T1430
Homo sapiens
Protocols: T-cells (T-ALL and normal T lymphocytes) were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson). Total RNA was isolated using miRCURY RNA Isolation Kit Cell & Plant (Exiqon). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). Libraries were generated with NEBNext® Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, MA, USA).
CNS status
ND
ENA-FIRST-PUBLIC
2019-01-28T17:03:39Z
ENA-LAST-UPDATE
2018-11-30T09:11:08Z
External Id
SAMEA5144912
INSDC center name
Institute of Human Genetics Polish Academy of Sciences
INSDC first public
2019-01-28T17:03:39Z
INSDC last update
2018-11-30T09:11:08Z
INSDC status
public
Submitter Id
E-MTAB-7446:T1430
WBC at Dg
ND
age
2.6
broker name
ArrayExpress
cell type
T cell
common name
human
developmental stage
child
disease
T-cell acute lymphoblastic leukemia
individual
T1430
karyotype
ND
organism part
bone marrow
risk group
ND
sample name
E-MTAB-7446:T1430
scientific_name
Homo sapiens
sex
male
t-all immunophenotype
common
treatment protocol
ALL IC BFM 2009