WT_I_2
Saccharomyces cerevisiae
Protocols: S. cerevisiae cells were grown according to standard procedures at 25 °C in synthetic complete medium with bases and amino acids omitted as necessary for selection and with glucose (2%) as a carbon source. The pCM185 vector (CEN, TRP1) from Euroscarf was used as backbone to create the Doxycycline-regulated (Tet off) Rho expression construct (pCM185-Rho-NLS) (Mosrin-Huaman et al., 2009). Strains transformed by Rho expression vector were grown under repressing conditions (5 µg/mL of Doxycycline) at 25°C in YNB medium supplemented with SC-TRP (Synthetic Complete Supplement Mixture from MP Biomedicals) and glucose as carbon source (final concentration 2%). To allow the expression of Rho, the yeast cells were grown the same medium but without Doxycycline. Growth was performed during 16 hours incubation at 25°C. Total RNA extraction was performed using the acidic hot phenol/chloroform method Libraries preparations were made by I2BC high-throughput sequencing platform (Gif sur Yvette) using Illumina ScriptSeq protocol after RiboZero treatment.
ENA-FIRST-PUBLIC
2019-12-04T04:02:31Z
ENA-LAST-UPDATE
2018-12-18T14:33:59Z
External Id
SAMEA5187603
INSDC center name
CNRS
INSDC first public
2019-12-04T04:02:31Z
INSDC last update
2018-12-18T14:33:59Z
INSDC status
public
Submitter Id
E-MTAB-7538:WT_I_2
broker name
ArrayExpress
common name
baker's yeast
genotype
pCM185-Rho-NLS
growth condition
YNB medium supplemented with SC-TRP and 2% glucose
phenotype
doxycycline-mediated repression of Rho
sample name
E-MTAB-7538:WT_I_2
scientific_name
Saccharomyces cerevisiae
stimulus
+doxycycline
strain
BMA41