PIK70_exp_SS
Helicobacter pylori P12
PIK70(deltaHPP12_511::aphA). Exponential phase. strand-specific mRNA-seq.This strain was grown in Brucella both containing 10% FBS up to mid-exponential phase (O.D.600 =0.4). Then, cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit for Gram-negative bacteria (Epicentre). cDNA library was constructed using SureSelect - Strand specific RNA library kit (Agilent Technologies). The indexed cDNA libraries were pooled and sequenced in HiSeq2500 platform (Illumina).
sample_name
PIK70_exp_SS