16S rRNA analysis of DNA extracted from autoclaved, deionized water which was used as negative control
synthetic metagenome
Bacterial 16S rRNA analysis was done with DNA extracted from autoclaved, deionized water. The aim was to identify microbial contaminations that are introduced during DNA extraction and/or amplicon preparation. DNA extraction was performed in parallel to the extraction of DNA from coal tar polluted groundwater and BTEX-degrading enrichment cultures. Microbial communities were analyzed by 16S rRNA amplicon sequencing of the V3/V4 region using a Illumina MiSeq 2x300 platform.
ENA-FIRST-PUBLIC
2018-01-02T17:02:34Z
ENA-LAST-UPDATE
2017-03-13T11:22:08Z
External Id
SAMEA103910182
INSDC center name
FRIEDRICH SCHILLER UNIVERSITY JENA
INSDC first public
2018-01-02T17:02:34Z
INSDC last update
2017-03-13T11:22:08Z
INSDC status
public
Submitter Id
No-cells control
collection date
2015-05
environment (biome)
freshwater biome
environment (feature)
research facility
environment (material)
sterile water
geographic location (country and/or sea)
Germany
geographic location (latitude)
50°55'50.9N
geographic location (longitude)
11°35'01.1E
investigation type
metagenome
miscellaneous environmental package
miscellaneous natural or artificial environment
project name
Microbial communities of coal tar polluted groundwater
sample name
No-cells control
scientific_name
synthetic metagenome
sequencing method
Illumina MiSeq 2x300 (Reagent Kit v3)