Protocols: Plant leaves were harvested 48 hours after treatment at 10.30 am. For one biological replicate, leaves of 25 plants were pooled and immediately frozen in liquid N2. The plant material was homogenized in liquid N2 with mortar and pestle. Plants were grown for three weeks in a growth chamber (light/dark cycle: 10/14 h at 20/16 °C, 80/65 % relative humidity, light at 130 µmol m^(-2) s^(-1)) on a peatmoss-base (Floragard Multiplication substrate, Germany) and quartz sand substrate mixture (8:1). Three-week-old plants were sprayed with water (as control) or Benzothiadiazole (BTH, BION®, Ciba-Geigy, Germany), a chemical analog of salicylic acid. In both cases the spraying mixture contained 0.01 % Silwet L-77 (Lehle Seeds, USA), which supports entering of BTH into the leaves. After 1 h, plants were covered with a plastic dome. Total RNA extraction was carried out with the innuPREP RNA KIT (Analytik Jena, Germany) according to the manufacturer's instructions. Transcriptome library construction was performed by BGI Tech Solutions Co., Ltd. (BGI-Tech, Hongkong).
ENA-FIRST-PUBLIC
2021-03-31T04:07:15Z
ENA-LAST-UPDATE
2020-07-03T21:43:45Z
External Id
SAMEA7039578
INSDC center name
Institute of Biochemical Plant Pathology Helmholtz Zentrum Munchen Deutsches Forschungszentrum fur Gesundheit und Umwelt (GmbH)
INSDC first public
2021-03-31T04:07:15Z
INSDC last update
2020-07-03T21:43:45Z
INSDC status
public
Submitter Id
E-MTAB-9300:Sample 31
age
3
broker name
ArrayExpress
common name
thale cress
developmental stage
rosette growth stage
ecotype
Col-0
genotype
ugt76b1; ugt74f1; ugt74f2
organism part
rosette leaf
sample name
E-MTAB-9300:Sample 31
scientific_name
Arabidopsis thaliana