marine metagenome
collection_date
2016-01-25
depth
30
env_broad_scale
marine biome
env_local_scale
pelagic
env_medium
seawater
geo_loc_name
Indian Ocean: Southern Ocean
lat_lon
61.97 S 92.548 E
diss_oxygen
361.12 μmol/L
nitrate
22.901 μmol/L
phosphate
1.36 μmol/L
pressure
30 dbar
salinity
33.6873 PSU
samp_collect_device
CTD Rosette/Niskin bottle
samp_mat_process
filtered with 0.2 micron filter then stored at -80°C
samp_size
2 litres
samp_store_temp
-80°C
silicate
37 μmol/L
temp
0.8668
seq_meth
Illumina MiSeq
target_gene
16S rRNA
target_subfragment
V1-V3
pcr_primers
27F - 519R
nucl_acid_ext
https://data.bioplatforms.com/dataset/7bd9dd00-f234-4a8b-9698-570da3e647d9/resource/a9dd8e40-92c5-4323-9a41-0b4ce8231855/download/Pelagic_sampling_SOP_16122016.pdf
pcr_cond
95°C, 10 min - [94°C, 30 sec - 55°C, 10 sec - 72°C, 45 sec] x 35 cycles - 72°C, 10 min - 4°C, Hold
seq_quality_check
manually edited
chimera_check
unoise3 - USEARCH v11
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.water