donor6_S2_6
Protocols: Physiologically normal human liver tissues were collected from the periphery of liver specimens from donors undergoing surgical resection. Liver tissue was stored at 4 °C and processed within 3 h post resection. Liver non-parenchymal cells (NPCs) were isolated following a two-step protocol of collagenase/EDTA digestion. Briefly, hepatocytes were removed by centrifuging the whole digestion at 50 g for 5 min (pellet removed - hepatocyte fraction, cleaned on Percoll), and the supernatant removed and spun again at 50 g for 5 min to remove the remaining hepatocytes, the supernatant was then pelleted at 300 g for 8 min and used as NPC fraction for downstream sequencing library preparation. Cell viability of the isolated NPCs was estimated by trypan blue exclusion. Samples of 67-89% viable cells from each of the liver samples were subjected to single-cell RNA sequencing using the 10x Genomics Chromium Single Cell 3' Library & Gel Bead Kit v2 following the manufacturer's instructions. Approximately 4000 cells were targeted for each sample recovery.
ENA first public
2021-11-05
ENA last update
2021-11-05
External Id
SAMEA9908105
INSDC center alias
Novartis Institutes for BioMedical Research
INSDC center name
Novartis Institutes for BioMedical Research
INSDC first public
2021-11-05T12:18:08Z
INSDC last update
2021-11-05T12:18:08Z
INSDC status
public
Submitter Id
E-MTAB-10553_fastq:donor6_S2_6
age
46
broker name
ArrayExpress
cell type
hepatic non-parenchymal cell
common name
human
developmental stage
adult
disease
normal
individual
donor6
organism part
liver
sample name
E-MTAB-10553_fastq:donor6_S2_6
sex
female