General Sample for Glycine max
Glycine max
Isoclark seed were germinated on paper for five to seven days at 27°C.  Following germination, seedlings were moved to a hydroponics bucket containing iron sufficient  (100 µM Fe(NO3)3•9H2O nutrient solutions. Nutrient solutions were based on growth conditions described in Chaney et al. (1992), with solutions adjusted for 10 L buckets. Fully expanded unifoliates of two Isoclark seedlings were rub-inoculated with empty vector Bean Pod Mottle Virus. Leaf tissue was harvest 21 days later. RNA was isolated individual samples and then pooled.
cultivar
Isoclark
label
MAG22