soil metagenome
Mineral horizon from a late successional upland white spruce forest collected in 2005.
isolation_source
mineral horizon soil site UP3A
collection_date
2005-09-07
geo_loc_name
USA: Alaska
lat_lon
64.76805555 N 148.27666666 W
samp_collect_device
2cm diameter soil corer
samp_mat_process
Soil cores were collected, pooled and kept on liquid N until lyophilized. DNA was extracted using the MoBio PowerMax kit then normalized to 4ng/uL. Each soil sample was amplified using a barcoded primer with 7 replicates each. The primers included Illumina adaptors and golay barcodes. The base PCR primers were 5.8S_LT1 (AACTTTYRRCAAYGGATCWCT) and ITS4_mod_long (AGCCTCCGCTTATTGATATGCTTAART). Samples were pooled and sequenced in one 250bp PE MiSeq run.
BioSampleModel
Metagenome or environmental