fw3.2(ys) 7 Days Post Anthesis Seed Replicate 1
Solanum lycopersicum
Lines nearly isogenic for fw3.2 in the cultivated background Solanum lycopersicum c.v. Yellow Stuffer were grown in the greenhouse in a completely randomized design.  fw3.2(ys) and fw3.2(wt) are NILs carrying cultivated and wild alleles for fw3.2 locus. Flowers were tagged a day before anthesis and self-pollinated at anthesis. Fruits at stage five, seven, and ten days post anthesis (dpa) were harvested. Pericarp and seed tissues were separately isolated. Four replicates were collected for each sample. RNA was extracted using Trizol and Stand-specific libraries were prepared from total RNA and sequences of 51 bp were generated on an Illumina HiSeq2000.  For details contact Dr. Esther van der Knaap (vanderknaap.1@osu.edu).
cultivar
Yellow Stuffer
tissue
7 Days Post Anthesis Seed