NS5_Ascl1
Mus musculus
Protocols: NS5 cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 NS5 cells were infected with pBABE-Ascl1-ERT2-IRES-GFP-puro (NS5_Ascl1), followed by selection in complete medium containing 1ug/mL puromycin (Calbiochem), and collected 30 minutes after induction with 4-Hydroxytamoxifen at a final concentration of 50nM, while reducing EGF concentration to 5ng/ml NS cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with rabbit anti-HA (ab1424, Abcam) DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol.
ENA-FIRST-PUBLIC
2015-02-16T17:01:57Z
ENA-LAST-UPDATE
2018-03-08T17:13:33Z
External Id
SAMEA2395623
INSDC center name
Instituto Gulbenkian de Ciencia
INSDC first public
2015-02-16T17:01:57Z
INSDC last update
2018-03-08T17:13:33Z
INSDC status
public
Submitter Id
E-MTAB-2384:NS5_Ascl1
broker name
ArrayExpress
cell line
HS5
cell type
neural stem cell
common name
house mouse
sample name
E-MTAB-2384:NS5_Ascl1
scientific_name
Mus musculus