recq4a recq4b ~1000 CO
Arabidopsis thaliana
Protocols: Whole Arabidopsis thaliana inflorescences were collected and immerged in ice-cold 10 % sucrose and then frozen at −20 °C. Arabidopsis thaliana were grown under long day conditions (16 hr light) and 20 degrees Celcius. Pollen genomic DNA was extracted and purified following the protocol described in Choi et al, 2017. Choi K., Yelina N.E., Serra H., Henderson I.R. (2017) Quantification and Sequencing of Crossover Recombinant Molecules from Arabidopsis Pollen DNA. In: Tiemann-Boege I., Betancourt A. (eds) Haplotyping. Methods in Molecular Biology, vol 1551. Humana Press, New York, NY ~300 or ~1000 PCR amplified crossover molecules were pooled, concentrated by isopropanol precipitation and gel purified. ~2 μg of DNA in 100 μl of TE (10 mM Tris, 1mM EDTA, pH 8.0) buffer was then sonicated to a size range of 300-400 bp using a Bioruptor (Diagenode). Sonicated DNA was gel purified, end-repaired and used to generate libraries (Tru-seq DNA sample prep kit, Illumina).
ENA first public
2018-07-01
ENA last update
2017-12-21
External Id
SAMEA104446638
INSDC center alias
University of Cambridge
INSDC center name
University of Cambridge
INSDC first public
2018-07-01T17:02:02Z
INSDC last update
2017-12-21T10:04:32Z
INSDC status
public
Submitter Id
E-MTAB-6333:recq4a recq4b ~1000 CO
age
7
broker name
ArrayExpress
common name
thale cress
cultivar
Columbia/Landsberg F1 hybrid
developmental stage
whole plant flowering stage
genotype
recq4a, recq4b mutant
organism part
pollen
sample name
E-MTAB-6333:recq4a recq4b ~1000 CO