Plant sample from Tomato shoot-tip endophytic bacterial microbiome
endophyte metagenome
The study was undertaken to get an estimate of the gross endophytic bacterial diversity in the shoot tip tissues four representative plants, namely, tomato, watermelon, periwinkle and maize adopting cultivation versus 16S rRNA gene taxonomic profiling. Cultivation based approach showed very low or no cfu. On the other hand, 16S rRNA gene V3-V4 profiling revealed a high diversity of bacteria which indicated them prevailing in an uncultivable form. Thus, this study brings out the prevalence of a high diversity of cultivation recalcitrant endophytic bacteria (CREB) in support of the observations on intracellular colonization of shoot-tip tissues by abundant and diverse endophytic bacteria.