Generic sample from mouse
Mus musculus
Sall1-/-/Sall4-/- ESCs were maintained on mitomycin-C treated feeder cells in ESC complete medium containing DMEM supplemented with 15% FBS (HyClone), 1¡Á non-essential amino acids (Life Technologies), 1¡Á ¦Â-mercaptoethanol (Life Technologies), 2 mM L-glutamine (Life Technologies), 1 mM sodium pyruvate (Life Technologies), 1,000 U/ml leukaemia inhibitory factor and 2i compounds (CHIR99021 and PD0325901, 3 and 1 mM, respectively).
label
sall1-sall4-DKO