Patient_2_PLC
Homo sapiens
Protocols: Cartilage was obtained under Ethics Committee approval with written informed consent from 8 patients with symptomatic OA at total knee replacement (n=8, age range 65-79 years, mean age 70.3). Cartilage from paired osteochondral samples were isolated from the intact PLC (posterior lateral condyle) and the damaged DMC (distal medial condyle) for RNA-seq analysis. Strand specific RNA-seq libraries were generated from 0.5-1 g RNA using the TruSeq Stranded mRNA assay (Illumina, Inc.) using the manufacturers Stranded mRNA Sample Preparation Kit and 101bp paired-end reads were generated with each sample yielding at least 39 million total reads. Total RNA was extracted from 200-400 mg of cartilage using TRIzol (Life-technologies) reagent and homogenisation (Braun Mikrodismembrator) following freezing in liquid nitrogen. The RNA was purified using RNeasy Qiagen clean-up columns (Qiagen) and for sequencing had a RIN score of >6 (2200 TapeStation, Agilent Technologies).
ENA first public
2016-07-06
ENA last update
2016-01-19
External Id
SAMEA3727408
INSDC center alias
UOM
INSDC center name
University of Manchester
INSDC first public
2016-07-06T17:01:46Z
INSDC last update
2016-01-19T12:06:04Z
INSDC status
public
Submitter Id
E-MTAB-4304:Patient_2_PLC
age
69
broker name
ArrayExpress
common name
human
disease
osteoarthritis of the knee
modified mankin score
9
organism part
cartilage
patient number
2
phenotype
Intact
sample name
E-MTAB-4304:Patient_2_PLC
sequencing date
03/05/2014
sex
male