Sus scrofa
Protocols: The C5 hiPSC was reprogrammed from the somatic cells derived from post-mortem human fetal neural tissue which was acquired at the time of surgical termination of pregnancy in the day surgery unit of Addenbrookes Hospital, Cambridge. Full informed consent was obtained from participating mothers. All generated lines are unlinked anonymous.. All ​related procedures and experiments were approved by the hospital and Sanger institute. M10 cells were derived from pig fetus fibroblast by reprogramming. Those pig fetus fibroblast cells were derived from a 28-day normal healthy male pig fetus skin. K3 cells were derived from 5-day preimplantation embryos, again, healthy male. All the related embryos and fetus experiments were approved by the Niedersaechsisches Landesamt fuer Verbraucherschutz und Lebensmittelsicherheit, LAVES, Oldenburg Germany. Abbreviations used: PFF means the pig fetus fibroblast. Emb means the origin is 5-day embryos. Parth means parthenogenetic embryos, PGCLC means the primordial germ cell-like cells differentiated from K3 cells. Total RNA was purified by RNeasy Minikit (Qiagen) according to the manufacturer's manual. RNA concentration was determined by Nanodrop, and 500 ng of total RNA was used for library construction using KAPA Stranded mRNA-Seq Kits. Sequencing was performed on Illumina HiSeq 2000 machines with 75 bp pair-end mode. KAPA Stranded mRNA-Seq Kits
ENA-FIRST-PUBLIC
2019-02-23T17:02:14Z
ENA-LAST-UPDATE
2019-02-13T13:33:42Z
External Id
SAMEA5335932
INSDC center name
Wellome Sanger Institute
INSDC first public
2019-02-23T17:02:14Z
INSDC last update
2019-02-13T13:33:42Z
INSDC status
public
Submitter Id
E-MTAB-7253:pPGCLC-K3_rep1
age
5
broker name
ArrayExpress
cell line
K3
cell type
induced primordial germ cell
common name
pig
developmental stage
embryo
disease
normal
growth condition
EPSC media
organism part
embryo
progenitor cell type
embryonic stem cell
sample name
E-MTAB-7253:pPGCLC-K3_rep1
scientific_name
Sus scrofa
sex
male