Pacific oyster hemolymph microbiota from Sylt
Magallana gigas
Pacific oyster (Crassostrea gigas) hemolymph microbiota; collected on Sylt, 2012-06-21; sample taken after field deployment; not antibiotic treated; oyster from Sylt, not translocated
ENA first public
2016-06-29
ENA last update
2016-10-21
External Id
SAMEA3461647
INSDC center alias
ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
INSDC center name
ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
INSDC first public
2016-06-29T17:01:55Z
INSDC last update
2016-10-21T09:39:22Z
INSDC status
public
Submitter Id
Cg_111_Hem_Sy_SyC_20120621
amount or size of sample collected
0.002
collection date
2012-06-21
common name
Pacific oyster
environment (biome)
aquatic, marine, benthic, coastal
environment (feature)
organic
environment (material)
organic material, bodily fluid
geographic location (country and/or sea)
North Sea
geographic location (latitude)
55.02867221
geographic location (longitude)
8.434814011
geographic location (region and locality)
Schleswig Holstein, Sylt, List, Odewatt
host body habitat
hemolymph
host body site
adductor muscle
host common name
Pacific oyster
host life stage
adult
host subject id
Sy_111
host taxid
29159
host-associated environmental package
host-associated
investigation type
mimarks-survey
multiplex identifiers
f: GACACTCA; r: GCTTGGAT
pcr conditions
initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
pcr primers
27f-338r
pcr primers
27F: TCAGAGTTTGATCMTGGCTCAG + 338r: CATGCTGCCTCCCGTAGGAGT
perturbation
not antibiotic treated
project name
PRJEB9624
sample collection device or method
syringe
sample name
Cg_111_Hem_Sy_SyC_20120621
sample storage temperature
-80
sample volume or weight for DNA extraction
0.2
sequencing method
Sequencing by synthesis, Illumina paired-end
target gene
16s rRNA
target subfragment
V1-V2
temperature
15.092