Generic sample from Danio rerio
Danio rerio
Maternal haploid embryos were generated according to standard procedure (Westerfield, 2000), using sperm from ÂgoldenÂ homozygous males  gol b1/b1 as a marker. DNA from sphere-stage maternal haploid, and sphere-stage control IVF diploids was extracted using phenol-chloroform and bisulfite converted using the EpiTect Bisulfite Kit (Qiagen, Valencia, CA 91355), followed by PCR amplification of 15 promoter regions. Genomic library was prepared according to Illumina's standard procedure and sequenced on Illumina MiSeq. Detailed experimental procedures and bioinformatics analysis can be found in the supplemental method section of the paper.
strain
Tubingen
label
PE: paired end, SE: single end
development stage
sphere