Plant sample from Capsicum annuum var. Mazurka
Capsicum annuum
cultivar
Mazurka
age
10 weeks
geo_loc_name
Germany
tissue
leaf
biomaterial_provider
Anika Wiese-Klinkenberg, FZ Juelich, Germany
collected_by
Franziska Genzel
disease
none
growth_protocol
Seeds underwent stratification for at least 5 days before sowing. Plants were grown from seeds in environmental chambers (Huehren Kaelte-Klima-Elektrotechnik, Erkelenz, Germany), equipped with metal halide lamps (Philips, Hamburg, Germany), under controlled conditions of 24/18 during day and night, a relative humidity of 65 percent and 200 mikro mol /m2 s photons light intensity for 10 h per day. Plants were sowed in rock wool plugs (2x2x4 cm; Grodan, Roermond, The Netherlands), that were four times prewashed with deionized water. Seeds were placed around 0.5 cm below the surface of the rock wool plugs. A total number of 100 seeds per species were seeded. The plugs were watered with water for 14 days. 54 seedlings, which already developed the first real leaf, were then transferred to rock wool blocks (7.5x7.5x6.5 cm) (Grodan, Roermond, The Netherlands), that were three times prewashed with deionized water. Afterwards, the seedlings were fertilized with half-strength Hoagland solution for 14 days, followed by full-strength Hoagland solution (5 mM KNO3, 5 mM Ca(NO3)2, 2 mM MgSO4, 1 mM KH2PO4, 90 mikro M FeEDTA, plus micronutrients) afterwards.
sample_type
6. true leaf
temp
24/18
treatment
cooler temperatures of 20/12, or 200 mM NaCl for up to two weeks
BioSampleModel
Plant