Tr/Th17+IFN/IL-12+SCFA
Homo sapiens
source_name
T cells
cell type
T cells (in vitro)
interaction
T cells in vitro
treatment
In vitro-differentiated Thr/Th17 from peripheral blood T cells were seeded in combination (2.4x10e5 cells/ml of Tregs and 1.2x10e5 cells/ml of Th17 cells) in 1ml of CM on 24-well plates. Cells were activated with 10 ng/ml of IFN-gamma and IL-12 ((R&D Systems, cat. no 212-IL/285-IF) and challenged with 1mM of total SCFA (0.7mM acetate, 0.2mM propionate, 0.1mM butyrate).