mRNA from Saccharomyces cerevisiae BY4743 grown in YP
Saccharomyces cerevisiae BY4743
BY4743 cells were grown in YP medium, and incubated until log phase (one day) at 30C. Cell harvests were normalized via cell counting. Cells were washed in dH2O and RNA extraction was performed using the phenol-chloroform method. Total RNA was ethanol precipitated out of solution and resuspended in nuclease-free water, and underwent three DNase treatments using DNaseI for 1 hr at 37C to remove genomic DNA, then subsequently purified using the RNA Clean and Concentrator-25 kit (ZymoResearch). New England Biolabs rRNA-depleted (Yeast RiboZero) stranded library preparation and RNA sequencing using the Illumina NovaSeq6000 S2 PE100 were conducted by the Genome Quebec Sequencing Centre at McGill University.
strain
BY4743
isolation_source
Cell culture
collection_date
2019-06
geo_loc_name
Canada: Ottawa
sample_type
mRNA
BioSampleModel
Microbe, viral or environmental