Sample 1
Homo sapiens
Protocols: Cells were harvested via trypsinization, followed by a series of wash steps. 100,000 cells of each were spun down at 550xg for 5min, 4C. Supernatant was removed; 50uL of PBS was added to wash pellet. Spin conditions remained the same. The cell pellet was resuspended in 50ul cold lysis buffer (1 mM Tris-HCl, pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630), spun down immediately at 550xg for 10min, 4C. The nuclei resuspended in the transposition reaction mix (2x TD Buffer (Illumina Cat #FC-121-1030, Nextera), 2.5ul Tn5 Transposes (Illumina Cat #FC-121-1030, Nextera) and Nuclease Free H2O) on ice. The transposition reaction was incubated at 37C for 45min. The transposed DNA was then purified using a Qiagen MinElute Kit with 10.5ul elution buffer. The transposed library was then PCR amplified using Nextera primers for 12 cycles. The PCR reaction was subsequently cleaned up using AMPureXP beads (Agencourt).
ENA-FIRST-PUBLIC
2019-04-30T04:02:46Z
ENA-LAST-UPDATE
2018-12-17T11:56:33Z
External Id
SAMEA5181578
INSDC center name
The Children's Hospital of Philadelphia
INSDC first public
2019-04-30T04:02:46Z
INSDC last update
2018-12-17T11:56:33Z
INSDC status
public
Submitter Id
E-MTAB-7540:Sample 1
broker name
ArrayExpress
cell line
EndoC-betaH1
cell type
beta cell
common name
human
developmental stage
late embryo
disease
normal
organism part
pancreas
replicate
1
sample name
E-MTAB-7540:Sample 1
scientific_name
Homo sapiens