Ambystoma mexicanum
Protocols: Whole body tissue samples of Mexican axolotl salamanders (Ambystoma mexicanum) at the following developmental stages: 1. Embryo at the tail bud stage 2. Newly hatched larva 3. Larva at the limb bud stage 4. Juvenile at 8.5 centimeters 5. Adult were pooled Total RNA was isolated from tissue samples using variations of guanidinium-based protocols (MacLeod et. al., 1996; Chomczynski P and Sacchi N, 1987). All tissues were considered normal and careful efforts were in place to minimize any distortion of transcript levels caused by the collection procedure. RNA quantity, purity, and integrity of both the individual samples and the resulting pool were determined with an Agilent 2100 Bioanalyzer using the Eukaryotic Total RNA nano series II analysis kit. RNA was poly-A selected and used for Illumina random priming directional library prep using the Illumina mRNA-Seq Kit (Part #RS-100-0801).
ENA first public
2018-07-29
ENA last update
2017-10-06
External Id
SAMEA104337969
INSDC center alias
Bioinformatics Core, Memorial Sloan Kettering Cancer Center
INSDC center name
Bioinformatics Core, Memorial Sloan Kettering Cancer Center
INSDC first public
2018-07-29T17:02:18Z
INSDC last update
2017-10-06T09:34:47Z
INSDC status
public
Submitter Id
E-MTAB-6092:ambystoma mexicanum tissue pool
age
Mixed age (day 1 to 1.5 years)
broker name
ArrayExpress
common name
axolotl
developmental stage
Mixed developmental stage
disease
normal
genotype
wild type genotype
organism part
whole organism
sample name
E-MTAB-6092:ambystoma mexicanum tissue pool
sex
Mixed sex
specimen with known storage state
Fresh specimen