Mercenaria mercenaria
The clams sampled from field were acclimatized to seawater at 15 °C for a week before air exposure.Clams were exposed to air in a multi-thermo incubator (Eyela,MTI-202) with temperature and humidity set as 15 ℃ and 60% respectively. At the beginning (0 day) and 8th day after air exposure, live hard clams were sampled. Each time point had three replicates(three individuals per replicated) .For each replicate, the gills was aseptically dissected and pooled, followed by RNA extraction and sequencing.
isolate
None
breed
None
host
None
isolation_source
None
collection_date
2018-05-01/2018-05-09
geo_loc_name
China: Dongying
tissue
Gill
age
4
altitude
Not applicable
biomaterial_provider
Not applicable
collected_by
Hao Song
depth
Not applicable
dev_stage
Adult
env_biome
Not applicable
host_tissue_sampled
Not applicable
identified_by
Not applicable
lat_lon
37.25 N 118.55 E
sex
pooled male and female
specimen_voucher
Not applicable
temp
15℃
Investigation_type
eukaryote
Classification
Animalia; Mollusca; Bivalvia; Veneroida; Veneridae; Mercenaria; Mercenaria Mercenaria
Environment
Air exposure
BioSampleModel
Invertebrate