seawater metagenome
investigation_type
metagenome
project_name
Near-Coral and Near-Seagrass Water Metagenomes from Dry Tortugas National Park
collection_date
2017-06-18
env_package
mims extension
env_biome
ocean
env_feature
adjacent to coral
env_material
water
geo_loc_name
USA: Dry Tortugas National Park
lat_lon
24.70256 N 82.79935 W
depth
3.7-4.6 m
samp_collect_device
Peristaltic pump
samp_mat_process
Water (5L) was pumped through 0.8 um prefilters and 0.2 um Sterivex filters. Sterivex filters were then frozen in liquid nitrogen until DNA extraction within 14 days.
nucl_acid_ext
Manufacturer's protocol for MO BIO PowerWater Sterivex DNA Isolation Kit; modified protocol to eliminate Steps 8 and 9 (heating and cooling steps)
lib_const_meth
Nextera DNA Library Prep Kit
seq_meth
Illumina HiSeq 4000 generating paired-end 150-bp reads
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.water