Sample 29
Homo sapiens
Protocols: Single cells were randomly picked by mouth pipetting with a finely pulled glass capillary and placed directly into lysis buffer containing 1 uL of RNase inhibitor and 19 uL of 0.2% (vol/vol) Triton X-100 solution (Sigma-Aldrich, Germany). The 8-strip PCR tubes containing cells were immediately frozen on dry ice and kept at -80C for downstream applications. Regarding spike-ins: 0.1uL of a 4*10^-6 dilution of the ERCC RNA spike-in mix 1 (Ambion) was added to each sample. na Picelli et al, Full-length RNA-seq from single cells using Smart-seq2, Nature Protocols, 2014
ENA-FIRST-PUBLIC
2020-01-30T04:05:09Z
ENA-LAST-UPDATE
2019-10-04T11:15:13Z
External Id
SAMEA5996529
INSDC center name
Division of Obstetrics and Gynecology Department of Clinical Science, Intervention and Technology Karolinska Institutet, Stockholm, Sweden
INSDC first public
2020-01-30T04:05:09Z
INSDC last update
2019-10-04T11:15:13Z
INSDC status
public
Submitter Id
E-MTAB-8403:Sample 29
age
36, 37
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
normal
organism part
ovary
phenotype
DDX4 positive
post analysis well quality
pass
sample name
E-MTAB-8403:Sample 29
scientific_name
Homo sapiens
sex
female
single cell well quality
OK