MDCKshZD6_2
Canis lupus familiaris
Protocols: MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS) Cells for lentiviral transduction were grown to 70% confluency. A final concentration of 4μg/mL polybrene (SIGMA) and 10mM HEPES were combined with lentiviral-containing supernatent pre-warmed to 37 ̊C and added to cells. After incubation at 37 ̊C/5%CO2 for 24 hours, lentiviral-containing supernatant was removed and replaced with fresh complete medium. Cells were allowed to grow for another 24 hours, before harvest. Cells were washed and resuspended in 1% FBS/PBS. A 100% population of transduced cells were obtained by sterile cell sorting for GFP tagged cells. Total RNA was extracted usign Qiagen RNAeasy kit following manufacturer's instructions. Libraries were constructed according to the Illumina TruSeq stranded mRNA protocol with poly-A enrichment.
ENA-FIRST-PUBLIC
2017-10-23T17:03:32Z
ENA-LAST-UPDATE
2017-03-28T11:43:27Z
External Id
SAMEA103942072
INSDC center name
Research Fellow Tremethick Lab Department of Genome Sciences The John Curtin School of Medical Research The Australian National University
INSDC first public
2017-10-23T17:03:32Z
INSDC last update
2017-03-28T11:43:27Z
INSDC status
public
Submitter Id
E-MTAB-5628:MDCKshZD6_2
broker name
ArrayExpress
cell line
MDCK
cell type
Epithelial cell of kidney
common name
dog
growth condition
MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS)
organsim part
kidney
sample name
E-MTAB-5628:MDCKshZD6_2
scientific_name
Canis lupus familiaris
sex
female