ES_mock_antiMGA
Mus musculus
Protocols: Mouse J1 ES Cells were cultivated feeder-cell free on gelatin-coated plates in DMEM + GlutaMax (Gibco), supplemented with 15% fetal bovine serum (Biochrom), 1% non-essential amino acids (Gibco), 1% Penicillin/Streptomycin (Sigma), 50mM beta-Mecraptoethanol and 1000U/ml ESGRO leukmia inhibitory factor (Merck Millipore). ChIP was performed using the One Day ChIP Kit (Diagenode) according to manufacturer's instructions. Three to four individual ChIPs were pooled and purifed on QIAquick columns (Qiagen). Five nanograms of precipitated DNA were used for indexd deep sequency library preperation using the Microplex library preparation kit v2 (Diagenode). Libraries were purified on AMPure magnetic beads (Beckman Coulter).]
ENA first public
2018-01-01
ENA last update
2017-08-24
External Id
SAMEA104225673
INSDC center alias
Institute of Molecular Biology and Tumor Resarch (IMT), Philipps-University of Marburg, Marburg, Germany
INSDC center name
Institute of Molecular Biology and Tumor Resarch (IMT), Philipps-University of Marburg, Marburg, Germany
INSDC first public
2018-01-01T17:01:42Z
INSDC last update
2017-08-24T17:01:08Z
INSDC status
public
Submitter Id
E-MTAB-6007:ES_mock_antiMGA
broker name
ArrayExpress
cell line
J1
cell type
embryonic stem cell
common name
house mouse
genotype
wild type genotype
sample name
E-MTAB-6007:ES_mock_antiMGA