Mus musculus
Protocols: TDG KO mice contain a deletion of the coding sequence for the catalytic domain of TDG, which is replaced by a neomycin-resistant cassette. Genotyping was carried out using DNA isolated from the yolk sac as template with the following primers:TDG KO/WT forward: 5′-GGGCAGCAAGGATCTGTCTA-3′TDG KO/WT reverse: 5′-GCCAGTTCCTCTGACACTTAGC-3′Mice were maintained as heterozygotes and intercrossed to generate homozygous knockout embryos for analysis. Tissues were collected from timed matings with the day of the copulation plug as E0.5. Embryos were dissected into hindbrain and heart. Tissues were collected from timed matings with the day of the copulation plug as E0.5. Embryos were dissected into hindbrain and heart. Total RNA for each sample was extracted using RNeasy mini kit (QIAGEN) and following the manufacturer's instructions. For RNA-seq, total RNA for each sample was extracted using RNeasy mini kit (QIAGEN) and following the manufacturer's instructions. Extracted RNA was polyA-enriched. Then, RNA was used for library preparation using the TruSeq RNA Library Prep Kit v2 (Illumina) following the manufacturer's instructions.
ENA first public
2018-10-22
ENA last update
2017-12-07
External Id
SAMEA104420762
INSDC center alias
Senior research associate
INSDC center name
Senior research associate
INSDC first public
2018-10-22T17:03:09Z
INSDC last update
2017-12-07T13:57:47Z
INSDC status
public
Submitter Id
E-MTAB-6271:rnaseq_brain_KO_3
age
embryonic day 11.5
broker name
ArrayExpress
common name
house mouse
genotype
Tdg knockout
organism part
hindbrain
sample name
E-MTAB-6271:rnaseq_brain_KO_3
sex
mixed