Mus musculus
Protocols: At day 10 post treatment, the mice were euthanized and Ly6G+ neutrophils were isolated from lung by using anti-Ly6G MicroBead Kit (Miltenyi Biotec) following manufacturer's instructions. The isolated neutrophils were analyzed by flow cytometry and the cells with a > 95% purity were used for the next procedure. AT3-g-csf cells (5x10^5) were injected into the fourth mammary fat pads of female WT and Atgl-cKO mice (10-week-old, n = 4/group). Total RNA was isolated from neutrophils using the miRNeasy Mini kit (Qiagen). Stranded libraries were prepared using the KAPA mRNA HyperPrep Kit (KAPA Biosystems): isolation of polyA containing mRNA using oligo-dT magnetic beads, RNA fragmentation, first and second strand cDNA synthesis, ligation of Illumina-specific adapters containing a unique barcode sequence for each library, and PCR amplification. Libraries were checked for quality and concentration using the DNA 1000 assay (Agilent Technologies) and quantitative PCR (KAPA Biosystems), according to the manufacturers' instructions.
ENA-FIRST-PUBLIC
2020-06-28T04:03:54Z
ENA-LAST-UPDATE
2020-06-18T23:28:00Z
External Id
SAMEA6990730
INSDC center name
THE JACKSON LABORATORY
INSDC first public
2020-06-28T04:03:54Z
INSDC last update
2020-06-18T23:28:00Z
INSDC status
public
Submitter Id
E-MTAB-9235:ATGL-2
age
12
broker name
ArrayExpress
cell type
neutrophil
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
ATGL-2
organism part
lung
sample name
E-MTAB-9235:ATGL-2
scientific_name
Mus musculus
sex
female
strain
B6