Protocols: Frozen vials of mouse embryonic stem cells (mESC) in Bambanker, and HEK293T cells were thawed and put into the corresponding lysis buffer DNA was extracted from 1 million mESCs using DNeasy Blood & Tissue Kit and RNA was extracted from 450,000 HEK293T cells using the Direct-zol RNA MicroPrep Kit The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66
ENA first public
2021-03-31
ENA last update
2021-02-25
External Id
SAMEA8138832
INSDC center alias
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC center name
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC first public
2021-03-31T04:07:30Z
INSDC last update
2021-02-25T15:54:35Z
INSDC status
public
Submitter Id
E-MTAB-10141:gDNA_3
broker name
ArrayExpress
cell line
HEK293T
cell type
epithelial cell
common name
human
condition
2RNA_2DNA
developmental stage
embryo stage
disease
normal
genotype
wild type genotype
organism part
kidney
replicate
1
sample barcode
ACTCGCTACCTGGACTTCTA
sample name
E-MTAB-10141:gDNA_3