L3.6_pl_sicontrol_rep_3
Homo sapiens
Protocols: Cells were transfected with siControl and sip63 using Lipofectamine® RNAiMAX (Invitrogen) according to the manufacturer's instructions. Triplicates for each condition were harvested 48 hours after transfection by QIAzol® reagent (Qiagen) according to manufacturer's instructions. Reverse transcription of 1 µg RNA was performed using M-MuLV reverse transcriptase (NEB) and random primers. The quality of RNA from L3.6pl and BxPC-3 with a triplicate for siControl condition and sip63 were checked by loading on a 1% agarose gel. Libraries for RNA were prepared using the TruSeq RNA Library Prep Kit V2 (Illumina) according to the manufacturer's instructions.
ENA-FIRST-PUBLIC
2018-12-18T17:02:52Z
ENA-LAST-UPDATE
2018-07-19T16:55:22Z
External Id
SAMEA4799040
INSDC center name
Department of General, Visceral and Pediatric Surgery, University Medical Center Gottingen, 37075 Gottingen, Germany
INSDC first public
2018-12-18T17:02:52Z
INSDC last update
2018-07-19T16:55:22Z
INSDC status
public
Submitter Id
E-MTAB-7033:L3.6_pl_sicontrol_rep_3
broker name
ArrayExpress
cell line
L3.6pl
cell type
epithelial cell
common name
human
disease
pancreatic adenosquamous carcinoma
metastatic site
celiac lymph node
organism part
pancreas
sample name
E-MTAB-7033:L3.6_pl_sicontrol_rep_3
scientific_name
Homo sapiens