lake water metagenome
isolation_source
lake water
collection_date
2018-07-20
geo_loc_name
Russia:Lake Baikal
lat_lon
51.50036 N 104.24401 E
samp_collect_device
Shale Niskin
samp_mat_process
Water samples (5 L) were filtered on policarbonate filters (25 mm diameter, 0.2 micron pore size; Millipore, Germany) using a peristaltic pump. The filter was then placed in a TE buffer (10 mM Tris-HCl, pH 7.4; 1 mM EDTA, pH 8.0) and frozen at -20. Then, it was transported to the laboratory. DNA was extracted according to the modified method of phenol-chloroform extraction (Sambrook et al. 1989). The extracted DNA was kept at -70C for the further use.
samp_size
5L
BioSampleModel
Metagenome or environmental