nuc_nucRNA-Aniso-RII
Homo sapiens
Protocols: Cells were seeded 48 hours prior to stress treatment. Cells were subsequently treated with 5 ??g/ml Anisomycin for 3 hours and harvested in PBS. Total RNA was extracted using Trizol LS. Cells were fractionated and nuclear RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions. mRNA-Seq libraries (from polyA+ cytoplasmic RNA) were created according to the standard Illumina TruSeq protocol.
ENA-FIRST-PUBLIC
2016-07-13T17:01:22Z
ENA-LAST-UPDATE
2018-03-09T01:25:26Z
External Id
SAMEA3403319
INSDC center name
European Molecular Biology Laboratory
INSDC first public
2016-07-13T17:01:22Z
INSDC last update
2018-03-09T01:25:26Z
INSDC status
public
Submitter Id
E-MTAB-3585:nuc_nucRNA-Aniso-RII
broker name
ArrayExpress
cell line
hek t293
common name
human
sample name
E-MTAB-3585:nuc_nucRNA-Aniso-RII
scientific_name
Homo sapiens