D14_EMSF-2
Homo sapiens
Protocols: Embryoid bodies (EB) generated from hiPSC were cultured with defined chemical cocktails including WCP activator CHIR99021. Primary endometrial stromal fibroblasts (EMSF) were isolated from endometrial tissue. SUSD+ cells were isolated from primary EMSF using flow cytometry. Embryoid bodies (EB) generated from hiPSC were cultured with defined chemical cocktails including WCP activator CHIR99021. Total RNAs were isolated from hiPSC, D2.5, D4, D6, D8, D14, D22 VC, D22 IVD EB, and primary EMSF using the RNeasy Mini Kit (Qiagen, Germantown, MD). RNA-seq libraries were constructed from ~500 ng DNAse-treated total RNA using KAPA Stranded RNA-Seq Kit with RiboErase (KAPA Biosystems, Wilmington, MA).
ENA-FIRST-PUBLIC
2018-10-31T17:02:13Z
ENA-LAST-UPDATE
2018-10-12T11:13:40Z
External Id
SAMEA4972086
INSDC center name
Northwestern University Feinberg School of Medicine
INSDC first public
2018-10-31T17:02:13Z
INSDC last update
2018-10-12T11:13:40Z
INSDC status
public
Submitter Id
E-MTAB-7292:D14_EMSF-2
broker name
ArrayExpress
cell line
ACS-1028
cell type
endometrial stromal fibroblast
common name
human
developmental stage
adult
growth condition
embryoid body
organism part
bone marrow
progenitor cell type
induced pluripotent stem cell
sample name
E-MTAB-7292:D14_EMSF-2
scientific_name
Homo sapiens
sex
female