HDFn-KLF4_dNp73b_empty-d6-RNAseq-1
Homo sapiens
HDFn cells were grown in Medium 106 (Thermo Fisher Scientific, M-106-500) to 33-66% confluency in 6-well plates and spinfected (1 hour at 2000 rpm) with KLF4 + ∆Np63α + ∆Np73β lentivirus (3X copy number of each). The spinfection procedure was repeated 24 hours later and fresh media was added at 48 hours. Cells were grown for 6 days after the initial infection, collected by trypsinization, and frozen at -20°C. Total RNA was isolated for RNA-seq analysis from the cell pellet using the RNAqueous-Micro Total RNA Isolation Kit (Thermo Fisher Scientific, AM1931). Samples were treated with DNase I for 20 minutes at room temperature.
isolate
not applicable
age
neonatal
biomaterial_provider
ATCC (PCS-201-010)
sex
male
tissue
foreskin
cell_line
not applicable
cell_type
primary human dermal fibroblasts (HDF)
sample_type
poly-A RNA
replicate
1
treatment
KLF4 + ∆Np63α + ∆Np73β
treatment_dose
3X
treatment_time
6 days
BioSampleModel
Human