H3K4me3_ICeChIP-seq_HEK293_2
Homo sapiens
source_name
Cell culture
cell line
HEK 293
chip antibody
H3K4me3 (Active Motif, 39159, lot 1609004)
growth protocol
HEK293 cells were grown in 37°C, 5% CO2, 95% humidity in high glucose DMEM (Invitrogen) supplemented with 10% (v/v) HyClone FBS Characterized U.S. and 1X Penicillin/Streptomycin (Gibco). HEK293 cultures were grown in 15 cm polystyrene dishes, passaged 1:20 every 5 days, Cells used in ChIP experiments were harvested when 80-90% confluent with media changed at least 3 hours prior harvesting.
amplification cycles
12
map quality cut-off
13
mononucleosome cut-off
not performed
reference genome
hg19