Sample 6
Mus musculus
Protocols: Isolation of fibroblasts from C57BL/6 animals by digestion of colonic tissue with DNase I, Collagenase D and Dispase I for 15minutes at 37°C. Filtered cells were grown in cell culture flasks and adhereing fibrobalsts grew to confluence within some days. Cultivation of murine fibroblasts was done with DMEM F12 (10% FCS, 1% Pen/Strep). Stimulation was performed pairwise (3 stimulated vs 3 unstimulated) for 9 days. Stimulation was performed with 3x10E5 cells per well in a 6 well plate with 2ml medium with IL-36R ligands (33.3ng/ml IL-36a, 33.3ng/ml IL-36b, 33.3ng/ml IL-36g) for 9 days. As control PBS was used. Fresh stimulation was added every second day. RNA isolated was performed according to the manufacturer's protocol (Macherey-Nagel, Nucleo Spin RNA) including an additional DNAse digestion step as recommended. Libraries were constructed Illumina Stranded mRNA Kit
ENA first public
2018-10-31
ENA last update
2018-02-12
External Id
SAMEA104588905
INSDC center alias
AG Neufert Medical clinic 1, Friedrich-Alexander Universitat Erlangen-Nurnberg
INSDC center name
AG Neufert Medical clinic 1, Friedrich-Alexander Universitat Erlangen-Nurnberg
INSDC first public
2018-10-31T17:02:05Z
INSDC last update
2018-02-12T14:26:47Z
INSDC status
public
Submitter Id
E-MTAB-6476:Sample 6
age
12
broker name
ArrayExpress
cell type
fibroblast
common name
house mouse
disease
normal
genotype
wild type genotype
individual
3
organism part
colon
sample name
E-MTAB-6476:Sample 6
sex
male
strain
C57BL/6