Euphilomedes carcharodonta
mRNA was extracted from tissue using Trizol. cDNA was prepared using the SMARTer cDNA synthesis kit from Clontech. Amplified cDNA was purified using phenol:chloroform:isoamyl (25:24:1 v/v/v). Sequencing was performed using the Roche 454 platform.
isolate
Whole embryos from adults captured by hand net
isolation_source
USA: Half Moon Bay, CA
collection_date
2011-05
geo_loc_name
USA: Half Moon Bay, CA
tissue
Whole embryos
depth
1 m
dev_stage
Embryos
lat_lon
37.29 N 122.29 W
sex
pooled male and female
BioSampleModel
Invertebrate