Thauera aminoaromatica
Thauera aminoaromatica MZ1T was originally isolated from an industrial wastewater treatment plant experiencing a prolonged episode of viscous bulking. We employ a Phenotype Sequencing approach in which mutations causing the phenotype can be identified directly from the sequencing of multiple independent mutant strains. In this study, flocculation mutants were created by chemical mutagenesis and screened for flocculation in vitro. The full genomes of two of these T. aminoaromatica strain MZ1T flocculation deficient mutants were sequenced using the Ion Torrent Personal Genome Machine, an integrated semiconductor-based genome-scale DNA sequencer. The millions of short reads generated were mapped to the reference genome of wild type T. aminoaromatica MZ1T.
strain
MZ1T
isolate
20A mutant
isolation_source
Wastewater treatment plant
collection_date
2004
geo_loc_name
USA:Tennessee
sample_type
cell culture
BioSampleModel
Microbe, viral or environmental