ESN_2016-04-10Z_PL10_PBT2_water-sampler-(sterile-sampling-box)-deployed-with-ROV_water-layer-(2420.03m)-below-sea-surface_size-fractionated-(2-20µm)_eDNAB0002507
marine metagenome
sample description
This sample (eDNAB0002507) was collected at station PL10 during campaign ESSNAUT-2016, using a water-sampler-(sterile-sampling-box)-deployed-with-ROV. The sampling event occurred at position latitudeN=42.9425 and longitudeE=6.7444, on date/time=2016-04-10Z, at a depth of 2420.03 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a layer of saline water (ENVO:00002010) above marine sediment (ENVO:00002113), not-available-not-available cm below the seabed surface. The sample material was size-fractionated (2-20 µm), packaged in a petri slide (47 mm diameter) labelled ESSNAUT_PL10_PBT2_2 + ESSNAUT_PL10_PBT2_2_A + ESSNAUT_PL10_PBT2_2_B, with no addition of chemical, stored freezer (-80degC), and sent to Ifremer, Sète, France. This sample may be used for metabarcoding analysis.
sampling campaign, label
ESSNAUT-2016
sampling campaign, plateform
RV L'Atalante (http://www.flotteoceanographique.fr/La-flotte/Navires/Navires-hauturiers/L-Atalante)
sampling campaign, report
JUSTINIANO Jean-Paul (2016) ESSNAUT 2016 cruise. RV L'Atalante. http://dx.doi.org/10.17600/16000500
sampling station, label
ESN_PL10
sampling event, label
ESN_2016-04-10Z_PL10_PBT2_water-sampler-(sterile-sampling-box)-deployed-with-ROV
sampling event, date-time, start
2016-04-10Z
sampling event, date/time, end
not-provided
sampling event, latitude, start
42.9425
sampling event, latitude, end
not-provided
sampling event, longitude, start
6.7444
sampling event, longitude, end
not-provided
sampling event, elevation, depth below sea surface
2420.03
sampling event, device label
water-sampler-(sterile-sampling-box)-deployed-with-ROV
sampling event, device collection area
not-available
environmental system, biome (ENVO:00000428)
marine benthic biome (ENVO:01000024)
environmental system, marine region
[MS] Mediterranean Sea (MRGID 1905)
environmental system, feature (ENVO:00002297)
continental margin (ENVO:01000298)
environmental condition, temperature
not-provided
environmental condition, salinity
not-provided
environmental condition, oxygen
not-provided
environmental condition, pH
not-provided
environmental material (ENVO:00010483)
saline water (ENVO:00002010) above marine sediment (ENVO:00002113)
environmental material, depth below sediment surface, top
not-available
environmental material, depth below sediment surface, bottom
not-available
environmental material, quantity, volume
2.5
environmental material, quantity, weight
not-provided
environmental material label
not-provided
sample protocol, label
SEQ-METAB_WCOL_2-20
sample protocol, description
not-provided
sample protocol, target analysis
metabarcoding analysis
sample protocol, size fraction lower threshold
2
sample protocol, size fraction lower threshold, device
47-mm-diameter polycarbonate membrane with 2-micrometre-pore-size (Millipore ref:NY2004700)
sample protocol, size fraction upper threshold
20
sample protocol, size fraction upper threshold, device
material passing through a 45 mm diameter nylon membrane with 20 micrometer pore size
sample protocol, chemical treatment
no addition of chemical
sample protocol, temperature treatment
freezer (-80degC)
sample storage, container label
ESSNAUT_PL10_PBT2_2 + ESSNAUT_PL10_PBT2_2_A + ESSNAUT_PL10_PBT2_2_B
sample storage, container type
petri slide (47 mm diameter)
sample storage, location
Ifremer, Sète, France
collected_by
Ifremer, Sophie ARNAUD-HAOND (Sophie.Arnaud@ifremer.fr)
comment
This sample is a pool of samples eDNAB0000183, eDNAB0000184, and eDNAB0000185.
project name
eDNABYSS
funding acknowledgements
eDNABYSS has received funding from France Genomique (ANR-10-INBS-09) and Genoscope-CEA
funding acknowledgements
eDNABYSS has received funding from Ifremer through project Pourquoi pas les Abysses, as well as ship time and transit time from the French Fleet Commission.
funding acknowledgements
The curation of eDNABYSS provenance metadata was funded by the European Union's Horizon 2020 research and innovation programme under grant agreement No 678760 (ATLAS). This output reflects only the author's view and the European Union cannot be held responsible for any use that may be made of the information contained therein.
access and benefit sharing, sampled in Exclusive Economic Zone (EEZ)
not-provided
access and benefit sharing, relevant Nagoya protocol
not-provided
access and benefit sharing, agreements and permits
not-provided
broker name
Stephane PESANT
last update date
2019-07-01Z
checklist_accession
ERC000011
ENA-FIRST-PUBLIC
2019-08-05
ENA-LAST-UPDATE
2019-08-04