4_2_RNA-seq
Mus musculus
Protocols: Mice were sacrificed via cervical dislocation, and the whole brains were isolated immediately. The hypothalamic ARC tissues were obtained according to the orientation of 0.4 mm lateral, 1.60 mm posterior to bregma, 7.40 mm below the surface of the dura ARC tissues were stored in 1 ml TRIZOL (Thermo Fisher Scientific, Waltham, MA, USA) and grinded in liquid nitrogen, and were added 100 μl chloroform and fully mixed, then centrifuged with highest speed at 4 oC for 10 min. The supernatant was moved into a new tube, and added the isopropanol with same volume, and centrifuged with highest speed at 4 oC for 10 min. The precipitate was washed by 75% cold ethanol, and dissolved by appropriate DEPC water.The concentration and quality of RNA was measured by Nanodrop 2000 (Thermo Fisher Scientific) and Agilent bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). 4 μg of RNA in each group were used for library preparation by NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following manufacturer's instructions.
ENA-FIRST-PUBLIC
2020-08-20T04:04:40Z
ENA-LAST-UPDATE
2020-08-10T09:30:21Z
External Id
SAMEA7160903
INSDC center name
Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
INSDC first public
2020-08-20T04:04:40Z
INSDC last update
2020-08-10T09:30:21Z
INSDC status
public
Submitter Id
E-MTAB-9420:4_2_RNA-seq
age
4
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
wild type genotype
organism part
hypothalamic arcuate nucleus
sample name
E-MTAB-9420:4_2_RNA-seq
scientific_name
Mus musculus
sex
female
strain
C57BL/6
time
early puberty