Whole Genome Sequence and Molecular Characterization of a Giant Temperate Phage Infecting Clostridium difficile
Clostridium phage phiCD211
Clostridium difficile ATCC9689 strain has been investigated for the presence of functional prophages. Following mitomycine C induction, a temperate phage termed φCD211 has been isolated. Transmission electron microscopy (TEM) has revealed a member of Siphoviridae family, with large icosahedral capsid and unusually long tail. Phage DNA has been purified directly from induction lysate and subjected to complete genome sequencing using Illumina technology. Following sequencing, double stranded-DNA (dsDNA) genome of 131,326 bp has been assembled and a putative function could be assigned to 60 of the 190 open reading frames. LC-MS/MS analysis of whole virion particles has enabled identification of 33 putative structural proteins. Bioinformatics analysis revealed the presence of multiple transposases along with putative horizontally acquired regions of bacterial origin. Additionally, an unusually high number (15) of transcriptional regulators / DNA binding proteins have been identified suggesting a complex regulation of DNA replication and gene transcription. To the best of our knowledge, φCD211 genome is the largest phage genomic sequence described so far.
ENA first public
2015-02-12
ENA last update
2016-10-21
External Id
SAMEA2757648
INSDC center alias
PASTEUR
INSDC center name
Institut Pasteur, FRANCE
INSDC first public
2015-02-12T17:06:47Z
INSDC last update
2016-10-21T09:44:55Z
INSDC status
public
Submitter Id
phiCD2111
collection date
2012
geographic location (country and/or sea)
Canada
host health state
disease
host scientific name
Clostridioides difficile
isolation source
laboratory
sample name
phiCD2111
serovar
ATCC9689
strain
Clostridium difficile