B0669
Mus musculus
Protocols: female offspring (3 to 6 individuals per genotype and cross direction were sacrificed at the age of 11 to 13 weeks to prepare the HYP, the VNO, and the LIV from the same individuals. All dissections were done by the same person following personal instructions and standardized protocols designed by Valery Grinevich (MPI for Medical Research Heidelberg for HYP) and Masayo Omura (MPI for Biophysics, Frankfurt for VNO). For the HYP, brains were removed and further dissection was performed on wet ice using gross anatomical borders (i.e., optic chiasm arms, optic tracts, and mammillary body) as markers to remove the HYP. For the VNO, we followed the first steps of the procedure described in Meeks and Holy (2009). Prepared tissues were immediately frozen on dry ice and kept at minus 70 degree centigrade until RNA preparation Using TRIzol Reagent with the PureLink RNA Mini Kit, invitrogen/ambion With On-Column PureLink DNase Treatment during Purification Illumina TruSeq RNA Sample Preparation Kit v2
ENA-FIRST-PUBLIC
2015-02-10T17:03:05Z
ENA-LAST-UPDATE
2018-03-08T22:45:45Z
External Id
SAMEA3232187
INSDC center name
Translational Bioinformatics Group NIHR GSTFT/KCL Comprehensive Biomedical Research Centre
INSDC first public
2015-02-10T17:03:05Z
INSDC last update
2018-03-08T22:45:45Z
INSDC status
public
Submitter Id
E-MTAB-3288:B0669
broker name
ArrayExpress
common name
house mouse
genotype
WSB/EiJ mother, PWD/PhJ father
individual
5336
organism part
liver
sample name
E-MTAB-3288:B0669
scientific_name
Mus musculus
strain
cross M.m musulus (PWD/PhJ) and M. m. domesticus (WSB/EiJ)