Mus musculus
Protocols: Neural stem cells were sorted by FACS and recovered into growth media. Cells were then pelleted and lysed in RLT Buffer. Cells were cultured adherently in standard serum-free NSC growth media in the presence of 20ng/ml EGF and 10ng/ml FGF2. Cells were cultured either separately or in co-culture prior to harvesting and sorting by FACS. RNeasy extraction kit with on-column DNase digestion mRNA was isolated from total RNA using oligo dT beads. After fragmentation the sample was converted to double stranded DNA and ligated to Illumina adapters. The second strand cDNA was then degraded by the UNG enzyme providing directional information to the library. Indexing of each sample was performed by PCR.
ENA-FIRST-PUBLIC
2020-05-31T04:03:47Z
ENA-LAST-UPDATE
2019-12-10T16:13:57Z
External Id
SAMEA6378415
INSDC center name
National Heart and Lung Institute Imperial College London
INSDC first public
2020-05-31T04:03:47Z
INSDC last update
2019-12-10T16:13:57Z
INSDC status
public
Submitter Id
E-MTAB-8580:ANS7_Sep_2
broker name
ArrayExpress
cell type
neural stem cell
common name
house mouse
developmental stage
adult
disease
normal
genotype
wild type genotype
growth condition
separate
organism part
brain
phenotype
neural stem cell
sample name
E-MTAB-8580:ANS7_Sep_2
sampling site
postnatal subventricular zone
scientific_name
Mus musculus