HDFn-empty-d6-RNAseq-1
Homo sapiens
HDFn cells were grown in Medium 106 (Thermo Fisher Scientific, M-106-500) to 33-66% confluency in 6-well plates and spinfected (1 hour at 2000 rpm) with empty vector control lentivirus (9X total copy number). The spinfection procedure was repeated 24 hours later and fresh media was added at 48 hours. Cells were grown for 6 days after the initial infection, collected by trypsinization, and frozen at -20°C. Total RNA was isolated for RNA-seq analysis from the cell pellet using the RNAqueous-Micro Total RNA Isolation Kit (Thermo Fisher Scientific, AM1931). Samples were treated with DNase I for 20 minutes at room temperature.
isolate
not applicable
age
neonatal
biomaterial_provider
ATCC (PCS-201-010)
sex
male
tissue
foreskin
cell_line
not applicable
cell_type
primary human dermal fibroblasts (HDF)
sample_type
poly-A RNA
replicate
1
treatment
empty vector control
treatment_dose
3X
treatment_time
6 days
BioSampleModel
Human