Arabidopsis thaliana
Protocols: A. thaliana seeds were surfaced sterilized and sown in petri dishes with agarified (0.8% Bacto Agar, Becton Dickinson) 0.5x MS medium (Duchefa), containing 1% sucrose and 0.05% MES at pH 5.7. After 3 days of stratification at 4°C, plants were grown in a CU-22L growth chamber (Percival) under 12/12 hours (day/night) light cycle. For the heat-induction, 7 days old plants grown at 21°C were first placed for 24 hours on top of a chilling platform build-in within the growth chamber and maintained by an external mini-chiller at 4°C, but with the chamber temperature set at 6°C. This was followed by 24 hours with the chamber set at 37°C for the stressed plants, whereas control plants were moved back to 21°C. RNA was isolated using the Plant-RNAeasy kit (Invitrogen). Strand-specific libraries for RNA-seq were prepared with 2 µg of RNA using the TruSeq Stranded Total RNA Library Prep Kit (Illumina); following the provider's instructions.
ENA-FIRST-PUBLIC
2019-03-17T17:02:57Z
ENA-LAST-UPDATE
2017-04-06T09:12:44Z
External Id
SAMEA103958191
INSDC center name
Sainsbury Laboratory, University of Cambridge
INSDC first public
2019-03-17T17:02:57Z
INSDC last update
2017-04-06T09:12:44Z
INSDC status
public
Submitter Id
E-MTAB-5643:Sample 8
age
9
broker name
ArrayExpress
common name
thale cress
developmental stage
seedling
ecotype
Col-0
genotype
nrpd1-3 mutant
organism part
whole organism
sample name
E-MTAB-5643:Sample 8
scientific_name
Arabidopsis thaliana