Homo sapiens
Protocols: Liver tissue and serum were collected at the time of liver biopsy following an overnight fast for 8-10 hours and stored at -80˚C. Diagnosis of liver disease was based on standard biochemical and serological assays and consensus histological assessment of the liver biopsy by 2 blinded, experienced pathologists. Fibrosis is measured using the modified METAVIR scoring system, where 0 is no fibrosis, 1 is portal fibrosis without septa, 2 is portal fibrosis with few septa, 3 is portal fibrosis with numerous septa without cirrhosis and 4 is cirrhosis. Alcohol code is based on the following code (from in house lab classifications): 0 = NONE; 1 = >0 - RWA (recommended weekly allowance) (140g/wk women, 210g/wk Men); 2 = > RWA for sex to <350g/wk women, <420g/wk Men; 3 = ≥350g/wk women, ≥420g/wk Men; Total RNA was isolated from human liver biopsy tissue using TRI Reagent (Sigma Aldrich), as per the manufacturer's instructions. RNA quality was assessed using the Agilent 2100 BioAnalyser and samples with an RNA integrity number ≥ 6.0 were included for RNA-sequencing. Up to 1 µg of RNA was used to synthesize mRNA libraries using the TruSeq Stranded Total RNA (Ribo-Zero GOLD) library preparation kit (Illumina), as per the manufacturer's instructions.
ENA-FIRST-PUBLIC
2018-07-28T17:03:06Z
ENA-LAST-UPDATE
2018-06-08T11:35:26Z
External Id
SAMEA4717220
INSDC center name
Institute for Molecular Bioscience (IMB) and IMB Centre for Inflammation and Disease Research, The University of Queensland
INSDC first public
2018-07-28T17:03:06Z
INSDC last update
2018-06-08T11:35:26Z
INSDC status
public
Submitter Id
E-MTAB-6863:Sample 39
age
44
alcohol consumption
0
alcohol consumption measurement
0
bmi code
1
body mass index
27.5
broker name
ArrayExpress
cigarette packs per year
30 cigarette packs per year
common name
human
diabetes
non-diabetic
disease
non-alcoholic fatty liver disease
disease staging
early
elf score
enhanced liver fibrosis score 8.73
ethnic group
Caucasian
fibrosis stage
METAVIR fibrosis score 2
organism part
liver
sample name
E-MTAB-6863:Sample 39
scientific_name
Homo sapiens
sex
male
smoker
current smoker
steatosis
steatosis