LR3
Bos taurus
Protocols: Oocytes were collected from cows using transvaginal ultrasound-guided ovum pick-up (OPU). Using an ultrasound probe system (General Electric) with a cannula connected to an aspiration pump (Minitube), subordinate follicles of size smaller than 8mm were aspirated. Oocytes were mechanically denuded using hyaluronidase and snap frozen. Pooled oocytes were lysed and bisulfite treated using the EZ-DNA Methylation-Direct Kit (Zymo Research) following manufacturer's instructions. Sequencing libraries were generated from the bisulfite treated DNA using the Pico Methyl-Seq Library Prep Kit (Zymo) according to manufacturer's recommendations with 20µM PrepAmp primer mix and 10-cycle PCR amplification.
ENA-FIRST-PUBLIC
2020-07-23T04:03:27Z
ENA-LAST-UPDATE
2019-07-26T16:53:37Z
External Id
SAMEA5816290
INSDC center name
Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit
INSDC first public
2020-07-23T04:03:27Z
INSDC last update
2019-07-26T16:53:37Z
INSDC status
public
Submitter Id
E-MTAB-8191:LR3
broker name
ArrayExpress
common name
cattle
developmental stage
adult
organism part
oocytes
sample name
E-MTAB-8191:LR3
scientific_name
Bos taurus
sex
female
study group
9-10 week post-partum cows
breed
Holstein-Friesian