Anas platyrhynchos
Duck hepatitis A virus 1 (DHAV-1) is a highly lethal virus that severely affected the duck industry worldwide. Long non-coding RNAs (lncRNAs) play nonnegligible roles in pathogen attack. However, the function of ducks lncRNAs during DHAV-1 insults remains unclear. Here, we conducted transcriptome sequencing to investigate the dynamic changes of host lncRNAs profiles in duck embryo fibroblasts (DEFs) infected with DHAV-1. Totally, 16589 lncRNAs were identified and genomic features were characterized. Additionally, 1908 and 1105 different expression lncRNAs (DELs) were screened at 12 and 24 hours post-infection (hpi). Moreover, we predicted the target genes to interact with DELs based on cis- and trans- regulatory manners and constructed lncRNA-target genes regulatory network. Finally, functional annotation analyses indicated putative target genes of DELs mainly participate in immune-related pathways, including the pattern recognition receptors (TLR3), signal transducer (STAT1), transcription factors, and effector molecular (IL6) and we confirmed the up-regulation of these target genes by qRT-PCR. Our study comprehensively analyzed the lncRNA profiles upon DHAV-1 infection and screened the target genes involved in innate immunity response, thereby revealing the essential roles of ducks lncRNAs and broadening our understanding of host-virus interactions.
strain
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isolate
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breed
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cultivar
Anas platyrhychos
ecotype
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age
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sex
not determined
tissue
duck embryo fibroblasts
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Model organism or animal