nmt-seb1-IP-1
Protocols: S. pombe cells were grown in EMM medium supplemented with 20 ug/ml Thiamine at 30C for 10 hours (final OD600: 0.5-0.6). ChIP experiments were performed as described in (Larochelle et al. NAR 2012, 40:10240). For ChIP-seq experiments, chromatin immunoprecipitation was done as described above with the following modifications. We scaled up the reactions to obtain larger amount of chromatin for library preparation. Chromatin was immunoprecipitated with Pan mouse IgG Dynabeads (Invitrogen) antibody against Rpb1 (8WG16, Convance). The 8WG16 antibody is known to recognize the C-terminal domain (CTD) of Rpb1 and measures total RNAPII along genes in yeast. Whole cell extract from fixed cells was prepared as described in (Larochelle et al. NAR 2012, 40:10240) Libraries were prepared using the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina kit (New England BioLabs) according to the manufacturer instructions.
ENA first public
2016-07-31
ENA last update
2016-06-20
External Id
SAMEA4041044
INSDC center alias
MRC Clinical Sciences Centre. Imperial College London
INSDC center name
MRC Clinical Sciences Centre. Imperial College London
INSDC first public
2016-07-31T17:03:05Z
INSDC last update
2016-06-20T16:24:37Z
INSDC status
public
Submitter Id
E-MTAB-4827:nmt-seb1-IP-1
biological repeat
1
broker name
ArrayExpress
common name
fission yeast
exepriment_id
2202_5
genotype
h+ ade6? leu1-32 ura4-D18 his3-D1 P41nmt1-seb1::kanMX6
sample name
E-MTAB-4827:nmt-seb1-IP-1
strain
FBY915