Protocols: Prior to lysis, cells were stained with 1 ug/ml Trypan Blue per sample for 3 minutes, and counted using a Neubauer counting chamber. Then, the desired number of cells (1,000 or 10,000) was pelleted for 5 min at 200 rcf, resuspended in 50 µL of lysis buffer RLT Plus with 1% ß-mercaptoethanol Human embryonic kidney 293T (HEK293T) cells were cultured in DMEM media supplemented with 10% FBS and 100 U/ml Penicillin and 100 μg/ml Streptomycin. Cells were grown to 80% confluency, and harvested by trypsinization RNA was isolated using the bead extraction described in the prime-seq protocol RNA extraction using the Zymo Direct-zol RNA MicroPrep Kit The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66
ENA first public
2021-03-31
ENA last update
2021-02-25
External Id
SAMEA8138884
INSDC center alias
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC center name
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC first public
2021-03-31T04:07:30Z
INSDC last update
2021-02-25T16:10:06Z
INSDC status
public
Submitter Id
E-MTAB-10142:HEK_51
broker name
ArrayExpress
cell line
HEK293T
cell type
epithelial cell
common name
human
condition
No Incubation
developmental stage
embryo stage
disease
normal
genotype
wild type genotype
input cells (x thousand per 50 microliter lysate)
10
organism part
kidney
replicate
5
sample barcode
ACTGAGCGGTTCGA
sample name
E-MTAB-10142:HEK_51