Mus musculus
Protocols: The CD8α+ DC lines were grown in IMDM medium supplemented with 10% FBS and antibiotic solution (Gibco), 5x10-5M 2-mercaptoethanol,sodiumbicarbonate, hepes. The cells were maintained at 37°C in a humidified incubator with 5% CO2. DNA-Chromatin was fixed and cross-linked using 16% formaldehyde and quenched using 2.5M glycine. Thereafter, the fixed and cross-linked samples were fragmented by sonication in a Bioruptor. the fragments between 200 to 500 bp was further processed and immuno-precipitated with Zeb1 Antibody and checked with qPCR. Rest of the samples were preserved for preparing library and sequencing. For ChIP-seq library preparation, 30 µl ChIP-DNA was processed for library preparation according to ChIP-seq library preparation recommended protocol (NEB)
ENA-FIRST-PUBLIC
2018-11-13T17:03:36Z
ENA-LAST-UPDATE
2018-06-18T21:07:20Z
External Id
SAMEA4743666
INSDC center name
Institute of Life Sciences, Nalco Square Chandrashekharpur, Bhubaneswar Odisha-751023
INSDC first public
2018-11-13T17:03:36Z
INSDC last update
2018-06-18T21:07:20Z
INSDC status
public
Submitter Id
E-MTAB-6917:Zeb1 0hr ChIP
broker name
ArrayExpress
cell type
conventional dendritic cell
common name
house mouse
sample name
E-MTAB-6917:Zeb1 0hr ChIP
scientific_name
Mus musculus