COSAIL-2
Arabidopsis thaliana
age
7 days old
dev_stage
Seedlings
ecotype
Col-0
geo_loc_name
USA
organism
Arabidopsis thaliana
sample_name
SRA
tissue
Whole Seedling
genotype
co-sail
sample_title
COSAIL-2
growth_protocol
Seedlings were grown for seven days in continuous white light on Gamborg B5 media.
Extracted molecule
polyA RNA
Description
The purpose of this RNA Sequencing project was to investigate the transcriptional regulatory relationship between NF-YC3/4/9, NF-YB2/B3 and CO-SAIL. Methods: Total RNA was isolated, and then poly-A purified. 100ng of starting RNA was used to generate RNASeq libraries using the NEXTflex Illumina qRNA-Seq Library Prep Kit, and sequenced on an Illumina HiSeq2500 machine. Results: NF-YC3/4/9, NF-YB2/B3 and CO-SAIL have both shared and independent regulatory targets. Overall design: mRNA profiles of 7-day old Wild Type (WT), nf-yc triple (Q3), nf-yb3/b3, and CO-SAIL seedlings grown in continuous white light were generated from two biological replicates through paired end deep sequencing on an Illumina HiSeq2500 platform.
BioSampleModel
Plant