Cotesia glomerata
Female wasps were conditioned to form a memory on the host Pieris brassicae after a single or three spaced trials, and Pieris rapae after three spaced trials. At 3 time points after conditioning the RNA of the wasp heads was isolated, namely 15 minutes, 1 hour and 4 hours after conditioning. RNA was also isolated from wasps without conditioning. For each of these 10 groups 20 wasps were taken and 3 biological replicates were made, resulting in 30 RNA samples originating from 600 female wasps. The sequence data of these 30 samples was pooled to assemble the transcriptome de novo.
breed
not applicable
host
not applicable
collection_date
2013
geo_loc_name
Netherlands: Wageningen
tissue
head
age
few days
biomaterial_provider
Laboratory of Entomology, WUR, Wageningen, The Netherlands
collected_by
Joke van Vugt
dev_stage
adult
sex
female
BioSampleModel
Invertebrate