CT-393-HT-3HR-1
Gallus gallus
Protocols: Samples were collected 3-hour, 2-weeks, and 4-week post heat from both control (unheated) and treatment (heated) birds. Samples were immediately placed in RNAlater post collection. Daily heat cycles consisting of 7 hours of elevated temperature of 35°C and 17 hours of ambient temperature of 30°C for treatment birds. Control birds remained at 23°C throughout the entire experiment. RNAqueous Total RNA Isolation Kit following manufacturer's standard protocol and treated with Ambion DNA-free DNase Treatment and Removal Reagents following manufacturer's standard protocol. Illumina TruSeq Stranded mRNA Library Prep Kit following the manufacturer's standard protocol.
ENA-FIRST-PUBLIC
2019-05-01T04:02:54Z
ENA-LAST-UPDATE
2019-04-11T17:11:29Z
External Id
SAMEA5564240
INSDC center name
IOWA STATE UNIVERSITY
INSDC first public
2019-05-01T04:02:54Z
INSDC last update
2019-04-11T17:11:29Z
INSDC status
public
Submitter Id
E-MTAB-7865:CT-393-HT-3HR-1
age
24
breed
W-36
broker name
ArrayExpress
common name
chicken
developmental stage
adult
genotype
wild type genotype
individual
CT393
organism part
cecal tonsil
sample name
E-MTAB-7865:CT-393-HT-3HR-1
scientific_name
Gallus gallus
sex
female