Paramecium tetraurelia PacBio RSII sequencing T50 stage (Library 1)
Paramecium tetraurelia
Total genomic DNA was extracted from wild type Paramecium tetraurelia cells (strain 51 mating type 7) at a late developmental stage (T50) of the self-fertilization process of autogamy. 2 SMRTbell libraries were prepared (size selection step using a 15kb (Library 1) or 13kb (Library 2) cutoff) then were sequenced on 16 SMRTcells on the RSII instrument.