Tigriopus californicus
F2 hybrid clutches were hatched together in petri dishes, and individuals scored for date of metamorphosis into copepodid stage, and each frozen separately. After ~3000 individuals were scored, the 10% fastest were pooled for library prep and sequencing. Library was prepared using the Illumina Nextera kit, with gel size-selection in the range 350-500bp.
isolate
hybrid
isolation-source
intertidal pool
collection-date
2018-06
geo-loc-name
USA
tissue
whole animal
age
6 days
depth
intertidal
dev-stage
adult
sex
mixed
temp
20
experimental replicate
SDfxSHm F2 fast-growing
BioSampleModel
Invertebrate