Generic sample from Pinus taeda
Pinus taeda
BODYSITE
callus derived from needle tissue
library type
standard
library description
Callus cells were grown in liquid medium (100 mL cultures) to a point where maximum cell density was obtained- 14 days. Elicitors were dissolved in culture medium and added to each culture to give the final concentrations listed below. Cultures were then incubated for another 4 hr. Following cell harvest and RNA isolation, equivalent amounts of total RNA were pooled and the pooled sample was treated with Ambion RNase free DNAse. 1. Methyl Jasmonate (100uM) 2. Gibbberellic Acid (100uM) 3. Kinetin (100uM) 4. Cycloheximide (0.5mg/mL) 5. Indole-3-acetic Acid (100uM) 6. Nitrophenol (100uM) 7. Sodium Azide (100uM) 8. Colchicine (100uM) 9. Sodium (meta) vanadate (100uM) 10. Hydrogen Peroxide (0.01%) 11. Chitin (1mg/mL) 12. Chitosan (100ug/mL) 13. Untreated Sirex noctilio venom glands (125mg/mL) 14. Boiled Sirex noctilio venom glands (125mg/mL)