LPS 2
Protocols: PBS- and LPS-treated microglia were transfected with either miR-106b or inhibitor controls for 2 days. RNAs were isolated from cells using Qiagen RNeasy mini kit according to the manufacturer's instructions. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® following manufacturer's instructions and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS following manufacturer's instructions.