Protocols: Cryopreserved AML-PDX cells have routinely been frozen as 10 Mio cells in 1mL of freezing medium (80% FBS, 20% DMSO) at -80 °C for archiving purposes. To avoid thawing these samples and thus harming or even destroying them, the frozen cell stocks were first transferred to dry ice under a cell culture hood. Next a sterile 1mm biopsy punch was used to punch the frozen cells in the vial and transfer the extracted cells to one well of a 96 well plate containing 100 µL RLTplus lysis buffer with 1% beta mercaptoethanol. To ensure complete lysis the lysate was mixed and snap frozen on dry ice. prime-seq RNA extraction using magnetic beads (see prime-seq protocol) The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66
ENA first public
2021-03-31
ENA last update
2021-03-03
External Id
SAMEA8172389
INSDC center alias
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC center name
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC first public
2021-03-31T04:07:31Z
INSDC last update
2021-03-03T11:08:12Z
INSDC status
public
Submitter Id
E-MTAB-10175:ATAAAC_AML372
broker name
ArrayExpress
cell line
AML372
cell type
AML blast
common name
human
developmental stage
adult
disease
acute myeloid leukemia
genotype
complex karyotype
organism part
bone marrow
passage
6
passage_bin
Late Passage(>4)
sample barcode
ATAAAC
sample name
E-MTAB-10175:ATAAAC_AML372