Quantseq_ST-Dox_MSX2_iOX_r1
Homo sapiens
source_name
Quantseq_ST-Dox_MSX2_iOX
cell type
hTSC-derived syncytiotrophoblast
differentiation state
syncytiotrophoblast (ST)
modification
inducible MSX2
dox induction
no dox
modification protocol
To generate the inducible MSX2 expression construct, the coding sequence of MSX2 including the 3xFlag tag was cloned into PiggyBac-Tre-Dest-rTA-HSV-neo. After transfection, the hTSC were selected with 300 μg/ml G418 and expression was induced with 1 μg/ml doxycycline.