Mus musculus
Protocols: Gonadal white adipose tissue was collected from adult male ReverbαFlox2-6AdipoCre+/-, Reverbα-/- global knockout (KO) or Reverbα wildtype mice (littermates of global knockouts) at ZT8, and flash frozen (n=6-8). Frozen tissue was homogenised in TRIzol Reagent (Invitrogen) using Lysing Matrix D tubes (MP Biomedicals) and total RNA extracted according to the manufacturer's TRIzol protocol as far as the isopropanol phase, at which point samples were transferred to columns from the ReliaPrep RNA Miniprep Tissue Kit (Promega) to ensure high quality RNA. The column was then washed, DNAse treated and RNA eluted as per Promega's protocol. Biological replicates were taken forward individually. Total RNA was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF). Quality and integrity of the RNA samples were assessed using a 2200 TapeStation (Agilent Technologies) and then libraries generated using the TruSeq® Stranded mRNA assay (Illumina) according to the manufacturer's protocol.
ENA-FIRST-PUBLIC
2021-03-01T12:15:59Z
ENA-LAST-UPDATE
2020-03-04T11:02:44Z
External Id
SAMEA6602595
INSDC center name
UNIVERSITY OF MANCHESTER
INSDC first public
2021-03-01T12:15:59Z
INSDC last update
2020-03-04T11:02:44Z
INSDC status
public
Submitter Id
E-MTAB-8840:Sample 43
age
28
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
diet
normal chow
genotype
ReverbFlox2-6AdipoCre+
individual
43
organism part
white adipose tissue
sample name
E-MTAB-8840:Sample 43
sampling site
gonadal fat pad
scientific_name
Mus musculus
sex
male
strain
C57BL/6