Staphylococcus epidermidis
Protocols: bacterial cultures grown to OD600 0.5, treated with 5um D-sphingosine or equivalent ethanol (lipid carrier) for 20 min, cells harvested by centrifugation and stored in RNAlater to stabilise RNA until RNA extraction bacteria grown in 50 ml THB in 250 ml flasks at 37 degrees C with shaking at 250 RPM 5um D-sphingosine or equivalent ethanol (lipid carrier) for 20 min immediately prior to cell harvesting pelleted cells lysed with lysostaphin, mutanolysin and proteinase K, RNA extracted using RNeasy kit (Qiagen) DNase treated then cleaned with RNeasy MinElute clean up kit (Qiagen) depletion of rRNA with Ribo-zero for Gram positive bacteria, RNA concentration normalised then strand specific ScriptSeq kit (Epicentre) used to generate libraries
ENA-FIRST-PUBLIC
2020-10-01T04:05:54Z
ENA-LAST-UPDATE
2020-06-29T17:20:39Z
External Id
SAMEA7026312
INSDC center name
UNIVERSITY OF MANCHESTER
INSDC first public
2020-10-01T04:05:54Z
INSDC last update
2020-06-29T17:20:39Z
INSDC status
public
Submitter Id
E-MTAB-9272:SE_Tu3298_con2
broker name
ArrayExpress
sample name
E-MTAB-9272:SE_Tu3298_con2
scientific_name
Staphylococcus epidermidis
strain
Tu3298