filtrate_BALB/c_002
mouse gut metagenome
host
Mus musculus
isolation_source
fecal bacteria
collection_date
2017-02-06
geo_loc_name
Germany: Berlin
lat_lon
52.52 N 13.37 E
samp_mat_process
collection, filtration through 0.45 micrometer filter, DNA extraction
bio_sample_titel
Fecal bacterial sample BALB/c mouse
biological_replicate
2
library_consrturction
Fresh feces from BALB/c mice were collected and resuspended in ice-cold PBS. The fecal suspension was subsequently filtered through a 70 micrometer (Corning), 30 micrometer (Partec, Grlitz) cell strainer, 5 micrometer (Merck via Sigma, Darmstadt) and 0.45 micrometer (Sarstedt, Nmbrecht) bacterial syringe filters. After centrifugation the pellets were incubated for 45 at 95C with 200 microliter of bidistelled water, the solution containing the DNA was frozen at -70C until further usage. The deep sequencing analysis was performed by LGC Genomics, Berlin, Germany using Illumina MiSeq V3 Chemistry (300 bp paired-end read) and Bacteria 16S (341F-785R) amplicon.
BioSampleModel
Metagenome or environmental