Protocols: Tumors were removed from the tongue of C57BL6 and were cut into small pieces and inflated with digestion solution containing 1 mg/mL Collagenase D (Roche) and 0.2 mg/mL DNase I (Roche) 2% inactivated fetal bovine serum in RPMI, at 37°C for 2 hours. Upon completion of digestion, 92 µL of EDTA 54 mM was added and the samples were vortexed at maximal speed for 30 seconds. The resulting cell suspensions were passed through a 70 µm and 40 µm cell strainer, washed with PBS and placed in culture. cultured cells were dissolved in the TRizol reagent (Life Technologies) for total RNA extraction with Qiagen kit. Total RNAseqV2 library kit (Thermofisher Scientific)
ENA first public
2021-06-28
ENA last update
2021-06-28
External Id
SAMEA9233596
INSDC center alias
University of Strasbourg
INSDC center name
university of strasbourg
INSDC first public
2021-06-28T00:15:10Z
INSDC last update
2021-06-28T00:15:10Z
INSDC status
public
Submitter Id
E-MTAB-10614:OSCC2G_4
broker name
ArrayExpress
common name
house mouse
developmental stage
adult
genotype
wild type genotype
growth condition
isolated tumour cells cultured until passage 9
individual
2
organism part
tongue
replicate
2
sample name
E-MTAB-10614:OSCC2G_4
sampling site
neoplasm
strain
C57BL6