MCF10A siUSP22 2
Homo sapiens
Protocols: MCF10A cells were cultured in DMEM/F12 medium supplemented with 5% horse serum, 100 μg/ml EGF, 1 mg/ml hydrocortisone, 1 mg/ml Cholera toxin, 10 mg/ml Insulin, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. siRNA transfections were performed using Lipofectamine® RNAiMAX (Invitrogen) according to the manufacturer's instructions. RNA was extracted usiung QIAzol® Lysis Reagent (Qiagen) according to the manufacturer's protocol. Libraries were prepared using the TruSeq RNA Sample Preparation Kit (Illumina®) after verifying the RNA integrity on an agarose gel. Library quality was assessed using an Agilent Bioanalyzer 2100.
ENA-FIRST-PUBLIC
2019-12-06T04:04:39Z
ENA-LAST-UPDATE
2019-08-14T16:02:45Z
External Id
SAMEA5866261
INSDC center name
University Medical Center Gottingen Clinic for General, Visceral and Pediatric Surgery Tumor Epigenetics
INSDC first public
2019-12-06T04:04:39Z
INSDC last update
2019-08-14T16:02:45Z
INSDC status
public
Submitter Id
E-MTAB-8247:MCF10A siUSP22 2
broker name
ArrayExpress
cell line
MCF 10A
cell type
epithelial cell
common name
human
disease
normal
genotype
USP22 knockdown
organism part
mammary gland
sample name
E-MTAB-8247:MCF10A siUSP22 2
sampling time point
48
scientific_name
Homo sapiens