Revealing the Transcriptomic Complexity of Switchgrass by PacBio Long-Read Sequencing
Panicum virgatum
ENA-FIRST-PUBLIC
2018-05-27T17:02:47Z
ENA-LAST-UPDATE
2018-05-13T00:41:03Z
External Id
SAMEA4662974
INSDC center name
Department of Biochemistry and Molecular Biology and Institute of Bioinformatics
INSDC first public
2018-05-27T17:02:47Z
INSDC last update
2018-05-13T00:41:03Z
INSDC status
public
Submitter Id
Switchgrass PacBio FL transcript
collection date
2014
common name
switchgrass
estimated size
10G
geographic location (country and/or sea)
USA
geographic location (latitude)
40
geographic location (longitude)
30
isolation and growth condition
Total RNA of the above ground tissues was isolated using RNeasy Plant Mini Kit. Total RNA of roots was isolated from roots using TRI REAGENT
number of replicons
1
plant developmental stage
R1 developmental stage
plant growth medium
a 16-h light photoperiod (6:00 am-10:00 pm) with supplementary lighting from parabolic aluminized reflector lamps (average 390 μE/m2/S1), and relative humidity 77-22% (average 51%). The temperature in the greenhouse ranged from 25°C to 29 °C (average 26 °C). Plants were watered three times per week, and fertilizer (Peter's Fert 20-10-20, 100 ppm) was applied during the last watering each week.
plant structure
A whole tiller consisting of leaves, leaf sheaths, internodes, nodes and flowers and roots were collected
sample name
Switchgrass PacBio FL transcript
scientific_name
Panicum virgatum