GC058859.count
Homo sapiens
Protocols: Biopsies at the edge of an ulcer were taken during endoscopy prior to the start of therapy, stored in RNALater buffer (Ambion, Austin, TX, USA) and preserved at -80°C. Total RNA from inflamed biopsies was extracted using the AllPrep DNA/RNA Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The integrity and quantity of all RNA was assessed with a 2100 Bioanalyzer (Agilent, Waldbronn, Germany) and a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). Extracted RNA was stored at -80°C until further processing. Library preparation using the TruSeq Stranded mRNA protocol (Illumina, San Diego, USA) according to the manufacturer's instructions.
ENA-FIRST-PUBLIC
2019-01-24T17:03:14Z
ENA-LAST-UPDATE
2019-01-21T16:56:19Z
External Id
SAMEA5230454
INSDC center name
KU Leuven, Department of Chronic Diseases, Metabolism and Ageing
INSDC first public
2019-01-24T17:03:14Z
INSDC last update
2019-01-21T16:56:19Z
INSDC status
public
Submitter Id
E-MTAB-7604:GC058859.count
broker name
ArrayExpress
clinical history
non-responder
common name
human
developmental stage
adult
disease
inflammatory bowel disease
organism part
colon
sample name
E-MTAB-7604:GC058859.count
sampling site
ulcer
scientific_name
Homo sapiens
treatment
adalimumab