grxs17-1_R1
Arabidopsis thaliana
Protocols: Seeds were surface-sterilized and sown in MS-agar medium and stratified at 4°C in darkness for 3 days, then germinated for one week under long days (16h light/8h dark) at 21°C. RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega) preparation of strand-specific cDNA libraries: Purification of poly-A containing mRNA molecules; mRNA fragmentation; Random primed cDNA synthesis (strand specific); Adapter ligation and adapter specific PCR amplification.
ENA first public
2018-01-02
ENA last update
2016-08-10
External Id
SAMEA4378188
INSDC center alias
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
INSDC center name
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
INSDC first public
2018-01-02T17:02:30Z
INSDC last update
2016-08-10T17:21:10Z
INSDC status
public
Submitter Id
E-MTAB-5001:grxs17-1_R1
broker name
ArrayExpress
common name
thale cress
developmental stage
Seedling
ecotype
Columbia
genetic modification
SALK_021301
replicate
R1
sample name
E-MTAB-5001:grxs17-1_R1