Betaarterivirus suid 1 DV strain, 4 different forms
Betaarterivirus suid 1
In this biosample, corresponding to the sequencing of the Porcilis PRRS vaccine batch A142DB01, buy in 2012 and then amplified in MARC-145 cells for 2 passages, 4 different forms of the DV vaccine strain were present: FULL-LENGTH form (Biosample ID SAMN18063686 - Accession No MW674755 - no deletion - minority representation), LONG-DEL form (Biosample ID SAMN18065256 - Accession No MW674756 - deletion of 222 nt from 2216 to 2437 compared to the FULL-LENGTH form positions - majority representation), SHORT-DEL form (Biosample ID SAMN18065501 - Accession No MW674757 - deletion of 135 nt from 2231 to 2365 compared to the FULL-LENGTH form positions - intermediate representation) and SHIFT-DEL form (Biosample SAMN18065738 - No accession MW674758 - deletion of 92 nt from 2344 to 2435, then of 61 nt from 2446 to 2506 compared to the FULL-LENGTH form positions - minority representation). Similar results were obtained sequencing the Porcilis PRRS vaccine batch A207CB01, buy in 2016, without cell culture amplification.