NR-54974 lot 70041987 was produced by infecting Homo sapiens lung adenocarcinoma cells (Calu-3; ATCC® HTB-55™) with severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/South Africa/KRISP-K005325/2020 and incubating in Eagle's Minimum Essential Medium (ATCC® 30-2003™) supplemented with 2% fetal bovine serum (ATCC® 30-2020™) for 5 days at 37°C with 5% CO2. Cell lysate and supernatant was clarified by centrifuging at 1500 × g for 10 minutes at room temperature and vialed (0.1 mL per vial). Then Bioqual conducted a fifth passage using Calu-3 cells.
strain
hCoV-19/South Africa/KRISP-K005325/2020
isolate
Homo sapien
collected_by
BIOQUAL
collection_date
2021-03-06
geo_loc_name
USA: Maryland, Rockville
host
Homo sapiens
host_disease
COVID-19
isolation_source
not appliable
lat_lon
39.0840 N 77.1528 W
culture_collection
BEI<USA>:70041987
passage_history
H(1)VE(2)/C(1)/C(1) (African Health Research Institute/BEI Resources/BIOQUAL); H = H1299 cells overexpressing human ACE2 receptor (H1299-hACE2-E3); VE = Vero E6 cells; C = Calu-3 cells
replicate
biological replicate 1
BioSampleModel
Pathogen.cl