AO.IP
Homo sapiens
Protocols: PDAC tissue from surgical samples was fragmented, mixed with 100 μL of Matrigel and implanted with a trocar (10 gauge; Innovative Research of America, Sarasota, FL) in the subcutaneous right upper flank of an anesthetized and disinfected male NMRI-nude mouse. Samples obtained from EUS-FNA were mixed with 100 μL of Matrigel (BD Biosciences, Franklin Lakes, NJ) and injected in the upper right flank of a male nude mice (Swiss Nude Mouse Crl: NU(lco)-Foxn1nu; Charles River Laboratories, Wilmington, MA) for the first implantation. When xenografts reached 1 cm3, these were removed and passed to NMRI-nude mice in the same manner as the surgical samples. All animal experiments were conducted in accordance with institutional guidelines and were approved by the “Plateforme de Stabulation et d'Expérimentation Animale” (PSEA, Scientific Park of Luminy, Marseille). RNA was extracted using RNeasy mini kit with optional on column DNA digestion (Qiagen). RNA libraries were prepared (Illumina TruSeq mRNA v2) and run on the Illumina High Seq-2000 for 101 bp paired end reads
ENA first public
2017-12-30
ENA last update
2017-06-28
External Id
SAMEA104143853
INSDC center alias
Mayo Clinic
INSDC center name
Mayo Clinic
INSDC first public
2017-12-30T17:01:52Z
INSDC last update
2017-06-28T10:14:18Z
INSDC status
public
Submitter Id
E-MTAB-5639:AO.IP
broker name
ArrayExpress
common name
human
disease
pancreatic adenocarcinoma
growth condition
xenograft in nude mouse
individual
AO.IP
organism part
neoplasm
phenotype
classical
sample name
E-MTAB-5639:AO.IP