Sample 13
Sungouiella intermedia
Protocols: Samples were collected to follow growth, carbon source consumption and product formation. The yeast strain was cultivated in a DasGip system, using 4 reactors per condition. The different conditions were prepared with standard approach to grow yeast in reactors. The following conditions were used: Carbon source. (g/L) Dissolved oxygen (percentage): Glucose (30) <10%; Glucose (10) + Xylose (20) <10%; Glucose (20) 30-40%; Glucose (200) 30-40%; Xylose (20) 30-40%; Xylose (200) 30-40%; Lactose (20) 30-40%; Galactose (20) 30-40%; Cellobiose (20) 30-40% TRIzol (also known as TRI REAGENT) for the isolation of total RNA. TRIzol is a mixture of guanidine thioacyanate and phenol, which effectively dissolves DNA, RNA and protein on homogenization or lysis of tissue sample. After adding chloroform and centrifuging, the mixture separates into 3 phases with the upper clear aqueous phase containing the RNA. The next steps include transfer of the clear upper phase to 1 volume of absolute ethanol, and then transfer the mixture to an RNeasy column, following the kit instructions. TrueSeq
ENA-FIRST-PUBLIC
2018-06-29T17:01:50Z
ENA-LAST-UPDATE
2018-04-17T13:58:21Z
External Id
SAMEA4589533
INSDC center name
National Bioinformatics Infrastructure Sweden, Science for Life Laboratory, Uppsala University
INSDC first public
2018-06-29T17:01:50Z
INSDC last update
2018-04-17T13:58:21Z
INSDC status
public
Submitter Id
E-MTAB-6670:Sample 13
broker name
ArrayExpress
genotype
wild type genotype
growth condition
glucose 30 grams per liter; less than 10 percent dissolved oxygen
sample name
E-MTAB-6670:Sample 13
scientific_name
[Candida] intermedia
strain
CBS 141442