Sample 25
Rattus norvegicus
Protocols: PCCl3 were maintained in Coon's modified F12 medium (EuroClone) supplemented with 5% newborn bovine serum (HyClone Laboratories) and six hormones 16. Cells were exposed for 7 days to CPF (6x10-7 M, 6x10-8 M, 6x10-9 M, Greyhound Chromatography F2057) and ETU (6x10-8 M, 6x10-9 M, 6x10-10 M, 2-Imidazolidinethione, Sigma-Aldrich I504) and their combinations. Total RNA was isolated from treated and control cells using Trizol reagent (Invitrogen) following manufacturer's instructions. Total RNA samples were treated with DNasi (NEB), purified with RNasy Mini Kit (Qiagen) and controlled with Agilent 2100 bioanalyzer (Agilent Technologies). RNAs, 3 µg/each obtained from three biological replicates, were pooled to minimize sample-to-sample variations. 1μg of each RNA pool was used for library preparation with TruSeq Stranded total RNA Sample Prep Kit (Illumina Inc.) according to the manufacturer's instructions.
ENA first public
2016-12-06
ENA last update
2016-11-24
External Id
SAMEA4557142
INSDC center alias
IRGS, Biogem
INSDC center name
IRGS, Biogem
INSDC first public
2016-12-06T17:02:11Z
INSDC last update
2016-11-24T10:53:23Z
INSDC status
public
Submitter Id
E-MTAB-5269:Sample 25
broker name
ArrayExpress
cell line
PCCl3
cell type
thyrocyte
common name
Norway rat
organism part
thyroid gland
sample name
E-MTAB-5269:Sample 25