Mus musculus
Protocols: Genital ridges were dissected away from the mesonephros in sterile PBS and stored at -80 °C until all samples were collected. Three pairs of gonads from different animals were grouped to produce each sample. RNA was extracted using a Direct-zolTM RNA MiniPrep Kit (Zymo Research, CA, USA) following the manufacturer's protocol. DNA digestion was performed with RQ1 RNase-Free DNase (Promega, WI, USA) [60]. Total RNA was eluted in 50 µl RNase-free water. Immediately after extraction, total RNA was cooled to -80 ºC until further use. RNA-seq libraries were prepared from three grouped gonads from three E11 and E12 males and females.
ENA-FIRST-PUBLIC
2019-02-21T17:05:12Z
ENA-LAST-UPDATE
2019-02-11T13:03:46Z
External Id
SAMEA5331412
INSDC center name
1.Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Madrid, Spain. 2. School of Agriculture and Food Science, University College Dublin, Dublin, Ireland.
INSDC first public
2019-02-21T17:05:12Z
INSDC last update
2019-02-11T13:03:46Z
INSDC status
public
Submitter Id
E-MTAB-7656:F12-2
age
Fetus
broker name
ArrayExpress
common name
house mouse
developmental stage
E12
genotype
wild type genotype
individual
24480
organism part
embryonic gonadal ridge
sample name
E-MTAB-7656:F12-2
scientific_name
Mus musculus
sex
female
strain
cd1