Sample 2
Mus musculus
Protocols: Mouse spleen isolated, physically disrupted with ground glass, filtered through 70uM mesh, erythrocytes lysed via red blood cell lysis buffer, filtered through 70uM mesh. Cell suspensions were evaluated by light microscopy for debris and viability, and were counted using a hemocytometer. Cell concentrations were 932 and 888 live cells / ul respectfully. Targeting approximately 2400 single cells per sample, single cells were captured, lysed, and RNA was reverse transcribed and barcoded using a 10x Genomics Chromium instrument and Chromium Single Cell 3' Reagent Kits v2 reagents. (10x Genomics, Pleasonton, CA). To construct Illumina compatible sequencing libraries the cDNA is fragmented, A-tail repaired and a double-sided bead cleanup is performed. Adapters are ligated to the cDNA fragments and the fragments are PCR amplified using unique sample index primers per manufacturer's recommendations. Libaries were quantified by qPCR using the KAPA Library Quant Kit (Illumina) from KAPA Biosystems (Roche, Pleasonton, CA).
ENA-FIRST-PUBLIC
2019-03-29T17:02:10Z
ENA-LAST-UPDATE
2019-03-19T11:04:27Z
External Id
SAMEA5522502
INSDC center name
UNIVERSITY OF NEBRASKA MEDICAL CENTER
INSDC first public
2019-03-29T17:02:10Z
INSDC last update
2019-03-19T11:04:27Z
INSDC status
public
Submitter Id
E-MTAB-7780:Sample 2
age
10
broker name
ArrayExpress
cell type
leukocyte
common name
house mouse
developmental stage
adult
environmental stress
social defeat stress
genotype
wild type genotype
individual
2
organism part
spleen
sample name
E-MTAB-7780:Sample 2
scientific_name
Mus musculus
sex
male
strain
C57BL6J