Homo sapiens
Protocols: Human osteosarcoma (U2OS) cells were obtained from CRUK and grown at 37C according to standard cell culture protocols. U2OS cells were grown at 37C and either left untreated or incubated at 43C for 4 hours to induce heat shock. Qiagen RNeasy mini kit according to manufacturer's instructions including DNAseI digestion step Isolated total RNA was incubated with DNaseI to digest residual genomic DNA. Poly-A mRNA was purified from the total RNA sample using oligo(dT) coated magnetic beads and eluted with Tris-HCl and heating. Isolated mRNA was fragmented and used for reverse transcription of cDNA (first and second strand cDNA synthesis). Purified cDNA was subjected to end-repair and A-tailing followed by adaptor ligation. Adaptor-ligated cDNA fragments were then PCR amplified and quality and quantity of the cDNA library was validated on an Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-time PCR system, respectively.
ENA-FIRST-PUBLIC
2015-07-16T15:54:27Z
ENA-LAST-UPDATE
2018-03-08T17:18:51Z
External Id
SAMEA2422481
INSDC center name
Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, United Kingdom
INSDC first public
2015-07-16T15:54:27Z
INSDC last update
2018-03-08T17:18:51Z
INSDC status
public
Submitter Id
E-MTAB-2349:growing_U2OS_cells_6
broker name
ArrayExpress
cell line
U2OS
cell type
human osteosarcoma cell
common name
human
individual
43-4
sample name
E-MTAB-2349:growing_U2OS_cells_6
scientific_name
Homo sapiens
sex
female
specimen with known storage state
fresh specimen