Mutagenized HAP1 Tet-On-MLKLTE/SD cells untreated
Homo sapiens
Protocols: The selection experiment was performed by inducing MLKLTE/SD expression with 200 ng/mL DOX over five days and subsequently allowing resistant colonies to grow for four days after withdrawing DOX. Surviving colonies were collected using trypsin detachment. For the unselected sample cells were left untreated and cells were collected using trypsin. Nucleic acid was extracted using Qiagen columns ( QIAamp DNA Mini Kit; catalog # 51306) Nucleic acid library construction as performed as described before (PMID: 21623355)
ENA-FIRST-PUBLIC
2018-05-16T17:03:08Z
ENA-LAST-UPDATE
2018-05-11T10:09:32Z
External Id
SAMEA4659244
INSDC center name
STANFORD UNIVERSITY
INSDC first public
2018-05-16T17:03:08Z
INSDC last update
2018-05-11T10:09:32Z
INSDC status
public
Submitter Id
E-MTAB-6777:Mutagenized HAP1 Tet-On-MLKLTE/SD cells untreated
broker name
ArrayExpress
cell line
HAP1
common name
human
disease
chronic myelogenous leukemia
genotype
Tet-On-MLKLTE/SD
growth condition
unselected
sample name
E-MTAB-6777:Mutagenized HAP1 Tet-On-MLKLTE/SD cells untreated
scientific_name
Homo sapiens