Sample 1
Saccharina latissima
Protocols: Young sporophytes of S. latissima were raised from stock cultures of clonal male and female gametophytes at the Alfred-Wegener-Institute Helmholtz Centre for Polar and Marine Research (AWI, Bremerhaven, Germany; stock culture #3 2013 Roscoff; 3123 and 3124 Spitsbergen) according to the protocol of Heinrich et al. (2012b). Vegetative gametophytes were isolated from spores of one fertile sporophyte collected at Kongsfjorden (79°N, 11°E; Spitsbergen, Norway) and at Roscoff (48° 43′ 39″ N, 3° 59′ 13.2″ W; Brittany, France), respectively (Bartsch 2018) Bartsch I. (2018). Derivation of clonal stock cultures and hybridization of kelps. In Protocols for Macroalgae Research (pp. 61-78): CRC Press Taylor and Francis Group Heinrich S, Valentin K, Frickenhaus S, John U, Wiencke C (2012) Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae). PLOS ONE, 7, e44342. Total RNA extraction was conducted using the method described in Heinrich et al. 2012. Heinrich S, Frickenhaus S, Glöckner G, Valentin K (2012a) A comprehensive cDNA library of light- and temperature-stressed Saccharina latissima (Phaeophyceae). European Journal of Phycology, 47, 83-94. https://www.tandfonline.com/doi/abs/10.1080/09670262.2012.660639 cDNA libraries were prepared with an Illumina TruSeq RNA Library Prep Kit according to the manufacturer protocol.
ENA-FIRST-PUBLIC
2019-01-31T17:02:08Z
ENA-LAST-UPDATE
2018-10-29T17:19:11Z
External Id
SAMEA5053883
INSDC center name
Marine Botany, Faculty Biology/Chemistry, University of Bremen, Bremen, Germany
INSDC first public
2019-01-31T17:02:08Z
INSDC last update
2018-10-29T17:19:11Z
INSDC status
public
Submitter Id
E-MTAB-7348:Sample 1
broker name
ArrayExpress
developmental stage
sporophyte development stage
ecotype
Roscoff
growth condition
temperature 8C/salinity SA 30
organism part
whole organism
sample name
E-MTAB-7348:Sample 1
scientific_name
Saccharina latissima