SW837 siNT5 4
Homo sapiens
Protocols: SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. siRNA transfections were performed using Lipofectamine® RNAiMAX (Invitrogen) according to the manufacturer's instructions. RNA was extracted usiung QIAzol® Lysis Reagent (Qiagen) according to the manufacturer's protocol. Libraries were prepared using NEXTflex™ Rapid Illumina Directional RNA-Seq Library Prep Kit (Illumina®) after verifying the RNA integrity on an agarose gel. Library quality was assessed using an Agilent Bioanalyzer 2100.
ENA-FIRST-PUBLIC
2019-12-06T04:04:39Z
ENA-LAST-UPDATE
2019-08-05T10:23:14Z
External Id
SAMEA5848836
INSDC center name
University Medical Center Gottingen Clinic for General, Visceral and Pediatric Surgery Tumor Epigenetics
INSDC first public
2019-12-06T04:04:39Z
INSDC last update
2019-08-05T10:23:14Z
INSDC status
public
Submitter Id
E-MTAB-8215:SW837 siNT5 4
broker name
ArrayExpress
cell line
SW837
common name
human
disease
colorectal adenocarcinoma
genotype
wild type genotype
organism part
colon
phenotype
wild type phenotype
sample name
E-MTAB-8215:SW837 siNT5 4
scientific_name
Homo sapiens