Sample 12
Homo sapiens
Protocols: U2OS cells were grown in DMEM with 10% FCS and pen/strep and harvested in TriZol. U2OS cells were grown in DMEM with 10% FCS and pen/strep. Cells were treated with DOX to knockdown EDC4 or IKKb. Cell were irradiated 20 Gy then treated with Actinomycin D (ActD) was added two hours prior to harvest. Harvest in TriZol according to manufacturer's protocol Stranded mRNA sequencing libraries were prepared with 500ng total RNA according to manufacturer's protocol (Illumina).
ENA-FIRST-PUBLIC
2018-10-29T17:02:09Z
ENA-LAST-UPDATE
2018-09-26T14:38:46Z
External Id
SAMEA4939528
INSDC center name
Max Delbrueck Center for Molecular Medicine
INSDC first public
2018-10-29T17:02:09Z
INSDC last update
2018-09-26T14:38:46Z
INSDC status
public
Submitter Id
E-MTAB-7199:Sample 12
broker name
ArrayExpress
cell line
U2OS
cell type
epithelial cell
common name
human
compound
actinomycin D
disease
osteosarcoma
genotype
DOX inducible pTRIPZ shRNA_IKKbeta
irradiate
ionizing radiation (20 Gy)
organism part
bone tissue
sample name
E-MTAB-7199:Sample 12
scientific_name
Homo sapiens