biocrust metagenome
host
not applicable
isolation_source
biological soil crust
collection_date
2012-04-27
geo_loc_name
Germany: Lieberose
lat_lon
51.55 N 14.22 E
samp_mat_process
DNA was extracted using the 'Genomic DNA from soil' NucleoSpin Soil Kit (Macherey-Nagel, DE). DNA was sheared using an E220 Focused-ultrasonicator (Covaris, USA). Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit for Illumina and the NEBNext Multiplex Oligos for Illumina (both New England Biolabs, UK). Sequencing was performed on a MiSeq sequencer using a MiSeq Reagent Kit v3 for 600 cycle (Illumina, USA).
source_material_id
L13
BioSampleModel
Metagenome or environmental