Clematis terniflora
A RNA-Seq experiment includes 3 treatment groups, which are control (CT_1), high-level of UV-B radiation (1.21 w. m-2) (CT_2) and UV-B radiation followed by incubation in dark (CT_3), was conducted. The treatments are given as follows: 6-week-old C. terniflora plant leaves were radiated with UV-B for 5 hours followed by incubation in dark for 48 hours in a cabinet where temperature is controlled between 25-30 centigrade with 80% relative humidity. C. terniflora plant leave tissues before being radiated (control, CT_1), after 5 hours UV-B radiation (CT_2), and after 5 hours UV-B radiation followed by 48 hours incubation in dark (CT_3) were collected for total RNA extraction and RNA-seq sequencing, respectively. Total RNA was isolated by using RNeasy Plant Mini kit (Qiagen, Hilden, Germany). The total RNA was randomly fragmented (200 bp +- 25 bp) and reverse-transcribed into cDNA and then sequenced on the Solexa platform (Illumina HiSeq 2000) with 100 bp paired-end sequencing. As a result, 4 RNA-Seq datasets were generated, with one for CT_1, one for CT_2 and two for CT_3.
ecotype
Huzhou
age
6-weeks old
geo_loc_name
China: Zhejiang, Huzhou
tissue
leaf
BioSampleModel
Plant