Ostrinia nubilalis 454 transcriptome
Ostrinia nubilalis
The aim of this study was to create transcriptome resources for the lepidopter sibling species Ostrinia scapulalis (adzuki bean borer) and O. nubilalis (European corn borer). This BioProject sample entry holds the raw and assembled data of the O. nubilalis transcriptome. O. nubilalis samples were collected on maize in northern France. RNA was extracted from a pool of heads and thorax of four adult moths (two males and two females) and postprocessed. The synthesized cDNA was normalized by one cycle of denaturation and reassociation. After separation of reassociated ds-cDNA the remaining ss-cDNA was amplified with 13 PCR cycles. The normalized cDNA library (range of 500-700bp) was sequenced in one GS FLX Titanium (Roche 454) standard chemistry run (1/4 plate). The raw sequences were cleaned (adapter sequences, species barcodes, ribosomal sequences and too short sequences were removed) and then assembled with Newbler (v. 2.5.3).
strain
European corn borer
sex
pooled male and female
collection_date
2009
geo_loc_name
France: northern, Loos en Gohelle
age
larval stage
dev_stage
larval stage
specific_host
29057
isolation_source
sampled on maize
tissue_type
head and thorax