Mus musculus
Protocols: Mice were treated at ZT6 (6 hours after lights on, 1:30pm) or at ZT18 (6 hours after lights off, 1:30am) with dexamethasone (1 mg/kg intraperitoneal) or vehicle (methylcyclodextrin 1mg/kg intraperitoneal) for 2 hours before sacrifice by cervical dislocation. Liver sections from mouse were used for RNASeq study. Tophat-2.0.11 Liver tissue were lysed and total RNA prepared using SV Total RNA Isolation System (Promega). RNA quality was checked using the RNA 6000 Nano Assay, RNA samples with a 260:280 nM ratio of ~2 taken forward for analysis. Quality and integrity of total RNA samples were assessed using a 2100 Bioanalyzer or a 2200 TapeStation (Agilent Technologies) according to the manufacturer's instructions. polyadenylated mRNA was purified using poly-Toligo-attached, magnetic beads. TruSeq® Stranded mRNA assay (Illumina, Inc.) according to the manufacturer's protocol
ENA-FIRST-PUBLIC
2018-11-13T17:03:37Z
ENA-LAST-UPDATE
2018-07-18T14:11:01Z
External Id
SAMEA4796453
INSDC center name
UNIVERSITY OF MANCHESTER
INSDC first public
2018-11-13T17:03:37Z
INSDC last update
2018-07-18T14:11:01Z
INSDC status
public
Submitter Id
E-MTAB-7017:GC8
age
10 to 20
broker name
ArrayExpress
common name
house mouse
disease
normal
genotype
wild type genotype
organism part
liver
sample name
E-MTAB-7017:GC8
scientific_name
Mus musculus
sex
male
strain
C57BL/6