Auxin_M_1
Homo sapiens
source_name
HeLa S3 cells with a homozygous knock-in of the AID tag to the WAPL gene
cell type
HeLa S3
genotype/variation
homozygous knock-in of the AID tag to the WAPL gene
synchronization
Cells were arrested in G1/S with 2mM thymidine for 16 hours, released into fresh media for 3 hours, followed by 100nM nocodazole.
labeling
Nascent transcripts were labeled by incubation with 0.25mM EU for 12 hours during nocodazole arrest.