Saccharomyces cerevisiae
Protocols: For the time course experiments, the indicated strains were grown in 450ml of SC-raffinose to mid-log phase (OD600 ~0.6) at 22℃. 50ml of cells were collected by centrifugation (raffinose 0). The remaining cells were also recovered by centrifugation, resuspended in SC media lacking any carbon source, and then transferred to media containing the indicated carbon sources. 50ml of cells were collected by centrifugation at the indicated time points. Total RNAs were isolated with hot acidic phenol/chloroform (1:1) (Sigma). Sequencing libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) after ribosomal RNA was depleted using the Ribo-Zero yeast kit (Epicenter), as recommended by manufacturers.
ENA first public
2018-10-23
ENA last update
2018-02-28
External Id
SAMEA104655543
INSDC center alias
Ewha Womans University
INSDC center name
Ewha Womans University
INSDC first public
2018-10-23T17:03:39Z
INSDC last update
2018-02-28T15:11:40Z
INSDC status
public
Submitter Id
E-MTAB-6551:PHO23WT-Glc-30
broker name
ArrayExpress
common name
baker's yeast
genotype
wild type genotype
growth condition
glucose
sample name
E-MTAB-6551:PHO23WT-Glc-30
strain
YSB787