R18L6I27-35-43 human GTFs ChIP-exo
Homo sapiens
Human cell lines were grown exponentially at 37C at 5% CO2 in DMEM supplemented with 10% bovine calf serum, then             subjected to 1% formaldehyde crosslinking for 10 min at 25C. Cells were harvested, lysed, and the purified chromatin was             sonicated by standard methods. With the following modifications, ChIP-exo was carried out as previously described (Rhee and             Pugh, 2012) with chromatin extracted from 10 million cells (HepG2, ATCC), ProteinG MagSepharose resin (GE Healthcare), and 3 ug             of TFIIB (Santa Cruz Biotech, sc-225).