Sequences of on- and off-target genomic sites from Hek293T cells treated with ATM sequences from Hek293 cells treated with evolved  L3-2 TALENsand control sgRNAs
Homo sapiens
Sequencing from Hubbard et al, 2015.  293 cells were transfected with a plasmid expressing homodimeric FokI fusions to the evolved L3-2 TALE together with a plasmid encoding the canonical ATM-R TALEâFokI fusion protein. After 48 h, genomic DNA was harvested and the ATM target site and its corresponding known off-target sites OffA11 and OffA17 were amplified from this genomic DNA.  The amplified sites werethen  high-throughput sequenced.  All genomic target sites (ATM on-target, OffA11 and OffA17) were combined together for this one type of TALEN treatment.
isolate
Hek293
age
?
biomaterial_provider
GenTarget
sex
female
tissue
Hek293
TALEN targets
ATM
genomic sites amplifed
ATM on-target, ATM OffA11, ATM OffA18
on-target sites
yes
off-target sites
yes
BioSampleModel
Human