Protocols: WT and isw1Δ exponentially growing cells were treated with 1 μg/mL tunicamycin for 2h, washed and resuspended in Tm free medium. Samples were collected at t= -2h (Untreated), t = 0 (Tm 2 H), and t = 3 H (Wash 3 H) for analysis by RNA seq RNA was isolated from 10 OD of exponentially growing cells washed in MilliQ water and snap frozen. RNA was extracted with the Nucleospin RNA II Kit (Macherey-Nagel) mRNA sequencing libraries were prepared from polyA+ RNAs purified from 1 ug of total RNA using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. A 150 bp read sequencing was Illumina sequenced with multiplexing. A mean of 17.7 ± 2.4 million passing Illumina quality filter reads was obtained for each of the samples. Library preparation was performed by Novogene (Novogene Co., Ltd).
ENA first public
2022-01-01
ENA last update
2022-01-01
External Id
SAMEA8800220
INSDC center alias
INSERM
INSDC center name
INSERM
INSDC first public
2022-01-01T00:12:51Z
INSDC last update
2022-01-01T00:12:51Z
INSDC status
public
Submitter Id
E-MTAB-10511:Sample 1
broker name
ArrayExpress
common name
baker's yeast
genotype
wild type genotype
sample name
E-MTAB-10511:Sample 1
strain
W303