DBal_RNA-seq_N1mat_6-8h_Rep1
Drosophila melanogaster
Protocols: Female F1 (+/CyO; +/TM3) and male virginizer flies were crossed in population cages at 25 degr. After three one-hour prelays, embryos were collected at 6-8 h after egg lay. Two independent collections were performed. The collected embryos were dechorionated using 50% bleach. Unfertilized eggs were discarded by rapid hand-sorting under the microscope. Properly staged enbryos were snap-frozen in liquid nitrogen. Fly heads or embryos were homogenised in TRIzol LS (Life Technologies) with a Cordless Motor for Pellet Mix and pestels (VWR) on ice. RNA was extracted according to the manufacturer???s instructions, and the remaining DNA digested with RNase-free DNase I (Roche) for 30min. The solution was finally purified a second time using Agencourt RNAClean XP beads (Beckman Coulter). Libraries were prepared following instructions of the NEBNext ultra directional RNA library prep kit for Illumina sequencing (NEB).
ENA-FIRST-PUBLIC
2019-04-24T04:04:22Z
ENA-LAST-UPDATE
2018-12-10T13:16:06Z
External Id
SAMEA5160825
INSDC center name
EMBL
INSDC first public
2019-04-24T04:04:22Z
INSDC last update
2018-12-10T13:16:06Z
INSDC status
public
Submitter Id
E-MTAB-7511:DBal_RNA-seq_N1mat_6-8h_Rep1
age
6 to 8
broker name
ArrayExpress
common name
fruit fly
developmental stage
stages 10-11
genotype
+/+; +/+ || +/CyO; +/TM3 || +/CyO; +/+ || +/+; +/TM3
initial time point
egg laying
organism part
whole embryo
sample name
E-MTAB-7511:DBal_RNA-seq_N1mat_6-8h_Rep1
scientific_name
Drosophila melanogaster
sex
mixed sex
strain
N1mat