T1886
Homo sapiens
Protocols: T-cells (T-ALL and normal T lymphocytes) were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson). Total RNA was isolated using miRCURY RNA Isolation Kit Cell & Plant (Exiqon). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). Libraries were generated with NEBNext® Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, MA, USA).
CNS status
1
ENA-FIRST-PUBLIC
2019-01-28T17:03:39Z
ENA-LAST-UPDATE
2018-11-30T09:11:08Z
External Id
SAMEA5144905
INSDC center name
Institute of Human Genetics Polish Academy of Sciences
INSDC first public
2019-01-28T17:03:39Z
INSDC last update
2018-11-30T09:11:08Z
INSDC status
public
Submitter Id
E-MTAB-7446:T1886
WBC at Dg
91 400
age
14
broker name
ArrayExpress
cell type
T cell
common name
human
developmental stage
child
disease
T-cell acute lymphoblastic leukemia
individual
T1886
karyotype
46,XY, der(1)t(1;18)(p36;q21), der(5)t(5;9)(q11.2;p22),der(9)t(5;9)(p12;q132), der(9)del(9)(q21)t(9;?;q12),der(11)del(11)(q13)t(9;?;11)(p22;?;q12), der(11)del(11)(q12)t(5;11)(p13;q12)+13, der(16)del(16)(q12)?t(16;?18)(?p11.2), ider(?)t(?;5)(?;13)[17]// 49, XY, idem, dupl(11)(q22q23)[11]//
organism part
bone marrow
risk group
IR
sample name
E-MTAB-7446:T1886
scientific_name
Homo sapiens
sex
male
t-all immunophenotype
pre
treatment protocol
ALL IC BFM 2009