Col-0 plants were treated with Tm
ecotype
Col-0
age
6-d-old
geo_loc_name
USA: East Lansing
tissue
Whole seedlings
genotype
Col-0
growth_protocol
Surface-sterilized seeds were plated on half-strength Linsmaier Skoog (LS) medium (Caisson Labs, Ontario, Canada) supplemented with 1% sucrose (Sigma-Aldrich, St. Louis, MO, USA), and 1.2% Agar (Acumedia, Lansing, MI, USA). Appropriate antibiotics was also added for screening of transgenic lines. After stratification in the dark at 4 °C for 2 days, plates were transferred to a controlled growth chamber with 80 μmol m−2 s−1 under 16 h light:8 h dark with temperature 22 °C.
phenotype
Wild-type withgrowth defect
sample_type
whole seedlings
treatment
Treated with Tunicamycin (500 ng/mL) for 24 h
BioSampleModel
Plant