TARA_20131027T1016Z_210_EVENT_PUMP_SRF_(5m)_SEQ-(10-100L-or-15min)_W3-20_N010000957
marine metagenome
This sample (TARA_N010000957) was collected at station TARA_210 during campaign TARA_20131020Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20131027T1016Z_210_EVENT_PUMP) occurred at position latitudeN=61.5427 and longitudeE=-55.9869, on date/time=2013-10-27T10:16:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (3-20 micrometres) and prepared on board using protocol [SEQ-(10-100L-or-15min)_W3-20] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists) and attached prokaryotes. This sample has replicate sample(s): TARA_N010000958, TARA_N010000959, TARA_N010000960, TARA_N010000961.
Chlorophyll Sensor
0.99555
Citation
Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)
ENA first public
2019-09-13
ENA last update
2016-08-25
Event Date/Time End
2013-10-27T13:26:00
External Id
SAMEA4398376
INSDC center alias
GSC
INSDC center name
Genoscope
INSDC first public
2019-09-13T17:03:58Z
INSDC last update
2016-08-25T12:24:55Z
INSDC status
public
Latitude End
61.5214
Longitude End
-55.9973
Salinity Sensor
34.212612
Submitter Id
TARA_N010000957
depth
5
environment (biome)
Polar Biome
environment (feature)
[SRF] surface water layer (ENVO:00010504)
environment (feature) further details
discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <50 m from a strong peak in chlorophyll fluorescence
environment (material)
water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)
environmental package
water
event date/time start
2013-10-27T10:16:00
event label
TARA_20131027T1016Z_210_EVENT_PUMP
further details
http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20131027Z_210_WETLAB_GENO.pdf
last update date
2016-04-23Z
latitude start
61.5427
longitude start
-55.9869
marine region
[AO] Arctic Ocean (MRGID:1906)
nitrate sensor
8.291587
oxygen sensor
293.88825
project name
Tara Oceans Expedition (2009-2013)
protocol description
In the size fraction W3-20 micrometres, the sample material was pre-filtered on 20-micrometres, using a nylon mesh, and concentrated on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250). A volume of 10-100 Litres was filtered and the 3-micrometre-pore-size filter was packaged in a sterile cryogenic tube (5 mL), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The volume filtered is indicated for each replicate in the Sample registry (http://doi.pangaea.de/10.1594/PANGAEA.842197). A total of 2-6 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.
protocol label
SEQ-(10-100L-or-15min)_W3-20
sample collection device
high volume peristaltic pump [HVP-PUMP]
sample material label
TARA_210_SRF_3-20
sample name
TARA_N010000957
sample status
This version can be used to provide data discovery services
sampling campaign
TARA_20131020Z
sampling platform
SV Tara
sampling station
TARA_210
size fraction lower threshold
3
size fraction upper threshold
20
temperature
5.2939