Single Amplified Genome TOSAG23-1_AIFI
Stramenopiles sp. TOSAG23-1
This cell was isolated using flow cytometry from a water sample (TARA_X000001437) collected during the Tara Oceans expedition (2009-2013) at station TARA_023 (latitudeN=42.1735 and longitudeE=17.7252), on date/time=2009-11-18T13:50, using a PUMP (High Volume Peristaltic Pump). The water sample material was collected in the marine biome (ENVO:00000447) from a deep chlorophyll maximum layer (ENVO:01000326) at a depth of 53-57 m. The water sample material was size-fractionated (5-20 micrometres) and prepared on board for later detection and identification of single-cell amplified genomes (SAGs) by sequencing (SEQ) methods. The sampled cell has been identified by pyrosequencing methods and amplified using Multiple Displacement Amplification (MDA).
Chlorophyll Sensor
0.0868
Citation
http://doi.pangaea.de/10.1594/PANGAEA.842197
ENA first public
2016-11-30
ENA last update
2016-11-25
Event Date/Time End
2009-11-18T15:46
External Id
SAMEA4557817
INSDC center alias
GSC
INSDC center name
Genoscope
INSDC first public
2016-11-30T17:01:28Z
INSDC last update
2016-11-25T10:46:24Z
INSDC status
public
Latitude End
42.1659
Longitude End
17.7285
Salinity Sensor
38.395417
Submitter Id
TARA_BCB_AIFI
depth
55
environment (biome)
marine biome (ENVO:00000447); Westerlies Biome (Longhurst 2007)
environment (feature)
deep chlorophyll maximum layer (ENVO:01000326)
environment (material)
particulate matter (ENVO:xxxxxxxx), including plankton (ENVO:xxxxxxxx)
environmental package
water
event date/time start
2009-11-18T13:50
event label
TARA_20091118T1240Z_023_EVENT_PUMP
further details
https://ws.pangaea.de/dds-fdp/rest/panquery?datasetDOI=doi.pangaea.de/10.1594/PANGAEA.842197&filterParameter=SampleID&filterValue=TARA_X000001437
latitude start
42.1735
longitude start
17.7252
marine region
(MS) Mediterranean Sea [MRGID:1905]
nitrate sensor
9999
oxygen sensor
224.266917
project name
Tara Oceans expedition (2009-2013)
protocol description
This cell was isolated using a MoFlo flow cytometer equipped with a 488 nm laser for excitation. This cell was considered as an heterotrophic protist based on the presence of SYBR Green I fluorescence and the absence of chlorophyll fluorescence. The cell was then lysed using cold KOH and its DNA was amplified using multiple displacement amplification (MDA). Flow-cytometric sorting and whole genome amplification were performed by the Bigelow Laboratory Single Cell Genomics Center.
protocol label
SCG-SEQ_W5-20
sample collection device
high volume peristaltic pump [HVP-PUMP]
sample derived from
ERS905354 (TARA_X000001437)
sample name
TARA_BCB_AIFI
sample status
This version can be used to provide data discovery services
sampling campaign
TARA_20091111Z
sampling platform
SV Tara
sampling station
TARA_023
size fraction lower threshold
5
size fraction upper threshold
20
temperature
15.705783