mhpT_2
Escherichia coli str. K-12 substr. MG1655
Protocols: Cell cultures centrifuged and frozen. Overnights grown in LB 0.2% glucose, 100ug/mL ampicillin, 30ug/mL chloramphenicol, washed and subcultured with 0.2% arabinose (induction), no selection. Colony PCR performed from frozen samples, amplifying CRISPR array expansion. Oligonucleotides JW7815 and JW7816 amplify the CRISPR I array expansion. Oligonucleotides JW7817 and JW7818 amplify the CRISPR II array expansion. PCR product containing expanded array were gel purified. Gel purified PCR products were used as a template in second PCR, where the oligonucleotides contain barcoded adapters
ENA-FIRST-PUBLIC
2020-01-30T04:03:47Z
ENA-LAST-UPDATE
2020-01-20T10:43:41Z
External Id
SAMEA6491870
INSDC center name
The Wadsworth Center, NYSDOH
INSDC first public
2020-01-30T04:03:47Z
INSDC last update
2020-01-20T10:43:41Z
INSDC status
public
Submitter Id
E-MTAB-8700:mhpT_2
array sequenced
CRISPR II
broker name
ArrayExpress
genotype
MG1655 cas3::PcseI + pBAD33-wtcas3
growth condition
LB + 0.2% arabinose, 1 hr induction
sample name
E-MTAB-8700:mhpT_2
scientific_name
Escherichia coli str. K-12 substr. MG1655
spacer plasmid
pAMD212
target
10 kb ds lacZ promoter (mhpT)
strain
K-12
substrain
MG1655