PBX1_si389_2
Homo sapiens
Protocols: 697 cells (described here: https://www.dsmz.de/collection/catalogue/details/culture/ACC-42) were infected with shRNA lentivirus with 8 ug/mL polybrene in complete medium. The medium was replaced after 12 h of infection for the cells to recover for 36 h prior to 2 µg/mL puromycin selection for 48 h before harvesting the cells for RNA extraction. The shRNA used for knockdown are purchased from Dharmacon. Clone ID TRCN0000020389 and TRCN0000020392 were used for PBX1 knockdown. Clone ID TRCN0000017534 and TRCN0000017535 were used for E2A knockdown. 697 cells were cultured in RPMI 1640 (Invitrogen) and 10% FBS. RNA was extracted with RNeasy Mini Kit (Qiagen). Libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina).
ENA-FIRST-PUBLIC
2020-05-13T17:05:14Z
ENA-LAST-UPDATE
2019-07-24T10:20:52Z
External Id
SAMEA5807716
INSDC center name
TMU Research Center of Cancer Translational Medicine, Taipei Medical University
INSDC first public
2020-05-13T17:05:14Z
INSDC last update
2019-07-24T10:20:52Z
INSDC status
public
Submitter Id
E-MTAB-8179:PBX1_si389_2
broker name
ArrayExpress
cell line
697
cell type
pre-B cell
common name
human
disease
B-cell acute lymphoblastic leukemia
genotype
wild type genotype
organism part
bone marrow
sample name
E-MTAB-8179:PBX1_si389_2
scientific_name
Homo sapiens