Mus musculus
Protocols: Tissues were collected from timed matings with the day of the copulation plug as E0.5. Embryos were dissected into hindbrain and heart. Genomic DNA was extracted from mouse embryonic tissues at 11.5 days using the DNeasy Blood & Tissue kit (Qiagen) Indexed DNA libraries were obtained by end repair, A-tailing and adapter ligation (NEBNext Ultra II DNA library Prep kit) following the manufacturer's guideline. Per indexed library, cytosine or fully modified 5mC, 5hmC or 5fC DNA was subsequently generated by PCR (25 cycles) using the Taq polymerase and dCtp, dmCtp, dhmCtp and dfCtp and purified by GeneJET PCR Purification Kit and eluted with ultra pure water to generate gDNA_C, gDNA_mC, gDNA_hmC and gDNA_fC input samples.
ENA first public
2018-10-22
ENA last update
2017-12-07
External Id
SAMEA104420729
INSDC center alias
Senior research associate
INSDC center name
Senior research associate
INSDC first public
2018-10-22T17:03:09Z
INSDC last update
2017-12-07T13:57:46Z
INSDC status
public
Submitter Id
E-MTAB-6271:gDNA_fC
age
embryonic day 11.5
broker name
ArrayExpress
common name
house mouse
genotype
wild type genotype
organism part
hindbrain
sample name
E-MTAB-6271:gDNA_fC
sex
mixed