V1V2.RNA.215.A
ENA first public
2021-04-17
ENA last update
2021-04-04
External Id
SAMEA8514429
INSDC center alias
UNIVERSITY OF CALIFORNIA - SAN DIEGO
INSDC center name
UNIVERSITY OF CALIFORNIA - SAN DIEGO
INSDC first public
2021-04-17T00:26:22Z
INSDC last update
2021-04-04T04:06:32Z
INSDC status
public
Submitter Id
V1V2.RNA.215.A
adapters
TruSeq12
amount or size of sample collected
2
collection date
2012-08-13
common name
marine_sediment_metagenome
environment (biome)
marine_abyssal_zone_biome
environment (feature)
marine_oligotrophic_desert
environment (material)
mud
geographic location (country and/or sea)
Pacific Ocean
geographic location (depth)
5298
geographic location (elevation)
0
geographic location (latitude)
42.48267
geographic location (longitude)
153.99867
geographic location (region and locality)
North_Pacific:KuramBio
investigation type
mimarks-survey
library construction method
TruSeq_Nano_PCR_free_Library_Preparation_Kit_single-end
library reads sequenced
3720707
library screening strategy
normalized
library size
40
library vector
TruSeq
multiplex identifiers
F04-R:_{GTGACGTAGCTTGTCTCAAAGATTAAGCC}_R22mod-P:_{GAGCACGTCCTGCTGCCTTCCTTRGA}
nucleic acid amplification
https://github.com/FranckLejzerowicz/protocols
nucleic acid extraction
https://www.qiagen.com/us/cmp/ecom/powersoilkits?COECM_MOBIO_Branded_1707_SEA_PowerSoil__&kwid=%2Bpower%20%2Bsoil%20%2Brna
pcr conditions
initial_denaturation:95_5;_denaturation:_94_0.5;_annealing:57_0.5;_elongation:_72_1;_35_cycles;_final_elongation:72_2
pcr primers
FWD:_{GCTTGTCTCAAAGATTAAGCC}_REV:_{CCTGCTGCCTTCCTTRGA}
project name
DSMP
sample collection device or method
multiple_corer
sample material processing
lifeguard_preservation_solution
sample name
V1V2.RNA.215.A
sample volume or weight for DNA extraction
2
sediment environmental package
sediment
sequencing method
Illumina
source material identifiers
DSMP_215
target gene
18S_rRNA
target subfragment
V1V2