Chlamydomonas reinhardtii cc503 cw92 mt+ under carbon deprivation
Chlamydomonas reinhardtii
C. reinhardtii (strain cc503 cw92 mt+) was cultured in 2-L flasks in photoautotrophic and temperature-controlled condition. Cells were cultured in H20P medium (modified TAP medium without acetate, supplemented with 20 mM HEPES as buffering agent, replacing Tris) under continuous light (~200 µE m-2 s-1) and constant supply of air with 5% CO2 (gas mixture was provided by a B-DCU Biostat fermenter, Sartorius, Goettingen, Germany, and controlled via a GC system, Agilent Technologies 3000 micro-GC, Waldbronn, Germany). After the cell culture had reached an optical density of 0.5 at 750 nm (approximately 3x106 cells · mL-1), 900 mL were sampled for e.g. transcript profiling, and the concentration of CO2 in the remaining culture was reduced to 0.04% in air. Samples of 20 mL of cell culture were then collected after 60 min and 120 min for transcript profiling, and 1 L of culture was sampled after 180 min for transcript profiling and FAIRE. Cells harvested for transcript analysis were collected by centrifugation (in 2-mL aliquots) at 3,000 g for 2 min and the cell pellets were then immediately frozen in liquid nitrogen. The samples were kept at -80°C until further use. Cells (900 mL) from the last time point (180 min) were directly subjected to formaldehyde crosslinking for FAIRE (see description below).
ENA-FIRST-PUBLIC
2013-11-05
ENA-LAST-UPDATE
2012-10-02
External Id
SAMEA2189640
INSDC center alias
UNIANDES_INGQUI
INSDC center name
UNIANDES_INGQUI
INSDC first public
2013-11-05T17:01:23Z
INSDC last update
2012-10-02T14:45:14Z
INSDC status
public
Submitter Id
CHLRECCM1
broker name
La Universidad de los Andes, Colombia
mating_type
mt+
sample name
CHLRECCM1
strain
cc503 cw92 mt+