Day2_Luciferase control
Mus musculus
strain
129_TNG MKOS
dev_stage
Embryonic
sex
not applicable
tissue
Embryonic Fibroblasts
cell_type
Fibroblast: reprogramming
growth_protocol
3 to 15% of TNG_MKOS MEFs together with 97 to 85% WT MEFs were transduced with retroviral particles expressing transgenes for 4 hrs and grown in GMEM+1000 units of LIF, 1ug/ml Doxycycline, 10 ug/ml Vitamin C, 10% FBS, 1X non-essential medium, 1X pen/strep, 0.1 mM 2mercaptoethanol, 2mM L- glutamine. mOrange positive cells for the first five days and Nanog-GFP positive for day 15 were collected by FACS for sample preparation.
sample_type
SRA
treatment
Around 350,000 mOrange positive cells were sorted by flow cytometry for preparing mRNA-seq samples as mentioned in Mittal et al., Nat Commun 2017; 8: 457.
BioSampleModel
Model organism or animal