Mouse gut metagenome of 16S rRNA genes from feces of 100_g/kg linaclotide administered adenine-induced renal failure mouse(biological replicate2)
mouse gut metagenome
Male C57BL/6 mice were fed with a normal diet (type CE-2; Clea Japan). At age 8 weeks, mice were divided randomly into the control and adenine groups. For the control group, the normal diet was continued. For the adenine group, a CE-2 diet containing 0.2% adenine (Wako Laboratory chemicals, Japan) was given for 6 weeks. After 6-week adenine feeding, the food was switched to the normal diet and then linaclotide (10 or 100 ug/kg, Toronto Research Chemicals) at a volume of 100 uL saline was administered to the mice by daily gavage at night for 14 days. Then, the mice were sacrificed and blood and tissues were obtained. One day prior to sacrifice, urine and feces were collected.