Cricetulus griseus
CHO-S Cells (cGMP banked) and Media Kit (Gibco, Thermo Fisher Scientific), Lot: 556395-WCB1
sample_name
CHO-S_F8F
technical_replicate
Rep 3
biomaterial_provider
Gibco (Thermo Fisher Scientific)
collection_date
2017-05-01
cell_line
CHO-S
cell_type
Suspension cells
growth_condition
Viable cell density: 3.94 x 10^6 cells/mL, Viability: 80.4%
growth_protocol
CHO-S cells were grown in 20 ml of Gibco CD-CHO medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 400 microliter of 200 mM Ala-Gln (final concentration, 4 mM; Sigma-Aldrich, St. Louis, MI, USA) and 40 microliter of Gibco Anti-Clumping Agent (final concentration, 0.2% (v/v); Thermo Fisher Scientific) in a 125 mL flask. The flask was shaken (120 rpm) at 37 degree Celsius in a humidified 5% CO2 atmosphere.
passage_history
Passage 17, 7 days in culture