WT4
Mus musculus
Protocols: We derived independent stable lines of mouse embryonic fibroblasts (MEF) from wildtype and CTCF-hemizygous mouse E13.5 embryos. For three biological replicates, cells were fixed in DMEM containing 2% fresh formaldehyde and incubated at room temperature for 10 min, quenched with 1M glycine for 5 min, and washed twice with ice cold PBS, before being flash-frozen at -80°C. Cross-linked cells were lysed, followed by chromatin HindIII digestion, biotinylataion, ligation, proteinase K treatment, DNA purification, sonication, end repair and biotin pull-down. Pulled-down DNA had adapters ligated and material was PCR amplified.
ENA first public
2018-06-01
ENA last update
2017-12-01
External Id
SAMEA104411345
INSDC center alias
Cancer Research UK Cambridge Institute.
INSDC center name
Cancer Research UK Cambridge Institute.
INSDC first public
2018-06-01T17:03:02Z
INSDC last update
2017-12-01T09:40:18Z
INSDC status
public
Submitter Id
E-MTAB-6262:WT4
broker name
ArrayExpress
cell type
mouse embryonic fibroblast cell
common name
house mouse
developmental stage
embryonic day 13.5
genotype
wild type genotype
replicate
4
sample name
E-MTAB-6262:WT4
sex
male
strain
C57BL/6