Sulfate-stimulated peat soil microcosm, amended with acetate, incubated for 5 days (replicate 1)
peat metagenome
collection_date
2010-09-21
depth
0.1-0.2 m
elev
713 m
env_biome
fen
env_feature
long-term experimental site
env_material
peat soil
geo_loc_name
Germany: Schloeppnerbrunnen fen II, Fichtel Mountains, northeastern Bavaria
lat_lon
50.131881 N 11.881046 E
ph
4.1-5.0
ref_biomaterial
27015005
rel_to_oxygen
anaerobe
samp_mat_process
Excerpt from PMID 27015005: Peat soil microcosms were set up by mixing 30 g of fresh soil (10-20 cm depth) with 60 ml of filter-sterilized (0.2 μm) and anoxic peat water in 250 ml sterile glass bottles. Microcosms were sealed under 100 % N₂ with butyl rubber septa and incubated for 53 days in the dark at 14 °C. Microcosms were periodically amended from the first day of incubation with formate, acetate, propionate, lactate, or butyrate, or incubated without any external substrate. Half of the substrate-amended and -unamended microcosms additionally received periodic amendments of sulfate. These microcosms were initially spiked with sulfate, and, thereafter, were periodically amended with small amounts of sulfate. Triplicate microcosms were set up per incubation condition (36 microcosms in total).
samp_size
30 g
samp_vol_we_dna_ext
0.3 g
source_material_id
Sb_2010.microcosm_07.day_05
water_content_soil
0.78 g/g
sulfate_stimulated
yes
amended_substrate
acetate
incubation_time_days
5
microcosm_id
7
replicate
1
BioSampleModel
MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.soil