Reclaimed site (Rec2 ecotype), frozen organic layer replicate 1
uncultured eukaryote
Sample of reclaimed boreal forest soil (ecotype Rec2 : peat over salvaged mineral soil lying above overburden (after >1m); placed 15 years before sampling), kept at -80°C until sequencing
ENA first public
2018-12-19
ENA last update
2017-12-21
External Id
SAMEA104448027
INSDC center alias
IBENS
INSDC center name
Institut de Biologie de Ecole normale superieure (IBENS), France
INSDC first public
2018-12-19T17:03:06Z
INSDC last update
2017-12-21T17:47:39Z
INSDC status
public
Submitter Id
Rec2-org_S10_L001
averaged total c
34.39
averaged total n
1.43
collection date
2013-07-16
environment (biome)
taiga (boreal forest)
environment (feature)
above Albertan bitumen ore deposit
environment (material)
forest soil
environmental sample
Yes
further details
http://unieuk.org/project/
geographic location (country and/or sea)
Canada
geographic location (latitude)
56.996253
geographic location (longitude)
-111.614222
geographic location (region and locality)
Northeastern Alberta
pcr primers
TAReuk454FWD1 (5′-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3'), TAReukREV3 (5′-ACTTTCGTTCTTGAT(C/T)(A/G)A-3′)
ph (topsoil)
5.1
sample collection device or method
Each site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal sEach site was sampled on 3 points located at 10 meters from each other to have 3 biological replicates. Each replicate was composed of four locations taken on the edges of a 10 cm square. At each location, the organic layer and the top 5 cm of mineral soil were sampled separately. About 100 mL of soil was collected for each replicate in a sterile Ziploc plastic bag, attempting not to disturb the internal structure of the sample.tructure of the sample.
sample name
Rec2-org_S10_L001
site
Rec2
soil layer
Organic
storage
frozen
target gene
18S
target subfragment
V4
treated
yes
treatment
reclamation 2, 15 years before sampling