Hadronyche formidabilis
Nucleic acid sequences and peptide sequences from venom-gland preparations from the Australian funnel-web spider Hadronyche formidabilis.
ENA first public
2018-07-28
ENA last update
2016-07-29
External Id
SAMEA4359021
INSDC center alias
Institute for Molecular Biosciences
INSDC center name
Institute for Molecular Biosciences
INSDC first public
2018-07-28T17:02:53Z
INSDC last update
2016-07-29T06:42:46Z
INSDC status
public
Submitter Id
IMB_RL7_HFORVD1
amount or size of sample collected
4 venom glands
broker name
Bioinformatics Resources Australia - EMBL
collection date
2012
geographic location (country and/or sea)
Australia
lat lon
20.9175738 S 142.70279559 E
sample material processing
All spiders used for complementary DNA (cDNA) library construction were first anesthetized using CO2, frozen at â80Â°C for 10 min, and then dissected at 4Â°C. To access the venom gland, the chelicerr ae were first removed from the base of the structure (towards the carapace). Once separated, each chelicera was individually cut from the ventral side up to the base of the fang in order to approach the venom gland from underneath. The cheliceral muscle that surrounds the venom gland was detached to expose the isolated venom gland. Dissected venom glands were placed immediately in TRIzolÂ® reagent (Invitrogen) to be processed.
sample name
IMB_RL7_HFORVD1