Pacific oyster hemolymph microbiota from Texel
Magallana gigas
Pacific oyster (Crassostrea gigas) hemolymph microbiota; collected on Texel, 2012-06-14; sample taken in the lab, after the treatment and before field deployment; not antibiotic treated; oyster from Texel, not translocated
ENA first public
2016-06-29
ENA last update
2016-10-21
External Id
SAMEA3461390
INSDC center alias
ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
INSDC center name
ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
INSDC first public
2016-06-29T17:01:56Z
INSDC last update
2016-10-21T09:39:29Z
INSDC status
public
Submitter Id
Cg_34_Hem_Tx_TxC_20120614
amount or size of sample collected
0.002
collection date
2012-06-14
common name
Pacific oyster
environment (biome)
aquatic, marine, benthic, coastal
environment (feature)
organic
environment (material)
organic material, bodily fluid
geographic location (country and/or sea)
North Sea
geographic location (latitude)
53.1567452
geographic location (longitude)
4.904574865
geographic location (region and locality)
Netherlands, Texel, de Cocksdorp
host body habitat
hemolymph
host body site
adductor muscle
host common name
Pacific oyster
host life stage
adult
host subject id
Tx_34
host taxid
29159
host-associated environmental package
host-associated
investigation type
mimarks-survey
multiplex identifiers
f: AACGAACG; r: TTGGTACG
pcr conditions
initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
pcr primers
27f-338r
pcr primers
27F: TCAGAGTTTGATCMTGGCTCAG + 338r: CATGCTGCCTCCCGTAGGAGT
perturbation
not antibiotic treated
project name
PRJEB9624
sample collection device or method
syringe
sample name
Cg_34_Hem_Tx_TxC_20120614
sample storage temperature
-80
sample volume or weight for DNA extraction
0.2
sequencing method
Sequencing by synthesis, Illumina paired-end
target gene
16s rRNA
target subfragment
V1-V2
temperature
15.12717638