Hordeum vulgare
Protocols: Uniformly sized seeds were selected, surface-sterilised, and grown under control (nutrient medium without additional NaCl) and salt-treated (nutrient medium supplemented with 100 mM NaCl) conditions as described in (Shelden et al. 2013). After three days of germination, seminal roots were dissected, collected into 1.5 mL tubes, immediately snap-frozen in liquid nitrogen, and then stored at -80°C. Samples were dissected in the following steps: A 1.5 mm long section marked Zone 1 (meristematic zone) was taken from the root tip. The second section (Zone 2) was dissected from the elongation zone up to the third section, Zone 3 (maturation zone), which was excised at the point of visible root hair elongation up to 3/4 of the entire root. Total RNA was isolated from 50 mg root tissue using the Qiagen RNeasy kit following the manufacturer's protocol. The RNA was analyzed for quality and concentration using a DeNovix DS-11 spectrophotometer (Wilmington, DE, USA) and an Agilent Technologies 2100 Bioanalyzer. Libraries were amplified through 13 cycles of PCR using Illumina guidelines. The Illumina TruSeq RNA Sample preparation kit v2 (Illumina Inc.) was used according to the manufacturer's protocol. Poly(A) enrichment was used.
ENA first public
2017-01-06
ENA last update
2016-04-20
External Id
SAMEA3935087
INSDC center alias
MU
INSDC center name
Murdoch University
INSDC first public
2017-01-06T17:01:23Z
INSDC last update
2016-04-20T10:20:02Z
INSDC status
public
Submitter Id
E-MTAB-4634:S100_Z2-1
age
3
broker name
ArrayExpress
developmental stage
0 seed germination stage
genotype
Sahara
organism part
Root elongation zone
sample name
E-MTAB-4634:S100_Z2-1