Rattus norvegicus
Protocols: For fresh specimens, hepatocytes were prepared by direct perfusion of the liver with buffered salt solution, followed by cross-linking of the diced tissue in 1% formaldehyde solution for 20 minutes, addition of 250 mM glycine and incubation for a further After homogenization of cross-linked liver tissue in a dounce tissue grinder, hepatocytes were rinsed with PBS and lysed according to published protocols {Schmidt, 2009} to solubilize DNA-protein complexes. Chromatin was fragmented to 300 bp average size 1) Immunoprecipitation. Chromatin from 0.1 g of dounced liver tissue was used for each ChIP experiment using antibodies against H3K4me3 (millipore 05-1339) and H3K27Ac (abcam ab4729) and automated 96-well protocols in an Agilent Bravo liquid handling rob
ENA-FIRST-PUBLIC
2015-01-29T17:02:59Z
ENA-LAST-UPDATE
2018-03-08T18:44:30Z
External Id
SAMEA2677882
INSDC center name
Cambridge Institute - Cancer Research UK and University of Cambridge
INSDC first public
2015-01-29T17:02:59Z
INSDC last update
2018-03-08T18:44:30Z
INSDC status
public
Submitter Id
E-MTAB-2633_rev:Rat10
broker name
ArrayExpress
common name
Norway rat
developmental stage
adult
genotype
wild type
individual
Rat10
organism part
liver
sample name
E-MTAB-2633_rev:Rat10
scientific_name
Rattus norvegicus
sex
male
specimen with known storage state
fresh specimen