WT_2
Mus musculus
Protocols: Peripheral lymph nodes (LN) were minced and digested at 37 degrees C in RPMI (PAA) containing 0.25 mg/ml Collagenase D (Roche), 1 U/ml Dispase I (Roche), 25 mM Hepes, pH 7.2, and 120 U/ml DNase I (Roche) for 40 min, followed by addition of 5 mM EDTA and incubation for 5 min. LNs were digested with 0.5U/ml Liberase TM (Roche) and DNase I. Aire-ILC3s were sorted as Lin-, MHCII+, CD80+, IL7Ra+ cells on INFLUX sorter (BD). Total mRNA was extracted from the FACS-sorted cells using RNeasy Plus Microkit (Qiagen). The quality of the isolated RNA was controlled by Bioanalyzer 2100 electrophoresis (Agilent). RNA-seq libraries were prepared by EMBL Gene core facility in Heidelberg.
ENA-FIRST-PUBLIC
2019-03-14T17:02:42Z
ENA-LAST-UPDATE
2018-07-27T11:51:35Z
External Id
SAMEA4807022
INSDC center name
Laboratory of Genomics and Bioinformatics Institute of Molecular Genetics of the ASCR
INSDC first public
2019-03-14T17:02:42Z
INSDC last update
2018-07-27T11:51:35Z
INSDC status
public
Submitter Id
E-MTAB-7088:WT_2
age
4 to 6
broker name
ArrayExpress
cell type
group 3 innate lymphoid cell
common name
house mouse
genotype
wild type genotype
organism part
lymph node
phenotype
Lin-, MHCII+, CD80+, IL7Ra+
sample name
E-MTAB-7088:WT_2
scientific_name
Mus musculus
sex
female
strain
BALB/c