Mus musculus embryonic stem cells ROSA26
Mus musculus
Mouse embryonic stem cells expressing an Enhanced Yellow Fluorescent Protein (EYFP) transgene from one ROSA26 locus and LacZ from the other ROSA26 locus, as well as tamoxifen-inducible CRE recombinase from untargeted viral integration, were maintained on a mouse embryonic fibroblast feeder layer by daily replacement of mES media [KO-DMEM (Invitrogen, San Diego, CA) containing 15% knock-out serum (Sigma, St. Louis, MO), 1X glutaMAX (Gibco, San Diego, CA), 1 X non-essential amino acids (Gibco), 55 μm 2-mercaptoethanol (Gibco), and 1000 U/mL mLIF (Millipore, Billerica, MA)]. These cells were a gift from A. McMahon (Harvard) (Hayashi and McMahon 2002; Mao et al. 2005).
breed
embryonic stem cells ROSA26 (A. McMahon, Harvard)