MEF_deltaSp2
Mus musculus
Protocols: MEFs were maintained at 37 degrees celcius in 50 % DMEM High Glucose and 50 % Ham's F10 Medium (PAA) supplemented with 10% fetal bovine serum (PAA) and 1X Penicillin and Streptomycin (PAA). ChIP-qPCR experiments were performed essentially as described previously (Stielow et al., 2008, see below) using the OneDay ChIP kit (Diagenode) according to the manufacturer's instructions. Briefly, MEFs and HEK293 cells were incubated with 1% formaldehyde for 10 min at room temperature followed by quenching with 150 mM glycine for 5 min. After sonification, 100 microgram of chromatin was used for single immunoprecipitations using 2 microgram of self-made Sp2 antibody (Baur et al., 2010). Ten immunoprecipitations were pooled for ChIP-seq and purified by the QIAquick PCR Purification kit (Qiagen). 10 ng of inmunoprecipitated DNA fragments were subjected to paired-end sequencing on an Illumina IIx Genome Ananlyzer. Stielow, B., et al. (2008). SUMO-modified Sp3 represses transcription by provoking local heterochromatic gene silencing. EMBO Rep 9, 899-906.
ENA-FIRST-PUBLIC
2013-02-21T17:02:27Z
ENA-LAST-UPDATE
2018-03-08T15:38:19Z
External Id
SAMEA1710140
INSDC center name
Institute for Molecular Biology and Tumor Research
INSDC first public
2013-02-21T17:02:27Z
INSDC last update
2018-03-08T15:38:19Z
INSDC status
public
StrainOrLine
C57BL/6N
Submitter Id
E-MTAB-994:MEF_deltaSp2
broker name
ArrayExpress
cell line
mouse embryonic fibroblasts
common name
house mouse
genotype
deltaSp2
sample name
E-MTAB-994:MEF_deltaSp2
scientific_name
Mus musculus