Sample 6
Homo sapiens
Protocols: Clles were washed 2x in PBS and lysd directly on growth plates using Qiagen RNEassy lysis buffer Cells were maintained in RPMI1640 media supplemented with 10% FBS & penn/strep. Cells were passaged every 2-3 days and discarded after no more than 20 passages Cells in log phase growth were treated with 500nM Neratinib or DMSO vehicle control for 20 hrs, commencing less than 24 hrs after reseeding in fresh media Total RNA was isolated using the RNEasy Mini Kit (Qiagen) according to manufacturer's instructions and DNA was depleted with the RNase-Free DNase Set cDNA was synthesized with the TruSeq Stranded mRNA Library Prep Kit (Illumina)
ENA first public
2018-06-01
ENA last update
2018-02-09
External Id
SAMEA104584918
INSDC center alias
BAH-GU
INSDC center name
University of Glasgow
INSDC first public
2018-06-01T17:03:04Z
INSDC last update
2018-02-09T15:29:27Z
INSDC status
public
Submitter Id
E-MTAB-6486:Sample 6
broker name
ArrayExpress
cell line
NCI-H2009
cell type
epithelial cell
common name
human
disease
lung adenocarcinoma
metastatic site
lymph node
organism part
lung
sample name
E-MTAB-6486:Sample 6