Run2_DDX4_Ab_neg
Homo sapiens
Protocols: Whole ovaries were received, medulla was trimmed away using scalpels and remaining cortex was cry-preserved in 1x1x1mm pieces using slow-freezing in Ethylene Glycol. After thawing of tissue, pieces were cut into smaller pieces using scalpels and enzymatically digested (Liberase, Collagenase IV, DNase I) on 37C. After digestion into single cells, cells were either prepared for Dead Cell Removal kit (run1) or FACS (run2). Single droplets containing one cell/droplet was prepared from the cell suspension in 0.04% BSA using 10x chromium controller. Nucleic acid extraction was performed per in chip inside 10x chromium controller. 10x Genomics single cell 3' protocol was followed.
ENA-FIRST-PUBLIC
2020-01-30T04:05:10Z
ENA-LAST-UPDATE
2019-10-14T16:35:00Z
External Id
SAMEA6082044
INSDC center name
Division of Obstetrics and Gynecology Department of Clinical Science, Intervention and Technology Karolinska Institutet, Stockholm, Sweden
INSDC first public
2020-01-30T04:05:10Z
INSDC last update
2019-10-14T16:35:00Z
INSDC status
public
Submitter Id
E-MTAB-8381:Run2_DDX4_Ab_neg
age
20 and 22 and 26
broker name
ArrayExpress
common name
human
developmental stage
adult
disease
normal
individual
pool of donors
organism part
ovary
sample name
E-MTAB-8381:Run2_DDX4_Ab_neg
sampling site
ovarian cortex
scientific_name
Homo sapiens
sex
female