S-7708_AS-160862_170306_EE_10a_3_1
Mus musculus
Protocols: Sorted murine primary BM cells from crushed hindlegs and spines (C57BL/6J, 8 weeks old, female) lineage depleted, StemCell Technologies) for Lin-,Sca-1+,c-Kit+ markers (40,000 cells). After 5 days, cells were sorted for GFP+ marker. Quiagen miRNeasy Micro Kit 1. cDNA: SMARTer Ultra Low Input RNA for Illumina Sequencing - HV (Clontech Laboratories, Inc.) 2. LibPrep: NEBNEXT ChIP-Seq Library Prep Master Mix Set for Illumina (Cat no. E6240) with modifications: a. Size exclusion omitted b. PCR with 10µl adapter-ligated ds cDNA and NEBNext High-Fidelity 2x PCR Master Mix (Cat no. M0541)
ENA first public
2018-06-01
ENA last update
2018-02-14
External Id
SAMEA104590709
INSDC center alias
(1) Department of Translational Oncology, National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ) (2) Faculty of Biosciences, University of Heidelberg, Germany
INSDC center name
(1) Department of Translational Oncology, National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ) (2) Faculty of Biosciences, University of Heidelberg, Germany
INSDC first public
2018-06-01T17:03:04Z
INSDC last update
2018-02-14T13:18:16Z
INSDC status
public
Submitter Id
E-MTAB-6496:S-7708_AS-160862_170306_EE_10a_3_1
age
8
broker name
ArrayExpress
cell type
bone marrow cell
common name
house mouse
genotype
miR-10a overexpression
organism part
mixed hindlimb and spine
phenotype
miR-10a overexpression; Lin-,Sca-1+,c-Kit+
sample name
E-MTAB-6496:S-7708_AS-160862_170306_EE_10a_3_1
sex
female
strain
C57BL/6J