mina-1_YA_1
Caenorhabditis elegans
Protocols: All C. elegans strains were maintained and raised at 20°C on NGM agar seeded with Escherichia coli OP50 (Brenner, 1974). RNA extraction was done using TRIzol reagent (Life Technologies) in accordance with the manufacturer's protocol and additionally purified by a solution of acidic phenol:chloroform (5:1 v/v) pH 4.5 (Ambion) followed by a second chloroform extraction to obtain high-grade RNA for sequencing. Finally, DNA traces were degraded by DNAse I (Ambion). Random primed cDNA library was generated from each sample by GATC Biotech.
ENA-FIRST-PUBLIC
2019-01-31T17:02:08Z
ENA-LAST-UPDATE
2018-11-09T16:23:22Z
External Id
SAMEA5078363
INSDC center name
University Children's Hospital Basel, University of Basel, Spitalstrasse 33, 4056 Basel, Switzerland
INSDC first public
2019-01-31T17:02:08Z
INSDC last update
2018-11-09T16:23:22Z
INSDC status
public
Submitter Id
E-MTAB-7389:mina-1_YA_1
age
68
broker name
ArrayExpress
developmental stage
adult
genotype
mina-1/C41G7.3(ok1521) I
organism part
whole organism
sample name
E-MTAB-7389:mina-1_YA_1
scientific_name
Caenorhabditis elegans
sex
mixed
strain
VC1054