U-343_MGa_shPROX1_P6104_210
Homo sapiens
Protocols: Glioblastoma cell lines cultured in vitro PROX1 transduction in U-343 MG was performed using commercial lentiviral particles. Cells were selected using Puromycin. PROX1 stable knockdown in cell line U-343-MGa was performed using lentiviral particles produced in house. Lentiviral vectors were transduced in HEK-293T cells using calcium-phosphate co-transfection. 24 hours after transfection supernantants were collected two times within 24-hour interval, filtered and then used to infect U-343 MGa cells. Cell were further selected with Puromycin antibiotic. Total RNA was purified using the PureLink® kit following the manufacturer's instructions (Thermo Fisher Scientific) Illumina TruSeq Stranded mRNA with Poly-A selection
ENA-FIRST-PUBLIC
2018-12-29T17:01:49Z
ENA-LAST-UPDATE
2018-07-26T15:59:47Z
External Id
SAMEA4805907
INSDC center name
SciLifeLab, Division of Genome Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 21 Stockholm, Sweden
INSDC first public
2018-12-29T17:01:49Z
INSDC last update
2018-07-26T15:59:47Z
INSDC status
public
Submitter Id
E-MTAB-6991:U-343_MGa_shPROX1_P6104_210
broker name
ArrayExpress
cell line
U-343_MGa
cell type
epithelial cell
common name
human
disease
glioblastoma
genotype
wild type genotype
organism part
brain
phenotype
shRNA knockdown of PROX1
rna interference
shRNA against PROX1 mRNA
sample name
E-MTAB-6991:U-343_MGa_shPROX1_P6104_210
scientific_name
Homo sapiens