Protocols: Sea cucumbers were anesthetized by immersion in MgCl2 (5%) in sea water and tissues were dissected out (tentacles, nerve ring, and radial nerve) and immediately frozen on dry ice and kept at −80 °C for RNA extraction. Total RNA was extracted from 40-45 mg of tissue using the E.Z.N.A. Total RNA Kit I (Omega Bio-Tek, USA) according to the manufacturer's instructions. Samples were treated with RNase-free DNase I (Omega Bio-Tek) to remove possible genomic DNA contamination. The integrity and quality of the extracted RNA was assessed by gel electrophoresis on a 0.8 % agarose TBE gel. The RNA purity and quantity were determined on a NanoDrop One (Thermofisher, Spain). Sequencing library preparation and sequencing was conducted by Admera Health (New Jersey, USA) using a Illumina TrueSeq Stranded mRNA with Poly-A selection library kit (100- 500 ng RNA input)
ENA first public
2022-01-01
ENA last update
2022-01-01
External Id
SAMEA8572161
INSDC center alias
Centro de Ciencias do Mar, Universidade do Algarve
INSDC center name
Centro de Ciencias do Mar, Universidade do Algarve
INSDC first public
2022-01-01T00:12:48Z
INSDC last update
2022-01-01T00:12:48Z
INSDC status
public
Submitter Id
E-MTAB-10369:19211R-02-104
broker name
ArrayExpress
developmental stage
adult
individual
M4R_6RN_19
organism part
radial nerve
sample name
E-MTAB-10369:19211R-02-104
sex
male
stimulus
conditioned water