M-wt-Nickase-794-4
Homo sapiens
Protocols: HEK 293 cells were seeded and transfected with the indicated constructs for indicated time points. Genomic DNA was isolated using the DNeasy Blood & Tissue Kit (QIAGEN) according to the manufacturers instructions. Repaired plasmids and genomic junctions were PCR-amplified using specific primers with different 6bp 5 prime tags. For genomic amplicons of figure 7, the Nextera XT Index Kit (Illumina) was used. Amplicons were pooled and sequenced on the Illumina MiSeq platform.
ENA-FIRST-PUBLIC
2020-09-25T04:04:46Z
ENA-LAST-UPDATE
2019-09-27T11:05:52Z
External Id
SAMEA5977519
INSDC center name
1 Department of Internal Medicine III with Haematology, Medical Oncology, Haemostaseology, Infectiology and Rheumatology, Oncologic Center, Salzburg Cancer Research Institute Laboratory for Immunological and Molecular Cancer Research (SCRI LIMCR), Paracelsus Medical University, Salzburg, Austria, Cancer Cluster Salzburg, Austria 2 Department of Biosciences, Paris Lodron University of Salzburg, Austria
INSDC first public
2020-09-25T04:04:46Z
INSDC last update
2019-09-27T11:05:52Z
INSDC status
public
Submitter Id
E-MTAB-8382:M-wt-Nickase-794-4
broker name
ArrayExpress
cell line
HEK 293
cell type
epithelial cell
common name
human
developmental stage
embyo
genotype
wild type genotype
organism part
kidney
sample name
E-MTAB-8382:M-wt-Nickase-794-4
scientific_name
Homo sapiens
transfection
4nCas9 constructs + ssDNA oligo RG794