Nipponbare-RSS1-SSR
Oryza sativa Japonica Group
Protocols: A leaf punch of approximately 1 centimeter in diameter was collected from plants DNA Leaf tissue from each rice variety was extracted using a Qiagen DNeasy kit per manufacturer's protocols. DNA from each rice variety was added to Promega PCR Master Mix as per manufacturer's protocol for a 50 microliter reaction. Purification of PCR product was obtained by precipitating with a 9:1 ratio of 3M sodium acetate and 1:3 ratio of 100% ethanol. After 20 minute incubation at -20 Celsius, samples were centrifuged for 30 minutes. Ethanol was removed from the sample and the pellet containing purified PCR product was dried. PCR product was resuspended in double distilled water.
ENA-FIRST-PUBLIC
2017-12-31T17:02:36Z
ENA-LAST-UPDATE
2017-03-15T13:58:50Z
External Id
SAMEA103913906
INSDC center name
OREGON STATE UNIVERS
INSDC first public
2017-12-31T17:02:36Z
INSDC last update
2017-03-15T13:58:50Z
INSDC status
public
Submitter Id
E-MTAB-5583:Nipponbare-RSS1-SSR
broker name
ArrayExpress
common name
Japanese rice
organism part
leaf
sample name
E-MTAB-5583:Nipponbare-RSS1-SSR
scientific_name
Oryza sativa Japonica Group
strain
Nipponbare GSOR: 1000