Protocols: Male NOD SCID Mice were implanted with PKH26 labelled U87MG expressing GFP tag. Post 3 weeks, mice were infused with Hoechst 33342 5 min prior to sacrifice. Tumors were isolated post euthanization, single cell dissociated and FACS sorted for perivascular cells (Hoechst+) and divided into two fractions, PKH26+ (dubbed as H+P+) or PKH26- (dubbed as H+P-). RNAs were isolated from the respective sorted fractions (H+P+ and H+P-) using RNeasy mini kit Libraries were prepared and sequenced using Illumina directional RNA sequencing protocol (Hi-Seq).
ENA first public
2022-01-01
ENA last update
2022-01-01
External Id
SAMEA7340288
INSDC center alias
Hebrew University
INSDC center name
Hebrew University
INSDC first public
2022-01-01T00:12:32Z
INSDC last update
2022-01-01T00:12:32Z
INSDC status
public
Submitter Id
E-MTAB-9607:Sample 4
broker name
ArrayExpress
cell line
U-87 MG
common name
human
developmental stage
adult
disease
glioblastoma multiforme
organism part
brain
phenotype
Non label retaining perivascular cells (H+P-)
sample name
E-MTAB-9607:Sample 4
xenograft
Human tumor in mouse