Anlotinib_resistant_NCI-H1975_control_GTGGCC_L003_R1.fastq
Homo sapiens
Protocols: The cells were cultured in RPMI 1640 medium (Gibco, USA) supplement with 10% FBS (Gibco, USA), and 0.1 mg/ml streptomycin, and 100 U/ml penicillin. All cells were incubated at 37℃, 5% CO2 in a humidified incubator. Cells cultured in 10 cm diametre dish. 1 ml trizol reagent (Life Technologies, Inc., USA) was used to lyse cell samples. Total RNA isolation using standard procedures.Oligotex mRNA Mini Kit (Qiagen, Germany) was performed to collect pure mRNA. About 100 ng mRNA of each sample was used to library. The library procedure includes cDNA reverse-transcription, end repair, ligation, and PCR amplification.
ENA-FIRST-PUBLIC
2019-07-01T04:02:33Z
ENA-LAST-UPDATE
2018-07-25T09:34:38Z
External Id
SAMEA4803054
INSDC center name
Shanghai Chest Hospital, Shanghai Jiao Tong University
INSDC first public
2019-07-01T04:02:33Z
INSDC last update
2018-07-25T09:34:38Z
INSDC status
public
Submitter Id
E-MTAB-7068:Anlotinib_resistant_NCI-H1975_control_GTGGCC_L003_R1.fastq
broker name
ArrayExpress
cell line
NCI-H1975
cell type
epithelial cell
common name
human
disease
non-small cell lung adenocarcinoma
organism part
lung
phenotype
Anlotinib-resistance
sample name
E-MTAB-7068:Anlotinib_resistant_NCI-H1975_control_GTGGCC_L003_R1.fastq
scientific_name
Homo sapiens