2980.4656.772387.S33.W101102
Mus musculus
Protocols: Antibodies were administered to SCID mice in saline vehicle by multiple SC injections at a fixed dose of 10 mg/kg. Mice were sacrificed on day 10. TA muscle samples were removed and subjected to transcriptome profiling. Total RNA was isolated from the proximal part of gastrocnemius muscle using spin column purification RNAeasy (QIAGEN, Hilden, Germany). 300 ng of total RNA from the gastrocnemius muscle, proximal part was reversed transcribed to cDNA using Qscript cDNA super mix (Quanta Biosciences, Gaithersburg, Maryland, USA) and was diluted into a final volume of 100 µL. Primers were designed using the software Primer 3 (http://biotools.umassmed.edu/bioapps/primer3_www.cgi) and ordered from Sigma-Aldrich (Sigma-Aldrich, St Louis, Missouri, USA). cDNA was amplified in triplicate using MyiQ™single color real time PCR detection system (Bio-Rad Laboratories, Inc., Hercules, California, USA).
ENA first public
2017-06-01
ENA last update
2017-05-22
External Id
SAMEA104091534
INSDC center alias
Regeneron
INSDC center name
Regeneron
INSDC first public
2017-06-01T17:01:27Z
INSDC last update
2017-05-22T17:59:29Z
INSDC status
public
Submitter Id
E-MTAB-4943:2980.4656.772387.S33.W101102
age
9
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
organism part
tibialis anterior
sample name
E-MTAB-4943:2980.4656.772387.S33.W101102
sex
male
strain
SCID
time
10
treatment
anti-activinA