Protocols: Prior to lysis, cells were stained with 1 ug/ml Trypan Blue per sample for 3 minutes, and counted using a Neubauer counting chamber. Then, the desired number of cells (1,000 or 10,000) was pelleted for 5 min at 200 rcf, resuspended in 50 µL of lysis buffer RLT Plus with 1% ß-mercaptoethanol Human embryonic kidney 293T (HEK293T) cells were cultured in DMEM media supplemented with 10% FBS and 100 U/ml Penicillin and 100 μg/ml Streptomycin. Cells were grown to 80% confluency, and harvested by trypsinization RNA was isolated using the bead extraction described in the prime-seq protocol RNA extraction using the Zymo Direct-zol RNA MicroPrep Kit The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66
ENA first public
2021-03-31
ENA last update
2021-02-25
External Id
SAMEA8138838
INSDC center alias
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC center name
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC first public
2021-03-31T04:07:30Z
INSDC last update
2021-02-25T16:10:05Z
INSDC status
public
Submitter Id
E-MTAB-10142:HEK_1
broker name
ArrayExpress
cell line
HEK293T
cell type
epithelial cell
common name
human
condition
Incubation + ProtK
developmental stage
embryo stage
disease
normal
genotype
wild type genotype
input cells (x thousand per 50 microliter lysate)
1
organism part
kidney
replicate
1
sample barcode
CCTAAGACAAAGTT
sample name
E-MTAB-10142:HEK_1