Sample 5
Lilium longiflorum
Vernalized for 5 weeks at 4C Protocols: L. longiflorum bulbs (cultivar White Heaven) were obtained from a nursery at the end of the growing season (August). Bulbs were sanitized and stored in humid standard pot medium at 25C (control, 0W) or at 4C for 2, 5, 7 or 9 weeks (2W, 5W, 7W, 9W, respectively). At each of these time points, shoot apical meristems were excised from the bulbs, immediately frozen in liquid nitrogen and then stored at -80C until RNA extraction. RNA was isolated using the AurumTM Total RNA mini kit (Biorad) following manufacturers instructions. The procedure was followed by an additional DNAse treatment with TURBO DNase (Life technologies) to remove all genomic DNA still present after the isolation. The RNA integrity was confirmed using the 2100 Bioanalyzer (Agilent Technologies). The samples were prepared for transcriptome sequencing using the Illumina kit (TruSeq RNA Sample Preparation Kit v2) following the manufacturers recommendations.
ENA-FIRST-PUBLIC
2015-03-17T17:02:51Z
ENA-LAST-UPDATE
2018-03-08T19:39:38Z
External Id
SAMEA2697752
INSDC center name
Genetwister Technologies B.V.
INSDC first public
2015-03-17T17:02:51Z
INSDC last update
2018-03-08T19:39:38Z
INSDC status
public
Submitter Id
E-MTAB-2825:Sample 5
broker name
ArrayExpress
common name
trumpet lily
organism part
apical shoot meristem
sample name
E-MTAB-2825:Sample 5
scientific_name
Lilium longiflorum