Female-E2
Mus musculus
Protocols: Previously generated Sox9f/f; Sox8-/- mice were bred to Wt1-CreERT2 mice and the resulting double heterozygous offspring harboring the Cre allele was backcrossed to Sox9f/f; Sox8-/- mice to obtain heterozygous and homozygous compound Sox9; Sox8 conditional mutants. The same mating scheme was followed with the Sox9-CreERT2 mouse line. To report CRE activity, an R26R-EYFP reporter allele was crossed into Wt1-CreERT2; Sox9f/f; Sox8-/- and Sox9-CreERT2; Sox9f/f; Sox8-/- mice. Both testes were extracted from six 5 months old, TX-treated mutant males (three Wt1- CreERT2; Sox9f/f; Sox8-/- and three Sox9-CreERT2; Sox9f/f; Sox8-/-). As controls, both gonads were also extracted from four 5 months old control male mice (two TX-treated and two untreated) as well as from two 4-5 months old normal females. All TX-treated mice were euthanized three months after the initiation of diet TX-treatment for one month. The two gonads of each individual were pooled, homogenized in 1ml of RNAzol (Molecular Research Center, Inc.) per 100mg of tissue and the total RNAs were then individually purified from the twelve samples following the RNAzol manufacturer's instructions. After successfully passing Macrogen Inc. quality check, the twelve RNAs were paired-end sequenced