Protocols: Clones of strains were cultivated overnight in 15 mL of Delft medium added with 40 mg/l uracil up to an OD600 around two. The Blood & Cell Culture DNA Mini Kit (Qiagen) was used to extract genomic DNA from the overnight yeast culture using the protocol recommended by the manufacturer. DNA libraries were prepared using a Kapa Hyper Prep Library Prep Kit (Roche Molecular Systems).
ENA first public
2021-03-31
ENA last update
2020-12-10
External Id
SAMEA7703201
INSDC center alias
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark,
INSDC center name
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark,
INSDC first public
2021-03-31T17:11:28Z
INSDC last update
2020-12-10T17:17:47Z
INSDC status
public
Submitter Id
E-MTAB-9898:JQ18-2
broker name
ArrayExpress
common name
baker's yeast
genotype
MATa SUC2 MAL2-8c ura3-52 his3-1 ARG3p::KEX2p car2::LoxP-CTC1t-AGC1-tHXT7p-TPIp-ORT1-pYX212t ura3:: LoxP-TEF1p-GDH1-DIT1t XI-3::TDH3p-CgargJ-TDH2t-ADH1t-EcargB-PGK1p-TEF1p-CgargC-FBA1t-TPI1t-CgargD-tHXT7p-TPI1p-EcargA-CYC1t oaz1::LoxP-KanMX-LoxP-pYX212t-SPE3-PGK1p-TEF1p-SPE1-PRM9t-DIT1t-SPE2-TDH3p {pCAS9-HIS}
sample name
E-MTAB-9898:JQ18-2
strain
CEN.PK 113-11C