Sinorhizobium meliloti FSM-MA methylation detection - FSM-MA-GD-Mt-3
Sinorhizobium meliloti
Sinorhizobium meliloti FSM-MA WT bacteroids of Medicago truncatula whole nodules methylation detection; Sterilized seeds were germinated on Kalys agar in the dark. Seedlings were inoculated with the bacteria, and then transferred to pots containing a perlite:sand mixture. Plants and grown for 34 days in a greenhouse with occasional watering with a 1 g L-1 nutrient solution (PlantProd solution [N-P-K, 0-15-40; Fertil]). At the end of the growth period, nodules were harvested and stored at -80 until further use. Bacteroids were enriched from the nodule material using Percol gradient centrifugation prior to DNA isolation.
strain
FSM-MA
collection_date
missing
isol_growth_condt
missing
ref_biomaterial
missing
feature
missing
material
missing
biome
missing
num_replicons
missing
host
not applicable
geo_loc_name
missing
lat_lon
missing
isolation_source
missing
BioSampleModel
MIGS.ba