1072N0001
Homo sapiens
Protocols: Plasma was fractionated from blood and cryopreserved (-80°C). To control for the bias of miRNAs from red blood cells in differential analysis, we compiled pools so that average levels of hemolysis were comparable across groups. After exclusion of hemolysis specimens, plasma pools were formed by combining equal volumes of patients' plasma. Total RNA was extracted from 2.5 ml plasma using the miRVana PARIS technology kit (Life Technologies). NGS cDNA libraries were prepared and validated from plasma RNA by ARK Genomics (University of Edinburgh, UK), following manufacturer instructions, with specific barcodes for each cDNA library (Illumina TruSeq Small RNA sample protocol). Bands corresponding to ~22 nucleotides in the original sample were size-selected (correct insert size: 146bp) after band staining and visualization under UV-light.
ENA first public
2016-12-01
ENA last update
2016-04-18
External Id
SAMEA3932389
INSDC center alias
Brighton and Sussex Medical School (BSMS)
INSDC center name
Brighton and Sussex Medical School (BSMS)
INSDC first public
2016-12-01T17:01:24Z
INSDC last update
2016-04-18T17:17:48Z
INSDC status
public
Submitter Id
E-MTAB-4637:1072N0001
broker name
ArrayExpress
common name
human
disease
systemic inflammatory response syndrome
disease staging
severe
obsolete_sex
male/female pool
organism part
blood plasma
sample name
E-MTAB-4637:1072N0001
sample pool size
pool of 23 individuals
technical duplicate of
1072N0002