Protocols: Livers were harvested at ZT18 and immediately frozen before storage at -80. Samples were fixed in 4% formaldehyde for 10 minutes immediately upon thawing, before isolation of nuclei and immunoprecipitation specific histone-DNA complexes. Nucleic acid was extracted by phenol-chloroform and fragmented by sonication. ChIP-seq libraries were generated from the ChIP- and Input-DNA using a custom Illumina library type on an automated system (Apollo 342, Wafergen Biosystems/Takara).
ENA first public
2022-01-01
ENA last update
2022-01-01
External Id
SAMEA11319736
INSDC center alias
OXFORD
INSDC center name
University of Oxford
INSDC first public
2022-01-01T00:13:22Z
INSDC last update
2022-01-01T00:13:22Z
INSDC status
public
Submitter Id
E-MTAB-11203:Input
age
10 to 14
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
mixed MLL3 delta mutation and wild type genotype
individual
mixed pool of all mice
organism part
liver
sample name
E-MTAB-11203:Input
sex
mixed
strain
C57BL/6 x 129 mouse strain