Protocols: Ears were split into dorsal and ventral sides, floated on Liberase and DNAse in PBS at 37°C for 1 hour, epidermis purified and then digested for a further 15 minutes in Liberase and DNAse in PBS at 37°C for 15 minutes. Epidermal tissue was then processed on a GentleMACS or OctoMACS tissue dissociation with program h_skin_01, and then washed in FACS buffer. For Experiment 0118, mice were untreated. For Experiment 055, mice were treated on the right topical and dorsal surface of the ear with 10 nmol TPA at t=24h prior to sacrifice. TPA was dissolved in EtOH. For Experiment 0124, mice were transduced at E9.5 by in utero injection of lentivirus encoding IL13Ra1-shRNA + tGFP. Cells were FACS sorted in 384-well plates and lysed into 3 ul of Smart-seq2/3 lysis buffer. The lysate was used for library preparation without nucleic acid extraction. Smart-seq3 library preparations were performed as published: https://www.protocols.io/view/smart-seq3-protocol-bcq4ivywLibrary / Hagemann-Jensen et al., 2020.
ENA first public
2022-01-01
ENA last update
2022-01-01
External Id
SAMEA8763252
INSDC center alias
KAROLINSKA INSTITUTE
INSDC center name
KAROLINSKA INSTITUTE
INSDC first public
2022-01-01T00:12:50Z
INSDC last update
2022-01-01T00:12:50Z
INSDC status
public
Submitter Id
E-MTAB-10441:Sample 1
age
12
broker name
ArrayExpress
cell type
epithelial cell
common name
house mouse
developmental stage
adult
genotype
mix of wildtype genotype and Tcrd-GDL homozygous
individual
pool of six mice
organism part
skin epidermis
sample name
E-MTAB-10441:Sample 1
sex
female
strain
C57BL6/N, Tcrd-GDL