Solanum pennellii
cultivar
LA0716
age
7 weeks
geo_loc_name
Germany
tissue
leaf
biomaterial_provider
Anika Wiese-Klinkenberg, FZ Jlich, Germany
collected_by
Laura Junker
culture_collection
none
genotype
Solanum lycopersicum
growth_protocol
Seeds underwent stratification for at least 5 days before sowing. Plants were grown from seeds inenvironmental chambers (Hhren Klte-Klima-Elektrotechnik, Erkelenz, Germany), equipped withmetal halide lamps (Philips, Hamburg, Germany) undercontrolled conditions of 22/18 C during day and night, a relative humidity of 50% and 200 mol m-2s-1 photons light intensity for 10 h per day.Plants were sowed in rock wool plugs (2x2x4 cm; Grodan, Roermond, The Netherlands), thatwere four times prewashed with deionized water. Seeds were placed around 0.5 cm below the surface of the rock wool plugs. A total number of 150 seeds per species were seeded. The plugs were watered with water for 16 days. 84 seedlings, which already developed the first real leaf, were then transferred to rock wool blocks (7.5x7.5x6.5 cm) (Grodan, Roermond, The Netherlands), that were three times prewashed with deionized water. Afterwards, the seedlings were fertilized with half-strength Hoagland solution for 14 days, followed by full-strength Hoagland solution (5mM KNO3, 5mM Ca(NO3)2, 2mM MgSO4, 1mM KH2PO4, 90 M FeEDTA, plus micronutrients) for further 11 days.
sample_type
4. true leaf
temp
22/18
treatment
nitrogen defiecency, reduced or elevated temperature, elevated light conditions
BioSampleModel
Plant