soil metagenome
Soil microbial biomass has been determined since the mid 1970s by the chloroform fumigation incubation technique as proposed by Jenkinson and Powlson (1976). The microbial biomass C can be determined by subtracting the CO2 emitted from an unfumigated soil (mineralization of soil organic matter) from that emitted from a chloroform fumigated inoculated soil (mineralization of soil organic matter and killed soil microorganisms) and dividing the difference by a mineralization factor (kC = 0.45). The question remained which microorganisms recolonized a fumigated soil. An arable soil was fumigated for one day with ethanol-free chloroform or left unfumigated and incubated aerobically after removal of the chloroform for 10 days. The bacterial population structures were determined in the fumigated and unfumigated soil after 0, 1, 5 and 10 days
env_package
MIGS/MIMS/MIMARKS.soil
investigation_type
metagenome
biome
Arid soil
collection_date
13-Jan-2016
feature
Chloroform Fumigated arable soil
geo_loc_name
Mexico: Estado de Mexico, Otumba
lat_lon
19.41 N 98.43 W
material
soil
depth
20 cm
elev
2349 masl
cur_land_use
small grains
annual_season_precpt
577 mm
annual_season_temp
14.8
ph_meth
CaCl2 0.1 M
ph
7.6
pool_dna_extracts
4
samp_weight_dna_ext
0.5 g
texture_meth
Bouyoucos hydrometer method
texture
84, 5, 11, Sandy loam
tot_n_meth
Kjeldahl
tot_n
0.8
tot_org_c_meth
Combustion and IR analysis
tot_org_carb
4.5
Day
0
Replicate
1
Treatment
Fumigated
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.soil