iCLIP-seq of G3BP1, replicate A
source_name
Human embryonic kidney cells
cell line
HEK293
growth protocol
Human embryonic kidney cells (HEK293) cells were grown in DMEM supplemented with 10% FBS, sodium pyruvate, MEM non-essential amino acids, and penicillin/streptomycin, and maintained at 37°C with 5% CO2.
treatment
WT cells were UV crosslinked prior to harvesting
antibody
G3BP1 monoclonal antibody (SANTA CRUZ Biotechnology)