V1V2.DNA.249.B
ENA first public
2021-04-17
ENA last update
2021-04-04
External Id
SAMEA8513877
INSDC center alias
UNIVERSITY OF CALIFORNIA - SAN DIEGO
INSDC center name
UNIVERSITY OF CALIFORNIA - SAN DIEGO
INSDC first public
2021-04-17T00:26:20Z
INSDC last update
2021-04-04T04:06:14Z
INSDC status
public
Submitter Id
V1V2.DNA.249.B
adapters
TruSeq7
amount or size of sample collected
2
collection date
2012-02-19
common name
marine_sediment_metagenome
environment (biome)
marine_abyssal_zone_biome
environment (feature)
marine_oligotrophic_desert
environment (material)
mud
geographic location (country and/or sea)
Atlantic Ocean
geographic location (depth)
4113
geographic location (elevation)
0
geographic location (latitude)
-51.2661667
geographic location (longitude)
-12.6156667
geographic location (region and locality)
Southern_Ocean:SYSTCOII
investigation type
mimarks-survey
library construction method
TruSeq_Nano_PCR_free_Library_Preparation_Kit_single-end
library reads sequenced
7399097
library screening strategy
normalized
library size
42
library vector
TruSeq
multiplex identifiers
F04-Y:_{TCATGACAGCTTGTCTCAAAGATTAAGCC}_R22mod-Q:_{GAGCGTGACCTGCTGCCTTCCTTRGA}
nucleic acid amplification
https://github.com/FranckLejzerowicz/protocols
nucleic acid extraction
https://www.qiagen.com/us/cmp/ecom/powersoilkits?COECM_MOBIO_Branded_1707_SEA_PowerSoil__&kwid=%2Bpower%20%2Bsoil%20%2Brna
pcr conditions
initial_denaturation:95_5;_denaturation:_94_0.5;_annealing:57_0.5;_elongation:_72_1;_35_cycles;_final_elongation:72_2
pcr primers
FWD:_{GCTTGTCTCAAAGATTAAGCC}_REV:_{CCTGCTGCCTTCCTTRGA}
project name
DSMP
sample collection device or method
multiple_corer
sample material processing
Freezer_-80C
sample name
V1V2.DNA.249.B
sample volume or weight for DNA extraction
2
sediment environmental package
sediment
sequencing method
Illumina
source material identifiers
DSMP_249
target gene
18S_rRNA
target subfragment
V1V2