uncultured fungus
uncultured fungus from mineral fertilized soil 3
environment (material)
agricultural soil
collection date
2011-22-09
target gene
internal trancribed spacer
geographic location (longitude)
11.8798
history/tillage
disc plough
geographic location (elevation)
118
ENA-FIRST-PUBLIC
2014-03-03T14:25:28Z
ENA-LAST-UPDATE
2016-10-21T09:33:04Z
storage conditions (fresh/frozen/other)
-20 °C freezer, 6 months
pH
5.5
soil environmental package
soil
environment (biome)
cropland
environment (feature)
field
link to classification information
http://doi.org/10.1002/jpln.200521814
link to climate information
www.ufz.de/index.php?de=13170
history/previous land use
row crops rotation since 1902
amount or size of sample collected
500
adapters
CCATCTCATCCCTGCGTGTCTCCGAC, CCTATCCCCTGTGTGCCTTGGCAGT
mean annual and seasonal precipitation
540.3
organism
uncultured soil fungus
sample collection device or method
push core
current land use
row crops
links to additional analysis
www.ufz.de/index.php?de=16763
geographic location (latitude)
51.3895
scientific_name
uncultured soil fungus
geographic location (country and/or sea,region)
Germany
target subfragment
ITS1, 5.8S, ITS2 partial
investigation type
mimarks-survey
pcr primers
TCCTCCGCTTATTGATATGC, CTTGGTCATTTAGAGGAAGTAA
mean annual and seasonal temperature
9.8
horizon
A horizon
depth
0.1
project name
Multiple barcoding of soil microbial eukaryotes
sequencing method
pyrosequencing
history/agrochemical additions
150, 60, 120 and 80 kg . ha-1 nitrogen, 12, 0, 12 and 0 kg . ha-1 phosphate, 230, 0, 230 and 0 kg . ha-1 potassium for sugar beet, summer barley, potatoes and winter wheat respectively
history/crop rotation
8 years rotations ; potatoes, winter wheat, sugar beet, barley, potatoes, winter wheat, alfalfa, alfalfa ; 2004-2011
composite design/sieving (if any)
composite sample of ten cores pooled before sieving, 2 mm mesh size sieving
pH method
in H20
soil_taxonomic/FAO classification
Haplic Chernozem
multiplex identifiers
TAGTATCAGC
sample weight for DNA extraction
0.5
ENA-FIRST-PUBLIC
2014-03-03
ENA-LAST-UPDATE
2016-10-21