Medicago truncatula
Title
Mtrun-A17-026-G1
Genotype
jemalong A17
Molecule
genomic_DNA
Extract_protocol
DNA was extracted as describ (Mayjonade et al 2016) from Medicago truncatula A17 leaves. The following steps were performed using the Get-PlaGe core facility (INRA, Toulouse, France). Seven libraries were produced, using the SMRTbell Template Prep Kit 1.0, each from 7.5 µg DNA fragmented with a Megaruptor system (Diagenode, RRID: SCR_014807). The DNA was repaired (DNA damage Repair and END repair kits) then ligated to hairpin adapters. Following digestion of incompletely formed SMRTbell templates with Exonuclease III and VII, DNA molecules between 17 and 90 kb were selected by BluePippin electrophoresis (Sage Science, RRID:SCR_014808). 