unc-75 (yb1701) L1 nucleoplasmic fraction [RNAseq]
Caenorhabditis elegans
The worm strain used was KH1752. This is a mutant worm strain. The genotype is unc-75 (yb1701) I. Embryos were obtained by bleaching synchronized gravid adults. The embryos were incubated for 18 hours at 20 degree Celsius in M9 buffer. The synchronized L1 worms were then fed with E. coli strain OP50 suspended in S-complete medium for 3 hours at 20 degree Celsius. The L1 worms were cleaned by washing with the M9 buffer and total homogenate was prepared by grinding the worms in liquid nitrogen. Nuclei were harvested by centrifuging the total homogenate. Nucleoplasmic fraction (clear supernatant) was prepared by treating the nuclei with Nuclear Lysis Buffer followed by centrifugation. Total RNAs were extracted from the nucleoplasmic fraction by using Sepasol I Super (nacalai tesque) and then purified by using RNeasy Plus Mini kit plus DNase I (Qiagen).
sample_name
4_unc-75_L1_Nucleoplasm
strain
KH1752
biomaterial_provider
Hidehito KUROYANAGI
collection_date
2014-03-18
dev_stage
L1
genotype
unc-75 (yb1701) I
sex
hermaphrodite