Detection of Low-Frequency HIV-1 Drug Resistance Mutations by Ultra-Deep Sequencing in Na*ve HIV-1 Infected Patients: an Application in Clinical Routine
Human immunodeficiency virus 1
We aimed to evaluate the use of ultra-deep pyrosequencing (UDPS) for genotypic resistance testing in clinical routine, at the University Hospital of Bordeaux, France. We performed UDPS on reverse-transcriptase (RT) from 47 HIV-1 patients, naive of antiretroviral treatment and for whom a genotypic resistance testing was requested for clinical management.