B6_PBS_3h
Mus musculus
Protocols: The mice were euthanized at different time points post exposure (at 3, 8, 24, 48 and 72 hours); Eight week old female C57 Black mice were fasted for 24 hours prior to a single intraperitoneal injection of 350mg/kg of acetaminophen in phosphate buffer saline (PBS) (treatment group) or PBS (control group) Liver and plasma samples were collected from different time points after mice had been exposed to acetaminophen. The plasma was prepared from EDTA blood by centrifugation at 1000 x g for 15 minutes to separate the plasma and blood cells. Total RNA from liver and plasma was extracted with the miRNeasy kit (Qiagen, Germantown, MD), as described. The quality and quantity of the RNA were evaluated with Agilent 2100 Bioanalyzer (Santa Clara, CA) and NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE). we constructed a single library with an insert size ranging from 20 to 100 nucleotides long. We also did not conduct mRNA purification and fragmentation steps prior to library construction since we did not observe ribosomal RNA peaks from plasma RNA on the bioanalyzer, and most of the transcripts in circulation were probably not full-length based on prior studies. This approach allowed us to obtain a general profile for both miRNA and mRNA in the plasma from a single library.
ENA-FIRST-PUBLIC
2013-03-08T17:02:32Z
ENA-LAST-UPDATE
2018-03-08T16:18:59Z
External Id
SAMEA1713776
INSDC center name
Institute for Systems Biology
INSDC first public
2013-03-08T17:02:32Z
INSDC last update
2018-03-08T16:18:59Z
INSDC status
public
Submitter Id
E-MTAB-1538:B6_PBS_3h
age
8 week
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
organism part
plasma
phenotype
control
sample name
E-MTAB-1538:B6_PBS_3h
scientific_name
Mus musculus
specimen with known storage state
fresh specimen
strain
C57BL/6J