pig_ips_11
Sus scrofa
A PB backbone for doxycycline (DOX)-inducible transgene expression was constructed by inserting a tetracycline operator. The cDNAs for human Oct4, Sox2, Klf6, c-Myc were subsequently cloned into this backbone vector. The constucted vectors was electroplatnto 106 PFFs with a Nucleofector 2b Device (Lonza) with a 100-?l kit for primary fibroblasts. At around day 6, iPSC colonies started to appear. By day 20, iPSCs colonies were picked for further expansion and evaluation.
breed
Bama Xiang
dev_stage
new born
sex
female
tissue
new born fetal fibroblast-derived iPSC line
cell_line
Dox-inducible ipsc
cell_type
iPS cells
replicate
3
BioSampleModel
Model organism or animal