Protocols: Human IEO were generated from mucosal biopsies by isolation of intestinal crypts and culturing in Matrigel® (Corning) At the end of each experiment, human IEO were harvested and both DNA and RNA were extracted using the AllPrep DNA/RNA mini kit (Qiagen, Hilden, Germany). RNA was prepared with the Truseq mRNA library preparation (Illumina, San Diego, CA, USA)
ENA-FIRST-PUBLIC
2022-08-31
ENA-LAST-UPDATE
2022-08-31
External Id
SAMEA13410465
INSDC center alias
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK
INSDC center name
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK
INSDC first public
2022-08-31T00:16:41Z
INSDC last update
2022-08-31T00:16:41Z
INSDC status
public
Submitter Id
E-MTAB-11548:T427_TI_p3_UT
age
5
broker name
ArrayExpress
cell_line
organoid
cell_type
intestinal epithelial cell
common name
human
developmental stage
paediatric
differentiation
undifferentiated
disease
normal
growth condition
organoid culture
individual
T427
organism part
terminal ileum
passage
3
passage_highlow
low
sample name
E-MTAB-11548:T427_TI_p3_UT
sex
male