Protocols: The leukemic samples were collected from the bone barrow aspirates of patients referred to the Hospital with suspect of leukemia. Bone marrow aspirates were used to separation of the mononuclear cells for morphological classication of blasts (FAB criteria) and immunophenotyping analysis (flow citometry) for precise leukemia subtype definition. The same mononuclear fraction (an aliquot) was used to the microRNA sequencing experiments and analysis. We performed total RNA extraction using the TRIzol reagent (Invitrogen, Carlsbad, California), following the manufacturer's instructions. miRNA library construction was performed starting from 1 μg of each RNA sample using the TruSeq Small RNA Library Prep kit (Illumina, San Diego, California), following manufacturer's instructions.
ENA first public
2022-04-14
ENA last update
2022-04-14
External Id
SAMEA14025665
INSDC center alias
Aggeu Magalhaes Institute/Oswaldo Cruz Foundation
INSDC center name
Aggeu Magalhaes Institute/Oswaldo Cruz Foundation
INSDC first public
2022-04-14T16:31:47Z
INSDC last update
2022-04-14T16:31:47Z
INSDC status
public
Submitter Id
E-MTAB-11621:Acute leukemia 11 (LA11), lanes 1 and 2
age
5
broker name
ArrayExpress
common name
human
developmental stage
juvenile stage
disease
B-cell acute lymphoblastic leukemia
organism part
bone marrow
sample name
E-MTAB-11621:Acute leukemia 11 (LA11), lanes 1 and 2
sex
male