Sample 2
Mus musculus
Protocols: Single-cell suspension of thymus was obtained as described (Salou et al., 2018), suspensions from 6-8 B6-MAITCast thymii were pooled prior to MR1 tetramer staining (staining performed 30 min at room temperature) followed by tetramer-based magnetic enrichment (Legoux and Moon, 2012). FACS sorting was performed on an Aria III cell sorter (BD). Total MR1 tetramer+ cells were flow-sorted into CO2-independent medium supplemented with 10% FCS. Sorted cells were centrifuged and counted, and 7,000 cells were loaded onto the Chromium 3' chip. Nucleic acid extraction was performed with the 3' v2 library kit and following the standard procedures from 10x genomics. Reverse transcription and library preparation were performed with the 3' v2 library kit and following the standard procedures from 10x genomics.
ENA-FIRST-PUBLIC
2019-07-01T17:03:54Z
ENA-LAST-UPDATE
2019-02-21T09:26:25Z
External Id
SAMEA5366092
INSDC center name
Institut Curie - INSERM U932
INSDC first public
2019-07-01T17:03:54Z
INSDC last update
2019-02-21T09:26:25Z
INSDC status
public
Submitter Id
E-MTAB-7704:Sample 2
age
6 to 10
broker name
ArrayExpress
cell type
mucosal invariant T cell
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
2
organism part
thymus
phenotype
MR1:5-OP-RUtet+
sample name
E-MTAB-7704:Sample 2
scientific_name
Mus musculus
sex
mixed
strain
B6-MAIT/CAST