RNAseq of Ostrinia scapulalis (ABB) L4 larvae on maize
Ostrinia scapulalis
Egg masses were obtained in our laboratory by rearing moths collected 2013 in the field, near Versailles (France). Controlled infestations were established in insectproof outdoor cages filled with maize. 24 egg masses were deposited per cage of maize. Developed L4 larvae were sampled and stored at -80 degree Celsius. After RNA extraction from each L4, the RNA was quantified and equimolar amounts of individual etracts were pooled to obtain three replicate samples of each 20 individuals of equal sex ratio. Tagged cDNA TruSeq libraries were subsequently sequenced on an Illumina HiSeq2000 at the MGX platform (Montpellier, France).
isolate
L4 larvae developped from egg masses which were obtained in our laboratory by rearing moths collected 2013 in the field, near Versailles (France).
isolation_source
sampled on maize
collection_date
2013
geo_loc_name
France:Versaille
tissue
whole L4 larvae
dev_stage
L4
lat_lon
48.805278 N 2.135000 E
sex
pooled male and female
BioSampleModel
Invertebrate