Homo sapiens
Protocols: Primary human dermal microvascular blood and lymphatic endothelial cell lines were fixed using 1% formaldehyde and used for ChIP sequencing. Chromatin was prepared using the iDeal ChIP-Seq kit for Histones and sheared using Bioruptor Pico sonication device combined with the Bioruptor Water cooler for 12 cycles using a 30'' [ON] 30'' [OFF] settings. ChIP was performed using IP-Star Compact Automated System and antibodies against H3K27me3 and H3K4me3; chromatin (1% ) was set as Input. Libraries were prepared from input and immunoprecipitated DNA using the MicroPlex Library Preparation Kit. After library amplification, libraries were then purified using Agencourt AMPure XP (Beckman Coulter) and quantified using Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific).
ENA-FIRST-PUBLIC
2021-01-18T04:13:33Z
ENA-LAST-UPDATE
2020-04-16T11:36:09Z
External Id
SAMEA6786250
INSDC center name
PhD student, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich
INSDC first public
2021-01-18T04:13:33Z
INSDC last update
2020-04-16T11:36:09Z
INSDC status
public
Submitter Id
E-MTAB-8951:Sample 6
broker name
ArrayExpress
cell line
blood vessel endothelial cell
cell type
blood vessel endothelial cell
common name
human
developmental stage
neonate
disease
normal
genotype
wild type genotype
organism part
foreskin
replicate
2
sample name
E-MTAB-8951:Sample 6
scientific_name
Homo sapiens