C8866_INPUT_VTCM108
Gallus gallus
Protocols: Pectoralis major muscle samples were collected from 35 days old chickens. Sampling (approx. 800 mg) was performed in the middle of the tissue. Samples were snap frozen in liquid nitrogen. standard Cobb500 growth condition. TM: 39.5°C and 65% RH 12h/days from embryonic days E7 to E16 and the rest of the time 37,8°C 55% RH during embryogenesis. no treatment (Control): 37,8°C and 55% RH during embryogenesis. Muscle native chromatin immunoprecipitation (ChIP) was performed as described in David et al., 2017 (doi: 10.1186/s12575-017-0059-0). Antibodies batches used: Millipore 07-449#2506493 (H3K27me3); Millipore 07-473#2717639 (H3K4me3). Sequencing libraries were prepared using the NEB Next Ultra II DNA Library Prep kit for Illumina (New England Biolabs, Ipswich, USA). Ten ng of H3K27me3 IP DNA, 10 ng of H3K4me3 IP DNA and 100 ng of input DNA were used. Agencourt AMPure XP beads (Beckman Coulter, Brea, USA) were used for the 200 bp size selection of DNA fragments. Libraries concentrations were measured by Qubit and stored at −80 °C until sequencing.
ENA-FIRST-PUBLIC
2019-01-31T17:02:07Z
ENA-LAST-UPDATE
2018-10-16T14:23:41Z
External Id
SAMEA4976016
INSDC center name
BOA, INRA, Universite de Tours
INSDC first public
2019-01-31T17:02:07Z
INSDC last update
2018-10-16T14:23:41Z
INSDC status
public
Submitter Id
E-MTAB-7297:C8866_INPUT_VTCM108
age
35
broker name
ArrayExpress
common name
chicken
growth condition
control
organism part
pectoral muscle
sample name
E-MTAB-7297:C8866_INPUT_VTCM108
scientific_name
Gallus gallus
strain
Cobb500