Mus musculus
Protocols: SMRad51 and control primary MEFs were treated or not with dox 10 μg/mL for 6 days, prior to genomic DNA extraction. Genomic DNA extraction was performed by a standard phenol-chloroform method and followed by RNAse treatment. DNA fragments were end-repaired, extended with an 'A' base on the 3′ end, ligated with paired-end adaptors with the Bravo Platform (Agilent) and amplified. Adaptor-ligated libraries were hybridized for 40 h with biotinylated oligo RNA baits using murine SureSelect Capture (Agilent), and enriched with streptavidin-conjugated magnetic beads.
ENA-FIRST-PUBLIC
2019-12-29T04:03:34Z
ENA-LAST-UPDATE
2019-12-19T16:26:25Z
External Id
SAMEA6432039
INSDC center name
Institut Cochin
INSDC first public
2019-12-29T04:03:34Z
INSDC last update
2019-12-19T16:26:25Z
INSDC status
public
Submitter Id
E-MTAB-8625:Sample_2_DOX
broker name
ArrayExpress
cell type
mouse embryonic fibroblast cell
common name
house mouse
developmental stage
embryo
genotype
rtTA-; SMRad51-
sample name
E-MTAB-8625:Sample_2_DOX
scientific_name
Mus musculus
stimulus
doxycycline, 10 microgram per milliliter