shoot_control_day1_rep2
Brassica napus
Protocols: Brassica napus root and shoot samples were collected on days one and five post-infection with P. aeruginosa. The biological replicates (n = 2) are the result of three pooled plant samples. The whole plant was sectioned in two at the root junction before the RNA was extracted. Brassica napus seeds were germinated on MS (Murashige and Skoog basal medium with vitamins from Phytotechnology Laboratories supplemented with 0.5 g L-1 MES hydrate and 0.5% sucrose at pH 5.7) agar plates for seven days. Seedlings were then transferred to a 50 mL conical tube with 5 mL MS media, and allowed to grow for three days. Seedlings were infected with Pseudomonas aeruginosa strain PA14, and tissue samples were taken on day one and five post-infection. Brassica napus tissue samples were separated, frozen using liquid nitrogen and grinded using a mortar and pestle. The RNA was extracted according to manufacturer's protocol using a RNeasy Plant Mini Kit (Qiagen). DNA was removed from all of the RNA samples using the DNA-free kit (Invitrogen™). Total RNA (1μg) was used for preparing the library with TrueSeq RNA sample Prep Kit v2 (Illumina®) according to manufacturer's guidelines with 24 different barcodes (two biological replicates).
ENA-FIRST-PUBLIC
2019-04-25T04:02:50Z
ENA-LAST-UPDATE
2019-04-15T11:51:54Z
External Id
SAMEA5567368
INSDC center name
Dalhousie University
INSDC first public
2019-04-25T04:02:50Z
INSDC last update
2019-04-15T11:51:54Z
INSDC status
public
Submitter Id
E-MTAB-7864:shoot_control_day1_rep2
age
10
broker name
ArrayExpress
common name
rape
developmental stage
seedling developmental stage
individual
mixed pool of 3 plants
organism part
shoot system
sample name
E-MTAB-7864:shoot_control_day1_rep2
scientific_name
Brassica napus