Human lymphatic vessel cells laser-dissected from RNAlater preserved jejunum sample
Homo sapiens
We report a robust protocol to effectively maintain high RNA quality for immuno-fluorescence-based laser capture microdissection (LCM), enabling finely resolved spatial transcriptome acquisition with only tens of cells from snap-frozen or RNAlater-treated tissues. Here about 100 podoplanin labeled lymphatic cells were laser captured from the RNAlater preserved human jejunum sample and conducted RNAseq.
isolate
this jejunum was collected from a gastrectomy pateint
age
80
biomaterial_provider
RenJi Hospital
sex
male
tissue
jejunum
cell_type
lymphatic vessel cells
disease
gastirc cancer
ethnicity
Chinese
health_state
disease
sample_type
tissue sample
treatment
stored in RNAlater, frozen section and laser-dissected
BioSampleModel
Human