Sample 2
Homo sapiens
Protocols: 7 million cells for each of three biological replicates were collected off plates using trypsin and stored in TRIzol at -80 C until RNA extraction. Total RNA was extracted from TRIzol (Invitrogen) following manufacturer protocol. Any DNA contamination was removed from the extracted total RNA using the RNase Free DNase (Promega) followed by column clean-up with RNeasy Mini Kit (Qiagen). Depletion of rRNA and RNA-seq libraries were prepared from 1ug of total RNA using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio USA, Inc.) following standard protocols.
ENA-FIRST-PUBLIC
2019-04-30T04:02:48Z
ENA-LAST-UPDATE
2019-02-13T15:38:58Z
External Id
SAMEA5336116
INSDC center name
The Children's Hospital of Philadelphia
INSDC first public
2019-04-30T04:02:48Z
INSDC last update
2019-02-13T15:38:58Z
INSDC status
public
Submitter Id
E-MTAB-7676:Sample 2
broker name
ArrayExpress
cell line
EndoC-betaH1
common name
human
replicate
2
sample name
E-MTAB-7676:Sample 2
scientific_name
Homo sapiens