Homo sapiens
source_name
T-cells
tissue
T-cells
subject id
I
diagnosis
healthy control
treatment
Ox mtDNA
activation
Ox mtDNA generation and pDC activation: Ox mtDNA was generated as previously described. Briefly, healthy neutrophils were pre-incubated with IFNα2β (2000 U/ml; Schering Corp.) for 90 min at 370C and then extensively washed before incubation with anti-RNP IgG (50 μg/ml) purified from SLE patient sera. Neutrophil supernatants were then collected, centrifuged for 10 min at 1400g and stored at -80C. PDCs (5x105 cells/well – 96 U bottom plate) were cultured with 40% v/v Ox mtDNA-containing neutrophil supernatants (referred in the text as “Ox mtDNA”) or with 5 μg/ml of either CpGA (ODN-2216; Invivogen) for 24 h. The volume of Ox mtDNA-containing neutrophil supernatants and the concentration of CpGA were selected based on their capacity to trigger similar levels of IFNα production by pDCs.
project
1