Homo sapiens
Protocols: 2000 cells were sorted as 7-AAD-CD3+CD4+CD127-CD25high Treg cells and 7-AAD-CD3+CD4+CD127varCD25var effector T cells from human PBMCs blood collection straight ex vivo flow sorting none applied modified SMART-Seq2: Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems). For DNA clean-up Agencourt AMPure XP beads were used (Beckman Coulter) as above. Nextera XT DNA sample preparation and index kits (Illumina) were used for preparation of libraries
ENA-FIRST-PUBLIC
2020-03-04T04:42:37Z
ENA-LAST-UPDATE
2020-02-26T11:12:29Z
External Id
SAMEA6591067
INSDC center name
UZH
INSDC first public
2020-03-04T04:42:37Z
INSDC last update
2020-02-26T11:12:29Z
INSDC status
public
Submitter Id
E-MTAB-8819:Treg cells 2
age
25 to 35
broker name
ArrayExpress
cell type
regulatory T cell
common name
human
developmental stage
adult
disease
normal
ethnic group
Caucasian
immunophenotype
CD3+CD8-CD4+CD25+CD127-
organism part
blood
sample name
E-MTAB-8819:Treg cells 2
scientific_name
Homo sapiens
sex
male