Protocols: 0.5g leaf tissue was fixed in 1% formaldehyde (v/v) and 0.01% Triton-X (v/v) for 20 min using vacuum infiltration. The reaction was quenched by adding glycine to a final concentration of 125 mM for 10 min using vacuum infiltration. The leaf tissue was rinsed 3 times with MilliQ water, patted dry and flash frozen in liquid Nitrogen. Plants were germinated and grown in F2 compost and transferred to 16h light and 8h dark at 22C day temperature and 18C night, 70% humidity Chromatin extraction, digestion, reverse cross-linking and DNA purification were carried out as described in Liu et al, 2017. PMID: 28848243 As described in Liu et al, 2017. PMID: 28848243
ENA first public
2021-06-27
ENA last update
2021-06-27
External Id
SAMEA9232312
INSDC center alias
Department of Plant Sciences University of Cambridge
INSDC center name
Department of Plant Sciences University of Cambridge
INSDC first public
2021-06-27T00:12:56Z
INSDC last update
2021-06-27T00:12:56Z
INSDC status
public
Submitter Id
E-MTAB-10574:Sample 6
age
4
broker name
ArrayExpress
common name
tomato
cultivar
Lukullus
developmental stage
sporophyte vegetative stage
epigenotype
TAB2sulf
genotype
sulfurea
organism part
leaf
sample name
E-MTAB-10574:Sample 6