MAFB sample 1
Homo sapiens
Protocols: Chromatin was prepared from EndoC-βH2 cells by cross-linking cells for 10 minutes with 1% formaldehyde. EndoC-βH2 cells were grown in DMEM containing 5.6 mM glucose, 2% BSA, 50 μM 2-mercaptoethanol, 10 mM nicotinamide, 5.5 μg/mL transferrin, 6.7 ng/mL selenite, 100 units/mL penicillin, and 100 units/mL streptomycin (Scharfmann et al., 2014). Cells were collected and lysed in SDS buffer (Millipore Chromatin Immunoprecipitation Assay Kit. Cat#17-295), and chromatin was sonicated using a Diagenode Bioruptor. Standard DNA sequencing library for sequencing on Illumina sequencing platforms.
ENA-FIRST-PUBLIC
2020-02-01T04:03:05Z
ENA-LAST-UPDATE
2019-12-18T14:22:09Z
External Id
SAMEA6428045
INSDC center name
VANDERBILT UNIVERSITY
INSDC first public
2020-02-01T04:03:05Z
INSDC last update
2019-12-18T14:22:09Z
INSDC status
public
Submitter Id
E-MTAB-8612:MAFB sample 1
broker name
ArrayExpress
cell line
EndoC-betaH2
cell type
type B pancreatic cell
common name
human
developmental stage
embryo
genotype
wild type genotype
organism part
pancreas
sample name
E-MTAB-8612:MAFB sample 1
scientific_name
Homo sapiens