Mus musculus
Protocols: 4T1 cells (5×105 cells) were re-suspended in 50 µl growth factor-reduced Matrigel (Corning) and injected into the fourth mammary fat pads of female syngeneic BALB/cJ mice (8-week-old, n = 3). At day 10 (pre-metastatic stage), the mice were euthanized and then CD45+CD11b+Ly6GhighLy6Cmed neutrophils from bone marrow (BM), peripheral blood (PB) and lung were isolated by fluorescence-activated cell sorting. 4T1 cells were injected into the fourth mammary fat pads of female syngeneic BALB/cJ mice (8-week-old, n = 3). At day 10 (pre-metastatic stage), the mice were euthanized and then CD45+CD11b+Ly6GhighLy6Cmed neutrophils from bone marrow (BM), peripheral blood (PB) and lung were isolated by fluorescence-activated cell sorting. Total RNA was isolated from neutrophils using the miRNeasy Mini kit (Qiagen). Stranded libraries were prepared using the KAPA mRNA HyperPrep Kit (KAPA Biosystems): isolation of polyA containing mRNA using oligo-dT magnetic beads, RNA fragmentation, first and second strand cDNA synthesis, ligation of Illumina-specific adapters containing a unique barcode sequence for each library, and PCR amplification. Libraries were checked for quality and concentration using the DNA 1000 assay (Agilent Technologies) and quantitative PCR (KAPA Biosystems), according to the manufacturers' instructions.
ENA-FIRST-PUBLIC
2020-07-24T04:05:43Z
ENA-LAST-UPDATE
2020-05-28T09:41:12Z
External Id
SAMEA6863229
INSDC center name
THE JACKSON LABORATORY
INSDC first public
2020-07-24T04:05:43Z
INSDC last update
2020-05-28T09:41:12Z
INSDC status
public
Submitter Id
E-MTAB-9128:BM-3
age
8
broker name
ArrayExpress
cell type
neutrophil
common name
house mouse
developmental stage
adult
genotype
wild type genotype
organism part
bone marrow
sample name
E-MTAB-9128:BM-3
scientific_name
Mus musculus
sex
female
strain
BALB/cJ
tumor induction
4T1 cells were injected into the fourth mammary fat pad