Vehicle 101
Mus musculus
Protocols: Adult mice were treated with AngII, PE, or vehicle for 3 days using in vivo mini pumps. Hearts were excised and myocytes were isolated. Total RNA was collected from isolated myocytes. Total RNA was extracted from isolated myocytes using RNeasy Fibrous Mini Tissue Kit (Qiagen) Total RNA was used as input, and oligo-dT purification to enrich for mRNA, followed by random-primed reverse-transcription, second-strand cDNA synthesis, ligation of indexed adaptors for Illumina sequencing, and subsequent library creation from the resulting double-stranded DNA.
ENA-FIRST-PUBLIC
2018-05-12T17:02:50Z
ENA-LAST-UPDATE
2018-05-08T09:26:52Z
External Id
SAMEA4647083
INSDC center name
UMENT
INSDC first public
2018-05-12T17:02:50Z
INSDC last update
2018-05-08T09:26:52Z
INSDC status
public
Submitter Id
E-MTAB-6770:Vehicle 101
age
10 to 11
broker name
ArrayExpress
cell type
myocyte
common name
house mouse
genotype
wild type genotype
organism part
heart
sample name
E-MTAB-6770:Vehicle 101
scientific_name
Mus musculus
sex
male
strain
FVB/NJ