Spleen_WT+rLCMV-core_day7_r1
Mus musculus
Protocols: 5x10^6 naive CD8 T cells from Cor93 TCR transgenic mice (BC10.3) were transferred into C57BL/6 wild-type or MUP-core recipients. Mice were splenectomized and treated with anti-CD62L Abs 48 hours and 4 hours prior to cell transfer, respectively. Wild-type mice were injected with 2.5*10^5 infectious units of non-replicating rLCMV-core 4 hours prior CD8 T cell transfer. Livers were collected and analyzed at day 1, 3 and 7 after CD8 T cell transfer. ReliaPrep RNA MiniPrep System (Promega) RNA-Seq libraries were prepared with the SMART-Seq2 protocol, with minor modification. Briefly, RNA (1-5 ng) was reverse transcribed using custom oligodT and template-switching LNA oligos (sequences), followed by PCR amplification and clean-up (Ampure XP beads, Beckman Coulter). The resulting cDNA (0,5- 1 ng) was tagmented at 55°C for 30 minutes and final RNA-Seq libraries generated using reagents from the Nextera XT DNA Library Prep Kit (Illumina).
ENA-FIRST-PUBLIC
2019-08-10T04:03:21Z
ENA-LAST-UPDATE
2019-07-31T10:42:31Z
External Id
SAMEA5819766
INSDC center name
San Raffaele Telethon Institute for Gene Therapy.
INSDC first public
2019-08-10T04:03:21Z
INSDC last update
2019-07-31T10:42:31Z
INSDC status
public
Submitter Id
E-MTAB-74621564565901:Spleen_WT+rLCMV-core_day7_r1
age
8 to 10
broker name
ArrayExpress
cell type
CD8 T cell
common name
house mouse
developmental stage
adult
genotype
wild type genotype
growth condition
transplanted with naive CD8+ ve T cells from Cor93 TCR (BC10.3) transgenic mice
individual
1
infect
replication-defective lymphocytic choriomeningitis virus-core
organism part
spleen
sample name
E-MTAB-74621564565901:Spleen_WT+rLCMV-core_day7_r1
scientific_name
Mus musculus
sex
male
strain
C57BL/6