Mus musculus
Protocols: Male and female Reverb alpha global knockout mice and their wild-type littermate controls, aged between 9-16 weeks, were housed on a 12hr:12hr light:dark cycle, with ad libitum access to food and water. Mice were injected at ZT6 or ZT18 with 1mg/kg dexamethasone (Sigma) i.p.. One hour later, mice were culled by cervical dislocation, and livers harvested and flash frozen. ChIP was performed using the ChIP-IT High Sensitivity kit (Active Motif) according to manufacturer instructions. A TissueRuptor (Qiagen) was used for 45s at maximum speed to disrupt liver tissue, followed by dounce homogenising for 70 strokes to release cell nuclei. Nuclear sonication was conducted with the EpiShear probe sonicator (Active Motif, 3.5mm probe) for 8 rounds of 2 minutes per sample, at 37% amplitude. 15ug sheared chromatin was incubated with anti-H3K27Ac antibody (Active Motif, Lot No: 31814008). Antibody was pulled down with magnetic protein G agarose microspheres (ReSyn Biosciences). After elution and de-crosslinking ChIP DNA was purified as per ChIP-IT High Sensitivity kit instructions. 5-10ng ChIP DNA was submitted to the University of Manchester Genomics Facility for library preparation and paired-end sequencing on the Illumina HiSeq 4000 platform.
ENA-FIRST-PUBLIC
2018-11-13T17:03:37Z
ENA-LAST-UPDATE
2018-07-10T17:20:58Z
External Id
SAMEA4784825
INSDC center name
UNIVERSITY OF MANCHESTER
INSDC first public
2018-11-13T17:03:37Z
INSDC last update
2018-07-10T17:20:58Z
INSDC status
public
Submitter Id
E-MTAB-6994:Input_KO_Day_Rep2
age
9 to 16
broker name
ArrayExpress
common name
house mouse
compound
dexamethasone
dose
1
genotype
Nr1d1 homozygous knockout
organism part
liver
sample name
E-MTAB-6994:Input_KO_Day_Rep2
scientific_name
Mus musculus
sex
male
strain
C57BL/6J