Broilers in the three groups were fed basal diet, basal diet + lipopeptide biosurfactant (300 mg/kg) and basal diet +APG surfactant (300 mg/kg), respectively. basal diet is control. On day 14 and day 28, the samples were collected. In each group, three biological samples were collected. The sequencing of each biological sample contains three technical repetitions. A total of 54 subsamples were included.