strain
not applicable
isolate
not applicable
breed
not applicable
cultivar
not applicable
ecotype
not applicable
age
not applicable
dev_stage
adult
sex
pooled male and female
tissue
head
biomaterial_provider
Hoffmann Lab, School of BioSciences, University of Melbourne, 30 Flemington Rd, Parkville 3010 Victoria, Australia
breeding_history
The offspring of 60 field-collected females were pooled and mass-bred for two generations in the laboratory to a population size of approx. 5000 individuals (generation 0). These 5000 virgin females and males were separated by sex under light CO2 anesthesia and held in separate vials according to sex, at a density of 25 individuals per vial. The selection experiments for desiccation resistance were done separately for both sexes at 4Ñ8 days post eclosion. Flies were desiccated in groups of 25 in glass vials topped with gauze in sealed glass tanks containing silica desiccant (relative humidity < 10%) at 25¡C until approximately 90% of the flies had died, noting the time point at which this occurred (LT90). The surviving 10% of flies were collected and randomly allocated into five replicate lines comprising 100Ñ110 flies of each sex (200Ñ210 in total) per replicate. For each of the following selected generations, 1000 females and 1000 males per line were stressed and the 10% most desiccation-resistant adults (100Ñ110 of each sex, 200Ñ210 in total) were kept as parents for the next generation. Flies were selected for desiccation resistance every generation until generation eight, and every second generation thereafter until generation 21, meaning a total of 13 rounds of selection were performed. This BioSample is a pool of 25 females and 25 males from Replicate 4 after the 13 generations of selection.
breeding_method
All cultures were held at constant 19¡C, under 12 : 12 h light : dark cycle in 250 ml bottles containing laboratory medium composed of dextrose (7.5% w/v), cornmeal (7.3% w/v), inactive yeast (3.5% w/v), soy flour (2% w/v), agar (0.6% w/v), 4-methyl 4-hydroxybenzoate (1.6%) and acid mix (1.4% 10:1 proprionic acid:orthophosphoric acid). The experimental flies were reared under controlled density conditions by removing parents from the bottles after 48 h of oviposition.
collected_by
Sandra Hangartner, sangartner@hotmail.com
geo_loc_name
Australia: Templestowe, Melbourne, Victoria
sample_type
pool of 25 male and 25 female heads (per experiment)
BioSampleModel
Model organism or animal