Protocols: The brain was transferred to a dish with ice-cold PBS and placed in a 1 mm slicing matrix. Using steel blades, 5 coronal incisions were made. Biopsy punches were then taken from the striatum and the tissue was transferred to a 1.5 mL tube with 50 µL of lysis buffer, RLT Plus and 1% ß-mercaptoethanol. The tubes were snap frozen and stored at -80 ºC until needed. RNA extraction using the Zymo Direct-zol RNA MicroPrep Kit The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66
ENA first public
2021-03-31
ENA last update
2021-02-25
External Id
SAMEA8137229
INSDC center alias
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC center name
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC first public
2021-03-31T04:07:30Z
INSDC last update
2021-02-25T12:10:59Z
INSDC status
public
Submitter Id
E-MTAB-10140:Tissue_85
age
4
broker name
ArrayExpress
common name
house mouse
condition
Column
developmental stage
adult
genotype
wild type genotype
individual
1136
organism part
dorsomedial striatum
replicate
5
sample barcode
TGCAGCTAGAGTAGCACAGG
sample name
E-MTAB-10140:Tissue_85
sex
female
strain
C57BL/6