Sham_day7_GFP_S1
Mus musculus
Protocols: Tissue from ventricles of male adult mice was enzymatically digested into a single cell suspension and viable GFP+ CD31- cells were isolated using FACS sorting. Briefly, hearts were minced and incubated in collagenase type II (Worthington, USA) at 37°C before to be filtered on 40μm strainers. Cells were then resuspended in red cell lysis buffer followed by dead cell removal and immunostaining for 15 minutes on ice with fluorophore-conjugated antibodies and followed by FACS sorting for mentioned markers. Immediately after cell sorting and counting, the cells were loaded on the 10x Chromium microfluidics chip (3-prime V2). Cells were loaded on the 10x Chromium system and single cell RNA seq libraries were generated following the manufacturer's instructions. 10x Chromium 3-prime V2 protocol 10x Chromium 3-prime V2 protocol
ENA-FIRST-PUBLIC
2019-03-28T17:03:52Z
ENA-LAST-UPDATE
2018-11-27T14:06:47Z
External Id
SAMEA5137562
INSDC center name
VICTOR CHANG CARDIAC RESEARCH INSTITUTE
INSDC first public
2019-03-28T17:03:52Z
INSDC last update
2018-11-27T14:06:47Z
INSDC status
public
Submitter Id
E-MTAB-7376:Sham_day7_GFP_S1
age
8
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
fraction
GFP positive
genotype
B6.129S4-Pdgfratm11(EGFP)Sor/J
organism part
cardiac ventricle
sample name
E-MTAB-7376:Sham_day7_GFP_S1
scientific_name
Mus musculus
sex
male
strain
C57BL/6J