Drosophila melanogaster
Protocols: Drosophila stocks were obtained from the Bloomington Stock center: btl724 (Ghabrial and Krasnow, 2006), fas1 genomic fas+, UAS-fas-myc, fasfand1, fasfand2 (Guilgur et al., 2014), Mi{MIC}CG17716MI02461 (Venken et al., 2011), btl-Gal4 (Shiga et al., 1996), btl-mCherry-nls (gift from Emmanuel Caussinus) UAS-λBtl (Lee et al., 1996), AbdB-Gal4 (de Navas et al., 2006). The fasH124 and fasP218 mutations were isolated in a mutagenesis screen, in which a btl-Gal4 UAS-GFP UAS-Verm-mRFP chromosome (Förster et al., 2010) was mutagenized with ethyl methanesulfonate (EMS). Total RNA was isolated from 12-13h old fasP218 btl-Gal4 UAS-GFP UAS-Verm-mRFP mutant embryos and from control embryos carrying the non-mutagenized parental btl-Gal4 UAS-GFP UAS-Verm-mRFP chromosome using TRIzol Reagent (ThermoFischer) according to the supplier´s protocol. cDNA libraries were generated using the standard Illumina RNASeq protocol.
ENA first public
2016-11-19
ENA last update
2016-09-26
External Id
SAMEA4465902
INSDC center alias
UZH
INSDC center name
University of Zurich
INSDC first public
2016-11-19T17:02:30Z
INSDC last update
2016-09-26T22:26:33Z
INSDC status
public
Submitter Id
E-MTAB-5069:control 3
age
12 to 13
broker name
ArrayExpress
common name
fruit fly
developmental stage
embryo
genotype
btl-Gal4 UAS-GFP UAS-Verm-mRFP
sample name
E-MTAB-5069:control 3