Environmental/Metatranscriptome sample from lab-reared Spodoptera frugiperda larvae guts from Tucuman province (Argentina)
insect gut metagenome
collection_date
2010-12-23/2010-12-28
env_biome
anthropogenic terrestrial biome (ENVO:01000219). A laboratory Spodoptera frugiperda colony was established from field specimens collected from a maize (Zea mays) field belonging to the Tucuman National University in El Manantial locality (Lules department, Tucuman province; 495 above sea level; 26 49 50 S; 65 16 59,4 W). Collected larvae were then reared for 9 generations under controlled photoperiod, temperature and humidity conditions on an artificial diet made of white bean, essential nutrients and preservatives, without the addition of antibiotics, adapted from Murua and Virla (2003).
env_feature
cultivated habitat (ENVO:00000113). Larvae were originally collected from a maize (Zea mays) field belonging to the Tucuman National University in El Manantial locality (Lules department, Tucuman province)
env_material
environmental material (ENVO:00010483), anthropogenic environmental material(ENVO:0010001), food product(ENVO:00002002). Collected larvae were reared under controlled photoperiod, temperature and humidity conditions on an artificial diet made of white bean, essential nutrients and preservatives, without the addition of antibiotics, adapted from Murua and Virla (2003).
geo_loc_name
Argentina: Tucuman province, Lules department, El Manantial locality
host
Spodoptera frugiperda
lat_lon
26.49 S 65.16 W
chem_administration
The diet included the following chemicals: ascorbic acid, sorbic acid, methyl parahydroxybenzoate and formaldehyde.
elev
495 above sea level
host_age
5th instar
host_body_habitat
gut
host_diet
Artificial diet composed of (per Kg): 600 g ground white beans, 140 g wheat germ, 120 g brewers' yeast, 160g agar, 27 g ascorbic acid, 8 g sorbic acid, 8 g Nipagin M (methyl parahydroxybenzoate), 0.008 lts 40% formaldehyde, 3.8 lts distilled water
host_growth_cond
Evaluacion de cuatro dietas artificiales para la cria de Spodoptera frugiperda (Lep.: Noctuidae) destinada a mantener poblaciones experimentales de himenopteros parasitoides (2003) Murua and Virla. Bol. San. Veg. Plagas (29) 43-51.
host_life_stage
larvae
host_tissue_sampled
gut
samp_mat_process
Total RNA extraction
samp_store_temp
-80C
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.host-associated