Parus major transcriptome sequencing
Parus major
Ten unrelated great tit fledglings were sampled from across the Wytham Woods, UK, population and brain, heart, kidney, liver, muscle, pancreas, skin and testes/ovaries dissected from each individual at between three and 23 minutes after death. Each chick was sexed at sampling and sex was later confirmed molecularly with the Z-002A marker. Tissues were stored in RNALater (Ambion) to prevent RNA degradation. RNA was extracted using the TRIzol* (Invitrogen) method. RNA quantity and degradation was tested using a Nanodrop (Agilent) with each tissue yielding a mean (SE) of 44.4 (7.5) ug of RNA with mean (SE) RNA integrity number (RIN) of 8.37 (0.21). For each tissue 1ug of RNA from each bird was pooled, i.e. to make 8 pools, each containing 10ug of RNA.