Mus musculus
Protocols: Subventricular zone (SVZ) tissue and meninges tissue was isolated from postnatal day 0 (P0) mice and processed for RNA preparation (SVZ-bulk and meninges-bulk, respectively). Prominin-positive cells were isolated from P0 SVZ tissue, grown to neurospheres in suspension culture (SVZ-prominin neurospheres) and used for RNA extraction. Similarly, PDGFRb-positive cells from meninges tissue were grown in suspension cultures to PDGFRb-neurospheres, which were then used for RNA extraction. RNA was extracted using TRIzol (Life Technologies). Starting from total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries using the KAPA stranded mRNA-seq kit (Sopachem, Eke, Belgium).
ENA first public
2016-11-23
ENA last update
2016-07-21
External Id
SAMEA4351231
INSDC center alias
Vesalius Research Center, VIB Department of Oncology, KU Leuven
INSDC center name
Vesalius Research Center, VIB Department of Oncology, KU Leuven
INSDC first public
2016-11-23T17:01:54Z
INSDC last update
2016-07-21T15:01:42Z
INSDC status
public
Submitter Id
E-MTAB-4951:rCAR_DEW_015
broker name
ArrayExpress
common name
house mouse
fraction
neuronal cell types (heterogeneous)
organism part
subventricular zone
sample name
E-MTAB-4951:rCAR_DEW_015