Oryza sativa Indica Group
Protocols: Oryza sativa L. Indica Pokkali seedlings were grown in growth chambers to the -3 leaf stage. At 2 weeks old, they were subjected to salt stress treatment by watering with 300 mM NaCl solution (or normal watering for control plants). Treatment was applied at 10:00 PST (2 hours post-dawn). Seedling shoots (stem and leaves) were sampled at 1 hour, 2 hours, 5 hours, 10 hours, and 24 hours post-treatment. Total cellular RNA was extracted using RNA Plant Reagent (Life Technologies) and treated with RNase-free Turbo DNase (Life Technologies) followed by a clean-up step using Plant RNeasy kits (Qiagen). Concentration, integrity and extent of contamination by ribosomal RNA were assessed using ND-1000 spectrophotometer (Thermo Fisher Scientific) and Bioanalyzer 2100 (Agilent Technologies). For details see Fox et al. (2009) Applications for Ultra-high-Throughput Sequencing. In: Belostotsky (ed.), Plant Systems Biology, vol. 553, pp. 79--108. Libraries were prepared using the TruSeq(TM) RNA Sample Preparation Kits (v2).
ENA first public
2016-04-15
ENA last update
2015-11-30
External Id
SAMEA2201316
INSDC center alias
OREGON STATE UNIVERS
INSDC center name
Oregon State University
INSDC first public
2016-04-15T17:01:39Z
INSDC last update
2015-11-30T20:33:51Z
INSDC status
public
Submitter Id
E-MTAB-1914:Pokkali_salt_1hr_rep3
broker name
ArrayExpress
common name
long-grained rice
cultivar
Pokkali
sample name
E-MTAB-1914:Pokkali_salt_1hr_rep3