Leishmania mexicana
PRO2, cultured promastigotes in exponential growth phase; used to generate library enriched for transcript 3'ends Protocols: Promastigote-form L. mexicana (WHO strain MNYC/BZ/62/M379) were grown at 28°C in M199 medium (Life Technologies) supplemented with 2.2 g/L NaHCO3, 0.005% haemin, 40 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) pH 7.4 and 10% FCS (Life Technologies 10500-064). Cells were maintained in exponential growth phase prior to RNA extraction. For determination of polyadenylation addition sites, polyadenylated RNA purified from total RNA was converted to cDNA using a 5'-T15VN-3' oligonucleotide (V=A, G or C; N=T, A, G or C) primer to enrich for 3' ends of transcripts. The cDNA was sheared and size selected for fragments ~200bp in length using the Illumina TruSeq RNA Sample Preparation Kit v2.
ENA first public
2015-09-11
ENA last update
2015-03-16
External Id
SAMEA3317387
INSDC center alias
Sir William Dunn School of Pathology University of Oxford
INSDC center name
Sir William Dunn School of Pathology University of Oxford
INSDC first public
2015-09-11T17:01:24Z
INSDC last update
2015-03-16T14:45:15Z
INSDC status
public
Submitter Id
E-MTAB-3312:Sample 15
broker name
ArrayExpress
developmental stage
promastigote
host
not applicable (outside host)
sample name
E-MTAB-3312:Sample 15