Sample 3
Mus musculus
Protocols: Draining inguinal lymph nodes were harvested, collected in PBS and gently smashed across a wire mesh. The dissociated cell suspension was stained with anti-CD19, anti-B220, anti-CD38, anti-GL7 and the viability marker 7AAD in PBS containing 0.5% FCS and 10mM EDTA. VSV immunization protocol:Mice were immunized sub-cutaneously (s.c) with 50 micrograms of NP-KLH in alum on day 0. Germinal center B cells from draining inguinal lymph nodes were examined at day 12 following the NP-KLH immunization. CD19+ CD38- GL7+ cells from lymph nodes of immunized Cxcl13-Cre/TdTomato EYFP or Cxcl13-Cre/TdTomato Cxcl12fl/fl EYFP mice were sorted using the BioRad S3 Cell Sorter or the BD FACS Melody cell sorter. Single cell suspensions were run on the 10x Chromium (10x Genomics) system. The cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3' Reagent Kit (v2 Chemistry). For B cell repertoire analysis, the Chromium Single Cell 5' Library Construction and Mouse V(D)J Enrichment kits (V1.0 Chemistry) were used.
ENA-FIRST-PUBLIC
2020-05-12T17:04:59Z
ENA-LAST-UPDATE
2020-04-07T11:33:08Z
External Id
SAMEA6650829
INSDC center name
Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
INSDC first public
2020-05-12T17:04:59Z
INSDC last update
2020-04-07T11:33:08Z
INSDC status
public
Submitter Id
E-MTAB-8904:Sample 3
age
6 to 8
broker name
ArrayExpress
cell type
germinal center B cell
common name
house mouse
developmental stage
adult
genotype
Tg(Cxcl13-Cre/TdTomato)+ x R26-EYFP++ x Cxcl12fl/fl
immunophenotype
CD19+ CD38- GL7+
organism part
inguinal lymph node
replicate
1
sample name
E-MTAB-8904:Sample 3
scientific_name
Mus musculus
sex
mixed
stimulus
subcutaneous NP-KLH immunization
strain
C57BL/6N