rice paddy metagenome
An enrichment medium containing a variety of electron acceptors (EA medium), was used to develop an anaerobic enrichment from a Vietnamese rice paddy soil. The EA medium consisted of 6 mM NaNO3, 6 mM Na2SO4, 5 mM ferric citrate, 0.01% yeast extract, 35 mM cellobiose, and 1/4 strength TSB (tryptic soy broth). This BioSample corresponds to the EA enrichment grown without arsenic, spiked  with 25 micromolar arsenite after 7.5h and sampled 30 min after arsenic spike (8h)
isolation_source
Vietnamese rice paddy soil
collection_date
2014-05
geo_loc_name
Viet Nam: An Giang Province, An Phu District
lat_lon
10.905 N 105.079 E
ref_biomaterial
primary publication
rel_to_oxygen
anaerobe
samp_mat_process
5 g of wet rice paddy soil were incubated in 10 mL of EA medium, 1 mL of the culture was transferred into 10 mL of new, sterile growth EA medium. This procedure was then repeated three times to obtain the EA anaerobic rice paddy soil-derived microbiome. In this BioSample, the enrichment was grown without arsenic, spiked after 7.5h with 25 micromolar arsenite and sampled 30 min after spike.
BioSampleModel
Metagenome or environmental