centipede_1
mixed sample
Protocols: Manual collection of specimen from natural habitat (cave), crushed and preserved in liquid RNA-later (Qiagen, USA). Total RNA was extract using TRIzol reagent (Invitrogen, USA) according to the manufacturer's instructions. Messenger RNA (mRNA) was isolated from total RNA using the Dynabeads mRNA Purification Kit (Invitrogen, Carlsbad Ca.USA). The mRNA was fragmented and transcribed into first-strand cDNA using SuperScript(tm) II Reverse Transcriptase (Invitrogen, Carlsbad Ca. USA) and N6 primer (IDT). RNase H (Invitrogen, Carlsbad Ca. USA) and DNA polymerase I (Invitrogen, Shanghai ChinaNew England BioLabs) were subsequently applied to synthesize the second-strand cDNA. The double-stranded cDNA then underwent end-repair, a single 'A' base addition, adapter ligation, and size selection (~250bp), indexed and PCR amplification to construct library.
ENA-FIRST-PUBLIC
2013-10-28T17:01:33Z
ENA-LAST-UPDATE
2018-03-09T10:23:38Z
External Id
SAMEA2177828
INSDC center name
GigaScience
INSDC first public
2013-10-28T17:01:33Z
INSDC last update
2018-03-09T10:23:38Z
INSDC status
public
Submitter Id
E-MTAB-1859:centipede_1
broker name
ArrayExpress
life stage
adult
sample amount
x1 centipede
sample name
E-MTAB-1859:centipede_1
scientific_name
Eupolybothrus cavernicolus
sex
male
specimen with known storage state
fresh specimen
component_organism
Eupolybothrus cavernicolus
component_organism
Eupolybothrus cavernicolus