Sample 1
Mus musculus
Protocols: Hearts were harvested in icecold PBS with 0.5 mM EDTA and dissociated as described (Van Handel et al. 2012), using collagenase (10 mg/ml; Worthington) and dispase (10 mg/mL, Invitrogen). Cells were stained for 30 minutes, 4',6-diamidino-2-phenylindole (DAPI, 1:1000) was used to label dead cells. Tie2Cre lineage cells, negative for blood markers CD41, CD45 and Ter119, were sorted on an InfluxTM cell sorter (BD Biosciences) and collected in RNA lysis buffer (Agilent) or TRIzol reagent (Ambion) for RNA extraction, or PBS/5% serum for ATAC-Seq (Buenrostro et al. 2013). Van Handel B, Montel-Hagen A, Sasidharan R, Nakano H, Ferrari R, Boogerd CJ, Schredelseker J, Wang Y, Hunter S, Org T, et al. 2012. Scl represses cardiomyogenesis in prospective hemogenic endothelium and endocardium. Cell 150: 590605. Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. 2013. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods 10: 12138. We performed ATAC-seq according to Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. 2013. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods 10: 12138.
ENA-FIRST-PUBLIC
2016-06-30T17:01:22Z
ENA-LAST-UPDATE
2018-03-09T09:21:22Z
External Id
SAMEA3627870
INSDC center name
University of Chicago
INSDC first public
2016-06-30T17:01:22Z
INSDC last update
2018-03-09T09:21:22Z
INSDC status
public
Submitter Id
E-MTAB-3972:Sample 1
broker name
ArrayExpress
cell type
endocardial
common name
house mouse
date
2014-09-26
developmental stage
embryonic day 12.5
phenotype
wild-type
replicate
1
sample name
E-MTAB-3972:Sample 1
scientific_name
Mus musculus