Ostrinia scapulalis reference transcriptome
Ostrinia scapulalis
This BioSample refers to the read libraries and the de novo reference transcriptome of the Adzuki bean borer, Ostrinia scapulalis. Collected diapausing larvae were reared in the laboratory to obtain fresh tissues from various developmental stages: eggs, larval whole body and hemolymph from the fifth instar and heads/thorax from male and female adults. In total, 7 RNA extracts were prepared. Indexed cDNA libraries (insert sizes 150-200 bp) were constructed for each developmental stage and tissue. The libraries were sequenced with an Illumina HiSeq 2000 sequencer using the paired-end protocol. After read cleaning and normalization steps a reference transcriptome was generated, consisting of 44,564 transcripts.