Escherichia coli str. K-12 substr. MG1655
Protocols: An overnight culture of E. coli TOP10-pBIC1a was diluted 100-fold in fresh BHI broth and allowed to grow to an OD600 of 0.5. The culture was then split and imipenem (Imipenem-Cilastatin, Mylan, USA) was added to one of the cultures at a final concentration of 5 µg/ml. Ten minutes later, 2 ml of each culture were taken and mixed with RNA protect (Qiagen, Courtaboeuf, France) according to the manufacturer's recommendations. Cells were immediately pelleted by a 10 min centrifugation at 5000 g. RNA was extracted using GeneJet RNA extraction kit (ThermoScientific). DNAse treatment was performed with the dsDNAse kit (ThermoScientific) cDNA libraries were prepared using the NEBNext® Ultra Directional RNA Library Prep Kit for Illumina protocol (New England Biolabs, MA, USA). The quality of the cDNA was validated using the Agilent 2100 Bioanalyzer DNA1000 kit (Agilent Technologies) and quantity was determined with the Qubit dsDNA BR Assay (ThermoFisher Scientific).
ENA-FIRST-PUBLIC
2018-09-15T17:01:49Z
ENA-LAST-UPDATE
2018-09-05T15:48:59Z
External Id
SAMEA4886521
INSDC center name
Bicetre Hospital, EA7361
INSDC first public
2018-09-15T17:01:49Z
INSDC last update
2018-09-05T15:48:59Z
INSDC status
public
Submitter Id
E-MTAB-7190:Sample 4
broker name
ArrayExpress
genotype
pBIC1a
growth condition
cell suspension culture
sample name
E-MTAB-7190:Sample 4
scientific_name
Escherichia coli K-12
strain
K-12
substrain
MG1655