Brachypodium distachyon (Bd21-3) and rvr1 transcriptome
Brachypodium distachyon
This study was done in order to explore the specificity of a rapid flowering mutant in Brachypodium distachyon named REPRESSOR OF VERNALIZATION1 (RVR1) which involved in establishing a vernalization requirement. We were interested to compare the non-vernalized rvr1 transcriptome with that of vernalized wild type plants. In order to obtain vernalized and non-vernalized plants that were developmentally matched, the vernalized sampled were vernalized as imbibed seed in soil in 8hour short days. At the end of the cold treatment, non-vernalized samples were planted and all samples were grown to the third leaf stage in 8 hour short days and the fully expaned third leaf was harvested in the middle of the photoperiod.