C50336-dam-3
Mus musculus
J774A.1 cells infected with Salmonella Enteritidis C50336-dam-3
strain
BALB/cN
isolate
not applicable
breed
not applicable
cultivar
not applicable
ecotype
not applicable
age
not applicable
dev_stage
adult
sex
female
tissue
mouse blood
cell_line
J774A.1
cell_type
macrophage
sample_type
mixed culture
treatment
J774A.1 cells were seeded at a density of 5000000 cells per 10 cm dish. Overnight, cells were were pre-treated with 1 g/mL LPS for 5 h, and then infected with Salmonella Enteritidis C50336-dam-3 at an MOI of 20:1 and spun onto the cells at 1000 rpm for 10 min. Cells were incubated at 37 for 1 h, and subsequently washed twice, incubated for an additional 3 h with Opti-MEM containing 50 g/mL genttamicin. Then the cells were collected with 6 mL Trizol. The total RNA was isolated, purified, and quantified.
BioSampleModel
Model organism or animal