P17_CP
Protocols: Mononuclear cells were isolated from peripheral blood or bone marrow using Ficoll. Genomic DNA (gDNA) was isolated from MNCs using QIAsymphony DSP DNA Midi Kit (Qiagen), MspI (New England Biolabs, NEB) digested, end-repaired and A-tailed using Klenow Polymerase (NEB) and dATP, dCTP and dGTP. Illumina adapters were ligated using Quick Ligase (NEB) followed by AMPure XP bead size selection (Beckman Coulter). RRBS libraries were bisulfite treated using EZ DNA MethylationDirect kit (Zymo Research) followed by quantification by qPCR. Enrichment PCR was performed using PfuTurbo Cx Hotstart kit (Agilent Technologies) followed by AMPure XP bead clean up. Quality of final libraries was checked by Experion analysis (BioRad).
ENA first public
2016-08-01
ENA last update
2016-01-25
External Id
SAMEA3856264
INSDC center alias
Medical University of Vienna
INSDC center name
Medical University of Vienna
INSDC first public
2016-08-01T17:01:06Z
INSDC last update
2016-01-25T17:33:09Z
INSDC status
public
Submitter Id
E-MTAB-4341:P17_CP
broker name
ArrayExpress
common name
human
disease
chronic myeloid leukemia
disease staging
chronic phase chronic myeloid leukemia
sample name
E-MTAB-4341:P17_CP