RNA-Seq of WT mouse liver at ZT16 n=2
Mus musculus
Mice were entrained to 12-hr light (L) and 12-hr dark (D) cycles for at least 2 weeks in a light-tight chamber at a constant temperature (22 plus or minus 2 degrees C). The mice were sacrificed at different time for liver isolation. The total RNA was prepared from the mouse liver using TRIzol reagent (Invitrogen) according to the manufacturer's protocol. Poly(A)-tailed RNA was isolated from the total RNA as the manufacturer's protocol, and then, purified poly(A) + RNA samples were sequenced on Illumina HiSeq 3000 sequencer.
sample_name
WT_ZT16_2
sex
male
tissue
Liver