Sample 4
Ascaridia galli
Protocols: Adult females of A. galli were collected from the small intestine of naturally infected laying hens before and on day three during a 7-day treatment period with FLBZ (Verminator®, Elanco Animal Health/Eli Lilly Danmark A/S) at 1.43 mg/kg body weight and day. Chickens were euthanized and live worms at both time points were collected from the small intestine. Three independent biological replicates were collected on both occassions. The worms were washed extensively in phosphate buffered saline (PBS) (22°C) and then immediately submerged in RNA-later (Sigma-Aldricht), until they were individually transferred into Cryo-vials (Sigma-Aldricht) and frozen at -80°C. Total RNA was extracted and purified from the anterior end of A. galli worms using the Qiagen RNeasy Kit. RNA purification was performed using RNase-Free DNase Set (Qiagen). The libraries were prepared from 1µg total RNA using the TruSeq stranded mRNA sample preparation kit (Cat RS-122-2101/2102, Illumina Inc.) including polyA selection. The library preparation was performed according to the manufactors protocol (#15031047 and #15031048, rev E.)
ENA-FIRST-PUBLIC
2017-06-01T17:01:26Z
ENA-LAST-UPDATE
2017-05-16T18:32:16Z
External Id
SAMEA104028008
INSDC center name
unspecified
INSDC first public
2017-06-01T17:01:26Z
INSDC last update
2017-05-16T18:32:16Z
INSDC status
public
Submitter Id
E-MTAB-5705:Sample 4
broker name
ArrayExpress
developmental stage
adult
sample name
E-MTAB-5705:Sample 4
scientific_name
Ascaridia galli
sex
female