Populus tomentosa
isolate
leaf
cultivar
Populus tomentosa 741
dev_stage
exposed to light for 30 days
geo_loc_name
China: Beijing
tissue
calli from leaf
cell_line
WT_2_1
cell_type
wild type
collection_date
2019-01-08
growth_protocol
The subcultured cells in the dark condition were transferred to MS solid medium with 3% (w/v) Suc and 0.8 mg/L 2,4 D in total darkness at 25°C for 7 days. Subsequently, the cells were transferred to MS solid medium with 3% (w/v) Suc, 0.5 mg/L 6-BA and 0.5 mg/L KT, then exposed to light under the condition of a 14 h light/10 h dark photoperiod at 25°C for 30 days to induce callus.
treatment
cells exposed to light for 30 days
BioSampleModel
Plant