Kuenenia stuttgartiensis anoxic membrane bioreactor enrichment culture
Candidatus Kuenenia stuttgartensis
Planktonic Kuenenia cells sustained in a anoxic membrane bioreactor. The culture was obtained by Strous et. al (2006) by inoculating a gas lift bioreactor with nitrifying activated sludge in 2002. This resulted in a enrichment of Kuenenia cells occurring as flocs. The culture was transferred into a sequencing batch reactor in 2004. The current culturing setup, in operation since 2009, utilizes a membrane bioreactor. This change in cultivation enabled obtaining planktonic cells instead of flocs which resulted in an enrichment of up to 95%.
ENA first public
2017-10-20
ENA last update
2017-10-02
External Id
SAMEA104314680
INSDC center alias
RU
INSDC center name
Radboud University Nijmegen, The Netherlands
INSDC first public
2017-10-20T17:02:48Z
INSDC last update
2017-10-02T22:07:11Z
INSDC status
public
Submitter Id
KS_RU-NIJMEGEN_1
collection date
2014-03
environment (biome)
anaerobic bioreactor
environment (feature)
anaerobic bioreactor
environment (material)
anaerobic bioreactor
geographic location (country and/or sea)
Netherlands
geographic location (latitude)
51.8238509
geographic location (longitude)
5.8685827
investigation type
metagenome
miscellaneous environmental package
miscellaneous natural or artificial environment
project name
Resolving the complete genome of Kuenenia stuttgartiensis from a membrane bioreactor enrichment using Single Molecule Real-Time sequencing
sample name
KS_RU-NIJMEGEN_1
sequencing method
Single Molecule Real-Time sequencing - PacBio RS II