Sample 1
Mus musculus
Protocols: Spleens were harvested and were perfused with RPMI 1640 medium containing 2% FCS, 20 mM Hepes (all from Lonza), 0.2 mg/ml Collagenase P (Sigma Aldrich), 0.8 U/ml Dispase I (Sigma Aldrich) and 100 µg/ml DNaseI (Applichem) with 22G a syringe. Samples were teared into smaller pieces and incubated at 37°C for 30 minutes, with resuspension and collection of supernatant every 15 min to PBS containing 1% FCS and 10 mM EDTA (MACS buffer). To enrich fibroblastic stromal cells, hematopoietic and erythrocytes were depleted by incubating the cell suspension with MACS anti-CD45 and anti-TER119 microbeads (Miltenyi Biotec) and passing them through a MACS LS column (Miltenyi Biotec). ICAM1+/PDPN+/CD140b+/CD157+ cells from Ccl19-iEYFP adult spleens were sorted with a Bio-Rad S3 cell sorter and pooled together. Single cell suspensions were run on the 10x Chromium (10X Genomics). The cDNA library generation is performed by functional genomic center Zurich following the established commercial protocol for Chromium Single Cell 3' Reagent Kit (v2 Chemistry) (Catalogue Number PN-120237).
ENA-FIRST-PUBLIC
2019-03-27T17:05:15Z
ENA-LAST-UPDATE
2019-02-21T09:03:15Z
External Id
SAMEA5364686
INSDC center name
Institute of Immunobiology, Kantonsspital St. Gallen
INSDC first public
2019-03-27T17:05:15Z
INSDC last update
2019-02-21T09:03:15Z
INSDC status
public
Submitter Id
E-MTAB-7703:Sample 1
age
6 to 8
broker name
ArrayExpress
cell type
fibroblast
common name
house mouse
developmental stage
adult
genotype
C57BL/6N-Tg(Ccl19-tTA)xLC1xR26-EYFP
individual
2 mice pooled
organism part
spleen
sample name
E-MTAB-7703:Sample 1
scientific_name
Mus musculus
sex
mixed
strain
C57BL/6NJ