nax18
collection_date
2019-04-06
env_broad_scale
tropical biome [ENVO:01001830]
env_local_scale
tropical marine coral reef biome [ENVO:01000854]
env_medium
environmental pollution [ENVO:02500036]|thermal pollution [ENVO_01001265]
geo_loc_name
Germany: Uni Bremen
host
Xenia umbellata
lat_lon
53.6 N 8.51 E
perturbation
chemical and physical disturbance
samp_collect_device
plastic ziplock bag
samp_mat_process
storing in seawater
samp_size
100 mg
temp
temperature of 26 degree
chem_administration
10mg/L DOC
description
exposure for 21 days
host_taxid
1366487
nucl_acid_ext
https://files.zymoresearch.com/protocols/_d4068_d4069_quick-dna_miniprep_plus_kit.pdf
lib_const_meth
paired-end
target_gene
16S rRNA
target_subfragment
V3V4
pcr_primers
FWD:AGGATTAGATACCCTGGTA;REV:CRRCACGAGCTGACGAC
pcr_cond
initial pre-denaturation:96degC_1min, denaturation:96degC_15s, annealing=55degC_30s, extension=70degC_90s, hold at 8 degC
seq_meth
Illumina Miseq
BioSampleModel
MIMARKS.survey
BioSampleModel
MIGS/MIMS/MIMARKS.host-associated