Mus musculus
Protocols: Self-maintaining macrophages were sorted on a BD AriaIII (BD Biosciences) in DMEM containing 30% FBS before being pelleted and resuspended in PBS with 0.04% BSA. FACS isolated cells were immediately counted by a Luna-FL Dual Fluorescence Cell Counter (Logos Biosystems). Single cell suspensions were converted to barcoded Drop-seq libraries by using the Chromium Single Cell 3â€™ Library, Gel Bead & Multiplex Kit and Chip Kit (10X Genomics; Pleasanton, California, USA). Samples were processed using kits pertaining to the V2 barcoding chemistry of 10X Genomics.
ENA-FIRST-PUBLIC
2018-07-27T17:01:53Z
ENA-LAST-UPDATE
2018-07-11T14:46:46Z
External Id
SAMEA4786373
INSDC center name
Translational Research Center for Gastrointestinal Disorders (TARGID) Department of Chronic Diseases, Metabolism and Ageing KU Leuven Belgium
INSDC first public
2018-07-27T17:01:53Z
INSDC last update
2018-07-11T14:46:46Z
INSDC status
public
Submitter Id
E-MTAB-6976:Sample 1
age
35
broker name
ArrayExpress
cell type
macrophage
common name
house mouse
genotype
Cx3cr1CreERT2.Rosa26-LSL-YFP
organism part
lamina propria of large intestine
phenotype
YFP+ CD64+
sample name
E-MTAB-6976:Sample 1
scientific_name
Mus musculus
sex
female
stimulus
tamoxifen
strain
C57BL/6