ZFC4mut_2
Mus musculus
Protocols: The cells used are mouse embryonic stem cells. For RNAseq ESC lines were grown for 7-10d to obtain a healthy, fast growing culture. Then 3x105 cells were seeded to 6wells and cells were grown for 48h before isolating RNA using the AllPrep DNA/RNA kit (Qiagen). RNA was prepared from three independent cell seedings to obtain triplicate samples. DNA was removed using the TURBO DNAfree kit (Ambion) and RNA integrity was assessed on a bioanalyzer. 4µg RNA were used and ERCC Spike-ins were added to prepare sequencing libraries using the ScriptSeq Complete Gold Kit (Illumina). ScriptSeq Index Primers (Illumina) were used to multiplex libraries.
ENA-FIRST-PUBLIC
2019-05-01T04:02:49Z
ENA-LAST-UPDATE
2018-10-29T12:36:12Z
External Id
SAMEA5053635
INSDC center name
Bioinformatics Researcher
INSDC first public
2019-05-01T04:02:49Z
INSDC last update
2018-10-29T12:36:12Z
INSDC status
public
Submitter Id
E-MTAB-7343:ZFC4mut_2
broker name
ArrayExpress
cell line
ES-E14TG2a
cell type
embryonic stem cell
common name
house mouse
developmental stage
embryo
disease
normal
genotype
Sall4 ZFC4mut
organism part
inner cell mass
sample name
E-MTAB-7343:ZFC4mut_2
scientific_name
Mus musculus