Drosophila melanogaster
MyoIAts > UAS-dIAP Protocols: MyoIAts was used to drive transgene expression for 24 hours at 29 degrees C. 25 adult female intestines per genotype were dissected and midguts were homogenized in Trizol (Life Technologies). RNA was isolated by Phenol/Chloroform extraction, followed by purification of the RNA containing fractions using RNAeasy columns supplied with the RNAeasy Mini Kit (Qiagen). RNA was Poly-A-selected. RNA integrity was determined with an Agilent 2100 Bioanalyzer before library generation. cDNA libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB), and NEBNext Multiplex Oligos for Illumina (NEB) were used for multiplexing.
ENA-FIRST-PUBLIC
2014-10-25T17:01:04Z
ENA-LAST-UPDATE
2018-03-08T21:08:52Z
External Id
SAMEA2765961
INSDC center name
DKFZ/ZMBH Alliance, University of Heidelberg, Heidelberg, Germany
INSDC first public
2014-10-25T17:01:04Z
INSDC last update
2018-03-08T21:08:52Z
INSDC status
public
Submitter Id
E-MTAB-2915:C1 UAS-dIAP no1
broker name
ArrayExpress
common name
fruit fly
genotype
UAS-dIAP overexpression
sample name
E-MTAB-2915:C1 UAS-dIAP no1
scientific_name
Drosophila melanogaster