Homo sapiens
Each BAM formatted entry describes a non-mitochondrial, deduplicated ATAC-seq fragment, including the start and end genomic coordinates as well as an RG tag that indicates the single cell of origin and nano-well chip ID (RG = <chip ID>_<cell ID>). The RG tag directly corresponds to the name of the associated raw sequence read files (fastq format).
isolate
unknown
age
25-35
biomaterial_provider
AllCells; 5858 Horton Street, Suite 360, Emeryville, CA 94608, United States
sex
male
tissue
Peripheral blood CD4+ T cells
Sample isolation
Monocytes, T cells, CD4 T cell, CD8 T cells and B cells were isolated from whole blood (AllCells, CA, USA) using EasySep Direct Human cell isolation kits (STEMCELL Technologies, MA, USA) according to the manufacturers protocol. Isolated PBMCs (AllCells, CA, USA) were thawed in RPMI and washed once in media before staining the cells as described below. All human cells were obtained from AllCells with explicit consent to publish data for broad genomic release.
BioSampleModel
Human