Fig3CS3A_WT_Smc3HA_WCE
Saccharomyces cerevisiae
source_name
Saccharomyces cerevisiae cells
sac_cer strain
K26797
can_gla strain
K23308
antibody
none (input)
genotype
Strain K26797 (containing 3XminiAID-tagged smc3 in the endogenous locus and Smc3 wild type expressed from an ectopic locus)
growth
arrested in G1, synthetic auxin (indole-3-acetic acid) was added to 1 mM 30 min before release. The culture was released into YPD containing 1mM auxin and nocodazole to arrest the cultures in G2