Generic sample from Pinus taeda
Pinus taeda
STRAIN
half sibling family from 7-56
BODYSITE
stem
library type
standard
library description
Seedlings were grown in a growth chamber until 7 mo. of age. Ten seedlings per treatment were used. For treatments 1-7, stems were cut at base above ground. In treatments 1-6, stems were placed in beakers containing 500 mL of the chemicals listed. Treatments 8-10 were carried out on seedlings still in potting cones. The pooled RNA sample contains equivalent amounts of total RNA isolated from each of the nine treatments. 1. 0.5mg/mL Clycoheximide 2. 100uM Gibberellic Acid 3. 1% H2O2 4. 100uM Indole-3-acetic acid 5. 100uM Methyl jasmonate 6. 100uM Kinetin 7. Dessication (cut stems placed on bench) 8. Cold Shock -20C 9. Heat Shock 45C 10. Wounding (stem crushed with pliers)