Posterior Area Opaca
Gallus gallus
Protocols: Prestreak XII-XIII embryos (Eyal-Giladi and Kochav's stages described here DOI: 10.1016/0012-1606(76)90178-0) were harvested either into ice cold Ca2+Mg2+-free PBS (CMF-PBS) containing 0.2mM EDTA for tissue separation, or into ice-cold Tyrode's saline for tissue dissection. To separate the endocardium from ventricular tissue, 10µg/ml hyaluronidase from bovine testes was used. HH12 hearts were separated from surrounding splanchnic mesoderm, foregut and anterior-intestinal portal (AIP) endoderm. Dissected tissues were collected and pooled in 200ul of ice-cold RNA stabilisation solution, RNAlater and stored at 4°C. RNA was isolated using RNAqueous Micro-kit according to the manufacturer's instructions. DNA was removed by applying DNase treatment. The RNA quality was measured by using Agilent 2200 TapeStation Libraries were constructed from 100ng total RNA using the NEBNext® Ultra™ II Directional RNA Library Prep Kit with NEBNext® Poly(A) mRNA Magnetic Isolation Module according to the manufacturer's instructions. Libraries to be multiplexed in the same run were pooled in equimolar quantities
ENA-FIRST-PUBLIC
2020-01-31T04:02:43Z
ENA-LAST-UPDATE
2019-05-08T11:59:25Z
External Id
SAMEA5597700
INSDC center name
Cell and Developmental Biology, University College London
INSDC first public
2020-01-31T04:02:43Z
INSDC last update
2019-05-08T11:59:25Z
INSDC status
public
Submitter Id
E-MTAB-7941:Posterior Area Opaca
age
1 to 6
breed
White Leghorn
broker name
ArrayExpress
common name
chicken
developmental stage
Eyal-Giladi and Kochav's stage XII to XIII
individual
mixed pool of embryos
organism part
area opaca
sample name
E-MTAB-7941:Posterior Area Opaca
sampling site
posterior
scientific_name
Gallus gallus