Leishmania mexicana
AXA3, axenic amastigotes, 24h after start of differentiation Protocols: Axenic amastigotes were generated by dilution of stationary phase Leishmania mexicana promastigotes (WHO strain MNYC/BZ/62/M379) into Schneider's Drosophila medium at pH 5.5 to a density of 3x10E6 cells/ml and incubation at 34°C for 24h prior to RNA extraction. For mapping of splice acceptor sites and determination of transcript abundances, polyadenylated RNA was purified from total RNA, converted to cDNA using random hexamer primers, sheared and size selected for fragments ~200bp in length using the Illumina TruSeq RNA Sample Preparation Kit v2.
ENA first public
2015-09-11
ENA last update
2015-03-16
External Id
SAMEA3306023
INSDC center alias
Sir William Dunn School of Pathology University of Oxford
INSDC center name
Sir William Dunn School of Pathology University of Oxford
INSDC first public
2015-09-11T17:01:24Z
INSDC last update
2015-03-16T14:45:15Z
INSDC status
public
Submitter Id
E-MTAB-3312:Sample 12
broker name
ArrayExpress
developmental stage
axenic amastigote
host
not applicable (outside host)
sample name
E-MTAB-3312:Sample 12