Sus scrofa
Protocols: The uterus was flushed with 10 ml and another 50 ml of phosphate-buffered saline (autoclaved, ph 7.4). Then, endometrial samples were collected, shock frozen in liquid nitrogen and stored at - 80 °C. We analyzed the effects of gestational estradiol-17β (E2) exposure on the endometrium. Two distinct low doses corresponding to the acceptable daily intake (ADI) and close to the no-observed effect level (NOEL) and a high dose (0.05, 10 and 1000 µg E2/kg body weight daily, respectively) were orally applied to sows from insemination until sampling at day 10 of pregnancy and compared to carrier-treated controls. RNA was harvested by means of TRIzol reagent (n = 4 per group, except for the ADI group, where a repeated tissue extraction was done because of only n=3 available animals). The library preparation starting from 125 ng total RNA of each endometrial sample was performed with the Encore Complete RNA-Seq Multiplex System IB (NuGEN, AC Leek, The Netherlands) following the manufacturers' protocol.
ENA first public
2018-10-16
ENA last update
2017-11-29
External Id
SAMEA104409002
INSDC center alias
ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland
INSDC center name
ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland
INSDC first public
2018-10-16T17:02:44Z
INSDC last update
2017-11-29T14:54:36Z
INSDC status
public
Submitter Id
E-MTAB-6242:Sample 6
breed
German Landrace
broker name
ArrayExpress
common name
pig
developmental stage
adult
disease
normal
genotype
wild type genotype
organism part
endometrium
sample name
E-MTAB-6242:Sample 6
sampling time point
day 10 of pregnancy
sex
female