Mus musculus
Pax6-cKO homozygous control animals carry two copies Pax6-floxed alleles in Emx-1-positive cortical cells. A complete deletion of Pax6 is normally seen from E12.5 onward. At E14.5, the tissue was treated with Worthington papain dissociation system to produce single-cell suspension. About 4,000 GFP-positive cells were purified using FACS. Isolated GFP-positive cells were loaded into 10X genomics Chromium Controller to produce single-cell transcriptome libraries using Chromium Single Cell 3' Reagent Kits.
ENA-FIRST-PUBLIC
2020-10-01T04:05:37Z
ENA-LAST-UPDATE
2019-05-24T12:16:09Z
External Id
SAMEA5658325
INSDC center name
ED
INSDC first public
2020-10-01T04:05:37Z
INSDC last update
2019-05-24T12:16:09Z
INSDC status
public
Submitter Id
E14HOM
cell type
Rostro-dorsal telencephalon neural cells
collection date
2019-04-12
common name
house mouse
dev stage
E14.5
sample name
E14HOM
scientific_name
Mus musculus