T4plusP
mixed sample
Protocols: Arsenate tolerant and non-tolerant Holcus lanatus L. Genovars were selected randomly from a screened population, the arsenic uncontaminated Cruickshank Botanic Garden (CBG) and tillers of the plants were grown hydroponically for 2 weeks in 50ml individual centrifuge tubes with base of the tubes covered in tin-foil to block out light, in heated glasshouse at around 18C under supplemental sodium lamps. Plant tillers from arsenic tolerant (T) and non-tolerant (N) plants were grown hydroponically for 2 weeks in 50ml complete Hogland solution (plusP treatment) or 50ml Hogland solution minus Phosphate (minusP treatment). The experiment therefore investigated 4 treatments (TplusP, TminusP, NplusP, NminusP) and was repeated 2 time. Experiment1 was conducted on 4 plants (n=1), Experiment2 was conducted on 16 plants (n=4). dry roots and shoots for each replicate were ground under liquid nitrogen and total RNA was extrected using the Plant Rneasy kit (Qiagen), with additional on-column Dnase treatment. Extracted RNA of each sample was stored at -80C until shipping on dry ice for sequencing Illumina TrueSeq RNA sample preparation
ENA-FIRST-PUBLIC
2013-11-04T17:01:24Z
ENA-LAST-UPDATE
2018-03-08T16:28:58Z
External Id
SAMEA2075852
INSDC center name
Queen's University Belfast
INSDC first public
2013-11-04T17:01:24Z
INSDC last update
2018-03-08T16:28:58Z
INSDC status
public
Submitter Id
E-MTAB-1678:T4plusP
age
14 day
block
2
broker name
ArrayExpress
common name
velvet grass
growth condition
Hogland solution phosphatelus phosphate
individual
T4plusP
phenotype
Arsenic-tolerant
sample name
E-MTAB-1678:T4plusP
scientific_name
Holcus lanatus
specimen with known storage state
fresh specimen
component_organism
Holcus lanatus
component_organism
Holcus lanatus L