HET6
Mus musculus
Protocols: We derived twelve independent stable lines of mouse embryonic fibroblasts (MEF) from six wildtype and six CTCF-hemizygous mouse E13.5 embryos. Total RNA from each MEF line was purified using QIAzol Lysis Reagent (Qiagen) following manufacturer's instructions; DNase treatment and removal was performed using the TURBO DNA-freeTM Kit (Ambion, Life Technologies) according to the manufacturer's instructions. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina).
ENA first public
2018-06-01
ENA last update
2017-11-30
External Id
SAMEA104410767
INSDC center alias
Cancer Research UK Cambridge Institute.
INSDC center name
Cancer Research UK Cambridge Institute.
INSDC first public
2018-06-01T17:03:02Z
INSDC last update
2017-11-30T16:11:43Z
INSDC status
public
Submitter Id
E-MTAB-6259:HET6
broker name
ArrayExpress
cell type
mouse embryonic fibroblast cell
common name
house mouse
developmental stage
embryonic day 13.5
genotype
heterozygous Ctcf knockout
sample name
E-MTAB-6259:HET6
sex
male
strain
C57BL/6