Sample_2
Arabidopsis thaliana
Total RNA extraction using a Phenol-chloroform-LiCl extraction; RNA library preparation with polyA selection and RNA-sequencing using Illumina HiSeq (pair-end, 2x150bp)
replicate
RM2_Rep2
ecotype
Ecotype Col-0
age
12 day old
dev_stage
seedlings
geo_loc_name
USA: Columbia
tissue
Root
growth_protocol
Seedlings were grown in vitro on 0,5 x MS (0,5 % MES, no sucrose) under a long-day photoperiod (16h of light), with a light intensity of 120-140 µE, and at a temperature of 22 °C. 5 day after seed sowing, the seedlings were inoculated with 10 µL of Pseudomonas simiae WCS417r at 2.106 CFU.mL-1 (in 10 mM MgSO4 solution) in physical contact with their roots, or mock-treated (10 mM MgSO4 solution). The plates were sealed with two layers of parafilm and transferred back to the plant growth chamber. Seven days post inoculation, the root and shoot tissues were harvested (at mid-day, 8h light) for total RNA extraction and genome-wide gene expression analysis by RNA-sequencing.
sample_type
tissue sample
treatment
Mock-treated
BioSampleModel
Plant