Snyder_DAF12_GFP_L3_rep2 extraction2_seq1 aliquote 1
Caenorhabditis elegans
source_name
Snyder_DAF12_GFP_L3_rep2
strain
OP167(official name : OP167 genotype : unc119(ed3); wgIs167(daf-12::TY1 EGFP FLAG; unc119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The DAF-12::EGFP fusion protein is expressed in the correct daf-12 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the DAF-12 transcription factor. made_by : S Kim )
developmental stage
L3
genotype
unc119(ed3);wgIs167(daf-12::TY1 EGFP FLAG;unc119)
Sex
hermaphrodite