Oryza sativa Indica Group
Protocols: The sample material was 12 pooled fifth leaves of individual plants with three biological replicates for the control line and the epilines LR1, LR2, and LR3, which belongs to the 4th selfing of the EUE selection pedigree. Epilines LR1,LR2, and LR3, and the control line were grown in soil in a growth chamber at 26 C / 21 C (day / night) for 24 days with a 16 h light/8 h dark regime (light intensity was 300 mol m-2 sec-1, the relative humidity was kept at 71%). RNA isolation was performed using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich) according to manufacturer's instructions. The RNA-seq was done by ServiceXS B.V. (Leiden, The Netherlands). Complementary DNA sequencing libraries were prepared with the Illumina mRNA-seq Sample prep Kit according the Illumina protocol “Preparing Samples for Sequencing of mRNA” (1004898 Rev.D). DNA sequencing using the Illumina HiSeq 2000 (Solexa) was performed according manufacturer's protocols.
ENA-FIRST-PUBLIC
2020-03-05T04:06:05Z
ENA-LAST-UPDATE
2018-07-05T14:55:55Z
External Id
SAMEA4778060
INSDC center name
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
INSDC first public
2020-03-05T04:06:05Z
INSDC last update
2018-07-05T14:55:55Z
INSDC status
public
Submitter Id
E-MTAB-6983:LR3-BR1
age
24
broker name
ArrayExpress
common name
long-grained rice
cultivar
indica
developmental stage
LP.05 five leaves visible stage
genotype
LR3
individual
pool of leaves from 12 plants
organism part
leaf
sample name
E-MTAB-6983:LR3-BR1
scientific_name
Oryza sativa Indica Group