S01-T31
Brassica napus
N- 1d root
isolate
not collected
cultivar
Zhongshuagn11(ZS11)
ecotype
wild type
age
seeding
dev_stage
Five-leaf stage
geo_loc_name
China:Chongqing
tissue
Roots
biomaterial_provider
Hai Du
growth_protocol
Seeds of Brassica napus cultivar Zhongshuagn11(ZS11) were obtained from the College of Agriculture and Biotechnology, Southwest University (Chongqing, China) and grown in soil (nutrition soil:rock = 2:1) in a plant incubator under the long-day conditions (day/night = 16/8h). Seedlings with four expanded leaves (four-leaf stage) were transferred to N-street liquid medium, and were grown in an artificial climate chamber at 25°C under a 16/37h (day/night) photoperiod.
treatment
After the preculture, two sets of plants were cultivated with two different condition: optimal nitrogen nutrition (control) including 1.8 mM Ca(NO3)2 and 0.2 mM (NH4)2SO4, and Nitrogen stress condition, with 0.1 mM Ca(NO3)2 and 1 mM CaSO4 to satisfy the optimum Ca nutrition (Koeslin-Findeklee et al., 2015). Besides, 10 µM C2H4N4 (dicyandiamide) was used to prevent nitrification. The whole root system was maintained submersed in the nutrient solution, which was replaced every day. All plants were cultivated to V5 (five unfolded leaves) by the nutrient solution. At this period, the leaves and roots of ZS11 were sampled with two biological replicates at 1, 3, 5, 7 and 41 day
BioSampleModel
Plant