Lolium multiflorum
isolate
SVA02
geographic location
Brazil:Pelotas
tissue
leafs
age
30 days
library_ID
SVA02_1_3
title
Lolium multiflorum Lam biovar SVA02 (glyphosate-suceptible) treated with glyphosate (replicate 3)
library_strategy
RNA-Seq
library_source
TRANSCRIPTOMIC
library_selection
RANDOM
library_layout
paired
platform
ILLUMINA
instrument_model
Illumina HiSeq 2500
design_description
The plants were treated with glyphosate at a dose of 2160 g ea. ha-1. Leaf samples from individual plants were harvested 24 hours after application. The sample was centrifuged again at 11700rpm for 15min at 4oC for pellet formation, followed by rapid vortexing. The RNA pellet was carefully decanted from the supernatant and immediately washed in cold 75% alcohol solution, followed by centrifugation at 7500rpm for 5min at 4oC and redissolved in 20L of DEPC water. The amount and quality of the RNA samples were evaluated on a 2% agarose gel and spectrophotometer (Nanovue Plus 1000) before sequencing on the Illumina (Hi-Seq2500 System) platform.
filetype
fastq
filename
11_R1_001.fastq
filename2
11_R2_001.fastq
BioSampleModel
Plant