Nicking mutagenesis of TEM-1: reaction 1 (residues 201-289)
Escherichia coli
This sample corresponds to a comprehensive single-site saturation mutagenesis library of the gene TEM-1. TEM-1 was ordered as a synthetic gene cassette and cloned into an E coli expression vector upon which the mutagenesis method was carried out. This reaction targeted residues 201-289.
isolate
gene TEM-1
isolation_source
synthetic gene
collection_date
2015-11-30
geo_loc_name
USA
sample_type
Recombinant protein
BioSampleModel
Microbe, viral or environmental