wt ESC1
Mus musculus
Protocols: E14Tg2a wt and Obs3 mutant ESCs were routinely grown in GMEM medium plus 12% FBS (Hyclone) and LIF (Millipore). RNA was extracted with Trizol (Invitrogen) Indexed sequencing libraries were generated from 1ug RNA, using the TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero ribosomal RNA reduction chemistry (Illumina, San Diego, CA), according to the manufacturer's instructions.