Limulus polyphemus
cDNA was prepared using the SMARTer cDNA synthesis kit (Clontech). Amplified cDNA was purified using phenol:chloroform:isoamyl (25:24:1 v/v/v). Sequencing was performed using the Roche 454 platform.
isolate
Adult captured by hand
isolation_source
USA: Indian River near Titusville, FL
collection_date
2012-04
geo_loc_name
USA: Indian River near Titusville, FL
tissue
Adult ventral eye
lat_lon
28.74 N 80.75 W
BioSampleModel
Invertebrate