Bradyrhizobium elkanii
Bradyrhizobium elkanii USDA61 was grown in peptone salts yeast extract (PSY)medium (Regensburger & Hennecke, 1983) supplemented with 50 mg/mL  polymyxin. Genistein was added to a final concentration of 10 uM. As control, cultures were treated with the corresponding amount of methanol, which was used as the solvent for genistein. After 24-hours incubation, genistein-induced cells were collected and RNAs were extracted as described by Lang et al. (Molecular Genetics and Genomics, 2008 279:203-211).
sample_name
B.elkanii_RNAseq_rhizobia_2_genistein-induced
strain
USDA 61