89107
Mus musculus
Protocols: 4 week old mice were sacrificed and DBA-positive renal tubules were isolated as previously described (Outeda, P.et al. Kidney international, 92(5), 1130-1144 (2017). & Dafinger, C. et al. Experimental & molecular medicine, 50(6), 75 (2018).). Total RNA was isolated from the DBA enriched fraction by using RNA using phenol-chloroform extraction. All the RNA samples were further treated with DNAse to eliminate genomic DNA contamination as per manufacture's instruction (Quick RNA kit, Zymoresearch). For RNAseq, libraries were prepared using TruSeq stranded mRNA kit (Illumina) according to the provider's protocol.
ENA-FIRST-PUBLIC
2019-03-31T17:01:55Z
ENA-LAST-UPDATE
2018-07-25T13:27:21Z
External Id
SAMEA4803575
INSDC center name
Uniklinik Koln, Klinik II fur Innere Medizin Nephrolab Cologne, CECAD
INSDC first public
2019-03-31T17:01:55Z
INSDC last update
2018-07-25T13:27:21Z
INSDC status
public
Submitter Id
E-MTAB-7077:89107
age
4
broker name
ArrayExpress
cell type
kidney cortex tubule cell
common name
house mouse
disease
Nephronophthisis
genotype
Aatf homozygous knockout
organism part
proximal tubule
sample name
E-MTAB-7077:89107
scientific_name
Mus musculus
sex
male
strain
C57BL/6