CD45+ cells: RFA
Mus musculus
strain
C57BL/6
age
16 weeks
sex
male
tissue
CD45+ immune cells
growth_protocol
Panc02 tumor cells were injected subcutaneously into male C57BL/6 mice at 1 x 106 cells per 200ul on bilateral flanks, respectively.
treatment
Tumor on the left flank receives RFA treatment, while the tumor on the right side did not receive any intervention.
extraction_protocol
On day 3 after RFA treatment, tumors on the right side (non-RFA side) were isolated, digested and isolated CD 45+ cells. Libraries were constructed using Single Cell 5 Library Kit.
BioSampleModel
Model organism or animal