Penaeus vannamei
To enable the identification and development of genome-wide Type I SNPs, high-quality total RNA was extracted from the uropod tissue of 30 L. vannamei individuals representing prominent industry domesticated lines, using TRIZOL Reagent (Life Technologies). RNA from each individual were pooled together in equimolar amounts before being converted to double stranded cDNA using the Mint cDNA synthesis kit (Evrogen), and normalised using the Trimmer cDNA normalisation kit (Evrogen) using manufacturers recommendations. Normalised cDNA was then sequenced using an Illumina GA-IIX at 76 bp paired-end resulting in approximately 25 gigabases of paired-end EST sequence data (10x genome coverage).
isolate
Not applicable
breed
Not applicable
host
Not applicable
isolation_source
Not applicable
collection_date
2011-03-09
geo_loc_name
Indonesia
tissue
Uropod
BioSampleModel
Invertebrate