Harvested RNA from immortalised human mammary epithelial (HMLE) cells before and after they had undergone EMT and after EMT with QKI knocked down by siRNAs
Homo sapiens
To assess whether circRNA production is regulated in EMT, we harvested RNA from immortalised human mammary epithelial (HMLE) cells before and after they had undergone EMT in response to treatment with TGF-β. The TGF-β-treated cells are stably mesenchymal with typical morphology and marker expression and are referred to as mesHMLE cells. RNA from two biological replicates of HMLE and mesHMLE cells as well as two biological replicates of mesHMLE cells with QKI stably knocked down by QKI specific siRNAs was subjected to deep sequencing, using library preparation procedures and bioinformatics pipelines designed to detect circRNAs.
ENA first public
2015-03-16
ENA last update
2015-01-16
External Id
SAMEA3209359
INSDC center alias
CENTRE FOR CANCER BIOLOGY
INSDC center name
CENTRE FOR CANCER BIOLOGY
INSDC first public
2015-03-16T17:04:53Z
INSDC last update
2015-01-16T00:14:29Z
INSDC status
public
Submitter Id
MsiQKI_polyAminus_rep1
common name
human
sample name
MsiQKI_polyAminus_rep1