4B0_draft genome
Lactococcus cremoris
strain
4B0
collection_date
2017-11-04
env_biome
Plants and animal habitats
env_feature
Laboratory isolate
env_material
Bacterial culture
geo_loc_name
India: New Delhi
isol_growth_condt
The strain was isolated from the continuous stirred tank reactor running at 4% isobutanol containing modified GM17 media. The strain was further grown in GM17 media in a static flask at 30 degrees for 24 - 48 hours and Its genome was isolated using the standard genomic DNA isolation protocol for gram positive bacteria.
lat_lon
28.5402 N 77.1662 E
num_replicons
Plasmid cured strain
ref_biomaterial
Not available
biotic_relationship
free living
encoded_traits
antibiotics resistance traits are present
estimated_size
2.53 Mb
extrachrom_elements
Plasmids absent
health_state
Mutant 4B0 srain was obtained after evolution experiments
isolation_source
4% isobutanol tolerant sttrain was obtained after evolution experiments performed on wild type NZ9000 L. lactis strain.
pathogenicity
No
rel_to_oxygen
facultative
samp_collect_device
Microfuge tubes
samp_mat_process
Samples from the static flasks were collected, centrifuged and immediately processed for genomic DNA isolation.
samp_size
2 ml
source_material_id
4B0
subspecf_gen_lin
Lactococcus lactis subspecies cremoris
BioSampleModel
MIGS.ba