4_1_GGCTAC_L008_R1_001.fastq.gz
Arabidopsis thaliana
Protocols: Arabidopsis thaliana seeds were surface sterilized with 20 ml thin bleach and 600 μL 37,5% HCl for 3 hours and then placed for 1.5 hour in laminar flow to evaporate chlorine gas. Seeds were stratified for 72 hours at 4°C and grown under long day conditions (21°C, 70% humidity, 16/8 light/dark cycle) in 100 ml liquid media containing 0.5xMurashi-Skoog basal salt, 0.5% sucrose, 1% M.E.S. monohydrate, pH 5.8 (KOH) with shaking (120 rpm). Ten days-old seedlings were treated with 150 mM NaCl in 0.1xMS media (salt stress) or 0.1xMS media (control) for time indicated separately per experiment. Seedlings were dried with paper towel and snap frozen in liquid nitrogen. 100 mg of tissue was ground and total RNA was extracted using Plant RNA extraction kit (Qiagen) according to manufacturer's instructions RNA quality determination, library preparation and sequencing with Illumina HiSeq 2500 was performed by Eurofins Genomics (Germany).
ENA-FIRST-PUBLIC
2019-06-30T04:02:41Z
ENA-LAST-UPDATE
2019-06-20T09:13:06Z
External Id
SAMEA5724031
INSDC center name
Plant Biology and Genome Center University of California, Davis
INSDC first public
2019-06-30T04:02:41Z
INSDC last update
2019-06-20T09:13:06Z
INSDC status
public
Submitter Id
E-MTAB-8073:4_1_GGCTAC_L008_R1_001.fastq.gz
age
10
broker name
ArrayExpress
common name
thale cress
developmental stage
seedling development stage
genotype
wild type genotype
growth condition
long day length regimen
organism part
whole organism
sample name
E-MTAB-8073:4_1_GGCTAC_L008_R1_001.fastq.gz
scientific_name
Arabidopsis thaliana
ecotype
Col-0