mixed sample
AMA3, macrophages containing intracellular Leishmania, 24h post infection; used to generate library enriched for transcript 3'ends Protocols: To generate intracellular amastigotes, Leishmania mexicana promastigotes (WHO strain MNYC/BZ/62/M379) were left to grow into stationary phase (from 1x10E5 to 2x10E7 cells/ml) and then incubated with BMDMs at a parasite to macrophage ratio of 20 for 2h before washing off remaining extracellular parasites. Infected macrophages were incubated 24h at 34°C prior to RNA extraction. For determination of polyadenylation addition sites, polyadenylated RNA purified from total RNA was converted to cDNA using a 5'-T15VN-3' oligonucleotide (V=A, G or C; N=T, A, G or C) primer to enrich for 3' ends of transcripts. The cDNA was sheared and size selected for fragments ~200bp in length using the Illumina TruSeq RNA Sample Preparation Kit v2.
ENA first public
2015-09-11
ENA last update
2015-03-16
External Id
SAMEA3306029
INSDC center alias
Sir William Dunn School of Pathology University of Oxford
INSDC center name
Sir William Dunn School of Pathology University of Oxford
INSDC first public
2015-09-11T17:01:24Z
INSDC last update
2015-03-16T14:45:16Z
INSDC status
public
Submitter Id
E-MTAB-3312:Sample 14
broker name
ArrayExpress
developmental stage
amastigote
host
Mus musculus
host cell type
bone marrow macrophage
sample name
E-MTAB-3312:Sample 14
component_organism
Leishmania mexicana and Mus musculus
component_organism
mixed sample