PN0179_00139_S138
ENA-FIRST-PUBLIC
2022-07-08
ENA-LAST-UPDATE
2022-07-08
External Id
SAMEA7049335
INSDC center alias
VETERINARY MEDICINE UNIVERSITY
INSDC center name
VETERINARY MEDICINE UNIVERSITY
INSDC first public
2022-07-08T00:17:59Z
INSDC last update
2022-07-08T00:17:59Z
INSDC status
public
Submitter Id
Rumen fluid#8
adapters
Illumina adapters
collection date
2019
environment (biome)
rumen
environment (feature)
GIT
environment (material)
ruminal fluid
geographic location (country and/or sea)
Portugal
geographic location (latitude)
39.200829
geographic location (longitude)
-8.739634
geographic location (region and locality)
Santarém
host age
6 months
host color
white
host common name
sheep
host diet
O
host sex
male
host-associated environmental package
host-associated
investigation type
metagenome
nucleic acid amplification
PCR
nucleic acid extraction
Fast DNA SPIN Kit for Soil
pcr conditions
95ºC for 3 minutes, 20 cycles at 95ºC for 30 seconds, 55ºC for 30 seconds, 72ºC for 30 seconds and then 72ºC for 5 minutes.
pcr primers
515F and 806R
project name
CIISA18-NannOmega3
sample name
Rumen fluid#8
sample storage temperature
-80
sample volume or weight for DNA extraction
0.05
sequencing method
Illumina Miseq
target gene
16S: V4- 250 bp