Homo sapiens
Protocols: MScs purchased from young and old human donors miRNAeasy kit for RNA extraction Total RNA samples were ribosomal RNA depleted using the Human, mouse, rat low inputRibozero kit from Epicentre. 1ug of RNA (or the max amount available) was mixed with the low input kit baits and allowed to anneal. This was then mixed with the magnetic microspheres which bind the ribosomal complexes and subsequently pulled out with a magnet. The remaining RNA was purified with RNA clean beads and analysed with the Agilent Bioanalyser with a RNA pico chip. Recovery was generally less than 1% of the original starting material. All of this material was used in the script seq reaction (Epicentre). Samples were denatured and annealed to cDNA primers and cDNA was generated. This was amplified with indexed primers for 15 cycles to make the library for sequencing. Libraries were checked on the Agilent Bioanalyser with a High sensitivity chip and quantities determined with Qubit DNA High sensitivity reagents.
ENA first public
2017-01-06
ENA last update
2016-07-04
External Id
SAMEA4062954
INSDC center alias
Musculoskeletal Biology, Institute of Ageing and Chronic Disaese, Leahurst, Chester High Road, Neston, Wirral, CH647TE
INSDC center name
Musculoskeletal Biology, Institute of Ageing and Chronic Disaese, Leahurst, Chester High Road, Neston, Wirral, CH647TE
INSDC first public
2017-01-06T17:01:24Z
INSDC last update
2016-07-04T22:45:30Z
INSDC status
public
Submitter Id
E-MTAB-4879:Sample 20
broker name
ArrayExpress
cell type
tenocyte
common name
human
developmental stage
young adult
sample name
E-MTAB-4879:Sample 20