Mus musculus
Protocols: Sertoli cells were isolated from testes of 6 dpp mice and maintained in DMEM/F12 media supplemented with 2.5%FBS, 5%horse serum, 2mML-Gln, 100U/mLpenicillin, 1uMsodium pyruvate Sertoli cells lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Cells were cross-linked with 1% formaldehyde for 10 min at room temperature and formaldehyde was then inactivated by the addition of 125 mM glycine for 5min. Sonicated DNA fragments with 100-300 bp were pre-cleared and then immunoprecipitated with Protein A+G Magnetic beads coupled with Anti-Androgen Receptor antibody (06-680, Millipore).
ENA-FIRST-PUBLIC
2018-08-20T17:02:03Z
ENA-LAST-UPDATE
2018-08-10T10:27:52Z
External Id
SAMEA4825580
INSDC center name
School of biomedical engineering,Shanghai Jiao Tong University
INSDC first public
2018-08-20T17:02:03Z
INSDC last update
2018-08-10T10:27:52Z
INSDC status
public
Submitter Id
E-MTAB-7129:ChIP-seq-androgen-SSC
age
6
broker name
ArrayExpress
cell type
Sertoli cell
common name
house mouse
genotype
wild type genotype
organism part
testis
sample name
E-MTAB-7129:ChIP-seq-androgen-SSC
scientific_name
Mus musculus
sex
male
strain
SSC