Premise plumbing water, Rome building 1, hot recirc water, autumn 2017
ENA-FIRST-PUBLIC
2022-08-30
ENA-LAST-UPDATE
2022-08-30
External Id
SAMEA14391714
INSDC center alias
Scottish Microbiology Reference Laboratories
INSDC center name
scottish microbiology reference laboratories
INSDC first public
2022-08-30T00:16:06Z
INSDC last update
2022-08-30T00:16:06Z
INSDC status
public
Submitter Id
1830A
building ID
R_B1
building type
Hospital
collection date
2017-10
compartment ID
R_B1_HRW
culturable Legionella
35000
environment (biome)
ENVO:00002030
environment (material)
ENVO:00003064
geographic location (country and/or sea)
Italy
geographic location (depth)
0
geographic location (latitude)
41.9
geographic location (longitude)
12.5
geographic location (region and locality)
Rome
investigation type
mimarks-survey
nucleic acid extraction
PowerWater DNA isolation kit (Qiagen)
pH
7.4
pcr primers
V3V4-F:TACGGGAGGCAGCAG, V3V4-R:CCAGGGTATCTAATCC
plumbing component
hot recirculating water
project name
Bacterial communities and culturable Legionella in premise plumbing of four European cities
sample name
1830A
sample volume or weight for DNA extraction
1000
sequencing method
Illumina MiSeq v3 PE300
target gene
16S rRNA
target subfragment
V3-V4
temperature
47.4
water environmental package
water