Sample 11
Rattus norvegicus
Protocols: 12 rats were collected from rats population and split randomly into control and exercise two equal sized groups randomly. Sample Rats in treatment group underwent a 13-week downhill treadmill running exercise programme. RNA was extracted using Trizol and RNA was purified using Qiagen RNAEasy kit. RNA samples were ribosomally depleted using Illumina's Ribo-Zero kit (human, mouse, rat. Normal input). They were then analysed using Qubit for quantity and on Bioanalyser for quality. The samples were then put through the Illumina Script Seq 2 protocol to fragment, tag and amplify the samples. They were then analysed using Qubit for quantity and on Bioanalyser for quality. Amplified libraries were multiplexed and the quality and quantity of the pool were assessed using Qubit and the Agilent 2100 Bioanalyser and subsequently by qPCR using the Illumina Library Quantification Kit from Kapa on a Roche Light Cycler LC480II according to manufacturer's instructions.
ENA-FIRST-PUBLIC
2019-06-30T04:02:33Z
ENA-LAST-UPDATE
2019-01-14T11:19:49Z
External Id
SAMEA5216310
INSDC center name
CGR, Institute of Integrative Biology, University of Liverpool, UK
INSDC first public
2019-06-30T04:02:33Z
INSDC last update
2019-01-14T11:19:49Z
INSDC status
public
Submitter Id
E-MTAB-7563:Sample 11
age
23
broker name
ArrayExpress
common name
Norway rat
developmental stage
adult
genotype
wild type genotype
individual
S23
organism part
calcaneal tendon
sample name
E-MTAB-7563:Sample 11
scientific_name
Rattus norvegicus
sex
male
stimulus
exercise
strain
Lister Hooded