Homo sapiens
Protocols: Human bronchial epithelial cells BEAS-2B were maintained in LHC-9 medium at 37°C and 5% of CO2 Cells for treatments were seeded at a concentration of 2.7x105 cells/well in Cell BIND 6-well plates (Corning), and treated the day after with diesel or biomass UFP. Cells were sampled at 1, 4, 8, 16, and 20 hours after a single exposure to 2.5 μg/cm2 (25 μg/ml) of UFP and compared to untreated controls at the same time of exposure. BEAS-2B cells exposed to 2.5 μg/cm2of UFP or medium alone were collected at 1, 4, 8, 16, and 20 hours, lysed, and stored in QIAzol Lysis reagent (Qiagen, Hilden, Germany) until RNA extraction. Three independent biological replicates were examined at each time point in UFP exposed and paired controls samples for a total of 45 samples. Total RNA was extracted using the miRNeasy extraction kit (Qiagen, Hilden, Germany) and eluted in RNase free-water, according to the manufacturer's recommended guidelines. Quality and quantity of the total RNA samples were evaluated with 2100 Bioanalyzer (Agilent, Santa Clara, CA) and Nanodrop 1000 (Thermo Fisher Scientific, Wilmington, DE), respectively. RNA TruSeq Stranded mRNA Library Prep Kit in paired end format (Illumina) starting from 1.5 ug of total RNA)
ENA first public
2018-05-09
ENA last update
2016-10-17
External Id
SAMEA4514706
INSDC center alias
Department of medical biotechnology and translational medicine (BIOMETRA) University of Milan
INSDC center name
Department of medical biotechnology and translational medicine (BIOMETRA) University of Milan
INSDC first public
2018-05-09T17:02:39Z
INSDC last update
2016-10-17T11:41:55Z
INSDC status
public
Submitter Id
E-MTAB-5157:h7
broker name
ArrayExpress
cell line
BEAS-2B
cell type
respiratory epithelial cell
common name
human
sample name
E-MTAB-5157:h7