Stalk 1
Dictyostelium discoideum
Protocols: Stalk cells were isolated from 24 h fruiting bodies by pipetting vigorously in pronase/EDTA, followed by deposition on 50 µm nylon mesh and extensive washing with KK2. The stalks retained on the mesh were then collected, placed on top of 5 ml 25% Percoll in KK2 and centrifuged at 10,000g for 5 minutes at 4ºC. Stalks were collected from the top layer, washed with 5 volumes of KK2 and pelleted at 4,000 x g for 3 minutes. RNeasy kit (QIAGEN) was used for extraction Libraries were prepared with Illumina Truseq stranded mRNA library preparation kit.
ENA-FIRST-PUBLIC
2019-01-31T17:02:55Z
ENA-LAST-UPDATE
2018-09-24T15:26:26Z
External Id
SAMEA4936736
INSDC center name
School of Life Sciences, University of Dundee
INSDC first public
2019-01-31T17:02:55Z
INSDC last update
2018-09-24T15:26:26Z
INSDC status
public
Submitter Id
E-MTAB-7215:Stalk 1
age
24
broker name
ArrayExpress
cell type
stalk cell
developmental stage
fruiting body stage
organism part
stalk
sample name
E-MTAB-7215:Stalk 1
scientific_name
Dictyostelium discoideum
strain
AX2