Protocols: Cryopreserved AML-PDX cells have routinely been frozen as 10 Mio cells in 1mL of freezing medium (80% FBS, 20% DMSO) at -80 °C for archiving purposes. To avoid thawing these samples and thus harming or even destroying them, the frozen cell stocks were first transferred to dry ice under a cell culture hood. Next a sterile 1mm biopsy punch was used to punch the frozen cells in the vial and transfer the extracted cells to one well of a 96 well plate containing 100 µL RLTplus lysis buffer with 1% beta mercaptoethanol. To ensure complete lysis the lysate was mixed and snap frozen on dry ice. prime-seq RNA extraction using magnetic beads (see prime-seq protocol) The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66
ENA first public
2021-03-31
ENA last update
2021-03-03
External Id
SAMEA8172407
INSDC center alias
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC center name
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC first public
2021-03-31T04:07:31Z
INSDC last update
2021-03-03T11:08:13Z
INSDC status
public
Submitter Id
E-MTAB-10175:CCGGAC_AML491
broker name
ArrayExpress
cell line
AML491
cell type
AML blast
common name
human
developmental stage
adult
disease
acute myeloid leukemia
genotype
not favourable nor adverse
organism part
bone marrow
passage
0
passage_bin
Initial Passage(0)
sample barcode
CCGGAC
sample name
E-MTAB-10175:CCGGAC_AML491