E7.8.328
Homo sapiens
Protocols: Human embryos were obtained from two cohorts at the Huddinge Karolinska Hospital. The first cohort was from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5%O2 in IVF plates containing 700_l of CCMTM media (Vitrolife) covered with 300_l of OvoilTM (Vitrolife)). The second cohort was from frozen E2 embryos thawed (ThawKit� Cleave, VitroLife) and cultured in G-1� Plus media (VitroLife) until E3 and then transferred to CCM� media (VitroLife) from E3-E7. Embros were dissociated into single cells by the use of enzymatic (TrypLE) and mechanical separation, and then handpicked with fine glass capillaries by mouth pipetting. Single blastomemeres were directly dispensed into into individual wells of a 8-strip tube containing lysis buffer. Picelli et al, Full-length RNA-seq from single cells using Smart-seq2, Nature Protocols, 2014
ENA-FIRST-PUBLIC
2016-04-06T17:01:25Z
ENA-LAST-UPDATE
2018-03-09T05:55:27Z
External Id
SAMEA3582189
INSDC center name
Department of Cell and Molecular Biology, Karolinska Institutet
INSDC first public
2016-04-06T17:01:25Z
INSDC last update
2018-03-09T05:55:27Z
INSDC status
public
Submitter Id
E-MTAB-3929:E7.8.328
broker name
ArrayExpress
cell
1416
common name
human
developmental stage
embryonic day 7
individual
E7.8
organism part
embryo
sample name
E-MTAB-3929:E7.8.328
scientific_name
Homo sapiens
single cell well quality
OK
treatment
No