Homo sapiens
Protocols: Tumor cells from primary biopsies were grown as 2D cultures from 3 to 5 passages and harvested for RNA extraction Total RNA from cells was extracted by the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions. Sequencing libraries were constructed starting from 50-100ng of total RNA by optimizations of the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen GmbH).
ENA-FIRST-PUBLIC
2020-09-29T17:07:48Z
ENA-LAST-UPDATE
2020-09-25T17:49:37Z
External Id
SAMEA7340119
INSDC center name
Department of Experimental Oncology, IEO, European Institute of Oncology IRCSS, Milan, Italy
INSDC first public
2020-09-29T17:07:48Z
INSDC last update
2020-09-25T17:49:37Z
INSDC status
public
Submitter Id
E-MTAB-9605:16-AS-013
broker name
ArrayExpress
cell type
2D-cultured ascites
common name
human
disease
high grade ovarian serous adenocarcinoma
sample name
E-MTAB-9605:16-AS-013
scientific_name
Homo sapiens
sex
female
specimen with known storage state
fresh specimen