BCM-300-mut rep3
Helicobacter pylori
Protocols: H. pylori strains were grown in liquid cultures as described in: Bubendorfer, S., Krebes, J., Yang, I., Hage, E., Schulz, T.F., Bahlawane, C., Didelot, X. and Suerbaum, S. (2016) Genome-wide analysis of chromosomal import patterns after natural transformation of Helicobacter pylori. Nat. Commun., 7, 11995. For RNA extraction, 5 ml of bacterial cells grown in liquid medium were pelleted (4°C, 6000 x g, 3 110 min), snap-frozen in liquid nitrogen and stored at -80 °C. Afterwards, bacterial pellets were disrupted with a FastPrep® FP120 Cell Disrupter (Thermo Savant) using Lysing Matrix B 2 ml tubes containing 0.1 mm silica beads (MP Biomedicals, Eschwege, Germany). Isolation of RNA was performed using the RNeasy kit (Qiagen, Hilden, Germany) and on-column DNase digestion with DNase I. A second DNase treatment was carried out using the TURBO DNA-free™ Kit (Ambion, Kaufungen, Germany). Total rRNA depletion was performed prior to cDNA synthesis using a RiboZero Kit (Illumina, Germany). cDNA library was performed with random primers.
ENA-FIRST-PUBLIC
2018-12-19T17:03:12Z
ENA-LAST-UPDATE
2018-08-22T10:24:22Z
External Id
SAMEA4851085
INSDC center name
Max von Pettenkofer Institute, Faculty of Medicine, LMU Munich
INSDC first public
2018-12-19T17:03:12Z
INSDC last update
2018-08-22T10:24:22Z
INSDC status
public
Submitter Id
E-MTAB-7162:BCM-300-mut rep3
broker name
ArrayExpress
genotype
JHP1050 mutant
growth condition
cell suspension culture
phenotype
GCGC-MTase JHP1050 mutant
sample name
E-MTAB-7162:BCM-300-mut rep3
scientific_name
Helicobacter pylori
strain
BCM-300