10406X19.txt.gz
Mus musculus
Protocols: Whole cerebellar tissue was extracted from WT and transgenic (ATXN2Q127) mice that were age matched for each experiment. Mice were anesthetized using isoflurane and sacrificed. The cerebellum was quickly dissected and placed in ice-cold extracellular solution (119 mM NaCl, 26 mM NaHCO3, 11 mM glucose, 2.5 mM KCl, 2.5 mM CaCl2, 1.3 mM MgCl2 and 1 mM NaH2PO4, pH 7.4 when gassed with 5% CO2 and 95% O2). Total RNA was isolated using miRNeasy Mini Kit (Qiagen Inc., USA) according to the manufacturer's protocol. Illumina TrueSeq Stranded Total RNA Sample Prep with Ribo-Zero rRNA Removal Kit for mouse
ENA first public
2018-01-09
ENA last update
2018-01-09
External Id
SAMEA104457896
INSDC center alias
University of Utah
INSDC center name
University of Utah
INSDC first public
2018-01-09T17:03:27Z
INSDC last update
2018-01-09T10:51:34Z
INSDC status
public
Submitter Id
E-MTAB-6293:10406X19.txt.gz
age
6
broker name
ArrayExpress
common name
house mouse
disease
Spinocerebellar ataxia type 2
genotype
ATXN2Q127
organism part
cerebellum
sample name
E-MTAB-6293:10406X19.txt.gz
sex
male
strain
B6D2