The ML5 transposon library aliquot grown in 25 mL M9 + 20 mM D-glucose  and grown to an OD600 of ~1.0 (shaking 225 RPM)  (Sample 1C) was used to inoculate 25 mL M9 + 20 mM D-glucose (125 baffled flask)  and grown to an OD600 of ~1.0 (shaking 225 RPM) prior to pelleting and storing at -80 ˚C