Sample 2
Homo sapiens
Protocols: RNA substrates were made by in vitro transcription using SP6 or T7 RNA polymerase and DNA templates ,and biotinylated at 3' end using a biotinylation Kit. Biotinylated RNAs were first bound to the streptavidin beads, and then incubated with Hela NE in the polyadenylation condition for 20 minutes, after biotin-streptavidine binding, washing, pull-down sample were heated in 1XSSC buffer for elution, as suggested by the manual of the Dynalbeads M280. The eluted samples were further subject to RNA purification for RNA-seq. Strand-specific RNA-seq libraries were prepared following the described protocol (Hunt, A. G, 2015) with the RNA-biotin based pull-down sample, and single-end squenceing (SE50) was carried out in Illumina Genome Analyzer II platform.
ENA first public
2017-06-01
ENA last update
2017-02-01
External Id
SAMEA72575668
INSDC center alias
Sun Yat-sen University, China
INSDC center name
Sun Yat-sen University, China
INSDC first public
2017-06-01T17:02:07Z
INSDC last update
2017-02-01T15:14:59Z
INSDC status
public
Submitter Id
E-MTAB-5383:Sample 2
broker name
ArrayExpress
common name
human
genotype
U to C mutation in conserved cis-element AAUAAA
polyA site RNA substrate
SV40 late pre-mRNA
sample name
E-MTAB-5383:Sample 2