Sample 18
Homo sapiens
Protocols: LNCAP cells were seeded at an appropriated density and dosed 24 hours later with 1uM of each compound. Either 24 or 48 hours later cells were lysed in RLT buffer (Qiagen) on ice; adherent cells were scraped and the material was passed through a QIAshredder (Qiagen). Samples were then snap frozen on dry ice and stored at -80ºC. Samples were randomised and extracted on Qiacube-HT following the RNeasy 96 Qiacube HT total RNA Cell with DNase protocol. RNA concentration and quality was assessed with Qubit RNA BR kit (Invitrogen) and Agilent RNA 6000 Nano kit. Libraries were prepared using the TruSeq Stranded mRNA library preparation kit as per the manufacturers guidelines
ENA-FIRST-PUBLIC
2019-03-31T17:01:57Z
ENA-LAST-UPDATE
2018-10-12T09:25:53Z
External Id
SAMEA4971986
INSDC center name
AZ
INSDC first public
2019-03-31T17:01:57Z
INSDC last update
2018-10-12T09:25:53Z
INSDC status
public
Submitter Id
E-MTAB-7294:Sample 18
broker name
ArrayExpress
cell line
LNCAP
common name
human
disease
prostate carcinoma
organism part
prostate
sample name
E-MTAB-7294:Sample 18
scientific_name
Homo sapiens