Generic sample from Danio rerio
Danio rerio
1nl of 1mM 5-AzadCyD was injected into 1-cell stage embryos, which were then incubated in 100uM 5-AzadCyD until sphere stage and collected.  Embryos injected with water served as control. Total RNA was extracted using  Qiagen AllPrep DNA/RNA/Protein mini kit (Cat # 80004), ribosomal RNA was depleted using RiboMinus kit (A10837-08, Eukaryote Kit) followed by directional RNA library preparation according to Illumina's standard protocol. Detailed experimental procedures and bioinformatics analysis can be found in the supplemental method section of the paper.
strain
Tubingen
label
PE: paired end, SE: single end
development stage
sphere