Col-0_1
Arabidopsis thaliana
Protocols: Seedlings were grown on half-strength (0.5×) Murashige and Skoog (MS) medium under the growth chamber conditions 16 h day/8 h night with white light and 20°C. 10-day old Arabidopsis seedlings at the developmental stage 1.02 (Boyes et al., 2001) were dissected with laboratory micro scissors to remove roots and cotyledons and obtain shoot apices. The Arabidopsis seeds were sterilized in 5% (v/v) bleach containing 0.05% (v/v) Tween 20 for 10 min, washed in water, sown on half-strength Murashige Skoog (MS) medium and stratified at 4°C for 48 h. Subsequently seedlings were grown under the growth chamber conditions 16 h day/8 h night with white light and 20°C. The poly-A containing mRNA molecules were selected and converted to cDNA using the reagents provided in the Illumina TruSeq Stranded mRNA Sample Preparation Kits. The dissected shoot apices were frozen in liquid nitrogen and homogenized. RNA was isolated with RNeasy Plant Kit (Qiagen) with on-column DNase digestion. The manufacturer's protocol was modified by including two additional washes of RNeasy spin columns with the RPE buffer. The library of cDNA template molecules of known strand origin was obtained using the reagents provided in the Illumina TruSeq Stranded mRNA Sample Preparation Kits.
ENA-FIRST-PUBLIC
2018-05-14T17:05:50Z
ENA-LAST-UPDATE
2018-05-11T15:55:43Z
External Id
SAMEA4659713
INSDC center name
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
INSDC first public
2018-05-14T17:05:50Z
INSDC last update
2018-05-11T15:55:43Z
INSDC status
public
Submitter Id
E-MTAB-6780:Col-0_1
age
10
broker name
ArrayExpress
common name
thale cress
developmental stage
Stage 1.02, two rosette leaves >1 mm in length
ecotype
Col-0
genotype
wild type genotype
organism part
shoot apex
sample name
E-MTAB-6780:Col-0_1
scientific_name
Arabidopsis thaliana