H3_ICeChIP-seq_HEK293
Homo sapiens
source_name
Cell culture
cell line
HEK 293
chip antibody
H3 (Active Motif, 61277, lot 32412002)
growth protocol
HEK293 cells were grown in 37°C, 5% CO2, 95% humidity in high glucose DMEM (Invitrogen) supplemented with 10% (v/v) HyClone FBS Characterized U.S. and 1X Penicillin/Streptomycin (Gibco). HEK293 cultures were grown in 15 cm polystyrene dishes, passaged 1:20 every 5 days, Cells used in ChIP experiments were harvested when 80-90% confluent with media changed at least 3 hours prior harvesting.
amplification cycles
9
map quality cut-off
0
mononucleosome cut-off
not performed
reference genome
hg19