Sample14
Mus musculus
Protocols: Mice were housed in laminar flow cages and permitted ad lib access to sterile food and water. Mice were exposed to aerosolized bacteria in a nose-only inhalation chamber (In-Tox products, Moriarty, NM) using a computer interface to control pressures and flows (Biaera Technologies, Frederick MD). To characterize the early course of each infection, 3-4 mice were euthanized after 4, 24 and 48hrs with intraperitoneal pentobarbital and exsanguinated by external cardiac puncture. Ten micrograms of total RNA isolated from lung tissue (equal amounts pooled from 3 individual animals) was sequentially treated with MICROBEnrich and MICROBExpress kits (Ambion) to enrich for bacterial mRNA through removal of mammalian mRNA/rRNA and bacterial rRNA, respectively. The resultant bacterial mRNA was fragmented using a RNA fragmentation kit (Ambion), which yielded fragments of 60-200 nucleotides in length. Libraries for Illumina sequencing were prepared as recommended by Illumina.
ENA-FIRST-PUBLIC
2013-03-29T17:01:40Z
ENA-LAST-UPDATE
2018-03-08T16:20:54Z
External Id
SAMEA1714533
INSDC center name
Institute for Systems Biology
INSDC first public
2013-03-29T17:01:40Z
INSDC last update
2018-03-08T16:20:54Z
INSDC status
public
Submitter Id
E-MTAB-1571:Sample14
age
8 week
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
organism part
lung
sample name
E-MTAB-1571:Sample14
scientific_name
Mus musculus
specimen with known storage state
fresh specimen
strain
BALB/c
time post F. tularensis SchuS4 infection
4 hour