Medicago ruthenica Control
Medicago ruthenica
Seeds of M. ruthenica were germinated, and transferred on a mix of perlite and sand. All seedlings were grown in growth chamber, and chamber temperature was between 18 C (night) and 24 C (day), the humidity ranged from 60% to 80%, and the light period was 14h/10h (day:night). The seedlings were irrigated with half-strength Hoagland solution once every two days, and after eight weeks they were selected for stress treatments. For cold stress and freezing stress, the seedlings were transferred into another chamber with temperature set at 4 C or -8 C respectively. For osmotic stress and salt stress, the seedlings were treated with 300 mM mannitol or 200mM NaCl solution respectively. For ABA treatment, the seedlings leaves were sprayed with 100 uM ABA solution. The seedlings containing control (untreated) and treated were harvested at 3 h after treatment, five whole seedlings from each group were bulked separately, and all samples were frozen in liquid nitrogen and stored at -80 C until use.
isolate
Zhilixing
age
eight weeks
geo_loc_name
China: Harbin
tissue
Whole seedling
temp
24
treatment
Control
BioSampleModel
Plant