cytRNA-DMSO-R-II
Homo sapiens
Protocols: Cells were seeded 48 hours prior to treatment. DMSO was subsequently added to cells as a solvent control. Cells were harvested in PBS 3 hours later. Total RNA was extracted using Trizol LS. Cells were fractionated and cytoplasmic RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions. 3'T-fill libraries were created as described in Wilkening S, et al. An efficient method for genome-wide polyadenylation site mapping and RNA quantification. Nucleic Acids Research. 2013;41(5):e65. doi:10.1093/nar/gks1249.
ENA-FIRST-PUBLIC
2016-07-13T17:01:22Z
ENA-LAST-UPDATE
2018-03-09T01:25:26Z
External Id
SAMEA3403313
INSDC center name
European Molecular Biology Laboratory
INSDC first public
2016-07-13T17:01:22Z
INSDC last update
2018-03-09T01:25:26Z
INSDC status
public
Submitter Id
E-MTAB-3585:cytRNA-DMSO-R-II
broker name
ArrayExpress
cell line
hek t293
common name
human
sample name
E-MTAB-3585:cytRNA-DMSO-R-II
scientific_name
Homo sapiens