Protocols: frozen PBMCs were gently thawed according to standard protocols, cells were counted using a hemicytometer and lysed in RLTplus lysis buffer with 1% beta mercapto ethanol at 4000 cells per 50µl RNA was isolated using the bead extraction described in the prime-seq protocol The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66
ENA first public
2021-03-31
ENA last update
2021-02-25
External Id
SAMEA8137195
INSDC center alias
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC center name
Ludwig-Maxilimians University, Munich Anthropology and Human Genomics Department Biology II
INSDC first public
2021-03-31T04:07:30Z
INSDC last update
2021-02-25T11:31:24Z
INSDC status
public
Submitter Id
E-MTAB-10138:PBMCs_69
broker name
ArrayExpress
cell line
PBMC LGC Standards (PCS-800-011)
cell type
mononuclear cell
common name
human
condition
Incubation + ProtK
developmental stage
adult
disease
normal
genotype
wild type genotype
input cell number per 50 microliter lysate
4000
organism part
blood
replicate
5
sample barcode
TCGACGTCGAGTAGCACAGG
sample name
E-MTAB-10138:PBMCs_69