Protocols: At least 50 seedlings were pooled from three independent petri dishes for RNA extraction. Arabidopsis thaliana seeds were surfaced sterilized and sown in petri dishes with agarified (0.8% Bacto Agar) 0.5x MS medium, containing 1% sucrose and 0.05% MES at pH 5.7. After stratification for 3 days at 4°C, seedlings were grown in a CU-22L growth chamber (Percival) under 21°C and a 12/12 h (day/night) light cycle. For treatment, 7 days-old seedlings were subjected to changes in temperature only during the light period. Long-term heat consisted of an entrainment treatment of 5 heat cycles (day 37C, night 21C, 12/12 h; age at harvest 12 days). Controls were kept at 21C (age at harvest 12 days). Total RNA was isolated from biologically duplicated samples using the the Plant-RNAeasy kit (Invitrogen), following provider's instructions. Libraries were prepared with the corresponding Illumina library preparation kit, following provider's instructions.
ENA-FIRST-PUBLIC
2022-08-31
ENA-LAST-UPDATE
2022-08-31
External Id
SAMEA10083246
INSDC center alias
IFEVA (CONICET-UBA), Facultad de Agronomia (FAUBA), Universidad de Buenos Aires (UBA)
INSDC center name
IFEVA (CONICET-UBA), Facultad de Agronomia (FAUBA), Universidad de Buenos Aires (UBA)
INSDC first public
2022-08-31T00:12:58Z
INSDC last update
2022-08-31T00:12:58Z
INSDC status
public
Submitter Id
E-MTAB-10925:Sample_21
age
12
broker name
ArrayExpress
common name
thale cress
developmental stage
seedling development stage
environmental stress
warm/hot temperature regimen
genotype
wild type genotype
organism part
whole organism
sample name
E-MTAB-10925:Sample_21
ecotype
Col-0