HX_f_38_18_NEV_4_mw
marine metagenome
coastal Aegean Sea (Eastern Mediterranean); plankton; eukaryotic and prokaryotic communities composition; amplicons; illumina MiSeq
DNA extraction method
PowerWater DNA isolation kit
ENA-FIRST-PUBLIC
2019-06-24T04:04:00Z
ENA-LAST-UPDATE
2018-04-25T08:57:32Z
External Id
SAMEA4609289
INSDC center name
HELLENIC CENTRE FOR MARINE RESEARCH
INSDC first public
2019-06-24T04:04:00Z
INSDC last update
2018-04-25T08:57:32Z
INSDC status
public
Latitude End
38.50766667
Longitude End
23.517
Submitter Id
HX_f_38_18
amount or size of sample collected
2
depth
10
environment (biome)
temperate mediterannean sea biome
environment (feature)
marine pelagic feature
environment (material)
planktonic material
environmental package
water
event date/time
2014-07-22T15:00:00Z
fraction_microns
0.2
latitude start
38.50766667
longitude start
23.517
marine region
Aegean Sea (eastern Mediterranean)
primer_f
5'CCCTGCCHTTTGTACACAC
primer_f_reference
Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372.
primer_r
5'CCTTCYGCAGGTTCACCTAC
primer_r_reference
Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLoS One 4: e6372.
project name
HX_field
protocol label
filtration and preservation
salinity
37.5911
sample collection device or method
filtration using peristaltic pump
sample name
HX_f_38_18
sample storage duration
20
sample storage temperature
-80
sampling campaign
cruise
sampling platform
Research Vessel HCMR
sampling site
HX_f_NEV4
scientific_name
marine metagenome
target gene
18S
temperature
26.18