Brassica napus cv. Darmor-PBY018 plants were cultivated B-sufficient soil substrate in the glasshouse. A B-free white-peat volcanic clay mixture (as from now called zerosoil-substrate) was supplemented with 0.5% CaCO3 and 0.3% CaO by mixing 20 kg batches of soil with 1 L of CaCO3 (100 g L-1) and 1 L of CaO (60 g L-1) in a clean cement mixer (Lescha Typ SBM P 150L) for 10 min. Thereafter, soil substrate was manually mixed every other day. Around 1-2 weeks after preparation, clumps were sieved out and the soil (dry matter: ~ 30 %) was used for the experiments. Rapeseed plants were germinated directly in individual pots (9 cm) filled with zerosoil-substrate in the glasshouse. Pots were watered every two days with 250 ml 1x modified Hoagland solution containing 200 ��M boric acid. All solutions additionally contained the following concentrations of nutrients: 7.5 mM NH4NO3, 2.9 mM KH2PO4, 850 ��M MgSO4, 340 ��M K2SO4, 320 ��M MnCl2, 52 ��M CuSO4, 20 ��M NaFeEDTA, 4.5 ��M ZnSO4, 0.4 ��M NaMoO4. At BBCH15, plants were transplanted into new pots (16 cm) with fresh zerosoil-substrate. Plants under B-sufficient conditions were maintained under the same watering and fertilization regime as above. Nutrients were supplied to the soil weekly. Care was taken to avoid transfer of soil by roots during transplantation. Plants were then subjected to vernalization for six weeks at 6��C, and finally transferred to the glasshouse. No glassware was used in any process during the preparation of the nutrient solution or the irrigation of the plants to prevent additional input of B into the growth system. Glasshouse conditions were set to long-day conditions. Plant tissues from three individual plants per B growth condition were harvested for RNA isolation. At BBCH 51, the shoot head (including the shoot apical meristem and the forming flower buds) as well as 2-3 bracts were sampled.
air temperature regimen
22/18 degree Celsius
biological material altitude
112 m
biological material geographic location
Germany
biological material latitude
51.816
biological material longitude
11.283
biological material ploidy
allopolyploidy
checklist
BSDC00001
collection date
2017-07-31
cultivar
Darmor-PBY018
genus
Brassica
geographic location (country and/or sea)
not collected
growth facility
greenhouse
humidity regimen
50/60% relative humidity
light intensity
250 ��mol/m2 s
light regimen
16h light/8h dark
material source geographic location
not collected
plant anatomical entity
flower
plant growth medium
B-free white-peat volcanic clay mixture
plant structure development stage
BBCH60-61
project
Boron deficiency responsive genes in Brassica napus inflorescences
replicate
3
rooting medium micronutrients
Pots were watered every two days with 250 ml 1x modified Hoagland solution containing 200 ��M boric acid. All solutions additionally contained the following concentrations of nutrients: 7.5 mM NH4NO3, 2.9 mM KH2PO4, 850 ��M MgSO4, 340 ��M K2SO4, 320 ��M MnCl2, 52 ��M CuSO4, 20 ��M NaFeEDTA, 4.5 ��M ZnSO4, 0.4 ��M NaMoO4. At BBCH15, plants were transplanted into new pots with fresh soil. Plants under B-sufficient conditions were maintained under the same watering and fertilization regime as above. Plants under B-deficient conditions were treated in the same way but without the addition of any B. Nutrients were supplied to the soil weekly.
sample id
IPK_LIMS:1743384
sample name
plusB/Flower/BBCH60-61plus4days
species
napus
treatment
plus Bor