Mus musculus
Protocols: Young Balb/c or C57Bl/6J SPF mice (10-12 weeks) were irradiated with 4 Gy and sacrificed 16 days after irradiation. Lymphocytes from peripheral lymph nodes and spleen were stained with TCRβ, CD8, CD44, CD49d, CD122 and a viability dye and sorted by BD Influx to Trizol LS. Each sample consisted of a pool of lymphocytes originating from 2-5 mice. Alternatively, young (7-12 weeks) and aged (12-19 months) Balb/c or C57Bl/6J mice were sacrificed and their lymphocytes were sorted following the same protocol. RNA was isolated using Trizol (Thermo Fisher Scientific) followed by in-column DNAse treatment using RNA clean&concentrator kit (Zymo Research) Libraries were prepared with a SMARTer® Stranded Total RNA-Seq - Pico Input Mammalian library preparation kit v2 (Takara).
ENA-FIRST-PUBLIC
2020-07-25T04:05:43Z
ENA-LAST-UPDATE
2020-05-19T16:50:58Z
External Id
SAMEA6848098
INSDC center name
Service Laboratory of Functional Genomics and Bioinformatics Institute of Molecular Genetics of the ASCR, v. v. i.
INSDC first public
2020-07-25T04:05:43Z
INSDC last update
2020-05-19T16:50:58Z
INSDC status
public
Submitter Id
E-MTAB-9091:Balb-Y-Naive_08
age
5 to 12
broker name
ArrayExpress
cell type
naive T cell
common name
house mouse
developmental stage
adult
genotype
wild type genotype
immunophenotype
CD44-, CD49d low
individual
6-8
organism part
mixed lymph node and spleen
sample name
E-MTAB-9091:Balb-Y-Naive_08
scientific_name
Mus musculus
sex
female
strain
BALB/cJ