Sample 291
Homo sapiens
Protocols: Tonsils were obtained from pediatric tonsillectomies. Tissues were disrupted by mechanical means and pressed through a stainless steel mesh to obtain a single-cell suspension. Mononuclear cells were isolated from a Ficoll-Hypaque density gradient. Tonsil mononuclear cells were depleted of T and B cells by FITC-conjugated anti-CD3, anti-CD19 and anti-FITC micro-beads and passed over an LD magnetic column. Cells were subsequently sorted to purity using a FACSAria and antibodies against lineage (CD3, CD19, CD20, CD94, CD34, CD16,CD14, TCRαβ, TCRγδ), HLA-DR, CD11c, CCR7, CD123 and BDCA2. eTACs were sorted as Lin-CD123-BDCA2-HLADR+CCR7+, cDCs as Lin-CD123-BDCA2-HLADR+CD11c+ and pDCs as Lin-HLADR+CD123+BDCA2+. Cells were incubated with an FcR block prior to antibody staining. Consent was not given to record age and gender information of donors. Single cell cDNA was generated using the SMART-seq2 protocol. Single cell cDNA was generated using the SMART-seq2 protocol.
ENA-FIRST-PUBLIC
2019-01-14T17:03:31Z
ENA-LAST-UPDATE
2018-10-25T16:32:13Z
External Id
SAMEA5046918
INSDC center name
Wellcome Sanger Institute
INSDC first public
2019-01-14T17:03:31Z
INSDC last update
2018-10-25T16:32:13Z
INSDC status
public
Submitter Id
E-MTAB-7381:Sample 291
broker name
ArrayExpress
cell type
extra thymic aire-expressing cells
common name
human
developmental stage
juvenile stage
disease
tonsilitis
individual
Donor B
inferred cell type
extra thymic aire-expressing cells
organism part
tonsil
phenotype
CD45+HLADR+CD127+CD40+EpCAM+
post analysis well quality
pass
sample name
E-MTAB-7381:Sample 291
scientific_name
Homo sapiens
single cell quality
OK