Protocols: HCT116 cells were plated at 6cm cells and 24 hours later transfected with control or RBM42 siRNA. 48 hours after siRNA transfection, cells were treated with 20 micromolar VP16 (etoposide) for 12 hours. Cells were directly lysed using TRIzol reagent (Ambion) for RNA extraction. HCT116 cells were grown in a humidified 37C incubator (5% CO2) in RPMI-1640 medium (Gibco) supplemented with 10% heat-inactivated FBS, 2mM L-glutamine (Gibco), 100unit/mL penicillin and 100μg/mL streptomycin (Gibco). siRNA transfections were performed using Lipofectamine™ RNAiMAX (Invitrogen) according to the manufacturer's instructions. Total RNA was extracted from cells using TRIzol reagent according to the manufacturer's instructions (Ambion). RNA-seq libraries were prepared at the Crown Genomics institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science using TruSeq mRNA library preparation kit (Illumina). Libraries were quantified by Qubit (Thermo fisher scientific) and TapeStation (Agilent).
ENA-FIRST-PUBLIC
2022-09-15
ENA-LAST-UPDATE
2022-09-15
External Id
SAMEA110188217
INSDC center alias
Faculty of Biology - Technion Israel Institute of Technology
INSDC center name
Faculty of Biology - Technion Israel Institute of Technology
INSDC first public
2022-09-15T00:14:43Z
INSDC last update
2022-09-15T00:14:43Z
INSDC status
public
Submitter Id
E-MTAB-11877:ctrl_VP16_B
broker name
ArrayExpress
cell_line
HCT116
common name
human
developmental stage
adult
disease
colorectal carcinoma
genotype
wild type genotype
organism part
colon
response to treatment
20 micromolar VP16 (etoposide) - 12 hours
sample name
E-MTAB-11877:ctrl_VP16_B