Arabidopsis thaliana
biomaterial_provider
Coruzzi lab, New York University
tissue
shoots
age
3 weeks old
growth_protocol
WT and sdg8-5 (previously named cli186) seeds were surface sterilized and imbibed in darkness for 2 days. Plants were then grown hydroponically inside a sterile Phytatray (Sigma-Aldrich, St. Louis, MO, USA) on liquid Basal MS medium (GIBCO Formula# 97-5068EC) supplemented with 1% sucrose and 2 mM KNO3 at a pH of 5.7. The phytatrays were kept under white light (50 uE m-2s-1) in long-day cycle (16h Light/8h Dark) at 220C for 3 weeks (when they start flowering). After 3 weeks, the plants were starved in liquid basal MS medium (GIBCO Formula# 97-5068EC) supplemented with 0% sucrose and 2 mM KNO3 at a pH of 5.7 and the phytatrays were covered with aluminum foil (for light starvation) for 24 hours. Plants were then treated with +/- 1% Sucrose and +/- Light (70 uEin m-2s-1) for 2 hours.
geo_loc_name
missing
ecotype
Columbia
collection_date
missing
BioSampleModel
Plant