D17_Healthy Cell 03
Protocols: One cryotube (1.7 million cells) of the M398 healthy cell line was thawed in a T75 BioCoat™ Collagen I cultureware (356485, Corning Incorporated) with SKM02 medium (AMSbio). Cells were harvested with trypsin the following day. 1.5 million cells were centrifuged at 300 g for 5 minutes. Cells were resuspended at 1,500 cells/µL in DMEM F-12 with 10% serum and processed according to the 10X Genomics single-cell RNA sequencing protocol. NA The library was prepared with Chromium™ Single Cell 3' Library & Gel Bead Kit v2.
ENA-FIRST-PUBLIC
2020-10-04T04:06:15Z
ENA-LAST-UPDATE
2020-09-04T10:49:53Z
External Id
SAMEA7290525
INSDC center name
INSERM UEVE UMR861, I-STEM, AFM
INSDC first public
2020-10-04T04:06:15Z
INSDC last update
2020-09-04T10:49:53Z
INSDC status
public
Submitter Id
E-MTAB-9510:D17_Healthy Cell 03
broker name
ArrayExpress
cell line
M398
cell type
skeletal muscle cell
common name
human
developmental stage
adult
disease
normal
genotype
wild type genotype
progenitor cell type
induced plurioptent stem cell
sample name
E-MTAB-9510:D17_Healthy Cell 03
sampling time point
17
scientific_name
Homo sapiens