Max_MycOE_ctr_1
Drosophila melanogaster
Protocols: 48 hours after the heat-shock, wing imaginal discs were dissected into Qiazol & stored at -80C Drosophila melanogaster were grown on standard food at 25C larvae were subjected to a 2-hour 37C heat-shock (48 hours before collection) total RNA was extracted from wing imaginal discs using the Qiazol method cDNAs were prepared with NEBNext Poly(A) mRNA Magnetic Isolation Module (Illumina). Sequencing libraries were prepared with the NEBnext mRNA Library Prep Master Mix set for Illumina.
ENA first public
2017-10-17
ENA last update
2017-02-07
External Id
SAMEA80338918
INSDC center alias
Department of Biochemistry and Molecular Biology, Biocenter, University of Wurzburg
INSDC center name
Department of Biochemistry and Molecular Biology, Biocenter, University of Wurzburg
INSDC first public
2017-10-17T17:02:31Z
INSDC last update
2017-02-07T13:34:09Z
INSDC status
public
Submitter Id
E-MTAB-5470:Max_MycOE_ctr_1
broker name
ArrayExpress
common name
fruit fly
developmental stage
L3 larva
genotype
hs-FLP / actin5C-FRT-stop-FRT-GAL4 / Max-/- / UAS-Myc
organism part
wing disc
phenotype
Max knockout; Myc overepxression
sample name
E-MTAB-5470:Max_MycOE_ctr_1