PoPch1
Vitis vinifera
Protocols: 2cm wood chunks were cut and snap-frozen in liquid Nitrogen untill used. Wood pieces were ground in liquid nitrogen using a a Tissue LyserII (Qiagen). Resulting powder was stored at -80AC until RNA extraction. RNA was extracted from 200 mg powder using a Qiagen RNeasy Plant Mini Kit. RNA libraries were prepared for sequencing using standard Illumina protocols. A TruSeq Stranded Total RNA with Ribo-Zero Plant kit was used.
ENA-FIRST-PUBLIC
2015-10-23T17:01:28Z
ENA-LAST-UPDATE
2018-03-09T09:21:27Z
External Id
SAMEA3598472
INSDC center name
INRA, UMR1065 Sante et Agroecologie du Vignoble (SAVE), ISVV, 33883 Villenave dOrnon, France
INSDC first public
2015-10-23T17:01:28Z
INSDC last update
2018-03-09T09:21:27Z
INSDC status
public
Submitter Id
E-MTAB-3966:PoPch1
biological replicate
1
broker name
ArrayExpress
common name
wine grape
development stage
2 month old plantlets
organism part
wood chunk
sample name
E-MTAB-3966:PoPch1
scientific_name
Vitis vinifera
treatment
Phaeomoniella chlamydospora wood infection AND Pythium oligandrum root inoculation
cultivar
Cabernet Sauvignon