LSK_WT_normal_2_F
Mus musculus
Protocols: Ctnnb1fl/fl mice were crossed with Rosa26-CreER Rosa26-YFP mice to generate Ctnnb1fl/fl RosaCre-ER RosaYFP mice. These mice were crossed with Hoxa9-/- knockout mice to generate Hoxa9-/- Ctnnb1fl/fl RosaCre-ER RosaYFP mice. Compound homozygous animals were used for experiments. For leukemia development experiments, C57BL/6 or SJL mice were given 11Gy total body Îł-irradiation and injected with up to 500,000 test cells mixed with 200,000 C57BL/6 or SJL bone marrow nuclear cells. LSK (lin-, Sca-1+, c-Kit+) and GMP (lin-, Sca-1-, c-Kit+, CD34+, CD16/32lo) were isolated from lineage negative cells after lineage (Gr-1, Mac-1, B220, Ter119, CD3e, CD4, CD8) depletion using a BD FACS ARIA cell sorter. 300ng to 1ug of mIRVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina).
ENA-FIRST-PUBLIC
2016-06-30T17:01:22Z
ENA-LAST-UPDATE
2018-03-09T01:42:12Z
External Id
SAMEA3460546
INSDC center name
'Eric So lab Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King''s College London'
INSDC first public
2016-06-30T17:01:22Z
INSDC last update
2018-03-09T01:42:12Z
INSDC status
public
Submitter Id
E-MTAB-3647:LSK_WT_normal_2_F
broker name
ArrayExpress
cell type
hematopoietic stem cell
common name
house mouse
disease
normal
genotype
wild type genotype
individual
H2
sample name
E-MTAB-3647:LSK_WT_normal_2_F
scientific_name
Mus musculus