Mus musculus
Protocols: Mice culled by anaesthetizing with isofluorane followed by cervical dislocation. Brain removed by cutting coronally at approximately bregma -0.1 and -3.0. The slice was orientated with the caudal side face up and a hypothalamic punch of 3 mm in diameter was taken from the ventral aspect. Punches were transferred to a microfuge tube immediately and frozen on dry ice. Tissue was stored at -70 degrees Celsius until processed. Sixteen wild type C57BL/6J mice, 3-4 weeks old (Harlan), singly housed and acclimatized (12:12 light:dark) on low fat diet (10% fat) for two weeks. Eight mice then switched to high fat diet (60% fat) and eight mice remain on low fat diet (10% fat). Diets continued for 20 weeks. RNA was extracted from the whole hypothalamic block using Machery-Nagel RNA isolation kit as per manufacturer's instructions. Libraries were prepared for sequencing using the Illumina TruSeq Stranded mRNA kit.
ENA-FIRST-PUBLIC
2018-10-23T17:03:42Z
ENA-LAST-UPDATE
2018-06-21T10:02:11Z
External Id
SAMEA4752159
INSDC center name
Centre for Genome Enabled Biology and Medicine University of Aberdeen
INSDC first public
2018-10-23T17:03:42Z
INSDC last update
2018-06-21T10:02:11Z
INSDC status
public
Submitter Id
E-MTAB-6929:14
age
25 to 26
broker name
ArrayExpress
common name
house mouse
diet
low fat diet
disease
normal
genotype
wild type genotype
organism part
hypothalamus
sample name
E-MTAB-6929:14
scientific_name
Mus musculus
sex
male
strain
C57BL/6J