Sample 6
Mus musculus
Protocols: Lung tissue was formalin fixed and paraffin embedded. Adjacent sections were stained for phospho-ERK expression. Upon orientation of tumours, p-ERK high and p-ERK low tumour regions were captured by Laser Capture Microdissection. Multiple isolates from multiple tumours were collected from each of 4 mice and pooled by p-ERK status (ie. 2 pools per mouse). P-ERK levels were determined by IHC and were binary as either very weak or very strong signal. Mice were euthanised humanely by CO2 inhalation followed by cervical dislocation. The chest cavity was opened surgically and mice were cardiac perfused with PBS, followed by formalin. Lungs and trachea were removed intact and gently inflated with formalin. Tissue was fixed overnight prior to dehydration and embedding in paraffin All mice were induced at 8 weeks of age and harvested 6 weeks after induction For tumour induction, mice aged 8 weeks were anaesthetised and inoculated with 10^7 Adeno-CMV-CRE particles by intra-nasal inhalation as described in Murphy, Junttila et al., Cancer Cell 2008. Tumours were allowed to develop over the ensuing 6 weeks without further intervention. Total tissue RNA was isolated using the RNEasy FFPE kit according to manufacturer's directions and ribosomal RNA was depleted with Ribo-Zero (Epicentre). Synthesis of cDNA was performed using the SMARTER Stranded random primed RNA-SEQ kit (Takara/Clontech), resulting in cDNA libraries flanked by Illumina indexing primers.
ENA first public
2018-06-01
ENA last update
2018-01-31
External Id
SAMEA104558237
INSDC center alias
BAH-GU
INSDC center name
University of Glasgow
INSDC first public
2018-06-01T17:03:03Z
INSDC last update
2018-01-31T17:17:58Z
INSDC status
public
Submitter Id
E-MTAB-6432:Sample 6
age
14
broker name
ArrayExpress
common name
house mouse
disease
adenocarcinoma
genotype
lsl-KRas^G12D;Rosa26-lsl-MYC
organism part
lung epithelium
phenotype
p-ERK High
sample name
E-MTAB-6432:Sample 6
sampling site
neoplasm
strain
FVBN x C57BL/6