Mus musculus
Protocols: NS5 cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 NS5 cells were infected with pBABE-IRES-GFP-puro (NS5_control) or pBABE-Ascl1-ERT2-IRES-GFP-puro (NS5_Ascl1), followed by selection in complete medium containing 1ug/mL puromycin (Calbiochem), the latter sample collected 24 hours after induction with 4-Hydroxytamoxifen at a final concentration of 50nM, while reducing EGF concentration to 5ng/mL . DNAse-seq samples from NS5 cells were prepared as described by Song et al (Genome Res, 2011). Libraries were generated as in (Boyle et al., 2008; Song and Crawford, 2010) with a slight modification made to the linkers to increase ligation efficiency (Song et al., 2011, Genome Res).
ENA-FIRST-PUBLIC
2014-10-02T17:04:02Z
ENA-LAST-UPDATE
2018-03-08T17:09:20Z
External Id
SAMEA2340759
INSDC center name
Instituto Gulbenkian de Ciencia
INSDC first public
2014-10-02T17:04:02Z
INSDC last update
2018-03-08T17:09:20Z
INSDC status
public
Submitter Id
E-MTAB-2270:NS5_control
broker name
ArrayExpress
cell line
NS5
cell type
neuronal stem cell
common name
house mouse
genotype
pBABE-IRES-GFP-puro
sample name
E-MTAB-2270:NS5_control
scientific_name
Mus musculus