marine metagenome
ENA-FIRST-PUBLIC
2019-09-14T04:03:37Z
ENA-LAST-UPDATE
2019-09-13T16:48:02Z
External Id
SAMEA5954115
INSDC center name
PANGAEA Data Publisher for Earth and Environmental Science
INSDC first public
2019-09-14T04:03:37Z
INSDC last update
2019-09-13T16:48:02Z
INSDC status
public
Submitter Id
eDNAB0003211
access and benefit sharing, agreements and permits
not-available
access and benefit sharing, relevant Nagoya protocol
not-available
access and benefit sharing, sampled in Exclusive Economic Zone (EEZ)
not-available
acknowledgements, broker name
Stephane PESANT
acknowledgements, collected by
Ifremer, Florence PRADILLON (Florence.Pradillon@ifremer.fr)
acknowledgements, funding
eDNABYSS has received funding from Ifremer through project Pourquoi pas les Abysses, as well as ship time and transit time from the French Fleet Commission.
acknowledgements, funding
eDNABYSS has received funding from France Genomique (ANR-10-INBS-09) and Genoscope-CEA
acknowledgements, funding
The curation of eDNABYSS provenance metadata was funded by the European Union's Horizon 2020 research and innovation programme under grant agreement No 678760 (ATLAS). This output reflects only the author's view and the European Union cannot be held responsible for any use that may be made of the information contained therein.
acknowledgements, project name
eDNABYSS
checklist_accession
ERC000011
environmental condition, oxygen
not-provided
environmental condition, pH
not-provided
environmental condition, salinity
not-provided
environmental condition, temperature
not-provided
environmental material (ENVO:00010483)
seawater (ENVO:00002149) above marine sediment
environmental material label
not-provided
environmental material, depth above sediment surface, bottom
70
environmental material, depth above sediment surface, top
90
environmental material, depth below sediment surface, bottom
not-available
environmental material, depth below sediment surface, top
not-available
environmental material, quantity, volume
7140
environmental material, quantity, weight
not-available
environmental system, biome (ENVO:00000428)
marine benthic biome (ENVO:01000024)
environmental system, feature (ENVO:00002297)
marine hydrothermal vent biome (ENVO:01000030)
environmental system, marine region
[TPO] Tropical Pacific Ocean (MRGID:18071), Papua New guinea EEZ (MRGID 8324), Lau basin, Tu'i Malila vent field
last update date
2019-09-13Z
sample comment
sampling bowl not completely closed after sampling
sample description
This sample (eDNAB0003211) was collected at station PL722 during campaign CHUBACARC, using a water-sampler-(in-situ-pump-SALSA)-deployed-with-ROV. The sampling event occurred at position latitudeN=-21.9893 and longitudeE=-176.5683, on date/time=2019-04-04T05:30, at a depth of 1880 m. The sample material was collected in the marine biome (ENVO:00000447), targeting a layer of seawater (ENVO:00002149) above marine sediment, 90-70 cm above the seabed surface. The sample material was size-fractionated (5-1000 µm), packaged in a cryotube labelled CHU_PL03_SALSA2-3_Bol5, with no addition of chemical, stored in a freezer (-80degC), and sent to Ifremer, Brest, France. This sample may be used for metabarcoding analysis.
sample name
eDNAB0003211
sample protocol, chemical treatment
no addition of chemical
sample protocol, description
not provided
sample protocol, label
SEQ-METAB_WAS_5-1000
sample protocol, size fraction lower threshold
5
sample protocol, size fraction lower threshold, device
nylon mesh with 5-micrometer-pore-size in situ, and re-concentrated on board using a 47-mm-diameter polycarbonate membrane with 3-micrometer-pore-size (Isopore ref:TSTP)
sample protocol, size fraction upper threshold
1000
sample protocol, size fraction upper threshold, device
nylon mesh with 1000-micrometer-pore-size on board
sample protocol, target analysis
metabarcoding analysis
sample protocol, temperature treatment
in a freezer (-80degC)
sample storage, container label
CHU_PL03_SALSA2-3_Bol5
sample storage, container type
cryotube
sample storage, location
Ifremer, Brest, France
sampling campaign, label
CHUBACARC
sampling campaign, plateform
RV L'Atalante (http://www.flotteoceanographique.fr/La-flotte/Navires/Navires-hauturiers/L-Atalante)
sampling campaign, report
not-available
sampling event, date-time, start
2019-04-04T05:30
sampling event, date/time, end
2019-04-04T11:06
sampling event, device label
water-sampler-(in-situ-pump-SALSA)-deployed-with-ROV
sampling event, elevation, depth below sea surface
1880
sampling event, label
CHU_2019-04-04T05:30_PL722_S2-3B5_water-sampler-(in-situ-pump-SALSA)-deployed-with-ROV
sampling event, latitude, end
-21.9893
sampling event, latitude, start
-21.9893
sampling event, longitude, end
-176.5683
sampling event, longitude, start
-176.5683
sampling station, label
CHU-PL722
scientific_name
marine metagenome