GmMYB176-OE 1
Glycine max
Protocols: Soybean Transgenic hairy roots were selected using a GFP marker under a Leica MZ FL III fluorescence stereomicroscope with a GFP filter. To obtain soybean cotyledons for hairy root transformation, soybean (Glycine max L. Merr.) cv Harosoy63 seeds were grown. Six-day-old soybean cotyledons were harvested and transformed with A. rhizogenes K599 carrying silencing (pGmMYB176-Si) or overexpressing (pGmMYB176-OE) constructs. RNA was extracted from four pooled replicates (100 mg each) of GmMYB176-OE, GmMYB176-Si and control soybean hairy root samples using RNeasy plant mini kit (Qiagen, USA). The RNA quality and concentration were checked in Agilent Bioanalyzer (Agilent, USA). TruSeq RNA sequencing libraries were constructed following the standard preparation guide (Illumina, USA).
ENA-FIRST-PUBLIC
2019-01-29T17:02:03Z
ENA-LAST-UPDATE
2018-11-07T16:38:08Z
External Id
SAMEA5071874
INSDC center name
Research Associate London Research and Development centre Agriculture and Agri-Food Canada
INSDC first public
2019-01-29T17:02:03Z
INSDC last update
2018-11-07T16:38:08Z
INSDC status
public
Submitter Id
E-MTAB-7379:GmMYB176-OE 1
age
6
broker name
ArrayExpress
common name
soybean
cultivar
Harasoy63
developmental stage
cotyledon emergence stage
genotype
GmMYB176-OE
infect
Agrobacterium rhizogenes strain K599
organism part
root
phenotype
GmMYB176 overexpression
sample name
E-MTAB-7379:GmMYB176-OE 1
scientific_name
Glycine max