Saccharomyces cerevisiae
source_name
BY4741
strain
BY4741
growth and treatment
Cells were grown in YPD and synchronised in G1 by 2 hours treatment with 5ug/ml alpha-factor. Cells were released into S-phase by washing twice and resuspending in pre-warmed YPD, then harvested after 50 minutes
fix and post-processing
70% ethanol