TARA_20130827T1140Z_189_EVENT_PUMP_DCM_(20m)_SEQ-(100L-or-15min)_W>0.8_N010000023
marine metagenome
This sample (TARA_N010000023) was collected at station TARA_189 during campaign TARA_20130801Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130827T1140Z_189_EVENT_PUMP) occurred at position latitudeN=77.9668 and longitudeE=116.6556, on date/time=2013-08-27T11:40:00, at a depth of 18-22 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (>0.8 micrometres) and prepared on board using protocol [SEQ-(100L-or-15min)_W>0.8] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000021, TARA_N010000022, TARA_N010000024. The sample material was split into several aliquot samples including this one and sample(s): TARA_N010000024.
Chlorophyll Sensor
2.13888
Citation
Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)
ENA first public
2019-09-13
ENA last update
2016-08-25
Event Date/Time End
2013-08-27T13:11:00
External Id
SAMEA4397709
INSDC center alias
GSC
INSDC center name
Genoscope
INSDC first public
2019-09-13T17:03:58Z
INSDC last update
2016-08-25T12:05:24Z
INSDC status
public
Latitude End
78.0015
Longitude End
116.4698
Salinity Sensor
32.741495
Submitter Id
TARA_N010000023
depth
20
environment (biome)
Polar Biome
environment (feature)
[DCM] deep chlorophyll maximum layer (ENVO:01000326)
environment (feature) further details
discrete depth within the epipelagic zone (ENVO:00000209) within the photic zone (ENVO:00000209), coinciding with a strong peak in chlorophyll fluorescence, within a marine epipelagic wind mixed layer (ENVO:01000061)
environment (material)
water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)
environmental package
water
event date/time start
2013-08-27T11:40:00
event label
TARA_20130827T1140Z_189_EVENT_PUMP
further details
http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20130827Z_189_WETLAB_GENO.pdf
last update date
2016-04-23Z
latitude start
77.9668
longitude start
116.6556
marine region
[AO] Arctic Ocean (MRGID:1906)
nitrate sensor
99999
oxygen sensor
375.0946
project name
Tara Oceans Expedition (2009-2013)
protocol description
In the size fraction W>0.8 micrometres, the sample material was concentrated on 0.8-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:ATTP14250) on top of a Dacron pad mesh. A volume of 100 Litres was filtered in parallel on two 0.8-micrometre-pore-size filters. Each filter was then packaged in a sterile cryogenic tube (5 mL) (2 tubes, each with 1 filter), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The total volume filtered on the two filters is 100 Litres. However, the volume filtered on each filter is unknown, but approximately 50 Litres. The intention of this protocol is to combine the two filters during molecular entities (nucleotides) extraction and sequencing analysis. A total of 2 (2x 2 tubes) replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.
protocol label
SEQ-(100L-or-15min)_W>0.8
sample collection device
high volume peristaltic pump [HVP-PUMP]
sample material label
TARA_189_DCM_0.8->
sample name
TARA_N010000023
sample status
This version can be used to provide data discovery services
sampling campaign
TARA_20130801Z
sampling platform
SV Tara
sampling station
TARA_189
size fraction lower threshold
0.8
size fraction upper threshold
>
temperature
-0.67976