MDCKTGFbD6_3
Canis lupus familiaris
Protocols: MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS) For TGF-beta treatment, wildtype MDCK were stimulated in culture by adding 5ng of TGF-beta to the medium. TGF-beta was added with every passage of cells, to maintain stimulus. Total RNA was extracted usign Qiagen RNAeasy kit following manufacturer's instructions. Libraries were constructed according to the Illumina TruSeq stranded mRNA protocol with poly-A enrichment.
ENA-FIRST-PUBLIC
2017-10-23T17:03:32Z
ENA-LAST-UPDATE
2017-03-28T11:43:27Z
External Id
SAMEA103942076
INSDC center name
Research Fellow Tremethick Lab Department of Genome Sciences The John Curtin School of Medical Research The Australian National University
INSDC first public
2017-10-23T17:03:32Z
INSDC last update
2017-03-28T11:43:27Z
INSDC status
public
Submitter Id
E-MTAB-5628:MDCKTGFbD6_3
broker name
ArrayExpress
cell line
MDCK
cell type
Epithelial cell of kidney
common name
dog
growth condition
MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS)
organsim part
kidney
sample name
E-MTAB-5628:MDCKTGFbD6_3
scientific_name
Canis lupus familiaris
sex
female