Sample 9
Homo sapiens
Protocols: A431 cells with different treatment (TCF4 siRNAs, and processing times) were harvested for RNA-seq. The TCF4 siRNA oligo (Sense: 5'- CUCAUCGUCUCCUAAUUAUTT -3', Antisense: 5'- AUGAUUCCAUGCAUGUCCCTT -3') is used to transient transfected into A431 cells, while the siRNA oligo (Sense: 5'- UUCUCCGAACGUGUCACGUTT -3', Antisense: 5'- ACGUGACACGUUCGGAGAATT -3') is used as blank group by lipo2000 (Thermo Fisher, USA). And non-treated A431 cells are used as negative control. Total RNA was isolated from the samples with RNeasy Mini kit (QIAGEN, Gemany) in accordance to the manufacturers protocol. RNA was quantified using the NanoPhotometer spectrophotometer (Implen Inc., CA, USA). We then measured RNA concentration using Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA), and assessed RNA integrity using the RNA Nano 6000 Assay Kit in Bioanalyzer 2100 (Agilent Technologies, CA, USA). All the samples had a high RNA Integrity Number (RIN) score (>9.4). A total of 1ug of RNA per sample was submitted to the Genefund Co. Ltd (Shanghai, China) for library generation and sequencing. The sequencing library is generated by the kit of the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®(#E7760,New England Biolabs)
ENA-FIRST-PUBLIC
2019-07-06T04:02:51Z
ENA-LAST-UPDATE
2018-12-04T14:37:36Z
External Id
SAMEA5147778
INSDC center name
Department of Dermatology, The First Affiliated Hospital of the Army Medical University
INSDC first public
2019-07-06T04:02:51Z
INSDC last update
2018-12-04T14:37:36Z
INSDC status
public
Submitter Id
E-MTAB-7372:Sample 9
broker name
ArrayExpress
cell line
A431
cell type
epithelial cell
common name
human
disease
squamous cell carcinoma
genotype
TCF4 knockdown by siRNA
organism part
skin
sample name
E-MTAB-7372:Sample 9
scientific_name
Homo sapiens