mRNA-seq, 24h RA-induction Danio embryos, uninjected, replicate3
Danio rerio
mRNA-seq, 20h RA-induction Danio embryos, uninjected, replicate3
strain
AB
age
20 hrs post-fertilization
dev_stage
Embryonic
sex
pooled male and female
tissue
whole embryo
biomaterial_provider
Diane Slusarski Lab, University of Iowa
cell_subtype
wild-type
growth_protocol
Fertilized AB (wild-type) zebrafish eggs were microinjected with only the reporter construct containing GFP as described previously.
sample_type
whole organism
label
mRNA-seq, 20h RA-induction Danio embryos, uninjected, replicate3
treatment
At least 150 GFP positive embryos were collected in Trizol. RNA was extracted using Directzol ( Zymo Research , R2070) as per manufacturer’s instruction. Poly-A selected directional TruSeq libraries were prepared according to manufacturer''s specifications.
biological_replicate
3
BioSampleModel
Model organism or animal