BAT_RT_Chow_1
Mus musculus
Protocols: All experiments were approved by the Animal Ethics Committee of the North Stockholm region. The experiments were performed on mice exposed to different physiological conditions (described below). All groups had free access to water and food and 12:12-h light-dark cycle regime. The analysis of samples from different physiological conditions was performed in parallel, as indicated in the corresponding experiments. Standard conditions. C57BL/6 male mice were bought from Charles River and were kept at 21 °C until 8 weeks of age. The samples analyzed in this study were from the wild-type mice (C57BL/6) described and metabolically characterized in 25. Before the start of the experiment, the mice were housed at 22-24 °C. At the start of the experiment, 12-week-old male mice were single-caged and transferred to 30 °C (thermoneutrality). The mice had access only to high-fat diet (45 % calories from fat, Research Diets D12451). The mice were kept at thermoneutrality for at least 25 weeks. Tissues were harvested and snap frozen. Total RNA was extracted from frozen adipose tissue (left lobe/pad) with TRI Reagent® (Sigma-Aldrich) according to the manufacturer's protocol, and RNA concentrations were measured on a Nanodrop nd-1000 spectrophotometer (Thermo-Scientific, Wilmington, DE). TapeStation was applied to validate the RNA integrity. Sequencing libraries were prepared from 100 ng of total RNA (RIN 7.5-9.4) using the TruSeq Stranded mRNA Library Prep Kit (Illumina). Quality and concentration of NGS libraries were verified using the Agilent 2200 TapeStation.
ENA-FIRST-PUBLIC
2019-08-05T04:04:01Z
ENA-LAST-UPDATE
2019-01-09T14:12:56Z
External Id
SAMEA5208222
INSDC center name
ETH ZURICH
INSDC first public
2019-08-05T04:04:01Z
INSDC last update
2019-01-09T14:12:56Z
INSDC status
public
Submitter Id
E-MTAB-7561:BAT_RT_Chow_1
age
8
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
diet
chow
genotype
wild type genotype
organism part
brown adipose tissue
sample name
E-MTAB-7561:BAT_RT_Chow_1
sampling site
interscapular fat pad
scientific_name
Mus musculus
sex
male
strain
C57BL/6
temperature
23