IB2WHZR01__Monbetsu3V4V5__V4V5_f
marine metagenome
sample_name
DRS012364
env_biome
Temperate shelf and sea biome
collection_date
2012-04-23T11:00:00+09:00
env_feature
marine habitat
geo_loc_name
Japan:Monbetsu-shi,Kaiyokoen
lat_lon
44.337 N 143.380833 E
env_material
sea water
project_name
Monbetsu18S
depth
0 meter
env_package
water
investigation_type
metagenome
sample comment
1 L of surface seawaters were taken by a plastic bucket on Apr. 23 in 2012 from Okhotsk Sea (Monbetsu city, Japan) (44d20.22'N, 143d22.85'E). To trap all plankton components appeared in the seawaters, 500 mL of the seawaters were filtrated on 8 um pore-size polycarbonate filters (nuclepore membrane, GE Healthcare, Tokyo, Japan), and seawaters which passed through 8 um filter were further filtrated on 1 um pore-size (GE Healthcare). The filters were stored at a deep freezer (-80 degrees Celsius) until use.
submitted_to_insdc
true
target_gene
18S rRNA
seq_meth
pyrosequencing