Mus musculus
Protocols: Fresh thalami isolated from adult mice were cut briefly and fixed with 1% formaldehyde for 10 minutes, chromatin isolated according to Cotney and Noonan, 2015 was sonicated into 200-500 bp fragments with Covaris S220 DNA was purified with Monarch PCR & DNA Cleanup Kit (T1030, NEB) Library preparation was conducted by NGS Core Facility, Centre of New Technologies, University of Warsaw. Libraries were prepared with KAPA HyperPrep Kit and KAPA Dual-Indexed adapters (Cat. no. 7962363001, KK8722 Kapa Biosciences). Enrichment by 15 cycles of amplification was applied and final library was size-selected using Kapa Pure Beads to obtain avarage size of 350 bp. Libraries were tested for quality with High Sensitivity DNA kit (Cat. no. 5067-4626, Agilent)
ENA-FIRST-PUBLIC
2020-07-24T04:03:48Z
ENA-LAST-UPDATE
2020-07-22T18:34:52Z
External Id
SAMEA7086101
INSDC center name
Laboratory of Molecular Neurobiology Centre of New Technologies University of Warsaw
INSDC first public
2020-07-24T04:03:48Z
INSDC last update
2020-07-22T18:34:52Z
INSDC status
public
Submitter Id
E-MTAB-9384:KO TCF7L2 1
age
60
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
TCF7L2 knockout
individual
15-20
organism part
thalamus
replicate
1
sample name
E-MTAB-9384:KO TCF7L2 1
scientific_name
Mus musculus
sex
male
strain
C57BL/6