Seawater sample Sylt, 2012-08-15
seawater metagenome
seawater sample taken near the oyster reef at low tide; temperature and salinity measured at a nearby site by ship Mya IIl
ENA first public
2016-06-29
ENA last update
2016-10-21
External Id
SAMEA3462816
INSDC center alias
ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
INSDC center name
ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
INSDC first public
2016-06-29T17:01:56Z
INSDC last update
2016-10-21T09:44:06Z
INSDC status
public
Submitter Id
SW_Sylt_20120815
ammonium
0.824
chlorophyll
4.3
collection date
2012-08-15
dissolved organic nitrogen
22.0354
environment (biome)
aquatic, marine, benthic, coastal
environment (feature)
oyster reef
environment (material)
environmental material, water, seawater
geographic location (country and/or sea)
North Sea
geographic location (depth)
0.5
geographic location (latitude)
55.02919
geographic location (longitude)
8.434186
investigation type
mimarks-survey
multiplex identifiers
f: ACCAGTTG; r: ACAGCTCA
nitrate
0.06
nitrite
0.009
pcr conditions
initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
pcr primers
27f-338r
pcr primers
27F: TCAGAGTTTGATCMTGGCTCAG + 338r: CATGCTGCCTCCCGTAGGAGT
ph
8.16
phosphate
0.18
project name
PRJEB9624
salinity
29.76609269
sample name
SW_Sylt_20120815
sample volume or weight for DNA extraction
100
sequencing method
Sequencing by synthesis, Illumina paired-end
silicate
0.63
suspended particulate matter
1.2
target gene
16s rRNA
target subfragment
V1-V2
temperature
19.983
total dissolved nitrogen
22.68
water environmental package
water