Mus musculus
Protocols: Cells were harvested by trypsinization To differentiate naïve ESCs into Epiblast-like cells (EpiLCs), flasks were first pre-treated with Geltrex (Life Technologies) diluted 1:100 in DMEM/F12 (Life Technologies) and incubated at 37 °C overnight. Naïve ESCs were plated on Geltrex-treated flasks in defined medium containing 10 ng/mL Fgf2 (R&D Systems), 20 ng/mL Activin A (R&D Systems) and 0.1× Knockout Serum Replacement (KSR) (Life Technologies). Media was changed after 24 h and EpiLCs were harvested for RRBS and RNA-seq experiments after 48 h. column purification SCRB-seq adapted for bulk samples (Ziegenhain, C., Vieth, B., Parekh, S., Reinius, B., Guillaumet-Adkins, A., Smets, M., Leonhardt, H., Heyn, H., Hellmann, I., Enard, W., 2017. Comparative Analysis of Single-Cell RNA Sequencing Methods. Mol. Cell 65, 631-643.e4.)
ENA-FIRST-PUBLIC
2020-06-29T04:03:40Z
ENA-LAST-UPDATE
2019-10-21T16:29:53Z
External Id
SAMEA6104706
INSDC center name
Faculty of Biology Department II Ludwig Maximilians University Munich
INSDC first public
2020-06-29T04:03:40Z
INSDC last update
2019-10-21T16:29:53Z
INSDC status
public
Submitter Id
E-MTAB-6797:wt_EpilC_rep4
broker name
ArrayExpress
cell line
J1
cell type
epiblast like cell
common name
house mouse
developmental stage
blastocyst
genotype
wild type genotype
organism part
inner cell mass
sample name
E-MTAB-6797:wt_EpilC_rep4
scientific_name
Mus musculus
strain
129S4/SvJae