6hr post-RA KH2 ES Cell HiC
Mus musculus
6hr post-RA KH2 ES Cell HiC
strain
KH2 ES Cells, Cat. No. MES4304
age
6hr post-induction
dev_stage
Embryonic
sex
male
tissue
ES Cells
biomaterial_provider
Open Biosystems, Huntsville, AL, USA
cell_line
KH2
cell_subtype
wild-type
growth_protocol
KH2 ES cells were grown in feeder free condition using N2B27+2i media supplemented with 2000 U / ml of ESGRO (Millipore). N2B27+2i media consist of neurobasal media (21103-049, Invitrogen) , DMEM/F12 media (10565-018, Invitrogen), 0.5X N2 (17502-048, Invitrogen), 1X B27 (17504044, Invitrogen),1X b-mercaptoethanol (ES-007-E , Millipore), 1X Glutamax (10378-016) ,1X NEAA (07600, SCT), 3uM CHIR99021 (4423, Tocris), PD0325901 (72184, SCT), 0.033% BSA (15260037) Thermo Fisher scientific) (Qi-Long, 2008). Cells were seeded on gelatinized plate without feeder layer. After 48 hours media was changed to differentiation media (DMEM + 10% Serum + NEAA + 3.3uM RA) for another 6 hours.
sample_type
cell culture
treatment
HiC protocol was performed as described by the developers [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149993/]. Illumina TruSeq libraries were prepared according to manufacturer''s specifications.
biological_replicate
1
BioSampleModel
Model organism or animal