T3/External/Peel/C/Rep05
Pyrus communis
Peel tissue from fruit harvested from external canopy of trees, IAD Class C (1.80-1.89 IAD). Pooled tissue was ground to a fine powder with a SPEX 6875 Freezer/Mill in the presence of liquid nitrogen. RNA was extracted using a CTAB/Chloroform protocol modified specifically for tree fruit tissue.
tissue
Peel
treatment
after 8 Months CA Storage (− 0.5 °C, 2 kPa O2 and 0.8 kPa CO2)
temp
0.5 degrees Celcius
cultivar
d'Anjou'
age
not applicable
dev_stage
horticulturally mature fruit
geo_loc_name
USA: a commercial orchard approximately 3 miles west of Cashmere, Washington
biomaterial_provider
Washington State University Tree Fruit Research and Extension Center, 1100 N Western Ave, Wenatchee, WA 98801
collected_by
Washington State University Tree Fruit Research and Extension Center
collection_date
2014-09-14
isolation_source
A commercial orchard comprised of mature open vase trained 'd'Anjou' trees. 'd'Anjou' scion on 'Bartlett' seedling rootstock, 6m x 6m planting distance, 278 trees per ha with rows in an east to west oreientation planted in the 1970s was used.
lat_lon
47.5175 N 120.511486 W
sample_type
Biological Replicates are pooled tissue samples from 3 individual pear fruits
growth_protocol
commercial fruit production
library_strategy
RNA-seq
Library_preparation
Illumina TruSeq
library_source
TRANSCRIPTOMIC
library_selection
polyA
library_layout
Fragment
platform
Illumina
instrument_model
Illumina HiSeq 2500 in Rapid Mode
filetype
fastq
filename1
DAP-1-14_047C_techrep1.fastq.gz
filename2
DAP-1-14_047C_techrep2.fastq.gz
filename3
DAP-1-14_047C_techrep3.fastq.gz
filename4
DAP-1-14_047C_techrep4.fastq.gz
filename5
DAP-1-14_047C_techrep5.fastq.gz
BioSampleModel
Plant