118
synthetic construct
Protocols: Sequencing ready synthetic constructs with STR sequences of varying length.The constructs where sequenced as is and following several PCR amplifications. See further details in \Short Tandem Repeat stutter model inferred from direct measurement of in vitro stutter noise\ (https://doi.org/10.1101/065110) The constructs that went through PCR amplification where barcoded in that PCR in a way that over-writes the original barcodes they where synthesized with.
# repeats
18
ENA-FIRST-PUBLIC
2019-01-26T17:02:10Z
ENA-LAST-UPDATE
2019-01-16T16:45:27Z
External Id
SAMEA5226899
INSDC center name
Department of Computer Science and Applied Mathematics, Weizmann Institute of Science
INSDC first public
2019-01-26T17:02:10Z
INSDC last update
2019-01-16T16:45:27Z
INSDC status
public
Submitter Id
E-MTAB-7602:118
broker name
ArrayExpress
cloning plasmid
Modified puc57-Kan* (GenScript)
final sequence
AGTACTAAGCAATGACAATGATACGGCGACCACCGAGATCTACACCGTGAGTGACACTCTTTCCCTACACGACGCTCTTCCGATCTTCCTAATCTTACGCGGCCATAACgtcCGACTACCGTTGTCCATAGCGATAGGGGGGGGGGGGGGGGGGTCCGCAGTAGTATTGGAGCCGTATGATGGGCTCTTAAAGACTGTCCATGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGCATTGCAGTACTAA
flanking sequence (left)
CGACTACCGTTGTCCATAGCGATA
flanking sequence (right)
TCCGCAGTAGTATTGGAGCCGTAT
genotype
18
identifier barcode
gtc
name of plasmid
SyMS_18G
repeat type
G
sample name
E-MTAB-7602:118
scientific_name
synthetic construct
sequencing primer fw (illumina)
ACACTCTTTCCCTACACGACGCTCTTCCGATCT
sequencing primer rev (illumina)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
str (ms) seq
GGGGGGGGGGGGGGGGGG