Human sample from Homo sapiens
Homo sapiens
We assessed whether organoids maintained the gene mutation signatures of their parental tumor. We performed Whole-exome sequencing (WES) analysis in one patient who had diagonsed as rectal adenocarcinoma. Total DNA of organoids and their parental tissues were extracted at day 7 and day 0, respectively. The results show that The tumor-derived organoids were found comprising a highly overlapped (over 97%) single nucleotide variants (SNV) profile with their parental tumor tissue. For the oncogene dataset comprising 731 genes, we found 16 genetic mutations in the P31 organoids and 15 genetic mutations in the parental tumor tissue. It suggests that the organoids recapitulated the genetic information of the parental tumor.
isolate
surgical resections
age
56 years old
biomaterial_provider
The First Affiliated Hospital of Zhengzhou University
sex
male
tissue
rectal tumor
BioSampleModel
Human