Protocols: D. dadantii cultures were grown in M63 medium supplemented with 0.2% glucose and 0.2% PGA, until the early exponential phase (A600nm = 0.2, condition 1), or the early stationary phase (A600nm = 1.8, condition 2). RNAs were extracted using a frozen acid-phenol method, as previously described (Hommais et al. 2008), and treated successively with Roche and Biolabs DNases. Two samples were prepared: 50 µg of RNAs from each condition were pulled into one sample (sample 1), whereas the other one contained 100 µg of RNAs from condition 2 (sample 2). Both samples were then supplied to Vertis Biotechnologie AG for Nanopore native RNA-seq: total RNA preparations were first examined by capillary electrophoresis. For sample 1, ribosomal RNA molecules were depleted using an in-house developed protocol (recovery rate = 84%), whereas no ribodepletion was performed for sample 2. 3' ends of RNA were then poly(A)-tailed using poly(A) polymerase, and the Direct RNA sequencing kit (SQK-RNA002) was used to prepare the library for 1D sequencing on the Oxford Nanopore sequencing device.
ENA first public
2022-01-01
ENA last update
2022-01-01
External Id
SAMEA8756996
INSDC center alias
Universite de Lyon, INSA-Lyon, Universite Claude Bernard Lyon 1, CNRS, UMR5240 MAP, F-69622, France
INSDC center name
Universite de Lyon, INSA-Lyon, Universite Claude Bernard Lyon 1, CNRS, UMR5240 MAP, F-69622, France
INSDC first public
2022-01-01T00:12:50Z
INSDC last update
2022-01-01T00:12:50Z
INSDC status
public
Submitter Id
E-MTAB-10482:expo_stat_G_PGA_ribodepletion
broker name
ArrayExpress
developmental stage
Exponential + stationary phases
genotype
wild type genotype
growth condition
M63+G(0.2%)+PGA(0.2%)
organism part
whole organism
protocol
ribodepletion
sample name
E-MTAB-10482:expo_stat_G_PGA_ribodepletion