Fib.C
Homo sapiens
Protocols: Newborn skin (FibA, B) and fetal lung (FibC) fibroblasts were acquired from ATCC (PCS-201-010, CRL-2097 and CCL-15, respectively). Fibroblasts derived from three PPMS diseased individuals were kindly provided by Gianvito Martino. Fibroblasts derived from 20 year old male PMD (643C>T) patient were acquired from Coriell Institute (ID:GM0954). Fibroblasts derived from healthy adult male individual were used as control for PMD cells. The study was approved by the local ethical committees in Münster and Milan (AZ 2018-040-f-S). Fibroblasts were cultured in fibroblast medium (FM): Dulbecco's Modified Eagle Medium high glucose (Sigma) containing 10% FBS (Gibco), 1% nonessential amino acids (NEAA) (Sigma), 1% GlutaMAX (Gibco), and 1% penicillin/streptomycin solution (Sigma). All cell lines were negative for HIV, HCV, HBV and mycoplasma contamination. Following total RNA extraction using the RNasy Mini Kit (Quiagen) and DNase (Quiagen) treatment the quality of the produced RNA was tested with Bioanalyzer analysis (Agilent). Samples with RIN value over 7 were used for RNA sequencing. The date of RNA Isolation is 06.09.2018. NEBNext® Poly(A) mRNA Magnetic Isolation Module was followed by a directional library preparation (NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®). The date of library preparation is 17.09.2018.
ENA-FIRST-PUBLIC
2020-01-31T04:02:42Z
ENA-LAST-UPDATE
2019-04-02T16:09:32Z
External Id
SAMEA5546672
INSDC center name
Max Planck Institute for Molecular Biomedicine
INSDC first public
2020-01-31T04:02:42Z
INSDC last update
2019-04-02T16:09:32Z
INSDC status
public
Submitter Id
E-MTAB-7838:Fib.C
broker name
ArrayExpress
catalog number
CCL-153
cell line
lung fibroblast derived cell line
cell type
fetus lung fibroblasts
common name
human
developmental stage
fetus
individual
Fib.C
organism part
lung fibroblast
progenitor cell type
lung fibroblast
sample name
E-MTAB-7838:Fib.C
scientific_name
Homo sapiens
sex
male