Escherichia coli
source_name
Liquid culture of DH5α (Invitrogen cat-no.18265017.
treatment
100microg/ml Chloramphenicol for 5 minutes at 37C
culture conditions
ecol_cam_rep1 was grown in LB broth at 37C. Chloramphenicol treatment was initiated at exponential growth OD600 0.88.
library protocol
Standard 5PSeq
ribosomal rna delpetion strategy
Ribo-Zero rRNA Removal Kit (Bacteria) Illumina