RHAL1_MetOH_La_3
Beijerinckiaceae bacterium
Protocols: Biomass was collected by centrifugation (10000 x g, 10 min, at room temperature). Pelleted biomass was flashfrozen and kept at -80 °C before being processed. Beijerinckiaceae bacterium RHAL1 was grown to exponential phase in minimal medium containing methanol as carbon source (1 % [v/v]) and lanthanum as growth supplement. Total RNA was extracted following a protocol modified after Wegner et al., 2013 (doi: 10.1016/j.margen.2012.12.001). Total RNA was enriched for mRNA by rRNA depletion using the MICROBExpress bacterial mRNA enrichment kit (Thermo Fisher Scientific, Schwerte, Germany). RNA-Seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs, Frankfurt, Germany).
ENA-FIRST-PUBLIC
2019-08-08T04:05:05Z
ENA-LAST-UPDATE
2019-07-24T13:21:48Z
External Id
SAMEA5814855
INSDC center name
Friedrich Schiller University Jena Institute of Biodiversity Aquatic Geomicrobiology
INSDC first public
2019-08-08T04:05:05Z
INSDC last update
2019-07-24T13:21:48Z
INSDC status
public
Submitter Id
E-MTAB-8184:RHAL1_MetOH_La_3
broker name
ArrayExpress
genotype
wild type genotype
growth condition
methanol 1 percent; lanthanum 1 micromolar
organism part
whole organism
sample name
E-MTAB-8184:RHAL1_MetOH_La_3
scientific_name
Beijerinckiaceae bacterium
strain
RH AL1