Methylorubrum extorquens PA1
Wild-type populations of M. extorquens PA1 contain subpopulations of cells with especially high tolerance to formaldehyde. Upon addition of formaldehyde to the growth medium, the sensitive majority of cells loses viability, and only the tolerant cells continue to grow. We harvested a population of these tolerant cells after selection on 4 mM formaldehyde and re-sequenced the genome using Illumina HiSeq, in order to asses whether there were any genetic changes associated with high formaldehyde tolerance.
strain
CM2730
isolate
formaldehyde-tolerant subpopulation
isolation_source
selected via growth in liquid MPIPES-methanol medium with 4 mM formaldehyde
collection_date
2017-08-18
geo_loc_name
USA: Moscow, ID
sample_type
cell culture
biomaterial_provider
Marx Lab, University of Idaho
collected_by
Jessica Audrey Lee
env_biome
pure culture
samp_size
2 mL
temp
30 C
BioSampleModel
Microbe, viral or environmental