P. micropora RNA
Paulinella micropora
P. micropora MYN1 strain was cultured under 14 hours light (30-40 microE m-2s-1) / 10 hours dark photoperiodic cycle at 25 degree C in the modified Waris-H+Si medium. The cells at the time points of 0, 4, 8, 12, 16, and 20 hours since the start of light-phase were harvested by low-speed centrifugation (500 x g, 2 minutes, 4 degree C), and then they were passed through 20 micrometer mesh-filter (CellTrics, Sysmex Partec GmbH, Gorlitz, Germany) to eliminate contaminating bacteria that were enriched in the dead P. micropora cell aggregates larger than 20 micrometer. After washing the cells with fresh culture medium, the RNAs were extracted using Trizol reagent (Life technologies). Equal amounts of the RNA samples acquired from each time-point's cells were mixed and the mixtures were used in RNA-seq, Iso-Seq and TSS-seq analysis.
sample_name
P. micropora RNA
biomaterial_provider
Mami Nomura, Ken-ichiro Ishida, Graduate School of Life and Environmental Science, University of Tsukuba
collection_date
2009-10-09
geo_loc_name
Japan:Ibaraki, Tsukuba
isolation_source
pond
strain
MYN1