Control
ENA-FIRST-PUBLIC
2022-07-09
ENA-LAST-UPDATE
2022-07-09
External Id
SAMEA7050491
INSDC center alias
QUEEN'S UNIVERSITY BELFAST
INSDC center name
QUEEN'S UNIVERSITY BELFAST
INSDC first public
2022-07-09T00:20:14Z
INSDC last update
2022-07-09T00:20:14Z
INSDC status
public
Submitter Id
Positive control
adapters
Illumina adapters
collection date
2020
common name
Positive control
environment (biome)
lab
environment (feature)
liquid
environment (material)
Commercial mock community
geographic location (country and/or sea)
United Kingdom
geographic location (latitude)
54.579.058
geographic location (longitude)
-5.936.998
geographic location (region and locality)
Belfast
host age
0
host-associated environmental package
host-associated
investigation type
bacteria_archaea
nucleic acid amplification
PCR
nucleic acid extraction
Fast DNA SPIN Kit for Soil
pcr conditions
95�C for 3 minutes, 20 cycles at 95�C for 30 seconds, 55�C for 30 seconds, 72�C for 30 seconds and then 72�C for 5 minutes.
pcr primers
515F and 806R
project name
EasyCare_2018
sample name
Positive control
sample storage temperature
-80
sample volume or weight for DNA extraction
75uL
sequencing method
Illumina MiSeq
target gene
16S: V4- 250 bp