Testis H3K4me3 ChIP-Seq followed by bisulfite sequencing
Mus musculus
source_name
Testis
strain
C57BL/6J
age
Adult
genotype
wild-type
antibody
Anti-H3K4me3 (Abcam 8580)
sequencing technique
ChIP-Seq followed by bisulfite sequencing
protocols
H3K4me3 ChIP-Seq was performed from whole testis as described, however the library preparation protocol was modified to perform bisulfite sequencing. Immediately following sequencing adapter ligation, we performed bisulfite conversion using the Qiagen EpiTect Bisulfite Kit (Cat # 59104). Subsequent library amplification was performed using KAPA HiFi Uracil+ Kit, which is designed to tolerate uracil residues in bisulfite-treated DNA.