Sample 9
Homo sapiens
Protocols: Separate stromal and tumour fractions in 25cm2 flasks were washed with PBS, incubated in accutase for 5-10 mins, counted and resuspended at 750,000 cells/ml in OCMI without cholera toxin. Fluidigm Protocol P/N 100-7168 (Vers. I1) Using C1 to generate single-cell cDNA libraries for mRNA sequencing Illumina Nextera XT DNA library preparation guide P/N 15031942 (Rev. c) modified according to Fluidigm Protocol P/N 100-7168 (Vers. I1)
ENA-FIRST-PUBLIC
2019-04-30T04:02:44Z
ENA-LAST-UPDATE
2018-10-04T10:41:51Z
External Id
SAMEA4952357
INSDC center name
The University of Manchester
INSDC first public
2019-04-30T04:02:44Z
INSDC last update
2018-10-04T10:41:51Z
INSDC status
public
Submitter Id
E-MTAB-7249:Sample 9
age
81
broker name
ArrayExpress
cell type
stromal cell
common name
human
disease
ovarian serous adenocarcinoma
disease staging
stage 3C
individual
OCM.38
organism part
ascitic fluid
sample name
E-MTAB-7249:Sample 9
scientific_name
Homo sapiens
sex
female
tumor grading
grade 3