Wt2 in vivo
Salmonella enterica subsp. enterica serovar Typhimurium
source_name
Wild-type
strain
wildtype
tissue
CT26 tumor infected with S. Typhimurium
treatment protocol
Tumor development: 5*10^5 CT26 cells were intradermally injected into the right flank of a BALB/c mouse. After the the tumors reached a size of approx. 150 mm³, the mice were infected i.v. with 5*10^6 Salmonella. RNA Extraction from CT26 tumors (Bielecki et al.. PLoS One 2011): Two days post-infection (p.i.) mice were necrotized and infected tumors prepared for stabilization of bacterial RNA in the following way: the tumors were removed and excised into 2–4 pieces, put onto nylon filters for cell culture (70 µm pore size) and suspended in 2 ml of RNAproctect Bacteria Reagent in 2 cm petri dishes. The single tumors were spent through the membrane with a sterile spatula. The resulting mixture in the RNAprotect reagent was collected and centrifuged for 5 min at maximum speed 16,000 x g. The pellet was immediately frozen at −70°C for further RNA extraction.
serovar
Typhimurium