Control replica 2
Phaseolus vulgaris
Plasmids were introduced into Agrobacterium rhizogenes K599 strain, and used for plant transformation in independent experiments with 15 plants each. Plants were maintained for hairy root formation for 2 weeks and we obtained from 2 to 6 independent TdT fluorescence positive roots per plant, which were pooled in each independent experiment used for total RNA isolation.
cultivar
Pinto Saltillo
ecotype
missing
age
2 weeks
geo_loc_name
Mexico: Cuernavaca, Morelos
tissue
transgenic hairy roots
biomaterial_provider
Dr. Jose Luis Reyes, Instituto de Biotecnología, UNAM
collected_by
Biol. Guadalupe Sosa-Valencia
collection_date
2014-10
growth_protocol
Estrada-Navarrete G, Alvarado-Affantranger X, Olivares JE, Guillen G, Diaz-Camino C, Campos F, Quinto C, Gresshoff PM, Sanchez F. 2007. Fast, efficient and reproducible genetic transformation of Phaseolus spp. by Agrobacterium rhizogenes. Nat Protoc 2, 1819-1824.
sample_type
hairy roots
temp
24 C
BioSampleModel
Plant