Leishmania mexicana
AXA2, axenic amastigotes, 24h after start of differentiation; used to generate library enriched for transcript 3'ends Protocols: Axenic amastigotes were generated by dilution of stationary phase Leishmania mexicana promastigotes (WHO strain MNYC/BZ/62/M379) into Schneider's Drosophila medium at pH 5.5 to a density of 3x10E6 cells/ml and incubation at 34°C for 24h prior to RNA extraction. For determination of polyadenylation addition sites, polyadenylated RNA purified from total RNA was converted to cDNA using a 5'-T15VN-3' oligonucleotide (V=A, G or C; N=T, A, G or C) primer to enrich for 3' ends of transcripts. The cDNA was sheared and size selected for fragments ~200bp in length using the Illumina TruSeq RNA Sample Preparation Kit v2.
ENA first public
2015-09-11
ENA last update
2015-03-16
External Id
SAMEA3306027
INSDC center alias
Sir William Dunn School of Pathology University of Oxford
INSDC center name
Sir William Dunn School of Pathology University of Oxford
INSDC first public
2015-09-11T17:01:24Z
INSDC last update
2015-03-16T14:45:16Z
INSDC status
public
Submitter Id
E-MTAB-3312:Sample 17
broker name
ArrayExpress
developmental stage
axenic amastigote
host
not applicable (outside host)
sample name
E-MTAB-3312:Sample 17