Protocols: After 3 hours post-stimulation, all samples were collected with Trizol reagent (Ambion) and stored at -80ºC. NcgRNA and PAF1 KO cells were plated at a density of 6.0 x 105 cells per well in 6-well plates and incubated at 37ºC for 24 hours. Cells were then stimulated with poly I:C (2.0 μg/mL, Tocris), poly dA:dT (1.0 μg/mL, InvivoGen), LPS (1.0 μg/mL, eBioscience), IFN-β (50 U/mL, R&D Systems), and TNF-α (100 ng/mL, Gibco Thermofisher) at the given concentrations. For poly I:C and poly dA:dT, transfection was performed with Lipofectamine 2000 (ThermoFisher) per manufacturer instruction. Total cellular RNA was extracted and purified with the RNeasy mini kit (Qiagen) At the sequencing facility, RNA samples were prepared with the QuantSeq3' mRNA-Seq Library kit for Illumina (Lexogen GmbH), following a standard protocol (Moll et al., 2014)
ENA first public
2022-04-14
ENA last update
2022-04-14
External Id
SAMEA14025468
INSDC center alias
UNIVERSITY OF CALIFORNIA, DAVIS
INSDC center name
UNIVERSITY OF CALIFORNIA, DAVIS
INSDC first public
2022-04-14T16:31:47Z
INSDC last update
2022-04-14T16:31:47Z
INSDC status
public
Submitter Id
E-MTAB-11620:MWK016
broker name
ArrayExpress
cell line
A549
common name
human
developmental stage
adult
disease
lung adenocarcinoma
genotype
ncgRNA
organism part
lung
sample name
E-MTAB-11620:MWK016
stimulus
tumor necrosis factor-alpha