Protocols: PBMCs separation was performed by using the Ficoll-Paque (Ficoll Plaque PLUS - GE Healthcare Life Sciences, Piscataway, USA) and the RNA was extracted using the TRIzol reagent (TRIzol Reagent, Invitrogen Life Tecnologies, Carlsband, CA, USA), according to the instructions provided by the manufacturer. For RNA extraction, each whole blood sample (approx. 10 ml) was processed within 2 hours of collection and the RNA was stored at -80 °C. Indexed libraries were prepared from 1 µg/ea purified RNA with TruSeq Stranded mRNA (Illumina) Library Prep Kit, according to the manufacturer's instructions.
ENA first public
2021-06-28
ENA last update
2021-06-28
External Id
SAMEA9233009
INSDC center alias
Genomix4Life SRL
INSDC center name
Genomix4Life SRL
INSDC first public
2021-06-28T00:15:10Z
INSDC last update
2021-06-28T00:15:10Z
INSDC status
public
Submitter Id
E-MTAB-10604:DG_5480_DOWN
broker name
ArrayExpress
cell type
peripheral blood mononuclear cell
common name
human
disease
Down syndrome
genotype
trisomy 21
organism part
blood
sample name
E-MTAB-10604:DG_5480_DOWN