liver_CAG_5
Mus musculus
Protocols: Adult animals requiring 4-OHT were i.p. injected with 1 mg (Z)-4-hydroxytamoxifen (4-OHT; Sigma, H7904) in 10 % ethanol and vegetable oil (5 mg/ml) and collected 4 hours post injection. Mouse tissue was harvested from mice directly into liquid nitrogen. Total RNA from tissues was purified using TRIzol (Invitrogen). Briefly, tissue was homogenized in Trizol (Invitrogen) and chloroform was added. After centrifugation, RNA was purified using RNeasy Mini columns (Qiagen) according to the manufacturer's protocol, including the on-column DNase I digestion step. 5 µg of purified RNA was treated with Ribozero rRNA removal kit (Illumina) and ethanol precipitated. RNA quality and removal of rRNA were checked with the Agilent 2100 Bioanalyser (Agilent Technologies). Libraries for RNA-seq were then prepared with the TruSeq RNA Sample Prep Kits v2 (Illumina)
ENA-FIRST-PUBLIC
2020-01-31T04:02:40Z
ENA-LAST-UPDATE
2019-02-01T11:54:20Z
External Id
SAMEA5314248
INSDC center name
UNIVERSITY OF CAMBRIDGE
INSDC first public
2020-01-31T04:02:40Z
INSDC last update
2019-02-01T11:54:20Z
INSDC status
public
Submitter Id
E-MTAB-7636:liver_CAG_5
age
8
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
R26CAG-c-MycERT2/+
individual
5
organism part
liver
sample name
E-MTAB-7636:liver_CAG_5
scientific_name
Mus musculus
sex
female
strain
mixed