Protocols: W12 cells were cultured in a monolayer to restrict cell differentiation and maintain the phenotype of the basal epithelial. Cells were collected at the lowest possible passage after cloning (p3 to p8) to minimise any effects of genomic instability caused byderegulated HPV16 oncogenes. Cells were lysed on ice for 30 minutes in 50 ml freshly prepared icecold lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% Igepal CA-630, one protease inhibitor cocktail tablet (Roche complete, EDTA-free)). Total RNA was isolated and subjected to Ribo-Zero rRNA depletion and DNAse treatment. cDNA libraries were prepared from the ribo-depleted RNA with the TruSeq™ RNA and DNA Sample Prep Kit (Illumina)
ENA first public
2021-03-31
ENA last update
2021-06-18
External Id
SAMEA8139054
INSDC center alias
EMBL-EBI
INSDC center name
EMBL-EBI
INSDC first public
2021-03-31T04:07:30Z
INSDC last update
2021-06-18T16:17:04Z
INSDC status
public
Submitter Id
E-MTAB-10155:W12 clone H rep I
broker name
ArrayExpress
cell line
W12
cell type
epithelial cell
common name
human
disease
squamous cell neoplasm
organism part
cervix epithelium
sample name
E-MTAB-10155:W12 clone H rep I
strain
W12 clone H