Protocols: Samples were collected from upper systemic leaves of TRVs infected plants at one week post infection and three weeks post infection. The collected samples were flash frozen in liquid nitrogen. Plants were grown in Levington F2+S professional growth compost in a controlled growth chamber (SANYO/Panasonic) at 22°C with 16-h light and 8-h dark periods for 4 weeks. Plants were infected at 4 weeks of age. After viral inoculations, the infected plants were maintained at 22°C for 2 days and then shifted to 29°C for long term. Total nucleic acid (TNA) extracts were prepared from leaf tissue by phenol-chloroform extraction as described in White and Kaper, 1989. Small RNA libraries were prepared from 1 μg of TNA using the Illumina TruSeq Small RNA Library Prep Kit and sequenced on the Illumina HiSeq 2500 platform with 50-base single end reads at Edinburgh Genomics (Edinburgh, UK).
ENA first public
2021-06-28
ENA last update
2021-06-28
External Id
SAMEA7087012
INSDC center alias
Institute of Molecular Plant Sciences The University of Edinburgh
INSDC center name
Institute of Molecular Plant Sciences The University of Edinburgh
INSDC first public
2021-06-28T00:21:42Z
INSDC last update
2021-06-28T00:21:42Z
INSDC status
public
Submitter Id
E-MTAB-9394:TRV-GFP-29C-21
age
7
broker name
ArrayExpress
ecotype
16c
environmental history
long day length regimen
environmental stress
infected with TRV-GFP (modified TRV)
genotype
wild type genotype
growth condition
Before infection: germinated and maintained at 22 deg for 4 weeks; post infection: maintained at 22 deg for 2 days and then shifted to 29 deg for long term.
infect
Tobacco rattle virus
organism part
leaf
sample name
E-MTAB-9394:TRV-GFP-29C-21