Sample 24
Homo sapiens
Protocols: PBMCs were isolated from three individuals. Cells from these biological replicates were exposed to infected RBCs, uninfected RBCs, or RPMI prior to stimulation with PAM3CSK4 (10g/mL) for 0, 4, and 12 hours. Total RNA was extracted using the Aurum Total RNA Mini Kit (BioRad). For each sample, 0.5 g of total RNA was used to prepare RNA-seq libraries using the TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat kit and the TruSeq RNA single indexes Set A (Illumina) according to the manufacturer's instructions.
ENA-FIRST-PUBLIC
2020-05-15T04:06:09Z
ENA-LAST-UPDATE
2020-05-13T21:29:32Z
External Id
SAMEA6831307
INSDC center name
Department of Medicine University of Massachusetts Medical School
INSDC first public
2020-05-15T04:06:09Z
INSDC last update
2020-05-13T21:29:32Z
INSDC status
public
Submitter Id
E-MTAB-9066:Sample 24
age
27
broker name
ArrayExpress
cell type
peripheral blood mononuclear cell
common name
human
developmental stage
adult
disease
normal
growth condition
uninfected erythrocytes
individual
3
organism part
blood
replicate
3
sample name
E-MTAB-9066:Sample 24
scientific_name
Homo sapiens
sex
female