wnt-2T_S3_L002_R1_001
Homo sapiens
Protocols: Illumina TRUseq method to generate highly strand-specific next-generation sequencing (NGS) libraries H9 human ESCs (H9 line) were cultured in mTeSR1 medium on Matrigel-coated plates. Colonies were passaged for maintanence by Gentle Cell Dissociation Reagent Primitive streak differentiation was induced by dissociation of colonies into single cells using accutase and subsequently seeding them on Matrige-coated 6-well plates containing mTESR1 medium supplemented with ROCKi at 100% confluency. Next day cells were washed with PBS, and the medium was changed to RPMI1640 medium supplemented with L-Glutamine, B27 without insulin, 10µM ROCK inhibitor and 10µM CHIR99021 or alternatively 200ng/ml of purified WNT3A recombinant protein. For trophoblast directed differentiation human ESCs were passaged using 0,25% Trypsin-EDTA and plated as single cells (105,000 cells/cm2) on MG coated plates in DMEM/F12 medium, supplemented with 20% KnockOut Serum Replacement, Glutamax, nonessential amino acids, beta-mercaptoethanol, and 50 ng/ml BMP4. Medium was changed daily. RNA-seq
ENA-FIRST-PUBLIC
2019-03-21T17:04:00Z
ENA-LAST-UPDATE
2019-03-20T15:31:59Z
External Id
SAMEA5522897
INSDC center name
Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics
INSDC first public
2019-03-21T17:04:00Z
INSDC last update
2019-03-20T15:31:59Z
INSDC status
public
Submitter Id
E-MTAB-5114:wnt-2T_S3_L002_R1_001
broker name
ArrayExpress
cell line
H9
cell type
embryonic stem cell
common name
human
growth condition
undifferentiated
replicate
2
sample name
E-MTAB-5114:wnt-2T_S3_L002_R1_001
scientific_name
Homo sapiens