Xenopus laevis
The establishment of cell lineages occurs via a dynamic progression of gene regulatory networks that underlie developmental commitment and differentiation. To investigate how microRNAs (miRs) function in this process, we compared miRs and miR targets at the initiation of the two major ectodermal lineages in Xenopus. We over-expressed frog embryos with either nog or cabr mRNA and cut the animal caps at stage 8 and grew the caps separately till mid-gastrula. The animal caps were lysed and total RNA was collected using Direct-zol RNA mini prep and libraries were prepared.
breed
NXR
dev_stage
mid-gastrula
sex
not applicable
tissue
animal caps
collected_by
A.K.S.
BioSampleModel
Model organism or animal