Bacteria from Nephrops norvegicus gut
Samples were collected from Pagasitikos Gulf (Greece) during September (sample Se3) and December (sample D1) of 2007. The  DNA used for the clone libraries was also analyzed with 454 pyrosequencing with the bacterial primers 27f and 519r (Muyzer et al. 1995) in order to amplify the V3 region of the 16S rRNA gene. Amplicon 454 pyrosequencing was performed with the bacterial primers 27F and 519R targeting the V1-V3 region of the 16S rRNA gene