yjhX167-3
Protocols: Colonies selected from mutant screen for overnight cultures grown in LB at 37C. Bacterial cultures were grown at 37 °C in LB medium. 100 μl of an overnight culture was washed and plated on M9+0.2% lactose agar. Spontaneous survivors identified by whole genome sequencing Genomic DNA was prepared from overnight culture using a DNeasy Blood and Tissue Kit (Qiagen). A DNA library was prepared using a Nextera kit (Illumina).