Hadronyche cerberea
Nucleic acid sequences and peptide sequences from venom-gland preparations from the Australian funnel-web spider Hadronyche cerberea.
ENA first public
2018-01-05
ENA last update
2017-05-11
External Id
SAMEA4067784
INSDC center alias
Institute for Molecular Biosciences (UQ)
INSDC center name
Institute for Molecular Biosciences (UQ)
INSDC first public
2018-01-05T17:02:51Z
INSDC last update
2017-05-11T06:44:19Z
INSDC status
public
Submitter Id
IMB_RL6_HCEVDL1
amount or size of sample collected
4 venom glands
broker name
Bioinformatics Resources Australia - EMBL
collection date
2012
geographic location (country and/or sea)
Australia
lat lon
33.864174 S 151.20528680 E
sample material processing
All spiders used for complementary DNA (cDNA) library construction were first anesthetized using CO2, frozen at â€“80Â°C for 10 min, and then dissected at 4Â°C. To access the venom gland, the chelicerae were first removed from the base of the structure (towards the carapace). Once separated, each chelicera was individually cut from the ventral side up to the base of the fang in order to approach the venom gland from underneath. The cheliceral muscle that surrounds the venom gland was detached to expose the isolated venom gland. Dissected venom glands were placed immediately in TRIzolÂ® reagent (Invitrogen) to be processed.
sample name
IMB_RL6_HCEVDL1