Arab_S044-01-T06
Arabidopsis thaliana
spl7-1_Root_-Cu
isolate
spl7-1
ecotype
Col-0
age
7-day-old
dev_stage
seedling
geo_loc_name
China: Kunming,Yunnan
tissue
Root
biomaterial_provider
Plant mineral nutrition group, Xishuangbanna Tropical Botanical Garden, CAS
cell_line
not applicable
cell_type
not applicable
collected_by
Yuerong Cai
collection_date
2019-04
culture_collection
not applicable
disease
not applicable
disease_stage
not applicable
genotype
spl7-1
growth_protocol
Seeds were surface-sterilized with 20% commercial bleach for 15 min and then washed three times with double-distilled water. After plated on 1/2 MS media, seeds were vernalized for 2 d at 9°C before germination in greenhouse. Cu-sufficient (+Cu) medium is/2 MS media with 1% sucrose, 0.7% agar and 1μM CuSO4 at pH 5.8, and Cu-deficient (-Cu) medium is the same without CuSO4. Plates were placed in a culture room at 22°C under a 16 h light/8 h dark photoperiod. Seedlings grown on medium for 7 days were used for root harvest..
height_or_length
not collected
isolation_source
Not collected
lat_lon
Not collected
phenotype
not collected
population
not applicable
sample_type
Whole root
sex
hermaphrodite
specimen_voucher
not applicable
temp
Room temperature
treatment
-Cu
BioSampleModel
Plant