Sample 3
Mus musculus
Protocols: Tail tendon fascicles were extracted from mice straight after euthanasia and cultured for 6 days in serum containing mediuam (10%) at either 3% oxygen and 29°C or 21% oxygen and 37°C. 16 to 20 fascicles were pooled and snap frozen before RNA extraction. Samples were pulverized in QIAzol lysis reagent (#79306, Qiagen) by cryogenic grinding (FreezerMill 6870, SPEXTMSamplePrep). Tissue lysates were mixed with 1-bromo-3-chloropropane (B9673, Sigma-Aldrich) at a ratio of 1:4 and the RNA containing aqueous/organic phase was separated using 5Prime PhaseLock Gel Heavy (#2302830, LabForce). RNA was extracted by using the RNeasy micro Kit (74004, Quiagen). The quantity and quality of the isolated RNA was determined with a Bioanalyzer 2100 (Agilent, Waldbronn, Germany). A total RNA amount of 100ng was used with the TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc, California, USA) for library preparation.
ENA-FIRST-PUBLIC
2019-04-30T04:02:50Z
ENA-LAST-UPDATE
2019-03-29T14:01:21Z
External Id
SAMEA5542000
INSDC center name
Institute for Biomechanics, ETH Zurich, Switzerland University Hospital Balgrist, University of Zurich, Switzerland
INSDC first public
2019-04-30T04:02:50Z
INSDC last update
2019-03-29T14:01:21Z
INSDC status
public
Submitter Id
E-MTAB-7832:Sample 3
age
87
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
3
organism part
tail
sample name
E-MTAB-7832:Sample 3
scientific_name
Mus musculus
sex
male
stimulus
21%O2, 37C
strain
C57BL/6