Sample 9 MSA_SN
Mus musculus
Protocols: C57BL/6 wild type and multiple system atrophy transgenic mice (Tg(Plp1-SNCA)1Haa strain backcrossed in C57BL/6 in over 20 generations) at an age of 2-3 months were used in this study. The transgenic mice have a transgenic insertion of the human wild-type alpha synuclein driven by the proteolipid protein promoter. Transgenic protein is detected in oligodendrocytes but in no other brain cell type. The mice were sacrificed by cervical dislocation, brains were quickly extracted and the substantia nigra and striatum were dissected on ice. Three pools of substantia nigra and striatum, respectively, from 5 mice each were prepared and frozen in liquid nitrogen. Total RNA was isolated using Tri-Reagent (Sigma-Aldrich, St. Louis, Missouri, USA) according to the manufacturer's instructions and dissolved in DEPC-water. RNA was then submitted to polyA-enrichment (Dynabeads mRNA Purification Kit, Life technologies) using 6 g of total RNA from each pooled sample. With the polyA-enriched RNA as input, libraries were generated with the Ion Total RNA-Seq Kit v2 (Ion torrent, Life Technologies) and barcoded following the manufacturers instructions, except that the final bead-based size selection was adjusted to favor longer fragments (average read lengths >150 bp).
ENA-FIRST-PUBLIC
2016-03-15T17:02:24Z
ENA-LAST-UPDATE
2018-03-09T09:23:37Z
External Id
SAMEA3638988
INSDC center name
Innsbruck medical University
INSDC first public
2016-03-15T17:02:24Z
INSDC last update
2018-03-09T09:23:37Z
INSDC status
public
Submitter Id
E-MTAB-3993:Sample 9 MSA_SN
biopsy site
substantia nigra
broker name
ArrayExpress
common name
house mouse
disease
multiple system atrophy
genotype
human wild-type alpha synuclein expression in oligodendrocytes
organism part
brain
sample name
E-MTAB-3993:Sample 9 MSA_SN
scientific_name
Mus musculus
strain
Tg(Plp1-SNCA)1Haa