Human fecal metagenome from 10 mM sulfoquinovose-amended microcosm after 73 h incubation
human gut metagenome
Microcosms were constructed using homogenized vegetarian feces from 8 individuals. Microcosm was amended with 10 mM sulfoquinovose and incubated at 37C under anoxic conditions. This metagenome was generated from DNA extracted from biomass, which was harvested 73 h post substrate addition.
host
Homo sapiens
isolation_source
10 mM sulfoquinovose-amended vegetarian human fecal microcosm after 73 h incubation
collection_date
2016-11-01
geo_loc_name
Austria: Vienna
lat_lon
48.2084 N 16.3735 E
rel_to_oxygen
anaerobe
samp_collect_device
Microcosm (Hungate tubes crimp-sealed with a rubber stopper) was subsampled using an 1 ml sterile syringe and 20G needle
samp_mat_process
Subsampled fecal biomass was pelleted by centrifugation and pellet was stored at -80C. DNA was harvested by phenol/chloroform extraction.
samp_size
1
env_broad_scale
Vegetarian human fecal microcosm
env_local_scale
Microcosm amended with 10 mM sulfoquinovose
env_medium
Fecal slurry in 10 mM bicarbonate buffer
host_age
26-55 years
host_diet
Vegetarian
host_height
160-186 cm
host_sex
pooled male and female
host_tot_mass
52-85 kg
special_diet
vegetarian
BioSampleModel
Metagenome or environmental