Prochilodus magdalenae
De novo transcriptome analysis of Prochilodus magdalenae (Steindachner, 1879) using RNA-seq.
strain
P. magdalenae
cultivar
Institute of Fish Culture Research-CINPIC
age
2 years
dev_stage
adult
sex
female
tissue
Gonad
biomaterial_provider
Institute of Fish Culture Research - CINPIC
birth_date
Monteria: Cordoba
birth_location
Institute of Fish Culture Research - CINPIC
collected_by
University of Cartagena
collection_date
2016-11-10
death_date
10-Nov-2016
disease
None
disease_stage
None
geo_loc_name
Colombia: University of Cordoba. Monteria-Cordoba
health_state
Fish in a healthy state
isolation_source
This study was conducted at the Fish Research Center of the University of Cordoba (CINPIC; Monteria, Colombia), two-year-old female bocachico were kept in earthen ponds (density: 0.5 kg / m2).
lat_lon
8.79 N 75.86 W
sample_type
tissue sample
specimen_voucher
Electronic images
store_cond
The fish was anesthetized by loss of swimming line by gradually decreasing the water temperature with ice cubes, then euthanized and dissected by an incision in the ventral midline of the fish, from the interopercular space to the anus obtaining the gonadal tissue. This gonad sample was immediately subjected to RNA extraction, with the TRIzol reagent -Invitrogen- according to the manufacturers specifications. The sample was transported in dry ice at -80grados C to the National Center for Genomic Sequencing-CNSG of the University of Antioquia for the analysis of the gonadal transcriptome of P. magdalenae fish through the second generation Sequencing Service. Sequencing service requested by the University of Cartagena, Environmental and Computational Chemistry group.
BioSampleModel
Model organism or animal