Single Amplified Genome TOSAG23-1_AIGI
Stramenopiles sp. TOSAG23-1
This cell was isolated using flow cytometry from a water sample (TARA_G000000030) collected during the Tara Oceans expedition (2009-2013) at station TARA_041 (latitudeN=14.5536, longitudeE=70.0128) on date/time=2010-03-30T10:56, using a PUMP (High Volume Peristaltic Pump) with ECOTriplet. The water sample material (saline water (ENVO:00002010), including plankton (ENVO:xxxxxxxx)) was collected at a depth of 58-62 m, targeting a deep chlorophyll maximum layer (ENVO:xxxxxxxx) in the marine biome (ENVO:00000447). The water sample material was NOT size-fractionated, preserved on board with 48% Glycine Betaine, and stored in liquid nitrogen for later detection and identification of single-cell amplified genomes (SAGs). The sampled cell has been identified by pyrosequencing methods and amplified using Multiple Displacement Amplification (MDA).
Chlorophyll Sensor
0.47554
Citation
tbd
ENA first public
2016-11-30
ENA last update
2016-11-25
Event Date/Time End
2010-03-30T12:20
External Id
SAMEA4557818
INSDC center alias
GSC
INSDC center name
Genoscope
INSDC first public
2016-11-30T17:01:28Z
INSDC last update
2016-11-25T10:46:24Z
INSDC status
public
Latitude End
14.5375
Longitude End
70.0244
Salinity Sensor
36.496162
Submitter Id
TARA_BCB_AIGI
depth
60
environment (biome)
marine biome (ENVO:00000447)
environment (feature)
deep chlorophyll maximum layer (ENVO:xxxxxxxx)
environment (material)
saline water (ENVO:00002010), including plankton (ENVO:xxxxxxxx)
environmental package
water
event date/time start
2010-03-30T10:56
event label
TARA_20100330T1056Z_041_EVENT_PUMP
further details
tbd
last update date
2014-05-01Z
latitude start
14.5536
longitude start
70.0128
marine region
(search radius=5 degrees) Marine Province:West and South Indian Shelf (MRGID:21777)
nitrate sensor
-0.224514
oxygen sensor
148.328125
project name
Tara Oceans expedition (2009-2013)
protocol description
This cell was isolated using a MoFlo flow cytometer equipped with a 488 nm laser for excitation. This cell was considered as an heterotrophic protist based on the presence of SYBR Green I fluorescence and the absence of chlorophyll fluorescence. The cell was then lysed using cold KOH and its DNA was amplified using multiple displacement amplification (MDA). Flow-cytometric sorting and whole genome amplification were performed by the Bigelow Laboratory Single Cell Genomics Center.
protocol label
PROT_NUC-SCG_W0-inf
sample collection device
PUMP (High Volume Peristaltic Pump) with ECOTriplet
sample derived from
ERS489081 (TARA_G000000030)
sample name
TARA_BCB_AIGI
sample status
This version can be used to provide data discovery services
sampling campaign
TARA_20100327Z
sampling platform
SV Tara
sampling station
TARA_041
temperature
27.117663