151013_FR07961465
Homo sapiens
Human term placental tissues were cut and single cell suspensions prepared. Hematopoietic populations were depleted by using Dynabeads® CD45. To enrich endothelial cells CD45- cells were subjected to CD34 MicroBeads separation. CD45-CD34+ cells were sorted using the FACS machine. Upon analysis using a pulse geometry gate cell doublets were eliminated and the CD34+Hi CD45- population was gated and sorted into 4 populations according to the level of CD31 expression (CD31- CD31Low CD31Int and CD31Hi). Populations of interest were sorted directly into 100% FBS and then cultured individually in collagen -coated 48-well plates with EGM-2. After 2 weeks culture RNA-sequencing was performed on the three placental populations with high colony forming potential (CD45-CD34+CD31- CD45-CD34+CD31Low and CD45-CD34+CD31Int).
isolate
Total RNA
age
Term placenta
biomaterial_provider
Abbas Shafiee PhD; Postdoctoral Research Fellow| Institute of Health and Biomedical Innovation | Queensland University of Technology | 60 Musk Ave Kelvin Grove QLD 4059 | T:+61 4 0684 1866 | E:a.shafiee@qut.edu.au
sex
Missing
tissue
Placenta
sample_type
64Hi
BioSampleModel
Human