Mesorhabditis belari
High molecular weight DNA was used to construct four 1D libraries for Oxford Nanopore Technologies (ONT) sequencing following the SQK-LSK108 protocol and recommendations. No preliminary fragmentation step was done. To enhance the proportion of longer fragments, fragments below 1kb were removed by adding an AMPure beads purification step prior the construction of the three last libraries. Finally, four runs of 48h were performed on a MinION using one MIN-FLO106 (R9) flowcell and three MIN-FLO107 (R9.5) flowcells. Raw data were acquired with the MinION software, MinKNOW (1.7.14 version and latter). The data processing pipeline Albacore 2.2.4. was run to perform the basecalling.
ENA-FIRST-PUBLIC
2019-03-14T17:02:45Z
ENA-LAST-UPDATE
2018-12-05T15:06:00Z
External Id
SAMEA5149890
INSDC center name
LBMC UMR5239
INSDC first public
2019-03-14T17:02:45Z
INSDC last update
2018-12-05T15:06:00Z
INSDC status
public
Submitter Id
DNASeq_M_belari_JU2817_Minion
collected by
Marie-Anne Felix
collection date
2014-10-20
geographic location (country and/or sea)
France
geographic location (region and locality)
Orsay
identified by
Marie-Anne Felix
isolation source
rotten apple
sample name
DNASeq_M_belari_JU2817_Minion
scientific_name
Mesorhabditis belari
sex
male and female
strain
JU2817