Saccharomyces cerevisiae
Protocols: Samples were collected at mid-exponential growth phase for transcriptomics, proteomics and extracellular metabolomics. For total RNA, 10 mL of culture broth was collected in a 50 mL Falcon® tube filled with ice and immediately centrifuged at 10.000rpm for 2 min at 0 °C. After centrifugation, cell pellet were snap-frozen in liquid nitrogen and kept at -80°C until extraction. Pre-cultures were prepared by cultivating a single colony from a selective YPD plate of each yeast strain in YPD medium until mid-exponential phase (~16 h). Pre-cultured cells were centrifuged, washed two times with distilled water, and inoculated in defined minimal media with 2% glucose at initial OD600 of 0.1. These cultivations were performed in triplicates. Total RNA was isolated with RNAeasy kit (Qiagen) following manufacturer recommendations. To remove DNA contamination, samples were digested with Turbo DNAse (Invitrogen Ambion) followed by RNA clean-up (RNAeasy kit, Qiagen). RNA library was prepared using the NEBNext® Ultra™ II Directional RNA Library Preparation Kit for Illumina: polyA transcripts capture. Barcoded stranded mRNA-seq libraries were prepared from ~200 ng total RNA samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich, MA, USA).
ENA-FIRST-PUBLIC
2020-10-01T04:05:58Z
ENA-LAST-UPDATE
2020-08-28T16:04:53Z
External Id
SAMEA7280134
INSDC center name
Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
INSDC first public
2020-10-01T04:05:58Z
INSDC last update
2020-08-28T16:04:53Z
INSDC status
public
Submitter Id
E-MTAB-9499:E_Chassis_F 3
broker name
ArrayExpress
common name
baker's yeast
genotype
MAT URA3 HIS3 LEU2 TRP1 MAL2-8c SUC2 ser3 ser33 fum1
phenotype
fumarate producer
replicate
3
sample name
E-MTAB-9499:E_Chassis_F 3
scientific_name
Saccharomyces cerevisiae
strain
E_Chassis_F