Prokaryote
gut metagenome
Stomach content samples requested from the Pacific Marine Specimen Bank (www.spc.int).
host
Katsuwonus pelamis & Thunnus albacares
isolation_source
Stomach content of tuna species captured in the Pacific ocean
collection_date
2015/2017
geo_loc_name
Pacific Ocean
lat_lon
not applicable
ref_biomaterial
www.spc.int
samp_collect_device
Each stomach was taken out of the freezer, defrosted and cut open using a longitudinal incision. Then, undigested carcasses and hard parts were removed and placed aside in a sterile container. The stomach lining was then scraped using the unsharpened side of a sterile scalpel and carefully placed inside a sterile 50 mL falcon tube. Following, the stomach lining was briefly washed with MilliQ water using a squirt bottle and mixed with the stomach gastric juices. Approximately 5-25 mL of stomach liquid was then collected using a 1 mL disposable pipette and placed inside the same 50 mL falcon tube as the lining scraped sample.
samp_mat_process
Amplicons obtained from the prokaryote primers.
samp_size
55 skipjack (Katsuwonus pelamis) and 41 yellowfin (Thunnus albacares)
BioSampleModel
Metagenome or environmental