marine sediment metagenome
RNA-Stable isotope  probing incubation = SIP incubation
isolation_source
marine sediment
collection_date
Not applicable
geo_loc_name
South Georgia and the South Sandwich Islands: South Georgia, Cumberland Bay
lat_lon
54.265 S 36.437 W
rel_to_oxygen
obligate anaerobe
samp_collect_device
anoxic sampling of sediment slurry incubations
samp_mat_process
Previously collected sediments from South Georgia, Cumberland Bay GeoB 22024-1 (0-14 cm core depth) were stored at 4°C (near in-situ temperature) until use as starting material for incubation experiments. For RNA-SIP incubations, anaerobic slurries were prepared in ratio 1:4 with 13C labelled or unlabelled (12C) acetate. Sample name indentifies substrate and SIP fraction
samp_size
cDNA obtained from RNA after ultracentrifugation with 1 µg RNA
source_material_id
light fraction RNA from SIP incubation with un-labelled acetate + lepidocrocite + molybdate sequenced with bacterial 16S rRNA primers
BioSampleModel
Metagenome or environmental