FeDefKO3
Mus musculus
Protocols: For temporal, hepatocyte-specific disruption of hepcidin, mouse floxed for Hamp1 (Hampfl/fl) on a C57BL/6 background were crossed with mice harboring CreERT2 recombinase under the control of the serum albumin promoter (AlbCreERT2) to generate AlbCreERT2;Hampfl/fl mice. Wild-type littermates were used as controls for these respective experiments (Hampfl/fl). Mice were injected with tamoxifen (Sigma-Aldrich, St. Louis, MO) at 100 mg/kg of body weight via i.p. for three consecutive days to ensure Cre-mediated recombination. All mice were fed ad libitum and kept in a 12 hour dark/light cycle. All mice were fed with either a purified AIN-93G iron-replete (350 PPM) or low-iron (< 5 PPM) diet (Dyets, Bethlehem, PA) for two weeks prior to sacrifice. All mice were housed at the Unit for Laboratory Animal Management (ULAM) at the University of Michigan The small intestine tissue was scraped using a glass microscope slide and bead homogenized in Trizol (Thermo Fisher, Waltham, MA). RNA was extracted using chloroform (Sigma-Aldrich, St. Louis, MO) and isopropanol (Sigma-Aldrich, St. Louis, MO) RNA quality was assessed by the Agilent Bioanalyzer 2100. RNA sequencing libraries were prepared using the TruSeq RNA library prep kit v2 (Illumina, San Diego, CA) following the manufacturer's recommended protocol.
ENA-FIRST-PUBLIC
2018-12-12T17:01:47Z
ENA-LAST-UPDATE
2018-10-23T16:08:02Z
External Id
SAMEA5041597
INSDC center name
Department of Environmental Health Sciences University of Michigan School of Public Health
INSDC first public
2018-12-12T17:01:47Z
INSDC last update
2018-10-23T16:08:02Z
INSDC status
public
Submitter Id
E-MTAB-7329:FeDefKO3
age
3
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
AlbCreERT2;Hamp[fl/fl]
organism part
duodenum
phenotype
hepatocyte-specific Hamp1 knockout
sample name
E-MTAB-7329:FeDefKO3
scientific_name
Mus musculus
sex
female
strain
C57BL/6