ZmAN3-GSyellow_1
Zea mays
Protocols: Leaf samples were always taken during steady state growth of the leaf (2d after the appearance of leaf4). Samples of four centimeter were pooled, encompassing the division zone, transition zone and the expansion zone. For each maize sample, 1.2 g leaf tissue (leaf 4 cm1-4) was fixed in Buffer A (Morohashi et al., 2012) for 15 min under vacuum conditions. For quenching, glycine was added to a final concentration of 0.1 M and incubated for 10 min. Fixed leaf tissue was washed with distilled water and frozen in liquid N2. ChIP-seq on PPD2-GSyellow and ZmAN3-GSyellow was performed using 5 L ab290 anti-GFP antibody (Abcam), as described in Morohashi et al., 2012. ChIP-seq libraries were prepared by GATC Biotech
ENA first public
2018-08-07
ENA last update
2018-02-21
External Id
SAMEA104605989
INSDC center alias
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
INSDC center name
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
INSDC first public
2018-08-07T17:03:06Z
INSDC last update
2018-02-21T16:05:02Z
INSDC status
public
Submitter Id
E-MTAB-6527:ZmAN3-GSyellow_1
age
2 days after emergence of leaf 4
broker name
ArrayExpress
cultivar
B104
genotype
ZmAN3-GSyellow
organism part
leaf
sample name
E-MTAB-6527:ZmAN3-GSyellow_1
sampling site
basal 4 cm of growing leaf 4