OC_RA
fungus metagenome
October Cliffs invaded
ENA-FIRST-PUBLIC
2017-06-01T17:02:08Z
ENA-LAST-UPDATE
2017-03-09T21:23:01Z
External Id
SAMEA101058418
INSDC center name
UNIVERSITY OF WESTERN ONTARIO
INSDC first public
2017-06-01T17:02:08Z
INSDC last update
2017-03-09T21:23:01Z
INSDC status
public
Submitter Id
OC_RA
adapters
Illumina forward: ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT; illumina reverse: CGG TCT CGG CAT TCC TGC TGA ACC GCT CTT CCG ATC T
collection date
2014-10-23
environment (biome)
Temperate hardwood forest
environment (feature)
riparian zone; sugar maple stand
environment (material)
soil
geographic location (country and/or sea)
Canada
geographic location (depth)
0.1
geographic location (elevation)
240
geographic location (latitude)
43°22'45.79N
geographic location (longitude)
80°20'36.56W
investigation type
metagenome
multiplex identifiers
AMV4.5N_7F CCAAGGCC AMDG_14R TGGCGGCT
nucleic acid amplification
https://www.researchgate.net/profile/Masanori_Saito4/publication/249467201_A_new_primer_for_discrimination_of_arbuscular_mycorrhizal_fungi_with_polymerase_chain_reaction-denature_gradient_gel_electrophoresis/links/5535e3580cf218056e92ab46.pdf?origin=publication_detail
nucleic acid extraction
https://www.zymoresearch.com/dna/microbial-environmental-dna-isolation-1/soil-fecal-plant-dna/zr-soil-microbe-dna-microprep
pcr conditions
94°C for 1 min, 29 cycles of 94°C for 30 s, 55°C for 30 s, 72°C for 18 s. No final extension step was used.
pcr primers
https://www.researchgate.net/profile/Masanori_Saito4/publication/249467201_A_new_primer_for_discrimination_of_arbuscular_mycorrhizal_fungi_with_polymerase_chain_reaction-denature_gradient_gel_electrophoresis/links/5535e3580cf218056e92ab46.pdf?origin=publication_detail
project name
PRJEB19724
sample name
OC_RA
sample weight for DNA extraction
0.25
scientific_name
fungus metagenome
sequencing method
Illumina Miseq 2x300 bp
soil environmental package
soil
storage conditions (fresh/frozen/other)
frozen
target gene
nuclear ribosomal DNA gene, small subunit