Sample 497
Mus musculus
Protocols: For ESCs, cells were maintained as described previously in NDiff 227 media containing 2i inhibitors (CHIR99021 and PD0325901) on dishes coated with gelatin (Millipore, ES-006-B) (Yang et al., 2012). For d1EpiLCs and d2EpiLCs, cells were cultured in NDiff N2B27 media containing 12ng/ml bFGF; 20 ng/ml activin A and 1% KnockOut Serum Replacement on dishes coated with 5 μg/ml bovine plasma fibronectin (Hayashi et al., 2011). Cells have a heterozygote knock-in unstable GFP at Rex1 locus (see Yang et al 2014). not applicable ICell8 single-cell RNA-seq system (Goldstein et al, 2017)
ENA-FIRST-PUBLIC
2019-01-31T17:02:06Z
ENA-LAST-UPDATE
2018-09-17T20:38:03Z
External Id
SAMEA4921093
INSDC center name
The University of Manchester
INSDC first public
2019-01-31T17:02:06Z
INSDC last update
2018-09-17T20:38:03Z
INSDC status
public
Submitter Id
E-MTAB-7211:Sample 497
broker name
ArrayExpress
cell line
ES-E14TG2a.4
cell type
epiblast-like cell
common name
house mouse
developmental stage
embryo
genotype
Rex1-GFPd2
organism part
inner cell mass
post-analysis single cell quality
pass
progenitor cell type
embryonic stem cell
sample name
E-MTAB-7211:Sample 497
scientific_name
Mus musculus
strain
129 mouse strain