Protocols: Human IEO were generated from mucosal biopsies by isolation of intestinal crypts and culturing in Matrigel® (Corning) In vitro differentiation of human IEO was performed by culturing IEO in standard growth medium for four days followed by removal of wnt agonists (using a modified differentiation medium) At the end of each experiment, human IEO were harvested and both DNA and RNA were extracted using the AllPrep DNA/RNA mini kit (Qiagen, Hilden, Germany). RNA was prepared with the Truseq mRNA library preparation (Illumina, San Diego, CA, USA)
ENA-FIRST-PUBLIC
2022-08-31
ENA-LAST-UPDATE
2022-08-31
External Id
SAMEA13410483
INSDC center alias
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK
INSDC center name
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK
INSDC first public
2022-08-31T00:16:41Z
INSDC last update
2022-08-31T00:16:41Z
INSDC status
public
Submitter Id
E-MTAB-11548:T383_SC_p9_D
age
3
broker name
ArrayExpress
cell_line
organoid
cell_type
intestinal epithelial cell
common name
human
developmental stage
paediatric
differentiation
differentiated
disease
normal
growth condition
organoid culture
individual
T383
organism part
sigmoid colon
passage
9
passage_highlow
high
sample name
E-MTAB-11548:T383_SC_p9_D
sex
female