Acyrthosiphon pisum head
Acyrthosiphon pisum
Acyrthosiphon pisum individuals (strain LSR1) were reared on broad bean Vicia fabae at low density (less than five individuals per plant) to prevent the production of winged morphs. Parthenogenesis was maintained under a 16h photoperiod and a temperature of 18°C. One hundred asexual adult females were frozen into liquid nitrogen for heads samples dissection. All samples (in duplicates for a total of 4 samples) were kept for subsequent RNA extractions performed using the SV Total RNA Isolation System (Promega) according to manufacturer's instructions. RNA quality was checked on Bioanalyzer (Agilent) and quantified on Nanodrop (Thermo Scientific).
replicate
biological replicate 2
strain
LSR1
dev_stage
adult
sex
female
tissue
head
biomaterial_provider
Julie Jacquiery (UMR 1349, France)
BioSampleModel
Model organism or animal