Phylogenomics demonstrate that breviate flagellates are related to opisthokonts: implications for the origin of genes involved in multicellularity
unclassified eukaryotes
Monoeukaryotic culture with accompanying bacteria. Total RNA was isolated from cells harvested by centrifugation using Tri-Zol reagent (Sigma-Aldrich, St Louis, Missouri, U.S.A.) using standard manufacturer's protocols. Poly(A)  RNA was isolated through two rounds of Poly(A)  selection and a paired-end cDNA library with a nominal insert size of ~477bp was constructed by Macrogen (Seoul, South Korea), following the ILLUMINA HISEQ 2000 RNA-seq library construction protocol (ILLUMINA, San Diego, California, U.S.A.). The cDNA library was then sequenced using a * of a multiplexed ILLUMINA HISEQ 2000 lane.