IRF8PRO_CN_MON_ASO
Protocols: human PBMCs are collected and sorted CD14+ monocyte,after neon mediated electro-transfection,total 1x10^7 monocyte cells are collected and fixed by 37% formaldehyde1 DNA was purified using phenol-chloroform and further purified with QIAquick PCR purification kit (28106, Qiagen). The DNA concentration was detected by Qubit (ThermoFisher)