Improving Soil Bacterial Taxa-Area Relationships Assessment Using DNA Meta-Barcoding
soil metagenome
ENA first public
2014-08-28
ENA last update
2016-10-21
External Id
SAMEA2732805
INSDC center alias
INRA-CMSE
INSDC center name
UMR Agroecology, INRA - Burgundy University, Dijon, France
INSDC first public
2014-08-28T17:01:20Z
INSDC last update
2016-10-21T09:33:20Z
INSDC status
public
Submitter Id
101265C17
collection date
2003
depth
0.3
environment (biome)
terrestrial biome
environment (feature)
anthropogenic geographic feature
environment (material)
soil
geographic location (country and/or sea)
France
geographic location (elevation)
358
geographic location (latitude)
46.3852824165
geographic location (longitude)
4.40393982583
investigation type
mimarks-survey
multiplex identifiers
CGTGTCTCTA
pcr conditions
5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation : 94degC_2 min; 35 cycles of denaturation : 94degC_30sec; annealing: 52degC_30sec; elongation: 72degC_1min; final elongation: 72degC_7min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5' position to specifically identify each sample and avoid PCR biases
pcr primers
F479 (5'-CAGCMGCYGCNGTAANAC-3') R888 (5'-CCGYCAATTCMTTTRAGT-3')
project name
4REG_bacteria_soil_diversity
sample name
101265C17
sequencing method
454-pyrosequencing
soil environmental package
soil
target gene
16S rRNA gene