Sample 2
Mus musculus
Protocols: Lymph node samples were homogenized in 600ul RLT lysis buffer (Qiagen) using a Precellys Homogenizer Precellys. RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA integrity was confirmed using Agilent's 2100 Bioanalyser. Libraries were prepared using the KAPA mRNA Hyper Prep Kit (Roche), following manufacturer's instructions.