AEG34.3_October_Roots
plant metagenome
replicate 3
collection_date
2015-10
description
replicate 3
env_biome
root
env_feature
root
env_material
root
geo_loc_name
Germany: Schwaebische Alb AEG34
host
Dactylis glomerata
lat_lon
48.38114044 N 9.25682349 E
organism
plant metagenome
sample_title
AEG34.3_October_Roots
seq_methods
"Sequencing was performed on the Illumina MiSeq platform using the primers 335Fc and 769Rc. PCR conditions: 98C for 5 min; 98C for 10 s / 60C for 30 s / 72C for 30 s (20 cycles); 72C for 5 min. PCR reaction (total 25 ul); 12.5 ul NEB Next High Fidelity Master Mix, 0.5 ul of each primer, 2.5 ul of 3% BSA, 100 to 200 ng of template DNA. Sample purification: AgencourtAMPureXP extraction kit (Beckman Coulter Company), ratio of AMPure XP to PCR reaction is adapted to 0.6 to 1. Indexing PCR: 98C for 5 min; 98C for 10 s / 55C for 30 s / 72C for 30 s (8 cycles); 72C for 5 min. Products are pooled equimolar to a final concentration of 4 nM and sequenced using the MiSeq Reagent kit v3 (600 cycles) for paired end sequencing."
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.plant-associated