Arachis hypogaea
Seedlings  were grown in a greenhouse at 25~30 C. Leaf from 10~12 days of peanuts were  collected and prepared for explants.Leaf explants were cut with a blade to  generate wounds. Then they were immersed in A.rhizogenes (K599) suspension  (OD 0.5) for 10 min. The infected leaf-explants were dried on sterile filter  paper and transferred to the MS solid medium for 48 h in the dark. After  co-cultivation, the explants were sterilized with 250 mg.L-1 cefotaxime  (Gen-View Scientific, Inc., Florida, USA) for 5 min and washed with sterile  water for 3 times. Then all the explants were cultured on the MS solid medium  contained 250 mg.L-1 cefotaxime. The HRs were induced at the wound sites for  about 4 weeks.
isolate
Hair root
cultivar
Yue you 7
ecotype
Drought resistant tetraploid cultivars
age
>30 days
dev_stage
not applicable
geo_loc_name
China
tissue
Root
sample_type
mix root sample
treatment
control
BioSampleModel
Plant