Mit p300i Rep B ChIP
Mus musculus
source_name
iPSCs
cell type
iPSCs reprogrammed from MEFs
strain
R26Fucci2aR (mixed C57BL/6, DBA/2, and CD1 background)
treatment
Mitotic cells were collected after: 6hr treatment with Nocodazole (200ng/ml) then mitotic shake-off. Cells were also treated with p300i (20uM A485) for 3hrs prior to mitotic shake-off.
chip antibody
H3K27ac (Abcam, ab4729)