Mus musculus
Pax6-cKO heterozygous control animals carry only one allele of floxed Pax6 in Emx-1-positive cortical cells. This means only one copy of Pax6 was deleted to produce truncated PAX6 proteins. At E14.5, the tissue was treated with Worthington papain dissociation system to produce single-cell suspension. About 4,000 GFP-positive cells were purified using FACS. Isolated GFP-positive cells were loaded into 10X genomics Chromium Controller to produce single-cell transcriptome libraries using Chromium Single Cell 3' Reagent Kits.
ENA-FIRST-PUBLIC
2020-10-01T04:05:37Z
ENA-LAST-UPDATE
2019-05-24T12:16:09Z
External Id
SAMEA5658324
INSDC center name
ED
INSDC first public
2020-10-01T04:05:37Z
INSDC last update
2019-05-24T12:16:09Z
INSDC status
public
Submitter Id
E14HET
cell type
Rostro-dorsal telencephalon neural cells
collection date
2019-04-12
common name
house mouse
dev stage
E14.5
sample name
E14HET
scientific_name
Mus musculus