Homo sapiens
U2OS-TREx cells were cultured in DMEM supplemented with 10% fetal bovine serum (Tet Minus FBS; PanBiotech) and 1% penicillin/streptomycin (Invitrogen). Genomic DNA from pSpCas9(BB)-2A-Puro (PX459) V2.0 - empty vector transfected cells was extracted using Qiagen QiaAmp DNA mini kit (#51304) according to the manufacturers instructions and was used as a template for PCR amplification. Forward (5-CTGCTGGACAGAAAGGCAGT-3) and reverse (5-ATCCACAACGCTGAAGAGCGC-3) primers spanning the CRISPR-edited region  for amplification of a 450bp fragment of Exon2 of zfp36l1
isolate
15 year old female -unedited
age
15y
biomaterial_provider
Kalpana Surendranath, Genome Engineering Laboratory, 115 New Cavendish street, University of Westminster, London- W1W6UW, United Kingdom
sex
female
tissue
bone
cell_line
U2OS_T-REx
cell_subtype
Osteosarcoma
cell_type
Epithelial
dev_stage
Moderately differentiated sarcoma
disease
Human osteosarcoma
ethnicity
Caucasian
karyotype
Hypertriploid range
sample_type
Empty -pSpCas9(BB)-2A-Puro (PX459) V2.0 - transfected monoclonal cell line
treatment
None
BioSampleModel
Human