Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate USA/CA_CDC_5574/2020 was isolated from a nasopharyngeal swab on December 29, 2020 in San Diego County, California, USA. NR-54011 lot 70041598 was produced by infecting Cercopithecus aethiops kidney epithelial cells (Vero; ATCC® CCL-81™) with the deposited material and incubating in Eagle's Minimum Essential Medium (ATCC® 30-2003™) supplemented with 2% fetal bovine serum (ATCC® 30-2020™) for 4 days at 37°C with 5% CO2. Bioqual then conducted a fourth passage using Calu-3 cells.
strain
USA/CA_CDC_5574/2020
isolate
Homo sapien
collected_by
BIOQUAL
collection_date
2021-01-29
geo_loc_name
USA: Maryland, Rockville
host
Homo sapiens
host_disease
COVID-19
isolation_source
not appliable
lat_lon
39.0840 N 77.1528 W
culture_collection
BEI<USA>:70041598
passage_history
V(2)/V(1)/C(1) (Centers for Disease Control and Prevention/BEI Resources/BIOQUAL); V = Vero cells C= Calu-3 Cells
replicate
biological replicate 2
BioSampleModel
Pathogen.cl