NLS-GSyellow_1
Arabidopsis thaliana
Protocols: Cross-linking of Arabidopsis cell culture material was carried out as described (doi: 10.1104/pp.113.229617 Verkest et al., 2014). Cell suspension cultures were sub-cultured every 7 days by transfering 7 mL culture in 43 mL MSMO medium (containing per liter 4.43 g MSMO (minimal organics Sigma #M6899), 30g Sucrose, H2O, 50 μl kinetin (1 mg/ml stock solution) and 500 μl NAA (1mg/ml stock solution) and a pH adjusted to 5.7 with 1M KOH. Flasks were grown in the dark in an incubator at 25°C shaking at 130 rpm. ChIP-seq on PPD2-GSyellow was performed using 5 uL ab290 anti-GFP antibody (Abcam), with minor adaptations of a published protocol (Morohashi et al., 2012) and 1 g fixed material was used per ChIP. All subsequent steps were carried out as described by Morohashi et al. 2012. The PPD2-HBH TChAP-seq was performed using an Arabidopsis cell suspension culture overexpressing a HBH tagged PPD2 as described (doi: 10.1105/tpc.15.00006 Gonzalez et al., 2015) ChIP-seq libraries were prepared by GATC Biotech
ENA first public
2018-08-07
ENA last update
2018-02-16
External Id
SAMEA104598813
INSDC center alias
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
INSDC center name
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
INSDC first public
2018-08-07T17:03:06Z
INSDC last update
2018-02-16T14:52:50Z
INSDC status
public
Submitter Id
E-MTAB-6513:NLS-GSyellow_1
broker name
ArrayExpress
cell line
PSB-D
common name
thale cress
genotype
p35S:NLS-GSyellow
organism part
stem
sample name
E-MTAB-6513:NLS-GSyellow_1
ecotype
Landsberg erecta