Mus musculus
Protocols: Cells were harvested by trypsinization Naïve J1 mouse ESCs were cultured and differentiated into EpiLCs as described previously (Hayashi and Saitou, 2013; Mulholland et al., 2015). In brief, for both naïve ESCs and EpiLCs defined media was used, consisting of N2B27: 50% neurobasal medium (Life Technologies), 50% DMEM/F12 (Life Technologies), 2 mM L-glutamine (Life Technologies), 0.1 mM β-mercaptoethanol (Life Technologies), N2 supplement (Life Technologies), B27 serum-free supplement (Life Technologies), 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma). Naïve ESCs were maintained on flasks treated with 0.2% gelatin in defined media containing 2i (1 μM PD032591 and 3 μM CHIR99021 (Axon Medchem, Netherlands)), 1000 U/mL recombinant leukemia inhibitory factor (LIF, Millipore), and 0.3% BSA (Gibco) for at least three passages before commencing differentiation. column purification SCRB-seq adapted for bulk samples (Ziegenhain, C., Vieth, B., Parekh, S., Reinius, B., Guillaumet-Adkins, A., Smets, M., Leonhardt, H., Heyn, H., Hellmann, I., Enard, W., 2017. Comparative Analysis of Single-Cell RNA Sequencing Methods. Mol. Cell 65, 631-643.e4.)
ENA-FIRST-PUBLIC
2020-06-29T04:03:40Z
ENA-LAST-UPDATE
2019-10-21T16:29:53Z
External Id
SAMEA6104710
INSDC center name
Faculty of Biology Department II Ludwig Maximilians University Munich
INSDC first public
2020-06-29T04:03:40Z
INSDC last update
2019-10-21T16:29:53Z
INSDC status
public
Submitter Id
E-MTAB-6797:wt_ESC_rep4
broker name
ArrayExpress
cell line
J1
cell type
naive embryonic stem cell
common name
house mouse
developmental stage
blastocyst
genotype
wild type genotype
organism part
inner cell mass
sample name
E-MTAB-6797:wt_ESC_rep4
scientific_name
Mus musculus
strain
129S4/SvJae