Microplankton diatoms were collected from station Environment Canada (EC) 341 located in the west-central basin by vertical net tow using a 154 micron mesh size plankton net deployed to 10 to15 meters depth, following which the diatoms were concentrated by gentle filtration on 20 micron pore size polycarbonate membranes and immediately frozen in liquid nitrogen. Total RNA was extracted from the Lake Erie seston using the RNeasy kit ,Qiagen, MA, USA. The RNA sample was sent to the Georgia Genomics Facility (Athens, GA, USA) to generate a cDNA library followed by 454 sequencing using a Genome Sequencer (GS) FLX Titanium instrument (454 Life Sciences, Branford, CT, USA) employing a standard protocol.
isolation_source
lake erie Epilimnion
collection_date
2009-02-17
geo_loc_name
Canada: Lake Erie EC341
lat_lon
41.79833 N 82.29160000 W
rel_to_oxygen
aerobe
samp_collect_device
plankton net tow
samp_mat_process
frozen stored at -80
samp_size
5g
source_material_id
EC_341 Feb. 2009
BioSampleModel
Metagenome or environmental