TARGET TF-perturbation assay data with ABF3 vector R1
isolate
TARGET TF-perturbation assay data with ABF3 vector R1
ecotype
Col-0
age
15-day-old
geo_loc_name
Chile
tissue
Roots-protoplast
growth_protocol
Arabidopsis were grown hydroponically in 200 mL of medium containing 50 M H3BO3, 1.5 mM CaCl2, 50 M MnSO4, 0.08 M CuSO4, 0.05 M Na2MoO4, 0.625 mM KH2PO4, 0.75 mM MgSO4, 25 M ZnSO4, 5 M KI, 50 M FeSO4, 50 M Na2EDTA, and 0.055 M CoCl2 supplemented with 0.5 mM ammonium succinate as N source for two weeks.
sample_type
root protoplast for TARGET analysis
treatment
to identify direct regulated targets, 35M cycloheximide was added 20min before a 10M dexamethasone treatment
expression_vector
ABF3-RFP
BioSampleModel
Plant