Sample 2
Homo sapiens
Protocols: 3 µg of RNA was treated with TURBO DNase, followed by RNeasy Minelute RNA cleanup kit and RNA quaility was confirmed using Agilent 2100 Bioanalyzer with RNA Pico 6000 kit (RIN values >8). 0.5 µg of RNA was used for Lexogen QuantSeq-REV libraries. cDNA libraries were prepared according to Lexogen QuantSeq Rev method manufacturer's protocol, using a poly(T) primer for reverse transcription. The library was sequenced using Illumina HiSeq, producing 60 nt single-end reads and 10 nt index reads. Differentiation was induced by addition of BMP4 for 48hrs followin dissociation of colonies into single cells using accutase and subsequently seeding them on Matrige-coated 6-well plates containing mTESR1 medium supplemented with 20% KnockOut Serum Replacement, Glutamax, nonessential amino acids, beta-mercaptoethanol, and 50 ng/ml BMP4. Medium was changed daily. RNA-seq
ENA-FIRST-PUBLIC
2019-03-21T17:04:00Z
ENA-LAST-UPDATE
2019-03-20T15:18:58Z
External Id
SAMEA5522857
INSDC center name
Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics
INSDC first public
2019-03-21T17:04:00Z
INSDC last update
2019-03-20T15:18:58Z
INSDC status
public
Submitter Id
E-MTAB-5116:Sample 2
broker name
ArrayExpress
cell line
H9
cell type
embryonic stem cell
common name
human
fraction
nucleoplasm fraction
growth condition
BMP4
sample name
E-MTAB-5116:Sample 2
scientific_name
Homo sapiens