OS25_UD
Mus musculus
source_name
OS25 ES cells
growth
ESCs were cultured were grown on mitomycin C-treated primary embryonic fibroblasts (PEFs) derived from E12.5 mouse embryos in DMEM supplemented with 15% fetal calf serum (FCS) , 1,000 units/ml LIF, nonessential amino acids, sodium pyruvate, 2-mecaptoethanol, L-glutamine, and Penicillin/Streptomycin.
treatment
OS25 ES cells were cultured and differentiated for 3 days (2 days with RA). Differentiation was induced by plating the cells at low density without LIF or hyg for 1 day. Retinoic acid (RA) 5×10-6 M was then added.
cell type
ES cell