Mus musculus
Protocols: Experimentation was performed on mouse strains C57BL/6J (WT) from Harlan Blackthorn, UK. Mice were treated at 6 hours ZT6 (6 hours after lights on, 1:30pm) or at ZT18 (6 hours after lights off, 1:30am) with dexamethasone (1 mg/kg intraperitoneal) or vehicle (methylcyclodextrin 1mg/kg intraperitoneal) Lung and, liver were lysed and total RNA prepared using SV Total RNA Isolation System (Promega). Quality and integrity of total RNA samples were assessed by 2100 Bioanalyzer or a 2200 TapeStation (Agilent Technologies) according to the manufacturer's instructions. RNA sequencing (RNA-seq) libraries were generated using the TruSeq® Stranded mRNA assay (Illumina, Inc.) according to the manufacturer's protocol.
ENA-FIRST-PUBLIC
2018-11-13T17:03:37Z
ENA-LAST-UPDATE
2018-07-18T16:37:40Z
External Id
SAMEA4796584
INSDC center name
Division of Informatics, Imaging and Data Sciences Faculty of Biology, Medicine, and Health University of Manchester, Manchester, UK
INSDC first public
2018-11-13T17:03:37Z
INSDC last update
2018-07-18T16:37:40Z
INSDC status
public
Submitter Id
E-MTAB-7020:Sample 4
age
10 to 20
broker name
ArrayExpress
common name
house mouse
disease
normal
genotype
wild type genotype
organism part
lung
sample name
E-MTAB-7020:Sample 4
scientific_name
Mus musculus
sex
male
strain
C57BL/6J