Mus musculus
BMDM3, control, uninfected macrophages Protocols: Bone marrow cells were harvested from C57BL/6 mice and matured in vitro in 20% L929 conditioned medium, resulting in cells expressing the murine macrophage markers F4/80 and MAC-1, whilst being negative for the granulocyte marker GR-1, as assessed by flow-cytometry. Macrophages were then cultured for 2 days in Dulbecco's Modified Eagle Medium (DMEM) before the start of the experiment. For mapping of splice acceptor sites and determination of transcript abundances, polyadenylated RNA was purified from total RNA, converted to cDNA using random hexamer primers, sheared and size selected for fragments ~200bp in length using the Illumina TruSeq RNA Sample Preparation Kit v2.
ENA-FIRST-PUBLIC
2015-09-11T17:01:24Z
ENA-LAST-UPDATE
2018-03-08T23:43:18Z
External Id
SAMEA3306026
INSDC center name
Sir William Dunn School of Pathology University of Oxford
INSDC first public
2015-09-11T17:01:24Z
INSDC last update
2018-03-08T23:43:18Z
INSDC status
public
Submitter Id
E-MTAB-3312:Sample 8
broker name
ArrayExpress
common name
house mouse
host
not applicable (uninfected control)
sample name
E-MTAB-3312:Sample 8
scientific_name
Mus musculus