Sample 5
Homo sapiens
Protocols: Cells were washed 2x with PBS and lysed directly on growth plates using Qiagen RNEasy lysis buffer Cells were maintained in RPMI1640 media supplemented with 10% FBS and Penn/Strep. Cells were passaged every 2-3 days for no more than 20 passages. Cells in log phase growth were treated with Neratinib (500nM) or DMSO vehicle control for 20 hrs, starting less than 24 hrs after seeding in fresh media. Nucleic acids were isolated using Qiagen RNEast kits according to the manufacturer's instructions cDNA was synthesized with the TruSeq Stranded mRNA Library Prep Kit (Illumina)
ENA first public
2018-06-01
ENA last update
2018-02-09
External Id
SAMEA104584905
INSDC center alias
BAH-GU
INSDC center name
University of Glasgow
INSDC first public
2018-06-01T17:03:04Z
INSDC last update
2018-02-09T14:47:29Z
INSDC status
public
Submitter Id
E-MTAB-6483:Sample 5
broker name
ArrayExpress
cell line
A549
cell type
epithelial cell
common name
human
disease
lung adenocarcinoma
organism part
lung
sample name
E-MTAB-6483:Sample 5