Sample 234
Mus musculus
Protocols: The mESCs (129/CAST) with and without a CRISPR- induced deletion of the Sox2 super-enhancer on the CAST allele was constructed in the Bing Ren lab[Ref 1]. Single cells of the 129/CAST cross (wild type) and with the Sox2 enhancer deletion on the CAST allele was grown as described[Ref 2] and later FACS sorted with BD Influx cell sorter (BD Biosciences) individually into 384-well plates containing 2.3 ul of the single-cell RNA-seq lysis buffer. Plates were immediately frozen until further processed. Ref 1: Li, Y. et al. CRISPR reveals a distal super-enhancer required for Sox2 expression in mouse embryonic stem cells. PLoS ONE 9, e114485 (2014). Ref 2: Supplemental Methods_nov20 Selvaraj, S., R Dixon, J., Bansal, V. & Ren, B. Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing. Nat. Biotechnol. 31, 1111-1118 (2013). Smart-seq2, indexed by Nextera i5 and i7 indices. We used ERCC spike-in RNA (Thermo Fischer Scientific, cat no. 4456740).
ENA first public
2018-05-18
ENA last update
2018-01-16
External Id
SAMEA104470770
INSDC center alias
KIS
INSDC center name
Karolinska Institutet
INSDC first public
2018-05-18T17:02:43Z
INSDC last update
2018-01-16T16:28:49Z
INSDC status
public
Submitter Id
E-MTAB-6362:Sample 234
broker name
ArrayExpress
cell line
F123
cell type
embryonic stem cell
common name
house mouse
developmental stage
embryo
genotype
heterozygous deletion of sox2 superenhancer (CAST allele)
organism part
inner cell mass
sample name
E-MTAB-6362:Sample 234
sex
male
strain
129SvEv x CAST/EiJ