L3.6pl_ATAC_C1
Homo sapiens
Protocols: ATAC was performed following the protocol of Buenrostro et al. Briefly, 50,000 L3.6pl cells were trypsinized and washed twice with cold PBS. Then cells were re-suspended in lysis buffer (10mM Tris-HCl pH 7.5, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated for 15 minutes at a rotating wheel at 4ºC followed by centrifugation and in re-suspended in 50 µl of transposition mix composed of 2.5µl of TDE1 (Nextera Tn5 Transposase), 25 µl TD (2x reaction buffer) in nuclease free water (Nextera DNA Library Prep Kit, FC-121-1030, Illumina). Then it was incubated at 37 degrees for 30 minutes. MinElute PCR Purification kit (Qiagen) was used for DNA extraction following the instructions of the manufacturer. ATAC libraries were made using the Nextera DNA Library Prep Kit according to Buenrostro et al. The libraries were amplified for 15 cycles in total of: 98ºC for 10 seconds, 63ºC for 30 seconds, and 72ºC for 60 seconds.
ENA-FIRST-PUBLIC
2018-12-18T17:02:52Z
ENA-LAST-UPDATE
2018-07-20T14:53:24Z
External Id
SAMEA4799825
INSDC center name
Section of Tumor Epigenetics, Department of General, Visceral and Pediatric Surgery, University Medical Center Gottingen, 37075 Gottingen, Germany
INSDC first public
2018-12-18T17:02:52Z
INSDC last update
2018-07-20T14:53:24Z
INSDC status
public
Submitter Id
E-MTAB-7035:L3.6pl_ATAC_C1
broker name
ArrayExpress
cell line
L3.6pl
cell type
epithelial cell
common name
human
disease
pancreatic adenosquamous carcinoma
metastatic site
celiac lymph node
organism part
pancreas
sample name
E-MTAB-7035:L3.6pl_ATAC_C1
scientific_name
Homo sapiens