Mus musculus domesticus
Protocols: Sciatic nerves of adult mice were cut or crushed at the sciatic notch. RNA was extracted from samples using Trizol (Invitrogen) according to manufacturer's instructions with an additional purification step by on-column DNase treatment using the RNase-free DNase Kit (Qiagen) to ensure elimination of any genomic DNA. The integrity and quantity of RNA was determined using Qubit (Invitrogen) and Agilent 2100 Bioanalyzer (Agilent Technologies). Small RNA libraries were prepared using TruSeq Small RNA sample preparation kit (Illumina). Briefly, 3` and 5` indexed adapters were ligated to 1 g of total RNA and the product of this reaction was RT-PCR amplified. Small RNA library was size selected using Pippin Prep elution system (Sage Science). The quality of the libraries was evaluated using Agilent 2100 Bioanalyzer. All the libraries were pooled together and run on two lanes of the HiSeq 2000 platform to generate 50 bp single-end reads.
ENA first public
2017-08-30
ENA last update
2017-03-29
External Id
SAMEA103948141
INSDC center alias
Imperial College London
INSDC center name
Imperial College London
INSDC first public
2017-08-30T17:02:17Z
INSDC last update
2017-03-29T11:14:44Z
INSDC status
public
Submitter Id
E-MTAB-5633:cut7day_smallRNASeq_5
broker name
ArrayExpress
common name
western European house mouse
developmental stage
adult
organism part
sciatic nerve
sample name
E-MTAB-5633:cut7day_smallRNASeq_5
strain
C57BL/6J