RNA_Seq_LUs23
Homo sapiens
Protocols: Cells were not treated with siRNA (untreated), treated with anti-STAU1 siRNA (knockdown), or treated with anti-STAU1 siRNA and the STAU1 level was rescued by the rescue expression of siRNA-resistant STAU1 (rescue). Cells were subjected to Cycloheximide treatment before collection. RNA-Seq was performed in parallel with ribosome profiling. The protocol was followed to isolate RNA from the Cycloheximide teated cells and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction as described in the manuscript. Random barcodes were also incoperated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina paired-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
ENA-FIRST-PUBLIC
2015-03-01T17:02:25Z
ENA-LAST-UPDATE
2018-03-08T20:24:07Z
External Id
SAMEA2771249
INSDC center name
Department of Molecular Neuroscience, UCL Institute of Neurology
INSDC first public
2015-03-01T17:02:25Z
INSDC last update
2018-03-08T20:24:07Z
INSDC status
public
Submitter Id
E-MTAB-2940:RNA_Seq_LUs23
broker name
ArrayExpress
cell line
HEK293
common name
human
sample name
E-MTAB-2940:RNA_Seq_LUs23
scientific_name
Homo sapiens