Pool Talinum fruticosum tissues
Talinum fruticosum
cultivar
#5.1.7.9.5 (a selfed laboratory population)
dev_stage
seedlings, roots, mature leaves, flowers
geo_loc_name
not applicable
tissue
pool of tissues, including seedlings, roots, flowers and leaves from a drought-stressed plant or treated with exogenous abscisic acid (ABA)
sample_type
pool of tissue samples
Plant material and growth conditions
Seeds of Talinum triangulare obtained after five subsequent, controlled self-pollination events were germinated in D 400 soil with Cocopor (Stender) and grown in a controlled-environment plant chamber (MobyLux GroBanks, CLF Plant Climatics) under the following conditions: 12 h light/12 h dark at 25 C/23 C. The light intensity at the leaf level was 150200 mol s1 m2. In parallel, a batch of the same seed stock was sterilised by washing twice in 70% ethanol for 15 min followed by two washes with sterile water (15 min each) and germinated on Murashige and Skoog (MS) medium with 3% sucrose.
Tissue type details
The following samples were harvested by snap-freezing respective tissues in liquid nitrogen: seedlings grown on soil harvested 6 to 12 after sowing, roots of plants grown in soil in which case roots were washed in water and rinsed with 70 % ethanol prior to freezing, roots of plants grown in vitro on MS medium (25 days after sowing), young leaves grown under control conditions (21 days after sowing), mature leaves harvested 160 min and 640 min after foliar application of 200 M abscisic acid (ABA) for the presence of ABA-inducible transcripts (40 days after sowing), whole leaves harvested at the end of day and at the end of night after water withdrawal for seven days for presence of drought-responsive and/or CAM-related transcripts (40 days after sowing), and whole flowers harvested continuously (53 to 70 days after sowing).
BioSampleModel
Plant