Protocols: In accordance with the study protocol approved by institutional review boards of Universitair Ziekenhuis Brussel and Antwerp University Hospital, NSCLC patients with history of non-smoking (strictly never smoked or smoked less than 100 cigarettes in their life time) or former limited smoking (smoked less than five packed years with a smoking free interval of more than 20 years) were enrolled from 2013 to 2015 for the present study, which is called the Belgian FIELT-2 study. The majority of these patients had stage IV disease. Genomic DNA extraction from tumour and matched normal tissue were performed with the QIAamp DNA FFPE Tissue Kit and the QIAamp DNA Blood Midi/Maxi Kit (Qiagen Inc., Valencia, CA) respectively, according to the manufacturer's instructions. Quality and quantity of purified DNA was accessed by the NanoDrop (Fisher Thermo, Wilmington, DE) Whole-genome DNA libraries were created with the Illumina TruSeq sample preparation kit V2 according to manufacturer's instructions. Exonic fragments were captured with the Nimblegen SeqCap EZ Exome v3 or Agilent Sureselect Human All Exon V6 kit.
ENA-FIRST-PUBLIC
2021-03-30T17:18:20Z
ENA-LAST-UPDATE
2019-03-29T18:13:38Z
External Id
SAMEA5542392
INSDC center name
VIB Center for Cancer Biology (CCB) Laboratory for Translational Genetics Department of Human Genetics KULeuven
INSDC first public
2021-03-30T17:18:20Z
INSDC last update
2019-03-29T18:13:38Z
INSDC status
public
Submitter Id
E-MTAB-7833:FLT018_B
age
#N/A
broker name
ArrayExpress
common name
human
developmental stage
adult
environmental history
#N/A
individual
FLT018
organism part
germ-line (blood)
sample name
E-MTAB-7833:FLT018_B
scientific_name
Homo sapiens
sex
#N/A
tumor grading
#N/A