DBal_RNA-seq_F1CyOTM3_heads_Rep1
Drosophila melanogaster
Protocols: Male F1 (+/CyO; +/TM3) and female virginizer flies were crossed in vials. Adults (at least 20) from the F1 generation with the (+/CyO; +/TM3) phenotype were selected, their heads cut and snap-frozen in liquid nitrogen. Two independent collections were performed. Fly heads or embryos were homogenised in TRIzol LS (Life Technologies) with a Cordless Motor for Pellet Mix and pestels (VWR) on ice. RNA was extracted according to the manufacturer???s instructions, and the remaining DNA digested with RNase-free DNase I (Roche) for 30min. The solution was finally purified a second time using Agencourt RNAClean XP beads (Beckman Coulter). Libraries were prepared following instructions of the NEBNext ultra directional RNA library prep kit for Illumina sequencing (NEB).
ENA-FIRST-PUBLIC
2019-04-24T04:04:22Z
ENA-LAST-UPDATE
2018-12-10T13:16:06Z
External Id
SAMEA5160819
INSDC center name
EMBL
INSDC first public
2019-04-24T04:04:22Z
INSDC last update
2018-12-10T13:16:06Z
INSDC status
public
Submitter Id
E-MTAB-7511:DBal_RNA-seq_F1CyOTM3_heads_Rep1
broker name
ArrayExpress
common name
fruit fly
developmental stage
adult
genotype
+/CyO; +/TM3
organism part
head
sample name
E-MTAB-7511:DBal_RNA-seq_F1CyOTM3_heads_Rep1
scientific_name
Drosophila melanogaster
sex
mixed sex
strain
F1CyOTM3