EG1 p300i Rep B ChIP
Mus musculus
source_name
iPSCs
cell type
iPSCs reprogrammed from MEFs
strain
R26Fucci2aR (mixed C57BL/6, DBA/2, and CD1 background)
treatment
Early G1 cells were collected after: 6hr treatment with Nocodazole (200ng/ml) & 3hr simultaneous treatment with p300i (20uM A485), then mitotic shake off. Mitotic cells were released into fresh media for 1hr to enter early G1.
chip antibody
H3K27ac (Abcam, ab4729)