Sequences of ATM on- and off-target genomic sites from Hek293T cells treated with canonical TALENs
Homo sapiens
Sequencing from Hubbard et al, 2015.  293 cells were transfected with a plasmid expressing homodimeric FokI fusions to the canonical WT ATM-L TALE together with a plasmid encoding the canonical ATM-R TALEâFokI fusion protein. After 48 h, genomic DNA was harvested and the ATM target site and its corresponding known off-target sites OffA11 and OffA17 were amplified from this genomic DNA.  The amplified sites werethen  high-throughput sequenced.  All genomic target sites (ATM on-target, OffA11 and OffA17) were combined together for this one type of TALEN treatment.
isolate
Hek293
age
?
biomaterial_provider
GenTarget
sex
female
tissue
Hek293
TALEN targets
ATM
genomic sites amplifed
ATM on-target, ATM OffA11, ATM OffA17
on-target sites
yes
off-target sites
yes
BioSampleModel
Human