s10-2-2iLiF-ips-R
Sus scrofa
A?PB?backbone?for?doxycycline?(DOX)-inducible?transgene?expression?was?constructed?by?inserting?a?tetracycline?operator.?The?cDNAs?for?human?Oct4,?Sox2,?Klf4,?c-Myc?were?subsequently?cloned?into?this?backbone?vector.?The?constucted?vectors?was?electroplated?into?106?PFFs?with?a?Nucleofector?2b?Device?(Lonza)?with?a?100-?l?kit?for?primary?fibroblasts.?At?around?day?6,?iPSC?colonies?started?to?appear.?By?day?20,?iPSCs?colonies?were?picked?for?further?expansion?and?evaluation.?
breed
crossbreed(Duroc*Rongchang)
age
20 days of Pregnancy
sex
female
tissue
fetal fibroblast-derived iPSC line
growth_protocol
2i/LIF Growth Medium
BioSampleModel
Model organism or animal