Homo sapiens
source_name
Purified Murine-Leukemia Virus-like particles loaded with the Cas9 protein and a single-guideRNA targeting the YFP coding sequence.
cell line
HEK293T Lenti-X
transfection
plasmids coding for MLV Gag/Pro-Pol, a Gag::Cas9 fusion, a single-guideRNA targeting the YFP coding sequence, VSVG, BaeV
purification
virus-like particles purified on a sucrose cushion