Caecidotea bicrenata
mRNA was extracted from tissue using the Nucleospin RNA XS kit (Macherey-Nagel). cDNA was prepared using the SMARTer cDNA synthesis kit (Clontech). Amplified cDNA was purified using phenol:chloroform:isoamyl (25:24:1 v/v/v). Sequencing was performed using the Roche 454 platform.
isolate
Adult captured by hand net from cave
isolation_source
USA: Franklin County, TN
collection_date
2011-02
geo_loc_name
USA: Franklin County, TN
tissue
Adult head
lat_lon
35.15 N 86.10 W
BioSampleModel
Invertebrate