Hues8-Px458-CCR5
Homo sapiens
This sample is the experimental group. We selected protospacer sequences within CCR5 (C-C motif chemokine receptor 5 (gene/pseudogene)). Sequence of oligonucleotides used for targeting: sgRNA: ATGTCAGTCATAGTTAAGAC. Plasmid pSpCas9(BB)-2A-GFP (PX458) (Addgene Plasmid #48138) was used as an expression vector. Flow sorting of single cells after 2 days of transfection. Cells were cultured for a period of time to extract genome for high-throughput sequencing.
isolate
frozen cleavage- and blastocyst-stage human embryos produced by in vitro fertilization
age
not applicable
biomaterial_provider
Prof. Dr. Douglas A. Melton,Melton Lab,Harvard University
sex
male
tissue
embryonic
cell_line
HuES8
treatment
pX458 plasmid with sgRNA(ATGTCAGTCATAGTTAAGAC)
replication
12
BioSampleModel
Human