Populus tremula x Populus alba
Protocols: Leaves were cut off and directly frozen in liquid nitrogen Populus x canescens plants were propagated and cultivated as described by Müller et al. (2013). Plants were in vitro multiplicated by micro cuttings and grown on half strength Murashige & Skoog medium for three weeks at 23 °C/24 °C, 60-85 μE×m−2×s−1 photosynthetically active radiation (PAR) and a 16 h/8 h day/night regime. Subsequently, the plants were transferred in an in vitro sandwich system (Müller et al. 2013) and either cultivated in the presence or absence of the ectomycorrhizal fungus Laccaria bicolor. After three weeks, the plants were potted into 0.65 L pots containing a mixture of washed and twice autoclaved sand (0.71-1.21 mm and 0.4-0.8 mm particle size and soil a ratio of 10:2 v/v). For acclimation the plants were covered with a transparent plastic beaker for one week, which got gradually lifted during the second week and ultimately removed after 14 days. All plants were treated with modified Long Ashton nutrient solution (Hewitt, 1952) containing 2 mM KNO3 excess nutrient solution ran off. The plants got transferred into 3 L pots containing the same substrate after 4 weeks of greenhouse cultivation. Plants were randomized regularly. The leaves were harvested for transcriptome analysis after 13 weeks of green house cultivation. Plants were cultivated in the presence or absence of the ectomycorrhizal fungus Laccaria bicolor (see growth protocol). RNA from Populus leaves was extracted using the innuPrep Plant RNA kit (Analytik Jena, Germany) according to the manufacturer's protocol Libraries were constructed using the TrueSeq RNA Library Prep Kit (Illumina) according to the manufacturer's instructions
ENA-FIRST-PUBLIC
2021-03-01T12:16:10Z
ENA-LAST-UPDATE
2020-04-13T13:01:28Z
External Id
SAMEA6663813
INSDC center name
Georg-August-Universitaet Buesgen-Institut Department of Forest Botany and Tree Physiology
INSDC first public
2021-03-01T12:16:10Z
INSDC last update
2020-04-13T13:01:28Z
INSDC status
public
Submitter Id
E-MTAB-8929:Sample 6
age
19
broker name
ArrayExpress
cultivar
INRA 717-1B4
genotype
wild type genotype
growth condition
co-cultivated (Laccaria bicolor)
organism part
leaf
sample name
E-MTAB-8929:Sample 6
scientific_name
Populus tremula x Populus alba