Sample 8
Mus musculus
Protocols: The full-length mRNA of mouse Klf1(NM-010635) was cloned into the pMIG retroviral vector, which contains an IRES-GFP cassette. Splenocytes from C57BL/6 mice were cultured for 48 h in the presence of Concanavalin A (ConA) (10 μg/mL) and IL-2 (50 μg/mL), and gene transduction was conducted. CD4+ T cells that were transfected with retroviral vectors were divided into 3 fractions according to GFP fluorescence (strongly positive, weakly positive and negative) using a FACSVantage flow cytometer. Total RNA was extracted using ｍRNA extraction kit(RNeasy Micro Kit, QIAGEN) Sequence libraries were prepared according to standard methods using Smart-seq2 (Illumina)
ENA-FIRST-PUBLIC
2018-05-10T17:03:12Z
ENA-LAST-UPDATE
2018-05-09T10:44:04Z
External Id
SAMEA4653167
INSDC center name
Department of Allergy and Rheumatology, Graduate School of Medicine, The University of Tokyo
INSDC first public
2018-05-10T17:03:12Z
INSDC last update
2018-05-09T10:44:04Z
INSDC status
public
Submitter Id
E-MTAB-6778:Sample 8
age
8
broker name
ArrayExpress
cell type
CD4-positive helper T cell
common name
house mouse
disease
normal
genotype
pMIG-Mock_GFP
organism part
spleen
phenotype
empty vector; low GFP expression
sample name
E-MTAB-6778:Sample 8
scientific_name
Mus musculus
sex
female
strain
C57BL/6