vmrcrcw_dox_2
Homo sapiens
Protocols: Cells were seeded 24h before treatment. Cells were either untreated or treated with Doxycycline (100 ng/ml) for 96 hours. Media was removed, cells were rinsed with ice-cold PBS and lysed in RLT buffer and stored at -80 until RNA extraction. Lysates were first run through QIAShredder columns and RNA was isolated using QIAGEN Rneasy Plus Mini Kit (containing gDNA eliminator columns). RNA-seq libraries were prepared with Illumina TruSeq Stranded total RNA Library Prep kit with RiboZero Gold according to manufacturer's instructions.
ENA-FIRST-PUBLIC
2019-06-28T04:03:10Z
ENA-LAST-UPDATE
2019-03-22T11:14:56Z
External Id
SAMEA5528471
INSDC center name
Novartis Institutes for Biomedical Research
INSDC first public
2019-06-28T04:03:10Z
INSDC last update
2019-03-22T11:14:56Z
INSDC status
public
Submitter Id
E-MTAB-7808:vmrcrcw_dox_2
broker name
ArrayExpress
cell line
VMRC-RCW
cell type
kidney cell
common name
human
developmental stage
adult
disease
clear cell adenocarcinoma
genotype
PAX8 doxycycline inducible KD
organism part
kidney
replicate
2
sample name
E-MTAB-7808:vmrcrcw_dox_2
scientific_name
Homo sapiens