TregAct_3
Mus musculus
Protocols: Regulatory T cells were induced from fresh prepared mouse CD4 T cells from spleen and lymph nodes Total RNA from rested and activated regulatory T cells (four biological replicates) were extracted using Qiagen RNeasy Mini kit according to the manufacturer's instructions and quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific Inc, #Q32866) and the Qubit RNA BR assay kit (#Q10210). RNA sequencing libraries were prepared from 500 ng of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit with Poly-A mRNA magnetic isolation (NEB #E7490) according to the manufacturer's instructions.
ENA-FIRST-PUBLIC
2020-10-23T04:04:08Z
ENA-LAST-UPDATE
2020-10-13T09:45:36Z
External Id
SAMEA7423339
INSDC center name
Department of Life Sciences Imperial College London
INSDC first public
2020-10-23T04:04:08Z
INSDC last update
2020-10-13T09:45:36Z
INSDC status
public
Submitter Id
E-MTAB-9657:TregAct_3
broker name
ArrayExpress
cell type
activated regulatory T cells
common name
house mouse
organism part
spleen and lymph node
sample name
E-MTAB-9657:TregAct_3
scientific_name
Mus musculus
strain
C57BL/6J