HET1_input
Mus musculus
Protocols: MEFs were fixed in DMEM containing 1% fresh formaldehyde and incubated at room temperature for 10 min, quenched with 250mM glycine for 10 min, and washed twice with ice cold PBS, before being flash-frozen at -80°C. Cross-linked cells were lysed and sonicated on a Bioruptor Plus (Diagenode) sonicator to fragment chromatin to an average length of 300bp. Immunoprecipitated DNA or 50 ng of input DNA was used for library preparation using the ThruPLEX DNA-Seq library preparation protocol (Rubicon Genomics, UK).
ENA first public
2018-06-01
ENA last update
2017-11-30
External Id
SAMEA104410858
INSDC center alias
Cancer Research UK Cambridge Institute.
INSDC center name
Cancer Research UK Cambridge Institute.
INSDC first public
2018-06-01T17:03:02Z
INSDC last update
2017-11-30T16:15:51Z
INSDC status
public
Submitter Id
E-MTAB-6261:HET1_input
broker name
ArrayExpress
cell type
mouse embryonic fibroblast cell
common name
house mouse
developmental stage
embryonic day 13.5
genotype
heterozygous Ctcf knockout
replicate
1
sample name
E-MTAB-6261:HET1_input
sex
female
strain
C57BL/6