Control E14 single cell
Mus musculus
Control E14 cells.
strain
not applicable
age
not applicable
dev_stage
not applicable
sex
male
tissue
cell culture
cell_line
E14
growth_protocol
ESCs were grown on feeder free culture condition and incubated at 37¡C and 3% O2 tension. ESCs medium (knockOut DMEM, Thermo fisher), 15% ESC qualified Fetal Bovine Serum (Thermo fisher), 2 mM L-glutamine, 1/500 home-made leukemia inhibitory factor, 0,1 mM non-essential amino acids, 0,1 mM 2-Bmercaptoethanol, 50 units/ml penicillin, 50 mg/ml streptomycin supplemented with the two inhibitors PD 0325901 (1uM) and CHIR 99021 (3uM).
sample_type
cell culture
treatment
No treatment
BioSampleModel
Model organism or animal