Sample 4
Homo sapiens
Protocols: Cells were washed 2x in PBS and lysed directlt on growth plates using Qiagen RNEasy lysis buffer Cells were maintained in RPMI1640 media supplemented with 10% FBS and penn/strep. Cells were passaged every 2-3 days and discarded after no more than 20 passages Log phase growth cells were treated overnight with 250nM Neratinib or DMSO vehicle control for 20hrs, starting less than 24hrs after seeding in fresh media Total RNA was isolated using the RNEasy Mini Kit (Qiagen) according to manufacturer's instructions and DNA was depleted with the RNase-Free DNase Set cDNA was synthesized with the TruSeq Stranded mRNA Library Prep Kit (Illumina)
ENA first public
2018-06-01
ENA last update
2018-02-09
External Id
SAMEA104584910
INSDC center alias
BAH-GU
INSDC center name
University of Glasgow
INSDC first public
2018-06-01T17:03:04Z
INSDC last update
2018-02-09T15:10:49Z
INSDC status
public
Submitter Id
E-MTAB-6485:Sample 4
broker name
ArrayExpress
cell line
NCI-H358
cell type
epithelial cell
common name
human
disease
non-small cell lung carcinoma
metastatic site
alveolus
organism part
lung
sample name
E-MTAB-6485:Sample 4