Protocols: In total, 100x106 SK-N-BE(2)-C and Kelly neuroblastoma cells were processed for ChIP-sequencing. Kelly and BE2C neuroblastoma cell lines were obtained from the American Type Culture Collection (BE2C) and DSMZ (Kelly). All cell lines used for the genome-scale CRISPR–Cas9 screen have been previously described7. All cell lines were short tandem repeat (STR) tested for identity. Neuroblastoma cell lines were cultured in RPMI media containing 10% fetal bovine serum and 1% penicillin-streptomycin and validated to be free of Mycoplasma species. The pellets from the samples were prepared by fixing cells in 1% formaldehyde for 15 minutes, and quenching in 1M Tris pH 7.5. Nuclei were prepared using the Sigma Nuclei Isolation Kit (#NUC-101), and sonicated using a Branson Digital Sonifier. ChIP-seq libraries were prepared using the KAPA HyperPrep ChIP library kit following the manufacturer's settings.
ENA-FIRST-PUBLIC
2022-04-14
ENA-LAST-UPDATE
2022-04-14
External Id
SAMEA9253519
INSDC center alias
Ugent
INSDC center name
Ghent University
INSDC first public
2022-04-14T00:23:48Z
INSDC last update
2022-04-14T00:23:48Z
INSDC status
public
Submitter Id
E-MTAB-10620:Sample 1
broker name
ArrayExpress
cell_line
KELLY
cell_type
neuroblast (sensu Vertebrata)
common name
human
disease
neuroblastoma
isolate
not applicable
organism part
brain
sample name
E-MTAB-10620:Sample 1