b3-b3-1
Arabidopsis thaliana
age
7 days old
dev_stage
Seedlings
ecotype
Col-0
geo_loc_name
USA
organism
Arabidopsis thaliana
sample_name
SRA
tissue
Whole Seedling
growth_protocol
Seedlings were grown for seven days in continuous white light on Gamborg B5 media.
sample_title
b3-b3-1
genotype
nf-yb2 b3
Extracted molecule
polyA RNA
Description
The purpose of this RNA Sequencing project was to investigate the transcriptional regulatory relationship between NF-YC3/4/9, NF-YB2/B3 and CO-SAIL. Methods: Total RNA was isolated, and then poly-A purified. 100ng of starting RNA was used to generate RNASeq libraries using the NEXTflex Illumina qRNA-Seq Library Prep Kit, and sequenced on an Illumina HiSeq2500 machine. Results: NF-YC3/4/9, NF-YB2/B3 and CO-SAIL have both shared and independent regulatory targets. Overall design: mRNA profiles of 7-day old Wild Type (WT), nf-yc triple (Q3), nf-yb3/b3, and CO-SAIL seedlings grown in continuous white light were generated from two biological replicates through paired end deep sequencing on an Illumina HiSeq2500 platform.
BioSampleModel
Plant