uncultured alga
uncultured alga from mineral fertilized soil 3
ENA first public
2014-03-03
ENA last update
2016-10-21
External Id
SAMEA2397749
INSDC center alias
UFZ
INSDC center name
UFZ - Helmholtz Centre
INSDC first public
2014-03-03T14:25:28Z
INSDC last update
2016-10-21T09:33:04Z
INSDC status
public
Submitter Id
upaso13c
adapters
CCATCTCATCCCTGCGTGTCTCCGAC, CCTATCCCCTGTGTGCCTTGGCAGT
amount or size of sample collected
500
collection date
2011-09-22
composite design/sieving (if any)
composite sample of ten cores pooled before sieving, 2 mm mesh size sieving
current land use
row crops
depth
0.1
environment (biome)
cropland
environment (feature)
field
environment (material)
agricultural soil
geographic location (countryand/orsea,region)
Germany
geographic location (elevation)
118
geographic location (latitude)
51.3895
geographic location (longitude)
11.8798
history/agrochemical additions
150, 60, 120 and 80 kg . ha-1 nitrogen, 12, 0, 12 and 0 kg . ha-1 phosphate, 230, 0, 230 and 0 kg . ha-1 potassium for sugar beet, summer barley, potatoes and winter wheat respectively
history/crop rotation
8 years rotations ; potatoes, winter wheat, sugar beet, barley, potatoes, winter wheat, alfalfa, alfalfa ; 2004-2011
history/previous land use
row crops rotation since 1902
history/tillage
disc plough
horizon
A horizon
investigation type
mimarks-survey
link to classification information
http://doi.org/10.1002/jpln.200521814
link to climate information
www.ufz.de/index.php?de=13170
links to additional analysis
www.ufz.de/index.php?de=16763
mean annual and seasonal precipitation
540.3
mean annual and seasonal temperature
9.8
multiplex identifiers
TAGTATCAGC
pcr primers
GGACAGAAAGACCCTATGAA, TCAGCCTGTTATCCCTAGAG
ph
5.5
ph method
in H20
project name
Multiple barcoding of soil microbial eukaryotes
sample collection device or method
push core
sample name
upaso13c
sample weight for DNA extraction
0.5
sequencing method
pyrosequencing
soil environmental package
soil
soil taxonomic/fao classification
Haplic Chernozem
storage conditions (fresh/frozen/other)
-20 °C freezer, 6 months
target gene
universal plastid amplicon