TARA_20130710T0358Z_175_EVENT_PUMP_SRF_(5m)_SEQ-(100L-or-15min)_W>0.8_N010000452
marine metagenome
This sample (TARA_N010000452) was collected at station TARA_175 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130710T0358Z_175_EVENT_PUMP) occurred at position latitudeN=79.2233 and longitudeE=66.3435, on date/time=2013-07-10T03:58:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (>0.8 micrometres) and prepared on board using protocol [SEQ-(100L-or-15min)_W>0.8] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity, metagenomics and/or transcriptomics studies of unicellular eukaryotes (protists). This sample has replicate sample(s): TARA_N010000453, TARA_N010000454, TARA_E400007000, TARA_N010000455, TARA_N010000436, TARA_N010000437. The sample material was split into several aliquot samples including this one and sample(s): TARA_N010000453.
Chlorophyll Sensor
0.89622
Citation
Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)
ENA first public
2019-09-13
ENA last update
2016-08-25
Event Date/Time End
2013-07-10T08:20:00
External Id
SAMEA4397315
INSDC center alias
GSC
INSDC center name
Genoscope
INSDC first public
2019-09-13T17:03:57Z
INSDC last update
2016-08-25T11:55:06Z
INSDC status
public
Latitude End
79.2023
Longitude End
66.5078
Salinity Sensor
34.2633
Submitter Id
TARA_N010000452
depth
5
environment (biome)
Polar Biome
environment (feature)
[SRF] surface water layer (ENVO:00010504)
environment (feature) further details
discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <10 m from a strong peak in chlorophyll fluorescence
environment (material)
water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)
environmental package
water
event date/time start
2013-07-10T03:58:00
event label
TARA_20130710T0358Z_175_EVENT_PUMP
further details
http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20130710Z_175_WETLAB_GENO.pdf
last update date
2016-04-23Z
latitude start
79.2233
longitude start
66.3435
marine region
[AO] Arctic Ocean (MRGID:1906)
nitrate sensor
2.060443
oxygen sensor
368.906875
project name
Tara Oceans Expedition (2009-2013)
protocol description
In the size fraction W>0.8 micrometres, the sample material was concentrated on 0.8-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:ATTP14250) on top of a Dacron pad mesh. A volume of 100 Litres was filtered in parallel on two 0.8-micrometre-pore-size filters. Each filter was then packaged in a sterile cryogenic tube (5 mL) (2 tubes, each with 1 filter), labelled with a barcode identification sticker and stored on board in liquid nitrogen. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The entire process did not exceed 15 minutes. Note 4: The total volume filtered on the two filters is 100 Litres. However, the volume filtered on each filter is unknown, but approximately 50 Litres. The intention of this protocol is to combine the two filters during molecular entities (nucleotides) extraction and sequencing analysis. A total of 2 (2x 2 tubes) replicates were prepared and sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.
protocol label
SEQ-(100L-or-15min)_W>0.8
sample collection device
high volume peristaltic pump [HVP-PUMP]
sample material label
TARA_175_SRF_0.8->
sample name
TARA_N010000452
sample status
This version can be used to provide data discovery services
sampling campaign
TARA_20130629Z
sampling platform
SV Tara
sampling station
TARA_175
size fraction lower threshold
0.8
size fraction upper threshold
>
temperature
1.421805