Protocols: Paneth cells were isolated by serial dissociation of epithelial cells from mouse small intestine, followed by filtration yielding small intestine crypts. Crypts were then enzymatically dissociated and single cell suspension was stained with CD45, EpCAM and CD24 antibodies for FACS-sorting. Paneth cells were sorted as Singlets/Live/EpCAM+ CD45-/CD24-intermediate SSC-High. Ileum tissue was dissected, cut open longitudinally and immediately snap-frozen in RNA-later on dry ice and stored at -80°C for less then a month. Mice were euthanized by cervical dislocation and their intestine harvested for downstream isolation. RNA from Paneth cells was isolated by Rneasy micro kit plus (QIAGEN) straight after sorting. RNA from ileum tissue was isolated by Rneasy mini kit plus (QIAGEN). Libraries were prepared with a SMARTer® Stranded Total RNA-Seq - Pico Input Mammalian library preparation kit v2 (Takara).
ENA first public
2022-01-01
ENA last update
2022-01-01
External Id
SAMEA7871773
INSDC center alias
Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of the ASCR, v. v. i.
INSDC center name
Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of the ASCR, v. v. i.
INSDC first public
2022-01-01T00:12:39Z
INSDC last update
2022-01-01T00:12:39Z
INSDC status
public
Submitter Id
E-MTAB-10038:IL17Ra_delta_PC_ILE_5
age
6
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
Defa6-iCre x Il17ra-flox
individual
13
organism part
ileum
phenotype
Cre positive
sample name
E-MTAB-10038:IL17Ra_delta_PC_ILE_5
sex
female
strain
C57BL/6J