Sample 4
Mus musculus
Protocols: Mouse aorta was first digested for 1 hour at 37°C in 1 mg/mL collagenase I (Gibco, Life Technologies), 1 mg/mL dispase (Gibco, Life Technologies), 150 µg/mL DNase-I (Sigma-Aldrich) before passing the suspension through a 70 µmol/L cell strainer. Lineage+ cells were then depleted from tumor cell suspensions via MACS cell separation according to the manufacturer's instructions (Miltenyi Biotech). Cell number and viability for each sample was assessed by using 0.4% Trypan blue solution and a hemocytometer. Single-cell suspensions were then used for flow sorting or analysis by flow cytometry. ENSG ids were converted to gene symbols using tximport. RNA was extracted from sorted endovascular progenitor (EVP), transit amplifying (TA), and definitive differentiated (D) cells using a QIAGEN mini kit (Qiagen) according to the manufacturer's instructions. RNA quality and concentration was assessed by using the Agilent 2100 Bioanalyser (Agilent Technologies). Five to 100 ng of RNA was used for cDNA synthesis using the Superscript III Reverse Transcription Kit (Invitrogen). Total isolated RNA of appropriate RNA integrity number (>4) was prepared using the TruSeq stranded total RNA library preparation kit as per the manufacturer's instructions.
ENA-FIRST-PUBLIC
2019-03-31T17:01:55Z
ENA-LAST-UPDATE
2018-08-14T13:33:54Z
External Id
SAMEA4828310
INSDC center name
Division of Genomics of Development and Disease, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia, 4072
INSDC first public
2019-03-31T17:01:55Z
INSDC last update
2018-08-14T13:33:54Z
INSDC status
public
Submitter Id
E-MTAB-7148:Sample 4
age
8 to 12
broker name
ArrayExpress
cell type
aortic endothelial cell
common name
house mouse
disease
normal
genotype
wild type genotype
organism part
aorta
phenotype
CD31_hi/VEGFR2_hi
sample name
E-MTAB-7148:Sample 4
scientific_name
Mus musculus
sex
female
strain
C57BL/6