Protocols: MCF7 cells were grown in DMEM supplemented with 10% FBS. For ChIP-seq, MCF7 cells were crosslinked with 1% paraformaldehyde for 10 minutes at room temperature with gentle rotation, and then quenched by 0.125 M glycine solution. After washing, nuclei were sonicated and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-sequencing libraries were prepared with NEB Next Ultra II DNA Library preparation kit.
ENA first public
2022-01-01
ENA last update
2022-01-01
External Id
SAMEA8477067
INSDC center alias
Center for DNA Fingerprinting and Diagnostics (CDFD)
INSDC center name
Center for DNA Fingerprinting and Diagnostics (CDFD)
INSDC first public
2022-01-01T00:12:46Z
INSDC last update
2022-01-01T00:12:46Z
INSDC status
public
Submitter Id
E-MTAB-10295:WT Input replicate 1
broker name
ArrayExpress
cell line
MCF-7
common name
human
disease
breast adenocarcinoma
genotype
wild type genotype
organism part
mammary gland
sample name
E-MTAB-10295:WT Input replicate 1