Protocols: Glioblastoma Multiforme (GBM) tissues were collected from patients who underwent surgery. From these Glioblastoma Multiforme stem-like cells (GSCs) were isolated. For Dependent growth factors Glioblastoma Multiforme stem-like cells (D-GSCs), single-cell suspension was plated in NeuroCult NS-A medium containing 20 ng/ml of epidermal growth factor (EGF) and 10 ng/ml of fibroblast growth factor (FGF2) and cultured in humidified atmosphere at 37°C, 5% O2 and 5% CO2. For Independent growth factors Glioblastoma Multiforme stem-like cells (I-GSCs), single cell suspension was plated in NeuroCult NS-A medium without growth factors and cultured in humidified atmosphere at 37°C, 5% O2 and 5% CO2. Genomic DNA was extracted using the Blood & Cell Culture DNA Midi Kit (Qiagen). Truseq custom amplicon sample kit (Illumina Inc, CA, USA) and Ampliseq kit (Illumina Inc, CA, USA) for targeted capture and library preparation was prepared from 250ng or 20ng of double stranded DNA accordingly to manufacturers instructions.
ENA-FIRST-PUBLIC
2022-04-15
ENA-LAST-UPDATE
2022-04-15
External Id
SAMEA8691654
INSDC center alias
"Institute for Stem Cell Biology, Regenerative Medicine and Innovative Therapies (ISBReMIT) - IRCCS ""Casa Sollievo della Sofferenza"""
INSDC center name
"Institute for Stem Cell Biology, Regenerative Medicine and Innovative Therapies (ISBReMIT) - IRCCS ""Casa Sollievo della Sofferenza"""
INSDC first public
2022-04-15T00:14:47Z
INSDC last update
2022-04-15T00:14:47Z
INSDC status
public
Submitter Id
E-MTAB-10418:D-GSCs-CL#26
broker name
ArrayExpress
cell_line
glioblastoma multiforme cell line
cell_type
stem-like cells
common name
human
developmental stage
adult
disease
glioblastoma multiforme
genotype
wild type genotype
growth condition
20 ng/ml of epidermal growth factor (EGF) and 10 ng/ml of fibroblast growth factor (FGF2)
individual
Patient 1
isolate
not applicable
organism part
brain
sample name
E-MTAB-10418:D-GSCs-CL#26