Metagenome of the 0.22 micron fraction from Freshwater Reservoir Amadorio
freshwater metagenome
The study aims to describe the microbiota of a freshwater reservoir using high throughput sequencing. The reservoir is called Amadorio and is located in the region of Alicante, Spain. 50L of sample was passed through a series of 50,5 and 0.22 micron filters. DNA was extracted from the 0.22 micron filter. A fosmid library was constructed with this DNA (60 96-well plates), ca. 5760 fosmids. Fosmids were pooled in batches of four plates (384 fosmids each) and a total of 15 samples were sent for sequencing by Illumina HiSeq 2000.