Sample 4
Mus musculus
Protocols: Hearts were harvested in icecold PBS with 0.5 mM EDTA and dissociated as described (Van Handel et al. 2012), using collagenase (10 mg/ml; Worthington) and dispase (10 mg/mL, Invitrogen). Cells were stained for 30 minutes, 4',6-diamidino-2-phenylindole (DAPI, 1:1000) was used to label dead cells. Tie2Cre lineage cells, negative for blood markers CD41, CD45 and Ter119, were sorted on an Influx™ cell sorter (BD Biosciences) and collected in RNA lysis buffer (Agilent) or TRIzol reagent (Ambion) for RNA extraction, or PBS/5% serum for ATAC-Seq (Buenrostro et al. 2013). Van Handel B, Montel-Hagen A, Sasidharan R, Nakano H, Ferrari R, Boogerd CJ, Schredelseker J, Wang Y, Hunter S, Org T, et al. 2012. Scl represses cardiomyogenesis in prospective hemogenic endothelium and endocardium. Cell 150: 590-605. Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. 2013. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods 10: 1213-8. We performed ATAC-seq according to Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. 2013. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods 10: 1213-8.
ENA first public
2017-06-02
ENA last update
2017-06-02
External Id
SAMEA104112370
INSDC center alias
University of Chicago
INSDC center name
University of Chicago
INSDC first public
2017-06-02T17:02:13Z
INSDC last update
2017-06-02T15:52:59Z
INSDC status
public
Submitter Id
E-MTAB-3972_2:Sample 4
batch
2014-11-05
broker name
ArrayExpress
cell type
endocardial cell
common name
house mouse
developmental stage
embryonic day 12.5
genotype
Tbx20 knockout
organism part
heart
replicate
2
sample name
E-MTAB-3972_2:Sample 4
strain
Black Swiss mouse strain