Sample 6
Zea mays
Protocols: Roots were collected from 14 days old seedling; Ears were collected from v8 stage, and tassels collected from v7 stage, pollen collected from r1 stage, embryo and endosperm were collected from seeds of 20 days after pollination. Tissues were immediately frozen in liquid N. For each tissue, at least 10 plants were pooled and about ~30 ears and tassels were pooled for each of 3 biological replicates. Maize B73 inbred lines were grown at CSHL Uplands Farm. Total RNA was prepared by grinding tissue in TRIzol reagent (Invitrogen 15596026) on dry ice and processed following the protocol provided by manufacturer. To remove DNA, an aliquot of total RNA was treated with RQ1 DNase (Promega M6101), followed by phenol/chloroform/iso-amyl alcohol extraction, chloroform/iso-amyl alcohol extraction using Phase Lock Gel Light tubes (5 PRIME 2302800) and ethanol precipitation. RNA precipitate was stored in -20oC freezer. Total RNA (20 μg) was used for poly(A)+ selection using oligo(dT) magnetic beads (Invitrogen 610-02), eluted in water and used for RNA-seq library construction with ScriptSeqTM kit (Epicentre SS10906) or mRNA-seq Sample Prep kit (Illumina RS-100-0801) according to the manufacturer's protocol. Libraries were amplified with 12-15 cycles of PCR, and then sequenced in two lanes on Hiseq2000 PE100 platform in woodbury Genome Center in Cold Spring Harbor Laboratory.
ENA first public
2016-06-28
ENA last update
2015-08-19
External Id
SAMEA3507402
INSDC center alias
CSHL
INSDC center name
Cold Spring Harbor Laboratory
INSDC first public
2016-06-28T17:01:37Z
INSDC last update
2015-08-19T16:19:08Z
INSDC status
public
Submitter Id
E-MTAB-3826:Sample 6
broker name
ArrayExpress
cultivar
B73
organism part
tassel
sample name
E-MTAB-3826:Sample 6