Sample 5
Mus musculus
Protocols: Mouse ureters were extracted from embryonic stage 15 mice and then cultured in serum-free defined media according to an established protocol (PMID: 24260555 and PMID: 18776146). Mouse experiments were approved by the University of Manchester ethics committee and the UK Home Office (licence PAFFCI44F). Mouse ureters were extracted from embryonic stage 15 mice and then cultured in serum-free defined media according to an established protocol (PMID: 24260555 and PMID: 18776146). There were 4 treatment conditions. Controls were grown in basal media or in the presence of 5 nanogram per milliliter recombinant human transforming growth factor-beta (TGFB1) for 1 day or 6 days. Each n=1 in the RNA-sequencing analyses comprised RNA extracted from the a set of six to eight ureters from the same litter grown in one condition for one or six days. Comparisons (n=3 for each experimental set) were made with paired ureter sets derived from the same embryonic litters. Mouse experiments were approved by the University of Manchester ethics committee and the UK Home Office (licence PAFFCI44F). Organs were collected in RNA protect (Thermo Fisher Scientific, Runcorn, UK) and RNA extracted using the RNeasy Plus Mini Kit (Qiagen). Truseq stranded mRNA Assay
ENA-FIRST-PUBLIC
2019-08-07T17:03:22Z
ENA-LAST-UPDATE
2018-11-13T17:15:39Z
External Id
SAMEA5085232
INSDC center name
UNIVERSITY OF MANCHESTER
INSDC first public
2019-08-07T17:03:22Z
INSDC last update
2018-11-13T17:15:39Z
INSDC status
public
Submitter Id
E-MTAB-7395:Sample 5
broker name
ArrayExpress
common name
house mouse
developmental stage
embryonic day 15.0
organism part
ureter
sample name
E-MTAB-7395:Sample 5
scientific_name
Mus musculus
strain
CD1 mus strain