Sample 5
Homo sapiens
Protocols: At 2 h (treated and controls), 6 h and 24 h after treatment, cells were harvested by trypsination and washed in PBS. Cell suspensions were divided into aliquots for whole-cell analysis, subcellular fractionation and RNA sequencing. A431 human cells (DSMZ #ACC-91, tested for, and found free of, Mycoplasma contamination) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, non-essential amino acids, and sodium pyruvate, all from Invitrogen. Twenty four hours after seeding, the A431 cell cultures (in triplicates) were treated with gefitinib to induce altered gene/protein expression. Controls were left untreated. Total RNA was prepared from A431 cells (aliquots from the samples run on mass spectrometry) in biological triplicates using RNeasy Mini kit (Qiagen). cDNA libraries were constructed according to a modified Illumina RNA-seq library preparation protocol described in http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0021910
ENA first public
2016-12-01
ENA last update
2016-11-30
External Id
SAMEA17821168
INSDC center alias
Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University
INSDC center name
Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University
INSDC first public
2016-12-01T17:01:25Z
INSDC last update
2016-11-30T10:06:59Z
INSDC status
public
Submitter Id
E-MTAB-5285:Sample 5
broker name
ArrayExpress
cell line
A431
cell type
epithelial cell
common name
human
disease
squamous cell carcinoma
organism part
skin
sample name
E-MTAB-5285:Sample 5