Sample 5
Mus musculus
Protocols: Retinas were dissected from adult mice and dissociated using the Papain Dissociation Kit (Worthington). Retinas were dissociated into single cell suspensions and sorted into two fractions: a Muller glia-enriched fraction on the basis of Muller glia-specific tdTomato expression and a second fraction containing the remaining retinal cells that were tdTomato-negative. Both fractions were sequenced. Total RNA was isolated using TRIzol reagent (Invitrogen). RNA molecules were captured using poly-A selection. Libraries were prepared using the Clontech SMARTer low input RNA library kit (Takara).
ENA-FIRST-PUBLIC
2019-06-30T04:02:30Z
ENA-LAST-UPDATE
2018-08-17T10:23:12Z
External Id
SAMEA4836246
INSDC center name
Vanderbilt University Medical Center
INSDC first public
2019-06-30T04:02:30Z
INSDC last update
2018-08-17T10:23:12Z
INSDC status
public
Submitter Id
E-MTAB-7058:Sample 5
age
35
broker name
ArrayExpress
cell type
Muller cell
common name
house mouse
genotype
Rlbp1-CreERT2; Rosa ai14/ai14
organism part
retina
phenotype
tdTomato positive
sample name
E-MTAB-7058:Sample 5
scientific_name
Mus musculus
sex
male
strain
129/Sv