OT10xRNA8764427
Homo sapiens
Protocols: hPSCs were differentiated into endothelial cells capable of haemogenic potential following a small molecules-driven differentiation protocol. These cells were detached from their plates in culture using TryplE and resuspended in culture media. Cells were differentiated until the emergence of haematopoietic clusters at EHT D3, when the first samples were collected and processed for scRNAseq (CTRL1). Alternatively, they were further cultured for 48 hours applying one of the following treatments: - 0.1% DMSO (CTRL2) - 1 μM PD0332991 - 10 μM RO3306 - 4 μM Roscovitine - 2 μM GSK126 At the end of the treatment, cells were collected and processed for scRNAseq. Conversion was performed following the 10X protocol (https://www.10xgenomics.com/) Chromium 10X platform. Constructed by core facility at Wellcome Sanger Institute.
ENA-FIRST-PUBLIC
2020-05-17T17:05:10Z
ENA-LAST-UPDATE
2020-05-16T21:27:52Z
External Id
SAMEA6844878
INSDC center name
Wellcome Sanger Institute
INSDC first public
2020-05-17T17:05:10Z
INSDC last update
2020-05-16T21:27:52Z
INSDC status
public
Submitter Id
E-MTAB-82051589660429:OT10xRNA8764427
broker name
ArrayExpress
cell line
A1AT-RR
cell type
haematopoietic stem cell
common name
human
developmental stage
adult
disease
Alpha-1-antitrypsin deficiency
genotype
corrected Glu342Lys mutation
organism part
skin
progenitor cell type
induced pluripotent stem cell
sample name
E-MTAB-82051589660429:OT10xRNA8764427
scientific_name
Homo sapiens
sex
male