iCLIP-seq of Flag-Srrm4 in in-vitro differentiated neurons, replicate 1
Mus musculus
source_name
Cortical glutamatergic neurons differentiated in vitro from ES cells
cell line
Neurons derived from CGR8 ES cells
treatment
CGR8 ES cells were engineered to express Flag-tagged Srrm4 under the control of doxycyclin using the PiggyBac system, and single-cell subclones were derived. Cell were differentiated into cortical glutamatergic neurons as described previously (Hubbart et al., F1000 Res 2013). Neural precursor cells were dissociated and plated onto 15 cm plates coated with poly-D-Lysine and laminin (~20 million cells per plate). After 8 days Flag-Srrm4 expression was induced with 2 μg/ml doxycycline for 48 hours.
antibody
Flag monoclonal antibody M2 (Sigma-Aldrich)