Arabidopsis thaliana
Protocols: Samples for RNA isolation and RNA-seq analysis were harvested on the day 20 and the day 25 after sowing at 11:00 AM. Plants were grown under controlled conditions in the growth chamber at 22 °C with 65% relative humidity, a light intensity of 110 µmol m-2 s-1, and the photoperiod of 16 hours light and 8 hours dark. No treatment was applied For RNA sequencing, RNA was isolated using TriFastTM (Peqlab, Erlangen, Germany) following the manufactures instructions after homogenization of the plant material. Plant material was lysed in 1 ml TriFastTM reagent per 100 mg plant tissue. After vortex and incubation for 5 minutes at RT. 200 µl chloroform was added and the solution was shaken for 15 seconds and incubated for 5 minutes at RT. After centrifugation at 4700 rpm, the aqueous phase containing RNA was separated and transferred to a new tube. Precipitation of RNA was done by adding 500 µl isopropanol. After centrifugation at 4700 rpm at 4 ˚C, the RNA pellet was washed with 70% ethanol. The isolated RNA was stored in at -80 ˚C. Strand-specific cDNA library
ENA-FIRST-PUBLIC
2020-05-14T04:05:26Z
ENA-LAST-UPDATE
2020-04-09T20:45:08Z
External Id
SAMEA6656313
INSDC center name
UNIVERSITY OF KIEL
INSDC first public
2020-05-14T04:05:26Z
INSDC last update
2020-04-09T20:45:08Z
INSDC status
public
Submitter Id
E-MTAB-8912:Sample 9
age
20
broker name
ArrayExpress
common name
thale cress
developmental stage
inflorescence development stage
ecotype
ws-4
genotype
poco1 mutant FLAG_465F03
organism part
rosette leaf
sample name
E-MTAB-8912:Sample 9
scientific_name
Arabidopsis thaliana