iCLIP_STAU1_LUs14
Homo sapiens
Protocols: A FlpIn293 cell line expressing 3xFLAG-STAU1 was subjected to UV-C irradiation before collection. hiCLIP or iCLIP protocol was followed to isolate RNA bound to 3XFLAG-STAU1 protein and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction, and are specified in the manuscript. Random barcodes were also incorporated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina paired-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
ENA-FIRST-PUBLIC
2015-03-01T17:02:25Z
ENA-LAST-UPDATE
2018-03-08T20:21:13Z
External Id
SAMEA2771240
INSDC center name
Department of Molecular Neuroscience, UCL Institute of Neurology
INSDC first public
2015-03-01T17:02:25Z
INSDC last update
2018-03-08T20:21:13Z
INSDC status
public
Submitter Id
E-MTAB-2937:iCLIP_STAU1_LUs14
broker name
ArrayExpress
cell line
HEK293
common name
human
sample name
E-MTAB-2937:iCLIP_STAU1_LUs14
scientific_name
Homo sapiens