Mus musculus
Protocols: MEFs were kept in DMEM/Hams F-10 (1:1) (PAA/GE Healthcare) supplemented with 10% FCS (Fetal Bovine Serum Gold, PAA), 1% PenStrep (Sigma). DNA was immunoprecipitated using the respective antibody in accordance to the One-Day Chip kit (Diagenode) protocol. Four individual ChIPs were pooled and precipitated DNA was purified using Qiaquick columns (Qiagen). Five nanogram of precipitated DNA was used for library preparation using the Microplex libary preparation kit.
ENA-FIRST-PUBLIC
2015-01-30T17:02:25Z
ENA-LAST-UPDATE
2018-03-08T20:33:37Z
External Id
SAMEA2785674
INSDC center name
Institute for Molecular Biology and Tumor Research
INSDC first public
2015-01-30T17:02:25Z
INSDC last update
2018-03-08T20:33:37Z
INSDC status
public
Submitter Id
E-MTAB-2970:Flag-Sp2NT_Sp2koMEFs
broker name
ArrayExpress
cell type
mouse embryonic fibroblast cell
common name
house mouse
genetic modification
deletion
genotype
delta Sp2
sample name
E-MTAB-2970:Flag-Sp2NT_Sp2koMEFs
scientific_name
Mus musculus