Sample 13
Sus scrofa
Protocols: A total of 48 Italian Large White pigs, 24 gilts and 24 barrows, were used for this study. The pigs were selected from a progeny of 258 piglets derived from 21 sows and 3 boars registered to the herd book of the Italian National Association of Pig Breeders (ANAS). All animals used in this study were kept according to the Council Rule (EC) No 1/2005 on the protection of animals during transport and related operations and amending Directives 64/432/EEC and 93/119/EC and Regulation (EC) No 1255/97. After weaning, at the average live weight of 79.9 ± 5.8 kg, the pigs were divided into 4 groups of 12 animals each, balanced for weight, father and sex. Pigs after weaning were all fed a standard diet until the starting of the trial. During the trial period, the pigs were fed four experimental diets: a standard diet for growing-finishing pigs (D1); a diet enriched with extruded linseed (source of n-3 PUFA) (D2); a diet enriched with extruded linseed, and vitamin E and selenium as antioxidants (D3); a diet enriched with extruded linseed and plant extract from grape-skin and oregano (source of polyphenols) (D4). During the trial, one pig belonging to the experimental group fed D4 died of natural causes (sample 48). At the end of the trial, the animals were transported to a commercial abattoir. At the slaughterhouse, the pigs were electrical stunned and bled in a lying position in agreement with the Council Regulation (EC) No 1099/2009 on the protection of animals at the time of the killing. All slaughter procedures were monitored by the Veterinary team appointed by the Italian Ministry of Health. Slaughter was performed in two batches at a interval of 14 days one from the other. In order to have a similar weight for all the pigs at slaughter, in the first batch were sent the 6 heaviest pigs of each group, for a total of 24 animals. The remaining 6 pigs of each dietary group were then sent in the second batch after 14 days (23 pigs in the second batch as one died before the end of the experiment). Animals of each batch were loaded and transported together in the same truck. Groups were maintained separated inside the truck and during all the pre-slaughter procedures to avoid stress response due to unfamiliar pig mixing. At the end of the slaughter line and before the carcass cooling, a sample from the Longissimus thoracis muscle of each pig was taken and immediately frozen in liquid nitrogen. Samples were then stored at -80°C until RNA extraction. Total RNA was extracted using the standard RNA extraction method with TRIzol (Invitrogen, Carlsbad, CA, USA). Before use, RNA concentration in each sample was assayed with an ND-1000 spectrophotometer (NanoDrop Technologies) and its quality assessed with the Agilent 2100 Bioanalyzer with Agilent RNA 6000 nano kit (Agilent Technologies, Santa Clara, CA, USA). Indexed libraries were prepared from 1 µg of purified RNA from each sample with TruSeq Stranded mRNA (Illumina) Library Prep Kit according to the manufacturer's instructions. Libraries were quantified using the Agilent 2100 Bioanalyzer (Agilent Technologies) and pooled (16 samples/pool for a total of 3 pools) such that each index-tagged sample was present in equimolar amounts, with a final concentration of the pooled samples of 2nM.
ENA-FIRST-PUBLIC
2019-06-29T04:02:34Z
ENA-LAST-UPDATE
2018-09-03T16:34:14Z
External Id
SAMEA4881084
INSDC center name
Bologna University DISTAL Animal Genomics Division
INSDC first public
2019-06-29T04:02:34Z
INSDC last update
2018-09-03T16:34:14Z
INSDC status
public
Submitter Id
E-MTAB-7131:Sample 13
age
275
block
1
breed
Italian Large White
broker name
ArrayExpress
common name
pig
diet
D3 - standard diet enriched with extruded linseed and vitamin E and Selenium
organism part
longissimus thoracic muscle
sample name
E-MTAB-7131:Sample 13
scientific_name
Sus scrofa
sex
male