Y3_S
Homo sapiens
Protocols: Fresh colon tissues were prospectively collected from women undergoing screening colonoscopy. Colon biopsy specimens of normal mucosa were obtained from the cecum and sigmoid of six women whose colorectum did not show any neoplastic or inflammatory alterations. Biopsies for the study were placed in tubes filled with AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C prior to DNA extraction with Qiagen's AllPrep Mini Kit. For methylation analysis, sequencing libraries were prepared using 1 microgram of DNA per sample, according to the Roche SeqCapEpi CpGiant protocol (Roche, Rotkreutz, Switzerland), and bisulfite-converted prior to capture with a pre-designed probe pool (Roche-NimbleGen, Madison, WI)
ENA-FIRST-PUBLIC
2020-10-23T04:04:08Z
ENA-LAST-UPDATE
2020-10-13T11:17:35Z
External Id
SAMEA7424981
INSDC center name
Institute of Molecular Cancer Research, University of Zurich
INSDC first public
2020-10-23T04:04:08Z
INSDC last update
2020-10-13T11:17:35Z
INSDC status
public
Submitter Id
E-MTAB-9665:Y3_S
age
38
broker name
ArrayExpress
common name
human
disease
normal
individual
Y3
organism part
sigmoid colon
sample name
E-MTAB-9665:Y3_S
scientific_name
Homo sapiens
sex
female