Human fecal metatranscriptome from 10 mM sulfoquinovose-amended microcosm after 6 h incubation replicate 1
human gut metagenome
Microcosms were constructed using homogenized vegetarian feces from 8 individuals. Microcosm was amended with 10 mM sulfoquinovose and incubated at 37C under anoxic conditions. This metatranscriptome was generated from RNA extracted from biomass, which was harvested 6 h post substrate addition. Microcosm replicate 1
host
Homo sapiens
isolation_source
10 mM sulfoquinovose-amended microcosm after 6 h incubation replicate 1
collection_date
2016-11-01
geo_loc_name
Austria: Vienna
lat_lon
48.2084 N 16.3735 E
rel_to_oxygen
anaerobe
samp_collect_device
Microcosm (Hungate tubes crimp-sealed with a rubber stopper) was subsampled using an 1 ml sterile syringe and 20G needle
samp_mat_process
Subsampled fecal biomass was pelleted by centrifugation and pellet was stored at -80C. RNA was harvested by phenol/chloroform extraction followed by Dnase treatment.
samp_size
1
BioSampleModel
Metagenome or environmental