Paramecium tetraurelia PacBio RSII sequencing T50 stage (Library 1+2, ipdSummary analysis)
Paramecium tetraurelia
Total genomic DNA was extracted from wild type Paramecium tetraurelia cells (strain 51 mating type 7) at a late developmental stage (T50) of the self-fertilization process of autogamy. 2 SMRTbell libraries were prepared (size selection step using a 15kb (Library 1) or 13kb (Library 2) cutoff) then were sequenced on 16 SMRTcells on the RSII instrument. After alignment, sequencing data from all SMRTcells was merged. Then, 6mA were detected using the ipdSummary.py script.