A recombinant virus was successfully generated by homologous recombination through co-transfection with the transfer vector and then infected with ASFV GZ201801 wild type virus. The deletion mutant was purified by successive rounds of plaque purification and limiting dilution. The purified recombinant virus was named ASFV-GZdeltaI73R, and its genome sequence was analysed by nanopore sequencing, confirming the successful deletion of I73R in the recombinant virus.
isolate
virus suspension
host
Sus scrofa
collection_date
2021-01-01
geo_loc_name
China
isolation_source
cells
strain
ASFV GZ201801
breed
not collected
cultivar
not collected
BioSampleModel
Viral