PUF3 RNAi +tet 1
Trypanosoma brucei
Protocols: RNA was made from whole cells Cells were exponentially growing Addition of 100ng/ml tetracycline 24h before RNA purification Standard Trizol follwed by rRNA depletion using oligonucleotides and RNaseH Illumina kit
ENA-FIRST-PUBLIC
2019-06-30T04:02:40Z
ENA-LAST-UPDATE
2019-06-14T15:31:51Z
External Id
SAMEA5718567
INSDC center name
University of Heidelberg Center for Molecular Biology (ZMBH)
INSDC first public
2019-06-30T04:02:40Z
INSDC last update
2019-06-14T15:31:51Z
INSDC status
public
Submitter Id
E-MTAB-8062:PUF3 RNAi +tet 1
broker name
ArrayExpress
cell line
EATRO1125
developmental stage
bloodstream form
genotype
Tet repressor, V5-PUF3, stem-loop RNAi targeting PUF3
growth condition
100 ng/ml tetracycline 24h
organism part
whole organism
phenotype
PUF3 knockdown
sample name
E-MTAB-8062:PUF3 RNAi +tet 1
sampling time
24
scientific_name
Trypanosoma brucei