HA-Chaf1a
Mus musculus
source_name
F9 Cells
chip antibody
HA-probe sc-7392 (Santa Cruz Biotechnology)
treatment
CRISPR and donor plasmids were co-transfected into F9 cells. The donor plasmid was cloned by combining two homologous sequences of Chaf1a with 3HA in pCAG containing puromycin resistance.