cytRNA-Aniso-RIII
Homo sapiens
Protocols: Cells were seeded 48 hours prior to stress treatment. Cells were subsequently treated with 5 ??g/ml Anisomycin for 3 hours and harvested in PBS. Total RNA was extracted using Trizol LS. Cells were fractionated and cytoplasmic RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions. 3'T-fill libraries were created as described in Wilkening S, et al. An efficient method for genome-wide polyadenylation site mapping and RNA quantification. Nucleic Acids Research. 2013;41(5):e65. doi:10.1093/nar/gks1249.
ENA-FIRST-PUBLIC
2016-07-13T17:01:22Z
ENA-LAST-UPDATE
2018-03-09T01:25:56Z
External Id
SAMEA3403321
INSDC center name
European Molecular Biology Laboratory
INSDC first public
2016-07-13T17:01:22Z
INSDC last update
2018-03-09T01:25:56Z
INSDC status
public
Submitter Id
E-MTAB-3585:cytRNA-Aniso-RIII
broker name
ArrayExpress
cell line
hek t293
common name
human
sample name
E-MTAB-3585:cytRNA-Aniso-RIII
scientific_name
Homo sapiens