Sample 2
Mus musculus
Protocols: Embryonic Tbx20-GFP mouse hearts 11.5dpf were dissected and cross-linked in1.8% of formaldehyde for 10 minutes at room temperature. Samples were quenched by incubation with 2.5 M glycine for 5 minutes. Cells were washed and resuspended in Lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris, pH 8.1). The cross-linked chromatin was sonicated using a Misonix 4000 (amplitude 90 and 30 seconds on/30 seconds off) to an average size of 200 bp. Precleared chromatin extract was incubated overnight at 4AC with goat anti-GFP and immunoprecipitated with protein AaSepharose beads. 10 ng of DNA was used to generate a standard Illumina sequencing library. Enrichment of selected target regions was confirmed by qRT-PCR. Illumina's standard protocol was used
ENA-FIRST-PUBLIC
2016-06-30T17:01:22Z
ENA-LAST-UPDATE
2018-03-09T08:56:58Z
External Id
SAMEA3627869
INSDC center name
University of Chicago, Dept of Human Genetics
INSDC first public
2016-06-30T17:01:22Z
INSDC last update
2018-03-09T08:56:58Z
INSDC status
public
Submitter Id
E-MTAB-3967:Sample 2
broker name
ArrayExpress
common name
house mouse
developmental stage
embryonic day 11.5
organism part
whole heart
phenotype
wild-type
sample name
E-MTAB-3967:Sample 2
scientific_name
Mus musculus