ENA first public
2021-05-03
ENA last update
2020-04-14
External Id
SAMEA6675623
INSDC center alias
WAGENINGEN UNIVERSITY (Bioinformatics)
INSDC center name
WAGENINGEN UNIVERSITY (Bioinformatics)
INSDC first public
2021-05-03T00:24:49Z
INSDC last update
2020-04-14T15:32:08Z
INSDC status
public
SRA accession
ERS4425159
Sample Name
parasponia_andersonii16
Submitter Id
parasponia_andersonii16
air temperature regimen
28degrees
collection date
2019
culture rooting medium
SH salts 3.2 g.L-1, and SH vitamins 1 g.L-1
estimated size
550mb
genetic modification
CRISPR-Cas9 knockout of PanNOOT1
genotype
PanNOOT1_A10
geographic location (country and/or sea)
Netherlands
geographic location (latitude)
51.9669161323
geographic location (longitude)
5.65515904602
growth facility
growth chamber
light regimen
16/8h day/night
mineral nutrient regimen
nutritive solution (NH4, 1 mmol.L-1; K, 5.9 mmol.L-1; Ca; 2.7 mmol.L-1; Mg, 0.8 mmol.L-1; NO3, 9 mmol.L-1; SO4, 1.9 mmol.L-1; P, 1.1 mmol.L-1; Fe, 15 µmol.L-1; Mn, 5 µmol.L-1; Zn, 5 µmol.L-1; B, 3 µmol.L-1; Cu, 0.5 µmol.L-1; Mo, 0.5 µmol.L-1; pH: 5.8)
plant developmental stage
stem internode 3
plant growth medium
Schenk & Hildebrandt (SH)
plant structure
stem
ploidy
2n
rooting medium carbon
sucrose 10 g.L-1
rooting medium pH
5.8
rooting medium regulators
IBA 1 µg.mL-1; NAA 0.1 µg.mL-1; MES 3 mM
rooting medium solidifier
Daishin agar
sample health state
healthy
title
Reverse genetics using CRISPR-Cas9 in the tropical tree Parasponia andersonii revealed a promotive role for PanNODULE ROOT1 in stem secondary growth
watering regimen
2times/week water or nutritive solution