seawater metagenome
isolation_source
seawater
collection_date
2018-04-06
geo_loc_name
Pacific Ocean: North Pacific Subtropical Gyre
lat_lon
22.8336 N 158.3768 W
samp_collect_device
Water collected with Niskin bottle; filtered under low peristaltic pressure onto a 25 mm, 0.2 m pore size polyethersulfone filter (Supor)
samp_mat_process
Sample was stored at -80C until processing. gDNA was extracted with the MasterPure Complete DNA and RNA Purification Kit (Lucigen). The 16S rRNA gene hypervariable regions V4-V5 were amplified with primers 515F-Y and 926R as recommended by Parada et al. (2016), multiplexed following the Earth Microbiome Project as in Caporaso et al. (2012). Amplicons were sequenced on an Illumina MiSeq using PE250 v2 chemistry.
samp_size
~1L
depth
175
eddy_state
Neutral
experimental_treatment
Field
BioSampleModel
Metagenome or environmental