Sequences of on- and off-target genomic sites from Hek293T cells treated with ATM sequences from Hek293 cells treated with controland control sgRNAs
Homo sapiens
Sequencing from Hubbard et al, 2015.  293 cells were transfected with a control plasmid. After 48 h, genomic DNA was harvested and the ATM target site and its corresponding known off-target sites OffA11 and OffA17 were amplified from this genomic DNA.  The amplified sites werethen  high-throughput sequenced.  All genomic target sites (ATM on-target, OffA11 and OffA17) were combined together for this one type of TALEN treatment.
isolate
Hek293
age
?
biomaterial_provider
GenTarget
sex
female
tissue
Hek293
TALEN targets
ATM
genomic sites amplifed
ATM on-target, ATM OffA11, ATM OffA19
on-target sites
yes
off-target sites
yes
BioSampleModel
Human