Snyder_SPTF-1_GFP_L1_rep1_GFP_GTAT
Caenorhabditis elegans
source_name
Snyder_SPTF-1_GFP_L1_rep1_GFP_GTAT
strain
OP196(official name : OP196 genotype : unc119(ed3); wgIs196(sptf1::TY1 EGFP FLAG C; unc119) outcross : 3 transgene : sptf-1 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The SPTF-1::EGFP fusion protein is expressed in the correct sptf-1 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the SPTF-1 transcription factor. made_by : S Kim )
developmental stage
fed L1
genotype
unc119(ed3);wgIs196(sptf1::TY1 EGFP FLAG C;unc119)
Sex
hermaphrodite
transgene
sptf-1