gln3(i-BuOH)_rep1
Saccharomyces cerevisiae
Protocols: Cells were harvested at 3,000 × g for 5 min at room temperature. Wild-type BY4741 and BY4741 with gln3 deletion strains were pre-cultivated in liquid SC medium at 30°C for 24 h. Each pre-culture was inoculated into fresh liquid SC medium made inhouse with or without 1.3% (v/v) isobutanol to obtain a starting OD600 of 0.1. Cultivation was performed in test tubes with screw caps at 30°C for 12 h with 240 rpm shaking. Total RNA was extracted from each culture with Isogen-LS (Nippon Gene, Tokyo, Japan). RNA integrity was evaluated for quality control with an Agilent Bioanalyzer 2,100 using Agilent RNA 6,000 Nano Kit (Agilent technologies, Santa Clara, CA). cDNA library preparation was performed using KAPA RNA HyperPrep Kit Illumina Platforms (Kapa Biosystems, Wilmington, MA). Prepared cDNA libraries were validated with an Agilent Bioanalyzer 2,100 using an Agilent High Sensitivity DNA Kit (Agilent Technologies).
ENA-FIRST-PUBLIC
2019-11-14T04:04:33Z
ENA-LAST-UPDATE
2019-07-23T15:39:45Z
External Id
SAMEA5806238
INSDC center name
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
INSDC first public
2019-11-14T04:04:33Z
INSDC last update
2019-07-23T15:39:45Z
INSDC status
public
Submitter Id
E-MTAB-8175:gln3(i-BuOH)_rep1
age
12
broker name
ArrayExpress
cell line
BY4741
common name
baker's yeast
genotype
gln3 deletion
growth condition
SC medium with 1.3% (v/v) isobutanol
organism part
whole organism
sample name
E-MTAB-8175:gln3(i-BuOH)_rep1
scientific_name
Saccharomyces cerevisiae