DBal_MP_CROSS
Drosophila melanogaster
Protocols: Male double balancer flies (w; If/CyO; Sb/TM3,Ser) and female virginizer flies were crossed in vials. Adults (at least 20) from the F1 generation with the (+/CyO; +/TM3) phenotype were selected and snap-frozen in liquid nitrogen. Flies were grinded in liquid nitrogen with a pestle in a pre-cooled mortal. Grinded flies were transferred to an Eppendorf tube and resuspended in 400 microl of Buffer A (100 mM Tris-HCl pH7.5, 100 mM EDTA, 100 mM NaCl, 1% SDS). The sample was incubated at 65C for 30 min, then spun for 15 min at room temperature. The supernatant was transferred to a new tube and incubated at 37C for 45 min with 5 microl of 100 mg/ml of RNaseA. The DNA was extracted by two rounds of standard phenol-chloroform extraction, precipitated with ethanol, and resuspended in 150 microl of TE buffer. Libraries were prepared following instructions of the Nextera Mate Pair Sample Preparation Kit (Illumina).
ENA-FIRST-PUBLIC
2019-05-02T17:03:59Z
ENA-LAST-UPDATE
2019-05-02T08:39:09Z
External Id
SAMEA5587535
INSDC center name
EMBL
INSDC first public
2019-05-02T17:03:59Z
INSDC last update
2019-05-02T08:39:09Z
INSDC status
public
Submitter Id
E-MTAB-7510:DBal_MP_CROSS
broker name
ArrayExpress
common name
fruit fly
developmental stage
adult
genotype
+/CyO; +/TM3
organism part
whole organism
sample name
E-MTAB-7510:DBal_MP_CROSS
scientific_name
Drosophila melanogaster
sex
mixed sex
strain
F1CyOTM3