A549 BR1
Protocols: F12K + 10% FBS Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced
ENA first public
2016-05-10
ENA last update
2016-05-09
External Id
SAMEA3959771
INSDC center alias
Glaxo Smithkline
INSDC center name
Glaxo Smithkline
INSDC first public
2016-05-10T17:04:10Z
INSDC last update
2016-05-09T14:41:58Z
INSDC status
public
Submitter Id
E-MTAB-4729:A549 BR1
broker name
ArrayExpress
cell line
A549
common name
human
disease
non-small cell lung carcinoma
sample name
E-MTAB-4729:A549 BR1