of insect piercing, then 5 stems of each rice cultivar were cut, put into three cryo tubes and store in a -80 �� refrigerator rapidly (labeled as GH308-MIB, HR05-MIB and TN1-MIB); Group 3, the last three basins was set as control and treated with nothing. After 24 hours, the stems were collected as above did (labeled as GH308-CK, HR05-CK and TN1-CK)
number of replicons
1
plant developmental stage
matrue
plant growth medium
soil
plant structure
leaf
sample name
s7-1
scientific_name
Oryza sativa