EVs_DNase_Rep.3
Mus musculus
DNA extracted from DNase treated sEVs (EVs smaller-in-size than apoptotic bodies) released by  mouse embryos obtained from B6C3F1/Crl female mice and cultured from days E1.5 to E4.5. Sequencing using Illumina's NextEra XT DNA kit and a v2.0 300 cycles High Output cartridge, using the paired-end method in an NextSeq 550 Illumina's sequencer.
strain
B6C3F1/Crl
isolate
Not applicable
breed
Not applicable
cultivar
Not applicable
ecotype
Not applicable
age
6 weeks
dev_stage
E1.5
sex
female
tissue
Embryos
replicate
biological replicate three
biomaterial_provider
Charles River Laboratories (Crl)
genotype
B6C3F1
growth_protocol
Embryo culture in O/N oxygenated G2-Plus culture medium (Vitrolife) from day E1.5 to E4.5
health_state
Good/Normal
isolation_source
Isolated from the oviduct by flushing with pre-warmed PBS
sample_type
Smaller embryo derived extracellular vesicles
store_cond
37°C, 5% CO2
treatment
Dnase treated
BioSampleModel
Model organism or animal