Protocols: Cells were cultured and differentiated into NPCs as described previously (Geuder et. al. 2021). One colony per sample was harvested and lysed in 100 µl RLT plus lysis buffer containing 1% beta-mercapto Ethanol and frozen at -80 °C. RNA extraction using carboxyl coated beads as described in the prime-seq protocol The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66
ENA first public
2021-03-31
ENA last update
2021-02-25
External Id
SAMEA8137182
INSDC center alias
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC center name
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC first public
2021-03-31T04:07:30Z
INSDC last update
2021-02-25T11:24:22Z
INSDC status
public
Submitter Id
E-MTAB-10133:Human2_iPSC
broker name
ArrayExpress
cell line
1383B2
cell type
induced pluripotent stem cell
common name
human
disease
normal
genotype
wild type genotype
individual
Human2
progenitor cell type
induced pluripotent stem cell
sample barcode
CATCAACTCGAA
sample name
E-MTAB-10133:Human2_iPSC