NG-7876_ML_RI_lib64742_3388_8_1.fastq.bz2
Brachypodium distachyon
Protocols: Tissue samples were collected from eight distinct developmental stages; pre-anthesis ovaries, young grains (1-3 DAA), middle length grains (3-8 DAA), full length grains (8-15 DAA), mature grains (15-20 DAA), mature grains without embryo, germinating grains and seedlings at 3-4 days after germination (Fig.1). Tissues were frozen in liquid nitrogen immediately after collection and were kept at -80°C until further analyses. Brachypodium distachyon grains (Bd-21 accession) were germinated on moist filter paper after stratification at 4°C for 48h. Five-day old seedlings were transferred to 9cm square pots with a 2:2:1 multipurpose compost: vermiculite: sand mix and grown under controlled environment conditions with a 18h photoperiod at 20°-22°C and light intensity of 180-200 umol/m/s. Total RNA was isolated from frozen tissue samples using the Spectrum Plant Total RNA Kit (Sigma-Aldrich) following the manufacturer's instructions. Three biological replicates were used for each stage of development. Random primed cDNA libraries were created and sequenced by GATC Biotech (https://www.gatc-biotech.com).
ENA-FIRST-PUBLIC
2019-01-31T17:02:09Z
ENA-LAST-UPDATE
2019-01-21T14:22:02Z
External Id
SAMEA5230269
INSDC center name
Associate Professor Department of Genetics and Genome Biology
INSDC first public
2019-01-31T17:02:09Z
INSDC last update
2019-01-21T14:22:02Z
INSDC status
public
Submitter Id
E-MTAB-7607:NG-7876_ML_RI_lib64742_3388_8_1.fastq.bz2
age
3- 8 days after anthesis
broker name
ArrayExpress
common name
stiff brome
cultivar
BD-21
developmental stage
middle length grains
genotype
wild type genotype
organism part
plant ovule
replicate
biological replicate
sample name
E-MTAB-7607:NG-7876_ML_RI_lib64742_3388_8_1.fastq.bz2
scientific_name
Brachypodium distachyon