iCdx2_ES_MEF
Mus musculus
source_name
Embryonic Stem Cells
cell type
Embryonic Stem Cells
cell line
5ECER4G20
condition
5 days of reprogramming in TS conditions on MEFs, and then replated for a further 3 days on MEFs
treatment protocol
During the experimental time course of trans-differentiation, cells were plated on MEFs in TS cell conditions, as specified, with 4HT at a seeding denisty of approximately 1x10E4 cells/25cm2. Media was changed every 2 days. After 5 days, cells were trypsinised and replated with and without MEFs for a further 3 days culture. At the end of the trans-differentiation time-course, cells were FACS-purified to remove MEFs.