SKOV3ip1_NOP58KD_1
Homo sapiens
source_name
SKOV3ip1 cell line
cell line
SKOV3ip1
cell type
ovarian adenocarcinoma
growth protocol
grown in DMEM/F12 (50/50) medium (Wisent), supplemented with 10 % fetal bovine serum and 2 mM L-glutamine, cells passaged as recommended by ATCC
treatment
Treated with siRNA targeting NOP58 (CUUCUACCGUUCAGAUUCU). Transfection was performed in suspension using Lipofectamine 2000 (Life technologies) according to the manufacturer’s protocol and 20 nM of siRNA (Sigma) for 48 hours.