strain
Caulobacter crescentus CB15N
host
not applicable
collection_date
2020-10-21
geo_loc_name
not applicable
sample_type
bacterial cell culture
genotype
PlacI-lacI(hfa locus); Plac-dnaA(dnaA locus);I-SceI site(after CCNA_00727)::tetR; Pvan-I-SceI::chlorR; parSpMT1site (after CCNA_00747)::specR; deletion of recN::gentR
treatment_protocol
Caulobacter cells harboring a deletion of recN were cultured at 30oC in PYE media. To allow replication culture was supplemented with 0.5mM IPTG to induce expression of DnaA. For isolation of non-repliacting swarmer cells, culture was incubated without IPTG for 1.5h to deplete DnaA. Isolated swarmers were released in media without IPTG, DSB was induced by supplementing media with 500µM vanillate. Cells were pelleted 1h after DSB induction and genomic DNA was isolated using DNeasy Blood and Tissue kit from Qiagen. Whole genome sequencing was carried out for this sample using Illumina sequencing (NGS facility, NCBS, Bangalore).
BioSampleModel
Microbe, viral or environmental