control1
Homo sapiens
Protocols: Cell samples were collected 96 hours after transfection by trypsinization. TPC1 cells were cultured at 37째C in 5% CO2 in DMEM medium supplemented with 10% FBS Transient transfection of DEC1 or control siRNA was performed using Lipofectamine RNAi MAX at 30 nM concentration Total RNA was extracted from cell pellets using Maxwell RSC Simply RNA Cells kit (Promega) RNA-Seq libraries were prepared starting from 1 ug of total RNA, using TRUSEQ stranded RNA LT kit (Illumina)
ENA first public
2018-06-04
ENA last update
2018-01-18
External Id
SAMEA104483103
INSDC center alias
Laboratory of traslational research. AUSL-IRCCS Reggio Emilia
INSDC center name
Laboratory of traslational research. AUSL-IRCCS Reggio Emilia
INSDC first public
2018-06-04T17:01:52Z
INSDC last update
2018-01-18T11:12:46Z
INSDC status
public
Submitter Id
E-MTAB-6394:control1
broker name
ArrayExpress
cell line
TPC1
cell type
epithelial cell
common name
human
disease
papillary thyroid carcinoma
organism part
thyroid gland
sample name
E-MTAB-6394:control1