PUF2 RNAi -tet
Trypanosoma brucei
Protocols: Cells were centrifuged out of the medium in vitro culture of bloodstream forms with standard HMI medium V5-PUF2 was immunoprecipitated and RNA was purified from the flow-through (FT) and bound eluate fractions myc-tagged UPF1 was purified and RNA was prepared from the bound eluate and flow-thorough (FT) fractions RNA was made using Trifast reagent (Peqlab) according to the manufacturer's instructions illumina kit
ENA-FIRST-PUBLIC
2018-05-21T17:01:59Z
ENA-LAST-UPDATE
2018-05-11T16:23:31Z
External Id
SAMEA4659726
INSDC center name
ZMBH
INSDC first public
2018-05-21T17:01:59Z
INSDC last update
2018-05-11T16:23:31Z
INSDC status
public
Submitter Id
E-MTAB-5617:PUF2 RNAi -tet
broker name
ArrayExpress
developmental stage
bloodstream form
genotype
inducible PUF2 RNAi
sample name
E-MTAB-5617:PUF2 RNAi -tet
scientific_name
Trypanosoma brucei
strain
Lister 427