UTI89 T = 15 Rep 1
Escherichia coli
Protocols: Samples were collected from cultures allowed to reach the mid-logarithmic growth phase in N-minimal media. At this time 100 uM ferric iron was added to the medium. Samples were collected prior to addition of ferric iron and at 15 and 60 minutes post addition. 4 mL of cells were collected and pelleted at these times, flash frozen, and stored at -80C until RNA extraction. Strains were grown at 37C overnight shaking in lysogeny broth. The next day, strains were subcultured in fresh N-minimal media and allowed to reach mid-logarithmic growth. Cell pellets were thawed from-80C storage and total RNA was extracted according to Qiagen RNeasy kit instructions. VANTAGE (Vanderbilt Technologies for Advanced Genomics) performed mRNA enrichment using the Illumina Ribo-Zero kit and prepared cDNA libraries with the Illumina Tru-seq stranded mRNA sample prep kit.
ENA-FIRST-PUBLIC
2020-10-23T04:06:10Z
ENA-LAST-UPDATE
2020-06-30T15:34:35Z
External Id
SAMEA7029689
INSDC center name
graduate student
INSDC first public
2020-10-23T04:06:10Z
INSDC last update
2020-06-30T15:34:35Z
INSDC status
public
Submitter Id
E-MTAB-9277:UTI89 T = 15 Rep 1
broker name
ArrayExpress
genotype
wild type genotype
organism part
whole organism
sample name
E-MTAB-9277:UTI89 T = 15 Rep 1
scientific_name
Escherichia coli
strain
UTI89