Homo sapiens
Protocols: Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation from blood samples of VPER and VRIS using Ficoll-Paque™. PBMC cells (106 cells) were seeded in 24-well plates in RPMI1640 supplemented with fetal calf serum (FCS), penicillin, streptomycin, amphotericin B (all from Invitrogen) and stimulated with LPS (1 µg/mL; from Salmonella Abortus equi ; Sigma, Saint-Quentin-Fallavier, France) for 3 h or 6 h at 37 °C in a humidified atmosphere containing 5% CO2. The control condition without LPS stimulation is named mi. Description of the sample naming: lps: stimualtion with lps - mi: no stimulation - 3h/6h: lps stimulation for 3 or 6 hours - _1 or _2: replicate 1 or 2 - VPER: subject VPER - VRIS: subject VRIS Trizol SOLiD Total RNA-Seq Kit
ENA-FIRST-PUBLIC
2018-03-23T17:02:19Z
ENA-LAST-UPDATE
2018-03-13T14:31:16Z
External Id
SAMEA104692602
INSDC center name
University of Strasbourg
INSDC first public
2018-03-23T17:02:19Z
INSDC last update
2018-03-13T14:31:16Z
INSDC status
public
Submitter Id
E-MTAB-6563:VRIS_lps6h37_2
age
45
broker name
ArrayExpress
cell type
mononuclear cell
common name
human
disease
hyper IgD syndrome
individual
2
organism part
blood
phenotype
hyper IgD syndrome symptoms
sample name
E-MTAB-6563:VRIS_lps6h37_2
scientific_name
Homo sapiens
sex
female