256
synthetic construct
Protocols: Sequencing ready synthetic constructs with STR sequences of varying length.The constructs where sequenced as is and following several PCR amplifications. See further details in \Short Tandem Repeat stutter model inferred from direct measurement of in vitro stutter noise\ (https://doi.org/10.1101/065110) The constructs that went through PCR amplification where barcoded in that PCR in a way that over-writes the original barcodes they where synthesized with.
# repeats
5
ENA-FIRST-PUBLIC
2019-01-26T17:02:10Z
ENA-LAST-UPDATE
2019-01-16T16:45:25Z
External Id
SAMEA5226760
INSDC center name
Department of Computer Science and Applied Mathematics, Weizmann Institute of Science
INSDC first public
2019-01-26T17:02:10Z
INSDC last update
2019-01-16T16:45:25Z
INSDC status
public
Submitter Id
E-MTAB-7602:256
broker name
ArrayExpress
cloning plasmid
Modified puc57-Kan* (GenScript)
final sequence
AGTACTAAGCAATGACAATGATACGGCGACCACCGAGATCTACACTCCCATGGACACTCTTTCCCTACACGACGCTCTTCCGATCTTCCTAATCTTACGCGGCCATAACcgtCGACTACCGTTGTCCATAGCGATAGGGGGTCCGCAGTAGTATTGGAGCCGTATGATGGGCTCTTAAAGACTGTCCATGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACTTCAATCTCGTATGCCGTCTTCTGCTTGCATTGCAGTACTAA
flanking sequence (left)
CGACTACCGTTGTCCATAGCGATA
flanking sequence (right)
TCCGCAGTAGTATTGGAGCCGTAT
genotype
5
identifier barcode
cgt
name of plasmid
SyMS_5G
repeat type
G
sample name
E-MTAB-7602:256
scientific_name
synthetic construct
sequencing primer fw (illumina)
ACACTCTTTCCCTACACGACGCTCTTCCGATCT
sequencing primer rev (illumina)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
str (ms) seq
GGGGG