786o_1
Homo sapiens
Protocols: 150000 Cells were lysed in ATAC-lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) and immediately processed for tagmentation. Tagmentation was performed as described in Buenrostro et al. Curr Protoc. Mol. Biol., 2016 using the Illumina Nextera Tn5 Transposase. ATAC-seq libraries were amplified from the tagmentation reaction by PCR using the PPC (PCR Primer mix) from the Illumina Nextera Tn5 Transposase kit and adding barcodes from the Nextera Index Kit (24 indexes 96 samples). DNA was purified using AmpPure XP beads.
ENA-FIRST-PUBLIC
2019-06-28T04:03:11Z
ENA-LAST-UPDATE
2019-04-03T12:06:59Z
External Id
SAMEA5547872
INSDC center name
Novartis Institutes for Biomedical Research
INSDC first public
2019-06-28T04:03:11Z
INSDC last update
2019-04-03T12:06:59Z
INSDC status
public
Submitter Id
E-MTAB-7844:786o_1
broker name
ArrayExpress
cell line
786-0
cell type
epithelial cell
common name
human
disease
renal cell carcinoma
genotype
doxycycline inducible PAX8 knockdown
organism part
kidney
sample name
E-MTAB-7844:786o_1
scientific_name
Homo sapiens