Sample 3
Arabidopsis thaliana
Protocols: Three biological repeats of the wild type (Col-0-A, Col-0-B and Col-0-C) and the mutant dcc1 (dcc1-A, dcc1-B and dcc1-C) after culture on shoot-induction medium for 16 days were used for RNA sequencing. Total RNAs were isolated from the calli of wild type Col-0 and the mutant dcc1 with TRI reagent (Sigma, catalog number T9424). The mRNA was enriched by Ribo-ZeroTM Magnetic Kit (Epicentre), and fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with random primers. Second-strand cDNA were synthesized by DNA polymerase I. Then the cDNA fragments were purified with QiaQuick PCR extraction kit, end repaired, poly(A) added, and ligated to Illumina sequencing adapters. The ligation products were size selected by agarose gel electrophoresis, and amplified by PCR to construct the RNA-seq llibraries.
ENA first public
2018-05-06
ENA last update
2016-11-14
External Id
SAMEA4545708
INSDC center alias
College of Life Sciences, Shandong Agricultural University
INSDC center name
College of Life Sciences, Shandong Agricultural University
INSDC first public
2018-05-06T17:04:22Z
INSDC last update
2016-11-14T14:15:31Z
INSDC status
public
Submitter Id
E-MTAB-5236:Sample 3
age
16
broker name
ArrayExpress
common name
thale cress
ecotype
Col-0
genotype
wild type genotype
growth condition
cultured on shoot-induction medium
organism part
plant callus
sample name
E-MTAB-5236:Sample 3