SM6_uc1
Homo sapiens
Protocols: The plasma was prepared from EDTA blood by centrifugation at 1,0006g for 15 minutes to separate the plasma and blood cells. Total RNA from plasma was extracted from 100 ml of the sample using the miRNeasy kit (Qiagen, Valencia, CA). The quality and quantity of the RNA were evaluated with Agilent 2100 Bioanalyzer (Santa Clara, CA) and NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE). In average, we obtained about 100 ng of RNA per ml of plasma. As a control we also obtained total RNA from Ambion (Life Technologies, Carlsbad, CA). NextGen sequencing libraries were prepared with small RNA sample preparation kits from Illumina (Illumina, San Diego, CA).
ENA-FIRST-PUBLIC
2013-03-29T17:01:40Z
ENA-LAST-UPDATE
2018-03-08T16:20:53Z
External Id
SAMEA1714517
INSDC center name
Institute for Systems Biology
INSDC first public
2013-03-29T17:01:40Z
INSDC last update
2018-03-08T16:20:53Z
INSDC status
public
Submitter Id
E-MTAB-1568:SM6_uc1
broker name
ArrayExpress
common name
human
disease state
ulcerative colitis
organism part
plasma
sample name
E-MTAB-1568:SM6_uc1
scientific_name
Homo sapiens