Adult pancreas islets
Mus musculus
Protocols: Isolated islets were GFP sorted using mouse lines that expressed GFP with a minimal Pdx1 promoter. No MARIS sorting. After sorting, cells were pelleted by centrifugation at 3000 g for 5min at 4C. The supernatant was discarded. Total RNA was isolated from the pellet using the RecoverAll Total Nucleic Acid Isolation kit (Ambion), starting at the protease digestion stage of manufacturer-recommended protocol. The following modification to the isolation procedure was made: instead of incubating cells in digestion buffer for 15 minutes at 50C and 15 minutes at 80C, we carried out the incubation for 3 hours at 50C. Cell lysates were frozen at -80C overnight before continuing the RNA isolation by the manufacturer's instructions. NA
ENA-FIRST-PUBLIC
2015-03-17T16:40:38Z
ENA-LAST-UPDATE
2018-03-08T23:43:19Z
External Id
SAMEA3307737
INSDC center name
Massachusetts Institute of Technology
INSDC first public
2015-03-17T16:40:38Z
INSDC last update
2018-03-08T23:43:19Z
INSDC status
public
Submitter Id
E-MTAB-3417:Adult pancreas islets
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
organism part
islet
sample name
E-MTAB-3417:Adult pancreas islets
scientific_name
Mus musculus
strain or line
Pdx1 minimal promoter-GFP on mixed/outbred background