TARA_20130718T0305Z_180_EVENT_NET_SRF_(5m)_SEQ-(500mL-or-15min)_N180-2000_N010000895
marine metagenome
This sample (TARA_N010000895) was collected at station TARA_180 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a plankton net [NET-BONGO-180]+[SIEVE-2000]. The sampling event (TARA_20130718T0305Z_180_EVENT_NET) occurred at position latitudeN=74.8063 and longitudeE=76.1759, on date/time=2013-07-18T03:05:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (180-2000 micrometres) and prepared on board using protocol [SEQ-(500mL-or-15min)_N180-2000] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000893, TARA_N010000894, TARA_N010000896.
Chlorophyll Sensor
0.463462
Citation
Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)
ENA first public
2019-09-13
ENA last update
2016-08-25
Event Date/Time End
2013-07-18T03:15:00
External Id
SAMEA4397500
INSDC center alias
GSC
INSDC center name
Genoscope
INSDC first public
2019-09-13T17:03:57Z
INSDC last update
2016-08-25T11:55:20Z
INSDC status
public
Latitude End
74.8113
Longitude End
76.1953
Salinity Sensor
22.047506
Submitter Id
TARA_N010000895
depth
5
environment (biome)
Polar Biome
environment (feature)
[SRF] surface water layer (ENVO:00010504)
environment (feature) further details
discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <50 m from a strong peak in chlorophyll fluorescence, within <50 m from the sea floor (ENVO:00000482)
environment (material)
particulate matter (ENVO:01000060), including planktonic material (ENVO:01000063)
environmental package
water
event date/time start
2013-07-18T03:05:00
event label
TARA_20130718T0305Z_180_EVENT_NET
further details
http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20130718Z_180_WETLAB_GENO.pdf
last update date
2016-04-23Z
latitude start
74.8063
longitude start
76.1759
marine region
[AO] Arctic Ocean (MRGID:1906)
nitrate sensor
99999
oxygen sensor
350.63975
project name
Tara Oceans Expedition (2009-2013)
protocol description
In the size fraction N180-2000 micrometres, the sample material was obtained from a net-cod-end collector lined with a nylon mesh of 180-micrometres and passed through a sieve with a metal mesh of 2000-micrometres. Filtered sea water (<0.1-micrometre) was added to the filtrate up to a total volume of 3 Litres. A volume of 500 mL from the diluted filtrate was filtered in parallel on two 10-micrometres-pore-size, 47-mm-diameter Millipore polycarbonate membrane filters (ref:TCTP04700), packaged in a sterile cryogenic tube (5 mL) (2 filters in 1 tube), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.
protocol label
SEQ-(500mL-or-15min)_N180-2000
sample collection device
plankton net [NET-BONGO-180]+[SIEVE-2000]
sample material label
TARA_180_SRF_180-2000
sample name
TARA_N010000895
sample status
This version can be used to provide data discovery services
sampling campaign
TARA_20130629Z
sampling platform
SV Tara
sampling station
TARA_180
size fraction lower threshold
180
size fraction upper threshold
2000
temperature
3.480781