Mus musculus
Protocols: Fecal content was collected from gut region encompassing distal cecum and approximately one inch of the colon and frozen on dry ice. The fecal matter was lysed using glass beads in MagnaLyser tissue disruptor (Roche) and total DNA isolated using Power-soil/fecal DNA isolation kit (Mo-Bio) as per manufacturer's specifications. All samples was quantified via the Qubit® Quant-iT dsDNA Broad- Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA and submitted to University of Minnesota Genomic Center for 16s rRNA sequencing . Using QIIME, sequences were quality filtered and demultiplexed using exact matches to the supplied DNA barcodes.
ENA-FIRST-PUBLIC
2020-01-11T04:05:38Z
ENA-LAST-UPDATE
2019-07-03T11:58:08Z
External Id
SAMEA5752917
INSDC center name
Surgery/Surgical Oncology, University of Miami
INSDC first public
2020-01-11T04:05:38Z
INSDC last update
2019-07-03T11:58:08Z
INSDC status
public
Submitter Id
E-MTAB-7883:Sample 6
age
12
broker name
ArrayExpress
common name
house mouse
disease
normal
genotype
wild type genotype
individual
mouse_6
organism substance
feces
sample name
E-MTAB-7883:Sample 6
scientific_name
Mus musculus
treatment
placebo