A
Homo sapiens
Protocols: HAP1 cells were derived from the near-haploid chronic myeloid leukaemia cell line KBM7 as described (Carette et al., 2011). HAP1 cells were cultured in IMDM supplemented with 10% FBS, penicillin-streptomycin and L-glutamine. Cells were incubated at 37°C in 5% CO2 and grown to 80-90% confluence before harvesting total RNA Total RNA from 5 million cells was extracted using Qiagen RNeasy plus mini kit (74134). 1 μg of total RNA was subjected to library synthesis using the TruSeq RNA-Seq kit in technical replicate (e.g. two libraries were made from each RNA sample). 1 μg of total RNA was enriched for Poly-A mRNA using oligo dT-selection and reverse transcribed to cDNA. The cDNA was ligated with Illumina indexes. RNA libraries were prepared for sequencing using standard Illumina protocols.
ENA first public
2017-07-25
ENA last update
2017-07-11
External Id
SAMEA104158618
INSDC center alias
Stanford University
INSDC center name
Stanford University
INSDC first public
2017-07-25T17:01:20Z
INSDC last update
2017-07-11T12:18:43Z
INSDC status
public
Submitter Id
E-MTAB-5865:A
broker name
ArrayExpress
cell line
HAP-1
cell type
haploid cell
common name
human
disease
chronic myelogenous leukemia
genotype
wild type genotype
sample name
E-MTAB-5865:A