Egg
Protocols: Tetraodon embryos were obtained by in vitro fertilization of eggs and their development was observed through brightfield microscopy. Total RNA was extracted with Trizol (Invitrogen) according to the manufacturer s protocol from eggs, whole embryo at 30% epiboly (30 epi) and whole embryo at 24 hours post fertilisation (24 hpf). The RNA samples were treated with 2U Dnase I (Qiagen) per μg RNA sample at 37°C for 10 minutes. Digested samples were then treated with 20 mg/mL proteinase K (Sigma Aldrich) at 37°C for 45 minutes. The quality and quantity of total RNA were assessed with the Bioanalyzer 2100 (Agilent) and no sign of degradation was detected (RIN > 9.0). Sequencing libraries were generated from total RNA samples following the Truseq RNA protocol (Illumina).
ENA-FIRST-PUBLIC
2016-07-29T17:01:10Z
ENA-LAST-UPDATE
2018-03-09T02:18:18Z
External Id
SAMEA3497893
INSDC center name
Stazione Zoologica Anton Dohrn Computational Genomics Biology and Evolution of Marine Organisms
INSDC first public
2016-07-29T17:01:10Z
INSDC last update
2018-03-09T02:18:18Z
INSDC status
public
Submitter Id
E-MTAB-3804:Egg
broker name
ArrayExpress
common name
spotted green pufferfish
developmental stage
unfertilized egg
organism part
egg
sample name
E-MTAB-3804:Egg
scientific_name
Tetraodon nigroviridis