Sample 40
Hordeum vulgare subsp. vulgare
Protocols: Main shoot apex enriched tissues were harvested and pooled for three distinct developmental stages, the vegetative stage (W1.0), the spikelet initiation stage (W2.0) and the anther primordium stage (W3.5) (Waddington et al., 1983). The samples were harvested 2 hours before dark under both long day and short day conditions. To enrich shoot apex specific mRNA, leaves surrounding the main shoot apex were removed manually using a microsurgical stab knife (5-mm blade at 15° [SSC#72-1551]). The enriched main shoot apex tissue was cut from the base of the main shoot apex and still included leaf primordia. At least 10 main apex tissues were pooled for each of the three biological replicates per time point. All samples harvested for RNA extraction were frozen immediately in liquid nitrogen and stored at -80°C before RNA extraction. Wild type Bonus, two allelic praematurum-c/mat-c mutants including mat-c.907 (NGB 116862) and mat-c.943 (NGB 117474) were grown under long (16h/8h, light/dark) and short day (8h/16h, light/dark) conditions in a controlled plant chamber (22°C day/18°C night) and in in 96-well trays using “Mini Tray” (Einheitserde®) soil. To synchronize germination, the trays were stratified in dark at 4°C for three days. Total mRNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and further purified using an RNA easy Micro Kit (Qiagen). The residual DNA was removed using a DNA-free kit (Ambion) and the quality of the RNA was assessed using a bioanalyzer (Agilent 2100 Bioanalyzer). The Illumina cDNA libraries were prepared according to the TruSeq RNA sample preparation (version 2; Illumina).
ENA-FIRST-PUBLIC
2019-04-23T17:03:42Z
ENA-LAST-UPDATE
2019-03-22T11:00:29Z
External Id
SAMEA5528401
INSDC center name
Institute for Plant Genetics, Heinrich Heine University Duesseldorf; Max Planck Institute for Plant Breeding Research
INSDC first public
2019-04-23T17:03:42Z
INSDC last update
2019-03-22T11:00:29Z
INSDC status
public
Submitter Id
E-MTAB-7807:Sample 40
broker name
ArrayExpress
common name
domesticated barley
cultivar
Bonus
developmental stage
Waddington Stage 2
genotype
mat.c.943
growth condition
Long-day photoperiod
organism part
shoot apex
replicate
2
sample name
E-MTAB-7807:Sample 40
scientific_name
Hordeum vulgare subsp. vulgare