Fig3CS3A_DDAAA_Smc3HA_IP
Saccharomyces cerevisiae
source_name
Saccharomyces cerevisiae cells
sac_cer strain
K26611
can_gla strain
K23308
antibody
anti-HA atibody (3F10, Roche)
genotype
Strain K26611 (containing 3XminiAID tagged-smc3 and smc1DD in their respective endogenous locus, and smc3AAA mutant expressed from an ectopic locus)
growth
arrested in G1, synthetic auxin (indole-3-acetic acid) was added to 1 mM 30 min before release. The culture was released into YPD containing 1mM auxin and nocodazole to arrest the cultures in G2