TARA_20130702T0405Z_168_EVENT_NET_MIX_(0-100m)_SEQ-(500mL-or-15min)_N20-180_N010000531
marine metagenome
This sample (TARA_N010000531) was collected at station TARA_168 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-DOUBLE-20]+[SIEVE-180]. The sampling event (TARA_20130702T0405Z_168_EVENT_NET) occurred at position latitudeN=72.5144 and longitudeE=44.0528, on date/time=2013-07-02T04:05:00, at a depth of 0-100 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [MIX] marine epipelagic wind mixed layer (ENVO:01000061). The sample material was size-fractionated (20-180 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N20-180] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000532, TARA_N010000533, TARA_N010000534.
Chlorophyll Sensor
0.2268
Citation
Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)
ENA first public
2019-09-13
ENA last update
2016-08-25
Event Date/Time End
2013-07-02T04:16:00
External Id
SAMEA4397190
INSDC center alias
GSC
INSDC center name
Genoscope
INSDC first public
2019-09-13T17:03:57Z
INSDC last update
2016-08-25T11:48:19Z
INSDC status
public
Latitude End
72.5175
Longitude End
44.0545
Salinity Sensor
34.97715
Submitter Id
TARA_N010000531
depth
0-100
environment (biome)
Polar Biome
environment (feature)
[MIX] marine epipelagic wind mixed layer (ENVO:01000061)
environment (feature) further details
depth interval within the epipelagic zone (ENVO:00000209) including the photic zone (ENVO:00000209) and part of the aphotic zone (ENVO:00000210), including a weak nutricline (ENVO:01000260), with a difference in nitrate concentrations of 5-10 micromoles per kilogramme between 10 m and the depth of the nutricline
environment (material)
particulate matter (ENVO:01000060), including planktonic material (ENVO:01000063)
environmental package
water
event date/time start
2013-07-02T04:05:00
event label
TARA_20130702T0405Z_168_EVENT_NET
further details
http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20130701Z_168_WETLAB_GENO.pdf
last update date
2016-04-23Z
latitude start
72.5144
longitude start
44.0528
marine region
[AO] Arctic Ocean (MRGID:1906)
nitrate sensor
-0.994306
oxygen sensor
302.4059
project name
Tara Oceans Expedition (2009-2013)
protocol description
In the size fraction N20-180 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 20-micrometres and passed through a sieve with a metal mesh of 180-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.
protocol label
SEQ-(500mL-or-15min)_N20-180
sample collection device
plankton net [NET-DOUBLE-20]+[SIEVE-180]
sample material label
TARA_168_MIX_20-180
sample name
TARA_N010000531
sample status
This version can be used to provide data discovery services
sampling campaign
TARA_20130629Z
sampling platform
SV Tara
sampling station
TARA_168
size fraction lower threshold
20
size fraction upper threshold
180
temperature
3.25548