Homo sapiens
PBMC were isolated from one individual. mRNA was extracted using standard Trizol method. First strand of cDNA were generated using primers specific for BCRs C-segments. Universal adapter was introduced using reverse transcriptase template switching effect on 5 end of cDNA molecules. cDNA was amplified in two steps of PCR. To the final PCR product Illumina TruSeq adapters were ligated using convenient protocol.
isolate
s1
age
45
biomaterial_provider
pp
sex
male
tissue
blood
BioSampleModel
Human