Hela snoRD50a ASO-1
Homo sapiens
Protocols: Hela cells no treatment or, NC ASO, snoRD50a ASOs treated, the concentration is 50 nM, we used RNAi max for ASO transfection, and harvest cells 2 days after transfection. RNAs recovered by Fip1 antibodies were ligated onto an 3' adaptor, RT reactions were carried out for converting RNAs to cDNA, cDNAs were further circularized, digested, and PCR amplified. PCR product were sent for sequencing.
ENA first public
2017-06-01
ENA last update
2017-02-06
External Id
SAMEA79718668
INSDC center alias
Center for stem cell biology and tissue engineering, Sun Yat-Sen University
INSDC center name
Center for stem cell biology and tissue engineering, Sun Yat-Sen University
INSDC first public
2017-06-01T17:02:07Z
INSDC last update
2017-02-06T11:13:46Z
INSDC status
public
Submitter Id
E-MTAB-5384:Hela snoRD50a ASO-1
broker name
ArrayExpress
cell line
HeLa
common name
human
sample name
E-MTAB-5384:Hela snoRD50a ASO-1