Leishmania mexicana
PRO2, cultured promastigotes in exponential growth phase Protocols: Promastigote-form L. mexicana (WHO strain MNYC/BZ/62/M379) were grown at 28°C in M199 medium (Life Technologies) supplemented with 2.2 g/L NaHCO3, 0.005% haemin, 40 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) pH 7.4 and 10% FCS (Life Technologies 10500-064). Cells were maintained in exponential growth phase prior to RNA extraction. For mapping of splice acceptor sites and determination of transcript abundances, polyadenylated RNA was purified from total RNA, converted to cDNA using random hexamer primers, sheared and size selected for fragments ~200bp in length using the Illumina TruSeq RNA Sample Preparation Kit v2.
ENA first public
2015-09-11
ENA last update
2015-03-16
External Id
SAMEA3306028
INSDC center alias
Sir William Dunn School of Pathology University of Oxford
INSDC center name
Sir William Dunn School of Pathology University of Oxford
INSDC first public
2015-09-11T17:01:24Z
INSDC last update
2015-03-16T14:45:16Z
INSDC status
public
Submitter Id
E-MTAB-3312:Sample 9
broker name
ArrayExpress
developmental stage
promastigote
host
not applicable (outside host)
sample name
E-MTAB-3312:Sample 9