Sample 7
Homo sapiens
Protocols: Methodology: U2OS cells were infected with retrovirus expressing NUAK1 shRNA4977 (see Liu et al. Nature. 2012 for shRNA details) or non-targeting control shRNA. 24hrs after infected, cells were selected on Puromycin for 48hrs, then trypsinized and re-seeded. Cells were harvested 24hrs after re-seeding and RNA was isolated using Qiagen RNEasy midi kits. cDNA was generated using TruSeq Stranded mRNA Library Prep Kit (Illumina). Following library quantification (D1000 ScreenTape, Agilent Technologies), libraries were standardized to 10nM, denatured, diluted to 10pM and analyzed by paired-end sequencing using an Illumina NextSeq500 platform. Sample number NM 1 to 3 are treated with shCtrl. Sample number NM7 to 9 are shNUAK1. Cells were lysed directly on 10cm dishes using Qiagen RNEasy extraction buffer RNA was isolated using Qiagen RNEasy cDNA was synthesized with the TruSeq Stranded mRNA Library Prep Kit (Illumina)
ENA first public
2018-01-01
ENA last update
2017-11-24
External Id
SAMEA104402385
INSDC center alias
BAH-GU
INSDC center name
University of Glasgow
INSDC first public
2018-01-01T17:01:44Z
INSDC last update
2017-11-24T13:53:16Z
INSDC status
public
Submitter Id
E-MTAB-6244:Sample 7
broker name
ArrayExpress
cell line
U2OS
cell type
epithelial cell
common name
human
disease
osteosarcoma
organism part
bone tissue
sample name
E-MTAB-6244:Sample 7