Protocols: Livers were harvested at ZT18 and immediately frozen before storage at -80. Samples were fixed in 4% formaldehyde for 10 minutes immediately upon thawing, before isolation of nuclei and immunoprecipitation specific histone-DNA complexes. Nucleic acid was extracted by phenol-chloroform and fragmented by sonication. ChIP-seq libraries were generated from the ChIP- and Input-DNA using a custom Illumina library type on an automated system (Apollo 342, Wafergen Biosystems/Takara).
ENA first public
2022-01-01
ENA last update
2022-01-01
External Id
SAMEA11319748
INSDC center alias
OXFORD
INSDC center name
University of Oxford
INSDC first public
2022-01-01T00:13:22Z
INSDC last update
2022-01-01T00:13:22Z
INSDC status
public
Submitter Id
E-MTAB-11203:S5_H3K4me3
age
10 to 14
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
wild type genotype
individual
5
organism part
liver
sample name
E-MTAB-11203:S5_H3K4me3
strain
C57BL/6 x 129 mouse strain