Spore 1
Dictyostelium discoideum
Protocols: To isolate spores, 24 h fruiting bodies were collected, dissociated and sieved through 50 µm nylon mesh as described above. The flow-through containing spores was passed next through 35 µm nylon mesh, and incubated with 0.1% Triton X-100 for 5 minutes to remove amoeboid cells. RNeasy kit (QIAGEN) was used for extraction Libraries were prepared with Illumina Truseq stranded mRNA library preparation kit.
ENA-FIRST-PUBLIC
2019-01-31T17:02:55Z
ENA-LAST-UPDATE
2018-09-24T15:26:26Z
External Id
SAMEA4936733
INSDC center name
School of Life Sciences, University of Dundee
INSDC first public
2019-01-31T17:02:55Z
INSDC last update
2018-09-24T15:26:26Z
INSDC status
public
Submitter Id
E-MTAB-7215:Spore 1
age
24
broker name
ArrayExpress
cell type
spore cell
developmental stage
fruiting body stage
organism part
spore
sample name
E-MTAB-7215:Spore 1
scientific_name
Dictyostelium discoideum
strain
AX2