wt.2_chip
Schizosaccharomyces pombe
Protocols: S. pombe cells were grown in EMM medium supplemented with 20 ug/ml Thiamine at 30C for 10 hours (final OD600: 0.5-0.6). Cells were harvested and fixed for 20 minutes with 1% formaldehyde (Larochelle et al. NAR 2012, 40:10240) Whole cell extract from fixed cells was prepared as described in (Larochelle et al. NAR 2012, 40:10240) ChIP experiments were performed as described in (Larochelle et al. NAR 2012, 40:10240). For ChIP-seq experiments, chromatin immunoprecipitation was done as described above with the following modifications. We scaled up the reactions to obtain larger amount of chromatin for library preparation. Chromatin was immunoprecipitated with Pan mouse IgG Dynabeads (Invitrogen) coated with antibody against the HA tag (12CA5, Roche). Libraries were prepared using the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina kit (New England BioLabs) according to the manufacturer instructions.
ENA-FIRST-PUBLIC
2014-09-20T17:03:04Z
ENA-LAST-UPDATE
2018-03-08T17:08:16Z
External Id
SAMEA2335643
INSDC center name
MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
INSDC first public
2014-09-20T17:03:04Z
INSDC last update
2018-03-08T17:08:16Z
INSDC status
public
Submitter Id
E-MTAB-2237:wt.2_chip
broker name
ArrayExpress
common name
fission yeast
genotype
h- rpb3-3HA::KanMX6
sample name
E-MTAB-2237:wt.2_chip
scientific_name
Schizosaccharomyces pombe
sex
mating_type_h_minus
strain
FBY-1464