Mus musculus
Protocols: Lin- Sca1+ CD81+ and Lin- Sca1+ and CD81- cells were sorted from scWAT, vWAT and BAT of wildtype C57BL6 mice using FACS. Total RNA was isolated from sorted CD81+ and CD81- cells from wild type C57BL/6 mice at 10-weeks-old using RNeasy Micro Kit (QIAGEN). cDNA synthesis and amplification was performed from 50 ng of total RNA using SMARTer® Universal Low Input RNA Kit (Clontech) according to manufacturer's instruction.
ENA-FIRST-PUBLIC
2019-12-25T04:02:59Z
ENA-LAST-UPDATE
2019-10-15T12:03:08Z
External Id
SAMEA6086164
INSDC center name
UCSF
INSDC first public
2019-12-25T04:02:59Z
INSDC last update
2019-10-15T12:03:08Z
INSDC status
public
Submitter Id
E-MTAB-8435:Ing-CD81-Neg-R2
age
10
broker name
ArrayExpress
cell type
adipose precursor cell
common name
house mouse
developmental stage
adult
genotype
wild type genotype
organism part
subcutaneous adipose tissue
phenotype
CD81 negative
sample name
E-MTAB-8435:Ing-CD81-Neg-R2
scientific_name
Mus musculus
sex
male
strain
C57BL/6J