Sample 8
Homo sapiens
Protocols: Biopsy was obtained from the patient under informed consent in DMEM F12 containing 10% FBS and 2% P/S and processed within 2 hours. The tissue was minced into small pieces and washed extensively with PBS before being dissociated with collagenase IV for 2-3 hours and additional 15' with TripLE at 37C. Digestion was stopped by medium dilution and the pellet was resuspended in 70% Matrigel. Organoids were cultured in endometrial cancer organoids culture medium. Organoids were recovered from Matrigel with ice-cold DMEM F12 and centrifuged at 230g for 5'. The pellet was resuspended in 200ul of PBS and genomic DNA was isolated using the Purelink Genomic Mini Kit (Invitrogen) according to the manufacturer's instructions. DNA concentration was measured with a nanodrop and DNA was stored at -80C before being processed for exome sequencing. Whole genome DNA libraries were created using the Illumina TruSeq DNA sample preparation kit V2 according to the manufacturer's instructions and whole-exome capture was performed using the Nimblegen SeqCap EZ Developer Library kit.
ENA-FIRST-PUBLIC
2019-06-04T17:04:08Z
ENA-LAST-UPDATE
2019-02-18T16:06:32Z
External Id
SAMEA5358962
INSDC center name
VIB Center for Cancer Biology (CCB) Laboratory for Translational Genetics Department of Human Genetics KULeuven
INSDC first public
2019-06-04T17:04:08Z
INSDC last update
2019-02-18T16:06:32Z
INSDC status
public
Submitter Id
E-MTAB-7688:Sample 8
broker name
ArrayExpress
common name
human
developmental stage
primary
disease
endometrium cancer
disease staging
IA
individual
7
organism part
endometrium
sample name
E-MTAB-7688:Sample 8
scientific_name
Homo sapiens
sex
female
tumor grading
II