DMS5
Drosophila melanogaster
Protocols: Wandering third instar larvae were selected once outside the food. The eye imaginal discs were separated from the antenna and brain. The tissues were dissociated using trypsin and collagenase Following dissociation, the single cells suspension was washed in PBS and 0.01% BSA as suggested in the Drop-seq protocol Cell were lysed in the droplets generated in Drop-seq. RNA was captured on oligo dT beads Library construction was performed as written in the Drop-seq protocol (DOI:https://doi.org/10.1016/j.cell.2015.05.002). 1) RNA was reverse transcribed. 2) cDNA was PCR amplified. 3) tagmentation was done using Nextera XT kit.
ENA-FIRST-PUBLIC
2018-10-29T17:03:18Z
ENA-LAST-UPDATE
2018-09-18T09:49:46Z
External Id
SAMEA4921664
INSDC center name
University of Dhaka
INSDC first public
2018-10-29T17:03:18Z
INSDC last update
2018-09-18T09:49:46Z
INSDC status
public
Submitter Id
E-MTAB-7195:DMS5
broker name
ArrayExpress
common name
fruit fly
developmental stage
third instar larva stage
genotype
wild type genotype
organism part
eye disc
sample name
E-MTAB-7195:DMS5
scientific_name
Drosophila melanogaster
sex
mixed
strain
y1 v1