Clostridium ljungdahlii DSM 13528
Protocols: cell pellets from 50 ml culture were collected by centrifugation at -4 °C and 9,000 rpm (Hitachi CF16RXII, Qingdao, China) for 5 min. Afterwards, pellets were frozen in liquid nitrogen and stored at-80°C. Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer's protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) ≥ 7 were subjected to the subsequent analysis. Double stranded cDNA was generated using the SuperScript Double-Stranded cDNA synthesis kit (Invitrogen) according to the manufacturer's instructions.It was performed by Oebiotech (Shanghai, China) The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. This part of experiment is carried out at Oebiotech (Shanghai, China)
ENA first public
2020-03-05
ENA-LAST-UPDATE
2022-07-28
External Id
SAMEA5424647
INSDC center alias
Shandong Provincial Key Laboratory of Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
INSDC center name
Shandong Provincial Key Laboratory of Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
INSDC first public
2020-03-05T04:04:11Z
INSDC last update
2022-07-28T09:09:48Z
INSDC status
public
Submitter Id
E-MTAB-7753:Sample 2-replicate1
broker name
ArrayExpress
growth condition
exponential phase
sample name
E-MTAB-7753:Sample 2-replicate1