1762sTS.Chicken.Testis.5wph.Male
Gallus gallus
Protocols: The organs were dissected from 9 developmental stages (starting at e10 until P155). The dissected 'brain' samples correspond to the cerebral hemispheres and the 'cerebellum' samples to the cerebellum. In chicken only the left ovary develops and this was the one that was dissected. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina)
ENA-FIRST-PUBLIC
2018-12-29T17:01:47Z
ENA-LAST-UPDATE
2018-05-11T10:57:48Z
External Id
SAMEA4659412
INSDC center name
University of Heidelberg
INSDC first public
2018-12-29T17:01:47Z
INSDC last update
2018-05-11T10:57:48Z
INSDC status
public
Submitter Id
E-MTAB-6769:1762sTS.Chicken.Testis.5wph.Male
age
35
broker name
ArrayExpress
common name
chicken
developmental stage
postnatal
genotype
wild type genotype
organism part
testis
sample name
E-MTAB-6769:1762sTS.Chicken.Testis.5wph.Male
scientific_name
Gallus gallus
sex
male
strain
Red Junglefowl