Cells were FACS separated. DNA was extracted from the sub-populations and bar-codes for each sub-population were appended to the CDR heavy chains and amplified for Hi-seq sequencing.
strain
EBY100
isolate
pRA11C
host
None
isolation_source
Synthetic
collection_date
2015-08-27
geo_loc_name
USA: Cold Spring Harbor
sample_type
cell culture SNP sequencing
altitude
0 m
biomaterial_provider
Justin Kinney at 1 Bungtown Road Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
collected_by
Rhys Adams
culture_collection
missing
depth
not applicable
env_biome
not applicable
genotype
pRA11
host_tissue_sampled
not applicable
identified_by
Rhys Adams
lab_host
not applicable
lat_lon
40.858312 N 73.465636 W
mating_type
alpha
passage_history
2, FACS
samp_size
5 ml
serotype
pRA11C
serovar
pRA11C
specimen_voucher
missing
temp
4 C
BioSampleModel
Microbe, viral or environmental