Snyder_NHR-77_GFP_L1_rep2_GFP_TGCT
Caenorhabditis elegans
source_name
Snyder_NHR-77_GFP_L1_rep2_GFP_TGCT
strain
OP353(official name : OP353 genotype : unc119(ed3); wgIs353(nhr-77::TY1 EGFP FLAG; unc119) outcross : 3 transgene : nhr-77 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-77::EGFP fusion protein is expressed in the correct nhr-77 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-77 transcription factor. made_by : R Waterston )
developmental stage
fed L1
genotype
unc119(ed3);wgIs353(nhr-77::TY1 EGFP FLAG;unc119)
Sex
hermaphrodite
transgene
nhr-77