K23_CD62L_1
Mus musculus
Protocols: CD8+CD44lowCD62L+ cells were isolated from Egr2/3 DKO or Egr2 Kin mice by FACS RNA was isolated and purified using TRIzol reagent (Life Technologies) according to the manufacturer's instructions RNA from T cells was processed for directional mRNA-Seq library construction using the NEBNext mRNA isolation kit and NEBNext Ultra II Directional RNA Library prep kit (New England Biolabs) according to the manufacturer's protocols