Homo sapiens
Protocols: >1000-fold coverage of mutagenized cells (libraries A and B) was infected with either RV-C15 (MOI=1 PFU/cell) or EV-D68 Missouri (MOI=1 PFU/cell). RV-C15 infection was repeated for an additional round at 6 days post-infection. As soon as appearance of visibly viable colonies was observed, populations of virus-resistant cells were pooled and harvested. Uninfected starting populations of mutagenized cells were used as the unselected reference. Total genomic DNA from both virus-resistant and uninfected cells was respectively extracted using QIAamp DNA Mini Kit (Qiagen). The inserted guide RNA sequences were retrieved from the genomic DNA by PCR amplification.
ENA-FIRST-PUBLIC
2019-07-10T04:04:21Z
ENA-LAST-UPDATE
2019-07-08T16:07:34Z
External Id
SAMEA5762540
INSDC center name
Department of Microbiology & Immunology, Stanford University School of Medicine
INSDC first public
2019-07-10T04:04:21Z
INSDC last update
2019-07-08T16:07:34Z
INSDC status
public
Submitter Id
E-MTAB-8125:Sample 5
broker name
ArrayExpress
cell line
CDHR3 and Cas9 expressing H1-HeLa cells
common name
human
genetic modification
CRISPR knockout by GeCKO v2 library A (PMID: 25075903)
infect
RV-C15 infected
sample name
E-MTAB-8125:Sample 5
scientific_name
Homo sapiens