Protocols: HiPSCs Derived Cardiomyocytes were exposed to SMG for 48 hr and identical set were kept at 1g post 48 hrs cells were harvested for RNA extraction. RNA isolation was done by using RNAqueous™ Micro Total RNA Isolation Kit AM1931 from (Ambion). Total RNA concentration was measure by UV-Vis spectrophotometer nanodrop2000c (Thermo Fisher). Furthermore, samples were measured on the Fragment Analyzer 5200 (Agilent). In case of gDNA contamination, the DNase I (Thermo Fisher) digest was performed. Then samples were processed for library preparation. Sequencing-ready libraries were produced using a QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (015UG009V0241) following the standard procedures, as described by Lexogen. Indexed library preparation was performed to allow multiplexed sequencing. 250ng mRNA samples were used as input for the library preparation. Finished libraries were quality controlled on a Bioanalyzer device (Agilent), using the HS-DNA assay. Concentration of obtained libraries were quantified using a Qubit dsDNA HS assay (Thermo Fisher). A sequencing-ready pool of indexed libraries were prepared according to these quantifications.
ENA first public
2021-12-05
ENA last update
2021-12-05
External Id
SAMEA11024810
INSDC center alias
Postdoc
INSDC center name
Postdoc
INSDC first public
2021-12-05T00:30:38Z
INSDC last update
2021-12-05T00:30:38Z
INSDC status
public
Submitter Id
E-MTAB-11159:Sample41_1g_1
broker name
ArrayExpress
cell type
derived cardiomyocyte
common name
human
progenitor cell type
induced pluripotent stem cell
sample name
E-MTAB-11159:Sample41_1g_1