Mus musculus
Protocols: The preparation of the material was as described in Kutejova et al., 2008. First branchial arches are dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. ChIP assays were performed as described in Kutejova et al., 2008, with the following modifications for ChIP-seq. The crosslinked material was sonicated to 300 bp fragments (Vibracell sonicator, Sonics: 8 times for 10 seconds at 50% output). Immunoprecipitation was performed starting from 12 pairs of first branchial arches from E11.5 embryos and incubating overnight at 4oC with 3 ug of pan-Meis antibodies or control IgG (Kutejova et al., 2008), followed by protein A beads (Invitrogen). Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982.
ENA-FIRST-PUBLIC
2015-02-02T17:02:16Z
ENA-LAST-UPDATE
2018-03-08T16:23:23Z
External Id
SAMEA1970328
INSDC center name
University of Manchester
INSDC first public
2015-02-02T17:02:16Z
INSDC last update
2018-03-08T16:23:23Z
INSDC status
public
Submitter Id
E-MTAB-1631:Meis IBA input exp2
broker name
ArrayExpress
common name
house mouse
developmental stage
embryo
gestational age
11.5 day
organism part
first branchial arch
sample name
E-MTAB-1631:Meis IBA input exp2
scientific_name
Mus musculus
sex
mixed sex