Homo sapiens
Protocols: H&E-stained slides of all FFPE blocks were assessed for tumor cell content, and the most suitable FFPE block was selected for DNA extraction. For each patient, six tissue shavings per FFPE block, cut at a thickness of 10 µm each, were submitted for RNA extraction. In cases in which the tumor purity in the selected FFPE block was lower than 70%, vital tumor was enriched by macrodissection, scratching unstained slides after the selection of cellular areas by H&E assessment. Total RNA from FFPE macrodissected tissues was extracted by the RNeasy FFPE kit (Qiagen) according to manufacturer's instructions on a Qiacube machine (Qiagen). Sequencing libraries were constructed starting from 50-100ng of total RNA by optimizations of the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen GmbH).
ENA-FIRST-PUBLIC
2020-09-29T17:07:48Z
ENA-LAST-UPDATE
2020-09-25T17:49:41Z
External Id
SAMEA7340267
INSDC center name
Department of Experimental Oncology, IEO, European Institute of Oncology IRCSS, Milan, Italy
INSDC first public
2020-09-29T17:07:48Z
INSDC last update
2020-09-25T17:49:41Z
INSDC status
public
Submitter Id
E-MTAB-9605:FFPE-HGSOC-143
broker name
ArrayExpress
cell type
Macrodissected solid tumor
common name
human
disease
high grade ovarian serous adenocarcinoma
sample name
E-MTAB-9605:FFPE-HGSOC-143
scientific_name
Homo sapiens
sex
female
specimen with known storage state
paraffin specimen