Mg_transcriptome
Melocactus glaucescens
Plants germinated in vitro for 380 days had their apical stem segments removed and were sectioned transversely, generating explants of 34 mm in height . One explant was stocked in liquid nitrogen immediately after excision to be used as the control for comparative transcriptomics. A second explant from the same individual was punctured three times in the areola region with 0.18  8 mm needles to initiate shoot organogenesis, and was placed in a vertical position inside glass tubes containing 15 mL of MS full-strength medium supplemented with 17.76 M benzyladenine  and 1.34 M naphthalene acetic acid. The tubes were sealed with rigid polypropylene lids. Cultures were maintained at 25  3C under two fluorescent lamps with photosynthetically active radiation levels of 60 mol m-2 s-1 and a 16/8-h light/dark photoperiod. After 30 days of SO induction, five explants exhibiting shoot formation  were considered to have been induced and were therefore used for further analysis.
isolate
Cactaceae
cultivar
wild
ecotype
wild
age
1 year
dev_stage
in vitro organogenesis
geo_loc_name
Brazil: Bahia, Morro do Chapeu city
tissue
stem
biomaterial_provider
Laboratory of Tissue Culture - UFV
cell_line
not applicable
cell_type
not applicable
collected_by
Gabriela Torres da Silva and Andrea Dias Koehler
collection_date
not applicable
culture_collection
not applicable
disease
not applicable
disease_stage
not applicable
genotype
not applicable
growth_protocol
The seeds were disinfected with 96% ethanol for 1 min, NaOCl commercial bleach (2.5% ) at 2% for 10 min, and subsequently washed thrice in sterile water under aseptic conditions. Seeds were germinated in 500-mL glass flasks with rigid polypropylene lids , containing 50 mL of MS culture medium at quarter-strength concentration supplemented with 15 g L-1 sucrose and solidified with 7 g L-1 agar . Cultures were maintained at 25 3C under two fluorescent lamps with photosynthetically active radiation levels of 60 mol m-2 s-1 and a 16/8-h light/dark photoperiod.
height_or_length
Explants of stem with 3-4mm of height.
isolation_source
The samples are from in vitro seed germination of seed collected in the natural population located in Morro do Chapeu city, Bahia state, Brazil
lat_lon
11.3960507 S 41.2796251 W
phenotype
not applicable
population
Natural population
sample_type
stem
sex
not applicable
specimen_voucher
not applicable
temp
25C
treatment
Control was considered as stem samples before shoot organigenesisTreated was considered as stem samples after 30d of shoot organogenesis
BioSampleModel
Plant