Mus musculus
Flow-sorted pro-erythroblasts: Single cell suspensions of adult (6-8 weeks) whole spleens in PBS containing 0.5% BSA were depleted of CD45+ cells using mouse CD45 MicroBeads (Miltenyi Biotec). Cells were sorted on a FACSAria II flow cytometer (BD Biosciences) directly into lysis buffer containing -mercaptoethanol (Sigma) following staining with CD45-FITC, Ter119-PE and CD44-APC (BD Biosciences) to obtain purified erythroid precursor populations as described in Liu J et al, Blood. 2016;127(15):1856-62. Epub 2016/02/24. doi: 10.1182/blood-2016-01-694331. PubMed PMID: 26903544; PMCID: PMC4832505
strain
STOCK Klf1<Nan>/Llp
dev_stage
Adult
sex
male
tissue
spleen
biomaterial_provider
Luanne Peters, PhD, 1The Jackson Laboratory, Bar Harbor, ME 04609, USA, Luanne.Peters@jax.org
cell_type
Proerythroblast
BioSampleModel
Model organism or animal