Mus musculus
Protocols: Pancreas tissue was collected from the animals sacrificed at the end of the experiment. Isolation of the pancreatic stellate cells (PSCs) was performed by gradient centrifugation using Nycodenz solution. The cells layer was collected and purified by negative selection using CD45 microbeads and MACS LD column. To induce pancreatitis, animals were injected with three episodes of caerulein (50μg/kg six time each episode) per week over two weeks. To activate the cre recombinase, animals received daily tamoxifen treatment for 5 days during the first week. All animals were sacrificed 3 weeks after the first injection episode. The total RNA from cells was extracted with the RNeasy Mini kit (Qiagen) according to the manufacturer's protocol. Library preparation: Random primed cDNA library- Purification of poly-A containing mRNA molecules- mRNA fragmentation- Random primed cDNA synthesis- Adapter ligation and adapter specific PCRamplification
ENA-FIRST-PUBLIC
2019-03-15
ENA-LAST-UPDATE
2022-06-30
External Id
SAMEA5044497
INSDC center alias
Institute of Physiological Chemistry, University of Ulm
INSDC center name
Institute of Physiological Chemistry, University of Ulm
INSDC first public
2019-03-15T17:02:25Z
INSDC last update
2022-06-30T00:13:18Z
INSDC status
public
Submitter Id
E-MTAB-7337:129
age
13
broker name
ArrayExpress
cell_type
pancreatic stellate cell
common name
house mouse
disease
chronic pancreatitis
genotype
Col1a2Cre.ERT tg/+; NEMOwt/wt
isolate
not applicable
organism part
pancreas
phenotype
wild type phenotype
sample name
E-MTAB-7337:129
sex
male
strain
C57BL/6