Sample 1
Drosophila melanogaster
Protocols: 5000 eye imaginal discs were dissected at mid-3rd instar larvae for each sample followed the description in Marshall et al., 2016 doi:10.1038/nprot.2016.084 in brief, dissected eye imaginal discs were mechanically disrupted and genomic DNA was extracted with QIAamp DNA Micro Kit (qiagen# 56304), then digested with DpnI to obtain methylated DNA. methylated DNA after DpnI digestion was ligated with the designed adaptors for further PCR. Ligated DNA was first digested with DpnII and then PCR with designed primers. Designed adapter sequences and primer sequences are as follows:AdRt oligo_5' CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA3'; AdRb oligo_5'TCCTCGGCCG3'; DamID_PCR oligo_5'GGTCGCGGCCGAGGATC3'
ENA-FIRST-PUBLIC
2019-03-22T10:13:45Z
ENA-LAST-UPDATE
2018-12-05T09:01:24Z
External Id
SAMEA5147897
INSDC center name
Department of Life Sciences, National Cheng Kung University
INSDC first public
2019-03-22T10:13:45Z
INSDC last update
2018-12-05T09:01:24Z
INSDC status
public
Submitter Id
E-MTAB-7475:Sample 1
broker name
ArrayExpress
common name
fruit fly
developmental stage
third instar larva stage
genotype
C135>UAS-Dam
organism part
eye-antennal disc
sample name
E-MTAB-7475:Sample 1
scientific_name
Drosophila melanogaster