Mus musculus
Protocols: Tissue from ventricles of male adult mice was enzymatically digested into a single cell suspension and viable non-myocte cells were isolated using FACS sorting. Briefly, hearts were minced and incubated in collagenase type II (Worthington, USA) at 37°C before being filtered on 40μm strainers. Cells were then resuspended in red cell lysis buffer followed by dead cell removal and live cells (DAPI-) were FACS sorted and counted to be loaded on to the 10x Chromium microfluidics chip (3-prime V2). Cells were loaded on the 10x Chromium system and single cell RNA-seq libraries were generated following the manufacturer's instructions. 10x Chromium 3-prime V2 protocol 10x Chromium 3-prime V2 protocol
ENA-FIRST-PUBLIC
2020-05-31T04:03:39Z
ENA-LAST-UPDATE
2019-05-16T11:10:28Z
External Id
SAMEA5610128
INSDC center name
VICTOR CHANG CARDIAC RESEARCH INSTITUTE
INSDC first public
2020-05-31T04:03:39Z
INSDC last update
2019-05-16T11:10:28Z
INSDC status
public
Submitter Id
E-MTAB-7971:PDGF day 3 sham
age
8
broker name
ArrayExpress
cell type
cardiac non-myocyte
common name
house mouse
developmental stage
adult
genotype
wild type genotype
injury
sham
organism part
cardiac ventricle
sample name
E-MTAB-7971:PDGF day 3 sham
scientific_name
Mus musculus
sex
male
stimulus
platelet-derived growth factor AB; 10ng/hr for 3 days
strain
C57BL/6J