Corynebacterium glutamicum
Protocols: Strain ATCC 13032, harvesting cells after cultivation and/or sorting using a FACS device. First the cells were grown in BHI complex media for 5 to 6 hours. After this, the cells that were starved to iron by cultivating them under low iron conditions (1 µM) over night. In the next day the cells were used to inoculate a new culture under normal iron conditions (36 µM). In the iron shift experiments a plasmid based reporter was used. This reporter system consists of the venus gene cloned under the control of a promoter of the SOS-response gene divS. For large volumes: RNeasy Kit (Qiagen, Hilden, Germany), cell disruption was performed with glass beads For small volumes (sorted samples): NucleoZol (Machery-Nagel, Düren, Germany) was used, cell disruption was performed with lysozyme + mutanlysin large sample volumes: rRNA depletion was done using Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Epicentre/illumina, Munich, Germany) small volumes: a Protocol for Removal of rRNA from Small Amounts of Total RNA” from Clontech used All libraries were generated using: TruSeq Stranded emRNA Library Prep Kit (Illumina, Munich, Germany)
ENA-FIRST-PUBLIC
2018-09-15T17:01:47Z
ENA-LAST-UPDATE
2018-05-29T15:57:38Z
External Id
SAMEA4697098
INSDC center name
IBG-1, Forschungszentrum Julich GmbH
INSDC first public
2018-09-15T17:01:47Z
INSDC last update
2018-05-29T15:57:38Z
INSDC status
public
Submitter Id
E-MTAB-6809:Sample 44
broker name
ArrayExpress
genotype
divS-Venus
phenotype
positive
replicate
3
sample name
E-MTAB-6809:Sample 44
scientific_name
Corynebacterium glutamicum
stimulus
iron shift
strain
ATCC 13032
type-material
type strain of Corynebacterium glutamicum