Cup 2
Dictyostelium discoideum
Protocols: Cup cells were isolated using Fluorescent Activated Cell Sorting (FACS). Specifically, a gene fusion of YFP and the promoter of the late cup-specific gene DDB_G0278537 promoter was transformed into D. discoideum AX2 strain cells. TRIzol (Thermo Fisher) was used for extraction Libraries were prepared with Illumina Truseq stranded mRNA library preparation kit.
ENA-FIRST-PUBLIC
2019-01-31T17:02:55Z
ENA-LAST-UPDATE
2018-09-24T15:26:26Z
External Id
SAMEA4936731
INSDC center name
School of Life Sciences, University of Dundee
INSDC first public
2019-01-31T17:02:55Z
INSDC last update
2018-09-24T15:26:26Z
INSDC status
public
Submitter Id
E-MTAB-7215:Cup 2
age
24
broker name
ArrayExpress
cell type
cup cell
developmental stage
fruiting body stage
organism part
cup
sample name
E-MTAB-7215:Cup 2
scientific_name
Dictyostelium discoideum
strain
AX2