6B_C-41512529
Homo sapiens
Protocols: Mucosal curettage samples and epithelial cell cultures (incubated with or without S. pneumoniae for 3 hours) were collected in RNALater (ThermoFisher) and stored at -80°C. RNA Extraction was performed using the RNEasy micro kit (Qiagen) and genomic DNA was removed with on column DNA digestion or with the Turbo DNA-free Kit (Qiagen). Extracted RNA quality was assessed and quantified using a BioAnalyser (Agilent 2100). Library preparation was performed using the Kappa Hyperprep kit (Roche Diagnostics) and sequencing was performed by the Pathogens Genomic Unit (UCL) on the Illumina Nextseq using the Nextseq 500/550 High Output 75 cycle kit (Illumina) according to manufacturers' instructions, giving 15-20 million 41bp paired end reads per sample.
ENA-FIRST-PUBLIC
2019-05-01T04:02:53Z
ENA-LAST-UPDATE
2019-04-03T09:42:43Z
External Id
SAMEA5547555
INSDC center name
Division of Infection and Immunity University College London
INSDC first public
2019-05-01T04:02:53Z
INSDC last update
2019-04-03T09:42:43Z
INSDC status
public
Submitter Id
E-MTAB-7841:6B_C-41512529
broker name
ArrayExpress
cell line
Detroit 562
cell type
epithelial cell
common name
human
developmental stage
adult
disease
pharyngeal cancer
infect
Streptococcus pneumoniae strain 6B
organism part
pharynx
phenotype
pneumococcal infection
sample name
E-MTAB-7841:6B_C-41512529
scientific_name
Homo sapiens