Sample 11
Mus musculus
Protocols: Primary fibroblasts were derived from adult CAST/EiJ x C57/Bl6 mice. Tail explants were cultured in fibroblast medium (DMEM high glucose, 10% ES cell FBS, 1% Penicillin/Streptomycin, 1% non-essential amino acids, 1% sodium-puryvate, 0.1mM b-Mercaptoethanol). Cells were passaged twice before distributed as individual cells into wells of 384 or 96 well plates using a FACS. Wells was pre-loaded with lysis buffer. Cell lysis Smart-seq2, indexed by Nextera i5 and i7, and without exogenous spike-ins.
ENA-FIRST-PUBLIC
2018-12-19T17:02:07Z
ENA-LAST-UPDATE
2018-07-30T10:06:03Z
External Id
SAMEA4808129
INSDC center name
Karolinska Institutet, Department of Cell and Molecular Biology
INSDC first public
2018-12-19T17:02:07Z
INSDC last update
2018-07-30T10:06:03Z
INSDC status
public
Submitter Id
E-MTAB-6385:Sample 11
age
8 to 14
broker name
ArrayExpress
cell type
fibroblast
common name
house mouse
developmental stage
adult
genotype
wild type genotype
organism part
dermis
sample name
E-MTAB-6385:Sample 11
sampling site
tail
scientific_name
Mus musculus
sex
female
strain
C57BL/6 x CAST/EiJ