freshwater metagenome
This dataset contains raw 16S amplicon Illumina MiSeq reads collected with Biological OsmoSamplers deployed the Department of Energy's Biological and Environmental Research Station (DOE BER) at East River in Crested Butte, Colorado, USA. The samples represent the two sections of Biological OsmoSampler sample coil, both approximately 7 m in length, containing roughly 10 mL of river water each. This research was preformed as part of the SBR funded project "Quantifying subsurface biogeochemical variability in a high altitude watershed during winter isolation" DE-SC0018328. The novel part of this dataset is that we identify the riverine microbiome throughout the winter isolation period, which is difficult to measure.
isolation_source
Water column of East River, CO
collection_date
2018-09
geo_loc_name
USA: U.S. Department of Energy's Biological and Environmental Research (DOE BER) station located at East River (ER) in Crested Butte, CO
lat_lon
38.922 N 106.951 W
ref_biomaterial
Buser-Young, J, et al., Hidden processes during seasonal isolation of a high-altitude watershed. Submitted to Frontiers in Earth Sciences, Feb 2021
samp_collect_device
Biological OsmoSampler
samp_mat_process
Methods will be published in Buser-Young, J, et al., Hidden processes during seasonal isolation of a high-altitude watershed. Submitted to Frontiers in Earth Sciences, Feb 2021. They are briefly described here. Step 1: A Biological OsmoSampler was placed in the river with tubing inlet sampling the river water column (sensu Robidart et al., 2013; Wilson et al., 2015). Step 2: Recover the Biological OsmoSampler 3 months later. Step 3: Section tubing into two ~7 m long increments and expel contents using a syringe. Step 4: Filter contents onto PES, dissolve using phenol chloroform, and extract DNA per manufacturer's instruction (Zymo Research). Step 5: amplify DNA using Earth Microbiome Protocol, sequence amplicons using Illumina MiSeq paired-end sequencing.
samp_size
There are two Biological OsmoSampler samples, each comprised of approximately 10 mL river water filtered onto PES.
NA
NA
BioSampleModel
Metagenome or environmental