strain
not applicable
isolate
not applicable
breed
not applicable
cultivar
not applicable
ecotype
not applicable
age
not applicable
dev_stage
adult
sex
pooled male and female
tissue
head
biomaterial_provider
Hoffmann Lab, School of BioSciences, University of Melbourne, 30 Flemington Rd, Parkville 3010 Victoria, Australia
breeding_history
The offspring of 60 field-collected females were pooled and mass-bred for two generations in the laboratory to a size of approx. 5000 individuals (generation 0). These 5000 virgin females and males were separated by sex under light CO2 anesthesia and held in separate vials according to sex, at a density of 25 individuals per vial. A random subset of flies were randomly allocated into five replicate lines comprising 100Ñ110 flies of each sex (200Ñ210 in total) per replicate. For each of the following generations, 1000 females and 1000 males per line were retained and a randomly-chosen 10% of adults (100Ñ110 of each sex, 200Ñ210 in total) were kept as parents for the next generation. Population sizes were maintained in this way every generation until generation eight, and every second generation thereafter until generation 21. In the alternate generations, populations were maintained at approx. 2000 individuals. This BioSample is a pool of 25 females and 25 males from Replicate 4 after the 2+21 generations of culture (the same timepoint as the selected lines after 13 generations of selection).
breeding_method
All cultures were held at constant 19¡C, under 12 : 12 h light : dark cycle in 250 ml bottles containing laboratory medium composed of dextrose (7.5% w/v), cornmeal (7.3% w/v), inactive yeast (3.5% w/v), soy flour (2% w/v), agar (0.6% w/v), 4-methyl 4-hydroxybenzoate (1.6%) and acid mix (1.4% 10:1 proprionic acid:orthophosphoric acid). The experimental flies were reared under controlled density conditions by removing parents from the bottles after 48 h of oviposition.
collected_by
Sandra Hangartner, sangartner@hotmail.com
geo_loc_name
Australia: Templestowe, Melbourne, Victoria
sample_type
pool of 25 male and 25 female heads (per experiment)
BioSampleModel
Model organism or animal