In planta genome editing
Zea mays
Cas9 and single guide RNA DNA expression cassettes targeting 3 chromosomal locations were delivered to immature maize embryos. Loci where DNA cleavage was expected was PCR amplified and deep sequenced to identify Cas9-guide RNA induced mutations.
cultivar
Hi-Type II
dev_stage
Immature embryo
geo_loc_name
USA: Iowa
tissue
Transformed immature embryo
biomaterial_provider
8305 NW 62nd Ave
BioSampleModel
Plant