Aspergillus fumigatus
Protocols: A. fumigatus conidia were collected from solid Saboraud cultures using PBS+0.1% Tween, avoiding mycelium fragment and hyphae collection. Conidia were then inoculated in RPMI 1640 medium + 2% glucose at 2.5x105 conidia/ml concentration supplemented with either 0.1mM 089 molecule or an equal volume of DMSO. Cells were grown for 4 hours in static at 37°C. After 4 hours of growth in static at 37°C, RNA was extracted from flash frozen samples by bead beating with Trizol. For each sample, one paired-end mRNA library with approximately 150bp insert size was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina).
ENA first public
2020-03-05
ENA last update
2016-12-05
External Id
SAMEA20215168
INSDC center alias
Division of Biomedical Sciences Warwick Medical School University of Warwick
INSDC center name
Division of Biomedical Sciences Warwick Medical School University of Warwick
INSDC first public
2020-03-05T04:05:52Z
INSDC last update
2016-12-05T15:39:30Z
INSDC status
public
Submitter Id
E-MTAB-5309:A1160_Ctrl_R3
broker name
ArrayExpress
cell type
conidium
sample name
E-MTAB-5309:A1160_Ctrl_R3
strain
A1160