Protocols: PBMCs separation was performed by using the Ficoll-Paque (Ficoll Plaque PLUS - GE Healthcare Life Sciences, Piscataway, USA) and the RNA was extracted using the TRIzol reagent (TRIzol Reagent, Invitrogen Life Tecnologies, Carlsband, CA, USA), according to the instructions provided by the manufacturer. For mRNA extraction, each whole blood sample (approx. 10 ml) was processed within 2 hours of collection and the mRNA was stored at -80 °C. One mkwhole blood samples were processed for each patient Indexed libraries were prepared from 1 µg/ea purified RNA with TruSeq Stranded mRNA (Illumina) Library Prep Kit, according to the manufacturer's instructions. Libraries were quantified using the Agilent 2100 Bioanalyzer (Agilent Technologies) and pooled such that each index-tagged sample was present in equimolar amounts, with final concentration of the pooled samples of 2 nM.
ENA first public
2022-01-01
ENA last update
2022-01-01
External Id
SAMEA7745387
INSDC center alias
Genomix4Life SRL
INSDC center name
Genomix4Life SRL
INSDC first public
2022-01-01T00:12:38Z
INSDC last update
2022-01-01T00:12:38Z
INSDC status
public
Submitter Id
E-MTAB-9950:AL11033
broker name
ArrayExpress
cell type
mononuclear cell
common name
human
disease
Parkinson's disease
organism part
blood
sample name
E-MTAB-9950:AL11033
sex
male