Sample 2
Mus musculus
Protocols: Alveolar macrophages of CD11c-Cre-ADAR1Δ7-9/Δ7-9 and CD11c-Cre-ADAR1wt/wt control mice were collected from BAL fluid and purified by CD11b MagniSort depletion and CD11c MagniSort enrichment. Whole RNA was isolated using the RNeasy Plus Micro Kit and stored at -80°C until further processing. Integrity of total RNA was assessed on the Bio-Rad Experion. Semi-automated library preparation was performed on the IP-Star (Diagenode) using the TruSeq Stranded mRNA Kit (Illumina). Each library was quantified and qualified on a Bioanalyzer 2100 (High Sensitivity DNA Kit, Agilent Technologies). Equimolar amounts of libraries were pooled and quantified by digital PCR using the QuantStudio™ 3D Digital PCR System (Life Technologies).
ENA first public
2018-12-11
ENA last update
2018-02-09
External Id
SAMEA104584920
INSDC center alias
Institute for Clinical Immunology and Transfusion Medicine Justus-Liebig-University Giessen
INSDC center name
Institute for Clinical Immunology and Transfusion Medicine Justus-Liebig-University Giessen
INSDC first public
2018-12-11T17:02:39Z
INSDC last update
2018-02-09T16:10:45Z
INSDC status
public
Submitter Id
E-MTAB-6477:Sample 2
broker name
ArrayExpress
cell type
alveolar macrophage
common name
house mouse
genotype
CD11c-Cre-ADAR1
organism part
lung
phenotype
wild type
sample name
E-MTAB-6477:Sample 2