Sample 31
Homo sapiens
Protocols: Samples including lesional and non-lesional skin samples, were taken at each participant visit. Skin biopsies (6mm punch biopsies) were taken from lesional (edge of a plaque) and adjacent (minimum distance of 2cm) non-lesional skin from photoprotected sites on the lower back or upper buttock at each visit. Repeat biopsies were taken at a minimum distance of 2cm and biopsy sites were recorded. The dose of etanercept for all included participants was: Etanercept subcutaneous administration 25mg twice weekly (administered either as 25mg twice weekly or 50mg once weekly). mRNA was extracted from skin biopsies using Qiagen Rneasy mini kits Libraries were prepared with the TruSeq Stranded Total RNA Sample Preparation Kits. The first step involved removal of ribosomal RNA (rRNA), conducted using biotinylated Ribo-Zero rRNA removal beads. For RNA extracted from skin, the Ribo-Zero Gold kit was used to deplete samples of cytoplasmic and mitochondrial rRNA.
ENA-FIRST-PUBLIC
2018-09-21T17:02:08Z
ENA-LAST-UPDATE
2018-09-11T14:45:38Z
External Id
SAMEA4893754
INSDC center name
QMUL
INSDC first public
2018-09-21T17:02:08Z
INSDC last update
2018-09-11T14:45:38Z
INSDC status
public
Submitter Id
E-MTAB-6556:Sample 31
age
44
broker name
ArrayExpress
clinical history
non-smoker
clinical information
psoriasis area severity index 11.2
common name
human
disease
psoriasis
individual
AFOY01
organism part
skin
sample name
E-MTAB-6556:Sample 31
sampling site
lesion
scientific_name
Homo sapiens
sex
male
treatment
etanercept; 50 milligrams