Hypocreales sp. ML197-S3
strain
ML197-S3
isolation_source
coastal sea water
collection_date
2015-08-01/2015-08-08
env_broad_scale
mediterranean sea biome [ENVO_01000047]
env_local_scale
environment associated with an aquatic invertebrate [ENVO_01001176]
env_medium
Protula[NCBITaxon_51281]|coastal sea water [ENVO_00002150]
geo_loc_name
Spain:Granada
host
Protula
isol_growth_condt
PMID: 32392868
lat_lon
36.723083 N 3.726417 W
host_body_habitat
Protula intestinum, Aphia ID: 131032
samp_collect_device
host collection: scuba diving
samp_mat_process
After sampling, the invertebrate was transferred carefully to the laboratory to be processed. Small pieces of tissue were taken from the sample before freezing. 1 cm3 samples were ground in sterile seawater and heated at 50C for 1 h. The suspension was serially diluted, plated on Difco Marine Broth Agar (BD Biosciences San Jose, CA, USA), and incubated at 28C for six weeks. A single colony was picked from the agar and cultivated as a pure culture on Difco Potato Dextrose Agar and Difco Marine Broth Agar (BD Biosciences San Jose, CA, USA) (media and cultivated for five days at 28C. The strain spores and mycelium were recovered by a gentle scratch of the agar plate surface using a scalpel, and they were conserved at 20C in 10% glycerol solution.
samp_salinity
sea water salinity: 38
samp_store_temp
host tissue: -20C, fungal isolate: -80C
temp
sea water temperature: 21C - 27C
BioSampleModel
MIGS.eu
BioSampleModel
MIGS/MIMS/MIMARKS.host-associated