Mus musculus
Protocols: FACS sorting experiments for gene expression analyses were performed on a BD Aria III (BD Biosciences). Cells were immediately sorted in RLT plus lysis buffer (Qiagen) with beta-mercaptoethanol and placed on dry ice. RNA was isolated using the RNeasy Plus Micro Kit (Qiagen) according to instructions by the manufacturer. cDNA libraries were prepared SMART-Seq v4 Ultra Low Input RNA kit (Clonentech) following the manufacturer's protocol.
ENA-FIRST-PUBLIC
2018-07-26T17:01:55Z
ENA-LAST-UPDATE
2018-07-11T13:11:15Z
External Id
SAMEA4786350
INSDC center name
Translational Research Center for Gastrointestinal Disorders (TARGID) Department of Chronic Diseases, Metabolism and Ageing KU Leuven Belgium
INSDC first public
2018-07-26T17:01:55Z
INSDC last update
2018-07-11T13:11:15Z
INSDC status
public
Submitter Id
E-MTAB-6977:MuYFPminus3
age
35
broker name
ArrayExpress
cell type
macrophage
common name
house mouse
genotype
Cx3cr1CreERT2.Rosa26-LSL-YFP
individual
1
organism part
muscularis externa layer of small intestine
phenotype
YFP negative
sample name
E-MTAB-6977:MuYFPminus3
scientific_name
Mus musculus
sex
female
stimulus
tamoxifen
strain
C57BL/6