Homo sapiens
Replicate 1 Protocols: iCLIP protocol was followed to isolate RNA bound to CPSF-160 protein and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction and are specified below for each sequencing run. Random barcodes were also incoperated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina pair-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
ENA-FIRST-PUBLIC
2014-10-01T17:02:25Z
ENA-LAST-UPDATE
2018-03-08T21:02:56Z
External Id
SAMEA2774593
INSDC center name
MRC Laboratory of Molecular Biology, Cambridge
INSDC first public
2014-10-01T17:02:25Z
INSDC last update
2018-03-08T21:02:56Z
INSDC status
public
Submitter Id
E-MTAB-2939:CPSF160_GFP repl1
broker name
ArrayExpress
cell line
HeLa
common name
human
sample name
E-MTAB-2939:CPSF160_GFP repl1
scientific_name
Homo sapiens