RNA was evaluated using the 2100 Bioanalyzer RNA 6000 Pico assay (Agilent). Amplified cDNA was prepared using the Ovation RNA-Seq System V2 (Nugen).  Amplified cDNA was sheared on an LE220 ultrasonicator (Covaris). Libraries were prepared from sheared DNA with the HyperPrep Library Preparation Kit (Roche PN 07962363001). Libraries were analyzed for insert size distribution using the 2100 BioAnalyzer High Sensitivity kit (Agilent), 4200 TapeStation D1000 ScreenTape assay (Agilent), or 5300 Fragment Analyzer NGS fragment kit (Agilent). Libraries were quantified using the Quant-iT PicoGreen ds DNA assay (ThermoFisher) or by low pass sequencing with a MiSeq nano kit (Illumina). Paired end 100 cycle sequencing was performed on a NovaSeq 6000 (Illumina).
strain
C57BL/6J
isolate
24
breed
not collected
cultivar
not collected
ecotype
not collected
age
12 Months
replicate
biological replicate 3
sex
not provided
tissue
Bone Marrow
biomaterial_provider
Kim E. Nichols
cell_type
LSK
genotype
Etv6+/-
geo_loc_name
NA
collection_date
NA
BioSampleModel
Model organism or animal