Protocols: Dissected gastrocnemius muscles of individual rats were immediately snap frozen and stored at −80 °C until use. For total RNA, muscles were pulverized, and 15-20 mg were lysed in 1000μl QIAZol (Qiagen #79306), homogenized for 10 sec at 6000 rpm using the Precyllis (Bertin Instruments #P000669-PR240-A) and proceeded following the manufacturer recommendation. The aqueous phase was transferred to a 2 ml Eppendorf tube. RNA was purified using a customized protocol for the Qiacube for large volume with DNase digest and the RNeasy Micro Kit (Qiagen #74004). RNA purity was determined using the Experion™ Automated Electrophoresis System. TruSeq RNA Sample Prep Kit v2 (Illumina)
ENA first public
2021-06-26
ENA last update
2021-06-26
External Id
SAMEA9211583
INSDC center alias
Novartis Institutes for BioMedical Research
INSDC center name
Novartis Institutes for BioMedical Research
INSDC first public
2021-06-26T00:12:41Z
INSDC last update
2021-06-26T00:12:41Z
INSDC status
public
Submitter Id
E-MTAB-10583:AS_IL_LIB_20140725_VR_A034
age
21
broker name
ArrayExpress
common name
Norway rat
developmental stage
old
genotype
wild type genotype
individual
31
organism part
gastrocnemius
sample name
E-MTAB-10583:AS_IL_LIB_20140725_VR_A034
sex
male
strain
Sprague-Dawley