Protocols: THP-1 cells were cultured with 5 uM compound 81 or 0.5% DMSO. 5 h later the cells were lysed in Trizol for RNA extraction. Total RNA was extracted from Trizol using the miRNeasy Mini Kit (Qiagen, 217004) according to the manufacturer's instructions. Biological triplicates were obtained for each genotype. 500ng of each sample's total RNA was then prepared into barcoded mRNA libraries, according to manufacturer's instructions, using the Truseq Stranded mRNA Library Prep Kit (Illumina, RS-122-2103).
ENA first public
2021-05-21
ENA last update
2021-05-21
External Id
SAMEA8399496
INSDC center alias
Moores Cancer Center, University of California San Diego, La Jolla 92093, United States
INSDC center name
Moores Cancer Center, University of California San Diego, La Jolla 92093, United States
INSDC first public
2021-05-21T20:29:52Z
INSDC last update
2021-05-21T20:29:52Z
INSDC status
public
Submitter Id
E-MTAB-10269:Sample 2 Vehicle
broker name
ArrayExpress
cell line
THP-1
cell type
monocyte
common name
human
disease
acute monocytic leukemia
organism part
blood
sample name
E-MTAB-10269:Sample 2 Vehicle