Oryza sativa Japonica Group
cultivar
Nipponbare
biomaterial_provider
Kieber Lab, 4163 Genome Sciences Building, Chapel Hill, NC
tissue
shoots
age
10 days
growth_protocol
Rice seeds (Nipponbare) were surface sterilized with 2.5 percnet sodium hypochlorite for 15 minutes and germinated on filter paper containing distilled water at 37 degrees C for one day. Germinated seeds were transferred to hydroponic culture containing Kimura B solution in a growth chamber set to 14 hours light (28C) and 8 hours of dark (23C) with light intensity 700 micro-mol /m2s. Plants were grown in two pots. At day ten of hydroponic cultivation, culture media was replaced with new media containing 5 microMolar BA or 0.05 mN NaOH as a control. Following 120 minutes of the treatment, roots and shoots were harvested separately. Roots and shoots from different pots were pooled to form three replicates.
treatment
control hydoponic media, containing no 6-benzylaminopurine
geo_loc_name
missing
BioSampleModel
Plant