PN0179_00129_S128
ENA-FIRST-PUBLIC
2022-07-08
ENA-LAST-UPDATE
2022-07-08
External Id
SAMEA7049384
INSDC center alias
VETERINARY MEDICINE UNIVERSITY
INSDC center name
VETERINARY MEDICINE UNIVERSITY
INSDC first public
2022-07-08T00:17:59Z
INSDC last update
2022-07-08T00:17:59Z
INSDC status
public
Submitter Id
Negative control#1
adapters
Illumina adapters
collection date
2020
environment (biome)
Lab environment
environment (feature)
liquid
environment (material)
PBS
geographic location (country and/or sea)
United Kingdom
geographic location (latitude)
54.579277
geographic location (longitude)
-5.937485
geographic location (region and locality)
Belfast
host age
0
host-associated environmental package
host-associated
investigation type
metagenome
nucleic acid amplification
PCR
nucleic acid extraction
Fast DNA SPIN Kit for Soil
pcr conditions
95ºC for 3 minutes, 20 cycles at 95ºC for 30 seconds, 55ºC for 30 seconds, 72ºC for 30 seconds and then 72ºC for 5 minutes.
pcr primers
515F and 806R
project name
CIISA18-NannOmega3
sample name
Negative control#1
sample storage temperature
20
sample volume or weight for DNA extraction
5 μL
sequencing method
Illumina Miseq
target gene
16S: V4- 250 bp