BIOPSY_7367
Homo sapiens
Protocols: Tissue for ChIP-seq analyses was from patients who were treated at the Netherlands Cancer Institute.Three biopsies (14-gauge, 15 mm thick) were taken from each tumour, two of which where frozen with liquid nitrogen and one was formalin-fixed for direct clinical assessment. Tumour cell percentage was determined using Hematoxylin and Eosin (H&E), and tissue was only used for ChIP-seq analysis when tumour cell percentage was higher then 50%. All samples stained positive for Estrogen Receptor. Samples were processed and chromatin was extracted as described (Schmidt et al., Methods 48:3 pp240-248 2009) but with a number of modifications. During the immunoprecipitation, 100mg/ml glycogen (Invitrogen) or 20mg/ml recombinant histone 2B (M2505S; New England Biolabs) and 1mg/ml human mRNA (Invitrogen) was added as carriers for the ChIP. During the entire procedure, non-sticky eppendorf tubes were used (13-698-794; Fisher Scientific).
ENA-FIRST-PUBLIC
2013-04-01T17:02:48Z
ENA-LAST-UPDATE
2018-03-08T16:18:13Z
External Id
SAMEA1713767
INSDC center name
Cancer Research UK Cambridge Institute
INSDC first public
2013-04-01T17:02:48Z
INSDC last update
2018-03-08T16:18:13Z
INSDC status
public
Submitter Id
E-MTAB-1534:BIOPSY_7367
broker name
ArrayExpress
cell type
epithelial
common name
human
disease state
breast cancer
organism part
breast
sample name
E-MTAB-1534:BIOPSY_7367
scientific_name
Homo sapiens
sex
female