Lck-49
Mus musculus
Protocols: RNA was isolated from the the small intestine of mice 6 days after they were transplanted with either wildtype allogeneic T cells or glucocorticoid-resistant allogeneic T cells (mouse model of acute graft-versus-host disease. Mice were sacrificed by carbon dioxide inhalation and snap frozen in liquid nitrogen. RNA was isolated from the the small intestine of 8-12 week-old mice 6 days after they were transplanted with either wildtype allogeneic T cells or glucocorticoid-resistant allogeneic T cells (mouse model of acute graft-versus-host disease. Mice were sacrificed by carbon dioxide inhalation and snap frozen in liquid nitrogen. mice were subjected to a total body irradiation with a dose of 8.5 Gy using an X-Ray source operated at 200 kV, 15 mA and 0.5-mm Cu filtration. The following day, 1x107 BM cells and 2x106 T cells (either wild-type or glucocorticoid resistant) were mixed and injected into the irradiated mice via the tail vein to induce acute graft-versus-host disease Total RNA was isolated using the RNeasy mini kit (qiagen) and poly adenylated RNA was isolated using oligo d(T) beads prior to reverse transcription Sequencing libraries were prepared using the TruSeq RNA library Prep Kit (Illumina)
ENA-FIRST-PUBLIC
2019-03-22T10:12:41Z
ENA-LAST-UPDATE
2019-03-12T11:09:37Z
External Id
SAMEA5417386
INSDC center name
MPIMG
INSDC first public
2019-03-22T10:12:41Z
INSDC last update
2019-03-12T11:09:37Z
INSDC status
public
Submitter Id
E-MTAB-7765:Lck-49
age
8 to 12
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
disease
acute graft vs. host disease
genotype
wild type genotype
organism part
small intestine
sample name
E-MTAB-7765:Lck-49
scientific_name
Mus musculus
strain
BALB/c