Sample 1
Protocols: PBMC from donor peripheral blood. Then positive magnetic bead-based isolation (CD4 or CD8 cells) or FACS sorting. Phenol-chloroform extraction followed by ethanol precipitation 5'-RACE cDNA synthesis with UMI introduced with universal adapter followed by 2-step PCR amplification
ENA-FIRST-PUBLIC
2020-10-04T04:06:16Z
ENA-LAST-UPDATE
2020-09-24T12:10:22Z
External Id
SAMEA7335804
INSDC center name
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
INSDC first public
2020-10-04T04:06:16Z
INSDC last update
2020-09-24T12:10:22Z
INSDC status
public
Submitter Id
E-MTAB-9601:Sample 1
age
20
broker name
ArrayExpress
cell type
T cell
common name
human
developmental stage
adult
disease
normal
individual
1
organism part
blood
phenotype
bulk
sample name
E-MTAB-9601:Sample 1
scientific_name
Homo sapiens
sex
male