Caecidotea bicrenata
mRNA was extracted from tissue using Trizol. cDNA was prepared using the SMARTer cDNA synthesis kit from Clontech. Amplified cDNA was purified using phenol:chloroform:isoamyl (25:24:1 v/v/v). Sequencing was performed using the Roche 454 platform.
isolate
Embryos from specimens captured by hand net from cave
isolation_source
USA: Franklin County, TN
collection_date
2011-02
geo_loc_name
USA: Franklin County, TN
tissue
Whole embryos
lat_lon
35.15 N 86.10 W
sex
pooled male and female
BioSampleModel
Invertebrate