Protocols: Cells were cultured and differentiated into NPCs as described previously (Geuder et. al. 2021). One colony per sample was harvested and lysed in 100 µl RLT plus lysis buffer containing 1% beta-mercapto Ethanol and frozen at -80 °C. RNA extraction using carboxyl coated beads as described in the prime-seq protocol The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66
ENA first public
2021-03-31
ENA last update
2021-02-25
External Id
SAMEA8137188
INSDC center alias
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC center name
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC first public
2021-03-31T04:07:30Z
INSDC last update
2021-02-25T11:24:22Z
INSDC status
public
Submitter Id
E-MTAB-10133:Human3_NPC
broker name
ArrayExpress
cell line
N16_63AB2.2
cell type
neural progenitor cell
common name
human
disease
normal
genotype
wild type genotype
individual
Human3
progenitor cell type
induced pluripotent stem cell
sample barcode
GTAGGCGCATGA
sample name
E-MTAB-10133:Human3_NPC