mRNA-seq, 24h RA-induction Danio embryos, Hoxa1-3FlagMyc construct, replicate2
Danio rerio
mRNA-seq, 20h RA-induction Danio embryos, Hoxa1-3FlagMyc construct, replicate2
strain
AB
age
20 hrs post-fertilization
dev_stage
Embryonic
sex
pooled male and female
tissue
whole embryo
biomaterial_provider
Diane Slusarski Lab, University of Iowa
cell_subtype
wild-type
growth_protocol
Hoxa1-3Xflag-Myc RNA cDNA was cloned in pCS2+ vector. Clones with cDNA in right orientation were digested with Not1 and invitro transcription reaction were performed using mMESSAGE mMACHINE SP6 Transcription Kit (ThermoFisher scientific AM1340). Hoxa1-3Xflag-Myc RNA (25ng/ul) were co-microinjected into fertilized AB (wild-type) zebrafish eggs with a reporter construct containing GFP as described previously.
sample_type
whole organism
label
mRNA-seq, 20h RA-induction Danio embryos, Hoxa1-3FlagMyc construct, replicate2
treatment
At least 150 GFP positive embryos were collected in Trizol. RNA was extracted using Directzol ( Zymo Research , R2070) as per manufacturer’s instruction. Poly-A selected directional TruSeq libraries were prepared according to manufacturer''s specifications.
biological_replicate
2
BioSampleModel
Model organism or animal