Mus musculus
Protocols: Fetal livers were dissected from embryos at day 14.5 (E14.5) of development. Fetal liver cells were filtered through a cell strainer (70uM) and directly fixed in formaldehyde. Hi-C was performed following (Lieberman-Aiden et al., Science, 2009, DOI: 10.1126/science.1181369 ) with modifications detailed in Schoenfelder et al.). For CHiC experiments promoter interactions were enriched using a biotynilated RNA bait library as described. For random ligation (RL) libraries the crosslink was reversed before the ligation step in the HiC protocol, the dilution step was omitted, and the library was enriched the same way as described for CHiC.
ENA-FIRST-PUBLIC
2015-02-01T17:01:18Z
ENA-LAST-UPDATE
2018-03-08T17:18:23Z
External Id
SAMEA2421732
INSDC center name
LRI CRUK
INSDC first public
2015-02-01T17:01:18Z
INSDC last update
2018-03-08T17:18:23Z
INSDC status
public
Submitter Id
E-MTAB-2414:CHiC_FLC_rep1_t2
broker name
ArrayExpress
common name
house mouse
developmental stage
embryonic day 14.5
organism part
fetal liver
sample name
E-MTAB-2414:CHiC_FLC_rep1_t2
scientific_name
Mus musculus
strain
C57BL/6