S06
Mus musculus
Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina)
ENA-FIRST-PUBLIC
2020-02-01T04:03:03Z
ENA-LAST-UPDATE
2019-11-07T17:25:26Z
External Id
SAMEA6163210
INSDC center name
UZH
INSDC first public
2020-02-01T04:03:03Z
INSDC last update
2019-11-07T17:25:26Z
INSDC status
public
Submitter Id
E-MTAB-8500:S06
age
12
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
3
organism part
colon
sample name
E-MTAB-8500:S06
sampling site
distal colon
scientific_name
Mus musculus
sex
female
strain
C57BL/6J