Asellus aquaticus
mRNA was extracted from tissue using Trizol. cDNA was prepared using the SMARTer cDNA synthesis kit from Clontech. Amplified cDNA was purified using phenol:chloroform:isoamyl (25:24:1 v/v/v). Sequencing was performed using the Roche 454 platform.
isolate
Embryos and hatchlings from lab colony established from surface population
isolation_source
Lab colony
collection_date
2011-04
geo_loc_name
Slovenia: Planina cave
tissue
Whole embryos and hatchlings
dev_stage
Embryos and hatchlings
lat_lon
45.82 N 14.25 E
sex
pooled male and female
BioSampleModel
Invertebrate