iCLIP-seq of G3BP1 in N-expressing cells, replicate A
source_name
Human embryonic kidney cells
cell line
HEK293 cells overexpressing SARS-CoV-2 N
treatment
GFP-N was expressed in an HEK293 cell line engineered with the Flp-in system under the control of doxycyclin, and expression was induced for 24 hours prior to UV crosslinking.
antibody
G3BP1 monoclonal antibody (SANTA CRUZ Biotechnology)