rice paddy metagenome
An enrichment medium containing a variety of electron acceptors (EA medium), was used to develop an anaerobic enrichment from a Vietnamese rice paddy soil. The EA medium consisted of 6 mM NaNO3, 6 mM Na2SO4, 5 mM ferric citrate, 0.01% yeast extract, 35 mM cellobiose, and 1/4 strength TSB (tryptic soy broth). This BioSample corresponds to the EA enrichment grown without arsenic and sampled 46h after inoculation.
isolation_source
Vietnamese rice paddy soil
collection_date
2014-05
geo_loc_name
Viet Nam: An Giang Province, An Phu District
lat_lon
10.905 N 105.079 E
ref_biomaterial
primary publication
rel_to_oxygen
anaerobe
samp_mat_process
5 g of wet rice paddy soil were incubated in 10 mL of EA medium, 1 mL of the culture was transferred into 10 mL of new, sterile growth EA medium. This procedure was then repeated three times to obtain the EA anaerobic rice paddy soil-derived microbiome. In this BioSample, the sample was taken 46h after inoculation.
BioSampleModel
Metagenome or environmental