Arabidopsis thaliana
Protocols: 17-day-old (DAS) shoot tissues of plants were harvested. Shoot tissues, consist of stems including the leaves, were harvested and immediately stored in liquid nitrogen. Seedlings were grown on half strength (1/2) MS medium containing 1% sucrose at 21˚C under a 16 h/8 h day/night condition. RNA samples for small RNA sequencing analyses were extracted from 17-day-old shoot tissues of plants by the miRNeasy Mini Kit (Qiagen). Small RNAs (<50 nt) were gel-purified to build the libraries with TruSeq small RNA sample prep kit (illumina).
ENA-FIRST-PUBLIC
2019-05-03T04:03:17Z
ENA-LAST-UPDATE
2019-04-23T13:24:54Z
External Id
SAMEA5574670
INSDC center name
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology, VIB, Gent, Belgium
INSDC first public
2019-05-03T04:03:17Z
INSDC last update
2019-04-23T13:24:54Z
INSDC status
public
Submitter Id
E-MTAB-7874:sgi6_R2
age
17
broker name
ArrayExpress
common name
thale cress
developmental stage
1 main shoot growth stage
genotype
EMS mutation of RST1 in ERF6-GR background
organism part
shoot
replicate
biological replicate
sample name
E-MTAB-7874:sgi6_R2
scientific_name
Arabidopsis thaliana
ecotype
Col-0