Homo sapiens
U2OS-TREx cells were cultured in DMEM supplemented with 10% fetal bovine serum (Tet Minus FBS; ) and 1% penicillin/streptomycin (Invitrogen). Genomic DNA from CRISPR/Cas9-targeted ZFP36L1 clones was extracted using Qiagen QiaAmp DNA mini kit (#51304) according to the manufacturers instructions and was used as a template for PCR amplification. Forward (5-CTGCTGGACAGAAAGGCAGT-3) and reverse (5-ATCCACAACGCTGAAGAGCGC-3) primers spanning the CRISPR-edited region were used for amplification of a 450bp fragment of Exon2 of zfp36l1
isolate
15 year old female -edited on Chromosome 14 location 14q24.1
age
15y
biomaterial_provider
Kalpana Surendranath, Genome Engineering Laboratory, 115 New Cavendish street, University of Westminster, London- W1W6UW, United Kingdom
sex
female
tissue
bone
cell_line
U2OS_T-REx
cell_subtype
Osteosarcoma
cell_type
Epithelial
dev_stage
Moderately differentiated sarcoma
disease
Human osteosarcoma
ethnicity
Caucasian
karyotype
Hypertriploid range
sample_type
CRISPR/CAS9 edited monoclonal cell line
treatment
None
BioSampleModel
Human