mouse gut metagenome
C57BL/6N male mice (8 weeks old), from Charles River (Calco, Italy and Cedex-L'Arbresle, France) were housed in both ventilated and metabolic cages, in quiet rooms with controlled temperature, humidity, and 12 h light/dark cycle, and fed for 6 weeks either with irradiated diet containing 50% saturated to unsaturated fatty acids (SFA diet) (D12450H, Research Diets Inc., Brogaarden, Gentofte, Denmark; that is equivalent in composition to the low fat diet normally used as control diet in the high fat diet-induced obesity studies) or with an identical, isocaloric, isolipidic, and isonitrogenous diet, in which protein (casein) was almost completely (93.5 %) replaced by defined free essential amino acids (SFA-EAA diet) (D14032501, Research Diets Inc.) or in which casein was completely replaced by its free amino-acids content (SFA-CAA). Fresh fecal samples was collected after 6 weeks and stored at -80°C until processing.
ENA-FIRST-PUBLIC
2019-03-20T17:02:20Z
ENA-LAST-UPDATE
2018-03-22T14:26:59Z
External Id
SAMEA104712364
INSDC center name
FONDAZIONE EDMUND MACH
INSDC first public
2019-03-20T17:02:20Z
INSDC last update
2018-03-22T14:26:59Z
INSDC status
public
Submitter Id
SFA-CAA10
dev stage
8 weeks old
sample name
SFA-CAA10
scientific_name
mouse gut metagenome
sex
male
tissue type
feces