A549_dex_4C-seq_Rep1
Homo sapiens
Protocols: Cells were cultured to confluency and treated for 90 min with 1μM dexamethasone. 4C experiments were performed as previously described (van de Werken et al., Methods Enzymol, 2012. 513: p. 89-112.), using 5x10^6 cells. 4-bp cutters were used as primary (CviQI, Thermo Fisher Scientific) and secondary (DpnII, Thermo Fisher Scientific) restriction enzymes. For each cell line 4C libraries were generated in two biological replicates. Based on 4C-libraries we amplified a total of 1.2 μg of DNA by inverse-PCR using viewpoint-specific primers (fwd: CTACACGACGCTCTTCCGATCTAATGTTCAGGTGTGGGAGTAC, rev: CAGACGTGTGCTCTTCCGATCTGCCCTCTGCCTCTTGTTAGG), which include adaptors for subsequent high throughput sequencing (Illumina HiSeq2500).
ENA first public
2017-12-31
ENA last update
2016-12-19
External Id
SAMEA30153418
INSDC center alias
Abt Vingron Max Planck Institute for Molecular Genetics
INSDC center name
Abt Vingron Max Planck Institute for Molecular Genetics
INSDC first public
2017-12-31T17:02:35Z
INSDC last update
2016-12-19T17:53:50Z
INSDC status
public
Submitter Id
E-MTAB-5350:A549_dex_4C-seq_Rep1
broker name
ArrayExpress
cell line
A549
common name
human
genotype
wild type genotype
sample name
E-MTAB-5350:A549_dex_4C-seq_Rep1
stimulus
dexamethasone