Coq9R239X_5
Mus musculus
Protocols: For the RNA-Seq we dissected the midbrain of the animals. The tissue samples were rapidly frozen in liquid nitrogen and kept them at -80ºC RNA was extracted following the TRI Reagent Solution protocol from Applied Biosystems Library preparation and sequencing was performed at the Transcriptome and Genome Analysis Laboratory, NGS Core Unit at the Universitzy Medical Center Göttingen. Quality and integrity of RNA was assessed with the Fragment Analyzer from Advanced Analytical by using the standard sensitivity RNA Analysis Kit (DNF-471). All samples selected for sequencing exhibited an RNA integrity number over 8. RNA-seq libraries were performed using 500 ng total RNA of a non stranded RNA Seq, massively-parallel mRNA sequencing approach from Illumina (TruSeq RNA Library Preparation Kit v2, Set A; 48 samples, 12 indexes, Cat. N°RS-122-2001). For accurate quantitation of cDNA libraries a fluorometric based system, the QuantiFluor™dsDNA System from Promega were used. The size of final cDNA libraries was determined by using the dsDNA 905 Reagent Kit (Fragment Analyzer from Advanced Bioanalytical) exhibiting a sizing of 300 bp in average. Libraries were pooled and sequenced on the Illumina HiSeq 4000 (SE; 1 x 50 bp; 30-35 Mio reads/sample).
ENA-FIRST-PUBLIC
2019-03-21T17:04:00Z
ENA-LAST-UPDATE
2019-03-19T14:52:39Z
External Id
SAMEA5522688
INSDC center name
Instituto de Biotecnologia, Centro de Investigacion Biomedica, Universidad de Granada, Granada, Spain
INSDC first public
2019-03-21T17:04:00Z
INSDC last update
2019-03-19T14:52:39Z
INSDC status
public
Submitter Id
E-MTAB-7794:Coq9R239X_5
age
3
broker name
ArrayExpress
common name
house mouse
developmental stage
young adult
genotype
Coq9R239X
individual
12
organism part
midbrain
sample name
E-MTAB-7794:Coq9R239X_5
scientific_name
Mus musculus
sex
female
strain
C57BL/6