Sample 1
Euglena agilis
Protocols: We cultured E. agilis in mAC, AF-6, and C media for mRNA preparation. E Each culture was maintained in 100 mL volume test tubes for more than 3 days using 50 mL of either culture media type at 29°C with aeration (50 mL/min) and constant illumination (100 μmol photon/m2s). Total RNA was extracted from cells using NucleoSpin RNA (Macherey-Nagel, USA). A sequencing library was constructed using a TruSeq Stranded mRNA LT Sample Preparation Kit (Illumina, Inc.) according to the manufacturer's instructions (TruSeq Stranded mRNA Sample Preparation Guide Rev. E; Illumina, Inc.).
ENA first public
2018-12-14
ENA last update
2018-01-10
External Id
SAMEA104459099
INSDC center alias
Center for Sustainable Resource Science, RIKEN
INSDC center name
Center for Sustainable Resource Science, RIKEN
INSDC first public
2018-12-14T17:01:51Z
INSDC last update
2018-01-10T13:23:14Z
INSDC status
public
Submitter Id
E-MTAB-6349:Sample 1
broker name
ArrayExpress
organism part
whole organism
sample name
E-MTAB-6349:Sample 1
strain
NIES-3945