Abcc8-/-; 3244-MAM-8
Mus musculus
Protocols: Abcc8+/+; MIP-GFP and Abcc8-/-; MIP-GFP islets were collected from mice at age postnatal day 60. The islets from 3-7 mice of the same genotype were pooled for each sample. Islets were dispersed using Accumax and FACS purified using the MIP-GFP transgene and 7AAD viability dye to collect only live beta cells. Mice used in this experiment were null for the Abcc8 gene, which encodes a subunit of the beta cell ATP-sensitive potassium channel. Mice lacking this gene have persistent depolarization of the beta cell plasma membrane. Abcc8-/- mice were maintained on a C57BL/6 background and were bred to mice expressing the MIP-GFP transgene, which are maintained on a CD-1 background. Abcc8-/-; MIP-GFP mice and control Abcc8+/+; MIP-GFP mice were maintained on a mixed genetic background that was derived equally from C57BL/6 and CD-1 strains. Total RNA was extracted from FACS-purified MIP-GFP beta cells using the Maxwell simplyRNA kit (Promega). RNA was amplified using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), and cDNA libraries were constructed using the Low Input Library Prep Kit (Clontech).
ENA first public
2017-06-02
ENA last update
2016-10-18
External Id
SAMEA4516687
INSDC center alias
Vanderbilt University
INSDC center name
Vanderbilt University
INSDC first public
2017-06-02T17:02:09Z
INSDC last update
2016-10-18T09:58:21Z
INSDC status
public
Submitter Id
E-MTAB-4726:Abcc8-/-; 3244-MAM-8
age
60
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
Abcc8-/-
organism part
endocrine pancreas
sample name
E-MTAB-4726:Abcc8-/-; 3244-MAM-8
strain
Mixed C57BL/6J; CD-1