NR-54009 lot 70041942 was produced by infecting Cercopithecus aethiops kidney epithelial cells with human signaling activation molecule (Vero-hSLAM) with severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/South Africa/KRISP-K005325/2020 and incubating in Eagle's Minimum Essential Medium (ATCC® 30-2003™) supplemented with 2% fetal bovine serum (ATCC® 30-2020™) for 4 days at 37°C with 5% CO2. Cell lysate and supernatant was clarified by centrifuging at 1500 × g for 10 minutes at room temperature. Bioqual then conducted a fourth passage using Calu-3 cells.
strain
hCoV-19/South Africa/KRISP-K005325/2020
isolate
Homo sapien
collected_by
BIOQUAL
collection_date
2021-02-10
geo_loc_name
USA: Maryland, Rockville
host
Homo sapiens
host_disease
COVID-19
isolation_source
not appliable
lat_lon
39.0840 N 77.1528 W
culture_collection
BEI<USA>:70041942
passage_history
H(1)VE(2)/C(1) (African Health Research Institute/BEI Resources/BIOQUAL); H = H1299 cells overexpressing human ACE2 receptor (H1299-hACE2-E3); VE = Vero E6 cells C= Calu-3 Cells
replicate
biological replicate 1
BioSampleModel
Pathogen.cl