Pacific oyster hemolymph microbiota from Sylt
Magallana gigas
Pacific oyster (Crassostrea gigas) hemolymph microbiota; collected on Sylt, 2012-06-21; sample taken after field deployment; treated with mix of ampiciline, gentamicine, tetracycline and kanamycine (100 µg/l seawater each) between 2012-05-29 and 2012-06-01; oyster from Sylt, not translocated
ENA first public
2016-06-29
ENA last update
2016-10-21
External Id
SAMEA3461890
INSDC center alias
ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
INSDC center name
ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
INSDC first public
2016-06-29T17:01:56Z
INSDC last update
2016-10-21T09:39:29Z
INSDC status
public
Submitter Id
Cg_55_Hem_Sy_SyA_20120621
amount or size of sample collected
0.002
chemical administration
Mix of ampiciline, gentamicine, tetracycline and kanamycine, each 100 µg/l seawater each
collection date
2012-06-21
common name
Pacific oyster
environment (biome)
aquatic, marine, benthic, coastal
environment (feature)
organic
environment (material)
organic material, bodily fluid
geographic location (country and/or sea)
North Sea
geographic location (latitude)
55.02924483
geographic location (longitude)
8.434046257
geographic location (region and locality)
Schleswig Holstein, Sylt, List, Odewatt
host body habitat
hemolymph
host body site
adductor muscle
host common name
Pacific oyster
host life stage
adult
host subject id
Sy_55
host taxid
29159
host-associated environmental package
host-associated
investigation type
mimarks-survey
multiplex identifiers
f: GGCCATAT; r: GCTTGGAT
pcr conditions
initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
pcr primers
27f-338r
pcr primers
27F: TCAGAGTTTGATCMTGGCTCAG + 338r: CATGCTGCCTCCCGTAGGAGT
perturbation
treated with mix of ampiciline, gentamicine, tetracycline and kanamycine (100 µg/l seawater each) between 2012-05-29 and 2012-06-01
project name
PRJEB9624
sample collection device or method
syringe
sample name
Cg_55_Hem_Sy_SyA_20120621
sample storage temperature
-80
sample volume or weight for DNA extraction
0.2
sequencing method
Sequencing by synthesis, Illumina paired-end
target gene
16s rRNA
target subfragment
V1-V2
temperature
15.092