Sample 3
Mus musculus
Protocols: NS5 cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 NS5 cells were infected with pBABE-Ascl1-ERT2-IRES-GFP-puro (NS5_Ascl1), followed by selection in complete medium containing 1ug/mL puromycin (Calbiochem), and then collected for chromatin extraction NS cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with antibodies anti-H3K27Ac and anti-H3K4me1 (ab4729 and ab8895 from Abcam). DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol.
ENA-FIRST-PUBLIC
2015-02-16T17:02:01Z
ENA-LAST-UPDATE
2018-03-08T22:17:23Z
External Id
SAMEA3121481
INSDC center name
Instituto Gulbenkian de Ciencia
INSDC first public
2015-02-16T17:02:01Z
INSDC last update
2018-03-08T22:17:23Z
INSDC status
public
Submitter Id
E-MTAB-3104:Sample 3
broker name
ArrayExpress
cell line
NS5
cell type
neural progenitor cell
common name
house mouse
genotype
Ascl1-ERT2
phenotype
Ascl1-ERT2 expressed, but inactive
sample name
E-MTAB-3104:Sample 3
scientific_name
Mus musculus